TW201909759A - Amino acid composition for promoting muscle strength - Google Patents

Abstract

The subject of the present invention is to provide an amino acid composition having a new use of improving muscular strength based on a healthy non-fatigued state which has not been studied conventionally. The present invention relates to a muscular strength improving amino acid composition having arginine, valine and serine. Particularly, the present invention relates to an amino acid composition for improving muscular strength having a weight ratio of arginine, valine and serine of 50~70: 25~50: 0.1~15, and a food, a beverage or a medicine for improving muscular strength, comprising an amino acid composition in an effective amount.

Description

Amino acid composition for improving muscle strength

The present invention relates to an amino acid composition for enhancing muscle strength and to foods, beverages and pharmaceuticals comprising the amino acid composition.

It has been studied to improve exercise function or to recover fatigue accompanying exercise by taking up an amino acid composition. For example, Patent Document 1 discloses that the muscle exercise function is enhanced by ingesting a beverage containing a branched amino acid, and the amount of spontaneous exercise of the rat is improved (Example 6). Patent Document 2 describes a composition (VAAM) composed of an amino acid contained in saliva secreted by the hornet larvae, and is useful as a muscle strength agent or a fatigue recovery agent, and can be forced to swim by a mouse or the like. The effect was confirmed (Example 1 (1)). Patent Document 3 describes a physical strength enhancer comprising arginine as an essential component, and one or more components selected from the group consisting of glutamic acid, nucleotides, or nucleosides as an active ingredient. The effects were confirmed by weights such as rat swimming exercise (depletion experiment) or soleus muscle (Examples 6 and 7). These documents only study the persistence of muscle-sustainable exercise or have not studied muscle strength.

Patent Document 4 describes a muscle reinforcing agent whose active ingredient contains an amino acid composition composed of ornithine, citrulline, and arginine, although it is confirmed that a muscle enhancer promotes muscle protein synthesis and increases muscle mass in rats ( Experimental Examples 1 and 2), but only predictions regarding muscle strength increase (paragraph [0036]), were not confirmed.

Patent Document 5 describes an amino acid-containing composition for muscle fatigue recovery promotion comprising nine kinds of amino acids, and confirms the muscle strength after the eccentric contraction load of the rat for 6 hours, that is, the restored muscle strength (No. Seven figures). Further, Patent Document 6 describes a composition for inhibiting side effects such as reduction in muscle function by steroid using aspartic acid, leucine, and valine as an active ingredient, and confirms that the grip strength of the rat is suppressed (third) Figure). These documents attempt to restore muscle damage caused by exercise (Patent Document 5) or to suppress a decrease in muscle strength due to a disease (Patent Document 6). So far, no research has been conducted on the improvement of muscle strength with amino acid, especially on the basis of healthy and non-fatigue state. [Prior Technical Literature] [Patent Literature]

[Patent Document 1] Japanese Laid-Open Patent Publication No. Hei. No. Hei. No. Hei. No. Hei. No. Hei. 5] International Publication No. 2013/021591 [Patent Document 6] International Publication No. 2008/072663

[Problems to be Solved by the Invention] Accordingly, an object of the present invention is to provide an amino acid composition which has a novel use, that is, has not been studied in the past, and has improved muscle strength based on a state of being healthy and not fatigued. [Means for solving problems]

In order to solve the aforementioned problems, the inventors of the present invention focused on arginine, lysine, and serine in an effort to find a high muscle strength improving effect in an amino acid composition containing only these three amino acids. As a result of conducting the research, the present invention has been completed.

That is, the present invention relates to the following invention. [1] An amino acid composition for improving muscle strength, which is composed of arginine, valine and serine. [2] The amino acid composition according to the above [1], wherein the weight ratio of arginine, valine and serine is from 50 to 70:25 to 50:0.1 to 15. [3] The amino acid composition according to the above [1], wherein the weight ratio of arginine, valine and serine is from 10 to 28:7 to 22:1. [4] The amino acid composition according to any one of [1] to [3] above which is contained in an edible container. [5] A food, a beverage, or a pharmaceutical composition for improving muscle strength, comprising an effective amount of the amino acid composition according to any one of the above [1] to [4]. [6] The food, drink or pharmaceutical according to the above [5], which comprises a non-effective amount of an amino acid other than arginine, proline or serine. [7] The food, drink or pharmaceutical according to the above [5] or [6], which further comprises an active ingredient for improving muscle strength of a non-amino acid. [8] The food, drink or pharmaceutical according to any one of the above [5] to [7], which is contained in a container. The method for producing a food, beverage, or pharmaceutical according to any one of the above [1] to [4], wherein the food, beverage, or pharmaceutical product according to any one of the above [5] to [8] Contains: mixed arginine, valine and serine. [Effect of the invention]

According to the present invention, there is provided an amino acid composition which can improve muscle strength, particularly muscle strength based on a healthy and non-fatigue state, a food, a drink, and a pharmaceutical containing the amino acid composition. In particular, according to the present invention, the instantaneous muscle strength is increased, the muscle force mechanical measurement value (for example, the grip strength or the leg stretching muscle strength, etc.) is increased, and the exercise performance is improved.

