FR2424319A1 - Cytochrome C and protein prodn. from yeast - by lysis followed by ion exchange chromatography - Google Patents

Cytochrome C and protein prodn. from yeast - by lysis followed by ion exchange chromatography

Info

Publication number
FR2424319A1
FR2424319A1 FR7812562A FR7812562A FR2424319A1 FR 2424319 A1 FR2424319 A1 FR 2424319A1 FR 7812562 A FR7812562 A FR 7812562A FR 7812562 A FR7812562 A FR 7812562A FR 2424319 A1 FR2424319 A1 FR 2424319A1
Authority
FR
France
Prior art keywords
cytochrome
prodn
yeast
proteins
protein
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
FR7812562A
Other languages
French (fr)
Other versions
FR2424319B1 (en
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
VINCHON GEORGES
Original Assignee
VINCHON GEORGES
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by VINCHON GEORGES filed Critical VINCHON GEORGES
Priority to FR7812562A priority Critical patent/FR2424319A1/en
Priority to GB7943310A priority patent/GB2036752A/en
Priority to JP50071479A priority patent/JPS55500315A/ja
Priority to PCT/FR1979/000036 priority patent/WO1979000993A1/en
Priority to DE19792948827 priority patent/DE2948827A1/en
Publication of FR2424319A1 publication Critical patent/FR2424319A1/en
Application granted granted Critical
Publication of FR2424319B1 publication Critical patent/FR2424319B1/fr
Granted legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/795—Porphyrin- or corrin-ring-containing peptides
    • C07K14/80—Cytochromes

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Prodn. of haemoproteins (esp. cytochrome C) and proteins from yeasts comprises (a) subjecting the yeast to cellular lysis in a liq. medium under pressure; (b) purifying the lysate by precipitating nucleic acids (e.g. with MnCl2) and salting out cell residues (e.g. with NH4 sulphate); (c) adjusting the purified lysate to pH 8-8.5 and passing it over an ion-exchange resin to adsorb the cytochrome; (d) adjusting the effluent to pH 3.3-4 and precipitating proteins with (NH4)2SO4; (e) washing the resin with an aq. alkaline soln. with a pH of 8-8.25; and (f) eluting the cytochrome with an aq. soln. of (NH4)2SO4. The process gives high yields (e.g. 250 g/tonne) of high-purity cytochrome C and high yields (e.g. 340 g/tonne) of high-purity proteins suitable for use in human and animal nutrition.
FR7812562A 1978-04-27 1978-04-27 Cytochrome C and protein prodn. from yeast - by lysis followed by ion exchange chromatography Granted FR2424319A1 (en)

Priority Applications (5)

Application Number Priority Date Filing Date Title
FR7812562A FR2424319A1 (en) 1978-04-27 1978-04-27 Cytochrome C and protein prodn. from yeast - by lysis followed by ion exchange chromatography
GB7943310A GB2036752A (en) 1978-04-27 1979-04-25 Process for preparing hemoproteins and proteins from a yeast and products obtained thereby
JP50071479A JPS55500315A (en) 1978-04-27 1979-04-25
PCT/FR1979/000036 WO1979000993A1 (en) 1978-04-27 1979-04-25 Process for preparing hemoproteins and proteins from a yeast and products obtained thereby
DE19792948827 DE2948827A1 (en) 1978-04-27 1979-04-25 PROCESS FOR PREPARING HEMOPROTEINS AND PROTEINS FROM A YEAST AND PRODUCTS OBTAINED THEREBY

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR7812562A FR2424319A1 (en) 1978-04-27 1978-04-27 Cytochrome C and protein prodn. from yeast - by lysis followed by ion exchange chromatography

Publications (2)

Publication Number Publication Date
FR2424319A1 true FR2424319A1 (en) 1979-11-23
FR2424319B1 FR2424319B1 (en) 1980-10-31

Family

ID=9207678

Family Applications (1)

Application Number Title Priority Date Filing Date
FR7812562A Granted FR2424319A1 (en) 1978-04-27 1978-04-27 Cytochrome C and protein prodn. from yeast - by lysis followed by ion exchange chromatography

Country Status (1)

Country Link
FR (1) FR2424319A1 (en)

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS4818825B1 (en) * 1970-03-27 1973-06-08

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS4818825B1 (en) * 1970-03-27 1973-06-08

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
CA1966 *
CA1973 *

Also Published As

Publication number Publication date
FR2424319B1 (en) 1980-10-31

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