TW202128028A - Food composition comprising low-glycosides ginsenoside using hydroponically grown ginseng leaf and preparation method thereof - Google Patents

Abstract

The invention relates to a food composition containing low-glycosides ginsenoside using hydroponically grown ginseng leaves, and, more particularly, to a method including a step of steaming hydroponically grown ginseng leaves for preparing a ginseng leaf concentrated solution with increased low-glycosides ginsenoside content, a ginseng leaf concentrated solution and a food composition prepared by the method. Moreover, the preparation method includes the steps of: performing steaming process for dehydrated and hydroponically grown ginseng leaves, performing drying and pulverizing for the steamed ginseng leaves, and extracting the dried and pulverized ginseng leaves for concentration.

Description

Food composition containing low-glycosides ginsenoside using hydroponic cultivated ginseng leaves and preparation method thereof

The present invention relates to a food composition containing low-glycosides ginsenosides using hydroponic cultivated ginseng leaves. More specifically, it relates to the preparation of low-glycosides ginsenosides with increased content of ginseng by including a step of steaming the hydroponic cultivated ginseng leaves Method for leaf concentrate, ginseng leaf concentrate and food composition prepared thereby.

Ginseng (Panax ginseng C. A. Meyer) is a very high-performance medicinal material and is actively cultivated in Korea. In the past few decades, with the development of farming techniques such as the application of fertilizers and pesticides, the production of ginseng has been increasing. In terms of business, the root of ginseng is considered to be the main transaction part, while ginseng leaves are often discarded during the harvesting process because they are considered to be by-products.

Ginsenoside (Ginsenoside) is a triterpene (Triterpene) saponin in ginseng. Saponins are chemically a kind of glycoside (Glycoside) compounds. They exist in the roots, stems, leaves, skins, seeds, etc. of ginseng. They were previously considered non-nutritive However, recently it has shown anti-cancer, anti-oxidation, and cholesterol-lowering effects, and has begun to attract attention as a physiologically active substance.

In Korean medicine, it has been used as a cardiotonic or diuretic. In particular, among the various active ingredients of ginseng, saponins play a major pharmacological role. Ginseng saponins have a unique chemical structure and are different from the saponins found in other plants. They also have unique pharmacological effects. Therefore, as the meaning of Ginseng glycoside (Glycoside), it is called "Ginsenoside".

Recently, various studies have been conducted and found that ginseng leaves also contain a variety of ginsenosides, and in order to use the ginsenoside extract present in the leaves, a comparative study is being conducted with the root ginsenosides.

Specific ginsenosides are also purified from discarded leaves, so economic products for health foods and even pharmaceuticals can be expected. Ginsenosides can be divided into triterpene type and oleanolic acid type such as Protopanaxadiol (PPD) and even Protopanaxatriol (Protopanaxatriol, PPT) aglycon form. Ginsenosides that form aglycone containing glycosides can be converted into other forms of ginsenosides by the release of glycosides. This conversion can be caused by heat, acid decomposition, and enzyme treatment.

It is known that the crude saponin content of ginseng leaves is 19.58%, which is higher than that of stems (2.38%) or roots (4.78%), and the content of ginsenosides of ginseng leaves is 10.84%, which is higher than that of stems (0.64%) and roots. (2.37%), the content is higher (Korean J. Ginseng Sci. 11(2). 118-112, 1987). In addition, there are many forms of ginsenosides, and they show different effects, so they have been the main goal of research. Rb1: Participate in embryonic development, DNA of bone marrow cells (RNA-lipid synthesis, promotion), and affect the reproductive system by promoting the production of sex hormones Rc: Analgesic effect, promote protein and lipid synthesis Rb2: Anti-diabetic effect, immune regulation effect, cholesterol metabolism Rb3: physiological activity Rd: Promote the secretion of adrenal cortex hormones Rg3: Inhibition of cancer cell metastasis, inhibition of platelet aggregation, and inhibition of anticancer drug resistance Rh2: Inhibit cancer cell proliferation, inhibit tumor proliferation, skin immune effect

