TW201909891A - Amino acid composition for promoting noradrenaline secretion - Google Patents

Abstract

The subject of the present invention is to provide a novel amino acid composition capable of promoting secretion of adrenaline and/or noradrenaline. The present invention relates to an amino acid composition for promotion of noradrenaline secretion having arginine, valine and serine. Particularly, the present invention relates to an amino acid composition for the promotion of noradrenaline secretion having a weight ratio of arginine, valine and serine of 50~70: 25~50: 0.1~15, and a food, a beverage or a medicine for promoting the secretion of noradrenaline comprising an effective amount of the amino acid composition.

Description

Amino acid composition for promoting the secretion of norepinephrine

The present invention relates to an amino acid composition for promoting the secretion of norepinephrine and a food, a drink and a pharmaceutical containing the same.

A lot of research has been conducted on the relationship between the amino acid composition and the fatigue feeling accompanying exercise function or exercise. For example, an amino acid composition comprising 18 amino acids similar to the composition of the amino acid unit constituting casein has the function of promoting burning fat, improving endurance, preventing, reducing or restoring fatigue caused by exercise, and anti-obesity. Anti-stress and other effects (Patent Document 1), a composition composed of 17 kinds of amino acids contained in saliva secreted by hornet larvae (VAAM) is known to have sustainable muscle strength, nourish and strengthen, and is used for blood transfusion and supplement nutrition. Recovering fatigue and regulating the production of lactic acid (Patent Document 2). Further, it is known that the addition of branic acid to VAAM enhances the oxygen-free exercise accompanying a strong load (Patent Document 3).

It is known that VAAM promotes the secretion of adrenaline and/or norepinephrine, thereby acting as a blood pressure, maintaining blood sugar, improving motor function, maintaining tension, promoting and preventing fat decomposition and hyperlipidemia in obese people. The therapeutic drug for the disease is useful (Patent Document 4). Although the mechanism of secretion of epinephrine and norepinephrine from VAAM is not fully understood, tyrosine or phenylalanine in VAAM synthesizes adrenaline and norepinephrine in the adrenal gland, assuming secretion in the blood. To date, the use of amino acid compositions has not been fully investigated to promote the secretion of adrenaline and/or norepinephrine. [Prior Technical Literature] [Patent Literature]

[Patent Document 1] International Publication No. 2008-105368 [Patent Document 2] [Patent Document No. 2, 528, 432, [Patent Document 3]

[Problems to be Solved by the Invention] Therefore, the problem of the present invention is to provide a novel amino acid composition which can promote the secretion of adrenaline and/or norepinephrine. [Means for solving problems]

In order to solve the aforementioned problems, the inventors of the present invention focused on arginine, valine and serine in an effort to find that it promotes the secretion of norepinephrine in an amino acid composition containing only these three amino acids. Further, the present invention has been completed as a result of further research on promoting adrenaline secretion.

That is, the present invention relates to the following invention. [1] An amino acid composition for promoting secretion of norepinephrine, which is composed of arginine, valine and serine. [2] The amino acid composition according to the above [1], wherein the weight ratio of arginine, valine and serine is from 50 to 70:25 to 50:0.1 to 15. [3] The amino acid composition according to the above [1], wherein the weight ratio of arginine, valine and serine is from 10 to 28:7 to 22:1. [4] The amino acid composition according to any one of [1] to [3] above which is contained in an edible container. [5] A food, a drink or a pharmaceutical product for promoting the secretion of norepinephrine, comprising an effective amount of the amino acid composition according to any one of the above [1] to [4]. [6] The food, drink or pharmaceutical according to the above [5], which comprises a non-effective amount of an amino acid other than arginine, proline or serine. [7] The food, drink or pharmaceutical according to the above [5] or [6], which further comprises an active ingredient for promoting adrenaline and/or norepinephrine secretion by a non-amino acid. [8] The food, drink or pharmaceutical according to any one of the above [5] to [7], which is contained in a container. The method for producing a food, beverage, or pharmaceutical according to any one of the above [1] to [4], wherein the food, beverage, or pharmaceutical product according to any one of the above [5] to [8] Contains: mixed arginine, valine and serine. [Effect of the invention]

