TW202309495A - 將生物材料透明化的方法及套組 - Google Patents

將生物材料透明化的方法及套組 Download PDF

Info

Publication number
TW202309495A
TW202309495A TW111115474A TW111115474A TW202309495A TW 202309495 A TW202309495 A TW 202309495A TW 111115474 A TW111115474 A TW 111115474A TW 111115474 A TW111115474 A TW 111115474A TW 202309495 A TW202309495 A TW 202309495A
Authority
TW
Taiwan
Prior art keywords
reagent
biological material
reagents
less
located inside
Prior art date
Application number
TW111115474A
Other languages
English (en)
Chinese (zh)
Inventor
成相哲朗
Original Assignee
日商住友化學股份有限公司
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 日商住友化學股份有限公司 filed Critical 日商住友化學股份有限公司
Publication of TW202309495A publication Critical patent/TW202309495A/zh

Links

Images

Classifications

    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00Sampling; Preparing specimens for investigation
    • G01N1/28Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00Sampling; Preparing specimens for investigation
    • G01N1/28Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/30Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
TW111115474A 2021-04-26 2022-04-22 將生物材料透明化的方法及套組 TW202309495A (zh)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2021074179 2021-04-26
JP2021-074179 2021-04-26

Publications (1)

Publication Number Publication Date
TW202309495A true TW202309495A (zh) 2023-03-01

Family

ID=83847026

Family Applications (1)

Application Number Title Priority Date Filing Date
TW111115474A TW202309495A (zh) 2021-04-26 2022-04-22 將生物材料透明化的方法及套組

Country Status (3)

Country Link
JP (1) JP2022168842A (ja)
TW (1) TW202309495A (ja)
WO (1) WO2022230750A1 (ja)

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2015049101A (ja) * 2013-08-30 2015-03-16 オリンパス株式会社 生体透明化剤
WO2017188264A1 (ja) * 2016-04-28 2017-11-02 国立研究開発法人理化学研究所 光透過性に優れた生物材料を調製するための組成物およびその利用
JP7033795B2 (ja) * 2017-02-22 2022-03-11 国立大学法人 長崎大学 生体由来材料の透明化用試薬
WO2018194253A1 (ko) * 2017-04-21 2018-10-25 한국화학연구원 생체 조직 투명화용 조성물 및 이를 사용한 생체 조직의 투명화 방법
JP7160350B2 (ja) * 2017-07-06 2022-10-25 公立大学法人大阪 生体組織透明化法及びその試薬

Also Published As

Publication number Publication date
WO2022230750A1 (ja) 2022-11-03
JP2022168842A (ja) 2022-11-08

Similar Documents

Publication Publication Date Title
Richardson et al. Tissue clearing
US10794802B2 (en) Whole-body tissue stabilization and selective extractions via tissue-hydrogel hybrids for high resolution intact circuit mapping and phenotyping
JP7384789B2 (ja) 大型組織の標識、透明化及びイメージングのための方法
Treweek et al. Whole-body tissue stabilization and selective extractions via tissue-hydrogel hybrids for high-resolution intact circuit mapping and phenotyping
WO2015022883A1 (ja) 光透過性に優れた生物材料を調製するための組成物およびその利用
Babaei et al. Contrast-enhanced X-ray micro-computed tomography as a versatile method for anatomical studies of adult zebrafish
JP7028979B2 (ja) 組織試料調製システム
US10359344B2 (en) Composition for clearing of biotissue and clarity method for biotissue using thereof
US10955322B2 (en) Methods and devices for soft and osseous tissue clearing and fluorescent imaging
Duchi et al. Protocols for culturing and imaging a human ex vivo osteochondral model for cartilage biomanufacturing applications
KR102085373B1 (ko) 3차원 형광 이미지화를 위한 생체조직 투명화 키트 및 이를 이용한 투명화 방법
WO2021015196A1 (ja) 生体組織の処理方法及び処理用組成物
US11391653B2 (en) Composition for immunostaining cleared large tissues and method for immunostaining cleared large biological tissues
TW202309495A (zh) 將生物材料透明化的方法及套組
JP6161074B2 (ja) 抗体組成物、抗体組成物調製用キット、及び免疫染色方法
WO2022102623A1 (ja) 生物材料を透明化する方法およびキット
Wilmerding et al. Expansion microscopy of the chick embryo neural tube to overcome molecular crowding at the centrosomes-cilia
Biswas et al. SUMIC: a simple ultrafast multicolor immunolabelling and clearing approach for whole-organ and large tissue 3D imaging
Aryal et al. Characterization of Astrocyte Morphology and Function Using a Fast and Reliable Tissue Clearing Technique
KR102644979B1 (ko) 생물학적 이미징을 위한 새로운 수성 굴절률 동기화 및 조직 투명화 용액
EP3994439A1 (en) Method for preparing biological material for microscopy analysis
Zelinger et al. Three-dimensional correlative microscopy of the Drosophila female reproductive tract reveals modes of communication in seminal receptacle sperm storage
Malloci et al. Label-free imaging of large samples: 3D rendering and morphological analysis within histological workflows using serial block face imaging
Katz SILVER LININGS: APPLICATIONS OF SILVER STAINING FOR
Wiesmann et al. Toxicological Analysis by Assessment of Vascularization and Cell Viability Using the Chicken’s Chorioallantoic Membrane (CAM Assay)