TW205504B - - Google Patents

Description

Printed by the Beigong Consumer Cooperative of the Bureau of Standards and Economics of the Ministry of Economic Affairs ^ 05504 A6 _B6__ V. Description of the Invention (1) This case was proposed on October 30, 1990, and is incorporated by reference in the US Patent Application No. 07 / 605,899 A forced case. 1. Ziming: > EU City The present invention is developed for products for hygienic use of women, such as lavage fluids, plugs, gels, lotions, and contraceptives. The present invention particularly refers to an effective ingredient which, when combined with feminine hygiene products, will reduce the amount of certain toxins produced by bacteria. 2. Hair loss Women use many women's hygiene and internal cleaning products, mainly in liquid form. More specifically, many women use vaginal lavage fluid to inject and clean the vagina, and to prevent vaginal infections, for contraception, sterilization, and to promote miscarriage (see THOMAS A. Gossel's "Women's Hygiene Products • Why You Opinions ", American Pharmacist, May 1986, pages 20-27). The components of vaginal lavage can be composed of various different components. Vinegar is the most common substance used for cleaning vaginal lavage. Vinegar contains about 4-6¾ acetic acid. However, there is not enough data to decisively prove that vinegar effectively changes the vaginal Ph for a sufficient length of time to promote normal vaginal plexus growth and thereby prevent Qi infection. Published on November 13, 1974, British Patent Specification No. 1374, 105, titled "Effervescent Ingredients", describes vaginal lavage ingredients containing silicone. These ingredients can be made into tablets, and used as gold deputy denture cleaner, antacid, analgesic laxative and vaginal lavage. The patented ingredients in this patent include two gasification sulfonates and / or gas-generating materials, such as persulfuric acid (please read the precautions on the back before writing this page). Packing and ordering Taizhao chromium K 疳 ϋί 丨 II Ψ Mission B3 Zhaijinjin (CNS) V4 Sige (21 (1x297)) Λ 6 13 6 V. Description of invention 2 [) Toilet / perborate mixture, as well as the best medicines, diluents, for example, sodium nitrate, Integrating agents such as ethylenediaminetetraacetic acid, surfactants, lubricants, fragrances and odors. US Patent No. 2,584,119 issued to Diniel B. Langley on June 8, 1971 describes vaginal lavage ingredients, containing 3-8 parts by weight of water-soluble detergent, sodium dodecylbenzenesulfonate, potassium or sodium lauryl sulfate , Or 2-5 parts by weight of age-specific monopersulfate or 5-10 parts by weight of radon metal or borate, used as an aqueous solution of 5-60 grams per liter. U.S. Patent No. 3,219,525 issued to Samuel G. Berkow et al. On November 23, 1965 describes a pressurized container in which there is a solution containing 1.5 to 2.5 grams of cationic quaternary ammonium bactericidal surface active 30-60 grams of antiseptic wetting agent. The resulting component is an aerosol foam. It has been reported that daily lavage with acidic or alkaline solutions does not cause generalized phlegm or vaginal mucosa in the vagina. It has also been directed and proved that during lavage, the vaginal Ph presents the Ph of the lavage solution. Thirty minutes after lavage with an acidic solution, Ph actually becomes age-appropriate. Printed by the Employee Consumer Cooperative of the Central Standards Bureau of the Ministry of Economic Affairs (please read the precautions on the back before filling out this page) line *

Strobino et al. Reported that some lavage agents are toxic to sperm and are therefore used as a contraceptive. Conversely, sodium bicarbonate lavage is another type of lavage used to improve sperm survival and thus enhance fertility (see An sari, Gould and Ansari, "Na nitrate lavage is used to improve sexual intercourse "Experiment", Fertility and Infertility, Volume 33, Issue 6, June 1980, pages 608-612). -4-This paper uses the Chinese National Standard (CNS) A4 specifications (210X297 public waste) Λ 6 η 6 V. Description of invention 3 () Toxic Shock Syndrome (TSS) It is a heavy and sometimes fatal multi-lineage disease. This type of disease is associated with Staphylococcus aureus infection and colonization. This disease is related to the use of tampons during menstruation. This disease is caused by the toxic shock withdrawal group toxin (T 〇> {丨 c S h 〇ck S yndr 〇me Τ ο X i η -1, referred to as TSST-1), the toxin is caused by menstrual TSS Most «§ Staphylococcus species isolated from patients. Printed by the Employee Consumer Cooperative of the Bureau of Standards of the Ministry of Economic Affairs (please read the notes on the back before filling out this page) Ding published a report on the toxic shock group and the use of tampons. Several researchers have conducted research. The purpose is to estimate the effect of tampons on the growth of Staphylococcus aureus and the effect of tampons on TSST-1 produced by the bacteria. Researchers are committed to the role of tampons in TSS, and these early efforts have produced conflicting data. Schlievert et al. (Obstetrics and Gynecology, Vol. 64, pages 666-670, November 1984) studied the effects of tampons on Staphylococcus aureus to assess whether the components of tampons increased the growth of S. aureus And the production of toxic shock syndrome group toxin-1. They concluded that, under the experimental conditions of their research, the components of the tampon had neither nutrients for the growth of toxic shock staphylococcus aureus nor caused the production of toxic shock staphylococcus toxin-1 higher than the control Factors of standard. After the six-hour incubation period, several commercially available tampons tested had inhibitory effects on bacterial growth and had suppressed toxin production. Other tampons suppress toxin production but do not inhibit cell growth. A tampon inhibits cell growth but increases the amount of toxins produced. On the other hand, Tierno and this paper standard use the Chinese National Standard (CNS) A4 specifications (210x297 g; ¢) Λ 6 Β6 V. Invention description (4)

Hanna (Contraception, Volume 31, pages 185-194, 1985) reported that in its test, the tampons had promoted the production of TSST-1 in golden grapes.

Reiser et al. (Journal of Clinical Withdrawal of Biology, Volume 25, Issue 8, 1450-1452 pages, August 1987) subsequently reported the results of these trials, which were conducted to determine the four brands of tampons in production The role of toxic shock and toxin-1. The existing air volume of the tampon subjected to the test is limited to what is contained in the bladder (the bladder is made of a cellulose chart shell with a molecular weight reduced to less than 10,000). The tampon is encapsulated during the test. This method is considered to be advantageous because the limited amount of existing air is more compact than the previously used method and simulates the internal body condition of the vaginal tampons during menstruation, and because the tampon under test is tested before the test No change. Reise "The results of experiments conducted by others pointed out that cotton plugs increase the surface area of Staphylococcus aureus growth and provide sufficient gas for toxin production. The report did not mention that any cotton plugs tested had a significant effect on the growth of staphylococcus aureus or TSST-1 production. Inhibition effect. Printed by the Ministry of Economic Affairs, Bureau of Standards, Employee Consumer #Cooperative (Please read the precautions on the back before writing this page) Ding

Robbins et al. (Published in the Journal of Clinical Microbiology, Volume 25, No. 8, pages 1446-1449, August 1987) and Reiser et al. Reported the use of disk-membrane-agra (DMA for short) Method, under the condition of 5% CO2 in the air, incubating at 39 ° C for 19 hours, the effect of 17 commercially available cotton tampons on TSST-1. Spread the filter-covered agar medium (with or without blood) in a small flat petri dish and inoculate it with a strain of Staphylococcus aureus producing TSST-1. Robbins et al inferred that the cotton paper scale used the Chinese National Standard (CNS) A4 specifications (210x297 public waste)

5. Description of the invention (5) The main function of the plug in TSS may be to provide the fiber surface for large-scale wedge colonization, and to provide enough air for TSST-1 to produce. In addition, they found that an additive such as a deodorant / surfactant used in a commercial deodorant suppresses TSST-1 production, and, as seen in a case of a different commercially available tampon, by suppressing the golden grape ball Evidence for growth of scrofula to reduce TSST-1 production. Inhibition of TSST-1 production and inhibition of staphylococcus aureus growth are believed to prove important in reducing the risk of TSS. S. Notermans et al. (Journal of Food Safety, Volume 3, 1981, pages 83-88) reported that glycerol monolaurate had inhibited the brain when used at a rate of 5 grams per kilogram of meat slurry (Ph 6.0-6.2) Toxin production caused by Enterobacterium type A, B and E. This article does not mention Staphylococcus aureus, or any toxins produced therefrom, nor does it mention feminine hygiene ingredients or toxic shock phenotypes using glycerol monolaurate. Printed by the Employee Consumer Cooperative of ΨCentral Standards Bureau of the Ministry of Economic Affairs (please read the precautions on the back before writing this page) Line 'In the Toxic Shock Bullying Group (TSS), regardless of whether it has menstrual symptoms with menstruation, symptoms include fever High blood pressure, skin rash, and peeling. TSST -1 is highly correlated with menstrual cases, but in non-menstrual cases it is less common to isolate this toxin from Staphylococcus aureus. Because TSST-1 may cause many clinical features of TSS in rabbits and other animals, it is generally considered to be the virin of TSS (Schlievert, "Staphylococcal Enterotoxin B and Toxic Shock Syndrome Toxin-1 are not TSS has significant association ", Lancet, Volume 1 (8490), May 17, 1986). However, Garbe (Garbe, Arko, Reingold et al., "From Non-menstrual Poisoning-7-This paper scale uses the Chinese Family Standard (CNS) A 4 specifications (210 > < 297 public) Central Standards Bureau of the Ministry of Economic Affairs Printed by β Industry Consumer Cooperative Society Λ 6 ___ Β_6_ V. Description of the invention (6) Staphylococcus aureus isolated from patients with sexual shock syndrome: evidence of additional toxins, Journal of the American Medical Association, 1985, Vol. 253, pages 2538-42 ) Mentioning many TSSs isolated from non-menstrual cases does not mean TSST-1, although it causes rabbit-like TSS symptoms. Among the toxins formed by non-menstrual isolates of Staphylococcus aureus, TSST-1 was produced by SchlievertA reporter 40¾. Staphylococcus aureus produces TSST-1, and most people associate menstrual TSS with the use of tampons. We have embarked on experiments to determine whether the production of TSST-1 can be minimized or discontinued within the absorbent fiber material. Unexpectedly, I discovered a group of compounds described in the pending US Patent Application No. 343,965 filed on April 27, 1989 and 3,742 filed on April 27, 1990 (both are referenced and incorporated herein) . However, we need to find a lavage ingredient that can restrain TSST-1 production by Staphylococcus aureus in the vaginal cavity. Invention Xiyashu The present invention relates to non-absorbable ingredients for vaginal rinsing, which contains a compound selected from the following group: (a)-a polyhydroxy aliphatic alcohol and a fatty acid containing eight to eighteen atoms Monoesters, in which at least one hydroxyl group of the monoester is associated with the residue of aliphatic alcohol; (b) a polyhydroxy aliphatic alcohol and a diester of fatty acids containing eight to eighteen atomic atoms, Among them, the diesters have at least one hydroxybook paper, and the Chinese National Standard (CNS) A4 specifications (210X297 public goods) are used (please read the precautions on the back before writing this page). The Central Standards Bureau of the Ministry of Economic Affairs A Printed by the Industry and Consumer Cooperatives Λ 6 ___ \ i 6 V. Description of the invention (7) The group is associated with the aliphatic alcohol residue, and (c) a mixture of the above monoesters and diesters. The fatty acid parts of the above monoesters and diesters can be obtained from caprylic acid, capric acid, lauric acid, myristic acid, palmitic acid, and stearic acid. The lengths of these acid bonds are Ce, Cio, and Cl2, respectively , C | 4, Cte and Cl8 saturated fatty acids. The fatty acid parts of the monoesters and diesters mentioned above can also be obtained from unsaturated fatty acids with a length ranging from c8 to c18. Oleic acid is an example of such unsaturated fatty acids. The preferred fatty acid used in the practice of the present invention is lauric acid, and its chemical formula is a saturated fatty acid of Cu H23 C00H. The term "aliphatic" in this specification and the appended patent application usually means the same as organic chemistry, that is, "aliphatic" refers to the special sulfonic atom as a straight chain or branched chain. The organic compounds that are arranged. The term "polyhydroxy" in the scope of the patent application attached to this description refers to the presence of at least a dihydroxy (OH) group in the compound. Therefore, a polyhydroxy aliphatic alcohol is a kind of at least dihydroxy In addition, the sulfonic framework is a straight or branched aliphatic alcohol. The polyol suitable for forming monoesters and / or diesters for the implementation of the present invention is 1,2-ethylene glycol; 1, 2, 3 -Glycerin (glycerin); 1,3-propanediol; 1,4-butanediol; 1,2,4-butanetriol and the like. Used to form monoesters and diesters for the implementation of this The invented polyhydric aliphatic alcohol is 1,2,3-glycerol (commonly known as glycerin), and its chemical formula is H0CH2CH (0H) CH20H. -9-This paper scale uses the Chinese National Standard (CNS) A4 specifications ( 210x297 public goods) (Please read the precautions on the back before filling out this page) Outfit · Printed by the Ministry of Economic Affairs, Standards, and Consumers ’Cooperative Society Λ 6 Π 6 5 Description of the invention (8) The esters which can be used to implement the present invention have at least one hydroxyl group associated with the aliphatic alcohol residue. Therefore, please understand that the monoester of 1,2-ethylene glycol and one of the aforementioned fatty acids can be used For implementing the present invention, because the general formula is

The above-mentioned ester of CnH2n + 1-C-0-CH2-CH20H 0 has at least one hydroxyl group (that is, the hydroxyl group on the far right side of the structural formula shown above) in the part of the ester obtained from the aliphatic alcohol 1,2-glyoxan . On the other hand, please understand that 1,2-ethylene glycol diester and one of the above fatty acids cannot be used in the practice of the present invention because the general formula is

CnH2n + l_C ~ 0-CH2 * CH20 ~ C-CnII2n + l 0 The above-mentioned ester does not have at least one hydroxyl group in the ester obtained from 1,2-ethylene glycol part 0 Monoglyceride and one of the specified fatty acids can be used for The present invention is practiced because the ester will have two hydroxyl groups derived from glycerin associated with it. Diglycerides and one of the specified fatty acids can also be used because the ester will have a hydroxyl group derived from the aliphatic alcohol glycerol associated with it. Indeed, as will be understood below, blends of glyceryl monolaurate and glyceryl dilaurate have been found to be useful in the practice of the present invention. Finally, please understand that the triglyceride and one of the specified fatty acids cannot be used to implement the present invention, because the vinegar is less than -10-this paper scale is used in China National Standard (CNS) A4 specifications (210X297 public goods) ( Please read the precautions on the back first and then fill out this page) Outfit · Printed by the quasi-bureau of the Ministry of Economic Affairs KX Gongxiaot Cooperative Society 0 · ': ί Λ 6 ______ η 6__ V. Description of the invention (9)

