1271219 九、發明說明： 【發明所屬之技術領域】 本發明係有關於一種微流體(microfluidic)裝置，尤其 為關於一種具有特殊設計之腔體(chamber)的流體導流模組 (fluid flow conducting module)，其可導引不同的微流體進入 腔體。 【先前技術】 微流體技術廣泛應用在生化分析上，如微幫浦 (micro-pump)、微閥門（micro-valve)、微過濾器 (micro-filter)、微混合器（micro-mixer)、微管道 (micro-channel)、微感測器(micro-sensor)等元件。微流體技 術大多被使用於微晶片（micro-chip)上，以進行樣品前處 理、混合、傳輸、分離和偵測等程序。利用微流體晶片進 行生物醫學檢測或分析，具有節省人力與時間的優點。 美國專利文獻第6,073,482號揭露一種備有感測器的 微管道之流體裝置，藉由閥的開及閉，將三種不同的流體 即校正劑(calibrant)、緩衝劑(buffer)與分析劑(analyte)導入 微管道，當分析劑通過感測元件的點時，即可偵測分析劑 的存在或濃度，此流體裝置可做微型生化檢測。 6 .1271219 同樣地，美國專利文獻第6,073,482號揭露一種增強吸 收力之差動(differentia)萃取(extraction)裝置，如第一圖所 示，此Η形微流管道裝置之結構1〇〇可用來作為分離或萃 取某一種溶液(solution) 〇 在2001年’李國賓教授等人在“Micromachined Pre-focused lxN Flow Switches for Continuous SampleBACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a microfluidic device, and more particularly to a fluid flow conducting module having a specially designed chamber. ), which can direct different microfluidics into the cavity. [Prior Art] Microfluidic technology is widely used in biochemical analysis, such as micro-pump, micro-valve, micro-filter, micro-mixer, Micro-channel, micro-sensor and other components. Microfluidics are mostly used on micro-chips for pre-sample processing, mixing, transfer, separation, and detection. Biomedical testing or analysis using microfluidic wafers has the advantage of saving manpower and time. U.S. Patent No. 6,073,482 discloses a fluid device for a microchannel having a sensor. By opening and closing the valve, three different fluids, a calibrant, a buffer, and an analytical agent (analyte) are disclosed. The micro-duct is introduced, and when the analyte passes through the point of the sensing element, the presence or concentration of the analyte can be detected, and the fluid device can be used for micro biochemical detection. U.S. Patent No. 6,073, 482 discloses a differential absorption extraction device for enhancing absorption. As shown in the first figure, the structure of the circular microfluidic conduit device can be used. As a solution for separating or extracting a solution, in 2001, Professor Li Guobin and others at "Micromachined Pre-focused lxN Flow Switches for Continuous Sample
Injection，J· Micromech· Microeng·，11(2001)，ρρ· 567-573” 揭露一種微管道結構，藉由幫浦提供之流體動力，可將不 同樣品注入至不同之出口流道(outlet)，以連接不同的流體 分析晶片。 在 2002 年，Powers 等人在“A Microfabricated Array Bioreactor for Perfused 3D Liver Culture, Biotechnology and Bioengineering，78(2002)，pp. 257-269”揭露一種三維(3D)微 結構’利用連續週流的(continuous cyclic)方式，培養老鼠 肝臟細胞和觀察細胞的變化。 【發明内容】 本發明的主要目的是提供一種流體導流模組，此流體 導流模組對不同的微流體進入腔體時，提供動態導流之穩 定功能(stable function) 〇 7 .1271219 依此，此流體導流模組之一特性為，當腔體内之微流 體為層流(laminate-layer flow)狀態時，腔體内之流體的流線 幾乎互相平行。 此流體導流模組之另一特性為，設置數個出口流道， 精由調整不同流體間的流速比率(flow speed ratio)，將特定 的流體導引至一或數個特定出口。 此流體導流模組之另一特性為，藉由調整流體之流速 來動態調整流體流過此模組的時間。 此流體導流模組之另一特性為，具有廣泛的流速範 圍，結構簡單製作成本低，可應用於細胞培養、細胞對藥 物檢測或生化檢測等。 為利用上述特性和達成本發明之主要目的，本發明之 流體導流模組包含至少兩個入口流道(inlet)、數個出口流道 及一個内含第一檔塊(block)及第二檔塊之腔體。此腔體於 中央有一漸闊段(gradually wider section)，和一第一收斂端 (convergent end)及一第二收斂端。第一收斂端連接該入口 流道，第二收斂端連接該出口流道。此二收斂端有圓形的 8 1271219 邊緣。第一槽塊在其前端(in the front)係呈一銳角(acute angle)狀，並配置在接近於第一收敛端的收斂點(convergent point)。第二檔塊在其前端係呈一凸狀表面（conVex surface)，並配置在接近於第二收斂端的收斂點。 藉由此特殊設計及内含二擋塊之腔體，避免了流體發 生分離、減短流體需要完全發展(folly developed)的距離及 避免流線扭曲(distortion)，以減少流線成為直線的轉換區域 (transfer portion)。因流體是完全地發展，在出口流道處的 流速不會受入口流道流體參數的影響。此特殊設計之腔體 可藉由在出口流道處，以適當間距配置多個出口流道通 道，則在每一出口處可收集同類的流體。 此流體導流模組之導引過程包含(a)使用兩個或兩個 以上入口流道去導引一或數個注入腔體的流體，此腔體有 一第一標塊及一第二檔塊。第一檔塊在其前端係呈一銳角 狀’並配置在接近於第一收斂端的收斂點。第二檔塊在其 前端係呈一凸狀表面，並配置在接近於第二收斂端的收斂 點。（b)採用一個在中央有一漸闊段的腔體，且於此腔體 設置一第一收斂端及一第二收斂端。第一收斂端連接該入 口流道’第二收斂端連接該出口流道，此二收斂端有圓形 的邊緣。（c)藉由改變流體的流速來調整流體流過之區域。 9 1271219 (d) β又置一或多個出口流道於此腔體末端處，且導引流體 流向-或數個出α流道，以便收集流體。 