1280278 九、發明說明： 【發明所屬之技術領域】 本發明杏鮑撕品種係-種育成之食用微生物及其栽培生產 方法，杏誠在台灣別稱為干題（學名為伙⑽加 eryngii (DC· :Fr· ) Qu6l·)，係發現於歐洲南部、非洲北部以及中亞地區 高山平原、沙漠地區的-種品質優良的大翻f傘菌；由於杏鮑 兹菌肉肥厚、親爽π、耐於運輸、保鮮期長，故市場上的杏鮑 釦十刀文到歡迎，本發明運用雜交優勢育成杏鮑菇新品種及其獨 斗寸的栽培方式，能夠育成具獨特食用口感之杏鮑菇微生物，配合 栽培生產方法能有效提高杏鮑菇生產產量及提高杏鮑菇品質。 【先前技術】 按，由於現代科技搭配農業生產的技術水準不斷進步提昇， 而精緻化農業的生產力、消費力更是驚人地成長，所以，各式菇 類菌種培養技術已是目前精緻化農業、飲食生產業十分重要的技 術之一，目前一般所見的菇類產品、食用菌類產品便十分的需要 優良的囷株或品種’以及培養技術的搭配’但是，由於菌種培養 技術的步驟、生產方式各有不同，故菌種本身的特性是否為較佳 的微生物菌株、是否具有較佳的培養技術及成效、與是否節省成 本便成為研發者最為重視的問題；因此，如何開發出一種具有前 述功效的全新菇類微生物及栽培生產技術，早已變成研發者與製 1280278 造商最想克服__題，社述内容中所呈現關題也是本宰 發明人研發本案的動機。 、 一般目前最常見的翻釀培方式是以採用太空包以人工植菌 的生產方式’由㈣往生產方式人卫及雛口耳相傳，一直 以來並未有祕化的杏誠纽模式，故齡讎杏麟的業者 仍有下觸題爾改進，在此——分析解說如下： 其一，尚未系統化的作業方式十分浪費人力，自動化機械的 使用並未十分普遍，且沒有讓生產流程連貫—致，而各項人力的 成本居南不下。 其二’由於傳統生產者絲營造最佳城環境，以致每一批 杏腿生產的產品會有獨的產量、剌彡，甚至會有苦味的口感， 無法達到-致的出品品質’是故，針對以上缺陷，在求理想、食 用與進步之今日，誠為一亟待努力追求改善之目標。 -般目前最常見的杏鮑益（學名·他㈣加町袖· (DC. .Fr·) Quel·)微生物例如·· ATCC 36G47及HOLLAND 150兩菌 株’ 4者（ATCC 36047 )之菌株原始生長地為東歐之捷克斯拉夫， 後者（HOLLAND150)縣生長地未詳(如有需要，可代查詢）。以 上兩菌株經以木屑與米糠混合物瓶栽所觀察之出菇特性舆園藝性 狀況明如下（睛-併茶閱附件），而下述内容也揭露出二種常見 1280278 習知杏飽姑微生物菌株存在的問題： 繼陳_娜趣15__，在—般栽培 官理條件下’出錄不整齊，容易產生過多子實體 ，以致紐較為細小等缺點；除非育_境—直處於非常潮澄之 狀況或不斷利用離心式噴霧機加渔，造成在菌傘表面有水滴存 在:否則概腿7g_傘表面対絲瘤出現，但仍偶而有 較深色之練紐缝成，概·47躲微生物_尤其菌柄 in刀特另'i堅只，負地非常細腻，口感特佳，耐低溫儲藏，貨架 儲存壽命長，惟產量與越效率較胍L_⑽為低，本菌株對出 Erwinia細菌引起之軟腐病及由伽祕咖^⑽彻.知菌引起 之蛛網病（Cobweb disease)均無抗病性。 HOLLAND 150菌株：出菇較ATCC 36〇47為快，在一般栽培管理 條件下，具有出御交為整齊之優點，比ATCC 36〇47菌株不易產生 k夕子實體，因此其4體一般為中型至大型，在與Atcc 36047菌 株相同環境條件下栽培時，H〇LLAND 15〇菌株的菌傘表面較容易 產生疣狀瘤，以致菇體外觀不佳，且H〇LLAND 15〇菌株之菌傘較 為深色且易碎裂，而且該HOLLAND 150的微生物組織較為鬆軟（包 括菌傘及菌柄），不耐低溫儲藏，貨架儲存壽命短，惟H〇LLAND 15〇 il株的菇體質地十分細腻，菇體較為大型，且其產量與產菇效率 句較别囷株（ATCC 36047)為高，本菌株對由細菌Erwinia引 病认反由Cladobotryum dendroides儀Μ起之蛛蜗病 1280278 (Cobweb disease)亦毫無抗病性。 有鑑於斯，本案發明人驗詳思細索，並積多年從事各種兹 類菌種培養與相關兹類菌種之研究與開發之經驗，終而得以開發 出一種生長速度快、品質佳、出菇容易、成品整齊、產量高及^ 物效率高綠_伽微生物，而本發明食驗生物_獨特的 栽培方法後可赠得g絲生長速度快、錢料、成品整齊、產 量高及生物效率高的杏鮑菇之食用微生物。 【發明内容】 本發明之主要目的，在於提供—触長速度快、品質較佳的 杏鮑兹育成之食賴生物，本發明顧系統化、娜育成環境及 精準的雜交雙㈣培養技術，生產出以優異雜交雙核體成長的杏 鮑兹之食職生物’本發明運__育種方法育成喊較佳之 艮用U生物’具有使杏鮑生長速度快、勘彡豐滿粗大、組織堅 實白細、食用口感具彈性的優點。 本發明之次要目的，在於提供一種出兹容易、成品整齊、產 兹效率高的杏鮑賊培生產方法，本發明利用⑴.母種培養步 驟，⑵.栽培用木麟選步驟；⑶栽培材料混合及瓶裝步驟； 1280278 ⑷.栽培材料之殺菌及接種步驟；⑸ 出兹步驟，·⑺.採收及包裝步術個步驟刺激 較佳品質之食财姓物，且可 i生產具有 成品整齊，更且w^^ 偏出#速度，不但使出菇 3〇%)。 遍赠率（編他量大幅增加達 法：所提供之娜食用微生物育成技術舆其栽培生產方 、杏飽兹食用微生物之育成·· ⑴·進行雜交親本原種之培養繁殖及保存：雜交 ==及胍_ 150嘛議__ 序進」：:雜Γ親本之原種製作及栽培菌種之製作與栽培:依 二原種之4作、b.栽培種之製作、c.栽培用菌種之栽 粉頭,：20 : 20之乾重比，太米糖： 肖了使用山頁麻或其他非硬木類 ，姻碳_整阳值介於6.5至7.〇之間，而水 伤3里I利3〜6 5% ;該栽培用菌種採用木顧種所使用 之栽培基_方為木屑：米糠：粉頭=50 ·· 15 : 35之乾重比，木 屑可使用山κ麻、楓木或其他麵木類之闊葉樹種木屑或其混合 9 1280278 物 ，其餘製作過程與原種補；域培用《難得後，即可開蓋去 皮’去皮後之栽培㈣種移人純室，城室之溫度調整於15〜 16°C ;最後再進行擔孢子之收集備用。 (3).進行擔孢子之單孢分離及單孢菌株之確認與培養 ••採用孢子稀釋培養分離法分離單孢_，經2 5t：定溫培養4 至5天後切下含單獨落之洋菜培養基，並置於試管培養基以 2 5 °C定溫培養。 ⑷·進行單孢@株間之雜交與雜交雙核體之確認、培養及保 存··獲得之單孢g株經繁殖於PDAS管後，_交親本之單抱 菌株以相隔0· 5至1公分距離同時接種於同一試管培養基上，置於 25 C定溫培養25至30天後，兩g落相互融合且均失去其個體性， 分別於兩g落融合處或鋪處箱微鏡鏡檢驗證自絲產生扣子 體’將雜X成功之g絲體移丨培養於新培養基，並以前述方法做 定期移植及保存。 (5)·進行雜交雙核體之首次栽培出菇篩選：經確認之雜交雙 核體，先製作成木屑原種，再製作成栽培用菌種；比較從不同雙 核體之產減減特性，篩選越效率高、碰外觀佳、兹體組 織結實度良好、錢早而整齊者，供做再進—步栽栽試驗比較。 ⑹·進行優異雜交雙核體之再栽培及出簡性触：從首次 栽培试驗4魏得紐異之雙核體，再製械栽培㈣種，選取 10 1280278 再擴大栽培規模，多 以獲得最優異杏鮑菇 具有特色與栽培經濟價值高之雜交雙核體， -入與雜交親本或目前商業栽培品種做比較， 育成之食用微生物。 二、杏鮑菇食用微生物栽培生產方法則利用下列七個主要步 驟^請參閱第-圖，依據本發明所提供之食用微生物 栽培生產方法，係包括下列之製造步驟為其特徵者： (1) ·母種培養步驟； (2) ·栽培用木屑篩選步驟； (3) ·栽培材料混合及瓶裝步驟； (4) ·栽培材料之殺菌及接種步驟； (5) ·菌種培養步驟； (6) ·刺激出菇步驟； (7) ·採收及包裝步驟； 運用上述7個步驟，可以增加產量，且配合(6)·刺激出菇步 驟’可以有效控制每一栽培瓶所產生的菇體數目，確保生產品質 及產量之穩定。 【實施方式】 有關本案發明為達成上述目的、技術，所採用之詳細手段及 其他功效，兹列舉一較佳可實施例並配合圖式詳細說明如後，相 111280278 IX. Description of the invention: [Technical field to which the invention belongs] The edible aphid of the apricot tortoise variety of the invention-cultivation and cultivation and production method thereof, Xingcheng is also called a dry problem in Taiwan (scientific name is (10) plus eryngii (DC) · :Fr· ) Qu6l·), is a kind of high-quality big-turned toadstool found in the mountains of northern Europe, northern Africa, and the alpine plains and desert areas of Central Asia. Because of the hypertrophy of the abalone, the fragrant π, It is resistant to transportation and has a long shelf life. Therefore, the apricot abalone in the market is welcome. The invention uses the hybrid advantage to cultivate a new variety of Pleurotus eryngii and its cultivation method, which can cultivate apricot abalone with unique taste. Mushroom microbes, combined with cultivation methods, can effectively increase the production yield of Pleurotus eryngii and improve the quality of Pleurotus eryngii. [Prior Art] According to the continuous advancement and improvement of the technological level of modern technology and agricultural production, the productivity and consumption power of refined agriculture are staggeringly growing. Therefore, various mushroom strain culture techniques are now refined agriculture. One of the most important technologies in the food and beverage industry. At present, mushroom products and edible fungi products that are generally