TW201831672A - Cell storage container allowing a cell sample to be in a confined space during the sampling process so as to reduce the opportunities of polluting the sample - Google Patents

Abstract

A cell storage container comprises: a storage tube having an open end and an accommodating space, wherein the open end is provided with a connection interface; a plug member, wherein one end of the plug member is tightly assembled to the connection interface, and the other end of the plug member protrudes from the storage tube and forms a pin portion; a cover body correspondingly disposed on the open end, a through hole is arranged on one side of the cover body, and the pin portion penetrates through the through hole; and a plug cover detachably disposed on the cover body. The present invention sequentially arranges the plug member, the cover body and the plug cover through the storage tube, so that the accommodating space is in a tightly sealed condition. The plug cover is detachable from the cover body, which makes it easy for a user to insert the pin, so that the cell sample is in a confined space during the sampling process, reducing the opportunities of being polluted.

Description

Cell storage container

The invention relates to a storage container, and in particular to a cell storage container.

According to the blood storage technology, it is a very important advancement in the history of human medical treatment. The advancement of blood storage technology has made it possible to treat many major diseases and diseases, and thus prolong human life.

However, in recent years, research on specific cells in the blood has become more and more intensive. For example, specific white blood cells in the blood have been isolated to study the related issues of immunity, and the use of stem cells has been most noticed. Therefore, in addition to conventional blood storage, the storage of specific cells and stem cells in the blood has received increasing attention.

In addition, because many people in today's society want to prevent hereditary diseases, they will go to the medical institutions for stem cell screening. After medical sampling and testing, personal stem cell data will be stored in medical institutions in paper records or stored in data. In the computer equipment of the medical institution.

Moreover, stem cells are cells that have the potential for self-regeneration and infinite differentiation in the early stages of development of higher organisms. Although adult species still retain specific types of stem cells, their numbers and differentiation ability are not as early as the development of stem cells, which makes the early stage of stem cells more precious, and its storage method has become a very important part of the research. . Taking cord blood as an example, cord blood is the most convenient way to obtain human stem cells. However, people only have one chance to get their own cord blood in their lifetime. Therefore, the storage of cord blood must be properly planned to meet the needs of future inspections and possible use.

With regard to blood samples, they usually need to be stored at low temperatures, such as liquid nitrogen (-196 ° C), and in the future, it is often necessary to obtain a small amount of blood for testing. Among them, if it is not convenient to obtain a small amount of blood, the blood sample in the whole storage may be thawed and destroyed. Therefore, at least the following points should be noted for the storage of blood samples: first, sterile space; second, in batches; third, convenience of access.

Furthermore, the conventional cell storage container often has only one storage tube and a cover, and the cover is screwed to the storage tube, and when the cell storage container is taken out in a liquid nitrogen tank, and the user pre-extracts When the cells in the tube are stored, at this time, the user must directly insert the top of the cover with a syringe, and then the cells can be extracted; however, when the cell storage container is in the state of extracting cells, the user must use A large force can break the cover or open the cover, causing inconvenience in use and contamination of cells in the storage tube.

Accordingly, in order to effectively research and utilize precious stem cells, there is an urgent need for a cell storage container that is more stable and convenient to store.

The main object of the present invention is to provide a cell storage container which enables the self-sampling process of a cell sample to be in a confined space, reducing the chance of contamination.

A secondary object of the present invention is to provide a cell storage container which is easy to assemble, can quickly take out cells, and can directly collect cells in a closed container without opening and separating an upper cover of the cell storage container. Keeping the sterility of the cells, at the same time, omitting the complicated procedures in the existing sampling, saving the intermediate link time, and having the characteristics of convenient use, safety and reliability.

Still another object of the present invention is to provide a cell storage container which is simple in structure, stable, and suitable for industrial production, and which is capable of rapidly providing sufficient experimentally sufficient cellular material for subsequent experiments.