根據本發明，此腔體之二收斂端的漸斂點為一圓形的 邊緣’使得腔體在巾央部分是寬的，而朝向人口流道與出 口流道之終端處的部分是窄的。流體經由入口流道注入腔 體’流過此腔體，且被導引流向一或數個出口，以便收集 • 和分析此流體。 茲配合下列圖示、實施例之詳細說明及申請專利範 圍’將上述及本發明之其他目的與優點詳述於後。 【實施方式】 第一圖為根據本發明之較佳實施例之流體導流模組的 _ 概要圖。參考第二圖，流體導流模組200包含兩個入口流 道201a與201b，兩個出口流道203a與203b及一個腔體 202。腔體202的中央有一漸闊段。此腔體202並備有一第 一收斂端204a及一第二收斂端204b。此外，腔體202有一 第一擋塊2021及一第二擋塊2022。腔體202開始收斂並朝 向收斂端的中間點(middle points)被定義為收斂點。第一收 斂端204a連接入口流道201a與201b，第二收斂端204b連 接該口流道203a與203b。第二圖中僅顯示兩個入口流道與 10 1271219 兩個出口流道’而入口流道與出口流道的數目可根據實際 應用的需要來改變。 如第二圖所示，第一擋塊2021的前端呈一銳角狀，且 此擋塊2021配置在此銳角朝向第一收斂端與入口流道之連 接點的收斂點。第二擋塊2022的前端呈一凸狀表面，並且 朝向第一擋塊2021 ’且此擋塊2022配置在朝向出口流道之 層流的收斂點。第一擋塊和第二擋塊使得當腔體内之流體 為層流狀態時，腔體内之流體的流線互相平行且幾乎成直 線。第二圖中所不的虛線為流體之流動樣式(flowpattern)。 藉由策略性地配置擋塊2021與擋塊2022，從入口流道2〇la 與201b來的流體以層流方式被導引流過腔體202。值得注 意的是，虛線在腔體202中央漸闊段的地方係平行的。 藉由此腔體的中央漸闊段和此二擋塊，當流體進入腔 體時，可避免流體發生分離、減短流體需要完全發展的距 離及避免流線扭曲，以減少流線成為直線的轉換區域。 當流體進入腔體後，在出口流道處的流速不會受入口 流道流體參數的影響，因為流體是完全地發展。此特殊設 計之腔體在每一出口處可收集同類的流體，此可藉由以適 η 1271219 δ間距配置適當的出口流道通道(〇utletchannel)來達成。 根據本發明，腔體202可為對稱(symmetric)腔體，當 腔體内之流體為層流狀態時，其流線大體上平行且幾乎成 直線。區塊2021之前面以對稱銳角三角形(acutesymmetric triangle)的形狀可更有利於導引流體。 本發明之流體導流模組的導引原則如下。此導流模組 使用兩個或兩個以上入口流道去導引注入腔體的流體，此 腔體具一中央漸闊段。經由此特殊結構的腔體，當腔體内 之流體為層流狀態時，流體的流線幾乎互相平行，且流體 之間不會互相混合。當來自入口流道之流體以相同流速注 入腔體後’將流過等容積的區域。另'一方面，當來自入口 流道之流體以不同流速注入腔體後，其流過的區域容積會 隨入口流速的比例而變化。藉由在腔體末端裝設一或數個 出口流道，可導引同類流體進入一或數個出口，以便收集 此流體。 綜上所述，流體導流模組的導引過程可歸納如下:(a)首 先，使用兩個或兩個以上入口流道去導引一或數個注入腔 體的流體。（b)其次，採用一腔體，其具有一第一擋塊、一 12 1271219 第二擋塊以及一中央漸闊段。其中此第一擋塊的前端呈一 銳角狀’第一塊的刖端呈一凸狀表面。依此，當腔體内 的流體為層流狀態時，此流體的流線幾乎互相平行。此腔 體並具有一第一收斂端及一第二收斂端。第一收斂端連接 該入口流道，第一收敛端連接該出口流道，此二收敛端有 圓形的邊緣。（C)然後，藉由改變流體之入口流速來調整流 體流過的區域。（d)最後，設置一或多個出口流道於此腔體 # 末端處，且導引流體朝向一或數個出口流道，以便收集此 流體。 此外，步驟(b)可以再包含一步驟，亦即，將第一擋塊 配置在接近於第一收斂端的收斂點，且其銳角朝向第一收 斂端與入口流道的連接點。 同樣地，步驟(d)可以再包含一步驟，亦即，將第二擋 塊配置在接近於第二收斂端的收斂點，且其凸狀表面朝向 第一擋塊。 本發明之流體導流模組的功能與流體的比重、黏度 (viscosity)、入口流速、微管道的管徑(diameter)、收敛段的 寬度(width)及腔體的形狀與大小有關。所以，可利用以上 13 1271219 因子(factor)作為此流體導流模組的控制參數。 第二a至第三e圖說明當進入入口流道的入口流速改 變時’形成不同的流體流動樣式。第三a至第三e圖中以兩 個入口流道為例，其一為頂部入口流道，另一為底部入口 流道。圖中的vl與V2分別代表頂部入口流道及底部入口 流道的入口流速。第三a至第三e圖中，¥1與¥2之間的比 值依序分別為1:1、1:2、1:3、1:4、及1:5。 如第三a至第三e圖所示，當底部入口流道的入口流 速高於頂部入口流道的入口流速時，虛線向上彎曲起來， 由底部入口流道的流體流過較大的區域。值得注意的是流 體也會照比例流向不同的出口流道。如圖所示，從底部入 口流道注入的流體被導引朝向整個底部出口流道和部分的 頂部出口流道。因此，本發明藉由調整入口流道之流體的 相對入口流速，可導引流體流過腔體内不同的區域，並朝 向不同的出口流道，以便收集和分析此流體。如先前所述， 入口流體的流速愈低，流過之區域的容積愈小。 如先前所述’本發明之其中一個優點為減短流體需要 完全發展的距離。因此’被不同流體流過的區域容積與佔 14 ,1271219 此腔體的剖面寬度(cross sectional Width)，其兩者之間的比 值與該不同流體的流速保持一種特殊的關係。 第四圖所示為本發明的-個應關，此例包含兩個入 口流道401a與401b、一個腔體202及五個出口流道4〇2a 至402e，其中腔體202含有一個三角形的擋塊2〇21及一個 圓形的擋塊2022。如第三a至第三e圖所示，藉由改變流 體的入口流速，第四圖中的流體可被導引至不同的出口流 道。以此設計，一種流體可被導引至多個出口流道，或是 不同的流體可被導引至相同的出口流道。對不同的應用， 入口流道與出口流道的數目可改變，並且相對的入口流速 可改變，以執行多樣化的檢測和分析，諸如材料的存在和 材料的濃度等。 • 與傳統的技術相較，本發明的流體導流模組提供了下 列的優點。（1)當流體為層流狀態時，其流線幾乎平行。(2) 藉由調整不同流體之間的流速比例，可導引特定的流體流 向一或多個特定的出口流道。(3)藉由調整流體的流速，可 動態調整流體流過此模組的時間。(4)此流體導流模組具有 廣泛的流速範圍，而結構簡單，使得廣泛應用時，其製作 成本低’這些應用範圍如細胞培養、細胞對藥物檢測或生 化檢測等。 15 1271219 惟，以上所述者，僅為本發明之較佳實施例而已，當 不能以此限定本發明實施之範圍。即大凡依本發明申請專 利範圍所作之均等變化與修飾，皆應仍屬本發明專利涵蓋 之範圍内。 、:[271219 【圖式簡單說明】 第一圖為一種傳統增強吸收力之差動萃取裝置其具有Η 形微流管道的結構。 第二圖為根據本發明之較佳實施例之流體導錢組的概要圖 第三a至第三e圖說明當進入入口流道的入口流迷改變時 形成不同的流體流動樣式。 第四圖為本發明之第二圖的一個應用例子。 【主要元件符號說明】 200 流體導流模組 201a、201b 入 口流道 203a、203b 出 口流道 202 腔體 204a 第一收斂端 204b 第二收斂端 2021 第一區塊 φ 2022 第二區塊 vl、v2入口流速 401a 入口流道 401b 入口流道 402a、402b、402c、402d、402e 出 口流道 17Injection, J. Micromech· Microeng·, 11 (2001), ρρ·567-573” discloses a micro-pipe structure in which different samples can be injected into different outlet channels by the fluid power provided by the pump. The wafers were analyzed by connecting different fluids. In 2002, Powers et al. disclosed a three-dimensional (3D) microstructure in "A Microfabricated Array Bioreactor for Perfused 3D Liver Culture, Biotechnology and Bioengineering, 78 (2002), pp. 257-269". 