TW201726157A - An allergy-inhibiting sea grape extract, its preparation method and thereof - Google Patents

Abstract

Present invention relates to an allergy-inhibiting sea grape extract, its preparation method and thereof. The preparation method includes following steps: the sea grape is blended in distilled water in the weight ratio of 1:1~20, mix thoroughly, stir at 20~100 DEG C for 30~120 minutes. The fluid obtained after the first filtration is the sea grape extract. The sea grape extract can be subjected to the second filtration by using EW, PW and DK membranes and freeze-dried to give a refined sea grape extract. The invention exhibits superior allergy-inhibiting effect; moreover, the prepared extract does not cause allergy and can be used in cosmetics and food.

Description

Sea grape extract with allergy inhibition, preparation method and application thereof

The invention relates to a sea grape extract, in particular to a sea grape extract which can inhibit allergy and does not cause allergy, and a preparation method and application thereof.

Allergies are a phenomenon in which the body's immune system is abnormal and overreacts to substances in the environment (also known as allergens). Allergens that enter the body bind to IgE antibodies. At this time, it will stimulate mast cells to release inflammatory substances such as histamine, which will cause inflammatory reactions in body tissues. Allergic symptoms will also be different due to the release of histamine body parts, including runny nose, tears, itching, shortness of breath and Symptoms such as dry skin may cause shock and endanger life. When allergic people are allergic to it, they usually use antihistamines and other drugs to relieve allergic symptoms. Allergic drugs generally have certain side effects. Therefore, natural plants or extracts from plants are used to suppress allergic activity. It is also a topic of public concern.

The sea grape belongs to the green algae. The sea grape is not only rich in seaweed polysaccharide, but also has zero fat prevention, low calorie, and fresh and crisp taste. Like caviar, it is the best natural thing for Japanese and many experts. Health foods are also high-quality ingredients in Japanese restaurants. And its large amount of trace elements have anti-aging effects, therefore, the sea grape extract has been included in the legal use of cosmetic raw materials by the World Cosmetics Association.

In the past, sea grape extraction technology was mostly extracted with alcohol or other organic solvents. Taiwan patent I484966 is a method for extracting sea grape extract, which is based on alcohol. The crude extract is separated into n-hexane and ethyl acetate, and the effective substance has the effect of inhibiting cancer. Korean Patent KR101252149 uses water and C1-C4 alcohol to prepare sea grape extract, which has anti-oxidation and anti-oxidation. Inflammation, moisturizing and other effects. The prior art sea grape extract has no anti-allergic effect, and extracting sea grapes with an organic solvent may cause harm to the human body even if only a small amount of organic solvent remains.

The prior art sea grape extraction technology mostly uses organic solvents, and a small amount of organic solvent residues may cause harm to human health. Because of the different polarities of various solvents, the extraction ability is also different. If no organic solvent is used, how to Extracting a sea grape extract having an allergy-preventing effect is an important subject to be solved by the present invention.

In view of the deficiencies and shortcomings of the prior art, the inventors of the present invention wish to provide a natural and safe sea grape extract, which is extracted without extracting with an organic solvent, extracting sea grapes with water at a specific temperature and time, and further filtering the film to obtain sea grapes. An allergic active substance.

The object of the present invention is a method for extracting sea grape extract, comprising the following steps: Step 1: Beating sea grapes with pure water, wherein the weight ratio of the sea grapes to pure water is 1 part of sea grapes: pure water 1~20 parts, blended evenly with sea grape pulp; Step 2: sea grape pulp continues to stir, stirring temperature is 20~100 °C, stirring time is 30~120 minutes; Step 3: first filtration, after the first time The liquid obtained by filtration obtains a sea grape extract.

For the purpose of the foregoing invention, in the step 1, the sea grape may be fresh sea grapes, Dry sea grapes, or sea grape powder.

In order to achieve the above object, in the step 2, the stirring temperature is 60 to 90 ° C; preferably, the stirring temperature is 85 ° C; the stirring temperature is 60 to 105 minutes; preferably, the stirring The temperature is 90 minutes.

For the purpose of the foregoing invention, in the step 3, the conditions of the first filtration are 5 micrometers and 1 micrometer.

For the purpose of the foregoing invention, the second filtration may be further performed after the first filtration, and the second filtration is membrane filtration; the first membrane of the membrane filtration treatment is GE EW membrane, and the second membrane is GE's PW film and the third film are GE's DK film.

Another object of the present invention is an allergy-free sea grape extract obtained by the above extraction method of sea grape extract.

Another object of the invention is a composition comprising an effective amount of an allergenic sea grape extract and a cosmetic, food or pharmaceutically acceptable excipient, wherein the composition is a cosmetic composition, a food product A composition or pharmaceutical composition.

For the purpose of the foregoing invention, the effective amount of the sea grape extract is 1 mg/ml or more; preferably, the effective amount of the sea grape extract is 4 mg/ml.