According to many studies reported so far, it is known that in the process of processing and using plants, with drying conditions, inherent functional substances are transformed into other substances or the dissolution content changes during dissolution. Research on the analysis of ginsenosides in ginseng leaves is far less active than roots. In particular, there are not many examples of studies on the effects of processing such as heat treatment. Therefore, it is necessary to try to use the useful ingredients of ginseng leaves. So far, the truth is that there is no traditional technique that uses steaming to change the functionality of herbs such as ginseng leaves.

In this context, the inventors of the present invention have conducted continuous research to increase the content of low-glycosides ginsenosides, confirming that when hydroponic cultivated ginseng leaves are used for the steaming process, the content of low-glycosides ginsenosides increases and can be prepared to contain Its ginseng leaf concentrate has completed the present invention.

[Prior Art Literature]

[Patent Literature] (Patent Document 1) Korean granted patent 10-0325778

Technical issues

An object of the present invention is to provide a method for preparing a ginseng leaf concentrate with increased content of 1-glycoside ginsenoside, which includes the step of steaming hydroponic ginsenoside leaves from which water has been removed; The step of drying and crushing the steamed ginseng leaves; and the step of extracting and concentrating the dried and crushed ginseng leaves.

Another object of the present invention is to provide a method for increasing the content of 1-glycoside ginsenoside when preparing ginseng leaf concentrate, including the step of steaming the hydroponic ginseng leaves from which water has been removed; and the above-mentioned steamed ginseng leaves A step of drying and crushing; and a step of extracting and concentrating the dried and crushed ginseng leaves.

Another object of the present invention is to provide a ginseng leaf concentrate prepared by the above preparation method.

Problem solving methods

The specific description is as follows. On the other hand, the descriptions and embodiments disclosed in the present invention can also be applied to other descriptions and embodiments. That is, all combinations of various elements disclosed in the present invention belong to the scope of the present invention. In addition, it is not considered that the scope of the present invention is limited by the specific description described below.

As one aspect of achieving the above-mentioned object, a method for preparing a ginseng leaf concentrate with increased content of 1-glycoside ginsenoside is provided. The step of drying and crushing ginseng leaves and the step of extracting and concentrating the dried and crushed ginseng leaves.

As another aspect of achieving the above objective, there is provided a method for increasing the content of 1-glycoside ginsenoside when preparing ginseng leaf concentrate, which includes the step of steaming the hydroponic ginseng leaves from which water has been removed, and the method of steaming the above-mentioned steaming treatment. The step of drying and crushing ginseng leaves and the step of extracting and concentrating the dried and crushed ginseng leaves.

As another aspect of achieving the above-mentioned object, the present invention provides a ginseng leaf concentrate prepared by the above-mentioned preparation method.

Specifically, the present invention provides a ginseng leaf concentrate having an increased content of low-glycoside ginsenoside (1-glycoside ginsenoside), and the ginseng leaf concentrate can be provided as a food composition for skin whitening and dark spot improvement.

As a medicinal plant that regenerates itself only in the Far East of Asia, ginseng is a kind of excellent strong effect, and contains medicinal ingredients called saponin, and has anti-cancer, anti-oxidation, whitening, cholesterol-lowering effects and has a variety of diseases. Plants with therapeutic effects. Generally, ginseng is cultivated in the open field for more than 4 years, and the 4-year-old root and the 6-year-old root are mostly used. However, this open field cultivation method has the following disadvantages: it takes a long time and has space limitations. Therefore, the present invention uses a method of cultivating ginseng in a culture solution made of water and water-soluble nutrients, that is, hydroponic cultivation.

