TW490286B - Method of manufacturing liquid inoculum of mushroom and inoculating device for the same - Google Patents

Abstract

Pre-incubation process of main liquid start culture used to adjust main culture from a start culture inoculated from the stock culture with liquid inoculation method and the main incubation process for the above liquid start culture to put into a fixed-volume culture tank 30 are characterized in the production method including the above two incubation processes for liquid inoculum of mushroom after the inoculating broth is housed in the culture tank 30; the culture tank 30 holding the inoculating broth is put into an autoclave to sterilize and then allowed to cool to room temperature and the main start culture of the liquid inoculum prepared by the pre-incubation process disclosed above is cultured in the culture tank 30.

Description

V. Description of the invention V. Description of the invention Fifth, the cultivating fungus is equal to the method of post-cultivation of the cultivating cultivar '< Scope of the invention > of the ϋΓί: the method for producing liquid seed bacteria in Shanz and the liquid species < background of the invention > f. Mushrooms such as Pleurotus edodes and beech wood are cultivated withered, inoculated, cultivated and cultivated. These cultivation methods of shiitake mushrooms, of course, are different depending on the shiitake mushroom breeding * ',,, and post-harvest environment and the cultivation process. It is determined that they are planted according to these cultivation methods. Cultivation method industry cultivation, the commonly used species ",; belongs to. ',,,,, and species of S. spp. Made from cultivated land and the use of solid species such as cereals :: these sawdust species and fungi are filled with sawdust to support Γ: Inoculation, culture and preservation: strain =, which is the same as that used to grow the inoculum in the lane 4 and ° breeding bottles. The same procedure for making this kind of inoculum is used to cultivate the inoculum, and one is inoculated in the inoculum. … The cultivation method: Γ As above, the use of shiitake mushrooms such as Saw spp. Is used to grow trees. ”In terms of the process of making sawdust and fungi, it takes about 25 to 3 G days to ensure that one species is cultivated. The space for cultivating flasks is in the center where the inoculum is supplied. 5. Description of the invention (2) _—_ Uses a very large space. In addition, Miao Du should prepare multiple culture flasks. Culture flasks: The second culture flask should be used to cultivate inoculum. It is necessary to clean the facilities to organize the cultivation environment. It is also necessary to set the position of the culture bottle in the training facility: there is a problem such as the alignment. ', There is no difference in breeding and fruiting. When sawdust seed fungi are used, there is no ^ goodness of cultivating shiitake mushrooms when inoculated with seed fungi. If there is a problem, the inoculation of Saw genus bacteria is equivalent to the field ancient machine inoculation. There is a problem that the inoculation time is too long. The person must always guard the inoculum inoculating machine. In order to solve these problems, the present invention aims to provide a method for producing inoculum and a liquid "inoculation of bacteria" = liquid liquid inoculum instead of sawdust inoculum, etc ... .., It is used for the artificial cultivation of Lentinula edodes and can be cultivated in two ways, so that the inoculation of sub-bacteria can be easily applied, and the production ... upper: In order to achieve the purpose of the disclosure, the present invention has a structure of unveiling. In the method for producing a liquid seed fungus of the scent of the present invention, the method includes the step of cultivating the liquid seed fungus obtained by cultivating the seed fungus obtained by cultivating the liquid fungus with a liquid culture method; and cultivating the liquid seed fungus before the culture; and The inoculum of the preparation liquid is put into the culture process in which the inoculum is stored and used for inoculation-quantitative culture liquid, and is cultured in § 19 culture liquid. After accommodating the culture solution in the culture tank, the entire culture tank is stored in a high-pressure sterilizer and pressured.