Less than one hydroxyl group in part D obtained from fatty alcohols (ie glycerol). Preferred esters for use in the practice of the invention are glyceryl monolaurate, glyceryl dilaurate and mixtures thereof. Particularly preferred is glyceryl-laurate sold under the trade name " Monomuls 90 L-12 " by Henke Company. This compound contains about 96 «glyceryl-laurate by weight. It is used in the following examples No glyceryl dilaurate was detected in this compound sample. Glyceryl monolaurate is a compound listed by the Food and Drug Administration (FDA) GRAS for use as a food emulsifier. This material is non-toxic and resistant to humans Microorganisms. Other preferred esters for use in accordance with the present invention include C-3 alkanol-laurate derivatives, such as 2-hydroxy-propyl laurate and 3-hydroxy-1-propyl laurate It is expected that the dilaurates of C-3 alkanols such as glyceryl-1,3-dilaurate, glyceryl-1,2-dilaurate also reduce the production of enterotoxins A, B, C and The amount of TSST-1 for enterotoxin A. Ethylene glycol derivatives such as ethylene glycol monolaurate and polyethylene glycol laurate, such as diethylene glycol monolaurate and triethylene glycol monolaurate are also expected Is effective. Some polymers are also expected to have a toxin reducing effect, for example, poly Triol (molecular weight 200)-laurate, polyethylene glycol (molecular home 400)-lauric acid, polyethylene glycol (molecular weight 1000)-laurate, and polypropylene glycol laurate such as polypropylene alcohol monolaurate Ester. Among the ingredients of the present invention, it is believed to be other -11 effective to TSST-1 toxins.-This paper uses the Chinese Standards (CNS) A4 specifications (210x297 public waste) ....... ...... ^ ......... ^ ..... 玎: · · line ·. Ί (please read the precautions on the back before filling this page) 〇u Λ 6 Β6 Ministry of Economic Affairs Printed by the Central Bureau of Standards' Staff Cooperative V. Description of the invention (10) The compounds are i-glyceryl monocaprylate, glyceryl monocaprate, glyceryl monocaprylate and a mixture of glyceryl monocaprate, glycerin Based on myristic acid, glyceryl monopalmitate, glyceryl monostearate and glyceryl monooleate. According to the present invention, the non-absorbable ingredients of the present invention are included in the product exposed to golden yellow grapes Cocci can effectively inhibit the above-mentioned esters of toxic shock syndrome toxin 1 (TSST-1). For example, the effective amount has been found to be about 0.1¾ and higher, Preferably, according to the weight of the prepared solution, at least about 0.5S: weight / weight ((W / W) specified monoester or diester compound (or a mixture thereof). Preferably, the glyceryl group of the compound of the present invention The monolaurate / glyceryl dilaurate mixture contains at least 90¾ glyceryl monolaurate; more preferably it contains at least 95¾ glyceryl monolaurate. Preferably, the compound mixture should actually consist of glyceryl monolaurate. Composed of laurate. The effective component of the ingredients of the present invention can be a variety of different formulations, such as those currently used in commercially available lavage fluid formulations or higher viscosity lavage fluids. For example, the effective component of the ingredients of the present invention may be formulated with a surfactant, preferably a non-ionic surfactant, such as Cremophos RH60, Tween 20 or the like. The ingredients of the present invention may also contain preservatives such as methyl paraben or propyl paraben or the like. Compounds that produce greater viscosity, such as propylene glycol, can also be added to the ingredients of the present invention. Usually it is -12 with higher viscosity-This paper standard is common to China National Standards (CNS) A4 specifications (210x297) (Please read the precautions on the back before filling out this page) Installation · Line · Ministry of Economic Affairs Printed by the Bureau of Standards' Staff Cooperative Society 〇5〇〇 <:; _ nt_

Fifth, the description of the invention (ID part is better, and it will produce a formula that will stay in the vagina for a long time after application. A sample formula is as follows: 0.30S! W / W glyceryl monolaurate, 0.50¾ W / W Cremophos RH60, 2.00¾ W / W Tween 20, 0.30¾ W / W methyl paraben, 0..10¾ W / W propyl paraben, 1.0¾ W / W propylene glycol, 0.04¾ W / W FD & C Blue «Π (A kind of dye) and 95.76¾ W / W deionized water. Another sample formula contains 0.5 (UW / W glyceryl monolaurate, 1.50¾ W / W Cremophos RH60, 1.00¾ W / W Tween20, 0.30 ¾ W / W methyl paraben, 0.10 ¾ W / W propyl paraben, 0.043! W / W PD & C Blue (a kind of dye) and 96.56 ¾ W / W deionized water. Proposed on April 27, 1998 Pending U.S. Patent Application No. 343,965 and No. 316,742 filed on April 27, 1990 (both of which are incorporated herein by reference) have stated that glyceryl monolaurate is a kind of poison that can be used to suppress toxic shock 1 The effective ingredients of these two applications indicate that glyceryl monolaurate is a material that reduces the formation of TSST-1 toxin when exposed to Staphylococcus aureus in absorbent products The active compounds in the ingredients of the present invention can also effectively suppress the production of other types of staphylococcal toxins, especially «staphylococcal enterotoxins A, B, C, and TSST-1 with A. The active compound is placed in When it was on the absorbent material, it was found to be effective against the above-mentioned enterotoxins. The compound of the present invention effectively prevents the formation of Staphylococcus A, B, C and TSST-1 with A, proposed on October 30, 1990 US Patent Application No. 07 / 605,910 (Attorney Case-13-AA Family Standard (CNS) A4 Specification (210x297 Male Dragon) in this paper standard for easy use (please read the precautions on the back before writing this page) -Line · Ministry of Economic Affairs + 揲 揲 准 局 贝 工 消 # Printed in the cooperative society V. Invention Instructions (12) No. PPC369). The glyceryl monolaurate has also been found to be effective in inhibiting the production of pyrococcus Streptococcal Pyrogenic Exotoxins (SPE) A, B, and C, and A, B, F, and G are hemolysins produced by Streptococcus. Glyceryl monolaurate analogs are believed to be effective in inhibiting these Toxin production. Glyceryl monolaurate is used in solution and absorption Effectively inhibit streptococcal toxin production when the product is presented in the May 3, 1991 pending US patent application Ser. No. (Attorney Docket No. PPC 389) have illustration. The following examples illustrate the effects of the components of the present invention on TSST-1. Of course, these examples only illustrate the products of the present invention and do not limit the scope of the present invention. Λ 6 13 6 (Please read the precautions on the back before filling out this page) 装 · -14-This paper uses the National Minority National Standards (CNS) A 4 specifications (210x297 degrees). Printed by consumer cooperatives Λ6 ___ Π_6__ 1 · 5 5. Description of the invention () wm 1 Add glyceryl monolaurate to a commercially available lavage fluid, and heat these mixtures to dissolve the glyceryl monolaurate. The composition of the lavage fluid is composed of glyceryl monolaurate at concentrations of 0.1, 1.0 and 10.ox by weight of the lavage fluid. Specifically, in this example, Massengi 11 vinegar and water lavage containing only vinegar and water are mixed with glyceryl monolaurate. The solution in this example includes only Massengill vinegar and water irrigating solution, which is a commercially available product produced by Beecham Products, adiv 丨 s η of Beecnam Inc. in Pittsburgh, Pennsylvania, and three solutions Massengill vinegar and water lavage fluid have 0.U glyceryl monolaurate by weight, MassengiH vinegar and water lavage fluid have 1.0¾ glyceryl monolaurate by weight, and Massengi 1 1 vinegar and water lavage The liquid has 10X glyceryl monolaurate by weight. We have used the cotton tampon method reported by Reiser et al. In the Journal of Clinical Biology, Volume 25, August 1987, pages 1450-1452 to test the effect of glyceryl monolaurate. The method has been adjusted to allow the use of this procedure without the need for tampons or absorbent materials as described below. In our experiment, we used the freeze-dried Staphylococcus aureus species FR 1-1169 obtained by Dr. Mer 1 in Bergdol 1 of the Food Research Institute, University of Wisconsin, Madison, Wisconsin, USA. We mixed one milligram of freeze-dried Staphylococcus aureus species into one milliliter of brain heart infusion (Brai η Heart Infusion, referred to as ΒΗΙ) broth (from Deutsch, Michigan, USA-this paper is used in Η countries Standard (CNS) A4 specifications (210 > &297;) (Please read the precautions on the back before filling in this page) Outfit · Printed by the Ministry of Economic Affairs, Bureau of Standards, A Industrial and Consumer Cooperatives 5. Description of invention (14 ) Obtained from Difco Laboratories in Lishi), transfer the above mixture to a test tube containing five milliliters of BHI broth, mix thoroughly again, and incubate at 37 ° C for 24 hours before use to prepare a golden yellow Staphylococcus suspension. Afterwards, add the biological and culture base to the sterile centrifuge tube, spin in a refrigerated centrifuge at 2,000 rpm for nine minutes, and pour out the supernatant to make Jiu re-floating in phosphoric acid. Washed salt water ((PH4.0). This process is repeated five times for 10 minutes of washing. Ph4.0 solution is inoculated without transferring nutrients, and prevents bacteria from being exposed to vine salt when exposed to PH4.0 solution. Massengil 丨 and Summer's Eve lavage products performed this procedure as described above. Place one hundred milliliters of BHI fat (also obtained from Difco Laboratories) into each of ten 3.8 cm x 20 cm culture tubes. Reiser et al. Cellulose dialysis bags were prepared and sterilized in the reported manner. The dialysis-free dialysis bag (limited to a molecular weight of 8.000) was sufficient to provide 5.20 X 10 cfu / ml of Staphylococcus aureus at the beginning of the test. The golden yellow "staphylococcus suspension is inoculated. The test solution is prepared by adding solid glyceryl monolaurate to a commercially available lavage solution (Massengill and summer's Eve) and heating to 60 degrees Celsius. Lavage The content of glyceryl monolaurate in the liquid is 0.1, 1.0, and 1.¾ by weight. These solutions are cooled while stirring, and then added to contain 1.0 g Λ 6 13 6 by weight (please first Read the precautions on the back and then fill out this page) Binding-Binding · Thread. The size of this paper uses Chinese National Standard (CNS) A 4 specifications (210x297 gong) Λ 6 Β6 V. Description of invention (M Staphylococcus aureus has only Inoculation of dialysis bag. Separately add one gram of non-collateral distilled water to the dialysis bag inoculated with Staphylococcus aureus without adding any additional additives, this is called a control inoculum. Then each dialysis bag is inserted into the In the culture tube, and allow it to harden. I used two control inoculums, each of which was a serving. Therefore, in this experiment, ten culture tubes were used, and four contained control materials (two with Massengill vinegar and water lavage fluid). , Two pieces without), and six cultured meal tubes containing glyceryl monolaurate added to a commercially available lavage solution, the concentration of which is 0.1 mass to produce glyceryl monolaurate W / W Massengill lavage solution , With 1.0¾ glyceryl monolaurate Massengill lavage fluid with ester tf / W, and Massengill lavage fluid with 10¾ glyceryl monolaurate W / W. After a 24-hour incubation period, determine the cell concentration and TSST-1 degree of the culture tube. This sample The results are shown in Table 1. (Please read the precautions on the back before filling in this page) Outfit-Printed by the Ministry of Economic Affairs + Central Standards Bureau Employee Consumer Cooperative Co., Ltd. 17 This paper standard uses the Chinese National Standard (CNS) A4 specifications (210x297 public waste) 5. Description of the invention (16 Table Λ 6 1) 6 The effect of glyceryl monolaurate added to Massengill lavage on the formation of TSST-1 and the growth of Staphylococcus aureus ?) # After / i The color of the most FU yellow C gold bacteria 10 X / C

Sphericality increase vine concentration _ color most C yellow S gold SLO TSST-1 final volume h (milligram) only inoculum 91.20 9.96 6,309.80 Masseno i I 1 lavage 0.16 7.21 6,512.52 Masseno i Π lavage ( 0. IS: glyceryl monolaurate) 2.25 8.35 2,161.60 Masseno Π 1 lavage fluid (1.0¾: glyceryl monolaurate) 0.71 6.85 31.80 Masseno Π 1 lavage fluid (10¾ glyceryl monolaurate) 0.22 7.35 35.78 (please read the precautions on the back before filling in this page). Pack. Printed by the Ministry of Economic Affairs, Bureau of Economic Development, Beigong Consumer Cooperatives, a. The number of live Staphylococcus aureus cells indicated by the logarithm of 10. b Reiser et al. withdrew biology in application and environment, determined in December 1982, 1349-13 page 55 by the ELISA method. c Average determination of samples. -18-This paper is used in a national standard (CNS) Grade 4 (210x297 gong) ii ούύ ο 66 ΛΒ Printed by the β-Consumer Cooperative of the Bureau of Standards, Ministry of Economic Affairs 1 7 V. Description of invention () Table 1 The data shown in the figure demonstrates that at a concentration of 0. UW / W glyceryl monolaurate, TSST-1 decreases by 66¾, while at 1.0¾ W / W glyceryl monolaurate, a reduction of 95¾ is seen. At 10S; W / W glyceryl monolaurate concentration, the occurrence of TSST-1 decreased by 99¾. When the cells were exposed to Massengill lavage fluid containing 0.1¾ glyceryl monolaurate, the viable cell count was increased from 7.21 to 8.35 in the logarithmic concentration in the commercially available formulation of the control group only. In contrast, Massengi 1 1 lavage with UW / W glyceryl monolaurate produced a logarithmic concentration of 6.85 (reduced by 4.9¾), while Massengill lavage with 10¾ W / W glyceryl monolaurate The logarithmic regeneration concentration increased from 7.21 to 7.35 seen in the control group. The method used does not prevent the active ingredient from moving beyond the vortex dosing bag. But even from this point of view of movement, it still works. Example 2 I have also evaluated the growth of Staphylococcus aureus and the production of TSST-1 caused by the presence of Summer ^ Eve lavage fluid with and without glyceryl monolaurate. Summer ’s Eve lavage fluid is manufactured by C.B. Fleet Co. Inc. of Lynchburg, Virginia, and is available in the United States. Several lavage fluid products are sold under the trade name Summer ’s Eve, but the products tested in this example contain purified water, vinegar, and benzoic acid. The solution containing the SumneriS Eve lavage fluid and the unconcentrated glyceryl monolaurate was heated as described in Example 1 to dissolve the active ingredient. In this example, the content is 0.1, 1.0 and -19-this paper scale is used in China National Standard (CNS) A4 specifications (210 father 297 public goods) (please read the precautions on the back before writing this page) Binding · Order * Λ 6 Β6 V. Description of the invention (13 10 »; W / W glyceryl monolaurate concentration solution. Prepared as described in Example 1 and added with liquid solution

Epiester is used for acid report. Gui Zhi reports moon growth, fruit growth, and growth of bacteria. 2 ## Amount of liquid color Evaluation table Wash yellow to fill with gold to give e and amount EV to form a sample sphericity l vine concentration Yao I 8 ® # back / 1 color FU yellow ε gold bacteria 10 X / IV