Ginseng cultivated by hydroponic cultivation has no harmful substances such as pesticides, so not only the root of ginseng can be used, but also all parts such as leaves and stems can be used. In addition, hydroponic cultivated ginseng leaves can achieve a higher ginsenoside content than ginseng leaves cultivated in the open field.

Ginsenoside refers to the saponins contained in ginseng. Saponins are derived from the Latin word Sapo (soap), which is a natural surface active substance that produces a lasting foam after being shaken in water. It is a general term for triterpenoids and steroid glycosides widely distributed in the plant kingdom. It has a chemical structure similar to cholesterol, so it inhibits the absorption of cholesterol and also helps excrete it. If you eat meat and other animal foods for a long time, the body will produce lipid peroxides similar to oxidized fat, which is one of the carcinogenic factors. Ginsenosides can decompose lipid peroxides, cut off the root cause of cancer, prevent free radicals from destroying cells and inhibit mutations, thereby reducing the incidence of colon cancer in particular. In addition, reducing the energy accumulated in body fat is one of its inherent effects. The results of animal experiments even have the function of fundamentally improving obesity.

In addition, in order to ingest the medicinal components of ginseng, it is usually processed into the form of medicines or health foods, but existing ginseng drinks and foods only use ginseng roots, and hardly use ginseng leaves and stems. However, since ginseng leaves and stems contain a large amount of ginsenoside components, the present invention uses ginseng leaves.

In addition, the ginseng leaves used in the present invention are characterized in that they can be Panax ginseng or Panax quinquefolius, but are not limited thereto.

In the present invention, the above-mentioned hydroponic cultivated ginseng leaves may preferably be 1 to 3 years old.

The term "glycoside" in the present invention, as one of the widely distributed components in the plant kingdom, means that it is decomposed into sugar moieties and non-glycosides called aglycons or ligands under the action of acids, bases or appropriate hydrolytic enzymes. The general term for substances in the sugar part. When ginsenosides are absorbed by the body, glycosides are released and absorbed as low-glycosides, and by increasing low-glycosides, the absorption rate in the body can be increased.

In the present invention, the above-mentioned steaming refers to aging by steaming. Specifically, in the present invention, a steaming treatment step may be performed to increase the content of the required low-glycoside ginsenoside. In the present invention, the step of the above-mentioned steaming treatment is not limited to this, but can be carried out for more than 4 hours, specifically, it can be carried out preferably for 4 hours to 6 hours, and more specifically, it can be carried out for 5 hours.

If the above-mentioned steaming treatment step is less than 4 hours, the effect of increasing the ginsenoside content may be slightly less.

In addition, the step of the above-mentioned steaming treatment is not limited to this, but can be carried out at 90°C to 110°C, specifically, it can be carried out at 95°C to 105°C. In an embodiment of the present invention, the steaming treatment is carried out at 98°C.

In the present invention, the above-mentioned drying step is not limited to this, but the moisture content of the above-mentioned ginseng leaves may be between 1% and 15% by drying at 50°C to 70°C for 10 hours to 100 hours.

Specifically, it can be dried in a drying oven at 60°C for 12 hours until the moisture content of the ginseng leaves reaches 15%.

The drying time can be changed according to the drying temperature and other conditions, and preferably, it can be dried until the moisture of the ginseng leaf is 15% or less.

In the present invention, the above-mentioned extraction and concentration step is not limited to this, but it may preferably be extracted by adding alcohol.

The concentration of the above alcohol is not limited to this, but may be 60% to 80%.

The above extraction step is not limited to this, but it may preferably be extracted for 7 hours to 9 hours.

The step of concentration under reduced pressure is not limited to this, but it can be concentrated to 65% to 90% Brix.

The aforementioned low-glycoside ginsenosides are not limited thereto, but may be 1-glycoside ginsenosides, specifically, those including ginsenoside F1, compound K, Rh2, Rh3, and/or Rk2.

