TW202122799A - 分離循環核小體之方法 - Google Patents

分離循環核小體之方法 Download PDF

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TW202122799A
TW202122799A TW109129345A TW109129345A TW202122799A TW 202122799 A TW202122799 A TW 202122799A TW 109129345 A TW109129345 A TW 109129345A TW 109129345 A TW109129345 A TW 109129345A TW 202122799 A TW202122799 A TW 202122799A
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Taiwan
Prior art keywords
dna
nucleosomes
protein
nucleosome
binding
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TW109129345A
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English (en)
Chinese (zh)
Inventor
馬克 愛德華 愛寇斯頓
雅各 文森 米考夫
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比利時商比利時意志有限公司
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Priority claimed from GBGB1912251.4A external-priority patent/GB201912251D0/en
Priority claimed from GBGB1916735.2A external-priority patent/GB201916735D0/en
Priority claimed from GBGB2006547.0A external-priority patent/GB202006547D0/en
Application filed by 比利時商比利時意志有限公司 filed Critical 比利時商比利時意志有限公司
Publication of TW202122799A publication Critical patent/TW202122799A/zh

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    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
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    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/575—Immunoassay; Biospecific binding assay; Materials therefor for cancer
    • G01N33/5758—Immunoassay; Biospecific binding assay; Materials therefor for cancer involving compounds serving as markers for tumours, cancers or neoplasias, e.g. cellular determinants, receptors, heat shock/stress proteins, A-protein, oligosaccharides or metabolites
    • G01N33/57585—Immunoassay; Biospecific binding assay; Materials therefor for cancer involving compounds serving as markers for tumours, cancers or neoplasias, e.g. cellular determinants, receptors, heat shock/stress proteins, A-protein, oligosaccharides or metabolites involving compounds identifiable in body fluids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6875—Nucleoproteins
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/131—Nucleic acid detection characterized by the use of physical, structural and functional properties the label being a member of a cognate binding pair, i.e. extends to antibodies, haptens, avidin
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/50—Detection characterised by immobilisation to a surface
    • C12Q2565/531—Detection characterised by immobilisation to a surface characterised by the capture moiety being a protein for target oligonucleotides
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    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/154—Methylation markers
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    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers

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  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
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  • Wood Science & Technology (AREA)
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  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Pathology (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Oncology (AREA)
  • Hospice & Palliative Care (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
TW109129345A 2019-08-27 2020-08-27 分離循環核小體之方法 TW202122799A (zh)

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
GB1912251.4 2019-08-27
GBGB1912251.4A GB201912251D0 (en) 2019-08-27 2019-08-27 Method of isolating circulating nucleosomes
GBGB1916735.2A GB201916735D0 (en) 2019-11-18 2019-11-18 Method of isolating circulating nucleosomes
GB1916735.2 2019-11-18
GB2006547.0 2020-05-04
GBGB2006547.0A GB202006547D0 (en) 2020-05-04 2020-05-04 Method of isolating circulating nuccleosomes

Publications (1)

Publication Number Publication Date
TW202122799A true TW202122799A (zh) 2021-06-16

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Family Applications (1)

Application Number Title Priority Date Filing Date
TW109129345A TW202122799A (zh) 2019-08-27 2020-08-27 分離循環核小體之方法

Country Status (12)