Sphericality increase vine concentration _ the last color of the first color of the golden yellow gold bacterium LO TSST-1 the final amount (milligrams) only inoculum 91.20 9.96 6,309.80 Summer Ts Eva lavage 0.021 6.34 670.94 Summer Ts Eva lavage Liquid (〇. U glyceryl monolaurate) 0.005 5.74 27.87 Masseno Π 1 lavage fluid (1.03: glyceryl monolaurate) 0.006 5.83 4.60 Summex ^ s Eva lavage fluid (10 ¾ glyceryl January 0.12 7.08 4.00 cinnamate) a The number of live Staphylococcus aureus cells shown in logarithm to the base 10. b Reiser et al. Applied and Environmental Microbiology, December 1982, determined by the ELIS A method reported on pages 1349-1355. c Average determination of duplicate samples. (Please read the precautions on the back and then fill out this page) The Ministry of Economic Affairs + Central Bureau of Industry and Commerce A Industrial and Consumer Cooperatives printed this paper. The Chinese Standard (CNS) Grade 4 specifications (210x297 mm) are used -20-_136_ _136_ A6 printed by the Beigong Consumer Cooperative of the Central Bureau of Economic Affairs of the Ministry of Economic Affairs 5. Description of the invention (19) The results listed in Table 2 show that the viable cell count was significantly reduced from a log concentration of 9.96 to 6.34 when the commercially available lavage fluid was present (Reduced 3650, TSST-1 formation was also reduced from 6,309.8 0 to 670.94 (reduced 89¾). The reduction in the concentration of living cells, in addition to the acetic acid in the commercially available formula, is most likely the presence of benzoic acid (a known bactericidal agent) The data listed in Table 2 shows that, compared with the control group containing only Summer's Eve lavage fluid, TSST-1 formed at a concentration of 0. U glyceryl monolaurate reduced the level of at least 96S !, In the presence of 1.0 and 10¾ glyceryl monolaurate W / W, a reduction of 99μ! Compared with the control group, the presence of 0.UW / W glyceryl monolaurate produces a logarithmic concentration of 5.74 (decrease 9¾) live cells. There is 1.0¾ W / W glyceryl monolaurate, the logarithmic concentration is 5.83 (minus 8S;). For comparison, the 10¾ W / V glyceryl monolaurate solution in the Summers Eve lavage solution produced a logarithmic concentration of 7.08 (increased by 11JO. Example 3 In this example, we had a concentration of 0.1 in distilled water , 1.0 and 1 (U's glyceryl monolaurate suspension was used to evaluate the growth of Staphylococcus aureus and the resulting TSST-1 production. The solution was the same as in Example 1 except that no commercial products were used in the test. The above is evaluated by the quantity of 1.0 g. The results are shown in Table 3. -21-This paper scale is used in the national standard (CNS) A 4 specifications (210x297 public release) (please read the precautions on the back (Fill in this page again) Pack-Line-Λ 6 136 V. Description of the invention (20) Table 3 Effect of glyceryl monolaurate suspension in water on TSST-1 formation and S. aureus growth sample S. aureus gold The final concentration of the staphylococcus aureus TSST-1 bacteria The final amount of the final concentration of bacteria 1 ^ (xlO CFU / ml) (Log CFφ½ liter) (milligrams) inoculation water 91.20 9.96 6,309.80 (control group) oleic acid water in the base ester Gan Gui in X November (Please read the precautions on the back and then fill out this page) The oleic acid water laurel in the base ester is installed on the month 0¾-Ordered by the Ministry of Economic Affairs + Central Standards Bureau Staff Consumer Cooperatives to print a living gold shown in logarithm to the base 10 Number of yellow staphylococcus cells. B Reiser et al., Determined by ELIS A method reported in December 1982, pages 1349-1355. c Average determination of duplicate samples. -22-This paper uses the Chinese National Standard (CNS) A4 specifications (210x297 public goods) printed by the Ministry of Economic Affairs, Central Bureau of Economic and Social Development, Beigong Consumer Cooperative Printed Γ〇'ύύ ^ (:: V. Invention Description (21) Table The data presented in 3 show that when the concentration of glyceryl monolaurate suspended in water is 0. IX tf / W glyceryl monolaurate and above, TSST-1 formation is significantly reduced (99.9V) In addition, at a concentration of 0.UW / W glyceryl monolaurate, the toxin formation is reduced by a factor of 1,000 and there is a corresponding reduction of 99.6¾ in the number of viable cells of golden yellow grape bulbs. In increasing glyceryl monolaurate At the concentration, the anti-biological effect is mainly at 1.0¾ level. At 10¾ W / W glyceryl monolaurate, the anti-microbial effect is reduced, which may be due to the insolubility of the tested compounds. TSST-1 The reduction of the formation from 0.S: to 10¾ glyceryl monolaurate did not change significantly, and the number of viable cells showed significant changes. Yin Li 4 I once had non-absorbable contraceptive products, Today Sponge, with And without glyceryl monolaurate to carry out this example to evaluate golden yellow grapes @ 之 Growth and the resulting TSST-1. Today Sponge is manufactured by Kobu® Whitehall Laboratories Inc. According to its description, today Sponge contains Nonoxyno 丨 -9 and benzoic acid in a polyurethane foam sponge , Sodium dihydrogen citrate, sodium metabisulfite, sorbic acid and water. The average weight of the sponge is 7.0 grams. The glyceryl monolaurate solution in the water is heated until the glyceryl monolaurate is dissolved and then formed Pill to 1.0g of today Sponge to produce a weight of 0.11, 1.0 and lOM / W glyceryl monolaurate according to Today Sponge. 1.0g sample is inserted •-23-Λ 6 Β6 (please first Read the precautions on the back and then fill out this page)--Installation- Order _ This paper scale is easy to use Chinese sleepy standard (CNS) A 4 specifications (210 > < 297mm) Fifth, the invention description (22) Λ 6η 6 The inoculated dialysis bag contains TSST-1 producing Staphylococcus aureus with a concentration of 5.20 χ 10 cfu / ml at the beginning. The test results are reported in Table 4. Table 4 Glyceryl monolaurate suspension in water Effects of TS2 on the formation of TSST-1 and the growth of Staphylococcus aureus Sphericality Ϊ color the most FU yellow C golden bacteria 10 X / IV this golden yellow staphylococcus TSST-1 the final concentration of the final amount Λ 17 (Log 0? _L) (nanograms)

Today Sponge (control group) 5.66 8.75 12,635.21 Today Sponge (0.1 glyceryl monolaurate) 4.60 8.66 2,185.88 Today Sponge (1. (U glyceryl monolaurate) 4.46 8.65 799.99 Today Sponge (1.0¾ glyceryl mono Laurate) 0.13 7.13 3.29 a The number of live Staphylococcus aureus cells is shown as a logarithm of 10. (Please read the precautions on the back before filling in this page)-Installation--Line-Ministry of Economic Affairs Bureau of Standards Printed by A Gong Xiao # Cooperative Society b Reiser et al. Applied and Environmental Microbiology, December 1982, determined by the ELISA method reported on pages 1349-1355. C Average determination of duplicate samples. Printed by the National Standard (CKS) Grade 4 (210x297 public wastes), printed by the Quongqiang Bureau of the Ministry of Economic Affairs, A Gongxiaot Cooperative Society Λ 6 13 6 V. Description of the Invention 2 (3) The results reported in Table 4 are shown in 0.1¾ W / W glyceryl monolaurate, TSST-1 formed a reduction level of at least 82¾, and in the presence of 1.0 and 10¾ W / W glyceryl monolaurate, there was a reduction of 93¾ and 99.9%, respectively. Regarding cell viability , Compared with the control group, there is 0.1¾ W / W glyceryl monolaurel The acid ester produces a logarithmic concentration of δ. 66 (reduction of 1.0¾). In the presence of 1.0¾ W / W glyceryl monolaurate, the logarithmic concentration is 8.65 (minus 1.U), at 10¾ V / W glyceryl monolaurate At that time, it has a high resistance to biological penetration, resulting in a reduction of 18¾ living cells. Fen Case 5 Because commercially available lavage fluid is often abused as a contraceptive, I have experimented with glyceryl monolaurate to evaluate the spermicidal effect. We used the Sander-Craner test (using human semen) to evaluate the spermicidal effect of glyceryl monolaurate. The compound was prepared at a concentration of 100 mg / ml in saline. One ml of this solution was mixed with 0.2 ml Human semen is mixed quickly. Prepare a hanging drop of this mixture, and check the sperm motility within 20 seconds from the start of mixing. The highest application concentration, sperm motility is maintained for more than three minutes, indicating the lack of sperm activity These examples demonstrate that glyceryl monolaurate is a potent toxin inhibitor for the TSST-1 toxin produced by Staphylococcus aureus. The compound is a commercially available ingredient of the present invention. The concentration used does not actually inhibit -25-The size of this paper uses the Chinese National Standard (CNS) A4 specifications (210x297 g; li :) (Please read the precautions on the back before writing this page)

Λ 6 η 6 V. Description of the invention (Cell growth. Moreover, glyceryl monolaurate does not inhibit sperm motility. Example 6 In this experiment conducted by Dr. Patrick Schlievert of the University of Minnesota, glyceryl monolaurate had Add to 50 milliliters with different pan degrees........------------------------------------------------------------------------------------------------Be s ., _____________________________________-〆 ml of genus A streptococcus species C203, or a known type of Staphylococcus aureus MN8 that produces TSST-1. Samples containing genus A streptococcus C203 in the presence of 7% C02 will be at 37 degrees Celsius. Cultivation 1 2 Hours without shaking to reduce the exposure of the organism to oxygen. Except for shaking (at a rate of 200 revolutions per minute) and in a standard incubator, samples containing MN8 were similarly inoculated. The summary of the results of this experiment is shown in Table 5. ( Please read the precautions on the back before writing this page) t Dingxiang Printed this paper by the Beigong Consumer Cooperative of the Central Standards Bureau of the Ministry of Economic Affairs. The paper scales are based on China ’s Standards (CNS) Grade 4 (210X297). Λ 6 η 6 5. Description of invention g6) Table 5 The effect of glyceryl monolaurate on the production of toxins and cell viability of agaricus and aureus S5MN8 glyceryl 1 SPE type laurate CPU AB TSST-1 rubbing mg 800 ____;- ° 3 C20 ο ^ Ι ^ Ό ^ Ο25 · 50 · 75 · 0 · 00 · °-° -aaaL111251 0808080808080706 11111111 xxxxxxxx 1 5 2 3 2 Λ ο, 3 · 3 · 3 · 3 · 3 · 24300000 SS ^ NDNX > .NDNJ) .NX > .NX) .NO.N1) .NX >. 33.00.75 Mengpin Zhe: ^ (Please read the precautions on the back before filling in this page) Binding-Set MN8 Ministry of Economics Standard Printed by Bureau Ordnance Consumer Cooperatives 0 8.4 X 10y 0.05 9.0 x 109 0.1 9.4 χ 109 0.25 6.2 χ 109 0.5 1.8 χ ΙΟ10 .75 9.7 χ ΙΟ9 1.0 2.1 χ ΙΟ10 1.25 7.0 χ ΙΟ9 1.5 1.4 χ ΙΟ10 1.75 1 · 1 χ ΙΟ10 2.0 4.0 χ ΙΟ5 2.25 2.0 χ ΙΟ3 2.5 2.3 χ ΙΟ4 5.0 2.1 χ ΙΟ4 10.0 2.9 χ ΙΟ4 48 48 48 12 NX >. NDNDNDND NJD. NDNDNDNDND line. -27-This paper scale uses the Chinese National Standard (CNS) A4 specifications (210x297 g; Ιέ) Printed by β Gongxiaot Cooperative of Central Bureau of Standards, Ministry of Economic Affairs Λ 6 13 6 V. Description of the invention (P) Example 7 In this example conducted by Dr. Patrick sehlievert, glyceryl monolauric acid was given to the species of Rhizococcus spp. Which represents SPEA, SPEB or SPEC, and the species of R. sphaericus B, F and G, respectively The effect of esters on exotoxin production is evaluated. The method described in Example 6 was used to expose the organism to different concentrations of glyceryl monolaurate in the brain heart infusion broth. The users used were SPE A producing strain 594, SPEB producing strain 86-858 and SPEC toxin producing strain T18P. The toxin production was measured by Western immunosmear for 96 hours. This experiment determined the effect of glyceryl monolaurate on the production of SPEA, SPEB and SPEC toxins. The results are shown in Table 6 in Table 7F. Staphylococcus aureus species MH8 was also exposed to glyceryl monolaurate. The amount of TSST-1 produced by Staphylococcus aureus MN8 has been measured. The results of this test are shown in Table 7. Also produced by strains 594, 86-858 and T19P, sclerolysin 0 and S and B belong to streptococcal hemolysin, F belongs to staphylococci hemolysin and G 羼 Streptococcus hemolysin, once used 0.U sheep The dissolution of erythrocytes and 0.01 4S! 2-temporal ethanol as a reducing agent in 0.75¾ agar agar (4.5 ml / slide) in phosphorus buffer solution (PBS) was determined. PBS consists of 0.005 moles of sodium phosphate and 0.15 moles of HaCl (pH 7.0). The hemolysis phenomenon caused by adding 20 ul of cell-free culture to the recessed portion on the carrier 24 hours later was used to measure hemolysin production. In addition to the use of 0, 1S: -28-This paper scale uses the Chinese National Standard (CNS) A 4 specifications (210x297 public) (please read the precautions on the back before writing this page) f install < Λ 6 13 6 V. Description of the invention (27) Except for the removal of tributyrate as a standard, lipase is fixed in the same way as hemolysin. Table 6 also shows the results of the reduction of streptozotocin 0 and S. The results of these experiments demonstrate the effect of glyceryl monolaurate on the production of toxins caused by B. F and G in Tables 8, 9 and 10 respectively. The data shows that in the presence of glyceryl monolaurate, the amount of toxin and / or hemolysin produced by A, B, F and G anticocci is significantly reduced. (Please read the precautions on the back before filling in this page) f Printed by the Central Bureau of Standards of the Ministry of Economic Affairs, printed by the Industrial and Consumer Cooperatives-29 This paper standard is the National Standard (CNS) A 4 specifications (210x297) ¢ 6 13 6 V. Description of the invention ί8) Table 6 The effect of glyceryl monolaurate on Streptococcus spp. Bacteria. Glyceryl-laurate logarithm CFU / = #less (milligram / ml) ml (pencil / Ml) 594 (SPEA) 0 8.6 32 7.0 25 8J 03 4.0 10.0 S3 03 0.0 20.0 6.0 0.0 0.0 86-858 (SP £ B) 0 8.0 0.8 4.0 2.5 7.7 0 · 0 2.0 10.0 7.7 0.0 0.0 20.0 5.8 0.0 0.0 T18P (SPEC) 0 7.9 0.4 8.0 2J 7.9 0.0 8.0 10.0 6.1 0.0 0.0 a The inoculum size is between 10 and 10 CFU / ml. b Including the measurement of the dissolved diameter 0 and S in the measured diameter in millimeters. (Please read the precautions on the back before filling in this page) Printed by β Gongxiaot Cooperative Society, Bureau of Standards, Ministry of Economic Affairs -30-This paper scale uses the Chinese National Standard (CNS) Grade 4 specifications (210x297 degrees). Description of the invention (Qiao) 66 Λβ Printed by the Ministry of Economy + Central Bureau of Standards and Staff Employee Consumer Cooperatives 昍 fgooNH I Hanlu © «but Fu Fu sleep avoid stirrup buried call 1 葚 葚 ill " L 卜卜 9 9 2« 〇X oo Seven Ώ 2 jn tn 〇ri 〇n O mo H?? 00 〇m 3: ·-»r > o -ζ ^« 4 jo 2 □ o cs or * »〇oos. H $ Q o 葩 τ 世 涔 3 : Forgive horse | w JQ «s 2 ooo« 0 n 〇Ov H «sooom X σ \ ooo · 00 • J o > 〇cs mmm s < n« η > d «0 T- oooo ft s: oooo CN tN «N | mwm 5 • 0 tn 00 • S β, 1 fc Jro -ppr mi-< two f wi 〇8 8 § ίπ ας ^ # 31-. -1 £ ϊ / ωοΙ / ο · ιψ4 < ^ ® ^ ro (Song W / 轵 耘 w) I-1SS1 · 1 Ρ (please read the precautions on the back before writing this page) Wear · 鳑 ·, this paper The standard uses Chinese National Standards (CNS) Grade 4 specifications (210x297 g; 1J :) £: Λ 6 Π 6 V. Description of invention (30) Table δ Glycerol Glycerol a certain month on the role of R «chain enamel glycerin Monthly lauric acid (nanograms / ml) m 8 hours of interpersonal teaching cell / set and 24 hours of hemolysin cells / ml hemolysin_ 0 8.7 2 8.5 2 2. 5 8.1 0 8.4 0 10.0 < 4.0 0 < 3.0 0 Inoculum size 2. 0 X 105 / ml (please read the precautions on the back before writing this page) Packing. Line-Printed by the Ministry of Economic Affairs, Bureau of Standards, and Seen at the Consumer Cooperative Society 32-This paper size Printed with China National Standards (CNS) A4 specifications (210x297 public goods) Λ 6 Β 6 Employee Consumer Cooperative of the Bureau of Standards, Ministry of Economic Affairs V. Invention description (3 |) The effect of enamel in the glycerin phase is monolauric acid (milligram / ml). M 8 min. 8 hours to reconcile the cell / set and dissolve rfn. 24 hours logarithmic cells / sp. Hemolysin 0 8.3 7 8.3 7 2.5 8.5 0 8.3 0 10.0 < 10 ^ 0 < 1 〇3 0 Inoculum size 2. 0 X 1〇5 / ml-33-(Please read the precautions on the back before filling this page) Use the Chinese National Standard (CNS) A 4 specifications (210x297 mm) Λ 6 13 6 recognition!?: Fifth, the invention description (32 ·) Table 10 _Glyceryl January-like acid cool pair (} 靥 _ 球 铺 之Role _ _ time most _ glycerin period one lauric acid 8 hours 24 hours (milligrams / ml) logarithmic fines / li and dissolved and valerian logarithmic fines / set and dissolved rfn 褤 10 10 v3 inoculum size 2. 0 X 1 (// ml (please read the precautions on the back before filling in this page). Packing. Thread. Printed by the Staff Consumer Cooperative of the Central Standards Bureau of the Ministry of Economic Affairs. -34-This paper standard uses Chinese National Standard (CNS) A4 Specifications (210x297 mm) __136_ Λ 6 V. Description of the invention (33) Example 8 In this experiment conducted by Dr. Patrick Schlievert, attempts were made to induce the key bacterium C203 and Staphylococcus aureus MN8 to contain glyceryl groups It is grown on the board of monolaurate. When 5 X 106 CFU is coated on the agar plate, the lowest glyceryl monolaurate of strain C203 is inhibited. The preparation concentration is 1 mg / 100 ml. The 2 mg / 100 ml plate will not grow. When coating 7 X 10y CFU, the minimum inhibitory concentration of glyceryl monolaurate of strain MN8 was 5 mg / 100 ml. The 7.5 mg / 100 ml plate will not grow. This experiment was conducted on average twice a week for six months. The data shows that when there is an inhibitory level of glyceryl monolaurate, no costly growth is possible. (Please read the precautions on the back and then fill out this page) Installation · -Line-Printed by the Employee Consumer Cooperative of the Central Bureau of Economics of the Ministry of Economic Affairs -35-This paper standard uses the Chinese National Standard CCNS) A 4 specifications (210x297 male dragon )