Page 5 490286 V. Description of the invention (3) Sterilization, the characteristics of the nutritional preparation prepared by the high-pressure process. The cultured bean powder and sugar are introduced into the air during the cultivation. Also, it is best to cultivate the tank in a gas-free manner. In addition, it is more suitable to open the body and use the switch. In the connection, the culture tank of the inoculation solution and bacteria is passed through the mouth, and the self-cultivation tank controlled by the system is provided for the empty of the air valve which is ejected by the nozzle. The volume conveying structure is structured, and the user is provided with a solution corresponding to the germicidal liquid inoculum. The cultivation project is good for cultivating the introduction of the tank body into the gas-filled closed valve and the air valve. 0 kinds of liquid seeds and bacteria are connected to the connecting pipe and nozzle to open the liquid body and the bacteria are used to control the predetermined number of bottles. Cool to normal temperature, then inoculate the culture medium in the culture tank, and then use the culture medium of the original culture medium, preferably in distilled water, and add medium and large temperature to the normal temperature tank. You can ϊδ, close your eyes and cry. Use an air duct to connect the air from the air filter to adjust the air pressure in the tank body. ^ The inoculation section for cultivating the mushroom seed liquid cultivation ground is provided with a communication channel. When the liquid field seedlings are closed, the control unit added to the cultivation tank for inoculating them is inoculated, and the air valve of the nozzle is used to fill the cultivated cultivation bottle at a predetermined opening and closing time of the air valve of the control unit. At this time of the liquid seed bacterial inoculation position, and the gas filter, the air pressure of a container at the bottom of the container, and the input portion to the tank culture tank is used at the same time. Feature. The central control sends the timepiece from the central control to order the container conveyor

Page 6 490286 The inoculation section of the mouth is described in detail for each row of cultivated bottle culture bottles > Detailed description of the method of the present invention, which is characterized by the cultivation of liquid inoculum that is used for cultivation . The method needs to be able to V. Description of the invention (4) The cultivation bottle is equipped with the spray. It is suitable to arrange the container in a convenient way to be accommodated in the container. < Preferred specific embodiments The cultivation process of the present invention and the inoculation liquid for seed culture and inoculation of liquid inoculum production process of the present invention are described in detail below with reference to the accompanying drawings.) During the cultivation of shiitake mushrooms, it is necessary to reliably provide the required inoculum, so the Party fungus. The embodiment of the quantitative conveying mechanism is a person who conveys in a pan, and arranges the position of the mouth. Contains the liquid inoculum making process. The following describes the process of liquid Peziz in order. Occasionally, a large number of artificial cultures that can efficiently produce fragrant guava are easy to reconcile: the germs for the consumption μ ΐ 1 ~ 3 are the adjustment processes of germs used for pre-cultivation of germs, seed, rigid, and rearing processes' and An explanatory diagram of this culture process of seed bacteria. Fig. 1 shows the preparation of seed bacteria used in the preculture.

The test tube containing the strain is stored in the test tube. From this test tube 10, the strain is inoculated on the plate, and the culture is performed. The cultivated material used for the flat cultivation 12 can be used as the cultivated material used in the bacterial culture commonly used in ‘PDA cultivation and fish gel culture. Step S1 is a process of cultivating and cultivating a strain on a plate. In this example, the cultivation temperature of the cultivating step of Pleurotus edodes seedlings is 2 (pc ~ 25 ^). The cultivation 14 indicates the state in which the strain is cultured on the flat cultivation ground 12. As the cultivation progresses, the bacteria grow. The silk is spreading in the cultivation ground. 6 indicates that the cultivation is completed on a flat plate. 2

Page 7 V. Description of the invention (5) _ Yang, cut off the state of Yu · ψΑ | In the pre-culture, it is necessary to make Fan 9 with the main mule 5 ~ 6 and 18 slices with small slices. Refers to 4 inside, two liquid two? Pre-cultivation process. The so-called pre-culture, system. In the case of the present culture species g, the preparation of the used bacterial strain was cultured. Anteriorly separated liquid seed bacteria are inoculated into the culture medium. < Preparation of liquid seed bacteria used in this culture > * 50. τηΓ is a container for culturing liquid seed bacteria. In the present example, a container 20 containing a culture fluid for bacteria was used in a 5 ml grain of diatrozole. Cultivated seeds: Soybean is added to the water to limit it. In this embodiment, a steaming room is used, which is a culture solution that uses ^ ν sugar as a nutrient. After adjusting the nutrient solution, each-Erlenmeyer flask container 2 is dispensed separately. . :. In the sterilization process of the culture solution injected into the container 20 by ΓΓϋΐ. Example The yoke force yoke was autoclaved at 121 C for 15-20 minutes. Step S3 is a process in which the culture solution is cooled to normal temperature after being sterilized. Step S4 is the improper inoculation of the pre-made inoculum to the σ nutrient solution by using the flat cultivation ground. The microtome 8 of the above-mentioned cultivated flat plate 1 12 was put into 4 to 5 containers 20. ^ Step S5 is a process of leaving the culture solution inoculated with the seed bacteria and culturing. Only the & example was left to stand for 4 days in a room adjusted to 20 ° C to 2 5 t: about room temperature. This period is the pre-culture period. Step S6 is a process of culturing by shaking culture after standing culture. The culture phase of f is similarly cultured at 20 t ~ 25 ° C for about 16 days. In this example, an oscillation culture machine was used, and the oscillation was performed at a rotation speed of 100 rpm. Oscillation