'Using a continuous cyclic method to culture mouse liver cells and observe changes in cells. SUMMARY OF THE INVENTION The main object of the present invention is to provide a fluid guiding module that is different for different microfluidic modules. When the fluid enters the cavity, it provides a stable function of the dynamic flow. 〇7.1271219 According to this, one of the characteristics of the fluid guiding module is that the microfluid in the cavity is laminar-layer flow. State of the art, the flow lines of the fluid in the cavity are almost parallel to each other. Another feature of the fluid guiding module is that several outlet channels are provided, and the different fluids are adjusted. The flow rate ratio is used to direct a specific fluid to one or several specific outlets. Another characteristic of the fluid guiding module is to dynamically adjust the fluid flow through the mold by adjusting the flow rate of the fluid. The other characteristics of the fluid guiding module are that it has a wide range of flow rates, simple structure and low manufacturing cost, and can be applied to cell culture, cell-to-drug detection or biochemical detection, etc. In order to utilize the above characteristics and achieve this. The main object of the invention is that the fluid guiding module of the present invention comprises at least two inlet inlets, a plurality of outlet flow channels, and a cavity containing the first block and the second block. The cavity has a gradually wider section at the center, and a first convergent end and a second convergence end. The first convergence end is connected to the inlet flow channel, and the second convergence end is connected to the outlet flow path. The two convergence ends have a circular edge of 8 1271219. The first groove block has an acute angle at its front end and is disposed at a convergent point close to the first convergence end. Second block The front end of the body has a convex surface (conVex surface) and is disposed at a convergence point close to the second convergence end. By the special design and the cavity containing the second stopper, the fluid separation is avoided and the fluid needs are shortened. The distance that is fully developed and avoids streamline distortion to reduce the transfer portion where the streamline becomes a straight line. Since the fluid is fully developed, the flow rate at the outlet runner is not affected by the inlet runner fluid parameters. This specially designed chamber can collect the same type of fluid at each outlet by arranging a plurality of outlet runner channels at appropriate intervals at the outlet runner. The guiding process of the fluid guiding module comprises (a) using two or more inlet flow channels to guide one or several fluids injected into the cavity, the cavity having a first block and a second block Piece. The first block has an acute angle at its front end and is disposed at a convergence point close to the first convergence end. The second block has a convex surface at its front end and is disposed at a convergence point close to the second convergence end. (b) A cavity having a tapered section at the center is provided, and the cavity is provided with a first convergence end and a second convergence end. The first convergence end is connected to the inlet flow path. The second convergence end is connected to the outlet flow path, and the two convergent ends have circular edges. (c) Adjusting the area through which the fluid flows by changing the flow rate of the fluid. 