V. Description of Invention () Λ 6 Π 6 Patent Application No. 80 108379 ROC Patent Appln. Ho. 80 108379 Supplemental Examples in Chinese-Annex I (Supplemental Examples in Chinese-Enel. I (June 81, June 6 (Amended & Submitted on June (^ .1992) g Example 1 The above mentioned hemostatic ridges to be tested were used. Prepare hemostatic plugs each containing oi%, ι · 〇% and 10% (weight tb) of the above-mentioned Lauricidin mixture, Based on the weight of the untreated haemostatic plug. Apply this Lauricidin mixture to the haemostatic plug to be prepared according to the general procedure for preparing the haemostatic plug of the present invention as described above in this application. It will contain dagger 31-11 " The hemostatic plug in is split into two parts. Then, the hemostatic plug treated by La ^ iricidin is tested according to the hemostatic plug method. This method is reported by Reise "et al. in the Journal of Clinical Biology, Volume 25, 1987 August 2014, pp. 1450-1452, the contents of which are shown and are included in this article for reference. It will be obtained from the United States, i / iscons in, Madison, Vine y〇ns; n University, Food Research Institute, Merlin Be, Dr. Gdoll, presented Golden Yellow® in lyophilized form Staphylococcus aureus FRI-1169, used in this experiment. A suspension of Staphylococcus aureus was prepared by the following method. One milligram of freeze-dried Staphylococcus aureus® was mixed with 1 mg of brain Heart perfusion solution (BHI) medium (tablets from Difco Laboratories, Detroit. Michigan, USA), the mixture is transferred to a test tube containing five milligrams of B IU medium, and the bottom is mixed again. Before use. Incubate for 24 hours at 37Ό.

Place 100 mL of brain-heart perfusion solution (B 丨 1 I) fat (also available from D 丨 fc 〇 夜 骏 室 .'Detroit, Michigan, USA ~) into ten pieces of 3.8 cm X paper size for medium use. CNS Standard (210x297; I!) (Please read the notes on the back and write this page on the wall) Install & Line. The Ministry of Economic Affairs, Bureau of Standards, Alpha Engineering Cooperative Du Du ¾ 81. 4. 10,000 sheets (H) V. Description of the invention () Λ 6 η 6 20 cm in each test tube. It is made into Xieweisu capsule and sterilized by the method reported by Reiser et al. Inoculation of this non-German vesuvin capsule with the above-mentioned gold trade color Portuguese suspension is not sufficient to provide lx 10s CFU / ml in it at the beginning. Place each test hemostatic base treated with Lauricidi η containing golden grape spheres into a piece containing B agar: 'In a control group (inoculated sacs (where there is no maintenance tube. Hemostatic plug ( This means that the above culture tubes do not contain Laruicidin) BHI S lipids, four tubes contain unused hemostatic plugs), bacteria in sterile sterilized capsules, and then the culture test tubes. The two groups of controls are called inoculum In the control group ") _, place the hemostatic plug) in the second control group containing BHI Qi in the second control group. Split the two hemostatic plugs into the Xieweisu S before they are placed in Xieweisu S Therefore, in this test, the above-mentioned control Luo (two use hemostatic plugs and the other contains the above-mentioned Lauricidir for the test. Insert into each capsule, each of which contains propofol treated with fat. Use + support: Yong support has been processed (please read the precautions to be filled out by Sun before filling out this page) Binding · Order · Line-Ministry of Economic Affairs, Bingyangzhun /! 00: Industrial and Consumer Cooperation Du Printed I-Time Two FR Small c each @ 0 中-球 ί 1 Serve the table blood I® starts from stop color The yellow test is for the gold test, and for the test, for the degree, the venom of the trichosanthin is 24 m yokepei ^ Ttt-.p 7 骞 37 and yu 69 and 2 paper size in the middle of a B home Sample Bi (CNS) T4 specification (2 丨 0x297 male dragon) 81.4 ·] 0,000 sheets (H)

Jat 05 ^ 0 A 6η 6 V. Description of the invention () Table 1 The hemostatic stopper treated with 1 ^ 11 "丨 £:; £) 丨 11 For the TSST-1 produced by the golden yellow grape country and its growth The effect of the sample is golden "The initial concentration of Staphylococcus aureus TSST-l a). The final salience of Staphylococcus aureus the final slurredness of Golden Staphylococcus aureus® b) The final stagnation of TSST-1 Degree a) (CFU / ml) (Cochroch (CFD / ml) (/ ml) (mg / without tampon (10θ) 1.0 / ml) HD. (1θθ) • 100 10.0 ml) 50 (control ) Untreated 1.0 HD, .100 10.0 31 Hemostatic plug (control) Hemostatic plug 1.0 ND 794 10.9 15 w / 0.1% La ur icid Hemostatic plug in 1.0 HD 158 10.2 0.09 v / 1. ΟΧ Laur icid ί η Hemostatic plug 1.0 HD 316 10.5 0.08 (please read the precautions before filling in this page) Pack < Order · line. r |! η y) JL i. J ·, LI: i · 11 3. w /10.03: ·

Laur i c i d i η HD = not measured. Re ise "The detection limit of the EL ISA method by others is 0.5 milligrams Γg / ml. A) = Press Reise" Wait for the withdrawal of biology in the applied public environment, December 1982, pages 1349-1355 The ELISA method reported in the test. Its content is shown here and for reference. B) = Log value expressed at the bottom 10_. All the above data are the average measured value of the sample. The paper is used in the middle of the scale. National Standards (CNS) Ή specification (210x297 g; «:) 81. 4. 10,000» H) ^ 0550 ^ Λ (5 __ Β 6_ V. Description of invention () The data in Table 1 shows that the staphylococcus aureus, In the presence of a hemostatic plug containing o.ixu / w) Laurie i. &Quot; d in, pseudo-tb when exposed to a control hemostatic plug that does not contain LaYr ί ci (Π η., Produces a toxicity reduction of 51% Shock syndrome group toxin-1 (5 51'-1), regardless of the fact that the actual number of surviving Staphylococcus aureus cells does not decrease in the presence of Lauricin-treated tampon. This data is further Show golden

I. Staphylococcus aureus in the presence of a hemostatic plug containing 1.0X (w / w) and 10.0X (w / w) Lauricidin, with the same number of golden yellow® staphylococcus bacteria in a control hemostatic plug without Laurie 丨 din 'Compared under the existence, there will be a reduction

I 99% of TSST-1, once again irrespective of the fact that in the presence of a hemostatic plug containing Laruicidin, the number of viable Staphylococcus aureus cells did not decrease. This data shows that when aureus »Staphylococcus aureus cells are exposed to hemostatic plugs containing varying degrees of Lauricidin, although the number of surviving cells has not decreased. However, the TSST-1 produced by the cells is real (please read it first. Matters needing attention to write this page again) Outfit-line. Ministry of Economic Affairs 屮 央 梂 准 / (0CI: Cooperation between the industrial consumer and the Du Yin system and · Xiuxi at the drop-off point 0¾ The instinct is at the 相 π 1 Come to save _ di in order to exchange grapes often, CI will be there. ¾ Shengyuetong, 1} The color is produced by -1 au in the case of yellow cells S ^ ST L ί condition gold fine blood, TS 1 * except Qian ’s It ends at 0. The cause of the elimination is due to the existence of stagnation in the life above the birth. The leap is low in the quality of the blood. The leukard, such as S, the drop in the ball, or the chopsticks in the wine, the IRI is low. Zhongli is not real '1C. The color drop situation is confirmed. ^ Use yellow to express love inin its -lLa to make Jin Xian's 1 () 1 (1 but Su Xin within the letter is 1C1C u U The number of groups-the limit of the LaLa number is the middle of the number, but the m is due to the female crossing the age of 10 although the cell is female. 81. 4. 10,000 sheets (H) The size of the paper is used in the S family. Sample standard (CNS) f 4 gauge (2) 0x297 mm) Λ 6 Π 6 1. Description of the invention (low. In addition, in preliminary studies. Pseudo-use of the main withdrawal organisms present in the vagina (i.e., yoghurt defend a nl π t 0 ha P i 1 1 II s ar irioo hi 1 us)) It was found that when the withdrawal creature was connected with the example 1 hemostatic plug containing l.OXU / w) Lauricidin, there would be no adverse effects. G cases ?. A second experiment was conducted to evaluate the use of different amounts of Laruicidin t In the presence of the treated commercial hemostatic plug, the growth of Staphylococcus aureus cells and the TSST-1 produced by it. It was adopted in the experiment of this Example 2 and was purchased on the public market for TaBpaXt & menstrual bleeding Plug (regular size, product number 8L015Z). These tampon are manufactured by Tanbrands of Lake Success, New York, USA. Tanpax * tampon contains (please read the precautions before filling out this page) Ministry of Economic Affairs The standard printed by the Zhagong Consumer Cooperative Association is based on the use of photometry to return to the root. It is correct. Take.% «Materials and test strips, 10 weights, test the blood and remove the blood and stop the blood. Cut forward, cut off the pieces that have been made by D blood, and stop cutting. Regulations, fine dryness and s 'fine dryness in the order of the dry place back to 0'. At the place where the chao in Cheng Kai has a sutra. Ur report 1 blood two, stop at La., Stop the stopper 'ί 绖 绖 1 The layer 2 will make the blood ax C example 2 core, and stop the dry basis of the ΠΡ different dryness table before the test is based on the acceptance test Cheng.111. 膣 10 has already announced that the two IDs will be the same as that of the suction test. Scription Ur. ^ Ran blood knot La quasi-La. Stop the test The 5fc scale of the paper is used in the national standard (CNS) fM specification (210x297 g; «:) 81. 4. 10,000 sheets (H) Λ 6 Π 6 V. Description of invention () Table 2

The effect of Lauricidin-treated TAMPAX hemostatic plug (product < No. 8L025Z) on TSST-1 produced by Staphylococcus aureus (FRI-1169). Golden yellow grapes Golden yellow grapes

