CN215506830U - Micro-fluidic chip based on Tesla valve - Google Patents
Micro-fluidic chip based on Tesla valve Download PDFInfo
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- CN215506830U CN215506830U CN202122019548.9U CN202122019548U CN215506830U CN 215506830 U CN215506830 U CN 215506830U CN 202122019548 U CN202122019548 U CN 202122019548U CN 215506830 U CN215506830 U CN 215506830U
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Abstract
The application relates to a micro-fluidic chip based on a Tesla valve, which comprises a sample injection chamber with a sample injection hole and a sample outlet hole, a plurality of mutually independent reaction chambers and a micro-fluidic flow channel, wherein the micro-fluidic flow channel comprises a sample injection main flow channel and a sample injection sub-flow channel, one end of the sample injection main flow channel is communicated with the sample outlet hole, the other end of the sample injection main flow channel is provided with sample sub-flow outlets the number of which is consistent with that of the reaction chambers, and each sample sub-flow outlet is communicated with the sample injection port of the corresponding reaction chamber one by one through one sample injection sub-flow channel; a Tesla valve is arranged on each sample injection branch channel, so that liquid in the sample injection branch channels enters the reaction chamber, the liquid in the reaction chamber cannot enter the sample injection branch channels, one-way circulation is realized, mutual pollution among the liquid in the sample injection process is avoided, and the accuracy and the efficiency of detection are improved. And the service life of the Tesla valve is long, the movement of a movable part is not needed to limit the circulation or the obstruction of liquid, and the service life of the microfluidic chip is prolonged.
Description
Technical Field
The utility model relates to the technical field of microfluidics, in particular to a microfluidics chip based on a Tesla valve.
Background
Microfluidic chips or Lab-on-a-chips refer to a technology for integrating basic operation units such as sample preparation, biological and chemical reaction, separation, detection, and the like, which are related in the fields of biology, chemistry, and the like, or basically on a Chip with a few square centimeters (even smaller) to complete different biological or chemical reaction processes and analyze products thereof. This technique is in principle suitable for the reaction, separation and detection of various types of molecules ranging from nucleic acids, proteins up to small organic and inorganic molecules. The micro-fluidic chip has the characteristics of controllable liquid flow, extremely less consumption of samples and reagents, ten-fold or hundred-fold improvement of analysis speed and the like, can simultaneously analyze hundreds of samples in a few minutes or even shorter time, and can realize the whole processes of pretreatment and analysis of the samples on line.
Microfluidic chips have been developed into a new research field crossing biological, chemical, medical, fluidic, electronic, material, mechanical, and other disciplines. The microfluidic chip droplet technology can accurately control generation and control of droplets, and uniform monodisperse droplets are generated, so that the microfluidic chip droplet technology becomes a high-throughput platform for biomedical and chemical research.
The common micro-fluidic chip can cause mutual pollution between liquids in the sample introduction process, and the detection accuracy and efficiency are reduced. Currently, valves are added to limit the flow of fluids to each other, but conventional valves require movable parts to limit the flow or blockage of fluids, which are easily damaged and have a short service life.
SUMMERY OF THE UTILITY MODEL
The utility model aims to provide a micro-fluidic chip based on a Tesla valve, which has high detection precision and long service life.
In order to achieve the purpose, the utility model provides the following technical scheme: a tesla valve-based microfluidic chip comprising:
a sample introduction chamber having a sample introduction hole and a sample exit hole;
the reaction chambers are mutually independent and are provided with sample inlets;
the microfluidic flow channel is arranged between the sample feeding chamber and the reaction chambers and is used for communicating the sample feeding chamber with the reaction chambers;
the microfluidic flow channel comprises a main sample injection flow channel and sub sample injection flow channels, and the number of the sub sample injection flow channels is consistent with that of the reaction chambers;
one end of the main sample injection channel is communicated with the sample outlet holes, the other end of the main sample injection channel is provided with sample sub-outlet ports with the number consistent with that of the reaction chambers, and each sample sub-outlet port is communicated with the sample inlet of the corresponding reaction chamber one by one through a sample sub-channel;
each sample feeding branch channel is provided with a Tesla valve so that liquid of the sample feeding branch channel enters the reaction chamber.