According to the present invention, it is possible to provide an amino acid composition, a food, a drink, and a pharmaceutical containing the amino acid composition, and to have an effect of a concentration of norepinephrine in the ascending blood during exercise. Further, the amino acid composition of the present invention tends to increase adrenaline in the blood. In particular, adrenaline and norepinephrine are all adrenal medullas and activate sympathetic nerves. Therefore, the present invention can be expected to promote catabolism, blood pressure rise, and brain arousal. Further, by ingesting the amino acid composition of the present invention or the like before exercise, the concentration of norepinephrine in the blood and/or blood in the exercise increases, and the exercise performance is improved.

The amino acid composition for promoting the secretion of norepinephrine of the present invention is composed of three types of arginine, lysine and serine. The weight ratio of arginine, valine and serine is 50 to 70:25 to 50:0.1 to 15 in one aspect, and 10 to 28:7 to 22:1 in other aspects. It is preferably 15 to 18:11 to 14:1. By doing this weight ratio, the secretion promoting effect is further achieved.

The amino acid composition of the present invention, in one aspect, is contained in a container, preferably in an edible container. The edible container refers to a capsule for formulation and the like. The amino acid composition contained in the container can be directly added to foods, beverages, and pharmaceuticals by using an edible container.

The amino acid composition of the present invention has an effect of promoting secretion, and can be used in an effective amount in a food, a drink or a pharmaceutical. A food or beverage to which an effective amount of an amino acid composition is added becomes a composition different from a food or beverage directly existing in nature. The effective amount as such may be appropriately determined by those skilled in the art, for example, the total weight of arginine, valine and serine is from 500 mg to 10,000 mg, preferably from 1500 mg to 5000 mg, particularly preferably from 2500 mg to 3500 mg.

The food, drink or pharmaceutical of the present invention may also contain an amino acid other than arginine, lysine or serine. In the present invention, an amino acid other than arginine, valine and serine may be used to promote secretion, and may also contain, for example, an amount (non-effective amount) which is not effective for achieving a secretion-promoting effect. An ineffective amount like this can be appropriately determined by those skilled in the art.

The amino acid used in the present invention may also be any one of an L-form, a D-form, and a DL-form, and is preferably an L-form, a DL-form, or more preferably an L-form. Further, the amino acids used in the present invention may each be not only a free form but also an amino acid salt. The amino acid salt is not particularly limited as long as it is an amino acid salt which can be tolerated as a food, a drink or a pharmaceutical, and examples thereof include an acid addition salt of an amino acid or a salt with a base.

As the salt of the food which can be tolerated as the amino acid used in the present invention, as the acid forming the salt, for example, inorganic acids such as hydrochloric acid, hydrogen bromide, sulfuric acid, phosphoric acid, etc.; acetic acid, lactic acid, citric acid, tartaric acid , organic acids such as maleic acid, fumaric acid, and monomethyl sulfuric acid. Further, examples of the base which can be used as a salt of a food include an inorganic base such as sodium, potassium, calcium or ammonia, or ethylenediamine, propylenediamine, ethanolamine, monoalkylethanolamine, dialkylethanolamine or diethanolamine. Organic bases such as triethanolamine. The amino acid salt used in the present invention may also be a hydrate (hydrated salt), and as such a hydrate, for example, a hydrate of 1 to 6 is exemplified.

The amino acid used in the present invention may be any one obtained by extracting and purifying animals, plants, microorganisms containing these, or by chemical synthesis, fermentation, or genetic recombination. Also, it is also commercially available. The amino acid used in the present invention is preferably one which is used alone in high purity. For example, an amino acid having a purity equal to or higher than that specified in the "Japanese Food Additives" is used. Further, those having a physiologically acceptable salt can also be used as these amino acids.

In the production of the amino acid composition of the present invention, the above amino acid may also be mixed. Further, when the solution is produced, it may be mixed and dissolved with distilled water, ethanol or the like. The food, drink or pharmaceutical of the present invention may further comprise an active ingredient for muscle strength enhancement of a non-amino acid. As such an active ingredient, for example, protein or vitamin D is mentioned.