I ball spheres of a cell TSTS-1 of a cell the final concentration of the final sample of the final concentration b) the posterior degree a) (CFU / ml) (/ ml) (mg / ml) (108) 1 Γ. 7 2 27.50 10.30 3.70 10.40 1.05 .13 8.33 0.19 OXLauricidin 5,248 Hemostatic plug (control) O.IXLauricidin 199 On the hemostatic plug l.OXLauricidin 251 On the hemostatic ridge lO.OXLauricidin 2 Ministry of Economic Affairs, Bureau of Standards, Industrial and Commercial Cooperative Print Measured on the tampon, er et al.). a) = by the EL IS A method (R " e i s b) = expressed in terms of base 1 0 to 1 ε. All tests were performed after 24 hours of incubation at 37 ° C. All the above figures are the average measured values of two test warts. 81. 4.〗 0,000 sheets (Η) (please read the precautions first and then write this page) The standard of this paper is easy to use a Η 家 标 毕 (CNS) Ή specification (210Χ.25Π 公; ": ) Λ 6 _Π6_ V. Description of invention () (please read the back-end notes # 塡 write this page) The data in Table 2 shows the golden yellow «Grape SS, using different amounts of Lauricidin treated Tampax '* The presence of a brand hemostatic plug was compared with when it was violently transferred to the control hemostatic plug of Lanricidin. The pseudo-production of less TSST-1, the degree of reduction in toxin production is related to the amount of Lauricidin in the hemostatic plug. Tanpax * tampon treated with 0.1% fi% Lauricidin, compared with the control tb, caused

86% of I TSST-1 is reduced, and by 1% and 10% (weight ratio)

The Tanpax * ik thrombus treated by Laur 丨 cidin caused a 96% and 99% reduction respectively. The results of using the Tanpax * brand hemostatic plug also showed a decrease in the gold / yellow grape ball® cell count; this effect depends on the degree of Lauricidin's hemostatic plug. At the end of the 24-hour incubation period, the log concentration of golden staphylococcus aureus® cells in the presence of the control-hemostatic plug was 11.72;

In the presence of Lau "ic 丨 din's hemostatic plug, the number of seals is 10.30 (12% reduction); golden yellow staphylococcal cells contain 1.0%

Lau "丨 cidin's hemostatic plug has a logarithmic concentration of 10.40 (a reduction of 11%); and the golden yellow globulus S5 · cells contain! 〇% Lau" icidin's hemostatic plug, its logarithmic degree is 8.33 (Decrease by 29%). The Ministry of Economic Affairs has approved it and printed it by the Liaogong Xiaozhong Cooperative for the third session, to evaluate the use of L au "icidi η treated cities with different fees. Hemostatic presence , '&Quot; Golden color paste grape® cell growth and TSST-1 produced by it. In the test of Example 3, the P 丨 ayte X * brand menstrual hemostatic ridge (available on the open market) was used ( Regular size. -7-81. 4. 10,000 sheets (H) The size of this paper is easy to use China Sleepless Standard (CNS) 1M specification (210x297 mm) Λ 6 Π 6 V. Description of invention () Product number 3496P). These hemostatic plugs are made by Del aware, Dover, Playtex, Inc. of the United States. Playtex * hemostatic plugs are made of Quan Xuanzi Panwei, and have a retrieved fine but not covered with g. Debris. Before testing , Retrieve the hemostatic plug and cut off the hemostatic plug. According to the above general procedure, prepare two portions containing 0.1%, 1.0% and 10% Lau "icid The treated hemostatic plug in is based on the weight of the untreated hemostatic plug. Use a two-pile Playtex * hemostatic plug without any Lauricidin treatment (where the fine pill has been excised) as a control. Then follow the instructions in Example 1 The procedure and chopsticks, test the dried tampon L, a u'r icid ί η treated tampon and untreated control. The test results are reported in Table 3. (Please read the notes first Please fill in this page for details) ί Installation-Order · Line. The Ministry of Economic Affairs Central # quasi / ioa industrial and consumer cooperation Du Yin Μ 8 This paper standard is used in the middle of the trouble Η home standard Bi (CNS) Τ4 specifications (210X297 public) 81. 4.] 0,000 sheets (Η) V. Description of the invention (Λ 6 Η 6 Table 3 Lauricidin-treated PLAYTEX tampon (product number, 8L025Z) For TSST-1 produced by Golden Yellow® Grape Ball Room (FRI-1169) The effect of the sample is the final concentration of the golden yellow spheres® cells. The final concentration of TSST-1 in the golden yellow spheres® cells b) After the concentration a) (please read the precautions before filling in this book (Page) (CFU / ml) (/ ml) (1〇θ) OXLauricidin 3,3 8 8 1 1.-5 3 tampon ( Photographs) 0. l ^ Lauricidin 2 13 10.33 On the tampon lO ^ Lauricidin 131 10.12 (mg / ml) 10.86 .69 .38 The Ministry of Economic Affairs, Bureau of Standards, Work and Cooperation, Du Printing, i «1 揲 上The number of IS10 on Γ1 is 0au, and the EL bottom is filled with blood. The blood is stopped by the test. 0 stop = || there are 10 to 8) 6) the French side 1 is set to enter the time. A small value of 24 determines the average value of Pingjun Ping, etc. 37 搽 er is shown in the test. Is the list of parts Reia are both 9 copies of the paper standard xiao family standard (CNS) Ή specifications (210X297 public: ¢) | § test 81. 4. 10,000 sheets (H) ^ 055Cd Afi Λ ο __IV6_ V. Description of the invention () I ·, I The data reported in Table 3 shows that the 绖 is produced by Golden Grape Ball®! · -I The amount of TSST-1 produced, when using 0.1: C , 1.0 ¾ and 1 0. 0¾ La uricidi η. Playtex * hemostatic plug at ϋ, when compared with TSST-1 eggs produced in the presence of a control tampon without Lauricidin, the pseudo-individual reduction is 75% , 96% and 97%. On the other hand, compared with the control value of l: b, at the end of the '24 -hour incubation period, the Playtex * hemostatic plugs treated with 0.1%, 1.0% and 10.0% Lauricidin will become 10%, 12% and 26% less golden yellow grape spheres. Example 4 "A fourth value experiment was conducted to evaluate the growth of Golden Staphylococcus aureus® and TSST-1 produced by it in the presence of another brand of Lubricant Hemostatic Plug treated with different amounts of Lauricidin. In the experiment of Example 4, the Rely * brand tampon for menstruation (regular size, product number 2 060 LC01A) purchased on the open market before September 1980 was used. This (please read the precautions before filling out this page) Central Standards of the Ministry of Economic Affairs; FT Gong Xiao Zhi Cooperative Society Yin Yu Before the brewing-use the return trip The order taken by the hair salon * Ester dimension measurement. Its description er5g thanks to the general 1 η quasi-f ct in the ester. In an id base, ΓΟ scattered poly rope 1C is blood 1 P sub-cobalt fine upper urfi only examples i 丨 Lalni 犲 with spinning back contains at least lys nn from roots. 10 wild ^ root ci blood wrapped with ridges and blood after stopping the normal blood ¾ .: Zhi Liran e ί pseudo-stop 0 island. o.yl It has a separation of 1 η I hi things-its cut, location id according to 0 this element. Button% 1C to the country. Wei Zhongxi 1, Ur is a beautiful confession. It is based on La as a base, and the non-blood is made by the scorpion. Two unfinished pieces of nb, such as a single piece of Ga, will be processed into two fine papers of 5 good-size edges and used in a 漢 家 榣 毕 (CNS) T4 specification (210x297 g) 81. 4. 10,000 sheets (Η) Λ 6 Π 6 V. Description of the invention () Sequence and conditions, test the dried icidin-treated tampon and untreated control tampon. The test results are reported in Table 4. (Please First read the back and the matters needing attention # 塡 write this page) 装 · 线 < The Ministry of Economic Affairs, Pyongyang quasi-bureau, Migrant Workers and Consumers Cooperatives Co., Ltd. Yin Yu 11 1 81. 4. 10,000 sheets (H) This paper is used in S countries. Bi (CNS) TM specification (210x297 male dragon) V. Description of the invention (Λ 6 Π 6 Table 4 Lauricidin-treated RELY hemostatic plug (product number 2060LC01A) For the TSST generated by Staphylococcus aureus (FRI-1169) -1 effect sample. Golden yellow "5 The last degree of Staphylococcus aureus cells The last degree of TSST-1 of golden staphylococcus aureus cells b) After the degree of a) (Please Please pay attention to this item. Sun Guo wrote this page) (mg / ml) Central Ministry of Economic Affairs, Duxin (CFU / ml) (/ ml) _ (1〇8) OXLauricidin 12,303 12.09 Hemostatic plug (control Substance) 0.1S: Lauricidin 2.818 11.45 on the hemostatic plug l.OXLauricidin 1,995 11.30 1. on the hemostatic plug 10.03; Lauricidin 1,096 1 1.04 0. On the hemostatic ridge a) = measured by ELIS Λ method (R eiser et al.) Obtained. B) = Log value expressed as the bottom 10. All test kneading is performed after incubation for 24 hours at 37 °. All the above numbers are the average measured value of two test kneading. 6 4.32 6.92 54 09 Binding · Stranding · 12 Paper-scale, easy-to-use, trapped, and HF home standard (CNS) < f 4 specifications (2 丨 0X297; ":) 81. 4. 10,000 sheets' (H) A 6 116 V. Invention Explanation () The data reported in Table 4 shows the TSST-1 Μ produced by the golden yellow jg. In use 0.1%, 1.0% and 10.0% Lauri C ί di η Treated. In the presence of kely * hemostatic plug, when compared with the amount of TSST-1 produced in the presence of a control hemostatic plug without [auricidin, Pseudo-respectively decreased by 8 9%, 97% and 99%. However, at the end of the 24-hour incubation period, at the control hemostatic plug, the degree of the golden staphylococcus cell tapir (under the

I The logarithmic value of bottom 10) is assumed to be 12. 09, and in the presence of Rely * hemostatic plugs treated with 0.1%, Ι.οχ and 10% Lauricidin, the degree of staphylococcus aureus cells (called bottom 10 logarithmic values), which are 11.45 (low 5.3%), 11.20 (low 7.4%) and 11.04 (low 7.8%) 0 Fang Gong F5-conduct the fifth value experiment to evaluate the use of different amounts In the presence of commercially available hemostatic plugs treated with Lauricidin, the growth of S. aureus cells and the TSST-1 produced by them. In the experiment of this example 5, the o.b., brand menstrual tampon (available in regular size, product number 0 6 9 4 T) purchased on the public market was used. These hemostatic plugs are from the United States HJ, Milltown. (Please read the back of the precautions # fill in this page) to install · order _ line. The Ministry of Economic Affairs is accurate and the A Industrial Consumer Cooperatives printed 31 cotton in the former Sr versus. In the b bract ridge ridge 〇 Xuan Xian% of the blood of the cover cover 10 only reason containing S root and beyond the ridge. % 处 | 0 血 奋 岭 ο 绖 id only has blood 1 but 1C ί unstopped, with Ur bmf ^ ll% .La_ ο 渑 切 '' 0. 龍 何 13 This fine ffll has blood-. Back to the detailed content of the end of the distribution of ig copies, including the preparation of two sets of the company's package two ridges and cracks. The blood sequence makes 1¾ things stop 1η. 81. The general ID quasi-on is mixed with the previous ics rs. The test for Ur is a pe flower. La member's paper scale is used in the national standard (CNS) TM specification (210x297 mm). 4.1.4 0,000 sheets (Η ) Λ 6 Π 6 V. Description of the invention () Blood plug (where the detail "I has been excised) is used as a control. Then, according to the procedures and conditions described in Example 1, the dried Lauricidin-treated stopper and untreated control hemostatic plug were tested. The test results are not reported in Table 5. (Please read the back-end notes first # fill in this page) cf: by the Central Bureau of Standards of the Ministry of Economic Affairs: Printed by the Gongxiaozuo Cooperative

The size of the paper is based on the Chinese Standard for Sleepy Family (CHS) TM specifications (21ΠΧ297 公 ¢) 81. 4. 10,000 sheets (H) L055G4 Λ 6 Π 6 V. Description of the invention () Table 5 La * jricidin treated ob * hemostatic plug (Product- · No. 0 6 9 4 T) The most effective sample of TSST-1 for the effect of TSST-1 produced by the golden yellow spheroid window (FRIM169) The final concentration b) The final concentration a) (Please read the precautionary code and fill in this page first) (CFU / ml) (/ ml) (mg / ml) (1〇θ) OSiLaur ic id in 3 , 3 8 8 1 1, 53 Hemostatic plug (control) 0. l% Lauricidin 15 8 10.20 On the hemostatic plug l.OXLauricidin 316 10.50 On the hemostatic plug 13.46 3.26 0.28 Installation · Order_ line. U a The Hong Kong Bureau of Industry and Engineering ’s Consumer Cooperative ’s Yinhong ’s test draws the number of IS10 gEL samples described by the test. II II is available in a) b) the French 1C average test institute \ 1 / person etc. Γ e. After the advance, the hour value is small and the 24 test is under the same level. The sample V 3 test is shown in the copy. The Re values are both 2 0-5 11 -7. a National Standard (CNS) Ή specification (210x29 / male; ¢) 81. 4. 10,000 sheets (H) Λ 6 _ [V6_ V. Description of invention () The data presented in Table 5 shows that it contains 0.1%, 1.0 % And 10% (weight-fi ratio) the amount of TSST-1 produced by Staphylococcus aureus in the presence of ob * hemostatic plug of Lauricidin • When compared with the control ob * hemostatic plug without Lauricidin, the TSST- When comparing one quantity, the system reduces 75%, 98% and 98% respectively. In the presence of a control hemostatic plug, the golden yellow "concentration of the bovine cell (expressed at the bottom 10 to 10)) is 11.53. Used in 0.1%, 1.0% and 10% (weight ratio)

In the presence of Lauricidin-treated OB * hemostatic plugs, the concentration of golden yellow staphylococcus aureus® (expressed as a base value of 10%) is 10% (low 11%) and 10.50 (low 8. 9%) and 9.98 (low 13%). Palace Example 6 A sixth value experiment was conducted to evaluate the growth of the Staphylococcus aureus® cells and the TSST-1 produced therefrom in the presence of different amounts of Lauricidin-treated commercially available tampon. In the experiment of Example 6, the Kotex * Security * brand menstrual tampon used in the open market (regular size, product number 5C0907C) was used. These hemostatic plugs are sold by the United States, Visconsin, Neenah, KiubeKly-Clark. It contains (please read the back and attention matters # fill in this page) to install-order _ line · Ministry of Economic Affairs Bureau of Central Standards ^ work elimination cooperation cooperation du printing-the pre-order is not yet the test of the program. The general reason is to take blood at I. The Scriptures only use the usual overrides on Μ in_it to have a root and a base of Ur. .10 is to believe that the urfis are sold to Larrxi. _ 合 非 止 .. ¾ of te16 -1 is completed; J and the blood rupture ％ stop only the place where Xuan Luo fine α is returned. Iη 40 ene take 彡, place id and C of 1? Jing 1C flower poly plug ο not Ur cotton by blood with La% only, He 60 to put any ridges and-blood making this paper scale side in use CNS TM specifications (210x297); ":) 81. 4. 10,000 (H) 205 ⑽ Λ6 _Π6_ V. Description of invention (fine spots have been removed) as a control. Then, according to the procedures and conditions described in Example 1, the dried hemostatic plugs treated with Lauricidin and the untreated control tampon were tested. The test results are reported in Table 6. (Please read the back and the precautions # fill in this page first) Binding-Order ·-Line. The Ministry of Economic Affairs is accurate and the translator works to cooperate with Du Yin 3i. This paper is used in the standard a Η 家 槀 准 (CNS) A 4 Specifications (2 丨 0X297 public; «:) 81. 4. 10,000 sheets of '(Η) Λ 6 _____ Π_6_ V. Description of invention () Table 6