Furthermore, one part of the reaction chambers is positioned at one side of the main sample injection channel, and the other part of the reaction chambers is positioned at the other side of the main sample injection channel.
Furthermore, the reaction chambers are positioned on one side of the sample injection main flow channel.
Further, the sample introduction chamber comprises a sample introduction groove, and the sample introduction hole and the sample outlet hole are communicated through the sample introduction groove.
Further, the reaction chamber comprises a reaction tank, and the sample inlet is communicated with the reaction tank.
Further, the micro-fluidic chip based on the Tesla valve is also provided with an air exhaust device, and the air exhaust device is used for adjusting the pressure in the sample feeding chamber and the reaction chamber.
Further, the gas exhaust device comprises an exhaust main runner, an exhaust branch runner and an overflow channel which are connected with the sample injection chamber and the reaction chambers, and the number of the exhaust branch runners and the number of the overflow channels are consistent with that of the reaction chambers;
the venthole of advance kind cavity with the one end intercommunication of exhaust sprue, the other end of exhaust sprue be provided with figure with the exhaust convergent port that reaction chamber figure is unanimous, each exhaust convergent port of exhaust sprue all loops through exhaust subchannel, overflow channel and corresponding reaction chamber's inlet port intercommunication one by one.
Further, each exhaust gas branch passage is provided with a Tesla valve so that gas of the exhaust gas branch passage enters the reaction chamber.
The utility model has the beneficial effects that: the application provides be provided with the tesla valve on every kind subchannel to make the liquid that advances kind subchannel get into reaction chamber, and reaction chamber's liquid can't enter into kind subchannel, realizes the one-way circulation, avoids can causing the mutual pollution between the liquid at the kind in-process, improves the accuracy and the efficiency that detect. And the service life of the Tesla valve is long, the movement of a movable part is not needed to limit the circulation or the obstruction of liquid, and the service life of the microfluidic chip is prolonged.
The foregoing description is only an overview of the technical solutions of the present invention, and in order to make the technical solutions of the present invention more clearly understood and to implement them in accordance with the contents of the description, the following detailed description is given with reference to the preferred embodiments of the present invention and the accompanying drawings.
Drawings
Fig. 1 is a schematic structural diagram of a tesla valve-based microfluidic chip according to an embodiment of the present application;
fig. 2 is another schematic structural diagram of a tesla valve-based microfluidic chip according to an embodiment of the present application;
fig. 3 is a schematic view of another structure of a tesla valve-based microfluidic chip according to an embodiment of the present application.
Detailed Description
The technical solutions of the present invention will be described clearly and completely with reference to the accompanying drawings, and it should be understood that the described embodiments are some, but not all embodiments of the present invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplicity of description, and do not indicate or imply that the mechanism or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.
In the description of the present invention, it should be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, e.g., as meaning either a fixed connection, a removable connection, or an integral connection; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art.
In addition, the technical features involved in the different embodiments of the present invention described below may be combined with each other as long as they do not conflict with each other.
Referring to fig. 1, a tesla valve-based microfluidic chip 100 according to an embodiment of the present application includes a sample chamber 1, a plurality of reaction chambers 2, and a microfluidic channel 3.
The sample chamber 1 has a sample inlet (not shown) and a sample outlet (not shown). The sample liquid enters the sample chamber 1 through the sample inlet hole.
The reaction chambers 2 are independent of each other, and the reaction chambers 2 have injection ports (not shown). The specific number of the reaction chambers 2 can be set according to actual needs, and is not particularly limited.
The microfluidic flow channel 3 is arranged between the sample injection chamber 1 and the reaction chambers 2 and is used for communicating the sample injection chamber 1 with the reaction chambers 2. The microfluidic flow channel 3 comprises a main sample injection channel 31 and sub sample injection channels 32, and the number of the sub sample injection channels 32 is the same as that of the reaction chambers 2.