In order to achieve the above objective, the present invention provides a cell storage container comprising: a storage tube having an open end and a closed end, and the storage tube has an accommodating space therein, and the open end has a set of interfaces, the set of interfaces is in communication with the accommodating space; a plug member having a plurality of connecting portions and a pin portion, the connecting portions are closely assembled to the group of interfaces, and the device is closed a space is formed, and the pin portion protrudes from the storage tube; a cover body is correspondingly disposed at the open end, and one side of the cover body is provided with a through hole, and the pin portion is The through hole is disposed through the through hole; and a plug cover is detachably assembled to the cover body, and the plug cover closes the through hole.

Preferably, the outer side of the open end of the storage tube is provided with an external thread, and the inner side of the cover body is provided with an internal thread corresponding to the external thread, and the internal thread can be screwed to the external thread for the A cover body is disposed at the open end.

Preferably, the outer side of the open end of the storage tube is surrounded by a protrusion, and one side of the protrusion is provided with a plurality of ratchet teeth, and the cover system is provided with a plurality of reverse teeth corresponding to the ratchet. The opposite reverse teeth are correspondingly engaged with the ratchets for the cover to be locked against the open end of the storage tube.

Preferably, the plug member is made of a silicone material, and a peripheral portion of the plug cover is circumferentially provided with a dial block, and the set portion is recessed with a groove, and the inner side of the groove is circumferentially The system tapers toward the bottom of the groove.

Preferably, the plug is provided with a convex portion between the set portion and the pin portion, the convex portion is abutted against a peripheral edge of one of the open ends, and the cover system has a set of joints. The groove is formed by the bottom of the groove of the set of grooves, and the bottom of the groove of the set of grooves is pressed against the convex portion.

Preferably, a protrusion is formed on one side of the cover body, the protrusion is through the through hole, and the outer circumference side of the protrusion is circumferentially provided with at least one flange, and the cover is The inner peripheral side is provided with a card slot corresponding to the flange, and the flange is correspondingly engaged with the card slot for the plug cover to be engaged with the boss.

Preferably, the outer peripheral side of the plug cover is provided with a dial block, and the bottom side of the plug cover is provided with a slope toward the inner circumference side, and the slope is used for the quick cover of the plug cover. Connected to the stud and engage the flange in the card slot.

Preferably, a peripheral portion of the closed end of the storage tube is provided with a standing portion so as to be convex downward.

Preferably, the accommodating space is formed in an inverted arc shape adjacent to the closed end.

Preferably, the outer peripheral side of the cover is surrounded by a plurality of protruding ribs.

According to the cell storage container provided by the present invention, the user can first take out non-embryonic stem cells from the bone marrow, fat, dermis and umbilical cord blood of the human body, and then perform cell culture, and then culture the cells. The cells are collected into the cell storage container, and finally the cell storage container is placed in a liquid nitrogen tank for rapid freezing; in contrast, the present invention mainly comprises the plug member and the cover sequentially disposed through the open end of the storage tube. And the plug cover, so that the accommodating space is completely sealed, and can be used for closing and conserving cells, and the plug cap can be separated into the cover body, so that the pin portion is exposed, and the accommodating portion is convenient The user inserts the needle to remove the cells.

Therefore, the present invention has the advantages of simple assembly, rapid injection or removal of cells, and the cells can be directly collected in a closed container without opening the lid of the cell storage container, thereby maintaining the sterility of the cells, and omitting the The existing complicated program during sampling saves the intermediate link time and is easy to use, safe and reliable.

