TW202222160A - Antimicrobial agent and method for manufacturing same - Google Patents

Abstract

The present invention provides an antimicrobial agent that achieves both an excellent antimicrobial effect and persistence at a high level, and includes a plant-derived antimicrobial component and a hydrolase.

Description

Antibacterial agent and method for producing the same

The present invention relates to an antibacterial agent and a manufacturing method thereof.

In order to prevent infection of bacterial or viral diseases, and to disinfect human hands that may come into contact with bacteria or viruses, or to disinfect furniture or daily necessities that may be touched by hands, it is usually sprayed on human hands, furniture or daily necessities, etc. Ethanol solution or hypochlorous acid water, or wipe with a non-woven cloth or cloth containing ethanol solution or hypochlorous acid water.

The problem to be solved by the invention

Although ethanol or hypochlorous acid water has sufficient antibacterial effect, the industry has not stopped seeking antibacterial agents with better antibacterial effect, and research and development are increasingly carried out. In addition, as for the ethanol solution, although it varies with the concentration of ethanol in the solution, its volatility is high, and it evaporates immediately when it is applied to, for example, furniture. Therefore, in a short time before the application of the ethanol solution to volatilization, the desired excellent antibacterial effect may not necessarily be obtained. Therefore, an antibacterial agent with a certain degree of persistence is desired; however, it is difficult for the ethanol solution, which is a conventional antibacterial agent, to have both excellent antibacterial effect and persistence.

The present invention advantageously solves the above-mentioned problems, and aims to provide an antibacterial agent having both excellent antibacterial effect and durability at a high level. means of solving problems

As a result of intensive research, the inventors of the present invention found that an aqueous solution containing plant-derived antibacterial components and hydrolytic enzymes can have both excellent antibacterial effect and durability at a high level, and can solve the above problems, leading to the completion of the present invention.

The present invention is shown in the following [1] to [10]. [1] An antibacterial agent comprising plant-derived antibacterial components and hydrolytic enzymes. [2] The antibacterial agent according to [1], wherein the aforementioned plant-derived antibacterial ingredient comprises at least one extract selected from the group consisting of mangosteen extract, horseradish extract and ginger extract. [3] The antibacterial agent according to [1] or [2], wherein the hydrolytic enzyme comprises at least one enzyme selected from lipolytic enzymes and proteolytic enzymes. [4] The antibacterial agent according to any one of [1] to [3], further comprising a pH adjuster. [5] The antibacterial agent according to any one of [1] to [4], further comprising an essential oil. [6] The antibacterial agent according to any one of [1] to [5], further comprising an antibacterial component other than the aforementioned plant-derived antibacterial component. [7] The antibacterial agent according to any one of [1] to [6], further comprising a cellulose derivative. [8] The antibacterial agent according to any one of claims [1] to [7], which is a transparent liquid. [9] A method for producing an antibacterial agent, comprising a step of adding a plant-derived antibacterial component and a hydrolytic enzyme to water or an aqueous solution of pH 6.5 to 10. [10] An antibacterial agent comprising menthol, xylitol and hydrolase. effect of invention

According to the present invention, an antibacterial agent having both excellent antibacterial effect and durability at a high level can be provided.

The form in which the invention is carried out

The antimicrobial agent of this invention and its manufacturing method are demonstrated more concretely. The antibacterial agent of the present invention contains plant-derived antibacterial components and hydrolytic enzymes.

The inventors of the present application found that an antibacterial agent containing both a plant-derived antibacterial ingredient and a hydrolytic enzyme has better antibacterial effect and better durability than an antibacterial agent containing a plant-derived antibacterial ingredient alone, The present invention has thus been completed. Each component is described below.

