TW201807190A - Fermenting system and procedure of fermenting - Google Patents

Abstract

A procedure of fermenting includes the following steps. Sterilizing of a first fermenter; loading a medium into the first fermenter wherein the medium passes through a sterilizing device from a medium source before being loaded into the first fermenter; loading a microbial culture into the first fermenter from a culture source; fermenting to form a first product; discharging the first product.

Description

Fermentation manufacturing system and fermentation process

The present invention relates to a fermentation manufacturing system and a fermentation process.

In the conventional fermentation process, the fermentation tank is first sterilized, and then the culture medium is put into the culture medium, and then the tank is heated and sterilized, and then cooled and then put into the strain for fermentation. After the fermentation is completed and discharged, the fermentation tank is cleaned. Once again sterilized, the medium can be re-introduced for the next fermentation process.

However, the above process has many disadvantages: First, due to the large volume of the fermentation tank, the time for heating and sterilization is often required to be at least two hours, and the sterilization temperature must be higher than 150 ° C. However, it is necessary to cool a whole barrel of the medium until it can be carried out. Fermentation takes another 2 to 3 hours. The whole fermentation process is extremely time consuming, energy consuming, and there is a possibility that some of the medium can be coked. Furthermore, after each fermentation, it is necessary to clean and resterilize before re-injecting new medium. The fermentation process is also lengthened and cannot be continuously manufactured.

The main object of the present invention is to provide a fermentation manufacturing system and a fermentation process, which can simplify the process and improve the efficiency.

In order to achieve the above object, the present invention provides a fermentation manufacturing system comprising a first fermentation tank, a sterilization mechanism, a medium supply source and a strain supply source.

The first fermentation tank has a confined space and has a first medium inlet, a first species inlet and a first discharge port; the sterilization mechanism is connected to the first medium inlet of the first fermentation tank by a pipeline a medium; the medium supply source is connected to the sterilization mechanism by a pipeline to supply a culture medium to the sterilization mechanism; the strain supply source is connected to the first strain inlet of the first fermentation tank by the pipeline to a fermenting tank supplies a strain; wherein the medium supply source supplies the medium to the sterilizing mechanism, and the medium is sterilized by the sterilizing mechanism, and after cooling, enters the first fermenting tank through the first medium inlet. The strain supply source is introduced into the first fermentation tank via the first strain inlet, and the medium is fermented in the first fermentation tank to form a first product, the first product. The first fermentation tank is exited via the first discharge port.

In order to achieve the above object, the present invention further provides a fermentation process comprising the steps of: sterilizing in a tank: sterilizing the inside of a first fermentation tank; and inputting the medium: inputting a medium into the closed space of the first fermentation tank, wherein The culture medium is supplied from a medium supply source to a sterilization mechanism for sterilization, and then is introduced into the first fermentation tank via a pipeline; the input strain: a strain supply source inputs a strain to the sealed space of the first fermentation tank; Fermentation: the medium is fermented with the strain in a confined space of the first fermentation tank to form a first product; and the first product is discharged from the first fermentation tank.

Thereby, the fermentation manufacturing system and the fermentation process provided by the invention can simplify the process, eliminate the cumbersome steps of cleaning and sterilizing the fermentation tank, and shorten the time required for manufacturing.

The following is a description of the possible embodiments of the present invention, and is not intended to limit the scope of the invention as claimed.

Referring to FIG. 1, the present invention provides a fermentation manufacturing system comprising a first fermentation tank 10, a sterilization mechanism 20, a medium supply source 30, and a strain supply source 40.

The first fermentation tank 10 has a closed space and has a first medium inlet 11, a first strain inlet 12 and a first discharge port 13; the sterilization mechanism 20 is connected to the first fermentation tank by a pipeline a first medium inlet 11 of 10 is connected; the medium supply source 30 is connected to the sterilization mechanism 20 by a pipeline to supply a culture medium to the sterilization mechanism 20; the strain supply source 40 is connected to the first fermentation tank by a pipeline a first strain inlet 12 of 10 is connected to supply a strain to the first fermenting tank 10; wherein the tubing between the sterilizing mechanism 20 and the first medium inlet 11 , the medium supply source 30 and the sterilizing mechanism The pipeline between the 20, the supply source 40 of the strain and the inlet 12 of the first species are respectively provided with a valve body to control the connection or not.