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The sterilization process uses a high-pressure sterilization machine used for high-pressure cultivation. The rotation number of the high-pressure sterilization machine can be appropriately adjusted between about 50 and 150 rpm. ^ When the culture is close to completion, mycelium is elongated in the culture solution. In the case of using this solution to culture the bacteria, it is best to use a homogenizer and the like to subdivide the mycelium before use. After subdividing, a faster hypha distribution can be obtained in the culture. The above pre-culture process can be completed in about 20 days. Once the cultivation of the inoculum was completed by culturing, it was entered into the present culture. Fig. 3 shows the present culture process. In the present culture, the culture tank 30 containing the culture solution is used, and the liquid seed bacteria cultured in the culture before inoculation are cultured in the culture solution. The thin structure of the culture tank 30 will be described later. Only the culture tank 30 uses a stainless steel plate to form a bottom-closeable container. Of course, materials other than stainless steel plates can also be used, and not only metal plates, but also plastic materials. The size of the culture tank 30 may be appropriate. In this embodiment, a culture tank of 60 cm in diameter and 1.2 m in south of the tank body 32 is used. The upper part of the tank body 32 is provided with an inoculation port 34 for injecting liquid seed bacteria, and a viewing window is provided on the side and the top of the tank body 32. In this culturing process, first, the culture f stored in the culture tank 30 is prepared and divided into seven points. Fully inject the culture solution into the culture tank 30. The culture medium has an appropriate composition. In this embodiment, soybean powder: sugar is added as the nutrient to distilled water. In the case of withering the culture solution without preparing the culture solution in advance, it is best to add the nutrient solution while stirring the nutrient solution to uniformly dissolve the nutrient solution. Step S7 is the sterilization process of the culture solution. This sterilization, in this embodiment, uses traditional mushrooms

490286

Dad, it ’s germinated. Example The sterilized dad used for cultivating the inoculum culture in shiitake mushrooms will be stored in step S8. The filter placed in the culture tank 30 here is the efficiency of pouring the cold water into the culture tank 30. If it is cooled to normal temperature and contained in a culture tank 30, it will be sterilized by filling it with sawdust during artificial cultivation at 121 ° C at 50 ° C. Such a liquid sterilization method belongs to the culture liquid in the culture tank 30. During the sterilization process, it is best to cool the culture liquid by killing the bacteria in order to prevent the invasion of the bacteria and accelerate the cooling. The benefits are more effective. Of course, when the volume is large, cold water can be poured and the cold water method should be used. Then the inoculum is inoculated to a next culture tank, 30 high-pressure sterilization, and 80 minutes of sterilization. It is equal to the use of high-pressure after cultivating bottles. High-pressure sterilization is a very effective method for sterilizing the liquid. The trough 30 is cooled to room temperature and the air is blown in immediately after the bacteria have passed. When the air is blown in, it should be in the air. Cold water is poured from the shoulder of the culture tank 30 with a device, and the cold water is only poured, but the accelerated cooling can improve the operation. Usually it can be done in half a day to a day.