seen require very good varieties or varieties 'and the combination of culture techniques'. However, due to the steps and production of strain culture techniques The methods are different, so whether the characteristics of the strain itself is a better microbial strain, whether it has better culture technology and effectiveness, and whether it is cost-saving has become the most important issue for developers; therefore, how to develop a The efficacy of the new mushroom microbes and cultivation and production technology has long become a developer and system 1280278. The manufacturer wants to overcome the __ question, and the topic presented in the social content is also the motivation of the inventor to develop the case. In general, the most common method of cultivating and cultivating at present is to use the space bag to artificially sterilize the production method. From (4) to the production method, the human Guardian and the younger mouth have passed the word, and there has not been a secretive Xingcheng New Zealand model. The owner of the 雠 雠 杏 杏 杏 杏 杏 杏 杏 杏 杏 杏 杏 杏 杏 杏 杏 杏 杏 杏 杏 杏 杏 杏 杏 杏 杏 杏 杏 杏 杏 杏 杏 杏 杏 杏 杏 杏 杏 杏 杏 杏 杏 杏 杏 杏 杏 杏 杏As a result, the cost of each manpower is not enough. The second is that because the traditional producers create the best urban environment, the products produced by each batch of apricot legs will have a unique yield, sputum, and even a bitter taste, which cannot be achieved. In response to the above shortcomings, in the pursuit of ideals, consumption and progress, we are looking forward to the goal of improvement. - The most common Apricot Bowei (scientific name, he (four) plus town sleeves (DC. .Fr.) Quel)) microbes such as · ATCC 36G47 and HOLLAND 150 two strains 'four (ATCC 36047) strains of raw growth The territory is Czechoslovakia in Eastern Europe, and the latter (HOLLAND150) county is unknown (if necessary, it can be inquired). The above two strains are characterized by the characteristics of the mushrooms observed by the mixture of sawdust and rice bran. The horticultural condition is as follows (eye-and tea reading accessories), and the following contents also reveal two common 1280278 known apricot strains The existing problems: Following the Chen _ Naqu 15__, under the conditions of general cultivation, 'there is not a neat record, it is easy to produce too many fruiting bodies, so that the New Zealand is relatively small and so on; unless the _ _ _ _ straight in a very fluent situation Or continue to use the centrifugal sprayer to add fish, causing the presence of water droplets on the surface of the mushroom umbrella: otherwise the leg 7g_ umbrella surface silkworm tumor appears, but occasionally there is a darker stitching, the ··47 hiding microbes _ In particular, the stipe in the knife is specially 'i Jian only, the negative ground is very delicate, the taste is very good, the low temperature storage, the shelf storage life is long, but the yield and the efficiency are lower than the L_(10), the strain is caused by the Erwinia bacteria. Soft rot and Cobweb disease caused by gamma secrets (10) are not disease-resistant. HOLLAND 150 strain: The mushroom is faster than ATCC 36〇47. Under the general cultivation and management conditions, it has the advantage of being neatly tidy. It is not easy to produce k-spot fruit body than ATCC 36〇47 strain, so its 4 body is generally medium to Large, when cultivated under the same environmental conditions as Atcc 36047 strain, the surface of the umbrella of H〇LLAND 15〇 strain is more likely to produce squamous tumors, resulting in poor appearance of the mushroom body, and the fungus umbrella of H〇LLAND 15〇 strain is deeper. Color and fragile, and the HOLLAND 150's microbial tissue is relatively soft (including bacteria umbrella and stipe), is not resistant to low temperature storage, shelf storage life is short, but the texture of the H〇LLAND 15〇il strain is very delicate. The mushroom body is relatively large, and its yield and mushroom efficiency sentence are higher than that of the other strain (ATCC 36047). This strain also recognizes the arachnoid 1280278 (Cobweb disease) which is caused by the bacteria Erwinia and is recognized by Cladobotryum dendroides. No disease resistance. In view of this, the inventor of this case examined and detailed the experience, and accumulated years of experience in the research and development of various species of strains and related strains, and finally developed a fast growth, good quality, out The mushroom is easy, the finished product is neat, the yield is high, and the material efficiency is high. The green _ gamma microorganism, and the unique cultivation method of the invention can give g silk growth speed, money material, neat product, high yield and biological efficiency. High edible microbes of Pleurotus eryngii. SUMMARY OF THE INVENTION