TWI226437B - Image display type biochip instrument - Google Patents

Abstract

The invented image display type biochip instrument at least contains the followings: a light source for emitting light beam; a collimating lens for making light beam emitted from light source form large-area parallel light that passes through the biochip to excite the fluorescent material on the biochip and to emit fluorescent light; a focus lens for focusing fluorescent light; a filter plate for removing the interference light wave; and a CCD camera for extracting image of the fluorescent light signal. The image extracted by CCD camera can be converted by an image converting apparatus into the data so as to become convenient for determining the fluorescent light intensity of each analyzed sample point. The image display type biochip instrument uses the collimating lens to make laser light source generate large-area laser-light excitation light source such that each sample point can receive high efficiency excitation light source. In addition, by using CCD camera, large-area fluorescent light image of biochip can be extracted quickly to perform the analysis such that the analysis time is dramatically reduced.

Description

1226437 V. Description of the invention (1) The technical field to which the invention belongs The present invention relates to a bio-chip reading device, and particularly 'ΤΛ r _____ η ____ 1 ^ J τχ _ _ · ~ ^' Jin] is a CCD (Charge-Coupled Device, Charge consumable strains—devices that analyze images after they are used to extract images. Couplings ... cattle) There are many analysis tools or analysis methods available for cameras to capture uranium in the prior art. Proteins and other biomolecules are available, but these analysis methods are It is often necessary to take a considerable time to know the analysis of 2 samples. When analyzing a large number of samples, it is often caused by too many samples. In addition, it is completed in China, so it is easy to cause errors in the analysis data. For the analysis of proteomics in one analysis, it is often necessary to simultaneously analyze the factors and analysis tools. Therefore, the need for more efficient biochips has the advantage of being able to analyze a large number of samples at the same time. This kind of analysis tool can save the manpower and material resources for analysis, so it has potential for application in f-text, for example, to find a stand-up, with the performance mechanism of Xi Fang '"drug screening and research genes, etc." Taking DNA as η 幺, ☆, as shown in the first picture, first. Day, day, 々 丨 L 'its detection method is large) The surface is placed as a solid load (such as slope A piece of silicon or a silicon crystal is formed into a DNA chip (0, which is generally also called a DNA microarray by grasping the needle / known DNA fragment (2). Two other needles (2 ^ 疋 are arranged in an array, on the other hand , Unknown DNA fragment under test

$ 6 pages 1226437 5. Description of the invention (2) (3) (Target MA, target DNA) is labeled with a fluorescent label molecule. Next, the DNA wafer (1) is hybridized with the target DNA (3). After the washing step, the DNA wafer (1) is left with only the MA fragment bound to the MA probe, and then passed through the biochip instrument. Scan 'to measure the fluorescence emitted by the fluorescently labeled molecules, and you can get the analysis result. In order to obtain the analysis results more quickly and accurately, the biochip reader that reads the analysis results of biochips has attracted considerable investment from researchers. At present, the most common biochip instrument uses laser light as the excitation light source and photomultiplier tube as the light detector. Its component structure is roughly shown in the second figure. This biochip (4) is a light beam emitted by a laser source (40). This laser beam passes through a focusing lens (41), is reflected on a beam splitter (42), and then passes through another focus. The lens (43) reaches the surface of the wafer to be measured (44). The fluorescently labeled molecules on the wafer (44) are excited by the laser to emit fluorescence (45) by the base, and the fluorescence (45) passes through the focus (43) , Beam splitter (42), focusing lens (46), and then filter (47) to filter out the light waves of the excitation light source, ^ electric multiplier tube (-ltipller tube (PMT) (JU to (48) to convert the optical signal It is an electronic signal and the signal analyzer needs to analyze all the samples on the biochip, and the optical signals of the positive array are very time-consuming. U is equipped with an excitation light source, and uses high-resolution white light with a wide pixel ^ c '此种 as this kind of biochip. Although the image is not captured by a Thai machine for analysis. The sample points are scanned one by one, but ^

1226437 V. Description of the invention (3) The excitation efficiency of the white light source is poor, so its analytical sensitivity is not as good as the aforementioned type of biochip instrument. If you want to increase the sensitivity of the analysis, it still takes a considerable time to get a higher fluorescence signal value. In summary, if we can develop a biochip analyzer that can quickly read the results of biochip analysis, the utility of the biochip analysis system will be greatly improved. SUMMARY OF THE INVENTION One of the objects of the present invention is to provide an imaging biochip, which uses a large area of laser light to simultaneously excite a sample on a biochip, and uses a large lens CCD camera to capture the signal as an image for analysis. , So you can achieve the purpose of rapid detection. The imaging biochip of the present invention includes at least: a light source for emitting a light beam; and a collimating lens (Collimating Lens) for forming a large area of parallel light emitted by the light source, the parallel light Light passing through the biochip can cause fluorescent substances on the biochip to be excited into fluorescence; a focusing lens for focusing the fluorescence; a filter; it is used to filter out interference light waves; and a CCD Camera for capturing fluorescent signals as images. The image captured by the CCD camera can be converted into data through an image data conversion device to facilitate the interpretation of the fluorescence intensity of each analysis sample point. -Because the imaging biochip of the present invention uses a collimating lens to make the laser light source generate a large area of laser light excitation light source, each sample point can receive a high-efficiency excitation light source at the same time. In addition, the development type of the present invention