One end of the main sample injection channel 31 is communicated with the sample outlet, the other end of the main sample injection channel 31 is provided with sample branch outlet ports (not shown) with the number consistent with that of the reaction chambers 2, and each sample branch outlet port is communicated with the sample inlet port of the corresponding reaction chamber 2 one by one through one sample branch channel 32. That is, the reaction chamber 2 is communicated with the main injection channel 31 through one sub-injection channel 32, so that the communication between the injection chamber 1 and each reaction chamber 2 is realized. The liquid flows to the main injection runner 31 through the sample outlet of the injection chamber 1, then enters each sub-injection runner 32 from the sub-sample outlet, and then flows into the corresponding reaction chamber 2.
The main sampling channel 31 and the sub sampling channels 32 may be linear structures or curvilinear structures, and are not limited herein.
Some in a plurality of reaction chamber 2 are located one side of kind main flow channel 31, and another some in a plurality of reaction chamber 2 are located the opposite side of kind main flow channel 31 to make rationally distributed.
Referring to fig. 2, a plurality of reaction chambers 2 may also be disposed at one side of the main injection channel 31.
In other embodiments, the distances between the reaction chambers 2 and the main injection channel 31 may also be different, so that the reaction chambers 2 are arranged in a staggered manner, and the specific arrangement of the reaction chambers 2 is not specifically limited herein.
Each sample inlet branch channel 32 is provided with a tesla valve 4, so that the liquid in the sample inlet branch channel 32 enters the reaction chamber 2, and the liquid in the reaction chamber 2 cannot enter the sample inlet branch channel 32.
The tesla valve 4 is a passive one-way conduction valve of fixed geometry that allows one-way fluid flow. The valve has a fixed geometric shape, so that the defect that the traditional valve is easy to damage due to the requirement of a movable part is overcome, and the valve can replace a movable valve. Because the fluid has inertia, when the fluid passes through the valve in different directions, the flow resistance is different, thereby realizing one-way circulation, avoiding the mutual pollution between the liquids in the sample introduction process and improving the accuracy and the efficiency of detection. And the service life of the Tesla valve 4 is long, the movement of a movable part is not needed to limit the circulation or the obstruction of liquid, and the service life of the microfluidic chip 100 is prolonged. The specific structure of the envoy valve is the prior art and will not be described herein.
The sample chamber 1 further comprises a sample groove (not shown), and the sample inlet and the sample outlet are communicated through the sample groove. The reaction chamber 2 comprises a reaction tank, and the sample inlet is communicated with the reaction tank.
The tesla valve 4 based microfluidic chip 100 is further provided with a gas venting means (not shown) for adjusting the pressure in the sample chamber 1 and the reaction chamber 2.
Specifically, the gas exhaust device comprises an exhaust main runner, an exhaust branch runner and an overflow channel which are connected with the sample injection chamber 1 and the reaction chambers 2, and the number of the exhaust branch runners and the number of the overflow channels are consistent with that of the reaction chambers 2.
The sample introduction chamber 1 is provided with an air inlet hole and an air outlet hole, the reaction chamber 2 is also provided with an air inlet hole and an air outlet hole,
the air outlet of the sample injection cavity 1 is communicated with one end of the main exhaust channel, the other end of the main exhaust channel is provided with exhaust gathering ports with the number consistent with that of the reaction cavities 2, and the exhaust gathering ports of the main exhaust channel are communicated with the air inlet of the corresponding reaction cavity 2 one by one sequentially through the exhaust branch channels and the overflow channels.
Every exhaust subchannel has tesla valve 4 to make the gas of exhaust subchannel get into reaction chamber 2, and reaction chamber 2's gas can't get into the exhaust subchannel in, avoid reaction chamber 2's liquid to follow gas and enter into the exhaust subchannel in, thereby improve the accuracy that detects.
Referring to fig. 3, a plurality of sample chambers 1 and reaction chambers 2 communicated with the sample chambers through microfluidic channels 3 may also be disposed on the microfluidic chip 100 based on a tesla valve, so as to improve the space utilization of the microfluidic chip 100.