Referring to FIG. 1 and FIG. 2, together with FIG. 3, a perspective view, an exploded perspective view and a cross-sectional view of a preferred embodiment of the present invention are disclosed. A cell storage container 100 is disclosed. :

A storage tube 10 having an open end 11 and a closed end 12, and the storage tube 10 has an accommodating space 13 therein, and the accommodating space 13 is formed to be rounded adjacent to the closed end 12 The open end 11 has a set of interfaces 14 that communicate with the accommodating space 13. In the present embodiment, an outer thread 15 is disposed on the outer side of the open end 11 of the storage tube 10. Further, a convex portion 16 is disposed on the outer side of the open end 11 of the storage tube 10, and the convex portion 16 is located outside the outer end of the open end 11 of the storage tube 10. The lower side of the thread 15 is provided with a plurality of ratchet teeth 161 on one side of the projection 16; and a standing portion 17 is formed on the periphery of the closed end 12 of the storage tube 10 so as to be convex downward.

A plug member 20 having a plurality of connecting portions 21 and a pin portion 22, the connecting portion 21 is tightly assembled to the set of interfaces 14 and enclosing the receiving space 13, and the pin portion 22 is closed. It protrudes from the storage tube 10. In the embodiment, the plug member 20 is made of a silicone material, and the connecting portion 21 is recessed with a recess 211. The inner side of the recess 211 is tapered toward the bottom of the slot. A convex portion 23 is disposed between the connecting portion 21 and the pin portion 22, and when the connecting portion 21 is assembled to the set of interfaces 14, the convex portion 23 abuts against the open end 11 One side of the circumference.

A cover body 30 is disposed on the open end 11 , and a through hole 31 is formed in one side of the cover body 30 , and the pin portion 22 is disposed through the through hole 31 . In the embodiment, the outer peripheral side of the cover body 30 is spaced around the protruding plurality of ribs 32, and the inner side of the cover body 30 is provided with an internal thread 33 corresponding to the external thread 15, and the internal thread 33 can be screwed. a thread 15 is disposed outside the storage tube 10 for the cover 30 to be disposed at the open end 11; further, the cover 30 is provided with a plurality of reverse teeth 35 corresponding to the ratchet teeth 161, and the reverse The latching teeth 35 are correspondingly engaged with the ratchet teeth 161, so that the cover body 30 is locked against the open end 11 of the storage tube 10, and a protrusion 34 is protruded from the top side of the cover body 30. The through-hole 31 is formed in the protruding post 34, and the outer peripheral side of the stud 34 is circumferentially provided with at least one flange 341. The cover 30 has a plurality of slots 36, and the set of slots 36 is provided. The through hole 31 is formed through the bottom of the groove, and when the cover 30 is assembled to the open end 11, the groove bottom of the set of grooves 36 is pressed against the convex portion 23.

A plug cover 40 is detachably assembled to the cover body 30, and the plug cover 40 closes the through hole 31. In the embodiment, the outer peripheral side of the plug cover 40 is surrounded by a dial 41, and the inner peripheral side of the plug cover 40 is recessed with a slot 42 corresponding to the flange 341, and the flange 341 is provided. The slanting surface 43 is connected to the inner peripheral side of the bottom side of the plug cover 40, and the inclined surface 43 is configured for the plug cover 40 to be quickly assembled to the convex portion. The post 34 is engaged with the flange 341.

For a further understanding of the structural features of the present invention, the application of the technical means, and the intended effect, the manner of use of the present invention will be described. It is believed that the present invention may be more deeply and specifically understood as follows:

Please refer to FIG. 3, which is a cross-sectional view and a schematic view of a preferred embodiment of the present invention, as shown in FIG. 4 and FIG. Since the user can first take out non-embryonic stem cells from the bone marrow, fat, dermis, and umbilical cord blood of the human body in the state of use, and then perform cell culture, and then collect the cultured cells into the cell storage container 100. Finally, the cell storage container 100 is placed in a liquid nitrogen tank for rapid freezing; in contrast, the present invention mainly includes the plug member 20, the cover body 30, and the same through the open end 11 of the storage tube 10. The cap 40 is configured to completely seal the accommodating space 13 and can be used for closing the cells, and the cap 40 can be detached from the cap 30 to expose the pin portion 22, It is convenient for the user to take out the cells through the syringe 200.