(antibacterial ingredients derived from plants) Examples of plants with antibacterial effects include mangosteen, wasabi, ginger, basil, houttuynia cordata, grapefruit, betta, cherry, mango, kiwi, etc. Grapefruit, betta, cherry, mango, and kiwi can also be selected. Using peels, fruits, or seeds, one or two or more kinds of extracts (hereinafter referred to as "extracts") extracted from these plants can be used in combination as a botanical antibacterial ingredient. Plants rich in flavonoids (collectively referred to as "flavonoids") such as mangosteen are preferred plants. In addition, xylitol or menthol derived from peppermint is also contained in plant-derived antibacterial components. The antibacterial ingredient which uses only the composition of the antibacterial ingredient derived from a plant can be regarded as an antibacterial agent with high safety to the human body.

The botanical antibacterial ingredient can be obtained as an extract by a well-known production method using the above-mentioned plants as raw materials. As a method for producing such a well-known extract, for example, a method for extracting an extract by immersing a plant body or scraps in water, hot water, or alcohol, and a method for extracting an extract by applying pressure to the plant body or scraps can be mentioned. , The method of crushing the plant body and dissolving the crushed material in water, hot water, alcohol, etc. In addition, among the above-mentioned plants, since several kinds of plants are commercially available as extracts, the extracts can be used as plant-based antibacterial components. Moreover, a commercial item can also be used for the antibacterial agent whose main component is a plant extract.

In the mangosteen extract, salty polysaccharides are the main components of the antibacterial effect. In the wasabi extract, allyl isothiocyanate is recognized as the main component of the antibacterial effect. In ginger extract, ginger oil or gingerol is the main component of antibacterial effect. In the perilla frutescens extract, perilla aldehyde, which is considered to be a polyphenol, is the main component of the antibacterial effect. In Houttuynia cordata extract, decanoyl acetaldehyde is considered to be the main component of antibacterial effect. Among the extracts of flavonoids, flavonoids are considered to be the main components of antibacterial effect. These extracts are also used in the form of food, and the common point is that the toxicity to the human body or the environmental burden is small, and they can exert excellent antibacterial effects.

Among the above-mentioned plant extracts, since mangosteen extract, wasabi extract and ginger extract have particularly excellent antibacterial effects, it is preferable to include at least one of these mangosteen extracts, wasabi extract and ginger extract .

Antibacterial ingredients derived from plants are preferably contained in an amount of about 0.01 to 0.1 mass % in the antibacterial agent in the form of an aqueous solution. When it is less than 0.01 mass %, it is difficult to obtain the desired antibacterial effect, and when it contains more than 0.1 mass %, the antibacterial effect is saturated. More preferably, it is 0.05-0.1 mass %.

(Hydrolytic enzyme) Hydrolytic enzymes are enzymes that catalyze the hydrolysis reaction. The representative reaction catalyzed by hydrolase is the reaction of digesting and decomposing protein, lipid, polysaccharide (carbohydrate) into amino acid, fatty acid, glucose, etc. The antibacterial effect can be improved by adding hydrolase, and the durability can be improved at the same time.

Furthermore, by containing hydrolytic enzymes, even without washing with soap or other detergents in advance, it is possible to remove protein or lipid biofilms that inhabit the habitats of fungi or viruses. Therefore, it is an antibacterial agent that can exhibit an antibacterial effect equivalent to or higher than that of ethanol, and even though the detailed reason is unknown, it is an antibacterial agent that has excellent sustainability.

Examples of the hydrolytic enzymes include proteolytic enzymes, lipolytic enzymes, and saccharolytic enzymes, and preferred are proteolytic enzymes and lipolytic enzymes. The reason for this is that proteolytic enzymes and lipolytic enzymes act on bacteria or viruses to decompose them. One type of hydrolase may be used, or two or more types of enzymes may be used in combination. The protease includes alkaline protease, neutral protease, acid protease, and peptidase. Lipase, phospholipase, esterase, and phosphatase are exemplified as lipolytic enzymes.