Step S9 is a process of connecting the liquid seed bacteria prepared in the pre-cultivation process to the culture solution after cooling. When inoculating the liquid seed bacteria, you must pay full attention to prevent the incorporation of mixed bacteria. In this embodiment, the inoculation operation is performed in a clean room. "Before opening the inoculation port, the periphery of the inoculation port is subjected to flame sterilization." Secondly, a few cc of propanol is dripped into the flange portion of the inoculation port, and the inoculation is started by the ignition. Open 'Inject the liquid seed bacteria prepared in the pre-cultivation process in the culture tank 30 while the propanol flame is generating an air current. In the examples, 2 to 3 branches of liquid inoculum used in the Erlenmeyer flask used in the preculture process were introduced. Immediately after the injection of liquid seed bacteria was completed, the inoculation port was closed.

Page 10 490286 V. Description of the invention (8) '~' ---- ~ One Step S10 is the cultivation process. Transfer to the liquid culture tank 30 and start sonication in the culture room. + Jiaoli 70 Xin silly 旳 you are dangerous "τ Nao Shi; t The room temperature of the meal culture room is maintained at 22 ~ 23 t privacy. One side enters the air in the culture tank 30, and the other is passed through an air filter as the medium, 俾This will prevent the miscellaneous people from invading the culture tank. = The time required to cultivate in U0. It takes 7 ~ 8 days for pushuxiangzi to wait. His appointment takes about U to 12 days. Culture tank 3. Progression of internal culture: The state of the culture medium can be easily seen from the inspection window.捭 Medium: Mycelium will elongate and can be inoculated according to the culture status, good or bad. Another kind of nourishing fluid can confirm that the species is not known. "Because it is true that the odor during cultivation can also be easily used in practical cultivation :: No, it can prevent the use of ore debris and fungi such as bad seed coats. It's more traditional. 'Assumption of bad seed bacteria, < Tongue% liquid seed bacteria inoculation process, so the preparation of two or two liquids can be sterilized'. It is a short time to recover within 4 rooms, which is also more suitable. This picture is not suitable for liquids. The structure of the culture tank 30 of the inoculum culture does not record the steel plate to form a bottomed cylindrical body. As mentioned above, the storage tank is stored in the culture tank ^ Because it is in the high-voltage potential "Father '," 疋 1Ί ~ buttocks " Inside the sign with the culture tank 3 0 — Lifting the mouth to kill the tank body 32 with high resistance design and manufacture. 33 is installed in the tank = 槽 ^ ::: using casters. In the storage culture tank 30 under high pressure sterilization dad Ϊ Moving: When raising the tank 30, you can use the casters 33 to move it. The airtight opening and closing is installed on the upper part of the tank body 32 in an openable and closable manner.

Page II 490286 V. Description of Invention (9) Cover 38. This opening / closing lid 38 is opened and closed when a culture liquid is added to the culture tank 30 or the like. The diameter of the opening / closing lid 38 may be made larger by stirring while the culture medium is being added. 40 for the seed fungus. The inoculum input port 40 is mounted on the flange 40b of the switch cover 38, and the plug 40a is detachably installed. When the inoculum is introduced into the culture tank 30, the cock 40a is removed. An inflating tube 42 is introduced into the tank body 32. The tube 42 forms a ring around the bottom of the groove body 32, and opens most air holes. The officer 42 is connected to a piping outside the tank body 32, and is connected to an air input section 46 such as a compressor through an air filter 44 and an on-off valve 45. The air filter ❶ 4 4 π filters the air sent from the air input section 46 to prevent the invasion of germs and send it into the culture tank 30. In addition, the air sent from the air input section 46 is introduced through the air filter 44 and the on-off valve 48 through the manifold 49 connected to the upper part of the tank body 32 to the second row of the 50/50 series manifold divided by the manifold 49. Trachea, 52 is an on-off valve that controls the row rolling of 't' f0. Reference numeral 54 is for inspecting the pressure in the culture tank 30, and 36 is an inspection window provided on the side of the body 32. = 2 Please simmer the broth. Inspection window 3 = the position where the liquid level of the culture medium and the progress of the culture are stored. The visual edge r Ϊ: When the culture tank 30 of this example is used, the situation as described above is sterilization and product; when 7 P culture solution, air is continuously fed from the air input part to = 32: and from the exhaust The tube 50 discharges a large amount of air as it is, so as to be cooled. , Japanese--After putting the inoculum into the culture tank 30, when inoculating the inoculum, send in *