Therefore, the present invention has the advantages of simple assembly, quick removal of cells, and the need to open the lid 30 separating the cell storage container 100, thereby collecting cells directly in the closed container, thereby maintaining the sterility of the cells, and omitting the The existing complicated program during sampling saves the intermediate link time and is easy to use, safe and reliable.

Please refer to FIG. 2 and FIG. 3 together, which is an exploded perspective view and a cross-sectional view of a preferred embodiment of the present invention. First, from the structure shown in FIG. 2 and FIG. 3, it can be seen that the bump 16 of the storage tube 10 of the present invention is provided with a plurality of ratchet teeth 161, and the cover body 30 is provided with a plurality of corresponding ratchet teeth 161. The reverse latching teeth 35 are correspondingly engaged with the ratchet teeth 161, so that the cover body 30 is locked against the open end 11 of the storage tube 10, and the cover body 30 can be prevented. The storage tube 10 is easily separated by an external force, and more specifically, the cover 30 is stably connected to the storage tube 10. The second embodiment of the present invention further includes the dial 41 surrounded by the outer peripheral side of the plug cover 40. In the state of use, the user uses the hand to indicate the force to dial the dial 41 and force the plug cover 40 to The card slot 42 separates the flange 341 so that the cover 40 is separated from the cover 30 for the user to pin the pin. Furthermore, the present invention is provided with the standing portion 17 surrounded by the closed end 12 of the storage tube 10, and the standing portion 17 can provide a user to firmly erect the storage tube 10 on a plane.

It is worth mentioning that the accommodating space 13 is in the shape of an inverted arc cone near the bottom of the closed end 12, and the inverted arc-shaped structure can facilitate the user to quickly extract the needle through the needle 200. The cells at the bottom of the space 13 are accommodated.

It is worth mentioning that, since the plug member 20 is made of a silicone material, and the inner circumference of the groove 211 of the plug member 20 is tapered toward the bottom of the groove, please refer to FIG. The thickness of the side sill at the notch of the groove 211 is smaller than the thickness of the side sill of the bottom of the groove. More specifically, the side sill at the notch of the groove 211 has better deformability and flexibility, so When the plug member 20 is assembled, the user can quickly assemble the plug member 20 to the set of interfaces 14. At the same time, the plug member 20 can securely close the set of interfaces 14 so that the accommodating space 13 is completely sealed.

Hereafter, the features of the present invention and its achievable efficacy are stated as follows:

The cell storage container 100 of the present invention is mainly provided with the plug member 20, the cover body 30 and the plug cover 40 through the open end 11 of the storage tube 10, so that the accommodating space 13 is completely completed. The sealing state can be used to close the storage cells, and the cap 40 can be separated into the cover 30 so that the pin portion 22 is exposed, so that the user can insert the needle. In this way, the creative system has the following technical effects:

First, the present invention enables the self-sampling process of the cell sample to be in a confined space, reducing the chance of contamination.

Secondly, the present invention has the advantages of simple assembly, quick removal of cells, and the need to open the cover 30 separating the cell storage container 100, thereby collecting cells directly in the closed container, thereby maintaining the sterility of the cells, and omitting the existing The complicated program during sampling saves the intermediate link time and is easy to use, safe and reliable.

Third, the invention has the advantages of simple structure, firmness, and is suitable for industrial production, and the company can quickly provide sufficient experimental cell materials for subsequent experiments.

In summary, the present invention has excellent advancement and practicability in similar products, and at the same time, the technical materials of such structures are frequently investigated at home and abroad, and the same structure is not found in the literature. The invention already has the invention patent requirements, and the application is filed according to law.

However, the above-mentioned embodiments are merely preferred embodiments of the present invention, and the equivalent structural changes of the present invention and the scope of the patent application are intended to be included in the scope of the present invention.