The effect of Lauricidin-treated KOTEX SECURITY hemostatic plug (product number 5 C 0 9 0 7 C) on TSST-1 produced by S. aureus (FRI-1169) golden yellow The final concentration of TSST-1 of the most samples of cocci cells b) The final concentration a) (CFU / ml) (/ ml) (mg / ml) (10θ) 11.-2 3 10.19 0% Lauricidin 1,698 Hemostatic plug (must take photos) 10.29 4.90 10.63 〇.〇9 10.63 0.05 0. 1% 1, auricidin 19 4 on the tampon l. OJ ^ Lauricidin 4 2 6 on the tampon, the Ministry of Economic Affairs, the central government, and 11 Gongxiao " Cooperative Du Printed by lO.OKLauricidin 426 on the hemostatic ridge a) = Guided by the ELISA method (Reeise "et al.). B) = Log value at the bottom 10 indicates no. The measurement is carried out after 24 hours of incubation under 37 ° C. All the above numbers are the average measurement value of two trials. -18-81. 4. 10,000 sheets (Η) (please read the notes first Please fill in this page again for details) This paper standard uses the Chinese Standard for the Poor Family (CNS) Ή specification (210x297 g; ":") 0 ^ l ~ Λ 6 _; _IV6_ V. Explicit description () The data presented in Table 6 shows the TSST-1 produced by the golden betaine weight β in the presence of Kotex * tampon containing 0.1%, 1.0% and 10% (weight ratio) Lauricidin When compared with the amount of TSST-1 produced under the same test conditions in the presence of the control Kotex * hemostatic plug without Lauricidin, Tong decreased by 52%, 99% and 99% respectively. Containing 0.1% and 1.0 % And 10% (weight ratio) the degree of staphylococcus aureus cells in the presence of the Kotex * t haemostatic plug of Lauricidin (expressed at the bottom 10 to 丨 0 g value), the pseudo-individual is 1 〇 · 2 9 ( Low 9 4%), 1 0 6 3 (low 60%) and 10.63 (low 60%) + '. As can be seen from the foregoing examples 1-6, there are many hemostatic plugs, one of which is split by the inventor ( Example 1), the other is commercially available (Examples 2, 3, 5 and 6) and one of them is commercially available but it is obtained by the municipal distribution office to obtain the standard of the Ministry of Economy and ^ Gongxiaozhong Cooperative Indies Back (Example 4). The above have been treated with the same level of Lauricidin treatment, the Laurie i din is a commercially available mixture. It contains 93% by weight of glyceryl monolaurate and 3.5% of eggs. Glyceryl laurate. The numbers reported in Tables 1-6 show that, depending on the content of Lauricidin in the hemostatic plug, when in the presence of a control hemostatic plug that does not contain Laurie · idin, under the same experimental conditions, the golden yellow The amount of lib produced by TSST -1 is relatively high, and the former golden yellow spherule produces false TSTS-1, or in other words, it is prepared without the production of TSST-1. Example 7 In this evening example 7, the button type hemostasis used in the example I was used. Based on the general procedure described above in this article. Preparation contains 0.1%, 0.5% and -19-81.4,] 0,000 sheets (Η) (please read the precautions before filling in this page) This The paper standard uses the Chinese Η home sample standard (CNS) T4 specification (210X297 male dragon) Λ 6 B 6 ^ 〇550i 5. Description of the invention () 1.0% L auricid ί η Hemostatic plug to be tested (untreated The amount of the hemostatic plug to be tested is the benchmark), and the test is performed according to the hemostatic plug method described in Example 1. However, in this Example 7, hemostatic cysts were inoculated with different types of Staphylococcus aureus before insertion of Lauricidin-treated hemostasis. The various Staphylococcus aureus species tested are indicated in the table. At the beginning of the experiment, the staphylococcus aureus had a thorax of 1 × 10Θ CFU / ml. The Staphylococcus aureus species FRI-1169 used in this Example 7 that produces TSST-1 is pseudo-derived from the United States, Wisconsin, Madison, WigoOnsin University, Food Research Institute, Dr. He "丨 ίπ Be" Sdoll. It is 1169W that produces TSST-1 golden "5 Staphylococcus species, pseudo-derived from Dr. Fred Quimby, V.-HD, Cornell K School, New York City, New York, USA. The third Golden Staphylococcus species (special It is a subgenus of FRI-1169). It is a single free mother species and is named TSS single ion. This TSS single ion can be obtained in a lyophilized state and obtained from the United States. Mew Jersey, Hill town, Luren Products Company, Dr. SK B "own-Sk" 〇bot. The fourth species will produce TSST-1 Staphylococcus aureus species, named Η η 8, from the United States, Minnesota, Hinneapol is-st. Paul, HI innesota University, Patrick Sch I ie "vert 丨!?". The fifth species will produce TSST -1, a gold member. It is named 1 1 8 7 and is fake from the United Kingdom, Leeds, Leeds University, Keith T. Holland 丨! 9 persons. All gold-colored balls tested in this example are exempt. Can be taken from the above The individual prepared the suspension of each of the stems and stems as described in Example 1 and used the Chinese Standard (CNS) T &I; i Regulations ΙΜ210Χ297 Gong; ¢) (Please read first Contrary to the precautions, please fill out this page) Install &Line; Line · Ministry of Economic Affairs, Bureau of Standards, FT, Industry and Consumer Cooperation Duin 5i 81. 4. 10,000 sheets (H) A 6 Π 6 L055G1 V. Description of invention () Blood plug Before, it was used to inoculate the bladder, and then tested according to the hemostatic bladder method described in Example 1. The hemostatic plug to be tested that did not contain any Lauricidin on both sides was used as a control. The test results are reported in Table 7. The results shown in Table 7 show that the generated TSST-1 pseudo-decrease with the increase of the 相 Lauric 丨 d η 犥 度. This pseudo-see is seen in all five strains tested. From the test results The conclusion is that the Lau "icidin effect found in Examples 1-6 is not specific for any specific | TSST-1 Staphylococcus aureus species. (Please read the notes beforehand #Item write this page) The Ministry of Economic Affairs, the central standard 汓 贝 工 消 " Cooperative Inno 1x 2 81. 4. 10,000 sheets Η) The 5fc scale of the paper is used in the S Η family standard (CNS) Τ4 regulations (210X297 public release) 5. Description of the invention (Λ 6 Π 6 Jing Lang 屮 'k \ $ X. η [\ · lv Ψ: soil f , Ά Table 7 L aruici .. ¾ Staphylococcus din treated hemostatic plug for the growth of various golden yellow species and the effect of TSST-1 produced by it produces TSST-1 LAUR IC ID IN produced by TSST-1 ® Species of a) 缌 最 b) (96) (withdrawal) 1 1 69 V None 56.54 (Quimby® species) 0. 1 17.54 0.5 0.35 1, 0 J 0.07 FR I-1 1 69 None 48.75 (B ergd 〇1 1 germination) 0.1 5.06 0.5 .5 0.04 1.0 '0.03 TSS single substance no 53.04 (FR .1-1 1 6 9 subspecies) 0.1 2.25 0.5 1.27 1.0 0.48 Η η 8 no 66.30 ( Schlievert (reserved species) 0. 1 0.66 0.5 0.12 1.0 0.05 1187 — None 46.80 (fl ο 丨 丨 and species) 0.1 6.63 0.5 0.92 1.0 0.58 22-This paper size is used in China (CNS) T4 specifications (2) 0X297 Public; ":) 81. 4. 10,000 sheets \ H) (Please read the notes before filling in this page) (Pack-Line · 205 504 Λ 6 Π 6 5. Description of the invention () a) = Based on the weight of the untreated tampon. b) = After 24 hours of incubation at 37¾, TSST-1 紾 fi 〇 in each tampon * * All samples were tested by ELISA (Reiser et al.), and the amount of TSST-1 produced was tested. Example 8 In Example 8 'of this study, a tampon containing various fatty acid esters was tested to determine its effect on the growth of Golden Yellow Grape Ball (FRI-1169) and the production of TSST-1 from it. In this Example 8, the type of tampon used in Example 1 was used. All the hemostatic plugs to be tested weighed 2.6 grams. Dissolve 0.65 g of various fatty acid esters to be tested in 99.35 g of reagent grade isopropanol. Four grams of each fatty acid ester solution was applied to the outer surface of each of the two hemostatic plugs to be tested to provide a treated hemostatic plug containing a 5% by weight ester or ester mixture, and an untreated hemostatic plug to be tested The weight S is the benchmark. Under 70¾, the alcohol was removed by evaporation, and then the treated hemostatic plug was tested according to the hemostatic plug method described in Example 1. The following is a clear list of fatty acid esters evaluated (please read the precautions before filling out this page). Packing. Line. The Ministry of Economic Affairs, the Ministry of Economic Affairs, the Central Bureau of Industry and Commerce, the cooperation and the printing of the content of ST. This is It is 9 in total. Acid-acid mix 36 oil octene decanoglycan β 0 0 0 1 ¾ 61 acid ester from oil fatty acid from glycerin octanoic acid and% co decorated gui ο with its gl about. Ester 3 And the original oil. The original 3868 Gan 硪 5K about acid and acid dip with decene 810, containing ¾ single with a medium S 1 ΊΛ plug: blood single stop 81. 4. 10,000 sheets' (H) The paper size is easy to use a Η Family Bibi (CNS) T4 specification (210X297 mm) L ㈣ L. 'Λ 6 ____ 5. Description of the invention () The rest of the mixture contains a small amount of diesters and triesters of these two fats. Hemostasis No. 2-90-95% purity glyceryl monolaurate, and contains 0.2% free glycerol and a small amount of diesters and triesters. Lauric acid is a saturated fat hemostatic plug & No. 3-90-95% pure glyceryl myristate containing 12% of atoms, and contains about 2% free glycerin and a small amount of diacetate and triester . Meat S · kolic acid is a fatty acid containing 14 mega atoms. Hemostatic plug No. 4-90-95% pure glycerol monopalmitate, containing 0.2% free glycerin and a small amount of diesters and triesters. Palmitic acid is a fatty acid containing 16 atoms of bright atoms. Hemostatic plug number 5-90-95% pure glyceryl monostearate, and contains 0.2% free glycerol and a small amount of diesters and triesters. Stearic acid is a fatty acid with a run of 18 to nitrate atoms. The Ministry of Economic Affairs's quasi-preparation: Gongxiaoyao cooperatives printed rt ,, 4 tampon No. 6-9 0-95% purity oleic acid oleic acid esters, and contains 〇. 2 _% free state glycerol and less M Diesters and triesters. Oleic acid is an unrun and fatty acid containing 8IS bright atoms and a double twist. In Example 8 of this book. Pseudo-use of two unmanageable hemostasis is used as a pair 81. 4. 10,000 sheets (H) (please read the precautions first and write this page) This paper is used for 5 good standards China National Standard (CNS) T4 specifications (210X297 male dragon) Λ 6 _Π6_ V. Description of invention () According to the object. The test results are shown in Table 8. This data shows that when compared to the amount of TSST-1 produced in the presence of an untreated control hemostatic plug, in the presence of a hemostatic plug treated with different fatty acid esters, the golden yellow «5 Staphylococcus species FRI-1169 The amount of TSST-1 produced was significantly reduced. The reduction in the amount of TSST-1 produced ranged from about 90% to 99%, and in the case of a tampon containing glyceryl monostearate, a 60% reduction in TSST-1 production was observed, although it did not They are as high as those obtained with hemostatic plugs containing other esters, but they are still very large, so they are considered very obvious. No corresponding reduction pattern was observed in the number of surviving golden yellow vine cells. However, it should be noted that at the end of the 24-hour incubation period, there were fewer surviving S. aureus cells on the treated hemostatic plugs than on untreated hemostatic plugs. (Please read the notes beforehand to write this page) Sun Yat-Sen _ Line · Line · Ministry of Economic Affairs, Central Bureau of Economic and Social Development, A Gongxiao Zuo Cooperative Cooperative Printed 3i 5 2 This paper is used in the BB home sample (CNS) > F4 specification (210X29 / male; «:) 81. 4.〗 0,000 sheets (H) V. Description of invention () I 丨 Isss Λ 6 Η 6 b Z · 09 £, 68『 96 T, 66 6 · Β6 (%) Fallen? ： I-lSSi State liM-ffl ^: 爼 刼 -N (69u Iocitt-) ® goods 泄 泯 Unloading the bottle, shipping, shipping, and chrysanthemum twist 03K · 000 (ε ^ 绍?: I-1SS1 £ ^ system ^ (q 趹 鸹 兹 idine ?: s 泜 倜 人! · 卜 τ

To ε ·· 1 · ^ L s ST (Please read the precautions first and fill in this page) ro0I H S.9 m0T Μ®0 · 9 g〇T x ^ · Τ-®οτ Μ s-ς 90T Ms · 1 " 9〇T Μ 0B · 10 5〇I Η Σ (4i w \) (太 焐 \ fu3) s * m fB-InIs TB 9L rs9 Du Yin made UDsssffil ^ T · " In 峨 明 勧 匧 匄 V 甥 拗 岧 qr ε 尨 琎 Mixed evenly: JT Ί installed 3 pastes. -^^ &-/ > 2 Bu :: 1 $ ^^ 邳 鬲 踗 踣.. : Ι} Μ0 ιβοι collar 2.恻 ί® (Υ 織 ”= ί s) ^^ v S 113 ^ (π loaded. Thread. 6 2 This paper scale is used in the middle-sleepy home sample rate (CNS) T4 specification (210x297 g; a :) 81. 4. 10,000 sheets (Η) V. Description of the invention (9 cases are verified in the report and verified in the use of A 6η 6 oleic acid laurel laurels-绖 司 合 公 混 在 此 d 1C 者 " The index comes from the income, and the cultural relics are as good as 0 ester in oil id glycine 1C acid Ur Gui La month for the second standard ester factory a two ears ¾ amount of two from 5 get 3 pseudo and, ester oil The mixed acid ester of osmanthus osmanthus osmanthus oil with the acid content of laurel laurel laurel 3 9 0 with vinegar and oil, now the vinegar is separated from the laurel vinegar to source from him