490286 V. Description of the invention (ίο) The gas-inspired tube 4 2 ′ is inflated from the rim portion 4 2 a for cultivation. By this aeration, the mycelium can be uniformly cultured on the entire culture solution. Apply a little pressure when inflating. The adjustment of the air pressure can be performed by the valves 45 and 52, and the amount of inflation can be controlled by the adjustment of the on-off valve 48. As described above, since the culture tank 30 of this embodiment can be constructed simply, the device 'can reduce its production cost and can be easily produced. Compared with the structure in which the culture liquid is sterilized in the culture sample, the culture tank 30 of this embodiment has a significantly reduced operating cost. ： 'System and using the culture tank 30 for liquid culture of seed bacteria, compared to the case of cultivating Pleurotus edodes mushrooms during the cultivation of seed bacteria and other seed bacteria in about 25 ~ 30 days, # f liquid culture, only 7 ~ 8 days to complete the cultivation, Therefore, the borrowing period can be greatly shortened. Compared with the case where the cultivation period of sawdust seed bacteria is long and the large cultivation space is required due to the relationship between the two species, the use of the cultivation tank can equal the accumulated space. β Huanlang Province Also, indoor air-conditioning equipment is required for sawdust seed bacteria, but to use culture, only room temperature adjustment equipment is needed without using air-conditioning equipment. It is necessary to cultivate the bacteria and other bacteria in the large culture room. The results of different breeding results are different when the location of the culture room is different. However, when using a culture tank, the entire pull = the upper reel is uniformly elongated without causing uneven culture results. The advantages. (Liquid inoculation method for species ii) = The body 32 and the liquid inoculation device are in communication with each other through a connecting pipe 56 provided on the bottom surface. 58 is an on-off valve. The inoculation operation of the susceptor 32 j bacteria was performed in a state where the culture tank 30 was connected to the culture tank 30 through the connection tube 56. Fig. 5 砉 + 丝 # The gift is connected to the master of the device under the inoculation state of Leyuan