1226437 V. Description of the invention (4) The object wafer instrument can use a CCD camera to quickly and quickly capture fluorescent images of biological wafers for analysis, thus greatly reducing analysis time. The embodiments of the present invention will be further described below with reference to the drawings. The following examples are used to clarify the present invention and are not intended to limit the scope of the present invention. Any person skilled in the art will not depart from the present invention. Within the spirit and scope of 'can be changed and retouched slightly, so the scope of protection of this creation shall be determined by the scope of the attached patent application. Stomach _ The third figure is an embodiment of the imaging biochip (5) of the present invention. The biochip array analyzer (5) shown in the second figure includes a light source (50) for emitting a light beam; a collimating lens (51) for forming a large area of the light beam emitted by the light source Parallel light, which passes through the biochip (52), so that the fluorescent substance on the biochip (52) is excited to emit fluorescent light; a focusing lens (53) is used to focus the fluorescent light; a filter Film (54); it is used to get rid of interference light waves; a large lens CCD camera (55), which is used to capture the fluorescence ~ signal as an image; a bearing platform (57), which is used to It carries a biochip (52); and an image data conversion device (56), which can convert the image signal into data to facilitate the interpretation of the fluorescence intensity of each analysis sample point. When analyzing the biochip by using the imaging biochip of the present invention, the biochip is first placed on a carrying platform (57). During the analysis, a laser light source (50) emits a light beam, and the light beam passes through a collimating lens (5 丨) to form a large area of parallel light, which passes through the biochip (5 2), so that the biochip

1226437 V. Description of the invention (5) (52) The fluorescent substance is excited to emit fluorescence. After the optical signal is focused (53) and the interference light wave is filtered by a filter (54), the CCD phase processes the fluorescent signal as an image, and this image signal is transmitted to the image number) device (56), and the The image signal is converted into data. In order to make the analysis of the data more convenient, the image data conversion device (56) can be a computer, and the two (56) can also include a Dian type that controls the movement of the biochip carrying platform (57), so that the imaging type biochip The instrument (5) is easier to operate. king

In summary of the above descriptions and embodiments, the imaging type 2 collimating lens of the present invention enables the laser light source to generate a large area of laser light excitation light source, so that each sample point simultaneously receives a high-efficiency excitation light source. In addition, the use of a CCD camera to quickly and accurately capture the fluorescence image of a biochip and then analyze it can greatly reduce the analysis time.

1226437 Brief description of the diagram The first diagram is a schematic diagram of the conventional DNA wafer measurement. The second figure is a schematic diagram of a conventional biochip instrument. The third figure is a schematic diagram of an imaging biochip of the present invention. Description of component symbols 1 DNA wafer 1 4 Conventional biological wafer 4 1 Focusing lens 42 44 wafer 45 47 filter 48 5 imaging 51 collimator lens 52 54 filter 55 57 bearing platform DNA probe 3 instrument of the present invention 40 Beamsplitter 43 Fluorescence 46 Photomultiplier tube 49 Biochip 50 Biochip 53 CCD camera 56 Target DNA Laser light source focusing lens focusing lens Computer light source focusing lens image data conversion device

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Claims (1)

1226437 VI. Application Patent Scope 1. An imaging biochip, which includes at least: a light source for emitting a light beam; a collimating lens for forming a large area of parallel light from the light beam emitted by the light source, The parallel light passes through the biochip, so that the fluorescent substance on the biochip is excited to emit fluorescence; a focusing lens for focusing the fluorescence; a filter; it is used for filtering out interference light waves; and A CCD camera is used to capture the fluorescent signal as an image. 2. The imaging biochip apparatus described in item 1 of the scope of patent application, the imaging biochip apparatus further includes an image data conversion device, which can convert the image signal captured by the CCD camera into data. 3. The imaging biochip apparatus described in item 2 of the scope of patent application, the imaging biochip apparatus further comprises a carrying platform for carrying the biochip, and the carrying platform can move in the X-axis direction. 4. The imaging biochip apparatus described in item 3 of the scope of patent application, the imaging biochip apparatus further includes a computer, the computer including at least a set of control parameters that control the moving direction of the bearing platform. 5. The imaging biochip device as described in the scope of patent application: Item 4, wherein the computer can be used as an image data conversion device, which includes at least one group that can convert the image signals captured by the CCD camera into data Parameters.