As the hydrolase, commercially available enzymes can be used. Examples of proteases include Orientase AY, Tetrase S (A feed), Orientase 10NL, Orientase 90N, Orientase OP, Nukureisin, Orientase 22BF, manufactured by HBI Corporation, Sumizyme AP, Sumizyme FP, Sumizyme LP, Sumizyme LPL, manufactured by Shin Nippon Chemical Industries, Ltd. Sumizyme MP, Denapsin 2P manufactured by Nagase ChemteX, purified papain for food, Denazyme AP, Bioprase 30L, Bioprase 30G, Bioprase AL-15FG, Bioprase APL-30, Protease CL-15, Bioprase SP-20FG, Bioprase XL-416F, Denazyme CPO PEPRICH, Denazyme PMC SOFTER, Brewers Clarex manufactured by DSM, Maxipro AFP, Akelazyme NP50.000, Maxipro NPU, Maxipro BAP, Bakezyme B500, Bakezyme PPU95.000, Maxipro PSP, Fromase, Maxiren, Newtlase 0.8 L manufactured by Novozymes Japan , Newtlase 1.5 MG, Alcalase 2.4 L FG, Protamex, Danisco Japan Multifect PR 7L, EFFECTENZ P, Optimase PR, Multifect PR 6L, Nakdong Chemical Industry Co., Ltd. ENZYLON SA, Magnux MT103, Toyobo Co., Ltd. ALP-101, Yakult AROASE AP-10, AROASE XA-10, AROASE NP-10, PANCIDASE NP-2, PANCIDASE MP, PANCIDASE P, Protease YP-SS, Mitsubishi Chemical Foods Co., Ltd. Kokulase P granules, purified papain, Amano Enzyme Thermoase PC10F, Newlase F3G, Papain W-40, Pancreatin F, Protin SD-AY10, Protin SD-NY10, Protease A "AMANO" SD, Protease M "AMANO" SD, Protease P "AM ANO" 3SD, Bromelain F, ADMIL manufactured by Contract Alcohol Co., Ltd., Mingtang Rennet, Mingtang Rennet Super.

Examples of peptidases include Flavourzyme 1000 L, Flavourzyme 500 MG manufactured by Novozymes Japan, ProteAX manufactured by Amano Enzyme, Peptidase R, Maxipro FPC manufactured by DSM Corporation, Maxipro CPP, and PSP-101 manufactured by Toyobo Corporation. The lipases include ENZYLON LP manufactured by Nakto Chemical Industries, Ltd., Sumizyme NLS manufactured by Nippon Chemical Industries, Ltd., Newlase F3G manufactured by Amano Enzyme Corporation, lipase R "AMANO", lipase A "AMANO" 6, lipase AY "AMANO" 30SD, Lipase MER "AMANO", Lipase G "AMANO" 50, Lipase DF "AMANO" 15, DSM Panamoagolden, Panamoaspring, Pikantase R800, Pikantase A, Bakezyme L80.000B, Cakezyme, Makisapearl A2 , Paratase 20000L by Novozymes Japan, Lipopan 50BG, Lipase SL by Nagase Sangyo Co., Ltd., Lipase MY, Lipase OF, Lipase QLM, Lipase TL, Lipase PL, Phospholipase D, Lilipase A by Nagase ChemteX -10D, Lilipase AF-5, Denabake RICH, PLA2 Nagase 10P/R, PLA2 Nagase L/R, Phospholipase A1 manufactured by Mitsubishi Chemical Foods, LPL-311 and LPL-314 manufactured by Toyobo Corporation.

In addition, esterases (including phosphatase) include Sumizyme PHY manufactured by Nippon Chemical Industries, Ltd., phytase manufactured by DSM Corporation, Sumizyme PM manufactured by Nippon Chemical Industries Corporation, and nuclease "AMANO" manufactured by Amano Enzyme Corporation. G. OPTIMASE AE manufactured by Danisco Japan, LPP-209, COE-301, COE-302, COE-311, COE-313 manufactured by Toyobo Corporation.

