TW202023370A - The method for large scale culturing ofnoctilucascintillans - Google Patents

Abstract

The present invention provides a method for the large-scale culture of Noctiluca scintillans, which uses a monitoring system of Noctiluca scintillans culture. The system includes a monitoring unit and a culturing unit. The culturing unit sequentially includes an automatic culture medium supply device, a bait incubator, a Noctiluca scintillans incubator and a Noctiluca scintillans storage tank, and is also provided with a pipeline used for connection. The monitoring unit includes: a temperature controller, a salinity controller, a luminosity controller and a bait concentration sensor. The bait concentration sensor is arranged in the bait incubator and the Noctiluca scintillans incubator. The method includes the step of using a fluorescence probe to measure chlorophyll fluorescence as an index to be converted into a bait concentration value, thereby adjusting the flow rates of the culture medium and the bait.

Description

Mass cultivation method of noctiluca

The present invention relates to a large-scale cultivation method of noctiluca.

Since noctiluca has no edible and commercial value, the cultivation of noctiluca so far has been limited to small-scale laboratory cultivation and breeding. The current laboratory practice is to change the medium regularly, transfer part of the noctiluca in the culture flask directly to the new medium, and discard the old medium and noctiluca. This kind of cultivation method focuses on short-term experiments or long-term preservation of seeds, so the water quality control and automatic supply of bait are completely ignored. This kind of cultivation method is only suitable for small-scale cultivation and cannot meet the large-scale demand for commercial display.

In view of the above problems, the inventor of this case proposes a method for mass cultivation of noctilucas, using a noctilucas cultivation monitoring system (Figure 1), which uses temperature, salinity, luminosity and bait concentration sensors to control the growth of noctilucas and the feeding environment Condition, can automatically cultivate bait and monitor and maintain the culture environment to cultivate noctiluca in a large amount.

The content of the present invention is as follows: [1] A method for mass cultivation of noctilucas, using a noctilucas cultivation monitoring system, the system includes a monitoring unit and a cultivation unit, the monitoring unit includes a temperature controller, a salinity controller, and luminosity A controller and a bait concentration sensor; the culture unit includes an automatic medium supply device, a bait incubator, a noctiluca incubator, and a noctilucas storage box in sequence, and has a pipeline for communication; the bait incubator and the noctilucas The incubator is equipped with the bait concentration sensor, and the method includes the following steps: the bait concentration sensor uses a fluorescent probe to measure the bait concentration value, and the bait concentration sensor measures the bait concentration value in the bait incubator, by This adjusts the flow rate of the medium flowing out of the automatic medium supply device; and measures the concentration of the bait in the noctiluca incubator with the bait concentration sensor, thereby adjusting the flow rate of the bait out of the bait incubator.

[2] The method for mass cultivation of Noctiluca in [1], wherein the culture unit further includes a culture medium control valve, when the bait concentration sensor measures the bait concentration in the bait incubator and judges that the bait concentration fluctuates, Start the medium control valve, and control the growth rate of the bait organisms by adjusting the flow of the medium.

[3] As in [1], the method for mass cultivation of noctiluca, wherein the monitoring unit further includes a thermostat, a salt and fresh water control valve, the temperature controller is used to measure the temperature in the bait incubator and the noctiluca incubator, and borrow The temperature is adjusted by the thermostat to maintain the temperature within a predetermined range, the salinity controller is used to measure the salinity in the bait incubator and the noctilucent incubator, and the brackish water control valve is used to adjust the inflow of the brackish water to make The salinity is maintained within a predetermined range.

Other implementation modes and advantages of the present invention will be more apparent from the following detailed description combined with the accompanying drawings used to illustrate examples of the principles of the present invention. In addition, in order not to cause unnecessary confusion to the present invention, the well-known principles will not be repeated in this specification.