Compared with dried red ginseng, red ginseng concentrate, dried ginseng leaves, dried ginseng stems, steamed red ginseng, and steamed ginseng stems, the ginseng leaf concentrate prepared by the preparation method of the present invention can achieve low glycoside Increase in ginsenoside content.

The ginseng leaf concentrate of the present invention suppresses skin darkening by increasing the content of low-glycoside ginsenosides, and can be provided as a food composition for skin whitening and dark spot improvement.

The above food composition may not only contain the ginseng leaf concentrate of the present invention, but also various nutrients, vitamins, electrolytes, flavors, colorants, sweeteners, sour agents, pectic acid and its salts, alginic acid and its Salts, organic acids, protective colloid thickeners, pH adjusters, stabilizers, preservatives, glycerin, carbonating agents for alcoholic carbonated beverages, etc.

The above-mentioned food composition contains all ingredients including ginseng leaf concentrate, preferably, not exceeding the food guidelines (South Korea regulations), food additives guidelines (South Korea regulations), health functional food guidelines (South Korea regulations), China The maximum use value stipulated by the sanitary regulations (Chinese regulations), etc.

Invention effect

The invention can provide a ginseng leaf concentrate with an increased content of low-glycosides and ginsenosides. In addition, the ginseng leaf concentrate of the present invention in which hydroponic ginseng leaves are subjected to a steaming process can be provided as a food composition for skin whitening and dark spot improvement.

Hereinafter, the present invention will be explained in more detail through examples. These examples are intended to explain the present invention more specifically, and the scope of the present invention is not limited to these examples.

Preparation example: Preparation of hydroponic cultivated ginseng leaf concentrate

Choose by collecting 1-3 year-old ginseng leaves from hydroponic cultivation, and prepare fresh green leaves. Wash the ginseng leaves and remove the water, and then steam them in a sterile autoclave at 98°C for 5 hours. After the steaming treatment, it is dried in a drying cabinet at 60°C for more than 12 hours so that the water content of the ginseng leaves is 15% or less. The dried ginseng leaves were crushed using a blender and stored at room temperature. Put in 70% alcohol (alcohol) 8 times, and circulate the crushed ginseng leaves for 8 hours, and then concentrate under reduced pressure to Brix above 65% to obtain a concentrated liquid.

The same method is also applied to red ginseng and ginseng stems to prepare a concentrate.

Example 1: Analyze the content of 1-glycoside ginsenoside in ginseng leaves that have undergone a steaming process

By thermal, enzymatic or acid decomposition, ginsenosides are converted from the form with 3-4 sugars attached to the form where 1-4 sugars are released. In the present invention, the 1-glycoside ginsenoside (F1, compound K, Rh2, Rh3, Rk2) is analyzed to compare the changes in the ginsenoside content before and after the steaming treatment as described above.

For the ginsenoside content, the sample was dissolved in 70% methanol and analyzed by the LC-MS/MS system (Waters TQ-D system, USA).

Example 1-1: Analysis of ginsenoside content before steaming

First, it was confirmed how much the 1-glycoside ginsenoside content of red ginseng and various forms of ginseng has not been steamed. Specifically, before steaming dried red ginseng, the commercially available Zhengguanzhuang red ginseng concentrate 1, the commercially available Fengji ginseng red ginseng concentrate 2, dried ginseng leaves or dried ginseng stems, the dried pulverized matter is the object The content of 1-glycoside ginsenoside was measured.

[Table 1] (mg/g) Dried red ginseng Red ginseng concentrate 1 Red Ginseng Concentrate 2 Dried ginseng leaves Dried ginseng stems F1 0.000 0.005 0.017 0.983 0.192 Compound K 0.000 0.002 0.004 0.004 0.000 Rh2 0.000 0.006 0.030 0.099 0.001 Rh3 0.000 0.002 0.006 0.000 0.000 Rk2 0.000 0.002 0.006 0.000 0.000

As shown in Table 1 above, it can be confirmed that the 1-glycoside ginsenoside of the present invention in the dried red ginseng before steaming and the commercially available red ginseng concentrates 1 to 2, compared with the ginseng leaves and dried ginseng stems before steaming The content of (1-glycoside ginsenoside) F1, compound K, Rh2, Rh3 or Rk2 is low. In addition, it can be confirmed that the content of ginsenoside F1 in dried ginseng leaves is relatively high, 0.983 mg/g, but overall, the content of 1-glycoside ginsenoside is low before steaming.