In addition, as a substance containing both peptidase and lipase, Tecanose of Tressbio Research Institute is mentioned. The hydrolase may contain hydrolase other than lipase and protease. For example, glucoamylase, which breaks down starch, may be included.

The hydrolytic enzyme is preferably contained in the antimicrobial agent in the form of an aqueous solution at about 0.1 to 1.5% by mass. When it is less than 0.1 mass %, the desired antibacterial effect is not easily obtained, and when it contains more than 1.5 mass %, the antibacterial effect is saturated. Preferably it is 0.5-1.0 mass %.

(pH adjuster) The antibacterial agent containing plant-derived antibacterial components and hydrolytic enzymes is preferably used in the form of an aqueous solution. The pH of the aqueous solution is 6.5 to 10, more preferably 7 to 9, and still more preferably about 7 to 8. When the aqueous solution is weakly alkaline, a good antibacterial effect can be obtained. In order to adjust the pH of the aqueous solution, it is preferable to contain a pH adjuster. The pH adjuster can be, for example, sodium bicarbonate, so-called baking soda. Sodium bicarbonate is a component with high safety to the human body, and it is preferable that the pH of the aqueous solution can be easily adjusted to about 7 to 8. In addition, the pH adjuster is not limited to sodium bicarbonate, and a material that can make the pH of the aqueous solution weakly alkaline can be appropriately selected. The blending amount of the pH adjuster can be added in an amount that can adjust the pH of the aqueous solution to the above-mentioned suitable range according to the raw material of the pH adjuster.

(essential oil) The antibacterial agent may further contain essential oils in addition to the extracts extracted from plants as described above. Essential oils refer to volatile oils obtained from plants. Essential oils with antibacterial and antiviral effects among essential oils can be used as antibacterial agents. Examples of essential oils having antibacterial and antiviral effects include cypress essential oil, cypress essential oil, peppermint essential oil (mint oil), eucalyptus essential oil, ravensara essential oil, lavender essential oil, lemon essential oil, and rosemary essential oil. It can be extracted from these raw materials to produce essential oils, or commercially available essential oils can be obtained and used in antibacterial agents. The blending ratio of the essential oil in the antibacterial agent in the form of an aqueous solution is not particularly limited, but it is preferably about 0.1 to 1.0 mass %.

(Antibacterial ingredients other than plant-derived antibacterial ingredients) The antibacterial agent may further contain antibacterial components other than plant-derived antibacterial components. The antibacterial effect can be further enhanced by containing antibacterial ingredients other than plant-derived antibacterial ingredients. Examples of antibacterial components other than plant-derived antibacterial components include copper, silver, cetylpyridinium chloride (CPC), isopropylmethylphenol (IPMP), sodium lauryl sarcosinate (LSS), chlorine hydrochloride Hexidine (CHX) or triclosan (TC), etc. The blending ratio of the antibacterial components other than the plant-derived antibacterial component in the antibacterial agent in the form of an aqueous solution is not particularly limited, but it is preferably about 0.01 to 0.2 mass %.

(cellulose derivative) The antibacterial agent may further contain a cellulose derivative. Sustainability can be improved by containing cellulose derivatives. Cellulose derivatives are cellulose or cellulose substituted or chemically modified compounds or salts thereof. The degree of substitution of cellulose is not particularly limited. The cellulose derivative includes, for example, cellulose, methyl cellulose, carboxymethyl cellulose, and sodium carboxymethyl cellulose, preferably carboxymethyl cellulose or a salt thereof (eg, sodium carboxymethyl cellulose). Sodium carboxymethyl cellulose can be used in the form of a thickener or the like as a commercial item. There are food additive grades and industrial grades, and either can be used depending on the application of the dispersion.

The particle size of the powder of the cellulose derivative is not particularly limited, and a commercially available cellulose derivative can be used as it is.

The content of sodium carboxymethyl cellulose is 2 mass % or less in the antibacterial agent in the form of an aqueous solution, preferably 0.1 to 2 mass %.