The main object of the present invention is to provide a food-breeding organism that is fast-growing and has good quality, and the invention is systemized, cultivated in the environment and precise hybrid double (four) culture technology. The food of the apricot Baud, which grows with excellent hybrid dinuclear body, 'the invention is __ breeding method, and it is better to use U-bio' to make the apricot abalone grow faster, the harvest is full and coarse, and the tissue is solid and fine. The taste of the food is elastic. A secondary object of the present invention is to provide a method for producing apricot aphid culture which is easy to obtain, neatly finished, and high in productivity. The present invention utilizes (1) a mother seed culture step, (2) a cultivation wood step, and (3) cultivation. Material mixing and bottling steps; 1280278 (4). Sterilization and inoculation steps of cultivation materials; (5) Steps to take out, (7). Harvesting and packaging steps to stimulate better quality food names, and can produce finished products neatly , and w^^ biased out #speed, not only makes the mushroom 3〇%). The rate of accommodating (the amount of transcripts is greatly increased to Dafa: the provided food microbial cultivation technology, the cultivation and production side, the cultivation of apricots and edible micro-organisms) (1)·Cultivation, reproduction and preservation of hybrid parental species: hybridization= = and 胍 _ 150 _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ The powdered head, 20: 20 dry weight ratio, too rice sugar: Xiao used the mountain page hemp or other non-hardwoods, the marriage carbon _ yang value between 6.5 to 7. 〇, and water injury 3里I 利 3~6 5%; the cultivation strain uses the cultivating base used by the wood cultivator _ square wood chips: rice bran: powder head = 50 · · 15 : 35 dry weight ratio, wood chips can use mountain κ , maple or other wood species of broad-leaved tree species or their mixed 9 1280278, the rest of the production process and the original species; the field cultivation with "fairly, can be opened and peeled" after peeling cultivation (four) species transfer pure Room, the temperature of the city room is adjusted at 15~16 °C; finally, the collection of the spores is carried out. (3). Separation of monospora and monospora Confirmation and culture of strains • Separation of single spores by spore dilution culture separation method, after 2 to 5 days of constant temperature culture for 4 to 5 days, the medium containing the separate agar can be cut and placed in a test medium at 25 ° C. (4)·Confirmation, culture and preservation of the hybridization of the single spore@plant and the hybrid dinuclear. The obtained single spore g strain was propagated in the PDAS tube, and the single parent strain of the parental parent was separated by 0.5. Simultaneously inoculated on the same test medium at a distance of 1 cm, and placed in a 25 C fixed temperature culture for 25 to 30 days, the two g-falls merged with each other and lost their individuality, respectively, at the two g-fall fusion or the micro-mirror Microscopic examination confirmed that the silk body was produced from the silk. The silkworms of the hybrid X were transferred to the new medium and transplanted and stored in the same way as described above. (5)·The first cultivation of the hybrid dinucleate was screened: The confirmed hybrid dinuclear body is first made into the wood chip original species, and then made into the cultivation strain; the yield reduction characteristics from different dinuclear bodies are compared, the screening is more efficient, the appearance is good, the body structure is good, the money is early and neat. For further research - step planting test (6)·Re-cultivation of excellent hybrid dinuclear bodies and simple touch: From the first cultivation test 4, the dinuclear body of Weidunyi, and then the mechanical cultivation (four) species, select 10 1280278 and then expand the cultivation scale to obtain the most The superior Pleurotus eryngii has a hybrid dinucleus with high characteristics and high economic value of cultivation, and is compared with the hybrid parent or the current commercial cultivar to compare the edible microbes. 2. The edible microbial cultivation method of Pleurotus eryngii utilizes the following seven Main steps ^ Please refer to the figure - the edible microbial cultivation production method according to the present invention includes the following manufacturing steps: (1) · mother seed culture step; (2) · cultivation wood chip screening step (3) · Mixing and bottling steps of cultivation materials; (4) · Sterilization and inoculation steps of cultivation materials; (5) · Culture step of bacteria; (6) · Stimulation of mushrooming steps; (7) · Harvesting and packaging Steps; using the above 7 steps, can increase the yield, and with the (6) · stimulate the mushroom step 'can effectively control the number of mushrooms produced in each cultivation bottle to ensure the stability of production quality and production . [Embodiment] The detailed embodiments and other functions of the present invention in order to achieve the above objects and techniques, and a preferred embodiment are described in detail with reference to the drawings.