ο ο V ο w C e SH s 1 e The country κ is a self-purchased business system,,, Si Yigong's academic compound η mix pa I e kind t this se S Γ e substance mix this one α ¾e fink Heavy He 7 3 is obtained from ester oils. Glycolic acid-Gui Guiyue's single compound% combined mixed weight is 50%. The amount of glycerin-containing acid in ester-containing crude oil was increased to 6 3 9. The amount of glyceric acid in the oil-containing ester was 0-February L1 di 0-to-9. 1S was not U η 0 no ester. Ho oil is a famous name for sweet and sour, and it is prepared for two copies of 0 in the month. The blood is blocked. Stop rfy_J for the same phase. In 1 case .. and the general description of stop sequence blood flow stop% i% ο δ * ο 1 base, 1 and 1% and% s% 5 15 • so ir o 、 blood, %% ΊΑ 1 1i o: ¾ o to be included Ht Hv DC5 to be treated separately in each place, after being blocked, the blood is not blocked. Stop, stop U a mixed ester ο CSS e (please read -ift's notes on the back of this page * write this page) The Ministry of Economic Affairs Standards Bureau A Gongxiao Yang Cooperative Society printed 3i standard for S 1 sting and blood ¾ blood 5 Zhi ο0 Temple <n of ο There is no reason to contain the classics, each of which has a hemostatic percentage of ¾ ο η and quasi, for the blood although the Hr ’s hemorrhagic stop is measured} What is the reason for the ester to be contained In the raw material, its / IV substance is fermented with a mixture of 12 and -L and 90 is used to prepare two preparation methods. (J flTr 箜 9 The description of the blood table ends at the end of the test. The test paper is 0 0 and the root test. And the sample is copied to the promising copy. 7 2 The paper scale is based on the Chinese standard (CNS) ) Ή specification (2 丨 0x297 g; «:) 81. 4. 10,000 sheets (Η) V. Description of invention (^ τ13 辁 I-isss (%) Β6ςε

66 灯 6 CS 66 66 96 ® m Han · Ν \ Μ 1S3: ^ pm operation system ① 葙 κ m Zhao «Είπ 胡 轾 Η 坻 尾 κς AZ New 7 u 2. Cz- sister — 擗 WSSl e Hu Zhiyu ^ 6 * 69 vocational building) 0 「τ 〇9 · Em 3 · ^ 6ε · 0 ε6, ε 6 '^ 9Τ ·, 〇9Ε · 〇 (please read the precautions Sun filled in this page first) I t- fe ¢. S · /. (Zhuoline \) BU 01 MB, e ^ w \ Π d3) Pack · Z9 · L ° τ · s sz. IT · /. We 1 · ^ ες · Α_ου order- Line-Ministry of Economic Affairs, Central Standards :: Γ Duan Gongxiaoji cooperation Du Yinyu% 0 · ^ ^ 0 0 · 0 * A 6 paper scale edge used in a Η home standard Bi (CNS) Ή specifications (2 丨 0χ · 2!) Μ ;! 〇hoTHW ^ 〇 卜 οτ Μ 9τ '^ ς · 〇9〇Τ Ms _ Τ Τ. 0

£ 〇τ Μ "ε 卜 〇τ Μ" X 卜 〇Τ H "T ο, τ men _ ο τ, 〇 卜 〇1:« inch 芡 门 0 * ^ 卜 οτ Μ 91 'ς · 〇B〇TM s · Τ Τ · Ο

Avoid S carrying culvert type blowing w oussaM 婼 型 嘗 * U-Hp τυ · Μ: Ι η ρ q; ζια-06 S 1; nuioco2 81. 4.] 0,000ft (Η) A 6 _Π6_ V. Description of invention ( )

Kessco * contains 50% by weight of glyceryl monolaurate.

LaU "icidin * contains 93% by weight of glyceryl monolaurate.

Hon6Su] s 90-L12 contains 96% egg glycerol monolaurate. a) Measured by E L 1 S A method (R e i s e r et al.). b) Bottom 10 g value. All measurements were carried out after 24 hours at 37 hours. All the above data are the average of two samples. All the measured values. From the test data shown in Table 9, it can be seen that for any given degree of ester mixture in the tampon (that is, the added amount is 0.1%, 0.5% or j 1.0%), a lack of TSST occurs under the test conditions The amount of -1 is inverse tb with the turbidity of glyceryl monolaurate in the ester mixture. Therefore, for example, if the amount of the ester mixture in the hemostatic plug to be measured is kept fixed at the level of 0.5% addition, the final TSST-1 amount is pseudo 33.6 0. Chuck, and the ester mixture contains 50% by weight of glycerol monolaurate Ester (Italian KesscoO, decay to 3.39 gram, at this time the ester mixture contains 93% by weight GHL (meaning Lau "icidinO, and the speed is 0.36 gram, at this time in the ester mixture (please read the back attention first Matters Sun Yao wrote this page) -Installed · Ordered-Line ·. The kind of declining disease is not} 12 of the three likes and its kind of -1 oil -L is present iSTa · 90 丨 fiffiTS from ibb, oil-made raw 1S roof Gan Yiyan ouIr Fei acid in the bow ηοί-l Gui on a HO% ST month-there is that o T single} Contains-Krypton 1 after the base of its 29 ί and most referred to hydroxy ί I ester ¾ of the fruit should be ester oil 1 Result &gt; Reverse oil Gan ο production shows that it is not sweet. The effect of the acid used in the Chinese oil Gui has a month, 9 Gan Yue more single ridge table from the second} Pseudo-hydroxyl is mixed in, mixed with sleep and low in esters. Anti-Ministry of Economics, Biyang Bureau of Economics, Translator Consumers Cooperative, printed this paper. The paper is used in the SHS standard (CNS) T4 regulation (210x297 tf) 81. 4. 10,000 sheets' (Η) Λ 6 Β6

❾❾W Fifth, the description of the invention () (please read the precautionary code and fill in this page first) g Example 1 0: In vivo activity of the hemostatic stopper immersed in glyceryl monolaurate Split into the hemostatic stopper to be tested as described below. Use Avtex Xuanyue (100¾) «SH 2 5 ί 7 Sandani as the dimension to be tested. This secondary dimension was washed to remove T w e e η 20 and left unfinished, or coated with glyceryl monolaurate (Henkel Mononuls L-90) (hereinafter referred to as &quot; GHL &quot;). The analytical value of the content of monolauric acid in this substance is 96.0%, 2.0% 1-3 dif ester and 2.0% unidentified substance. Apply this thread as follows. Load 75 pounds of glaring stalks into a fixed tank. This tank contains water (120 gallons in total). Add ammonia (HH3) (29.4¾ ν, / ν) to the water in the fixed tank. Then heat the system to 200 Τ, after 30 minutes of ammonium. Then wash it with hot water (150 Τ) three times to determine whether there is any residual foam in the wash water. This foam proves that Tween 20 exists and then wash the cone with cold water (60 Τ). After Τϊζ: Ministry of Standards Bureau EΪ: Industrial Consumer Price Cooperative Innu, transfer this Xiaowei to a centrifuge and spin it for 5 minutes while hot to remove the fi water. This 75-pound Xuanyu initially contained 54 pounds of water. Then open the dazzle with your hand and put it back into the fixing slot. Place two restricting plates on the miscellaneous halves to reduce the spurting partner, and the effect and the generation of pimples are minimized. Add hot water (10-200T), then add four 5 lb GML test warts to the pan, each dissolved in five gallons of 1 70 T water. The system was pressurized and heated to 190 ° C, and the 30 minute mirror was recycled. After draining the system, take Xie Fei to the centrifuge and spin it for 5 minutes. At this point, 52 pounds of water (70%) is left. The temperature outside the birthday is 160 T, but the temperature inside the rich dimension is 175-180 T. Place the moist Xiewei in a belt oven. Heat it to about 2 5 0-2 6 0 1F. -30-81. 4. 10,000 sheets (H) SB home standard (CNS) TM specifications (210x297 g; «:) Λ! |! 卜 • Draw &gt; f? Ί1- / ;; Zuo Du印 f ^ V. Description of the invention (This heat treatment will cause the dazzle and comb without surface treatment to form 2.30 grams which can be washed in 盗 雒 to manufacture 2.30 grams and fixed at a compression of 0.62 inches in outer diameter and sealed. The control is named as (X). 2.75 English hours and 3. Directions, the length is gathered or shredded to cover the gadolinium and covered. For the Enka two-component sintering aid, it is coated with 2. Use manual ironing Mountain industrial length. The end of this string is 5/8 inch, thinly rolled, to test each shrink (side to side, then second ammonium. After compression, (0.62 inch outside The fiber of the diameter of the purple silk is used for the net or coated hemostatic plug. In the wrinkle. The blood plug of the applicator is marked as follows. The width of the machine is 0 inches. The machine is made. The cloth is heavier than the control blank (2 . 7 5 &quot; X 4% EHL bucket, pass 8/5 of the cover through each blank and then wind into the gray cloth ~ the opposite end of the anchor's back end will stop immediately.) The pull step is opened and the lotus line passes through the fiber belt, , Drying and will This 曡 雒 for five seconds. Up. The blood is (y), it is the segment of the blood clot, facing, 2.28 grams to the amaranth. — For the cloth, 4. 7 5 &quot;) 〇 The solution 0. The cover itself One end circle of the white dazzling cloth. Yu Dingding strength.). Re-fix the blood ridge and put it into a thin frame and dry it -31-Λ 6 Π 6. Then apply the R and 〇 web forming machine, and then make it After combing. Use this dazzle to compress it in two directions, after compression, put the tamper to be wrapped in cellophane paper, and GHL-coated hemostatic plug: by cutting this dazzle to make _ Into a grey cloth. It is horizontal in terms of dimension. The area is then covered with a 0.25 cup of Xuanyu section. For EML gray cloth, its 25 cup of Enka two-component heat seal. From B 1 ue Fine®, cut into 13.0 inches, after a picture of one of the through units is connected, connect this to the cloth in two directions and fix it to the 5 R eggie three joint coating The gripper is not knotted. Pack the hemostatic plug (please read the precautions first and then write this page). This paper is used in the "CNS" specification (210x297 ) 81. 4. 10,000 sheets' (H) V. invention is described (Λ 6 Π 6

The Ministry of Economic Affairs # Zhuo Er ft work consumer cooperation Du Yin M and sealed in white celluloid sleeves. The compressed hemostatic plug was analyzed to determine the average concentration of GML on the hemostatic. The average concentration of GML on the tested hemostatic plug was 2.38%. Compared with the hemostatic stopper with unfinished surface, the in vitro impact of this GHL hemostatic stopper was evaluated using the Holland vibrating beaker method and the Reiser hemostatic bladder method used to evaluate the golden yellow meal ® to produce T S S T-1. These methods have been described in Examples 1_ and 7 above. The impact of GML (2.38¾ w / w) coated hemostatic plug is shown in Tables 10 and 11. Table 10 shows that when the hemostatic plug was evaluated using the vibrating edge beaker method described below, it was found that there was a greater than 99.9% drop in TSST-1 generation @: A two-liter beaker with three blades was sterilized in an autoclave, where Contains 500 ml D ί fc 〇 Brain heart injection medium. After sterilizing the killifish, add five milliliters of Golden Yellow Grape Ball® (marked as U87) after 24 hours of cultivation and add it to the beaker. Add 25.0 grams of the substance to be tested to the beaker, or add no substance (in the control beaker), in two portions. All beakers were cultured at 37t: and shaken at 160 ° F for 24 hours. At this time, the TSST-1 concentration and the number of golden yellow cells were measured. The TSST-1 content was determined using the ELISA test, and the number of striatal cells was measured using a standard plate counting procedure. It can be seen in Table 11 that using the hemostatic ridge method, Sulian GML hemostatic ridge and the untreated surface hemostatic ridge to the golden yellow jealous word ball. Among them, the reduction range in the production of TSQ-1 is from 81.1% when using blood in Peleiki to 95.9% when not using blood, but for 32-the paper size Used in 11 sleepy homes (CHS) T4 regulations (210x297 male dragons) 81. 4. 10,000 sheets' (H) (please read the back-end notes # fill out this page)-Pack. Order_ Line-Economy The Ministry of Finance, Industry and Consumer Affairs, Du Yin λ'14 Λ 6 Π 6 V. Description of the invention () The impact of the golden and staphylococcus aureus cell count is one point in the presence of blood. Collar, or 9.1% of the test tubes that do not use blood. ‘Internal evaluation of its efficacy. 傜 proceeds as follows. The control and GML hemostatic plugs were mailed to the Southwest Research Institute in San Antonio, Texas, to evaluate the reduction effect of TSST-1 produced by Golden Globe® in the vagina. Using amine HC1 immobilization, identify twelve female whiskers, and vaginal examination to obtain an evidence of infection. The control hemostasis three without surface finishing, at 37X: After 24 hours, 5 ml of Staphylococcus aureus p sex strain grows in the brain-heart perfusion medium and is absorbed at its end (away from the ends of the fun) ). Immediately place the pre-weighed inoculation hemostatic plug into the vaginal vagina without using an inspection scope, and remove it and pull it back to use a thin lump. Measure and record the direct temperature and indirect systolic blood pressure. Five milliliters of blood samples were taken from the blood vessels of the head, and the serum was stored at -7〇υ until the analysis of anti-TSST-1 antibody appeared and clinical chemistry could be performed. Keep the vaccination tampon in the vagina for twelve hours. Twelve hours after the first fat, the amine ketone H C 丨 was used to fix the brush and the tampon was removed. Place the inoculated hemostatic plug in a 4 ounce plastic if cup that has been previously stung. Weigh the tampon and cup together. Calculate the egg that accompanies the tampon. Transfer this hemostatic material to a stomach bag containing 50 ml of saline (0.9% Ha C1), and mix for 60 seconds. Then, determine the value of the flow in the remaining bag, to determine the value of the gold member's color and the T S S Τ-1 jg degree. The number of running cells is determined by using the standard plate counting method for this paper-scale homelessness (CNS) rule (210x29 / mm) 81. 4. 10,000 ». (H) (please read on the back Note: Please fill in this page again) Install · Line. Λ 6 1J6 The Ministry of Economic Affairs, Bureau of Standards, Industry, Consumers, 1Ϊ · Cooperative Print 51 V. Description of invention (), and TSST-1 degree, use radioimmunoassay (R.I.A.) determination. All hemostatic plugs inserted after inoculation of hemostatic plugs will be handled as described above. ίΕ After removing the inoculated hemostatic plug, all whisks were inserted into the vagina as a control (y) hemostatic plug to allow the growth of additional golden yellow bulbs and the production of TSST-1 in the vaginal cavity. After another twelve hours of incubation, the animals were divided into two groups of six animals, six of which were tested with the control hemostatic plug, and the other six were tested with G M L coated hemostatic plugs. 4 After the 8-hour exposure period, all animals that have been inserted into the stopper are supplemented with their own 5.0 ml of serum, because the flow of Yue Ti is reduced. The number of viable Staphylococcus aureus cells and the content of TSST-1 were measured on all hemostatic plugs. After all hemostatic plugs have been completed and toxin and cell count measurements have been performed, four pairs of animals were excluded from the study (two control animals and two GHL animals to be visited). In these animals, the organic neck has not moved into the vaginal cavity to cause infection, or it has been found to have less toxin or less cellular content that tb is known to have been applied to the vaccinated hemostatic plug. Table 12 shows the toxin data per ml of fluid accompanying the tampon and the toxin data on the tampon, and their impact on cell count. The data shown are represented by four animals in each test group. The numbers shown in Table 12 fell nirvana. In the four animals with the GHL hemostatic plug, there was a considerable reduction in toxin formation compared with the control hemostatic test. Figures 1-4 show the number of GHL hemostatic effects. Although at the beginning of the reading, the content of sucralose in the sample with control hemostatic ridge is higher than that of TSST-1, but GHL hemostatic ridge makes the dentin content not obvious -34-(please read the back And the precautions # fill out this page) Install &Order; Line-This paper is used in the national standard (CNS) Τ4 specifications (210x29 / mm) 81. 4.〗 0,000 sheets (Η)

