TWI326600B - Use of genipin as skin marker pigment - Google Patents

Description

1326600 •··············································································· The invention still further provides a human or animal skin marking composition comprising scorpionin as a labeling dye, and a pharmaceutically acceptable carrier or diluent. [Prior Art] Genipin is a hydrolyzate of genip〇side, which is present in the fruit of Gardenia jasmin〇ides ELUS. In biomedicine, it is currently known that scorpion has: (1)

The research report pointed out that the amino acid will form a blue pigment after the reaction. Therefore, due to its color durability and sensitivity, the scorpion has 1326600 • I * t, which can be used as a fingerprint instead of ninhydrin. Analysis (fingerprint)

Acta 2003, 480, 267-274.)

South Asia has been widely used for food dyeing. Patients (especially cancer patients) must use paint to mark the area or site to be tested and/or radiotherapy during regular testing and/or radiation therapy. Currently, commercially available oil-based markers are commonly used in hospitals. 'For example, a conventional marker or a singular pen is used for calibration. However, the mark made by the conventional oil-based marker pen on the body is easy to be removed or faded when the patient takes a bath or wipe, which causes difficulty in identification, and therefore often makes the mark remain on the body for a long time, often It is necessary to endure the inconvenience of being unable to clean the body, especially in the hot summer temperatures. Therefore, the inventor of the present invention proposed to apply scorpion scorpion to human or animal skin markers. The experiment of the invention proves that the standard composition prepared by using vibrin as a dye can be kept on the skin for a long period of time, and is not removed by washing or wiping, and has industrial utilization 132, 6600. The present invention will be described in part below, or may be apparent from the description. The object of the present invention is to provide a new use of genidin as a human skin f animal skin marking dye. The "skin mark" described in this document is not limited to the doctor's advice, and f ~r w 亦可 may also be used for the purpose of allowing the dye to remain on the skin for a longer period of time. For example, it is applied to makeup, animal marking and other human or animal identification. Another object of the present invention is to provide a marker composition for use in human or animal skin which comprises 栀qG, and a pharmaceutically acceptable carrier or diluent. Other than the specific energy H ^ . , the heart-shaped, the labeling composition of the present invention may further comprise an additional dye, in the form of a body, for use in the present invention. The other item has a body 1 ' and the diluent is an oily solvent. In another specific aspect, it is used in the present solvent. The diluent of the composition is alcohol. Other characteristics of the present invention The disadvantages will become apparent after the detailed disclosure below. The present invention will be described in detail with reference to the particular embodiments of the present invention, which are not intended to limit the scope of the invention. There are aptitudes to include these and other changes and variations. %Example 1 · The color and color fading of the vibrin solution on the skin is dissolved in lOinl alcohol to form a solution of 1% scorpionin. The hazelnut solution was diluted to a final concentration of 7%, 5%, 2%, and 200 ul each; and the commercially available lion ink 200U1 was also used as the control group. The concentrations were taken at the tip and applied to the back of the hand. The scorpion is photographed on the skin, and the presence of the scorpion solution and the commercially available ink on the skin. The results are shown in Figure 1. • The photo record of the first figure shows the scorpion 12 hours after the solution was applied to the skin, it gradually appeared blue, and after the eleventh day of the experimental period, it still retained its recognizable chromaticity on the skin. Otherwise, the commercially available lion ink itself With color However, on the third day after application to the skin, its original color has faded to almost no recognition of its marked position and has completely disappeared on the fourth day. 132660.0 * · < » 1 $ Example 2. Osmanthus preparation Oil-forming and alcohol-based marker composition! The body surface coloration test is firstly configured with 10% scorpion solution using 60% alcohol. 1 gram of scorpion (+Vako' product number: 078 — 03021) is added to 9ml. 60% alcohol, as for the reaction in a 37 °C warm water tank for about 1 , minutes, when the scorpion powder is dissolved, the solution should be kept in the dark. The resulting 〇% scorpion solution is alcoholic. Ink (Lion, "Dividing a dye, resin, melting", or oily ink • water (lion, ingredient - dye, resin, solvent) is diluted as shown in the table below, and is formulated into different concentrations of the mark The final degree of the resulting marker composition is 〇%, 2%, 5% and 栀~~~

Degree 60% alcohol Ink (ml) total volume (ml)