The structure of the noctiluca cultivation monitoring system 1 is shown in FIG. 1, the noctiluca cultivation monitoring system 1 may include a monitoring unit and a cultivation unit, which may be set on a general desk or a cultivation rack. The cultivation unit includes an automatic medium supply 11, a bait incubator 12, a noctiluca incubator 7, and a noctiluca storage box 8, in order, and has a pipeline for communication. The monitoring unit includes: a temperature controller 2 for measuring and controlling the temperature of the water body by a thermostat; a salinity controller 3 for measuring the salinity of the water body and adjusting the inflow of salt and fresh water by a salt water control valve 9 ; To control the salinity in the Noctiluca incubator 7 and the bait incubator 12 (Figure 2); the luminosity controller 4 is used to measure the water and control the Noctiluca incubator 7 and the bait incubator 12 (Figure 2) The luminosity; bait concentration sensor 5, monitor the concentration of bait. Noctilucent incubator 7 is connected with temperature controller 2, salinity controller 3, luminosity controller 4, and bait concentration sensor 5. At the same time, it uses bait concentration sensor 5 and uses fluorescent probe to measure chlorophyll fluorescence as indicator monitoring Feed concentration; measure the concentration of the bait in the noctiluca incubator 7, adjust the bait regulating valve 10 accordingly to control the flow of bait out of the incubator 12; measure the concentration of the bait in the bait incubator 12, and control the medium control The valve 13 adjusts the flow rate of the medium flowing out of the automatic medium supply 11. The noctiluca storage box 8 and the noctiluca incubator 7 are directly connected via a pipeline, and the liquid containing noctilucas cultivated by the noctilucas incubator 7 slowly drips into the noctiluca storage box 8, which can be taken out from the noctiluca storage box 8 to provide display use. The growth rate of noctiluca is controlled by the temperature controller 2, the salinity controller 3, the luminosity controller 4, and the bait regulating valve 10 of the noctiluca incubator 7. In the embodiment of the present invention, the bait automatic culture supply part 6 mainly composed of the bait incubator 12 and the bait concentration sensor 5 can provide various bait organisms such as green algae (Ply) or diatoms for feeding by noctiluca.

<Bait> In the embodiment of the present invention, the bait organisms for nocturnal insects are green algae (Ply) and diatoms. As shown in Fig. 2, the overall arrangement diagram of the actual device of the bait automatic culture supply part 6 of the noctiluca culture monitoring system 1 of the present invention is presented to illustrate the culture medium control valve 13 and the bait culture automatic supplier 11 when the bait is cultivated. , And the relative configuration relationship between the position of the bait incubator 12 and its controller. The bait automatic cultivation supply part 6 can automatically cultivate bait and supply the bait demand in the noctiluca incubator 7. The bait automatic cultivation supply unit 6 is mainly composed of two parts, one is the bait incubator 12, which is automatically controlled by the temperature controller 2, the salinity controller 3 and the luminosity controller 4 to automatically control the growth temperature, salinity and luminosity; the other is the bait concentration The sensor 5 uses a fluorescent probe to measure the chlorophyll fluorescence as an indicator to monitor the concentration of the bait, and accordingly controls the medium control valve 13 to regulate the flow of the medium, thereby controlling the growth rate of the bait. In this embodiment, the culture conditions of the bait organisms are controlled at a temperature of 23° C., a salinity of 33-35, an illumination of 2000-3000 Lux, and an illumination interval of 12 hours, black and 12 hours bright. Fluorescent probes will vary with the brand and use time value, so basically when you get the probe, you must first calibrate it to understand the relationship between the chlorophyll concentration value measured by the probe and the actual chlorophyll concentration value, and then it needs to be weekly Perform the same calibration steps to ensure the reliability of the probe. Basically, a monitoring value can be automatically provided every ten minutes. When the difference between the value and the average chlorophyll concentration in the bait incubator 12 within a week exceeds 50%, the culture medium control valve 13 is opened to replenish the culture medium to the bait incubator 12.

<Noctilucent worm> The operating method of the noctiluca cultivation monitoring system 1 may include the following steps: use the temperature controller 2 to measure and control the temperature of the water body; use the salinity controller 3 to measure and control the salinity of the water body; use the luminosity controller 4 to provide The light source allows the bait to undergo photosynthesis; the bait concentration sensor 5 is used to measure the concentration of the bait in the water body and continues to provide the water body bait; the noctiluca incubator 7 receives the biological (existing) value measured by the noctiluca storage box 8 and judges Whether the number of luminous insects has increased.

5A and 5B are schematic diagrams illustrating the number and time changes of Noctiluca spp. in the Noctiluca spp. cultivation monitoring system 1 shown in continuous culture (Fig. 3) and semi-continuous culture (Fig. 4). As shown in Figure 5A, the number of noctilucas in the noctiluca storage box 8 is counted by a microscope, and the measured number of noctilucas has a smooth curve with time and no fluctuations. This is because the bait is adjusted The valve 10 is adjusted to continuous cultivation, that is, to control the continuous and stable supply of bait, so the concentration of noctiluca in the noctiluca storage box 8 will not change, and the number of noctilucas steadily increases. On the other hand, as shown in Figure 5B, the measured number of noctilucas fluctuates. This is because the bait regulating valve 10 is adjusted to semi-continuous culture, that is, a fixed amount of bait is given for a fixed time, so every time When supplying bait, the concentration of bait in the water is high, and the growth rate of noctiluca is increased, but with time the concentration of the bait will decrease rapidly as the noctilucas eats, and the growth rate of noctilucas will also decrease with the concentration of the bait, so noctilucas storage The concentration of Noctiluca in Box 8 will also change. Therefore, it can be judged whether the measured number of noctilucas fluctuates or whether the measured number of noctilucas reaches a predetermined threshold, and the temperature controller 2 and the salinity controller 3 can be adjusted according to the determined result. , Luminosity controller 4, bait regulating valve 10 to adjust the noctiluca incubator culture conditions in the noctiluca incubator 7.