(hydroxyapatite) The antibacterial agent may further contain hydroxyapatite. Hydroxyapatite is porous and can improve the antibacterial effect by adsorbing pathogenic bacteria or viruses. As the hydroxyapatite, commercially available hydroxyapatite can be used. Commercially available hydroxyapatite has mineral origin or biological origin; any hydroxyapatite can be used for mineral origin or biological origin, preferably biological origin hydroxyapatite. The biologically derived hydroxyapatite refers to hydroxyapatite obtained by using biological constituents (skeleton, coral, shell, eggshell, etc.) as a calcium source for obtaining hydroxyapatite. Hydroxyapatite obtained from eggshells has the trade name BIOAPATITE (trademark) of BioApatite Co., Ltd. The amount of hydroxyapatite is preferably 1 mass % or more, more preferably 5 mass % or more, and even more preferably 10 mass % or more and 30 mass % or less in the antibacterial agent in the form of an aqueous solution.

(other ingredients) Antibacterial agents may contain other ingredients as required.

For example, an antibacterial agent with a suitable composition includes an antibacterial agent derived from a plant containing mangosteen extract and a hydrolytic enzyme containing protease, or an antibacterial agent derived from a plant containing peppermint oil and xylitol and the hydrolytic enzyme containing protease. Antibacterial agents. In the antibacterial agent in which the plant-derived antibacterial component contains peppermint oil and xylitol, and the hydrolytic enzyme contains protease, the suitable blending ratio of peppermint oil and xylitol is 0.3 to 0.5 mass % of peppermint oil and 0.1 to 0.1 mass % of xylitol. 5.0% by mass.

(Manufacturing method) The manufacturing method of an antibacterial agent is demonstrated with respect to the case where the antibacterial component derived from a plant and a hydrolysis enzyme are included as basic components. Antibacterial agents are produced by adding plant-derived antibacterial ingredients and hydrolytic enzymes to water. At this time, the pH of the water is preferably pH 6.5 to 10, more preferably 7 to 9, and still more preferably 7 to 8. In order to adjust the pH of the water to pH 6.5 to 10, for example, a pH adjuster such as sodium bicarbonate is added to the water. The order of adding to the water should be sodium bicarbonate first and plant-derived antibacterial ingredients and hydrolytic enzymes last. However, sodium bicarbonate, plant-derived antibacterial ingredients and hydrolytic enzymes can also be added at the same time, or the source can be added first. After the plant's antibacterial ingredients and hydrolytic enzymes, sodium bicarbonate is added quickly. High-purity water such as reverse osmosis membrane water can be used for water, and tap water can also be used depending on the application. According to the findings of the inventors of the present case, if the antibacterial ingredients derived from plants such as mangosteen extract, ginger extract, and wasabi extract are mixed with hydrolytic enzymes, emulsification will occur, resulting in the problem of liquid becoming cloudy. By mixing each component so that pH becomes the said range, cloudiness and emulsification can be suppressed, and a transparent antibacterial agent can be obtained.

(use) The antibacterial agent can be stored in a container and applied to the object to be sterilized by spraying; it can also be wiped with a non-woven cloth or cloth impregnated with the antibacterial agent. Example

Hereinafter, the content of the present case will be described in more detail based on examples. The scope of this case should not be limited by the examples.

(specimen) Samples of the examples of the antibacterial agent, samples of sterilized physiological saline as Comparative Example 1, and samples of Comparative Example 2 containing antibacterial components derived from plants and not containing hydrolytic enzymes were prepared. In the detection system of the embodiment, the plant-derived antibacterial component contains 0.1% by mass of mangosteen extract, and the hydrolytic enzyme contains 0.1% by mass of protease. It was prepared by adding sodium bicarbonate to pure water to make pH 7.8, and adding the above-mentioned mangosteen extract and protease. The inspection system of Comparative Example 2 contained 0.1% by mass of the mangosteen extract as the plant-derived antibacterial component. It is prepared by adding the above mangosteen extract to pure water.