The amino acid composition for improving muscle strength of the present invention is composed of three types of arginine, lysine and serine. The weight ratio of arginine, valine and serine is 50 to 70:25 to 50:0.1 to 15 in one aspect, and 10 to 28:7 to 22:1 in other aspects. It is preferably 15 to 18:11 to 14:1. By doing this weight ratio, the muscle strength improvement effect is further achieved.

The amino acid composition of the present invention, in one aspect, is contained in a container, preferably in an edible container. The edible container refers to a capsule for formulation and the like. The amino acid composition contained in the container can be directly added to foods, beverages, and pharmaceuticals by using an edible container.

The amino acid composition of the present invention has an effect of improving muscle strength, and can be used in an effective amount in a food, a drink or a pharmaceutical. A food or beverage to which an effective amount of an amino acid composition is added becomes a composition different from a food or beverage directly existing in nature. The effective amount as such may be appropriately determined by those skilled in the art, for example, the total weight of arginine, valine and serine is from 500 mg to 10,000 mg, preferably from 1500 mg to 5000 mg, particularly preferably from 2500 mg to 3500 mg.

The food, drink or pharmaceutical of the present invention may also contain an amino acid other than arginine, lysine or serine. In the present invention, an amino acid other than arginine, lysine, and serine may be used to increase muscle strength, and may also contain, for example, an amount that is not effective for achieving a muscle-boosting effect (non-effective amount). . An ineffective amount like this can be appropriately determined by those skilled in the art.

The amino acid used in the present invention may also be any one of an L-form, a D-form, and a DL-form, and is preferably an L-form, a DL-form, or more preferably an L-form. Further, the amino acids used in the present invention may each be not only a free form but also an amino acid salt. The amino acid salt is not particularly limited as long as it is an amino acid salt which can be tolerated as a food, a drink or a pharmaceutical, and examples thereof include an acid addition salt of an amino acid or a salt with a base.

As the salt of the food which can be tolerated as the amino acid used in the present invention, as the acid forming the salt, for example, inorganic acids such as hydrochloric acid, hydrogen bromide, sulfuric acid, phosphoric acid, etc.; acetic acid, lactic acid, citric acid, tartaric acid , organic acids such as maleic acid, fumaric acid, and monomethyl sulfuric acid. Further, examples of the base which can be used as a salt of a food include an inorganic base such as sodium, potassium, calcium or ammonia, or ethylenediamine, propylenediamine, ethanolamine, monoalkylethanolamine, dialkylethanolamine or diethanolamine. Organic bases such as triethanolamine. The amino acid salt used in the present invention may also be a hydrate (hydrated salt), and as such a hydrate, for example, a hydrate of 1 to 6 is exemplified.

The amino acid used in the present invention may be any one obtained by extracting and purifying animals, plants, microorganisms containing these, or by chemical synthesis, fermentation, or genetic recombination. Also, it is also commercially available. The amino acid used in the present invention is preferably one which is used alone in high purity. For example, an amino acid having a purity equal to or higher than that specified in the "Japanese Food Additives" is used. Further, those having a physiologically acceptable salt can also be used as these amino acids.

In the production of the amino acid composition of the present invention, the above amino acid may also be mixed. Further, when the solution is produced, it may be mixed and dissolved with distilled water, ethanol or the like. The food, drink or pharmaceutical of the present invention may further comprise an active ingredient for muscle strength enhancement of a non-amino acid. As such an active ingredient, for example, protein or vitamin D is mentioned.

The ingredient which can be contained in the food, drink or pharmaceutical of the present invention is not particularly limited as long as it can be ingested (administered) into the body, but is usually a component mixed with a pharmaceutical product and/or a food or drink, and can be blended, for example. Vitamin B1, vitamin B2, vitamin B6, pantothenic acid and other vitamins, coenzyme Q10, carnitine, alpha-lipoic acid and other vitamins, iron, zinc and other minerals, glucose, fructose, galactose, mannose, sucrose, maltose , sugars such as lactose, organic acids such as citric acid, tartaric acid, ascorbic acid, malic acid, acetic acid, oxalic acid, phosphoric acid, lactic acid, butyric acid, succinic acid, fumaric acid and gluconic acid, caffeine, theobromine, theanine, Polyphenols such as catechins.