Example 1-2: Analysis of ginsenoside content after steaming treatment

As described above, it was confirmed that the content of 1-glycoside ginsenoside was not significant before the steaming process, and the ginsenoside content after the steaming process of various forms of red ginseng, ginseng leaves and ginseng stems was analyzed. Specifically, the content of 1-glycoside ginsenoside was measured by steaming red ginseng 2 to 9 times, steaming ginseng leaves 1 to 2 times, and steaming ginseng stems 1 to 2 times.

[Table 2] (mg/g) Steamed red ginseng twice Steamed red ginseng 3 times 5 steamed red ginseng 7 steamed red ginseng 9 steamed red ginseng 1 steamed ginseng leaf 2 steamed ginseng leaves 1 steamed ginseng stem 2 steamed ginseng stems F1 0.010 0.010 0.010 0.000 0.000 1.527 1.421 0.461 0.723 Compound K 0.000 0.000 0.000 0.010 0.010 0.103 0.187 0.013 0.022 Rh2 0.000 0.000 0.020 0.040 0.140 2.087 2.757 0.022 0.057 Rh3 0.000 0.000 0.030 0.050 0.110 0.526 0.964 0.016 0.031 Rk2 0.000 0.000 0.030 0.040 0.130 0.656 1.062 0.021 0.051

As shown in Table 2 above, it was confirmed that even if red ginseng is steamed several times and ginseng stem is steamed several times, the content of 1-glycoside ginsenoside (1-glycoside ginsenoside) F1, compound K, Rh2, Rh3 or Rk2 is not Will increase substantially. On the contrary, according to the comparison of Table 1 and Table 2 above, for the steamed ginseng leaves, compared with the unsteamed ginseng leaves, 1-glycoside ginsenoside (1-glycoside ginsenoside) F1, compound K, Rh2 The content of, Rh3 or Rk2 increased significantly.

Example 2: Analysis of 1-glycoside ginsenoside content in ginseng leaves of different cultivation methods

As mentioned above, it is confirmed that even if the steaming process is carried out, there will be no special increase in red ginseng or ginseng stems, but the content of 1-glycoside ginsenoside in ginseng leaves is significantly increased. Cultivation method to measure ginsenoside content.

Specifically, hydroponic cultivated ginseng leaves and open field cultivated ginseng leaves were used as objects, and the same steaming treatment as the above-mentioned preparation example was performed to measure the ginsenoside content.

As shown in Table 3 below, hydroponic cultivation of 1 to 3 years old ginseng leaves and open field cultivation of ginseng leaves were steamed and treated at 98°C for 5 hours to measure 1-glycoside ginsenoside F1 and compound K , Rh2, Rh3 or Rk2 content.

[table 3] (mg/g) Hydroponic cultivation of ginseng leaves Cultivated ginseng leaves in the open field F1 1.527 1.051 Compound K 0.103 0.055 Rh2 2.087 1.357 Rh3 0.526 0.328 Rk2 0.656 0.415

As shown in Table 3 above, it can be confirmed that the content of 1-glycoside ginsenoside in hydroponic ginseng leaves is higher than that in ginseng leaves cultivated in the open field. Therefore, it can be confirmed that the use of hydroponic ginseng leaves can increase the 1-glycoside ginsenoside content compared to open field cultivation.