Thereby, the medium supply source 30 supplies the culture medium to the sterilizing mechanism 20, and the medium is sterilized by the sterilizing mechanism 20 (preferably, heated at a temperature of 110 ° C to 150 ° C for about 20 minutes). Cooling in the pipeline leading to the first medium inlet 11 (also in the sterilization mechanism, that is, cooling), entering the first fermentation tank 10 via the first medium inlet 11 , the strain supply source 40 (preferably comprising a peristaltic motor), the strain is introduced into the first fermentation tank 10 via the first strain inlet 12, and the medium is fermented with the strain in the first fermentation tank 10 to form a first The product, the first product exits the first fermentation tank 10 via the first discharge port 13.

In this embodiment, the sterilization mechanism includes a high temperature sterilization portion and a low temperature cooling portion, thereby completing the sterilization and cooling steps in the sterilization mechanism.

In particular, as shown in FIG. 1A, the strain supply source 40 can also be connected to the pipeline between the first fermentation tank 10 and the sterilizing mechanism 20, and the strain can enter the first fermentation tank in the cooled medium. After 10, the strain is introduced into the first fermentation tank 10 by a shared line.

With the above system, as shown in FIG. 2, the present invention further provides a fermentation process comprising the following steps:

In-tank sterilization S11: Sterilization of the inside of a first fermentation tank.

The medium S12 is input: a medium is introduced into the sealed space of the first fermentation tank, wherein the medium is supplied to a sterilization mechanism for sterilization by a medium supply source, and then is introduced into the first fermentation tank via a pipeline.

The input strain S13: a strain supply source inputs a strain to the sealed space of the first fermentation tank.

Fermentation S14: The medium is fermented with the strain in a confined space of the first fermentation tank to form a first product 101.

Discharge S15: The first product 101 is discharged from the first fermentation tank. After the discharge, if the next fermentation is to be continued, the washing and the sterilization tank are not required to be sterilized, and the step of returning to the medium can be directly performed.

Of course, after the step of discharging S15, the steps of sequentially inputting the medium S12, introducing the strain S13, fermenting S14, and discharging S15 may be continued to form continuous fermentation production.

In the second embodiment of the present invention, as shown in FIG. 3, the fermentation manufacturing system further includes a second fermentation tank 50 having a closed space and having a second medium inlet 51. a second strain inlet 52 and a second outlet 53, the second medium inlet 51 and the sterilizing mechanism 20 are connected via a pipeline to enable the medium to enter the second fermentation tank 50, the second strain inlet 52 Connecting with the first discharge port 13 to enable the first product to enter the second fermentation tank 50, and the medium and the first product are fermented in the second fermentation tank 50 to form a second product. The second product exits the second fermentation tank 50 by the second discharge port 53, wherein the volume of the second fermentation tank 50 is larger than the volume of the first fermentation tank 10; and even more includes a third fermentation tank 60, The third fermentation tank 60 has a closed space and has a third medium inlet 61, a third strain inlet 62 and a third discharge port 63. The third medium inlet 61 is connected to the sterilization mechanism 20 via a pipeline. The medium is allowed to enter the third fermentation tank 60, the third species inlet 62 The second discharge port 53 is connected to enable the second product to enter the third fermentation tank 60, and the medium and the second product are fermented in the third fermentation tank 60 to form a third product, wherein the second product The volume of the three fermenters 60 is greater than the volume of the second fermentor 50.

Referring to FIG. 4 again, the present invention and thereby provide a fermentation process, which includes the following steps in addition to the steps already mentioned in the first embodiment:

In-tank sterilization S21: Sterilization of the inside of a second fermentation tank.

The medium S22 is introduced: a medium is introduced into the sealed space of the second fermentation tank, wherein the medium is supplied to the sterilization mechanism for sterilization by the medium supply source, and then is introduced into the second fermentation tank via a pipeline.

The first product S23 is charged: the first product 101 is introduced into the sealed space of the second fermentation tank.

Fermentation S24: The medium is fermented with the first product 101 in a confined space of the second fermentation tank to form a second product 501.

Discharge S25: The second product 501 is discharged from the second fermentation tank.

In-tank sterilization S31: Sterilize the inside of a third fermentation tank.

The medium S32 is introduced: a medium is introduced into the sealed space of the third fermentation tank, wherein the medium is supplied to the sterilization mechanism for sterilization by the medium supply source, and then is introduced into the third fermentation tank via a pipeline.

The second product S33 is introduced: the second product 501 is introduced into the sealed space of the third fermentation tank.

Fermentation S34: The medium and the second product 501 are fermented in a closed space of the third fermentation tank to form a third product 601.

Discharge S35: The third product 601 is discharged from the third fermentation tank.