Further, an amino acid component such as sodium glutamate or taurine, sucralose, neotame, ethyl sulfamate, thaumatin, stevia extract, licorice extract, mangosteen extract, saccharin, sodium saccharin, and the like may be mixed. Sweeteners such as glycyrrhizic acid, disodium glycyrrhizinate, trisodium glycyrrhizinate, dihydrochalcone, stevioside, stevioside A, minerals such as zinc, selenium, iron, copper, trehalose, oligosaccharides, rare Sugars such as sugar, lactic acid bacteria, Lactobacillus brevis, Propionibacterium, and other useful microorganisms or cultures thereof, and existing functional materials such as royal jelly, collagen, ceramide, glucosamine, reduced astaxanthin, polyphenols, and the like. , pH adjusters, excipients, acidifiers such as citric acid, colorants, emulsifiers, preservatives, and the like.

The method for ingesting (administering) the amino acid composition of the present invention is not limited as long as it can be ingested (administered) into the body, but is preferably a tablet, a coated tablet, a capsule, or a granule. , powder, solution, syrup, emulsion, etc. are administered orally. These various preparations can be used in the field of pharmaceutical preparations such as excipients, binders, disintegrants, lubricants, coloring agents, flavoring agents, solubilizing agents, suspending agents, coating agents, etc. according to a conventional method, and conventionally used auxiliary agents can be used. Formulated in the amino acid composition of the present invention.

In one aspect, the beverage food of the present invention may be used as a food and beverage (including a health food, a functional food, a nutritious food, and the like, and may be provided as a food and drink, etc.). Nutritional supplements, other health foods, supplements).

The beverage food of the present invention can be used as a dairy product such as a beverage or a yogurt drink, a jelly product, an ice cream, an ice product, a biscuit, a mouth gel product, a powder product, a granulated product, a sheet product, a capsule product. , solid products such as ingot products, candy, etc.

The food or drink of the present invention is not particularly limited as long as it is a solution, a suspension, an emulsion, a powder, a solid molded product, or the like, which can be orally ingested. Specifically, for example, dairy products such as milk beverages, yogurt drinks, lactic acid bacteria beverages, fermented milk, ice creams, creams, and cheeses; refreshing drinks, juice drinks, vegetable drinks, soy milk drinks, coffee drinks, and tea drinks are mentioned. , jelly drinks, cocoa, smoothies and other powder drinks or sports powder drinks, nutritionally enhanced powder drinks, powdered foods, powder soups, taro, concentrated drinks, alcoholic beverages, etc.; bread, pasta, noodles, cake powder, Wheat flour products such as fried flour and bread flour; desserts such as chocolate, chewing gum, candy, biscuits, soft candy, snacks, Japanese desserts, jellies, puddings, etc.; desserts such as processed seasonings, flavor seasonings, and comprehensive seasonings; Curry, curry, pasta sauce, fire pot, stew, Japanese food, sterilized bagged food; processed oils, butter, margarine, spreads, cannabis and other oils; freeze-dried foods and other fast-food foods; Agricultural canned food, jam, peel sauce, cooked beans, oysters, vegetable porridge and other agricultural products; aquatic products; processed products; Baked rice, frozen food macaroni gratin, home cooking, frying and the like; liquid diet, food thickening adjustment, as well as animal feed, troches and the like.

When the amino acid composition of the present invention is used in a food or drink, the other ingredients are not particularly limited, but the aforementioned foods and drinks may use water, protein, carbohydrates, lipids, vitamins, minerals, organic acids, and organic bases. Juices, flavorings, etc. are used as ingredients. The protein is exemplified by whole milk powder, skimmed milk powder, partially skimmed milk powder, casein, whey powder, whey protein, whey protein concentrate, whey protein isolate, α-casein, β-casein, kappa - casein, beta-lactoglobulin, alpha-lactalbumin, lactoferrin, soy protein, egg protein, meat protein, hydrolysates of these; butter, whey minerals, butter, whey, non-protein nitrogen, Various milk-derived components such as sialic acid, phospholipids, and lactose. Carbohydrates include sugars, processed starches (except dextrin, soluble starch, British starch, oxidized starch, starch esters, starch ethers, etc.), dietary fiber, and the like. As the lipid, for example, lard oil, fish oil, and the like, animal fat such as fractionated oil, hydrogenated oil, and transesterified oil; palm oil, safflower oil, corn oil, rapeseed oil, coconut oil, fractionated oil of these, hydrogenation Vegetable oils such as oil and transesterified oil. As vitamins, for example, vitamin A, carotene, vitamin B group, vitamin C, vitamin D group, vitamin E, vitamin K group, vitamin P, vitamin Q, nicotinic acid, pantothenic acid, biotin, inositol, Choline, folic acid, etc., as minerals, for example, calcium, potassium, magnesium, sodium, copper, iron, manganese, zinc, selenium, and the like. As the organic acid, for example, malic acid, citric acid, lactic acid, tartaric acid or the like is mentioned. These components may be used in combination of two or more kinds, and foods containing many synthetic products and/or these may also be used.