當可由之得一深入而具體 所示’本發明詳盡的說明When it is possible to get a deeper and more specific description, the detailed description of the present invention
:雜交親本ATCC t定溫箱再作保存。 薯葡萄醣洋菜培養基（PDA) 溫培養約1個月後，移入5 2 )·進仃雜父親本之雜製作及栽培菌種之製作與栽培：: The hybrid parental ATCC t thermostat is stored again. Potato Glucose Agaric Medium (PDA) After about 1 month of warm culture, transfer to 5 2)·Into the noisy father's miscellaneous production and cultivation and cultivation:
硬木類之闊#樹木屑，以碳酸_整嫌於β·5至7』之間，而水 份含量調整於6 3〜6 5%。以上材料_混合機充份混合後， 以全自動裝瓶機裝瓶、打洞及封蓋，利用高壓蒸氣殺菌。所使用 之塑膠瓶為llOOcc之ΡΡ塑膠瓶。在培養基冷卻後，可取在洋菜培 養基之菌絲洋菜塊或先製取麥粒菌種後接種，經18· 5〜20· 5°C培 養3 0〜3 5天，菌絲長滿基質後，即得杏鮑菇原種。 b ·栽培用菌種之製作··採用木屑菌種，所使用之栽培基質 配方為木屑··米糠··粉頭= 50:15:35之乾重比，木屑可使用山黃 麻、楓木或其他非硬木類之闊葉樹種木屑或其混合物，其餘製作 12 1280278 過域原種相同，接種時可利用以上所製作之原種做自動化接 種’接種完成後以1 8〜2 l°C定溫培養35至38天後，即得栽培 用菌種。 > C ·栽培㈣種之栽培：栽培㈣種製得後，即可開蓋檢查 有無雜菌污染，選取無雜L之栽_菌種，以人工做去皮處 理（菌搔）’即利用鐵製小勺挖除瓶口部份之舊原種及部份含菌絲 之新栽培基質之猶。去皮後*直接喷水緖。去皮後之栽培用 菌種移入城室内之軸上，此時之錢室之溫度調整於i 5〜 1 ’利㈣度控㈣與超音波加職調整相義度於9 〇〜 9 5%，此階段不須照明。 # d.馳子之轉：做孢子狀子倾先在無塵無 菌過遽箱内以乾淨的手或殺菌過之鑷子剖去神表皮，並利用利 :切除菌柄’以8_下插在放在殺®過之大型培養皿之鐵環架 突出之鐵絲上，其下置—殺菌過之濾紙，供孢子掉落之用。以上 衣置移入16(：疋溫箱，隔—或兩夜後，當擔孢子有相當量掉落於 濾紙時’在無菌無塵過濾箱内，以接種針小心到下擔孢子粉，並 裝入殺菌過之小麵顿如，移人叱定溫祕存備用。 (3 ).進行馳子之單孢分離及單孢祕之確認與培養： 採則包子稀釋培養分離法分離單孢，即將收集保存之雜交親 本ΰ株之觀水稀釋至適當濃度後，均勾塗佈於之 13 1280278 ^蚕皿之PM培魏上’經坑定溫培養紐天後，峨微鏡檢查 擔孢子發讀形。當-個視野僅相單獨—個孢子發芽形之小菌 洛，並能碟認無其他孢子或菌落在該視野周圍時，在相同視野内 以特製之婦辦心嶋德，並以接種 針小心移出含以上8落之洋菜塊，胁試管培養基略C定溫培 養。當移嫩嶋_搞繼，蝴峨以棉藍 =〇η _)染色後再運用普通顯微檢查菌絲上有無扣子體，若 有則非單孢g株’若無扣子體，㈣絲在”錢管上 正常，不呈粉狀生長或無扇狀生長時，應是單孢菌株。、又 (4)·進行單孢菌株間之雜交與雜交雙核體之確認 親存：獲得之單闕株經繁殖於PD A雜後，兩雜交 痒孢菌株以相隔〇· 5至1公分距離同時接種於同-試管 二定溫培養2 5至3〇天後，—^ 性，而若兩菌落相互融合且均失去其健 最下 .一减合錢_紅最上方及 取出囷絲鏡檢，取出之菌絲若有扣子體 成功，此時可將雜交成功之菌絲體移出培養於·私 述方法做定期移植及保存。 彳。養基’亚以河 14 1280278 上〇).進行雜交雙核體之首次栽培出兹篩選：經破認之雜 父雙核體，先製作其木城麥粒原種，·作栽·菌種，每雙 核體各接種三框，每框u瓶m之pp瓣瓶，所使用之栽培基 貝配方與栽培親本之配方相同，但去皮方式改為在細中央以直 徑1.8公分，一端為平整之鋁棒打深約丨至15公分之小洞即可， 以減少子實生過多之機會；從不暖核體比較，轉產兹效 率高、兹料觀佳、碰組織結實度良好、減早而整齊者，供 做再進一步栽培試驗比較。 (6) ·進行優異雜交魏體之賊培及出鄕性調查··從 I次栽培試驗篩麵得較優異之雙㈣，魏作成栽顧菌種， 母雙核體各栽培10框，以每框為—單位進行純早晚、錢整齊 度雜特性、產量與產兹效率之比較，以選取具有特色與栽培 二濟知值N之雜父雙核體’再擴大栽培規模，多次與雜交親本或 目前商業栽培品種做比較，以獲得最優異之杏鮑兹之食用微生 物’藉由上述步驟的搭配運作，本發明不僅可以讓杏_生產產 量大幅增加達，且少用人工、降低生產成本，並能提高杏鮑 4生產品質，使細制絲豐滿粗A、微生物堅實自細、食用 口感具彈性’故本發明確實是—種最實㈣杏雜方法及其 育成之食祕生物，並可以促進精緻農業的發展者。 15 1280278 值得-提的是，在進行雜交親本原種之培養繁歹直及保存時， 為期菌種之永久保存與遺傳特性之穩定可#，可採職氮保存方 式，並應利用程式降溫控制儀，將欲保存之杏顧菌種 降低代之速度從室溫降至—做後，急降至，。c，再立即投 入液見内保存’料，在進行雜交親柄種之培養繁殖及保存步 驟欲做液_存之_可先轉於殺菌過之小細，蚊溫培養 ，天後，存於無塵無菌過龍内小心移人㈣過之耐超低溫玻璃 安航(1.2ml，bQrQsiiiGateM_瓶⑽内。 同樣在使用*瓶4，封瓶後應確認有無密封，否則容易發生 ^爆炸。随猶_，侧％之撕溶液做财 保_。菌種取出解;東時，可直接將從液氮桶取出之菌種安瓶投 入37C至38t之溫柄解;東’ g__移人pDA培養即可； 當進行雜域权製作及細_之製作域贿，當紐 ’而擔孢子尚未產生前，為避免其他杏鲍兹子實 予^^__馳子之混合污染，可選取卿佳之栽培瓶 衣处理H賊成熟至s褶發育良好時，魏供抛子 收隼之用·p i 门，—方面’進行菌株之栽培及擔孢子收錢作時，不 ^口取好在不同出兹室單獨栽培，且不要同一 厂堅過滤箱内操作，谢娜馳伽败機會。— 16 1280278 本發明裁培管理之方式與栽 查出料晚，純財_ 株_ ;在祕後應調 等，待m έ正月a小姑多少、菌柄粗細與組織結實情形 長短=f 查物觀，包鳩表面顏色、粗細、 團:等、且？二度’菌傘大小、顏色、表面有無長疣狀瘤或菌絲 鬼專，亚硯察姑體有無萎黃現象及紀錄每絲體重量，子實體 2其Γ計料雙健之生物效率（生物效細鋪鮮重除以 本口土貝在接種時之乾重所得之百分比）。 二、杏鮑財成之食職生物栽培生產方法： ⑴·母觀養步驟：將本發明之紐M g種定期移植至 ::箸葡_培養基(其配方為馬鈐著2〇〇克、葡萄糖2 ,❹2 Q克及論水1公升），接著在無_作台上將培 養於馬鈴薯_财菜培養勒㈣鮑闕絲塊接種於經高壓蒸 汽殺菌過的織木顧培基㈣（其财為木賴麵體積比^ 0」，含水量調整為6 5%)，再以24〜肌的溫度培養至杏 編囷絲長滿細基質為止，至此乃制杏縣母種。 ^ (2)·栽培用木屑篩選步驟：以非硬木類闊葉樹新木屑（單 樹種或混合樹種皆可），_量灑水後，每隔二十天至—個月翻 1一次’經堆積二至六個月後，即可供製作栽培菌種之用。經堆 積過之木屑’以翻堆機移人電動木屑篩選機内，去除—些混雜於 17 1280278 木屑内之小型木塊、樹皮或其他雜物，然後將乾淨木屑以輸送帶 輸入木屑儲存槽或直接輸入木屑混合機之混合糟。 (3 )·栽培材料混合及織步驟··將乾淨木屑、粉頭及米 糠各別以輸送帶輸送至混合機内，依木屑、粉頭、米糖之乾重比 50 · 35 · 15的比率混合，另加入乾重比丄％之碳酸約調整混合物 的酸驗值，並加水將混合物之含水量調整於約65%左右。以上各 材料經過充分混合後，以混合機另—側之輸送帶將基質材料輸入 自動装瓶機，經自動化裝瓶，打洞、封蓋。 、（4)·栽培材料之殺菌及接種步驟：將裝填好基質材料之 •— ’瓶放入杀又菌爸後’先將殺菌爸之門關緊，隨即將鋼爐產生之 「、氣以人ji直接或㈣齡式自動控糊輸人殺菌勘，將溫度 逐漸升至1 2 rc，並維持45〜6〇分鐘後，停止加蒸氣，繼續保 持卻〜3G分鐘，即可將轴蒸氣漸轉出，待轴壓力降至零時， 即可開Η，將殺過菌之木屑轉瓶移人冷卻室冷卻，並打開紫外 =菌燈麵。當_基質降至常溫後(通«隔日），即可以輸 ===培材料錢人翻室，並以自動化麵機將錢益母種 ^出疋里接鶴栽培材料納。值得—提的是，接 ⑽、牆魏天紐及輸送帶清洗乾淨，並將自動接種機 及接種至之紫外線燈之開關打開至次日早晨關上。至於供做母 18 !28〇278 用之菌種瓶及裝菌種瓶之塑 菌箱之和你a L ，、表面應在接種室外之無 任他Γ 酒精擦拭乾淨，並徹底小錢視_瓶内料 :何_或是否有菌絲生長異常之現象，如有任何可疑=有均' …放茱使用。當母種之菌種瓶搬 且: 帅 m 文低主傻接種别宜再以酒精 室前 ，以減少雜菌污染。 應在接種室鱗之顿室財手術用衣帽 二拭其表面’再行使用。接種室内之操作人員在進入接種 .浦培養步驟··該栽培材料瓶㈣麟母種接種後 f為菌種瓶’菌種瓶再以輸送帶移送至菌種培養室，在2Q_23t：的 ^境下培養35-40天，8觀養室在叙g她培麵應先徹底打 喻r亚予以消毒，以避免_或紅鱗因連續培養而累積其族 群’因而f彡響啦，賊g種培養的成功率。 拼(6).刺激出兹步驟：杏飽兹菌絲長滿菌種瓶内的栽培基 *質後’先開蓋檢麵内基質有絲g，並選取無任何雜菌污染之 菌種’以自動去皮機打洞’在瓶口中央以直徑18公分，打深約工 紅5公分之小洞即可’以減少子實體產生過多之機會；不加水加 蓋，移入出菇室，室内溫度調整於16〜18t，利用超音波喷霧器 以定時器調整溼度於86, ％，室内二氧化礙濃度控制在⑽〇ppm 以下。 19 1280278 (7 ).採收及包裝步驟：杏鲍細體長至適宜採收之發育 階段’通常為菌傘邊緣仍未反捲，採收時宜將自瓶口長出之兹體 整把握住，先左右略微搖動後，再向上拔起杏鮑紐體。採收後 整把或單支杏鮑絲部附著之木屑以刀片切除，再將杏飽兹之兹 體一-分開，並依兹體大小分級包裝；以上所述即為本發明栽培 方法的特徵内容。 另在該栽培用_之栽培時，當打洞後之瓶口表面出現透明 水滴時，即表示即將出菇，此時可將出菇室相對溼度降低至 85 9(U ’-直至採收結束為止’可減少細菌病之發生與避免形成 過多之子實體’影響杏鮑菇品質等級。 