In particular, after the fermentation tank has completed the discharge step, if the next fermentation is to be continued, the step of returning to the medium can be directly returned, and no cleaning or tank sterilization is required.

Thereby, the fermentation product of the previous fermentation tank can be used as the fermentation strain of the next fermentation tank to save the fermentation time and achieve the effect of continuous fermentation expansion, and even have the fourth fermentation tank and the fifth fermentation. More fermenters such as tanks are used to extend the process of continuous fermentation expansion.

In summary, the present invention sterilizes the culture medium by a sterilization mechanism independent of the fermentation tank, and the culture medium is dynamically passed through the sterilization mechanism and the pipeline, and the heating efficiency is excellent, and the sterilization can be quickly cooled after the sterilization is completed. The cooling temperature is reached before the fermentation tank, which saves the process time, and the feed fermentation can be continued after the discharge is completed. It does not need to be cleaned and sterilized every time, which greatly improves the production efficiency. If more than one fermentation tank is connected in series, it can further Improve manufacturing efficiency, and avoid the problem of coking of the medium, which is very effective.

10‧‧‧First fermentation tank
101‧‧‧ first product
11‧‧‧First medium inlet
12‧‧‧First species entry
13‧‧‧First discharge opening
20‧‧‧Sterilization agency
30‧‧‧Supply source
40‧‧‧Supply source
50‧‧‧Second fermentation tank
501‧‧‧ second product
51‧‧‧Second medium inlet
52‧‧‧Second strain entry
53‧‧‧Second outlet
60‧‧‧ Third fermentation tank
601‧‧‧ third product
61‧‧‧ third medium inlet
62‧‧‧ third strain entry
63‧‧‧ Third discharge opening
S11‧‧‧ In-slot sterilization
S12‧‧‧ input medium
S13‧‧‧Invested strains
S14‧‧‧ Fermentation
S15‧‧‧Sewage
S21‧‧‧ In-slot sterilization
S22‧‧‧ input medium
S23‧‧‧Invest in the first product
S24‧‧‧ Fermentation
S25‧‧‧Sewage
S31‧‧‧ In-slot sterilization
S32‧‧‧ input medium
S33‧‧‧Invest in the second product
S34‧‧‧ Fermentation
S35‧‧‧Sewage

BRIEF DESCRIPTION OF THE DRAWINGS Figure 1 is a schematic view showing a first embodiment of the present invention. 1A is a schematic view of another embodiment of the present invention. Figure 2 is a flow chart of the first embodiment of the present invention. Figure 3 is a schematic view of a second embodiment of the present invention. Figure 4 is a flow chart of a second embodiment of the present invention.

Claims (16)