V. Description of the invention () The chopsticks are lower than those found in the control. This data is representative of the toxin produced by each 106-gram Staphylococcus aureus cell, so the data is normalized to the diabetic cells, which confirms a significant reduction in hemostatic plugs containing G H L 'compared to the control hemostatic plugs. After adding blood to the hemostatic plug for 60 hours, it was found in control animals that the toxins associated with bacterial cells had an increasing trend. The trend found in control animals seems to be a direct impact on the cell's ear and growth curve. This trend is also found in animals with GHL hemostatic plugs. The data shown in Table 13 shows the comparison of the tb control percentage of j toxin on the flow plaque with the tampon, and the comparison of the detoxification factor formed on the tampon with the control tampon. (Please read the back and the precautions # fill in this page first) Pack-line. Economically determined standard and εί: printed by the industrial and commercial cooperatives Μ 5 3 This paper 5fc standard side uses the Chinese SS home sample (CNS) Ή specification (210x29 / Public: «:) 81. 4. 10,000 sheets (Η) Π 6 V. Description of invention () isi ± ^. ^ Ifov110H ^ 3! ^ Els · ® A! ^ 4HI-lssi ^ M ^ TIJrqT ^^ :. «) LJHD01 w 2TS Μ 02.τ (Π3.) Ss? Lf \! Lis 泜 聛 涵 昍 5〇Ι Η U {朱 架 / = JQ) made of Faz-N bottle Hueffi birch (%)

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Ίκ ° TSO ΤΚ3 TSO TSO 眭 · ε3 60IXS6.I yoke control lowering cabinets barium moe filling _ 绡 岧 ^ w / -D-Ixg.s ^^-^^ M- ^ • Seeking w / nfc 80IX- -Yuan Pi zkf, 菪 亪 bandits- 辄 • Net and 彐 女 忤 0 (please read the notes beforehand Sun fills in this page) 装-定 _ 线 · This paper is used in the standard a Η 家 橒 准(CNS) T4 specification (210X297 male dragon) 81. 4. 10,000 sheets (Η) LOGbC- Λ 6 ___HJ5 V. Description of invention () Table 13 Control percentage of toxin formation (please read the precautions to be filled first Page) Pack. Thread · Line · The Ministry of Economic Affairs, the central government ^; β work consumer cooperation in cooperation with Du printed 9 3 type of tampon to the control of the toxin formed in the toxin formed with the tampon on the tampon Percentage of control in bb bb t (%) ⑴ Control animal control--Control 199 * 294 Control 134 -10 2 Control 29 40 Control 56 77 Control 4 1 119 Pending animal control--* G HL 46 30.5 G HL 33.! 20. 25 paper 5t scale edge Used in the middle of the family 揣 榣 (CNS) T4 specification (210X297 public release) 81. 4. 10,000 sheets (H) L05bG-i A6 l &gt; Ό V. Description of invention () Table 14: Department of Economics, Ministry of Economic Affairs, quasi-d: Gongxiao &quot; Cooperative Innu ’s various fatty acid ester compounds have an impact on growth * and the formation of TSST-1, the MTSS Τ-1 produced by the golden yellow grapes, and the final sample of TSST-1 produced on the ‘TS’-1 The absolute reduction of the logarithmic value (/ ml> (Chuck) (%) inoculation 1 169 11.0 -J 15.45-control hemostatic plug 9.60 8.566-hemostatic plug 1 0.1: 3: 9.1 7.93 7% ϋ. 5¾ 7.64 1.25 85% 1. 0¾ 8.79 0.09 9 9% 10¾ .8.37 0.05 99¾ tampon 2 Ψ 0 · 1% 8.45 0.12 99% 0. 5% 7.40 0.01 99¾ 1. 0% 6.13 0.01 99¾ 10¾ 7.42 0.11 99¾ (please (First read the back-to-back precautions Sun Gou wrote this page) This paper uses the Chinese poverty-stricken family (CHS) rules (210x297 male dragon) 81. 4. 10,000 sheets (H): n Λ 6 Π 6 Ming Explain that five cases of dyslipidemia and fatty acid RH were not included in 11 cases. To

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Adults, raw species, 1 c si- its species and the test of the growth and death of blood plug 9) I will be used in 16 (F in the female 1 ball example, the grape color is yellow and yellow with gold for the -1 set ST g'J-TS is not included in the preparation, the blood flow of the stopcock sequence is generally measured. 1 To be described, the ester preacid, the fatty acid, the fatty acid, the root, the root 10 0 and the middle 0¾ il · 1Λ 11 cases, the actual 5X is less than 、 Use 1 system ο The fat in the fat 1 Any example is based on the fact that it contains two quasi-use bases that are not combined. The test is made by measuring the volume of the blood. The statement is summarized in the report. Test 0. The shed works to stop the blood clots at 61. Acidic (please read the back-end notes #Item to write this page) Binding-Order-Line · Ministry of Economic Affairs屮 央 #Public Bureau β 工 消 汾 Cooperation Duyin 3i 11 4 This paper scale is used in the middle S Η 楳 楳 Bi (CNS) regulations (210x297 male dragon) 81. 4. 10,000 sheets (Η)

Claims (1)

Secret Announcement A 7 B7 C7 D7 VI. Application for Patent Fan Ji Patent Application No. 80 108379 ROC Patent Appln. No. 80 108379 Amendment to the scope of the patent application Chinese Amendment 1 Amend Claims in Chinese-EncI. I (Amended and submitted on October 21, 1981) (Amended & Submitted on October 21, 1992) Patent application for amended & amended: 1. A liquid composition for women ’s personal hygiene, which contains a pharmaceutical acceptable carrier And a compound selected from the following groups: (a)-a polyhydroxy aliphatic alcohol and a monoester moth containing eight to eighteen atoms of fatty acids, wherein the monoester has at least one hydroxyl group and its wax alcohol residues Phase association; (b)-a polyhydroxy aliphatic alcohol and a diester containing eight to eighteen atoms of fatty acids, wherein the diester has at least one hydroxyl group associated with the residue of the aliphatic alcohol; Μ and (c) the mixture of monoesters and diesters, the above compounds are present in an amount effective to inhibit the production of toxic shock syphilis virus-1 by the bacteria when the composition is exposed to Staphylococcus aureus. 2. The composition according to item 1 of the patent application scope, wherein the amount of the compound present is at least about 0.1% based on the weight of the composition. 3. The composition according to item 1 of the patent application scope, wherein the amount of the compound present is at least about 1.0% based on the weight of the composition. Printed by the 8th Industrial and Consumer Cooperative of the Central Bureau of Standards of the Ministry of Economic Affairs (please read the precautions on the back before filling out this page) The weight of the composition is at least about 10%. 5. The composition according to item 1 of the patent application scope, wherein the fatty acid is laurel. 6. The composition according to item 1 of the patent application scope, wherein the polyol is glycerin. 、 This paper scale is applicable to China National Standard (QjS) A4 specifications (2 丨 0x297 public goods) ^ ObbC ^ a7 B7 C7 ------------ D7 _ VI. Patent application Fan 7 . The composition according to item 1 of the patent application scope, wherein the compound is glyceryl monolaurate. 8. The composition according to item 7 of the patent application scope, wherein the amount of the glyceryl monolaurel vinegar present is at least about 0.1% based on the weight of the composition. 9. The composition according to item 7 of the patent application scope, wherein the amount of the glyceryl-laurate present is at least about 0.5% based on the weight of the composition 1 10. The composition according to item 1 of the patent application scope , Wherein the compound contains a mixture of glyceryl monolaurate and glyceryl dilaurate. 11. The composition according to item 10 of the patent application scope, wherein the amount of the mixture present is at least about 0.1% based on the weight of the composition. 12. The composition according to item 11 of the patent application scope, wherein the mixture contains at least 90% glyceryl monolaurate by weight. 13. The composition according to item 10 of the patent application scope, in which the amount of the mixture is based on the weight of the composition • at least about 0.1%. 14. The composition according to item 1 of the patent application scope, wherein the compound contains glyceryl monocaprylate. 15. The composition according to item 1 of the patent application scope, wherein the compound contains glyceryl caprate. 16. The composition according to item 1 of the patent application scope, wherein the compound comprises a mixture of glyceryl-caprylate and glyceryl caprate. . Printed by the Beigong Consumer Cooperative of the Central Bureau of Economic Affairs of the Ministry of Economic Affairs (please read the precautions on the back before filling in this page) • Line · 17. The composition according to item 1 of the scope of the patent application, in which the compound contains glyceryl-based meat Myristate. 18. The composition according to item 1 of the patent application scope, wherein the compound contains glyceryl monopalmitate. 43-This paper scale is applicable to the Chinese National Standard (CNS) Grade 4 (210X297 mm) A 7 B7 C7 D7 Printed by the Central Sample Bureau of the Ministry of Economic Affairs, Cooperate with Consumers of DuPont "6. Application for a patent model 19. According to the application The composition according to item 1 of the patent scope, wherein the compound contains glyceryl monostearate. 20. The composition according to item 1 of the patent scope, wherein the compound contains glyceryl oleate. A liquid composition for women's hygiene, which contains a pharmaceutical acceptable carrier and a compound selected from the following group: (a)-a polyhydroxy aliphatic alcohol and a fatty acid containing 8 to 18 atoms of fatty acids Monoesters, wherein the monoesters have at least one hydroxyl group associated with the residue of the aliphatic alcohol; (b)-a polyhydroxy aliphatic alcohol and a diester of fatty acids containing 8 to 18 atoms Where at least one hydroxyl group of the diester is associated with the residue of aliphatic alcohol; Μ and ί (c) the mixture of the monoester and the two g compounds, the amount of the upper compound is present when the composition is exposed to . Staphylococcus aureus can effectively inhibit the production of the bacteria Enterotoxin A, Cotoxin B, or Enterotoxin C. 22. The composition according to item 21 of the patent application, where the amount of the compound is present · based on the weight of the composition, at least about 0.1%. 23. According to The composition of claim 21, wherein the amount of the compound present is at least about 1.0% based on the weight of the composition. 24. The composition of claim 21, the amount of the compound present, According to the weight of the composition, at least about 10%. 25. The composition according to item 21 of the patent application, wherein the fatty acid is lauric acid. 26. The composition according to item 21 of the patent application, wherein the polyol It is glycerin. 27. The composition according to item 21 of the patent application scope, in which the polyhydric alcohol is glyceryl monolaurate. -44-(Please read the notes on the back first and then fill this page) •, Λ_ • Thread ·. Line · This paper scale is in accordance with the Chinese National Standard (CNS) A 4 specifications (210x297 mm) AB c D / 、, patent application Fan Garden 28. The composition according to item 27 of the patent application scope, in which the glyceryl group One laurate exists , Based on the weight of the composition, at least 0.1%. 29. The composition according to claim 27, wherein the amount of the glyceryl monolaurate present, based on the weight of the composition, is at least 1.0% 30. The composition according to item 21 of the patent application scope, wherein the compound comprises a mixture of glyceryl-laurate and glyceryl dilaurate. 31. The composition according to item 30 of the patent application scope * wherein the compound The amount present * is at least about 0.1% based on the weight of the composition. / 32. The composition according to item 31 of the patent application, wherein the mixture contains at least 90% glyceryl-laurate by weight. 33. The composition according to item 31 of the patent application scope contains at least 95% glyceryl-laurate 34. The composition according to item 31 of the patent application scope, based on the replacement of the composition, is at least about 0.1%. 35. The composition according to item 34 of the application scope, wherein the mixture contains at least 90% glyceryl-laurate by weight. 36. The composition according to item 35 of the patent application range contains at least 95% glyceryl-laurate 37. The composition according to item 21 of the patent application range wherein the mixture is by weight of the mixture in which the mixture is present The compound contains Gan by weight (please read the precautions on the back and then fill in the copy). Oil-based-octanoate printed by the Central Sample Bureau of the Ministry of Economic Affairs Printed by Beigong Consumer Cooperative> 3 /. According to the patent application The composition of Item 21, wherein the compound comprises glyceryl caprate. 39. The composition according to Item 21 of the patent application range, wherein the compound comprises a mixture of glyceryl monocaprylate and glyceryl caprate. 45-The scale of the table paper is in accordance with China Gujia Standard (CNS) Jia 4 Woven (210x297 public goods) AT B7 C7 D7 Six 'patent application garden 40. The composition according to item 21 of the scope of patent application, in which the compound contains glyceryl 1. Myristic acid vinegar. 41. The composition according to item 21 of the patent application scope, wherein the compound contains glyceryl monobranched acid ester. 42. The composition according to item 21 of the patent application scope, The compound contains glyceryl monostearate. 43. The composition according to item 21 of the patent application range, wherein the compound contains glyceryl-oleate. 44. A liquid composition for women ’s hygiene, It contains a pharmaceutically acceptable carrier and a compound selected from the following groups: (a)-a polyhydroxy aliphatic alcohol and a monoester of fatty acids containing eight to eighteen atoms | wherein the monoester has at least one The hydroxyl group is associated with the aliphatic alcohol residue; (b)-a polyhydroxy aliphatic alcohol and a diester containing eight to eighteen fatty acids, wherein the diester has at least one hydroxyl group and its aliphatic alcohol residue Phase association; Μ and (c) the mixture of monoesters and diesters, the above compounds are present in an amount that is effective when the composition is exposed to A, B, F and G Streptococcus Bacteria produce Streptococcus heat-generating exotoxins A, B, CM and hemolysin. Ministry of Economic Affairs Central Standards Bureau Bei Gong Consumption -46-(Please read the precautions on the back before printing this page) Printed by the cooperative-this paper size is applicable China Home Standard (CNS) f 4 Specifications (210x297 mm)