100‧‧‧ cell storage container

10‧‧‧ storage tube

11‧‧‧Open end

12‧‧‧closed end

13‧‧‧ accommodating space

14‧‧‧ group interface

15‧‧‧ external thread

16‧‧‧Bumps

161‧‧‧ ratchet

17‧‧‧Standing Department

20‧‧‧plugs

21‧‧‧Team

211‧‧‧ Groove

22‧‧‧ Pin section

23‧‧‧ convex

30‧‧‧ cover

31‧‧‧through hole

32‧‧ ‧ ribs

33‧‧‧ internal thread

34‧‧‧Bump

341‧‧‧Flange

35‧‧‧Reverse card teeth

36‧‧‧ group slot

40‧‧‧ 塞盖

41‧‧ ‧ dial

42‧‧‧ card slot

43‧‧‧Bevel

200‧‧‧Syringe

BRIEF DESCRIPTION OF THE DRAWINGS Figure 1 is a perspective view of a preferred embodiment of the present invention. Figure 2 is an exploded perspective view of a preferred embodiment of the present invention. Figure 3 is a cross-sectional view of a preferred embodiment of the present invention. Figure 4 is a schematic illustration of the use of a preferred embodiment of the present invention. Figure 5 is a schematic illustration of the use of a preferred embodiment of the present invention.

Claims (10)

A cell storage container comprising: a storage tube having an open end and a closed end, wherein the storage tube has an accommodating space, and the open end has a set of interfaces, the set of interfaces Connected to the accommodating space; a plug member having a plurality of joint portions and a pin portion, the assembly portion is tightly assembled to the set of interfaces, and the accommodating space is closed, and the pin is closed The cover is protruded from the storage tube; a cover body is correspondingly disposed at the open end, and one side of the cover body is provided with a through hole, and the pin portion is disposed through the through hole; And a plug cover that is detachably assembled to the cover body, and the plug cover closes the through hole. The cell storage container according to claim 1, wherein the outer side of the open end of the storage tube is provided with an external thread, and the inner side of the cover body is provided with an internal thread corresponding to the external thread, and the internal thread is The external thread can be screwed to the cover body at the open end. The cell storage container according to the first aspect of the invention, wherein the outer side of the open end of the storage tube is surrounded by a protrusion, and one side of the protrusion is provided with a plurality of ratchets, and the cover system corresponds to The ratchet is provided with a plurality of reverse teeth, and the reverse teeth are correspondingly engaged with the ratchets for the cover to be locked against the open end of the storage tube. The cell storage container according to claim 1, wherein the plug is made of a silicone material, the set is recessed with a groove, and the inner side of the groove is tapered toward the bottom of the groove. . The cell storage container according to claim 4, wherein the plug is provided with a convex portion between the set portion and the pin portion, and the convex portion abuts against one side of the open end In the periphery, the cover system has a plurality of slots, the bottom of the slot of the set of slots is through the through hole, and the bottom of the groove of the set of slots is pressed against the protrusion. The cell storage container according to the first aspect of the invention, wherein the cover body is convexly provided with a protruding post, the protruding post is provided with the through hole, and the outer peripheral side of the protruding post is surrounded and protruded. There is at least one flange, and the inner peripheral side of the plug cover is provided with a card slot corresponding to the flange, and the flange is correspondingly engaged with the card slot for the plug cover to be engaged with the convex cover column. The cell storage container according to the sixth aspect of the invention, wherein the outer peripheral side of the plug cover is provided with a dial block, and the bottom side of the plug cover is provided with a slope toward the inner peripheral side. The slope is configured to allow the plug cover to be quickly assembled to the post and to engage the flange in the slot. The cell storage container according to claim 1, wherein the closed end of the storage tube is circumferentially surrounded by a standing portion. The cell storage container according to claim 1, wherein the accommodating space is formed in an inverted arc shape adjacent to the closed end. The cell storage container according to claim 1, wherein the outer peripheral side of the cover is surrounded by a plurality of protruding ribs.