(experiment method) (1) Test bacteria The following five test bacteria were prepared. Escherichia coli NBRC3972 (Escherichia coli) Staphylococcus aureus NBRC13276 (Staphylococcus aureus) Streptococcus mutans NBRC13955 (tooth decay bacteria) Porphyromonas gingivalis JCM12257 (periodontal bacteria) Candida albicans NBRC1594 (Candida)

(2) Preparation of test bacterial solution Escherichia coli (hereinafter referred to as "E. coli") and Staphylococcus aureus (hereinafter referred to as "S. aureus") were pre-cultured on SOD agar medium at 30°C for 24 hours. The precultured bacteria were suspended in sterilized physiological saline to prepare a test bacterial solution at about 10 ⁸ cells/mL. Streptococcus mutans (hereinafter referred to as "S. mutans") was pre-cultured (anaerobic culture) at 35°C for 24 hours on GAM agar medium for the test bacteria. The precultured bacteria were suspended in sterilized physiological saline to prepare a test bacterial solution at about 10 ⁸ cells/mL.

Porphyromonas gingivalis (hereinafter referred to as "P. gingivalis") was pre-cultured (anaerobic anaerobic) on GAM agar medium supplemented with hemin-menadione (hereinafter referred to as "H/M+") at 35°C for 48 hours. nourish). The previous pre-culture was inoculated into H/M+GAM nutrient liquid medium, and pre-culture was performed at 35° C. for 48 hours (anaerobic culture). The pre-culture liquid was diluted with H/M+GAM nutrient liquid medium, and it adjusted to about 10 ⁸ cells/mL to prepare a test bacterial liquid. In addition, the addition of hemin-menaquinone is obtained by adding 1 mL of hemin solution and menaquinone solution to 100 mL of culture medium, respectively. This hemin solution is obtained by dissolving 50 mg of hemin in 1 mL of 1N NaOH, Add pure water to make 100mL; Menaquinone solution is made by dissolving 5 mg of menaquinone in 1mL of ethanol, and adding pure water to make 100mL.

Candida albicans (hereinafter referred to as "C. albicans") was pre-cultured on potato dextrose agar medium at 25°C for 48 hours, and the pre-cultured bacteria were suspended in sterilized physiological saline to prepare about 10 ⁸ cells/mL And make the test bacteria liquid.

(3) Test operation Take 19.8 g of the specimen of the embodiment in a sterilized vial, and inoculate a 1% amount (0.2 mL) of the test bacterial solution. It was stored under the action conditions described below, and 1 g of it was taken after a predetermined time and diluted with 9 mL of diluent. This dilution was further serially diluted, and the number of bacteria was determined by the agar plate pouring method. In addition, as for the dilution, LP dilution was used for E. coli, S. aureus, S. mutans, and C. albicans, and H/M+GAM nutrient liquid medium was used for P. gingivalis. The LP diluent is 1 g of polyprotein, 7 g of egg lecithin, 20 g of polysorbate 80, and 980 mL of pure water. In addition, the samples of Comparative Example 1 and Comparative Example 2 were similarly operated, and the number of viable bacteria was measured immediately after the inoculation and after the final action time.

(4) Action conditions Action temperature: 25°C (thermostat) Action time: 10 minutes for the specimen Just 10 minutes after the control inoculation (5) Determination medium and culture conditions for the number of living bacteria E. coli and S. aureus were cultured on SCDLP agar medium at 30°C for 3 days. S. mutans were cultured on GAM agar medium at 35°C for 3 days (anaerobic culture). The P. gingivalis line was cultured on H/M+GAM agar medium at 35°C for 3 to 5 days (anaerobic culture). The C. albicans line was cultured on GPLP agar medium at 25°C for 3 days.

(test results) The test results are shown in Table 1.