9 1271219 (d) β is further placed at one or more outlet channels at the end of the chamber and directs fluid flow to - or several out of the alpha flow path to collect fluid. According to the invention, the converging point of the convergent end of the cavity is a rounded edge' such that the cavity is wide at the center of the towel and narrower toward the portion of the population flow path and the end of the outlet flow path. Fluid flows through the inlet channel into the cavity' and is directed to one or more outlets for collection and analysis of the fluid. The above and other objects and advantages of the present invention will be described in detail below with reference to the accompanying drawings. [Embodiment] The first figure is a schematic view of a fluid guiding module according to a preferred embodiment of the present invention. Referring to the second figure, the fluid guiding module 200 includes two inlet channels 201a and 201b, two outlet channels 203a and 203b, and a cavity 202. The center of the cavity 202 has a gradually wide section. The cavity 202 is provided with a first convergence end 204a and a second convergence end 204b. In addition, the cavity 202 has a first stop 2021 and a second stop 2022. The cavity 202 begins to converge and the middle points toward the convergence end are defined as convergence points. The first converging end 204a is connected to the inlet flow paths 201a and 201b, and the second convergent end 204b is connected to the opening flow paths 203a and 203b. In the second figure, only two inlet flow paths and 10 1271219 two outlet flow paths are shown, and the number of inlet flow paths and outlet flow paths can be varied according to the needs of the actual application. As shown in the second figure, the front end of the first stopper 2021 has an acute angle, and the stopper 2021 is disposed at a convergence point of the acute angle toward the connection point of the first convergence end and the inlet flow path. The front end of the second stopper 2022 has a convex surface and faces the first stopper 2021' and this stopper 2022 is disposed at a convergence point of the laminar flow toward the outlet flow passage. The first stop and the second stop are such that when the fluid in the cavity is in a laminar flow state, the flow lines of the fluid within the cavity are parallel and nearly straight to each other. The dotted line in the second figure is the flow pattern of the fluid. By strategically arranging the stop 2021 and the stop 2022, fluid from the inlet flow paths 2a and 201b is guided to flow through the cavity 202 in a laminar flow. It is worth noting that the dashed lines are parallel where the central portion of the cavity 202 is gradually widened. By means of the central widening section of the cavity and the second stop, when the fluid enters the cavity, the separation of the fluid can be avoided, the distance required for the fluid to be fully developed can be shortened, and the flow line can be prevented from being twisted, so as to reduce the flow line becoming a straight line. Conversion area. When fluid enters the chamber, the flow rate at the outlet runner is not affected by the inlet channel fluid parameters because the fluid is fully developed. This specially designed chamber collects the same type of fluid at each outlet, which can be achieved by arranging appropriate outlet channel passages at appropriate η 1271219 δ spacing. According to the present invention, the cavity 202 can be a symmetric cavity whose flow lines are substantially parallel and nearly straight when the fluid in the cavity is in a laminar flow state. The shape of the front of the block 2021 in the shape of an asymmetric symmetric triangle may be more advantageous for guiding the fluid. The guiding principle