再請參閱第二圖、第三韻、第三_2圖及第三_3圖，第三^ 圖係本發明ARI-ERYN-1跟ATCC 36047及HOLLAND 150兩品種的生長 特性比較圖表，第三-2圖係本發明ari-eryn-i跟ATCC 36〇47及 HOLLAND 150兩品種的子實體比較圖表，第三圖係本發明 ARI-ERYN-1跟ATCC 36047及HOLLAND 150兩品種的出菇特性比較圖 表。 其中揭露本發明創作的杏鮑菇育成之食用微生物，其主要係 藉由杏鮑菇（學名^取/7· (Dc· :Fr·) Qu6l·)内雜 交親本之ATCC 36047及HOLLAND 150兩菌株培養出的擔孢子之 20 1280278 單抱菌株進㈣翅合，纽雙铺者，制學特㈣絲生長溫 度5IC、細7〇〜1251财挪财75删的齡細内，如寄 存編號謂纖7 ;另由第：圖外觀可知，本發财祕育成之 食用微生物外觀具有俯視近圓型或扇型、傘緣内f的特性，且全 緣有時呈波浪狀，傘肉厚，菌柄側生或偏生，偶見令生。 綜上所述，本發明係依據杏驢育成之食用微生物及其栽培 生產方法，後者包括··⑴.母種培養步驟；⑵栽培用木屑筛選 步驟；（3).栽培材料混合及瓶裝步驟；⑷栽培材料之殺菌及接 種步驟’（5).菌種培養步驟；⑹刺激出益步驟，·⑺.採收及包 裝步驟等七個步驟，藉由上述的製造步驟不僅可以解決以錄生 :培養的_，而且其育叙仙微生物可以達成生產快速、提 昇艮用口感及提供|父佳產品品質的多種功效，故本發明確實是一 種最實用的杏鮑兹育成之食用微生物及其栽培生產方法；所以， 本發明之『具有錢之可伽性』應已讀置疑，此外，本案發 明實施例所揭露之構造，在申請之前並未曾見於諸刊物，亦未曾 被么開使用，不但具有如上功效增進之事實，更具有提昇多項杏 鮑兹原本食用品質的附加功效，是故，本發明之『新穎性』以及 進ッit』都均已符合專利法規，妥依法提出發明專利之申請， 祈請惠予審查並早日賜准專利，實感德便。 21 1280278 【圖式簡單說明】 第一圖係本發明之步驟圖。 第二圖係本發明杏鮑菇育成之食用微生物外觀。 第三-1 圖係本發明ARI-ERYN-1 跟ATCC 36047及HOLLAND 150兩 品種的生長特性比較圖表一。 第三-2圖係本發明ARI-ERYN-1 跟ATCC 36047及HOLLAND 150 兩品種的子實體比較圖表二。 第三-3圖係本發明ARI—ERYN—；^RAT(X 36〇47及HOLLAND 150 兩口口種的出兹特性比較圖表三。 附件部份 附件一本發明栽培方法運作之圖片。 22The width of the hardwood class #树屑, with carbonic acid _ allegedly between β·5 to 7′′, and the water content is adjusted to 6 3~6 5%. After the above materials_mixer is fully mixed, it is bottled, punched and sealed with a fully automatic bottling machine and sterilized by high pressure steam. The plastic bottle used is a plastic bottle of llOOcc. After the medium is cooled, it can be inoculated in the hyphae of the acacia medium or after the wheat germs are firstly prepared, and cultured at 18·5~20·5°C for 30 to 3 days, the mycelium is overgrown with the substrate. After that, the original species of Pleurotus eryngii is obtained. b ·Preparation of cultivation strains ··Use of woody fungus species, the cultivation substrate formula used is wood chips · rice bran · powder head = 50:15:35 dry weight ratio, wood chips can use mountain jute, maple Or other non-hardwood broad-leaved tree species wood chips or mixtures thereof, the rest of the production of 12 1280278 is the same as the original species. When inoculation, the above-made original seeds can be used for automatic inoculation. After inoculation, the cells are cultured at a temperature of 1 8~2 l °C. After 38 days, the cultivation strain is obtained. > C · Cultivation (four) species cultivation: After cultivation (four) of the species, you can open the lid to check for the contamination of the bacteria, select the plant without the miscellaneous L, and use the artificial peeling treatment (bacteria) The iron spoon removes the old stock of the bottle part and some of the new cultivation base containing the hyphae. After peeling, * directly spray the water. After the peeling, the cultivation strain is moved into the axis of the city indoors. At this time, the temperature of the money room is adjusted to i 5~1 'Li (4) degree control (4) and the ultrasonic adjustment is adjusted to 9 〇~ 9 5% There is no need for lighting at this stage. #d. Chizi's turn: Do spores and dump the skin in the dust-free sterile container with a clean hand or sterilized scorpion, and use the profit: remove the stipe 'to insert it under 8_ On the iron wire protruding from the iron ring frame of the large Petri dish, the sterilized filter paper is placed underneath for the spore drop. The above clothes are moved into the 16 (: 疋 箱, compartment, or after two nights, when the spores are dropped in the filter paper in the sterile dust-free filter box, carefully inoculated with the inoculation needle to the lower spore powder, and loaded Into the sterilized small noodles, such as, transfer the sputum to the temperature of the secret reserve. (3). The separation of single spores and the confirmation and culture of the single spores: the separation of the single spores by the dip-culture separation method After collecting and preserving the diluted parental plant, the water is diluted to the appropriate concentration, and then applied to the 13 1280278 ^PM of the Pei Wei on the 'Pit stagnation temperature to culture New Zealand, 峨 microscopic examination of the spores Read the shape. When a field of view is only separate - a spore germination of the small fungus, and can discard no other spores or colonies around the field of view, in the same field of vision with a special woman to do the heart, and The inoculation needle carefully removes the above-mentioned 8 falling amaranth pieces, and the test tube medium is slightly C-temperature cultured. When the transplanting is done, the butterfly is dyed with cotton blue=〇η_) and then the ordinary microscopic hyphae are used. There is no button body on the body, if there is a non-single spore strain, 'if there is no button body, (4) silk is normal on the money tube When it is not in powdery growth or in the absence of fan-like growth, it should be a single spore strain. (4) Perform hybridization between the single spore strains and confirm the hybridization of the dinuclear: the obtained single plant is propagated to PD A. After the miscellaneous, the two hybrid echinococcosis strains were simultaneously inoculated at the same distance of 5 to 1 cm from the same-test tube two fixed temperature culture for 25 to 3 days, and then the two colonies merged and lost their health. The lowest. One reduction of money _ red at the top of the red and take out the silk examination, if the hyphae taken out is successful, the successful hybrid mycelium can be removed and cultured in a private method for regular transplantation and preservation.