(total) According to Table 1, if the sterilization efficiency of the samples of the embodiment is expressed by the sterilization rate within 10 minutes, it is as follows: E. Coli: 99.99% S. aureus: 99.99% S. mutans: 99.99% P. gingivalis: 99.99% C. albicans: 99.99% It can be seen from the above tests that the antibacterial agents of the examples have excellent bactericidal effect. Moreover, in the Example containing protease, the antibacterial effect on Escherichia coli and Staphylococcus aureus was improved compared with Comparative Example 2 which did not contain protease.

Next, two petri dishes containing the above-mentioned SOD agar medium were prepared. The agar culture system of each petri dish is divided into two areas. One area is to observe the attachment and reproduction of planktonic bacteria in ordinary households. The stick was wiped on the agar medium to observe the attachment and reproduction of saprophytic bacteria. One of the agar mediums is coated with the sterilizing liquid of the embodiment, and the other agar medium is coated with 80% ethanol disinfectant. Observe the agar medium after standing for 12 hours. A photograph showing the surface of the agar medium is shown in FIG. 1 . In Figure 1, the left side of each petri dish is the observation area for planktonic bacteria, and the right side of the diagram is the observation area for saprophytic bacteria in the contaminated site. The agar medium in the lower left of the figure is a sample coated with the sterilizing solution of the embodiment, and the agar medium in the lower right of the figure is a sample coated with an 80% ethanol disinfectant. Fig. 2 is a hand-drawn diagram showing the propagation state of cells on each agar medium. As can be seen from Figure 1 and Figure 2, the samples coated with 80% ethanol disinfectant were observed to multiply by planktonic bacteria 1 and saprophytic bacteria 2; on the other hand, the samples coated with the sterilization solution of the embodiment were not observed. Planktonic bacteria multiply, and as a result, the sterilizing solution of the example is more durable than the 80% ethanol sanitizing solution.

Next, three test pieces composed of photosensitive photographic paper are prepared. In each test piece, one piece of the test piece is dripped with the sterilizing solution of the embodiment to the front part, another test piece is dripped with 80% ethanol disinfectant to the front end part, and another test piece is added dropwise to the front end part. Household sodium hypochlorite bleach (dilute 30mL of bleach in 5L of water). The surface properties of the test pieces before dropping are shown in FIG. 3 , and the surface properties of the test pieces after immersion for 30 minutes are shown in FIG. 4 . In Fig. 3 and Fig. 4, the test piece of No. 1 is dripped with the sterilizing liquid of the embodiment, the test piece of No. 2 is dripped with 80% ethanol disinfectant, and the test piece of No. 3 is dripped with dipping solution Use sodium hypochlorite bleach. According to Fig. 4, the test piece dripped with the sterilization liquid of the embodiment, wherein the part with the sterilization liquid dripped into it is discolored, it is judged that this is because the sterilization liquid of the embodiment removes the gelatin on the surface of the photographic paper by the action of protease. caused by decomposition. It was confirmed that the protein was decomposed by the action of the hydrolytic enzyme in the sterilization solution, and it can be speculated that the antibacterial agent of the Example can enhance the sterilization effect. The test piece immersed in 80% ethanol disinfectant and the test piece immersed in household sodium hypochlorite bleach did not change color, and the gelatin was not decomposed.

Next, as an antibacterial agent, the bactericidal effect was examined with respect to another example (Example 2) whose composition was different from that of the above-mentioned examples.

(specimen) The detection system of the Example contains 0.3 mass % of peppermint oil which is a plant-derived antibacterial ingredient and mainly contains menthol, 0.3 mass % of xylitol, and 0.1 mass % of protease as a hydrolytic enzyme. After adding sodium bicarbonate to pure water to adjust pH to 7.8, the above-mentioned peppermint oil, xylitol and protease were added and prepared.