of the fluid guiding module of the present invention is as follows. The flow directing module uses two or more inlet flow channels to direct fluid injected into the chamber, the cavity having a centrally widened section. Through the cavity of this special structure, when the fluid in the cavity is in a laminar flow state, the flow lines of the fluid are almost parallel to each other, and the fluids do not mix with each other. When the fluid from the inlet flow channel is injected into the cavity at the same flow rate, it will flow through the area of equal volume. On the other hand, when the fluid from the inlet flow channel is injected into the chamber at different flow rates, the volume of the area through which it flows will vary with the ratio of the inlet flow rate. By providing one or more outlet channels at the end of the chamber, the same type of fluid can be directed into one or more outlets to collect the fluid. In summary, the guiding process of the fluid guiding module can be summarized as follows: (a) First, two or more inlet flow channels are used to guide one or several fluids injected into the cavity. (b) Next, a cavity is used which has a first stop, a 12 1271219 second stop and a central progressive section. The front end of the first block has an acute angle. The end of the first block has a convex surface. Accordingly, when the fluid in the chamber is in a laminar flow state, the flow lines of the fluid are almost parallel to each other. The cavity has a first convergence end and a second convergence end. The first convergence end is connected to the inlet flow channel, and the first convergence end is connected to the outlet flow channel, and the two convergence ends have a circular edge. (C) Then, the area through which the fluid flows is adjusted by changing the inlet flow rate of the fluid. (d) Finally, one or more outlet flow passages are provided at the end of the chamber # and the pilot fluid is directed toward one or several outlet flow passages to collect the fluid. In addition, step (b) may further comprise a step of arranging the first stop at a convergence point close to the first convergence end and an acute angle thereof toward a connection point of the first convergence end and the inlet flow path. Similarly, step (d) may further comprise a step of arranging the second stop at a convergence point close to the second convergence end and having a convex surface facing the first stop. The function of the fluid guiding module of the present invention is related to the specific gravity of the fluid, the viscosity, the inlet flow rate, the diameter of the microchannel, the width of the converging section, and the shape and size of the cavity. Therefore, the above 13 1271219 factor can be used as the control parameter of the fluid guiding module. The second through third e-graphs illustrate the formation of different fluid flow patterns when the inlet flow rate into the inlet flow path changes. The third to third e diagrams take two inlet flow paths as an example, one of which is a top inlet flow path and the other is a bottom inlet flow path. Vl and V2 in the figure represent the inlet flow rates of the top inlet flow path and the bottom inlet flow path, respectively. In the third to third e-pictures, the ratio between ¥1 and ¥2 is 1:1, 1:2, 1:3, 1:4, and 1:5, respectively. As shown in the third to third e diagrams, when the inlet flow velocity of the bottom inlet flow passage is higher than the inlet flow velocity of the top inlet flow passage, the broken line is bent upward, and the fluid from the bottom inlet flow passage flows through the larger region. It is worth noting that the