FR2423987A1 - Cytochrome C and protein prodn. from yeast - by lysis followed by ion exchange chromatography - Google Patents

Cytochrome C and protein prodn. from yeast - by lysis followed by ion exchange chromatography

Info

Publication number
FR2423987A1
FR2423987A1 FR7812563A FR7812563A FR2423987A1 FR 2423987 A1 FR2423987 A1 FR 2423987A1 FR 7812563 A FR7812563 A FR 7812563A FR 7812563 A FR7812563 A FR 7812563A FR 2423987 A1 FR2423987 A1 FR 2423987A1
Authority
FR
France
Prior art keywords
cytochrome
prodn
yeast
proteins
protein
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
FR7812563A
Other languages
French (fr)
Other versions
FR2423987B1 (en
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
VINCHON GEORGES
Original Assignee
VINCHON GEORGES
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by VINCHON GEORGES filed Critical VINCHON GEORGES
Priority to FR7812563A priority Critical patent/FR2423987A1/en
Priority to PCT/FR1979/000036 priority patent/WO1979000993A1/en
Priority to DE19792948827 priority patent/DE2948827A1/en
Priority to GB7943310A priority patent/GB2036752A/en
Priority to JP50071479A priority patent/JPS55500315A/ja
Publication of FR2423987A1 publication Critical patent/FR2423987A1/en
Application granted granted Critical
Publication of FR2423987B1 publication Critical patent/FR2423987B1/fr
Granted legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/005—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor after treatment of microbial biomass not covered by C12N1/02 - C12N1/08
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
    • A23J1/00—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
    • A23J1/18—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from yeasts
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/795—Porphyrin- or corrin-ring-containing peptides
    • C07K14/80—Cytochromes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/06—Lysis of microorganisms
    • C12N1/063—Lysis of microorganisms of yeast

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Mycology (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biophysics (AREA)
  • Sustainable Development (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Prodn. of haemoproteins (esp. cytochrome C) and proteins from yeasts comprises (a) subjecting the yeast to cellular lysis in a liq. medium under pressure; (b) purifying the lysate by precipitating nucleic acids (e.g. with MnCl2) and salting out cell residues (e.g. with NH4 sulphate); (c) adjusting the purified lysate to pH 8-8.5 and passing it over an ion-exchange resin to adsorb the cytochrome; (d) adjusting the effluent to pH 3.3-4 and precipitating proteins with (NH4)2SO4; (e) washing the resin with an aq. alkaline soln. with a pH of 8-8.25; and (f) eluting the cytochrome with an aq. soln. of (NH4)2SO4. The process gives high yields (e.g. 250 g/tonne) of high-purity cytochrome C and high yields (e.g. 340 g/tonne) of high-purity proteins suitable for use in human and animal nutrition.
FR7812563A 1978-04-27 1978-04-27 Cytochrome C and protein prodn. from yeast - by lysis followed by ion exchange chromatography Granted FR2423987A1 (en)

Priority Applications (5)

Application Number Priority Date Filing Date Title
FR7812563A FR2423987A1 (en) 1978-04-27 1978-04-27 Cytochrome C and protein prodn. from yeast - by lysis followed by ion exchange chromatography
PCT/FR1979/000036 WO1979000993A1 (en) 1978-04-27 1979-04-25 Process for preparing hemoproteins and proteins from a yeast and products obtained thereby
DE19792948827 DE2948827A1 (en) 1978-04-27 1979-04-25 PROCESS FOR PREPARING HEMOPROTEINS AND PROTEINS FROM A YEAST AND PRODUCTS OBTAINED THEREBY
GB7943310A GB2036752A (en) 1978-04-27 1979-04-25 Process for preparing hemoproteins and proteins from a yeast and products obtained thereby
JP50071479A JPS55500315A (en) 1978-04-27 1979-04-25

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR7812563A FR2423987A1 (en) 1978-04-27 1978-04-27 Cytochrome C and protein prodn. from yeast - by lysis followed by ion exchange chromatography

Publications (2)

Publication Number Publication Date
FR2423987A1 true FR2423987A1 (en) 1979-11-23
FR2423987B1 FR2423987B1 (en) 1980-10-31

Family

ID=9207679

Family Applications (1)

Application Number Title Priority Date Filing Date
FR7812563A Granted FR2423987A1 (en) 1978-04-27 1978-04-27 Cytochrome C and protein prodn. from yeast - by lysis followed by ion exchange chromatography

Country Status (1)

Country Link
FR (1) FR2423987A1 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2509139A1 (en) * 1981-07-09 1983-01-14 Weston George Ltd MICROBIAL PROTEIN ISOLATION WITH REDUCED NUCLEIC ACID CONTENT

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2113190A5 (en) * 1970-10-28 1972-06-23 Hitachi Ltd

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2113190A5 (en) * 1970-10-28 1972-06-23 Hitachi Ltd

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2509139A1 (en) * 1981-07-09 1983-01-14 Weston George Ltd MICROBIAL PROTEIN ISOLATION WITH REDUCED NUCLEIC ACID CONTENT

Also Published As

Publication number Publication date
FR2423987B1 (en) 1980-10-31

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