TW260624B - Process of separating tryptophan (Trp) from zymotic fluid by ion-exchange displacement chromatography - Google Patents
Process of separating tryptophan (Trp) from zymotic fluid by ion-exchange displacement chromatographyInfo
- Publication number
- TW260624B TW260624B TW81106505A TW81106505A TW260624B TW 260624 B TW260624 B TW 260624B TW 81106505 A TW81106505 A TW 81106505A TW 81106505 A TW81106505 A TW 81106505A TW 260624 B TW260624 B TW 260624B
- Authority
- TW
- Taiwan
- Prior art keywords
- zymotic fluid
- ion
- tryptophan
- displacement chromatography
- trp
- Prior art date
Links
Abstract
A process of separating and purifying tryptophan (Trp) from zymotic fluid by ion-exchange displacement chromatography, which comprises: (a) adjusting a zymotic fluid, which comprises 5.55~12 g/l of tryptophan and 30~40 g/l of ammonium ion, to pH of 3~4 and temperature of 20 deg.C~40 deg.C followed by pre-treatment of sedimentation and centrifugation so that the zymotic fluid comprises 5.55~11.69 g/l of tryptophan and 10~40 g/l of ammonium ion, adjusting the zymotic fluid to pH of 3~4 and temperature of 25 deg.C~35 deg.C; (b) injecting the zymotic fluid into a H-type ion-exchange resin column which contains particles at size of 5 mm ~ 600 mm and is at pH of 2~4; (c) using purified water to perform back washing in order to flush out un-adsorbed zymotic fluid retained in the column; and (d) using ammonia solution with concentration of 0.92~1.13 g/l to undergo displacement chromatography at space velocity of 5~10 ll-1hr-1.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
TW81106505A TW260624B (en) | 1992-08-18 | 1992-08-18 | Process of separating tryptophan (Trp) from zymotic fluid by ion-exchange displacement chromatography |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
TW81106505A TW260624B (en) | 1992-08-18 | 1992-08-18 | Process of separating tryptophan (Trp) from zymotic fluid by ion-exchange displacement chromatography |
Publications (1)
Publication Number | Publication Date |
---|---|
TW260624B true TW260624B (en) | 1995-10-21 |
Family
ID=51401870
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
TW81106505A TW260624B (en) | 1992-08-18 | 1992-08-18 | Process of separating tryptophan (Trp) from zymotic fluid by ion-exchange displacement chromatography |
Country Status (1)
Country | Link |
---|---|
TW (1) | TW260624B (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
TWI691592B (en) * | 2009-06-04 | 2020-04-21 | 美商奇諾麥提卡公司 | Process of separating components of a fermentation broth |
-
1992
- 1992-08-18 TW TW81106505A patent/TW260624B/en not_active IP Right Cessation
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
TWI691592B (en) * | 2009-06-04 | 2020-04-21 | 美商奇諾麥提卡公司 | Process of separating components of a fermentation broth |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
MM4A | Annulment or lapse of patent due to non-payment of fees |