Point on the skin, then 'paste the collected photo into a π-forming marker composition to take a normal bath in a capillary mother' and photograph 132660.0 I * · β at a fixed time to analyze 'to assess alcoholicity or degree and color loss . Results: The oily marker composition was attached to the skin. As shown in Fig. 2, it was found that the oily marker composition exhibited a diffusion phenomenon by using hair and spots on the skin, and the alcoholic marker composition did not. As a result, oily; I® W " does not spread about 3 times the original radius. In terms of durability, it is better to use an oil-based marker composition, even if the concentration of the marker composition as low as 2% is clearly visible after 14 days, and the image will be collected every day using the image software. For the analysis, a commercially available marker pen (singular pen) dye was also used as a comparison group. From the numerical graph shown in ^2, it is found that whether the marking composition of the present invention or the commercially available singular pen ink's color gradually fades with time, the coloring of the marking composition of the present invention It is mild, and on the 14th day, about 40% of the original color is attached to the body surface. Example 3. Color drop test of the marking composition (oily) of the present invention after rinsing at different times As described in Example 1, the composition was prepared to have a final concentration of 0% (oily) marking composition. 4, 6, 8 and 1 hour before the bath of the volunteers were tested, the labeled composition of Yuancheng was painted on the skin and fixed at a time of 10 4 »

A point photo. The photos collected at each time point are divided into image software to evaluate the degree of adhesion and fading of the dye on the skin, and the stimuli of the marking composition of the present invention can be evaluated. And whether it will cause allergies. Result: As shown in the fourth figure, the mark composition of the present invention is applied to the skin 10 hours after the start of the bath, the color drop is the slightest, and the color rendering effect is also the best. The labeling composition, as the reagent stays in the skin for a shorter period of time, also indirectly affects the subsequent coloring time. Unintentionally limited by any theory, scorpion is known as a natural cross-linking: it is presumed to be coated. After covering the skin, it will have a parental effect with the protein shell in the epidermis or dermis, which is beneficial to its stable presence on the skin. Therefore, after the composition of the hair on the skin is applied to the skin, it takes time and skin to reverse ^ w When the reaction effect is not completed, it is washed by the detergent, which will disperse the dye of the dye, and reduce the pigment content on the surface of the skin, and the color effect is poor. Therefore, the mark of the present invention After the composition is applied, it should be taken at least half a day before bathing in order to maintain its labeling effect. X, scorpionin is originally colorless, and t is blue when combined with the amino acid of the skin protein composition (see, Example 1) The result shown), therefore, in order to enable immediate display of the '5-position' in the skin-before, an additional conventional dye can be added to the skin-marking composition of the present invention, and the label can be made by the action of the vibrin. Can be longer

The I3260UU period is stable on the skin. The experimental gentleman of the above and above. GeniPin is used as a dye for human or animal skin markings. It is superior to the skin durability of conventional or commercially available marking dyes, and the original marking position can still be seen 10 days after the punching. . Furthermore, since the blue scorpion is a safe food coloring, and the scorpion is known as a non-toxic natural crosslinking agent, the standard of the present invention is also used on the skin. Does not produce allergies and, or irritating advantages.

12 132660.0 • < * [Simple description of the diagram] The first picture shows the boswell solution (10%, 7%, 5% ' 2% and 1%), and the commercially available lion ink (as the control group), After being applied to the skin (point on the skin with a tip), at the specified time point (〇, 1, 2, 2 5, 12 hours, 1, 2, 3, 4, 5, 6, 7, 8) , 9, 10, U, and 12 days) Photographs of the degree of coloration and discoloration. The picture is from left to right for 5 cards (lion ink), 10%, 7%, 5%, 2% and 1% scorpion solution. The second figure shows the adhesion of the alcoholic and oily marking compositions of the present invention to the skin (with capillary spots on the skin) I at the specified time points (days 2, 3, 10 and 14). Photograph of degree and discoloration. The third picture is a daily analysis of the photos collected by the image software using the image software. A is the result of testing with an oily target. B is the result of testing with an alcohol-labeled composition. ° Adhesion degree and fading phenomenon [Description of main component symbols]: The chart is not applied to the skin after 1 〇 hours of application, and after the designated time, the temple begins. Correction is as follows: ^ 占 (Dijon, 6 and Μ天) observation 13

Claims (1)

April 9, 1999 revised version, patent application garden: embryo £9 - genipin (genipin) used as a new use of human or animal skin marker dyes. A marker composition for use on human or animal skin comprising scorpionin as a marker dye and a pharmaceutically acceptable carrier or diluent. The marking composition according to item 2 of the patent application further comprises an additional dye suitable for use on human or animal skin. According to the marking composition of claim 2, wherein the diluent is an oily solvent suitable for human or animal skin. β. According to the application of the full-time (4) 2 item marking silk, wherein the diluent is suitable for human or animal skin. Alcoholic solvent.