彳. Yangji 'Ya Yihe 14 1280278 Shangyu). The first cultivation of the hybrid dinuclear is screened: the duplicate father's dinuclear body, the first grain of the wood grain, the planting strain, Each pair of nucleus is inoculated with three boxes, each of which has a pp-valve bottle of m bottle, and the cultivating basal formula used is the same as that of the cultivating parent, but the peeling method is changed to a diameter of 1.8 cm in the center of the center, and one end is flat. The aluminum rod can be drilled to a hole of about 15 cm to reduce the number of sub-living machines. Compared with the non-warm nuclear body, the conversion efficiency is high, the material is good, the tissue is good, and the early and neat is used for further cultivation experiments. (6) · Performing excellent hybrids鄕 调查 · · · · · · · · · · · · · · · · · · · · · · · · · · · · · · · · · · · · · · · · · · · · · · · · · Comparing the yield with the efficiency of the production, the selection of the dual-nuclear body with the characteristics and cultivation of the Nichichi N is re-expanded, and it is compared with the hybrid parent or the current commercial cultivar to obtain the best apricot. The edible microbe of Bautz's operation by the above steps, the invention can not only increase the production yield of apricot _, but also use less labor, reduce the production cost, and improve the production quality of apricot 4, so that the fine silk is full. The crude A, the microorganisms are solid and fine, and the taste of the food is elastic. Therefore, the present invention is indeed the most practical (four) apricot hybrid method and the secreted food secreted organism thereof, and can promote the development of refined agriculture. 15 1280278 It is worth mentioning that, in the cultivation and breeding of the hybrid parental species, the permanent preservation of the species and the stability of the genetic characteristics can be used, and the nitrogen storage method can be adopted, and the program should be cooled by the program. The instrument will reduce the speed of the apricot species to be preserved from room temperature to - afterwards, it will drop sharply. c, immediately put in the liquid to see the inside of the 'material, in the breeding and breeding of the pro-handling species and the preservation steps to make the liquid _ deposit _ can be transferred to the sterilized small fine, mosquito temperature culture, after the day, stored in Dust-free sterile dragons carefully moved (4) to the ultra-low temperature glass Anhang (1.2ml, bQrQsiiiGateM_ bottle (10). Also in the use of * bottle 4, after sealing the bottle should be confirmed whether there is a seal, otherwise it is prone to ^ explosion. With the Jude _, the side% of the tear solution to do the fortune _. The strain is taken out; in the east, the strain can be directly taken from the liquid nitrogen barrel into the 37C to 38t temperature handle solution; East 'g__ transfer human pDA culture It can be used; when the production of miscellaneous rights and the production of bribes, when the New Zealand and the spores have not yet been produced, in order to avoid the mixed pollution of other apricots, ^^__Chi, you can choose Qing Jiazhi When the cultivation bottle is used to treat the mature spleen to the spleen, the wei pleats are used for the spleen, and the pi door is used for the cultivation of the strains and the cultivation of the stalks. The room is cultivated separately, and do not operate in the same factory's filter box, Xie Nachi gambling chance. — 16 1280278 The method of cutting and cultivating management is late with planting materials, pure wealth _ strain _; should be adjusted after the secret, wait for m έ 月 a a a small number of aunt, the length of the stipe and the length of the tissue sturdy = f inspection view, the surface of the package Color, thickness, group: etc., and the second degree 'bacteria umbrella size, color, surface with long sputum tumor or mycelial ghost, Acha gu detection of chlorosis and record the weight of each body, fruiting body 2 The bio-efficiency of the biotechnical material (the percentage of bio-efficiency