490286 V. Description of the invention (11) The composition of the main part. The inoculation of the inoculum is to spray a certain amount of liquid inoculum to the mouth of the cultivation bottle filled with the cultivation material. The opening and closing of the dispersion valve is supplied; the nozzle 60 ′ is supplied with air and dust added to the culture tank 30 and supplied from the culture tank 30 囷 囷. In order to adjust the spread amount of liquid seed bacteria, in this embodiment, the opening and closing time of the air valve 62 is controlled by the setting of "off 64", thereby adjusting the setting of the two-time switch of the air. . Culture tank 3. The middle side is inflated,-thank you for giving = Figure 6 is a schematic diagram showing the method of inoculating liquid seed bacteria in a bottle 68 stored in a container 66. In the Taguchi delivery cabinet 66 of this embodiment, liquid inoculum was inoculated to each row at the same time. =: Cultivation, 68 bottles with the same number of nozzles 6. Passed through each cultivation bottle 68; standing; Fang: quantitatively inoculate the liquid seed bacteria on the mother-row cultivation bottle 68. As shown in the figure, the rows of cultivation bottles 68 are inoculated with liquid seed fungus, and the cultivation bottles 6 8 are inoculated with energy. Γ rabbit potential, ~ is the first row of Zhuang &-Γ Τ The four-row cultivation bottles 68 can be inoculated in a row of ^ H row inoculation, which can inoculate the whole plant ^ liquid seed bacteria contained in the cargo hub 66. … For the container No. 2 that has been inoculated with liquid seed bacteria, the number of nozzles at 6 ° is selected. When inoculating the liquid seed bacteria with <application, 'the majority of rows of cultivation bottles can be inoculated with liquid seed bacteria at the same time' Container 66 is inoculated with liquid seed bacteria. ^ ^ Preparation of liquid inoculum inoculation for one person, fill the cultivated bottle 68 with adjusted water content (mine five, invention description (12)), and fill the cultivated material with the same inoculation This is similar to the traditional appearance of using sawdust seed fungi and inoculated with liquid seed fungus F | β G &quot; fe k # V · body seed fungus. In the absence of ^, you must open the mule 69 to inoculate the liquid flute 7Λ ..., 7 ^ It is closed in the same way as traditional methods. to make.加加 7, 2 ^ shows an embodiment of the liquid seed fungus inoculation device. The removal mechanism 74 is provided with a conveyor belt 70 in the elongation direction, and a cover 69 for the liquid 69 above the conveyor belt 70. 76 is a storage device. Nozzle 60 for inoculating fluid limbs and body parts such as air valves. The eighth example, A too 8!? It is not used in the nozzle 60 of the liquid inoculum inoculation device, and is matched with the arrangement position of the cultivation bottle 68 in the container 66. There are 4 nozzles 60 fixed to the nozzle to support the fine placement ^ ^^ ^ ^ f 82, ^ δβ w Λ, \ t〇 The connecting part on the rear side is connected to a tube 86 which communicates with the culture tank 30. When the genital type =, it is necessary to use the nozzle 6. Stop and lock on the frame, there is a "like: card can be cleaned and disinfected ..." The head of the inoculated inoculum inoculation machine is also described below. How to make the result of cultivation of Pleurotus edodes by the above method. Liquid rainbow seed exterminator application Table 1 is a table of the liquid seed fungus cultured using the conventional method for cultivation of the ore seed fungus. Comparison of the results of P. chinensis mushrooms The volume of the cultivation bottle used was 1100 cc, and the culture bottle filled with the cut culture bottle was 490286. 5. Description of the invention (13): ears of corn, rice bran, fermented soybeans, etc. The grounding machine is an open-hole type 5 pieces. The test counts of the cultivation bottles The conventional sawdust and liquid seed fungi were 16 000 sticks per day when filled for 4 days, and the total counts were 6 400 sticks. Conventional sawdust seed fungus cultivation facilities were used, and sawdust seed fungus and liquid seed fungus were grown under the same cultivation conditions. Table 1 Items · Liquid seed bacterial inoculation Sawtooth bacterial inoculation time / 1600 sticks 13 points 22 points mycelium alive 2 to 3 days 7 to 10 days Choking is completely absent 37 people who seem to have problems are mixed with miscellaneous products No 153 are confirmed at all 20 to 22 days of cultivation is completed. 28 to 33 days. Buds are neat and apparently uneven. Paper roll operation can be performed together during the paper roll period. Paper rolls must be individually selected at the appropriate time. The length of the side filaments is extended without delay. Good traditional yield. 303 ~ 392g Average 337g 264 ~ 358g Average 285g Quality shape 'Umbrella-shaped, no drying problem There are water-like garlic. Table 1 summarizes the significant differences between those who are familiar with traditional sawdust bacteria and those who use liquid bacteria. The use of liquid inoculum can reduce the time for which mycelium is alive and the number of days it takes to complete the culture. In addition, it takes 29 days to complete the cultivation to harvest days, regardless of whether sawdust or liquid inoculum is used. This is because the number of days from the mycelium alive to the cultivation process is shorter when using liquid inoculum.