Country Link
US (1) US12571051B2 (https=)
EP (1) EP4022312A1 (https=)
JP (1) JP2022545821A (https=)
KR (1) KR20220111246A (https=)
CN (1) CN114901832A (https=)
AU (1) AU2020336928A1 (https=)
BR (1) BR112022003546A2 (https=)
CA (1) CA3152642A1 (https=)
IL (1) IL290879A (https=)
MX (1) MX2022002458A (https=)
TW (1) TW202122799A (https=)
WO (1) WO2021038010A1 (https=)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2913665T3 (es) 2017-09-18 2022-06-03 Santersus Ag Método y dispositivo para la purificación de sangre a partir de ADN celular libre en circulación
EP4041914A1 (en) * 2019-10-04 2022-08-17 Santersus AG Method for isolating and analyzing cell free dna
TW202238131A (zh) 2020-11-25 2022-10-01 比利時商比利時意志有限公司 測量無細胞核蛋白染色質片段之方法
TW202242145A (zh) 2020-12-29 2022-11-01 比利時商比利時意志有限公司 核小體耗盡循環無細胞染色質片段之轉錄因子結合位點分析
GB202108185D0 (en) * 2021-06-08 2021-07-21 Belgian Volition Sprl Standardisation of nucleosome assays using biologically derived calibrants
WO2023126672A1 (en) 2021-12-27 2023-07-06 Santersus Ag Method and device for removal of circulating cell free dna
WO2023170298A1 (en) * 2022-03-11 2023-09-14 Belgian Volition Srl Differential diagnosis method
WO2024049928A1 (en) * 2022-08-30 2024-03-07 Northeastern University Epigenetic analysis after single cell interactions using microfluidics
EP4639173A1 (en) * 2022-12-19 2025-10-29 Belgian Volition SRL Calibration
GB202300607D0 (en) 2023-01-16 2023-03-01 Belgian Volition Srl Proteins and methods for isolating circulating nucleosomes
EP4689160A1 (en) * 2023-04-03 2026-02-11 Belgian Volition SRL Recombinant nucleosome materials
WO2025007034A1 (en) * 2023-06-29 2025-01-02 Guardant Health, Inc. Methods for determining surveillance and therapy for diseases
WO2025061853A1 (en) 2023-09-19 2025-03-27 Belgian Volition Srl Lateral flow test device
WO2025073909A1 (en) 2023-10-04 2025-04-10 Belgian Volition Srl Pcr method for detecting ctcf occupied cfdna fragments

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2446292A1 (en) * 2001-05-04 2003-06-26 Incyte Genomics, Inc. Nucleic acid-associated proteins
GB0319376D0 (en) 2003-08-18 2003-09-17 Chroma Therapeutics Ltd Histone modification detection
GB201115099D0 (en) * 2011-09-01 2011-10-19 Belgian Volition Sa Method for detecting nucleosomes
GB201115095D0 (en) 2011-09-01 2011-10-19 Singapore Volition Pte Ltd Method for detecting nucleosomes containing nucleotides
GB201115098D0 (en) 2011-09-01 2011-10-19 Belgian Volition Sa Method for detecting nucleosomes containing histone variants
CN104067125B (zh) 2011-12-07 2017-12-26 比利时意志有限责任公司 用于检测核小体加合物的方法
HK1206055A1 (en) 2012-03-02 2015-12-31 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
AU2015340378B2 (en) * 2014-10-29 2021-08-12 Belgian Volition Sprl Method for the enrichment of circulating tumor DNA
LT3334841T (lt) * 2015-08-12 2020-02-10 Cemm - Forschungszentrum Für Molekulare Medizin Gmbh Nukleorūgščių tyrimo būdai
GB201518665D0 (en) * 2015-10-21 2015-12-02 Singapore Volition Pte Ltd Method for enrichment of cell free nucleosomes
EP4339288A3 (en) * 2016-03-18 2024-06-05 Caris Science, Inc. Oligonucleotide probes and uses thereof
CA3046007A1 (en) 2016-12-22 2018-06-28 Guardant Health, Inc. Methods and systems for analyzing nucleic acid molecules
ES2913665T3 (es) * 2017-09-18 2022-06-03 Santersus Ag Método y dispositivo para la purificación de sangre a partir de ADN celular libre en circulación

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Publication number Publication date
US12571051B2 (en) 2026-03-10
JP2022545821A (ja) 2022-10-31
CN114901832A (zh) 2022-08-12
AU2020336928A1 (en) 2022-03-24
KR20220111246A (ko) 2022-08-09
MX2022002458A (es) 2022-05-19
US20220290252A1 (en) 2022-09-15
IL290879A (en) 2022-04-01
CA3152642A1 (en) 2021-03-04
EP4022312A1 (en) 2022-07-06
BR112022003546A2 (pt) 2022-05-17
WO2021038010A1 (en) 2021-03-04

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