The ingredient which can be contained in the food, drink or pharmaceutical of the present invention is not particularly limited as long as it can be ingested (administered) into the body, but is usually a component mixed with a pharmaceutical product and/or a food or drink, and can be blended, for example. Vitamin B1, vitamin B2, vitamin B6, pantothenic acid and other vitamins, coenzyme Q10, carnitine, alpha-lipoic acid and other vitamins, iron, zinc and other minerals, glucose, fructose, galactose, mannose, sucrose, maltose , sugars such as lactose, organic acids such as citric acid, tartaric acid, ascorbic acid, malic acid, acetic acid, oxalic acid, phosphoric acid, lactic acid, butyric acid, succinic acid, fumaric acid and gluconic acid, caffeine, theobromine, theanine, Polyphenols such as catechins.

Further, an amino acid component such as sodium glutamate or taurine, sucralose, neotame, ethyl sulfamate, thaumatin, stevia extract, licorice extract, mangosteen extract, saccharin, sodium saccharin, and the like may be mixed. Sweeteners such as glycyrrhizic acid, disodium glycyrrhizinate, trisodium glycyrrhizinate, dihydrochalcone, stevioside, stevioside A, minerals such as zinc, selenium, iron, copper, trehalose, oligosaccharides, rare Sugars such as sugar, lactic acid bacteria, Lactobacillus brevis, Propionibacterium, and other useful microorganisms or cultures thereof, and existing functional materials such as royal jelly, collagen, ceramide, glucosamine, reduced astaxanthin, polyphenols, and the like. , pH adjusters, excipients, acidifiers such as citric acid, colorants, emulsifiers, preservatives, and the like.

The method for ingesting (administering) the amino acid composition of the present invention is not limited as long as it can be ingested (administered) into the body, but is preferably a tablet, a coated tablet, a capsule, or a granule. , powder, solution, syrup, emulsion, etc. are administered orally. These various preparations can be used in the field of pharmaceutical preparations such as excipients, binders, disintegrants, lubricants, coloring agents, flavoring agents, solubilizing agents, suspending agents, coating agents, etc. according to a conventional method, and conventionally used auxiliary agents can be used. Formulated in the amino acid composition of the present invention.

In one aspect, the beverage food of the present invention may be used as a food and beverage (including a health food, a functional food, a nutritious food, and the like, and may be provided as a food and drink, etc.). Nutritional supplements, other health foods, supplements).

The beverage food of the present invention can be used as a dairy product such as a beverage or a yogurt drink, a jelly product, an ice cream, an ice product, a biscuit, a mouth gel product, a powder product, a granulated product, a sheet product, a capsule product. , solid products such as ingot products, candy, etc.

The food or drink of the present invention is not particularly limited as long as it is a solution, a suspension, an emulsion, a powder, a solid molded product, or the like, which can be orally ingested. Specifically, for example, dairy products such as milk beverages, yogurt drinks, lactic acid bacteria beverages, fermented milk, ice creams, creams, and cheeses; refreshing drinks, juice drinks, vegetable drinks, soy milk drinks, coffee drinks, and tea drinks are mentioned. , jelly drinks, cocoa, smoothies and other powder drinks or sports powder drinks, nutritionally enhanced powder drinks, powdered foods, powder soups, taro, concentrated drinks, alcoholic beverages, etc.; bread, pasta, noodles, cake powder, Wheat flour products such as fried flour and bread flour; desserts such as chocolate, chewing gum, candy, biscuits, soft candy, snacks, Japanese desserts, jellies, puddings, etc.; desserts such as processed seasonings, flavor seasonings, and comprehensive seasonings; Curry, curry, pasta sauce, fire pot, stew, Japanese food, sterilized bagged food; processed oils, butter, margarine, spreads, cannabis and other oils; freeze-dried foods and other fast-food foods; Agricultural canned food, jam, peel sauce, cooked beans, oysters, vegetable porridge and other agricultural products; aquatic products; processed products; Baked rice, frozen food macaroni gratin, home cooking, frying and the like; liquid diet, food thickening adjustment, as well as animal feed, troches and the like.