The food, the beverage, and the pharmaceutical product of the present invention may be contained in a container, and the container may be selected from the group consisting of plastic, paper, metal, ceramic, or composite materials thereof. It can be used as a loading container in these containers. That is, a food and beverage packaged in a container containing the amino acid composition of the present invention, and a pharmaceutical product filled in the container can be exemplified. Further, the container may be coated with a shrink film made of plastic, a light-shielding film (for example, a metal foil film, a black or dark ink coating film), or two or more types of the container or the film may be used in combination. From the viewpoint of deterioration of flavor and decomposition (reduction), a container or film having a light-shielding property is preferable.

The amino acid composition or the food or beverage of the present invention is composed of a unit package form per meal, and includes arginine, lysine, and serine in the unit package form. Here, the "unit packaging form per meal" refers to a form in which the amount of intake per meal is determined in advance, for example, as a foodstuff that can be orally ingested in a specific amount, not only a general food but also a beverage (such as a beverage). , health supplement foods, health functional foods, supplies, etc. In the case of "unit packaging form per meal", for example, in the case of a liquid beverage, a gel, a paste or a paste jelly, a powder, a granule, a capsule, or a solid food, Packaging containers such as metal cans, glass bottles (bottles, etc.), plastic containers (PET bottles, etc.), packaging, bags, film containers, cartons, etc., to specify a specific amount (amount), or by ingesting each meal The amount (usage, amount) indicates a form in which a specific amount is specified in a packaging container or a web page.

The daily intake of the amino acid composition or the food or beverage of the present invention is determined individually according to exercise, age, sex, body weight, eating condition, and the like. The amino acid composition of the present invention, the food, the beverage or the pharmaceutical product containing the same can be expected to improve the exercise performance by improving the muscle strength, so it is preferably used for people who do not have exercise habits, amateur athletes, professional athletes, and potential obesity. , obese, metabolic syndrome patients or animals, competition animals, etc.

The amino acid composition or the food or beverage of the present invention is labeled to express effective muscle strength to exercise performance, and it is preferable to increase muscle strength, increase instantaneous force, and improve exercise performance, so that a mark can be given to indicate, for example. It can exert high muscle strength, can achieve ideal performance, move easily, and effectively exceed the performance limits of the past.

Here, the labeling of the present invention is not particularly limited as long as it relates to an amino acid composition for improving muscle strength, and the composition may be effectively labeled, advertised, etc., for example, containing an amine group for enhancing muscle strength. Acid composition, food, beverage, pharmaceutical container, packaging material or instructions. Further, the label of the present invention may be an advertisement for giving the composition an effective label, an advertisement or the like on a recording medium such as a leaflet, a booklet, an advertisement, a catalog, a poster, a book, a DVD, an electronic bulletin board, or the Internet. Furthermore, the content of the logo similar to the present invention may be reproduced and communicated to a third party via a verbal and/or recorded recording medium for the purpose of negotiating and/or facilitating the sale.

When the amino acid composition for improving muscle strength of the present invention, a food, a drink, or a pharmaceutical is produced, these amino acids may be used and these may be mixed. Further, when used as a beverage, these may be mixed and dissolved in distilled water or the like. [Examples]

Hereinafter, the present invention will be more specifically described by showing examples, but the scope of the invention is not limited by the description of the examples.

Example 1: Preparation of Amino Acid Composition (Into Capsule) L-arginine, L-proline, and L-serine were filled into Cellulose White Capsule No. 00 (manufactured by Matsuya Co., Ltd.) to prepare a capsule. The amino acid composition (Example 1) was made to have 1800 mg of L-arginine, 1100 mg of L-proline, and 100 mg of L-serine. Further, L-arginine, L-valine and L-serine are manufactured by Kyowa Hakko Bio Co., Ltd.

Example 2: Muscle strength measurement experiment The muscle strength at the time of ingesting the amino acid composition of the present invention was measured. A summary of the test system is shown in Figure 1. As a test food, the test food (the encapsulated amino acid composition of Example 1) and the control food (in order to be unrecognizable from the appearance and maintain double-blind testability, in the same Cellulose White as the test food) Capsule No. 5 (manufactured by Matsuya Co., Ltd.) was added to a dextrin 3000 mg (Comparative Example 1).