Example 3: Analyze the ginsenoside content of ginseng leaves according to the steaming treatment

In order to analyze the content of other glycosides in the ginsenosides according to the steaming treatment, the aim was to determine the content of other glycosides other than 1 glycoside in dry ginseng leaves without steaming treatment and ginseng leaves after steaming treatment. Specifically, measure the low glycoside ginsenoside (1-glycosides ginsenoside) F1, compound K, Rh2, Rh3, Rk2, or ginsenosides other than low glycosides in dried ginseng leaves and ginseng leaves that have been steamed for 1 to 2 times The contents of Rb1, Re, Rg1, F2, and Rg3 are shown in Table 4 below.

[Table 4] (mg/g) Dried ginseng leaves 1 steamed ginseng leaf 2 steamed ginseng leaves Rb1 0.0586 0.257 0.206 Re 15.087 6.776 5.237 Rg1 0.569 2.376 1.542 F2 8.973 8.698 6.156 Rg3 0.014 0.742 1.017 F1 0.983 1.527 1.421 Compound K 0.004 0.103 0.187 Rh2 0.099 2.087 2.757 Rh3 0.000 0.526 0.964 Rk2 0.000 0.656 1.062

Example 4: Analyze the content of 1-glycoside ginsenoside in red ginseng concentrate and steamed ginseng leaves

In order to compare the 1-glycoside ginsenoside content of the commercially available red ginseng concentrate and the steamed ginseng leaves of the present invention, the aim was to determine the commercially available Zhengguanzhuang red ginseng concentrate 1, the commercially available Fengji ginseng red ginseng concentrate Liquid 2, the commercially available Datong Korean ginseng red ginseng concentrate 3 and the 1-glycoside ginsenoside content of the ginseng leaves after the steaming treatment. The specific experimental method is as follows.

As shown in Table 5 below, the contents of 1-glycoside ginsenoside F1, compound K, Rh2, Rh3, or Rk2 of red ginseng concentrates 1 to 3 and steamed processed ginseng leaves were measured.

[table 5] (mg/g) Red ginseng concentrate 1 Red Ginseng Concentrate 2 Red Ginseng Concentrate 3 Steamed processed ginseng leaves F1 0.005 0.017 0.034 1.421 Compound K 0.002 0.004 0.009 0.187 Rh2 0.006 0.030 0.025 2.757 Rh3 0.002 0.006 0.002 0.964 Rk2 0.002 0.006 0.007 1.062

As shown in Table 4 to Table 5 above, ginsenosides contained in large amounts in all ginsenosides found in the ginseng leaf steamed composition and five existing 1-glycoside ginsenosides (1-glycoside ginsenoside) (F1, compound K, Rh2) , Rh3 or Rk2) are shown in Table 4, and Table 5 is added to compare five 1-glycoside ginsenosides (F1, compound K, Rh2, Rh3 or Rk2), and it can be confirmed that it is concentrated with dried ginseng leaves and commercially available red ginseng Compared with liquid 1 to 3, the content of five 1-glycosides ginsenosides in steamed ginseng leaves was higher. Therefore, it can be confirmed that the 1-glycoside ginsenoside can be selectively produced in large quantities by the present invention.

Example 5: Analysis of ginseng leaf 1-glycoside ginsenoside content according to steaming treatment time

The aim was to determine the change in the content of 1-glycoside ginsenoside according to the steaming treatment time. Specifically, the steaming time of ginseng leaves is processed for 1 hour to 7 hours, thereby measuring the content of 1-glycoside ginsenoside F1, compound K, Rh2, Rh3, or Rk2.

[Table 6] (mg/g) 1 hour 3 hours 5 hours 7 hours F1 1.073 1.237 1.527 1.472 Compound K 0.011 0.073 0.103 0.121 Rh2 0.315 1.258 2.087 2.125 Rh3 0.073 0.261 0.526 0.571 Rk2 0.095 0.451 0.656 0.538

As shown in Table 6 above, it can be confirmed that the content of 1-glycoside ginsenoside F1, compound K, Rh2, Rh3, or Rk2 increases with the steaming treatment time. However, when the steaming treatment time is more than 5 hours, the content does not change much. Therefore, considering the overall efficiency, it can be confirmed that the optimal steaming treatment time is 5 hours.