Due to the material of the incubator, the size of the culture water, and the type of bait (such as diatoms, green algae, etc.) may cause biological carrying capacity for the cultivation of noctilucas, so noctilucas cultivation monitoring system may occur 1 The growth efficiency of the organism is inconsistent in each culture environment. In addition, as described above, when the cultivation progresses, the noctiluca biomass produced by the noctiluca cultivation monitoring system 1 fluctuates with the cultivation time. Therefore, the noctiluca biomass value measured by the microscope can be measured over time. The change in the growth curve is used to determine the best conditions for the noctiluca cultivation monitoring system 1. If the noctiluca cultivation monitoring system 1 reaches the conditions for the best production efficiency of noctiluca biomass with the cultivation time, the bait regulating valve 10 at the end of the bait automatic cultivation part 6 will have a fixed opening frequency or flow rate, so that continuous observation and adjustment are not required The amount of bait supplement only needs to be monitored once a day for the growth of noctiluca, which can greatly reduce the consumption of personnel. In this embodiment, the culture conditions of Noctilaria are temperature 23-25°C and salinity 33-35.

Although the present invention has been described in detail with reference to the preferred embodiments and drawings, those skilled in the art can understand that various modifications, changes and equivalent substitutions can be made without departing from the spirit and scope of the present invention. However, these Modifications, changes and equivalent substitutions still fall within the scope of the patent application of the present invention.

[Industrial Applicability] With the present invention, noctiluca can be cultivated in large quantities, and large-scale demand for commercial display can be provided, and it has industrial applicability.

1: Noctiluca cultivation monitoring system 2: Temperature controller 3: Salinity controller 4: Luminosity controller 5: Bait concentration sensor 6: Bait automatic cultivation supply department 7: Noctiluca incubator 8: Noctiluca storage box 9: Salt and fresh water control valve 10: bait regulating valve 11: medium automatic supply 12: bait incubator 13: medium control valve

Figure 1 is a schematic diagram showing the structure of the Noctiluca spp. culture monitoring system.

Figure 2 shows the overall configuration diagram of the noctiluca culture monitoring system.

Fig. 3 is a schematic diagram of the relationship between the growth rate of noctilucas and time when the bait regulating valve is adjusted to continuous cultivation, that is, the continuous and stable supply of bait is controlled using the noctilucas culture monitoring system of the present invention.

Figure 4 is a schematic diagram of the relationship between the growth rate of noctilucas and the time when the bait regulating valve is adjusted to semi-continuous cultivation, that is, when a fixed amount of bait is given for a fixed time using the noctilucas culture monitoring system of the present invention. Figures 5A and 5B are graphs showing the changes in the number of noctilucas over time in the continuous culture and semi-continuous culture, respectively.

1: Noctiluca culture monitoring system

2: Temperature controller

3: Salinity controller

4: Luminosity Controller

5: Bait concentration sensor

6: Bait automatic cultivation and supply department

7: Noctilucent incubator

8: Noctilucent worm storage box

9: Salt and fresh water control valve

10: Bait control valve

11: Automatic medium supply device

12: Bait incubator

13: Medium control valve

Claims (3)

A method for mass cultivation of noctilucas uses the following system, the system includes a monitoring unit and a cultivation unit, the monitoring unit includes a temperature controller, a salinity controller, a luminosity controller, and a bait concentration sensor; the cultivation unit In order, it includes an automatic medium supply device, a bait incubator, a noctiluca incubator, and a noctiluca storage box, and has a pipeline for communication; the bait incubator and the noctiluca incubator are equipped with the bait concentration sensor, 　　The method includes the following steps: The bait concentration sensor uses a fluorescent probe to measure the bait concentration value, and the bait concentration sensor measures the bait concentration value in the bait incubator, thereby adjusting the flow rate of the medium from the automatic medium supply Using the bait concentration sensor to measure the bait concentration value in the noctiluca incubator, thereby adjusting the flow of bait out of the bait incubator. For example, the method for mass cultivation of noctiluca in the scope of the patent application, wherein the cultivation unit further includes a medium control valve, when the bait concentration sensor measures the bait concentration in the bait incubator and judges that the bait concentration fluctuates, Start the medium control valve, and control the growth rate of the bait organisms by adjusting the flow of the medium. For example, the method for mass cultivation of noctiluca in the scope of the patent application, wherein the monitoring unit further includes a thermostat, a salt and fresh water control valve, 　　 use the temperature controller to measure the temperature in the bait incubator and noctiluca incubator, and borrow The temperature is adjusted by the thermostat to maintain the temperature in a predetermined range, and the salinity controller is used to measure the salinity in the bait incubator and the noctilucent incubator, and the brackish water control valve is used to adjust the inflow of the brackish water to make The salinity is maintained within a predetermined range.

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