(experiment method) (1) Test bacteria The following five test bacteria were prepared. Escherichia coli NBRC3972 (Escherichia coli) Pseudomonas aeruginosa BRC13275 (Pseudomonas aeruginosa) Staphylococcus aureus NBRC13276 (Staphylococcus aureus) Candida albicans NBRC1594 (Candida) Trichophyton mentagrophytes NBRC6124 (Trichophyton)

(2) Preparation of test bacterial solution Escherichia coli (hereinafter referred to as "E. coli"), Staphylococcus aureus (hereinafter referred to as "S. aureus") and Candida albicans (hereinafter referred to as "C. albicans") were prepared in the same way as the test bacterial solutions of the previous examples , Pseudomonas aeruginosa (hereinafter referred to as "P. aeruginosa") is the same as E. coli and S. aure.

Trichophyton mentagrophytes (hereinafter referred to as "T. mentagrophytes") is a pre-culture of the test bacteria on Saprox dextrose agar medium at 25°C for 10 to 14 days. Add 0.05% of sorbitol ester 80 to sterile physiological saline, and pulverize it with a homogenizer. This solution was filtered with sterilized gauze folded into quarters, and then adjusted to about 10 ⁷ cells/mL to prepare a test bacterial solution.

(3) Test operation Take 19.8 g of the specimen of Example 2 in a sterilized vial, and inoculate a 1% amount (0.2 mL) of the test bacterial solution. It was kept under the same working conditions as in the previously described examples, the same amount was taken after the same prescribed time, and diluted with the same amount of the same diluent. This dilution was further serially diluted, and the number of bacteria was determined by the agar plate pouring method. In addition, the culture medium and culture conditions for the determination of the number of viable bacteria are as follows: E. coli, P. aeruginosa, and S. aureus were used SCDLP agar medium, 30℃, 3 days. The C. albicans line was cultured on GPLP agar medium at 25°C for 3 days. T. mentagrophytes was harvested on Sapro's Dextrose LP agar medium at 25°C for 10 to 14 days.

As far as (test results), in E. coli (Escherichia coli), P. aeruginosa (Pseudomonas aeruginosa), S. aureus (Staphylococcus aureus), C. albicans (Candida), T. mentagrophytes (Trichophytes) any one In this case, the bacterial value after 10 minutes did not reach 10. If the sterilization efficiency of the specimens of the Example was expressed by the sterilization rate within 10 minutes, any bacterial species was 99.99%.

Fig. 1 is a photograph showing the surface properties of the agar medium in which the growth of planktonic bacteria and saprophytic bacteria was observed. [Fig. 2] A hand drawing of the agar medium of Fig. 1. [Fig. Fig. 3 is a photograph showing the surface properties of the test piece before being immersed in the antibacterial agent, alcohol solution, and household sodium hypochlorite of Examples. Fig. 4 is a photograph showing the surface properties of the test piece immersed in the antibacterial agent, alcohol solution, and household sodium hypochlorite of Examples.

Claims (10)

An antibacterial agent, which contains plant-derived antibacterial components and hydrolytic enzymes. The antibacterial agent of claim 1, wherein the aforementioned plant-derived antibacterial ingredient comprises at least one extract selected from the group consisting of mangosteen extract, horseradish extract and ginger extract. The antibacterial agent of claim 1 or 2, wherein the hydrolytic enzyme comprises at least one enzyme selected from the group consisting of lipolytic enzymes and proteolytic enzymes. The antibacterial agent according to any one of claims 1 to 3, further comprising a pH adjuster. The antibacterial agent according to any one of claims 1 to 4, further comprising an essential oil. The antibacterial agent according to any one of claims 1 to 5, further comprising an antibacterial component other than the aforementioned plant-derived antibacterial component. The antibacterial agent according to any one of claims 1 to 6, further comprising a cellulose derivative. The antibacterial agent according to any one of claims 1 to 7, which is a transparent liquid. A method for producing an antibacterial agent, comprising the steps of adding a plant-derived antibacterial component and a hydrolytic enzyme to water or an aqueous solution of pH 6.5 to 10. An antibacterial agent comprising menthol, xylitol and hydrolase.

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