Experimental example: the activity of inhibiting skin darkening of ginseng leaf concentrate

In order to confirm the effect of the ginseng leaf concentrate of the present invention in inhibiting skin darkening, the melanin value was confirmed after ingesting the ginseng leaf concentrate. The specific experimental method is as follows.

Twenty women in their 30s to 50s suffering from dark spots took 3 g of the ginseng leaf concentrate prepared by the above preparation example every day, and after 8 weeks, the test was performed using a melanin skin detection device (Mexmeter). Questionnaire about deliberately reducing melanin value and improving dark spots.

According to the numerical value and questionnaire survey results, the melanin index of 20 subjects decreased after 8 weeks, and finally after 8 weeks, for the questionnaires to evaluate the efficacy and favorability of the product, all users were The degree of spot improvement and overall satisfaction with the product were all answered as good and very good, thus confirming the effect on all subjects. Therefore, the hydroponic ginseng leaf concentrate according to the present invention suppresses skin darkening, and can expect skin whitening improvement effects.

In summary, the hydroponic cultivated ginseng leaf concentrate of the present invention can provide a ginseng leaf concentrate with increased content of low-glycoside ginsenoside (1-glycoside ginsenoside) with high absorption rate in the body. In addition, the ginseng leaf concentrate of the present invention, which uses hydroponic ginseng leaves for a steaming process, can be provided as a food composition and a food composition for skin whitening and dark spot improvement.

Based on the above description, those skilled in the art to which the present invention belongs can understand that the present invention can also be implemented in other specific ways without changing the technical idea or necessary features of the present invention. In this regard, it should be understood that the embodiments described above are illustrative and not restrictive in all aspects. The scope of the present invention should be construed as including the meaning and scope of the scope of patent application described later, and all changes or modifications derived from the equivalent concept thereof, and not only the above detailed description.

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Claims (10)

A preparation method of ginseng leaf concentrate with increased content of 1-glycoside ginsenoside, comprising: The step of steaming the water-removed hydroponic ginseng leaves; The step of drying and crushing the steamed ginseng leaves; and The step of extracting and concentrating the dried and crushed ginseng leaves. The method for preparing a ginseng leaf concentrate according to claim 1, wherein the 1-glycoside ginsenoside is one or more selected from the group consisting of ginsenoside F1, compound K, Rh2, Rh3, and Rk2. The method for preparing a ginseng leaf concentrate according to claim 1, wherein the steaming treatment is performed for 4 hours to 6 hours. The method for preparing a ginseng leaf concentrate according to claim 1, wherein the above-mentioned steaming treatment is carried out at 90°C to 110°C. The method for preparing a ginseng leaf concentrate according to claim 1, wherein the hydroponic cultivated ginseng leaves are 1 to 3 years old. The method for preparing a ginseng leaf concentrate according to claim 1, wherein the drying is performed so that the moisture of the ginseng leaf is between 1% and 15%. The method for preparing a ginseng leaf concentrate according to claim 1, wherein the extraction step is performed by adding alcohol. A method for increasing the content of 1-glycoside ginsenoside when preparing ginseng leaf concentrate, comprising: The step of steaming the water-removed hydroponic ginseng leaves; The step of drying and crushing the steamed ginseng leaves; and The step of extracting and concentrating the dried and crushed ginseng leaves. The method according to claim 1, wherein the 1-glycoside ginsenoside is one or more selected from the group consisting of ginsenoside F1, compound K, Rh2, Rh3, and Rk2. A ginseng leaf concentrate prepared by the method described in any one of claims 1 to 7.