Page 16 490286 V. Description of the invention (14) After comparing the cultivation process and the quality of mushrooms, it can be used to roll paper together under extremely stable conditions. It is excellent for the use of liquid seed bacteria. At the same time, the average yield was 337g, and the yield was 258g, which increased greatly. The use of liquid has also increased, with considerable effects. The use of such liquid inoculum is not only added, but also has the advantages of uncovering the same fashion. In other words, the product is evenly produced. Due to harvest time rate. The use of liquid inoculum can shorten the inoculation of many inoculums. At the same time, continuous inoculation is necessary depending on the cultivation of cultivars. By inoculation of seed bacteria. It is also possible to make germ halide. It is not easy to produce seed fungus changes and seed fungus quality. On the other hand, liquid inoculum was cultivated. The efficiency can also improve the elongation of the side filaments. It is better than the conventional use of sawdust bacteria. The quality is improved when there is no sprouting and neatness. The quality is also high. The harvest of the seed bacteria is improved, and the quality of the harvest is improved. Available time. Since the size of the cultivatable tank can be simplified with liquid seed bacteria, or it is not easy to age, and the yield can also increase the quality without improving the operation efficiency. How can the number of seeds be fully automated? This can ensure that 490286 Brief description of the diagram. Figure 1 is an explanatory diagram of the process of preparing the inoculum used in the pre-cultivation; Figure 2 is an explanatory diagram of the process of cultivating the inoculum in the pre-culture; Figure 3 is an explanatory diagram of the cultivation process of the liquid inoculum; The figure is a structural explanatory diagram of a culture tank; FIG. 5 is a block diagram showing a method for inoculating liquid seed bacteria; FIG. 6 is an explanatory diagram showing a method for inoculating liquid seed bacteria in a cultivation bottle; FIG. 7A is a front view of an inoculation device; FIG. 7B The figure is a side view of the inoculation device; FIG. 8A is a front view of the nozzle of the inoculation device; and FIG. 8B is a side view of the nozzle of the inoculation device. &lt; Names and symbols of components in the diagram &gt; 10 test tubes 12 flat plates ground 18 cut pieces 20 containers 30 cultivation tanks 32 slots body 33 casters 34 access V36, 37 • inspection 38: open and close cover 40

Page 18 490286

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Claims (1)

490286 fix (Ό) The culture process of the liquid seed bacteria used in the liquid culture method for the culture of the seed bacteria obtained from the culture of the fungus beads and liquid culture method, and &lt; the cost of case number 89120513 6. Application for patent scope 1. Liquid seed bacteria of Citron I The manufacturing method includes: mixing the liquid seed bacteria prepared in the pre-cultivation process with one another; b ,, A, see 7 in a culture tank containing a seed bacteria nutrient solution; During the cultivation process, the entire culture tank of t σ is contained in a high pressure After harvesting the culture liquid in the culture tank, the bactericidal solution is autoclaved, and the autoclaved culture liquid is cooled to normal temperature. After the culture liquid is cooled, the liquid prepared in the previous cultivation process is seeded in the culture. The culture solution in the tank is subjected to the present culture. Ugly 2 · The method for producing a liquid seed fungus according to item 1 of the patent application scope, wherein the culture liquid is a soybean powder and sugar added to the steaming water. 3. The manufacturing method of liquid seed bacteria as described in the first item of the patent scope of Shenyin, wherein the culture solution is allowed to cool to normal temperature during the culture process. The air used for the removal of germs was introduced into the culture tank through the device. Working gas 4 · The manufacturing method of liquid seed bacteria according to item 1 of the patent application scope, wherein the tank system of the culture tank forms a closed container, and the standing tank is provided with a tube for introducing air for inflation. Installer by spitting out empty ^ from the bottom of the container. I * 1 5 · As in the method for manufacturing liquid inoculum of item 1 of the scope of patent application, the culture tank used therein has an air input portion connected to the tank body via an on-off valve and an air filter. Adjust &amp; 490286 Case number 89120513 Scope of patent application 6 · — A kind of mushroom mushroom carving for inoculating liquid inoculum in cultivated ground — The device is provided with an inoculation section for inoculating liquid inoculum in cultivated ground. The nozzle, and the cloth control the opening and closing time of the nozzle, by adding the control of the amount of liquid seed bacteria supplied from the culture tank / 卩 θ, lice firm control 7. The liquid seed bacteria such as the scope of the patent application No. 6 The nozzle of the inoculation 2 is provided with a timer, which controls the liquid inoculum from the nipple, and the control part is provided with a timer that controls the air sent to the air valve to the door. When the industrial milk valve is opened and closed, Λ t ί ask the container transport mechanism of the inoculation of the dream bottle for liquid seed bacteria in item 6 or 7 of the patent scope, and the goods in the cultivation place with a land; The inoculation part that is provided with the nozzle is extended to the liquid inoculum inoculation 9 = shen-decay two cultivation bottles. The inoculation device for liquid inoculum of item 8 in the scope of 沭 a IZ, the double-quantity rotation mechanism is to set the cargo right so that it can be placed in each row of cultivation bottles: ΪΪ: cooperate with each row of cultivation bottles contained in the container Share configuration bit