and fine-grained fresh weight divided by the dry weight of the local shellfish at the time of inoculation). II. Apricot Baocaicheng's food production and cultivation methods: (1)·Female observation Step: The graft of the present invention is periodically transplanted to:: 箸 _ _ medium (the formula is 2 gram of horse 、, glucose 2, ❹ 2 Q grams and 1 liter of water), and then in the absence of _ The cultivar was cultured in potato _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ It is cultivated at a temperature of 24~ muscle until the apricot silk is covered with a fine matrix, and is the mother of the apricot. ^ (2)·Cultivation Sawdust screening step: new wood chips (single tree species or mixed tree species) of non-hardwood broad-leaved trees, _ after sprinkling water, turn every 20 days to - month, 'after two to six months of accumulation, ie It can be used for the cultivation of cultivated species. The accumulated wood chips are removed from the electric sawdust screening machine by the stacker to remove some small wooden blocks, bark or other debris mixed in the wood chips of 17 1280278, and then the wood chips are cleaned. The conveyor belt is input into the wood chip storage tank or directly into the mixed mixture of the wood chip mixer. (3)·Cultivation material mixing and weaving steps··The clean wood chips, powder head and rice bran are transported separately to the mixer by the conveyor belt, according to the wood chips, The dry weight of the powder head and the rice sugar is mixed at a ratio of 50 · 35 · 15 , and the acid value of the mixture of the dry weight ratio 丄 % is added, and the water content of the mixture is adjusted to about 65% by adding water. After the above materials are thoroughly mixed, the matrix material is fed into the automatic bottling machine by the conveyor belt on the other side of the mixer, and the bottle is automatically bottled, punched and sealed. (4) · Sterilization and inoculation steps of the cultivation material: The material will be filled with the matrix material - 'The bottle is put into the killing and the bacteria dad' first close the door of the sterilization dad, and then the steel furnace produces the ", the gas is human Ji direct or (four) age-type automatic control paste input and sterilization, the temperature is gradually increased to 12 rc, and after maintaining for 45~6 〇 minutes, stop adding steam, continue to maintain but ~ 3G minutes, the shaft vapor can be gradually turned When the pressure of the shaft is reduced to zero, the smashed wood chips can be transferred to the cooling chamber for cooling, and the UV-bacteria lamp surface is turned on. When the _ matrix is lowered to normal temperature (passing the next day), That is to say, you can lose === training materials to turn the room, and use the automatic noodle machine to take the money to the mother plant to pick up the crane cultivation materials. It is worth mentioning that, pick up (10), wall Wei Tianxin and conveyor belt cleaning Clean, and open the switch of the automatic inoculation machine and the ultraviolet light inoculated until the next morning. As for the mother bottle of 18!28〇278 and the plastic bottle of the strained bottle, you and L , the surface should be in the outside of the vaccination, the alcohol is wiped clean, and completely small money _ bottle contents: He _ or There is a phenomenon of abnormal growth of mycelium, if there is any suspicious = there is a ... ... use. When the mother of the species of the bottle moved and: handsome m Wen low main silly inoculation should not be used in front of the alcohol room to reduce bacteria Contamination. In the inoculation room, the room should be used for the operation of the coat and the second surface of the room. The operator in the inoculation room is entering the inoculation. The step of culturing the culture material bottle (4) after the inoculation of the seedlings is The strain bottle of the strain bottle is transferred to the culture chamber of the strain by the conveyor belt, and cultured for 35-40 days in the environment of 2Q_23t: 8. The observation room should be thoroughly disinfected by the sub-culture. In order to avoid _ or red scales accumulating their ethnic groups due to continuous cultivation, thus f rang, the success rate of thief g culture. Fighting (6). Stimulating the steps: apricot full of hyphae overgrown in the bottle After the cultivation base* quality, the first step is to open the cover and check the surface of the substrate with silk g, and select the bacteria without any contaminated bacteria 'to open the hole with automatic peeling machine' in the center of the bottle mouth with a diameter of 18 cm, deep work Red 5 cm hole can be used to reduce the chance of excessive production of fruiting bodies; without water, cover, move into the mushroom room, indoor