In conclusion, what this application provided is provided with the tesla valve on every kind subchannel to make the liquid that advances kind subchannel get into reaction chamber, and reaction chamber's liquid can't enter into kind subchannel, realize one-way circulation, avoid can causing the mutual pollution between the liquid at the kind in-process of advancing, improve the accuracy and the efficiency that detect. And the service life of the Tesla valve is long, the movement of a movable part is not needed to limit the circulation or the obstruction of liquid, and the service life of the microfluidic chip is prolonged.
The technical features of the embodiments described above may be arbitrarily combined, and for the sake of brevity, all possible combinations of the technical features in the embodiments described above are not described, but should be considered as being within the scope of the present specification as long as there is no contradiction between the combinations of the technical features.
The above-mentioned embodiments only express several embodiments of the present invention, and the description thereof is more specific and detailed, but not construed as limiting the scope of the utility model. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the inventive concept, which falls within the scope of the present invention. Therefore, the protection scope of the present patent shall be subject to the appended claims.
Claims (8)
1. A tesla valve based microfluidic chip comprising:
a sample introduction chamber having a sample introduction hole and a sample exit hole;
the reaction chambers are mutually independent and are provided with sample inlets;
the microfluidic flow channel is arranged between the sample feeding chamber and the reaction chambers and is used for communicating the sample feeding chamber with the reaction chambers;
the microfluidic flow channel comprises a main sample injection flow channel and sub sample injection flow channels, and the number of the sub sample injection flow channels is consistent with that of the reaction chambers;
one end of the main sample injection channel is communicated with the sample outlet holes, the other end of the main sample injection channel is provided with sample sub-outlet ports with the number consistent with that of the reaction chambers, and each sample sub-outlet port is communicated with the sample inlet of the corresponding reaction chamber one by one through a sample sub-channel;
each sample feeding branch channel is provided with a Tesla valve so that liquid of the sample feeding branch channel enters the reaction chamber.
2. The tesla valve-based microfluidic chip of claim 1, wherein a portion of the number of reaction chambers is located at one side of the sample main channel and another portion of the number of reaction chambers is located at another side of the sample main channel.
3. The tesla valve-based microfluidic chip according to claim 1, wherein the plurality of reaction chambers are located at one side of the sample main channel.
4. The tesla valve-based microfluidic chip of claim 1, wherein the sample chamber comprises a sample well, and the sample inlet and outlet are in communication through the sample well.
5. The tesla valve-based microfluidic chip of claim 1, wherein the reaction chamber comprises a reaction channel, and the sample inlet is in communication with the reaction channel.
6. The tesla valve-based microfluidic chip according to claim 1, wherein the tesla valve-based microfluidic chip is further provided with a gas venting device for adjusting the pressure in the sample chamber and the reaction chamber.
7. The tesla valve-based microfluidic chip according to claim 6, wherein the gas exhaust device comprises an exhaust main runner, an exhaust branch runner and an overflow channel connecting the sample injection chamber and a plurality of the reaction chambers, and the number of the exhaust branch runner and the overflow channel is the same as the number of the reaction chambers;
the venthole of advance kind cavity with the one end intercommunication of exhaust sprue, the other end of exhaust sprue be provided with figure with the exhaust convergent port that reaction chamber figure is unanimous, each exhaust convergent port of exhaust sprue all loops through exhaust subchannel, overflow channel and corresponding reaction chamber's inlet port intercommunication one by one.
8. The tesla valve-based microfluidic chip of claim 7, wherein each of the exhaust gas sub-channels has a tesla valve thereon to allow gas of the exhaust gas sub-channel to enter the reaction chamber.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202122019548.9U CN215506830U (en) | 2021-08-25 | 2021-08-25 | Micro-fluidic chip based on Tesla valve |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202122019548.9U CN215506830U (en) | 2021-08-25 | 2021-08-25 | Micro-fluidic chip based on Tesla valve |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2024041209A1 (en) * | 2022-08-22 | 2024-02-29 | 深圳市卓润生物科技有限公司 | Microfluidic chip |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2024041209A1 (en) * | 2022-08-22 | 2024-02-29 | 深圳市卓润生物科技有限公司 | Microfluidic chip |
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