When the amino acid composition of the present invention is used in a food or drink, the other ingredients are not particularly limited, but the aforementioned foods and drinks may use water, protein, carbohydrates, lipids, vitamins, minerals, organic acids, and organic bases. Juices, flavorings, etc. are used as ingredients. The protein is exemplified by whole milk powder, skimmed milk powder, partially skimmed milk powder, casein, whey powder, whey protein, whey protein concentrate, whey protein isolate, α-casein, β-casein, kappa- Casein, β-lactoglobulin, α-lactalbumin, lactoferrin, soy protein, egg protein, meat protein, hydrolysate of these; butter, whey mineral, cream, whey, non-protein nitrogen, saliva Various milk-derived components such as acid, phospholipid, and lactose. Carbohydrates include sugars, processed starches (except dextrin, soluble starch, British starch, oxidized starch, starch esters, starch ethers, etc.), dietary fiber, and the like. As the lipid, for example, lard oil, fish oil, and the like, animal fat such as fractionated oil, hydrogenated oil, and transesterified oil; palm oil, safflower oil, corn oil, rapeseed oil, coconut oil, fractionated oil of these, hydrogenation Vegetable oils such as oil and transesterified oil. As vitamins, for example, vitamin A, carotene, vitamin B group, vitamin C, vitamin D group, vitamin E, vitamin K group, vitamin P, vitamin Q, nicotinic acid, pantothenic acid, biotin, inositol, Choline, folic acid, etc., as minerals, for example, calcium, potassium, magnesium, sodium, copper, iron, manganese, zinc, selenium, and the like. As the organic acid, for example, malic acid, citric acid, lactic acid, tartaric acid or the like is mentioned. These components may be used in combination of two or more kinds, and foods containing many synthetic products and/or these may also be used.

The food, the beverage, and the pharmaceutical product of the present invention may be contained in a container, and the container may be selected from the group consisting of plastic, paper, metal, ceramic, or composite materials thereof. It can be used as a loading container in these containers. That is, a food and beverage packaged in a container containing the amino acid composition of the present invention, and a pharmaceutical product filled in the container can be exemplified. Further, the container may be coated with a shrink film made of plastic, a light-shielding film (for example, a metal foil film, a black or dark ink coating film), or two or more types of the container or the film may be used in combination. From the viewpoint of deterioration of flavor and decomposition (reduction), a container or film having a light-shielding property is preferable.

The amino acid composition or the food or beverage of the present invention is composed of a unit package form per meal, and includes arginine, lysine, and serine in the unit package form. Here, the "unit packaging form per meal" refers to a form in which the amount of intake per meal is determined in advance, for example, as a foodstuff that can be orally ingested in a specific amount, not only a general food but also a beverage (such as a beverage). , health supplement foods, health functional foods, supplies, etc. In the case of "unit packaging form per meal", for example, in the case of a liquid beverage, a gel, a paste or a paste jelly, a powder, a granule, a capsule, or a solid food, Packaging containers such as metal cans, glass bottles (bottles, etc.), plastic containers (PET bottles, etc.), packaging, bags, film containers, cartons, etc., to specify a specific amount (amount), or by ingesting each meal The amount (usage, amount) indicates a form in which a specific amount is specified in a packaging container or a web page.

The daily intake of the amino acid composition or the food or beverage of the present invention is determined individually according to exercise, age, sex, body weight, eating condition, and the like. The amino acid composition of the present invention, the food, the beverage or the pharmaceutical product containing the same can be expected to improve the exercise performance by improving the muscle strength, so it is preferably used for people who do not have exercise habits, amateur athletes, professional athletes, and potential obesity. , obese, metabolic syndrome patients or animals, competition animals, etc.