A fermentation manufacturing system comprising: a first fermentation tank having a confined space and having a first medium inlet, a first species inlet and a first outlet; a sterilization mechanism, by the pipeline a first medium inlet connection of the first fermentation tank; a medium supply source connected to the sterilization mechanism by a pipeline to supply a culture medium to the sterilization mechanism; a strain supply source through the pipeline and the first fermentation tank The first strain is connected to the first fermenter to supply a strain; wherein the medium supply source supplies the medium to the sterilizing mechanism, and the medium is sterilized by the sterilizing mechanism, and then cooled The first medium inlet enters the first fermentation tank, and the strain supply source inputs the strain into the first fermentation tank via the first strain inlet, and the medium and the strain are fermented in the first fermentation tank A first product is then formed, the first product exiting the first fermentation tank via the first discharge port. The fermentation manufacturing system of claim 1, wherein the pipeline between the sterilization mechanism and the first medium inlet further has a valve body, and the pipeline between the medium supply source and the sterilization mechanism also has a valve body. The sterilization mechanism comprises a high temperature sterilization portion and a low temperature cooling portion. The fermentation manufacturing system according to claim 1, wherein the culture medium stays in the sterilization mechanism for 20 minutes, and the sterilization temperature is 110 ° C to 150 ° C. The fermentation manufacturing system of claim 1, further comprising a second fermentation tank having a closed space and having a second medium inlet, a second strain inlet and a second outlet The second medium inlet is connected to the sterilizing mechanism via a pipeline to enable the medium to enter the second fermentation tank, and the second strain inlet is connected to the first discharge port to enable the first product to enter the second fermentation In the tank, the medium and the first product are fermented in the second fermentation tank to form a second product, and the second product leaves the second fermentation tank through the second discharge port, wherein the second fermentation The volume of the tank is greater than the volume of the first fermentation tank. The fermentation manufacturing system of claim 5, further comprising a third fermentation tank having a closed space and having a third medium inlet, a third strain inlet and a third outlet The third medium inlet is connected to the sterilizing mechanism via a pipeline to enable the medium to enter the third fermentation tank, and the third strain inlet is connected to the second discharge port to enable the second product to enter the third fermentation. In the tank, the medium and the second product are fermented in the third fermentation tank to form a third product, wherein the volume of the third fermentation tank is greater than the volume of the second fermentation tank. The fermentation manufacturing system of claim 1, wherein the strain supply source comprises a peristaltic motor. A fermentation manufacturing system comprising: a first fermentation tank having a confined space; a sterilization mechanism connected to the first fermentation tank by a pipeline; a medium supply source connected to the sterilization mechanism by a pipeline to The sterilizing mechanism supplies a culture medium; a strain supply source is connected to the pipeline connected to the sterilizing mechanism and the first fermentation tank by a pipeline to supply a strain to the first fermentation tank; wherein The medium supply source supplies the culture medium to the sterilizing mechanism, and the medium is sterilized by the sterilizing mechanism, and after cooling, enters the first fermentation tank, and the strain supply source puts the strain into the first fermentation tank. The medium is fermented with the strain in the first fermentation tank to form a first product, and the first product leaves the first fermentation tank. The fermentation manufacturing system of claim 7, wherein the pipeline between the sterilization mechanism and the first fermentation tank further has a valve body, and the pipeline between the medium supply source and the sterilization mechanism also has a valve body. The sterilization mechanism comprises a high temperature sterilization portion and a low temperature cooling portion. The fermentation manufacturing system according to claim 7, wherein the culture medium stays in the sterilization mechanism for 20 minutes, and the sterilization temperature is 110 ° C to 150 ° C. The fermentation manufacturing system of claim 7, further comprising a second fermentation tank having a closed space, the second fermentation tank being connected to the sterilization mechanism via a pipeline to enable the medium to enter the second a fermentation tank, the second fermentation tank is connected to the first fermentation tank to enable the first product to enter the second fermentation tank, and the medium and the first product are fermented in the second fermentation tank to form a second a product, wherein the volume of the second fermentation tank is greater than the volume of the first fermentation tank. The fermentation manufacturing system of claim 10, further comprising a third fermentation tank having a closed space, the third fermentation tank being connected to the sterilization mechanism via a pipeline to enable the medium to enter the third a fermentation tank, the third fermentation tank is connected to the second fermentation tank to enable the second product to enter the third fermentation tank, and the medium and the second product are fermented in the third fermentation tank to form a third a product, wherein the volume of the third fermentation tank is greater than the volume of the second fermentation tank. The fermentation manufacturing system of claim 7, wherein the strain supply source comprises a peristaltic motor. A fermentation process comprising the steps of: sterilizing in a tank: sterilizing a inside of a first fermentation tank; and inputting a medium: a medium is input into a closed space of the first fermentation tank, wherein the medium is supplied from a medium supply source a sterilizing mechanism is sterilized and then put into the first fermentation tank via a pipeline; the input strain: a strain supply source inputs a strain into the sealed space of the first fermentation tank; fermentation: the medium and the strain are Fermentation is carried out in the closed space of the first fermentation tank to form a first product; discharge: the first product is discharged from the first fermentation tank. According to the fermentation process described in claim 13, after the "discharging" step, the steps of "inputting the medium", "injecting the strain", "fermenting", and "discharging" are sequentially continued. The fermentation process as claimed in claim 13 further comprising the steps of: sterilizing in the tank: sterilizing the inside of a second fermenting tank; and feeding the medium: introducing a medium into the closed space of the second fermenting tank, wherein the medium is The medium is supplied to the sterilization mechanism for sterilization, and then is introduced into the second fermentation tank via a pipeline; the first product is introduced: the first product is introduced into the sealed space of the second fermentation tank; fermentation: the medium and the medium The first product is fermented in a closed space of the second fermentation tank to form a second product; and the second product is discharged from the second fermentation tank. The fermentation process as claimed in claim 13 further comprising the steps of: sterilizing in the tank: sterilizing the inside of a third fermentation tank; and inputting the medium: introducing a medium into the closed space of the third fermentation tank, wherein the medium is The medium supply source is supplied to the sterilization mechanism for sterilization, and then is introduced into the third fermentation tank via a pipeline; the second product is introduced: the second product is introduced into the sealed space of the third fermentation tank; fermentation: the medium and the medium The second product is fermented in a closed space of the third fermentation tank to form a third product; and the third product is discharged from the third fermentation tank.