temperature Adjusted to 16~18t, use the ultrasonic atomizer to adjust the humidity to 86%, the indoor oxidation resistance is controlled below (10)〇ppm. 19 1280278 (7). Harvesting and packaging steps: apricot abalone The developmental stage that is suitable for harvesting is usually not the edge of the umbrella. The harvesting should be carried out from the mouth of the bottle. After shaking slightly, the abalone can be pulled up. After harvesting, the sawdust attached to the whole abalone or single apricot abalone is cut off by a blade, and then the apricot is fully separated and packaged according to the size of the body; the above is the characteristic of the cultivation method of the present invention. In addition, in the cultivation of the cultivation, when a transparent water droplet appears on the surface of the bottle mouth after the hole is punched, it means that the mushroom is about to be produced, and the relative humidity of the mushroom room can be lowered to 85 9 (U '- until mining). At the end of the harvest, it can reduce the occurrence of bacterial diseases and avoid the formation of excessive fruiting bodies' affecting the quality grade of Pleurotus eryngii. Please refer to the second figure, the third rhyme, the third _2 figure and the third _3 picture. The third picture is a comparison chart of the growth characteristics of the ARI-ERYN-1 and ATCC 36047 and HOLLAND 150 of the present invention. The 3-2 figure is a comparison chart of the ari-eryn-i of the present invention with the fruit bodies of the ATCC 36〇47 and HOLLAND 150, and the third figure is the mushroom of the ARI-ERYN-1 and the ATCC 36047 and HOLLAND 150 of the present invention. Feature comparison chart. The edible microbes bred by Pleurotus eryngii which are created by the present invention are mainly disclosed by ATCC 36047 and HOLLAND 150 of the hybrid parent of Pleurotus eryngii (scientific name: /7·(Dc· :Fr·) Qu6l·) 20s of the strains cultured by the strains 1 1280278 single-cage strains into the (four) wing, the double-double shop, the special (four) silk growth temperature 5IC, fine 7〇~1251, the fortune, the deletion of the age, such as the registration number Fiber 7 ; Another appearance: The appearance of the figure shows that the appearance of the edible microbes cultivated by the present financial secretary has the characteristics of a near-circular shape or a fan shape, and the inside of the umbrella edge, and the whole edge is sometimes wavy, and the umbrella is thick and the bacteria are thick. The stalk is lateral or partial, and occasionally occurs. In summary, the present invention is based on the edible microorganisms cultivated by apricot and its cultivation and production methods, the latter including: (1). mother seed culture step; (2) wood cutting step for cultivation; (3) cultivation material mixing and bottle filling steps (4) Sterilization and inoculation steps of the cultivation material '(5). Culture step; (6) Stimulation benefit step, · (7). Harvesting and packaging steps, etc., can be solved by the above-mentioned manufacturing steps : cultivating _, and its cultivating microorganisms can achieve a variety of functions such as rapid production, improved taste and quality of the product. Therefore, the present invention is indeed the most practical edible microorganism cultivated by apricot and its cultivation. The production method; therefore, the "having money" of the present invention should be questioned. In addition, the structure disclosed in the embodiment of the present invention has not been seen in publications before the application, nor has it been used. The fact that the above-mentioned effects are enhanced has the additional effect of improving the original eating quality of a plurality of apricots, and therefore, the novelty and the enthusiasm of the present invention have been met. In conjunction with the patent regulations, the application for invention patents shall be filed in accordance with the law, and the application for review and early grant of patents shall be prayed for. 21 1280278 [Simple description of the drawings] The first figure is a step diagram of the present invention. The second figure is the appearance of edible microorganisms bred by the Pleurotus eryngii of the present invention. The third-1 figure is a comparison chart of the growth characteristics of the ARI-ERYN-1 of the present invention and the ATCC 36047 and HOLLAND 150 varieties. The third-2 figure is a comparison of the ARI-ERYN-1 of the present invention with the fruit bodies of the two varieties ATCC 36047 and HOLLAND 150. The third to third figures are a comparison of the characteristics of the ARI-ERYN-;^RAT (X 36〇47 and HOLLAND 150) of the present invention. Figure 3. Annex A Annex Figure 1 shows the operation of the cultivation method of the present invention.