fluid will also flow to different outlet channels in proportion. As shown, the fluid injected from the bottom inlet flow path is directed toward the entire bottom outlet flow path and a portion of the top outlet flow path. Thus, the present invention directs fluid flow through different regions of the chamber and toward different outlet flow paths by adjusting the relative inlet flow rate of the fluid in the inlet flow path for collection and analysis of the fluid. As previously stated, the lower the flow rate of the inlet fluid, the smaller the volume of the area through which it flows. One of the advantages of the present invention as previously described is to reduce the distance that the fluid needs to be fully developed. Therefore, the volume of the region through which the different fluid flows and the cross sectional Width of the cavity, 14, 12912, maintain a special relationship with the flow velocity of the different fluid. The fourth figure shows a closure of the present invention. This example comprises two inlet flow channels 401a and 401b, a cavity 202 and five outlet flow channels 4〇2a to 402e, wherein the cavity 202 contains a triangular shape. The stopper 2〇21 and a circular stopper 2022. As shown in the third to third e-graphs, the fluid in the fourth figure can be directed to different outlet channels by varying the inlet flow rate of the fluid. In this design, one fluid can be directed to multiple outlet channels, or different fluids can be directed to the same outlet channel. For different applications, the number of inlet and outlet channels can vary, and the relative inlet flow rate can be varied to perform diverse detections and analyses, such as the presence of materials and the concentration of materials. • The fluid guiding module of the present invention provides the following advantages over conventional techniques. (1) When the fluid is in a laminar flow state, its flow lines are almost parallel. (2) By adjusting the ratio of flow rates between different fluids, a particular fluid can be directed to one or more specific outlet flow paths. (3) By adjusting the flow rate of the fluid, the time during which the fluid flows through the module can be dynamically adjusted. (4) The fluid guiding module has a wide range of flow rates and a simple structure, which makes it inexpensive to produce in a wide range of applications, such as cell culture, cell-to-drug detection or biochemical detection. 15 1271219 However, the above is only the preferred embodiment of the present invention, and the scope of the present invention is not limited thereto. That is, the equivalent changes and modifications made by the patent application scope of the present invention should still be within the scope of the present invention. , [271219 [Simple description of the diagram] The first figure shows a conventional differential absorption device for differential absorption with a structure of a 微-shaped microfluidic tube. The second figure is a schematic view of a fluid guiding group according to a preferred embodiment of the present invention. The third through third e-graphs illustrate the formation of different fluid flow patterns as the inlet flow fans entering the inlet flow path change. The fourth figure is an application example of the second drawing of the present invention. [Main component symbol description] 200 fluid guiding module 201a, 201b inlet flow channel 203a, 203b outlet flow channel 202 cavity 204a first convergence end 204b second convergence end 2021 first block φ 2022 second block vl, V2 inlet flow rate 401a inlet flow path 401b inlet flow path 402a, 402b, 402c, 402d, 402e outlet flow path 17