The subjects (healthy persons aged 20 years and older (19 males)) were randomly divided into two groups, and the age and body weight at the time of screening were used as indicators, and a double-blind cross-comparison test was performed. In the first group (9 persons), the test food intake test was first performed, and then the control food intake test was performed at intervals of 6 days or longer, and the second group (10 persons) was subjected to the control food intake test in advance, and then at intervals of 6 days or more. Conduct a food intake test.

The test food was taken twice a day and 2 days before the measurement of muscle strength, and once a day on the day of muscle strength measurement. The dinner on the first day of the muscle strength measurement is the designated diet, with a fasting period of 12.5 to 15.5 hours in the middle, and the measurement of muscle strength on the day of intake of sugar jelly. After taking the icing sugar for 1 hour, the test food was ingested, and after taking the test food for 10 minutes, the bicycle was measured by a bicycle dynamometer (Aero Bike (registered trademark) 75XLIII (manufactured by Konami Sports Life Co., Ltd.), and the test food was taken. The muscle strength measurement was performed after 30 minutes.

Muscle force measurement is the measurement of grip strength and constant-speed leg extension muscle strength. The grip strength was measured by a digital grip meter "GRIP D" (manufactured by Takei Instrumentation Industry Co., Ltd.), and the average value of the grip strength of both hands was calculated. The constant-speed leg stretching muscle strength was measured by "LEG POWER" (manufactured by Takei Instrumentation Industry Co., Ltd.) to measure the muscle strength when the curved legs were stretched in one breath. The isometric leg stretching muscle strength was measured three times in succession, using the maximum value. The corresponding one-sample t-test was used to verify the difference in the mean. The level of significance is set to 5%.

The measurement result of the grip strength is shown in Fig. 2, and the measurement result of the isometric leg extension muscle strength is shown in Fig. 3. When Example 1 was ingested, both the grip strength and the constant-speed leg stretching muscle strength were improved as compared with the ingestion of Comparative Example 1. This result indicates that the amino acid composition of the present invention enhances muscle strength, particularly muscle strength based on a healthy and non-fatigue state, and both hands and feet are elevated.

Example 3: Preservability of Amino Acid Composition The 1800 mg of L-arginine, 1100 mg of L-proline, and 100 mg of L-serine were filled into Cellulose White Capsule No. 00 to prepare a capsule-containing amino acid composition, and filled. To the aluminum bag container. After storage for 2 weeks at room temperature, the amino acid-containing composition in the capsule remained undecomposed (reduced). [Industry Utilization]

The amino acid composition of the present invention enhances athletic performance or motivation for exercise by enhancing the muscle strength at the beginning of exercise. In this way, it can be designed to maintain and enhance the possibility that people with less exercise are accustomed to exercise, or the athletic performance of amateur athletes or professional athletes. At this time, it helps to prolong the healthy life of the Japanese. As a result, it can contribute to society by reducing medical expenses.

no.

Fig. 1 is an explanatory diagram showing an outline of a test system. Fig. 2 is a view showing measurement results of grip strength in the case of ingesting Example 1 and Comparative Example 1, respectively. Fig. 3 is a graph showing the measurement results of the isosceles leg muscle strength in the case of ingesting Example 1 and Comparative Example 1, respectively.

Claims (9)

An amino acid composition for improving muscle strength is composed of arginine, lysine and serine. The amino acid composition according to claim 1, wherein the weight ratio of arginine, valine and serine is from 50 to 70:25 to 50:0.1 to 15. The amino acid composition according to claim 1, wherein the weight ratio of arginine, valine and serine is from 10 to 28:7 to 22:1. The amino acid composition according to any one of claims 1 to 3, which is contained in an edible container. A food, a beverage, or a pharmaceutical product for improving muscle strength, comprising an effective amount of the amino acid composition according to any one of claims 1 to 4. The food, beverage or pharmaceutical product according to claim 5, which comprises a non-effective amount of an amino acid other than arginine, proline or serine. The food, beverage or pharmaceutical product according to Item 5 or Item 6 of the patent application, further comprising an active ingredient for improving muscle strength of a non-amino acid. The food, drink, or pharmaceutical product according to any one of claims 5 to 7, which is contained in a container. An amino acid composition according to any one of claims 1 to 4, wherein the food, beverage or pharmaceutical product according to any one of claims 5 to 8 The manufacturing method comprises the steps of: mixing arginine, valine and serine.