The amino acid composition or the food or beverage of the present invention promotes the secretion of norepinephrine and imparts a sign to express an effective improvement in exercise performance. Preferably, sympathetic activation, concentration enhancement, fatigue prevention, fatigue improvement, and fatigue are expected. Reduced, fatigue-relieved, hyperglycemia, hyperlipidemia, lipolysis, improved lipid metabolism, improved motor performance, so you can also give signs to indicate, for example, improve muscle strength, increase instant power, improve endurance, and feel light. In the second half of the exercise, there is still physical strength, can maintain concentration, reduce physical fatigue, inhibit exhaustion, restrain tightness, can firmly train, can achieve ideal performance, move easily, and effectively surpass past performance limits.

Here, the labeling of the present invention is not particularly limited as long as it relates to an amino acid composition for promoting the secretion of norepinephrine, and the composition may be effectively labeled, advertised, etc., for example, for enhancing muscle strength. Amino acid composition, food, beverage, pharmaceutical container, packaging material or instructions. Further, the label of the present invention may be an advertisement for giving the composition an effective label, an advertisement or the like on a recording medium such as a leaflet, a booklet, an advertisement, a catalog, a poster, a book, a DVD, an electronic bulletin board, or the Internet. Furthermore, the content of the logo similar to the present invention may be reproduced and communicated to a third party via a verbal and/or recorded recording medium for the purpose of negotiating and/or facilitating the sale.

In the case of producing the amino acid composition, food, drink, or pharmaceutical for promoting the secretion of norepinephrine of the present invention, the above amino acid may be used and these may be mixed. Further, when used as a beverage, these may be mixed and dissolved in distilled water or the like. [Examples]

Hereinafter, the present invention will be more specifically described by showing examples, but the scope of the invention is not limited by the description of the examples.

Example 1: Preparation of Amino Acid Composition (Into Capsule) L-arginine, L-proline, and L-serine were filled into Cellulose White Capsule No. 00 (manufactured by Matsuya Co., Ltd.) to prepare a capsule. The amino acid composition (Example 1) was made to have 1800 mg of L-arginine, 1100 mg of L-proline, and 100 mg of L-serine. Further, L-arginine, L-valine and L-serine are manufactured by Kyowa Hakko Bio Co., Ltd.

Example 2: Exercise test A exercise test was carried out when the amino acid composition of the present invention was ingested. A summary of the test system is shown in Figure 1. As a test food, the test food (the encapsulated amino acid composition of Example 1) and the control food (in order to be unrecognizable from the appearance and maintain double-blind testability, in the same Cellulose White as the test food) Capsule No. 5 (manufactured by Matsuya Co., Ltd.) was added to a dextrin 3000 mg (Comparative Example 1).

The subjects (healthy persons aged 20 years and older (19 males)) were randomly divided into two groups, and the age and body weight at the time of screening were used as indicators, and a double-blind cross-comparison test was performed. In the first group (9 persons), the test food intake test was first performed, and then the control food intake test was performed at intervals of 6 days or longer, and the second group (10 persons) was subjected to the control food intake test in advance, and then at intervals of 6 days or more. Conduct a food intake test.

The test food was taken twice a day for 3000 mg each time for three days, and the exercise test was performed on the third day (Fig. 1). The dinner on the day before the exercise test was a designated diet with a fast period of 12.5 to 15.5 hours in the middle, and sugar jelly was taken on the exercise test day. The first time the ingested test food was taken, the bicycle dynamometer (Aero Bike (registered trademark) 75XLIII (manufactured by Konami Sports Life Co., Ltd.) was used 10 minutes after the first intake of the test food. Warm up, take the test food for 30 minutes and then carry out the following long-lasting exercise. In the long-lasting exercise, perform 45 minutes of exercise at 50% VO _{2 peak} intensity, with an interval of 15 minutes, and the first 50% VO _{2 peak} At the end of the intensity exercise, a second test of food intake is performed.

After 5 minutes from the end of the enduring exercise, a Wingate test was performed. The Wingate test is a representative method for assessing anaerobic exercise capacity. This experiment was carried out under conditions of a 7.5% weight load for 30 seconds of full pedaling. In addition, the above-mentioned exercises all use a bicycle dynamometer (warm-up, long-lasting exercise using Aero Bike (registered trademark) 75XLIII (manufactured by Konami Sports Life Co., Ltd.), and Wingate test using Power MaxVIII (manufactured by Konami Sports Life Co., Ltd.). )). On the day of the exercise test, six blood draws were performed (S1: before ingesting the jelly jelly, S2: when the long-term exercise started, S3: the first 50% VO _{2 peak} intensity exercise ended, S4: at the end of the persistent exercise, S5: Five minutes after the end of the Wingate test, S6: 20 minutes after the end of the Wingate test, blood levels in glucose metabolism/lipid metabolism were measured.

The concentration of adrenaline in the blood and the concentration of norepinephrine in the blood were measured by HPLC (HLC-725CAII, Tosoh Corporation). The measurement results of the concentration of adrenaline in the blood are shown in Fig. 2, and the measurement results of the concentration of norepinephrine in the blood are shown in Fig. 3. When Example 1 was ingested, the concentration of adrenaline in the blood and the concentration of norepinephrine in the blood increased during exercise as compared with the case of Comparative Example 1. In particular, the concentration of norepinephrine in the blood is between S3 (at the end of the first 50% VO _{2 peak} intensity exercise), S4 (at the end of the enduring exercise), and S5 (after 5 minutes after the end of the Wingate test). Change to a significantly high value.

When Example 1 was ingested, the tendency of the peak power in the Wingate test to become higher was observed as compared with the case of Comparative Example 1. A positive correlation was observed between the concentration of norepinephrine in the blood of S5 (5 minutes after the end of the Wingate test) and the Wingate test (Fig. 4). When Example 1 was ingested, the concentration of adrenaline in the blood and the concentration of norepinephrine in the blood became higher and the exercise performance was improved as compared with the case of ingesting Comparative Example 1.

Example 3: Preservability of Amino Acid Composition The 1800 mg of L-arginine, 1100 mg of L-proline, and 100 mg of L-serine were filled into Cellulose White Capsule No. 00 to prepare a capsule-containing amino acid composition, and filled. To the aluminum bag container. After storage for 2 weeks at room temperature, the amino acid-containing composition in the capsule remained undecomposed (reduced). [Industry Utilization]

The amino acid composition of the present invention has an effect of promoting the secretion of norepinephrine, has a tendency to promote the secretion of adrenaline, and can improve exercise performance or motivation for exercise. In this way, it can be designed to maintain and enhance the possibility that people with less exercise are accustomed to exercise, or the athletic performance of amateur athletes or professional athletes. At this time, it helps to prolong the healthy life of the Japanese. As a result, it can contribute to society by reducing medical expenses.

no.

Fig. 1 is an explanatory diagram showing an outline of a test system. Figure 2 is a graph showing the measured concentration of adrenaline in the blood. Figure 3 is a graph showing the measured concentration of norepinephrine in the blood. Figure 4 is a plot of the correlation between the concentration of norepinephrine in the blood and the peak power measured in Wingate after five minutes of the Wingate test.

Claims (9)

An amino acid composition for promoting the secretion of norepinephrine is composed of arginine, valine and serine. The amino acid composition according to claim 1, wherein the weight ratio of arginine, valine and serine is from 50 to 70:25 to 50:0.1 to 15. The amino acid composition according to claim 1, wherein the weight ratio of arginine, valine and serine is from 10 to 28:7 to 22:1. The amino acid composition according to any one of claims 1 to 3, which is contained in an edible container. A food, beverage or pharmaceutical for promoting the secretion of norepinephrine, comprising an effective amount of the amino acid composition according to any one of claims 1 to 4. The food, beverage or pharmaceutical product according to claim 5, which comprises a non-effective amount of an amino acid other than arginine, proline or serine. The food, beverage or pharmaceutical product according to claim 5 or 6, further comprising an active ingredient for promoting adrenaline and/or norepinephrine secretion by a non-amino acid. The food, drink, or pharmaceutical product according to any one of claims 5 to 7, which is contained in a container. An amino acid composition according to any one of claims 1 to 4, wherein the food, beverage or pharmaceutical product according to any one of claims 5 to 8 The manufacturing method comprises the steps of: mixing arginine, valine and serine.