For the purpose of the foregoing invention, the cosmetic composition is present in a form selected from the group consisting of creams, multiple emulsions, anhydrous compositions, aqueous dispersions, oils, emulsions, balms, foams, lotions, coagulations. Glue, latex, aqueous ethanol solution, hydroglycolic solution, hydrogel, coating agent, serum, soap, shampoo, conditioner, serum, ointment, mousse, hair cream, powder, Strips, pens, sprays, aerosols, capsules, gelatin capsules, soft capsules, hard capsules, lozenges, sugar-coated tablets, granules, chewing gums, solutions, suspensions, lotions, syrups, Sugar film, jelly and gelatin.

For the purpose of the foregoing invention, the cosmetic composition may be incorporated into a product selected from the group consisting of an eye cream, a foundation, a makeup remover, a makeup remover, an eye shadow, a lipstick, a lip gloss, a lip balm, and a honey powder.

In order to achieve the above object, the shampoo component is 10~50% of surfactant, 0.3~2% of emollient, 0.01~0.1% of sea grape extract, 0~2% thickener, and 0.1~ 1%, preservative 0.3~1%, the rest is water.

Figure 1 is an allergic induction reaction, calcium ionophore A23187 stimulates cell degranulation to release β- Hexosaminidase, the effect of sea grape extract on the release of β-hexosaminidase.

Figure 2 shows the effect of sea grape extract on allergy inhibition.

Figure 3 shows the effect of sea grape extract on allergy inhibition.

All of the technical and scientific terms described in this specification, unless otherwise defined, are intended to be common to those of ordinary skill in the art.

The present invention is exemplified by the following examples, but the present invention is not limited by the following examples.

Example 1

1. Preparation method of sea grape extract (I)

Take 80g of sea raisin powder and 1600g of pure water. The sea grape used is Caulerpa lentillifera , which is evenly mixed (temperature is about 85 ° C, time is about 90 minutes), and then filtered with 5 μm and 1 μm sieve. The liquid sea grape extract (I) is obtained; and the membrane filtration is performed. The first film is GE EW film, the microfiltration removes large impurities, and the second film is GE PW film, which is ultrafiltration (Ultrafiltration). The fine impurities were removed, and the third film was a GE DK film, and the nanofiltration was used to remove the salt, and the film was filtered and freeze-dried to obtain a sea grape extract (I). The above-mentioned long-stem grape fern algae can also be replaced by other varieties of sea grapes such as Caulerpa taxifolia .

Second, sea grape extract (I) does not cause allergies

Cells were supplemented with sea grape extract (I) and sea grape extract (I) without the use of stimulant (A23187(-)) to observe the release of the allergic medium β-hexosaminidase (Fig. 1), sea grape extract (I) Compared with sea grape extract (I) alone and control group, sea grape extract (I) and sea grape extract (I) alone did not increase β-hexosaminidase release, indicating sea grape extract (I ) and sea grape extract (I) itself does not trigger allergies.

Third, allergy induction experiment

The calcium ionophore A23187 stimulated the cells to degranulate and release the allergic medium β-hexosaminidase (β-hexosaminidase). The β-hexosaminidase concentration was detected by enzyme-linked immunosorbent assay (ELISA). As a result of Ctr, it was revealed that A23187 stimulation induced β-hexosaminidase release.

Fourth, sea grape extract (I) inhibits allergies

According to the results in Figure 2, sea grape extract (I) inhibited the release of β-hexosaminidase induced by A23187 stimulation (A23187(+) group), and the release of β-hexosaminidase by sea grape extract (I) 2 mg/ml was reduced by 8 %, sea grape extract (I) 4mg/ml reduced β-hexosaminidase release by 25%; sea grape extract (I) inhibited β-hexosaminidase release compared with sea Portuguese The extract was more obvious. The extract of sea grape extract (I) 1 mg/ml inhibited the release of β-hexosaminidase by 45%, and the extract of sea grape extract (I) 4 mg/ml inhibited the release of β-hexosaminidase by 82% without affecting Cell viability (Figure 1).

5. Cosmetic application of sea grape extract (I)

Quantitative pure water is heated to 50~60 °C, the surfactant is added sequentially, and the mixture is stirred and evenly mixed. Add the emollient in sequence and mix well. Stop heating, wait until the temperature drops to 30 ° C, add sea grape extract (I), sweet orange essential oil, sodium anisate and mix well to obtain mousse shampoo containing sea grape extract (I). The mousse shampoo formula is shown in Table 1, for example.

Sixth, sea grape extract (I) human body experiment made into cosmetics

Entrusted the Food and Drug Administration (FDA) accredited laboratory AMA Laboratories Inc. to carry out mousse shampoo (preparation ratio, such as Table 1) containing sea grape extract (I) to produce skin irritation and In the human test of sensitivity test, the number of subjects in this human experiment was 52, and the number of subjects completing the study was 50. The age range of the subjects was 20-68 years old, and the gender and ethnicity are shown in Table 2.

In the human body, the mousse shampoo shown in Table 1 was diluted 10 times with water, and 0.2 ml of diluted mousse shampoo was applied to the semi-closed hypoallergenic patch, which was applied to the subscapular area of the subject. The back of the skin, the left or right side of the midline, and indicate that the subject is not wet or direct sunlight. After 24 hours, the subject tears off the patch at home and repeats the procedure until a series of Nine exposures were completed, and were performed every Monday, Wednesday, and Friday for three consecutive weeks. If adverse reactions occurred, the areas of erythema and edema were measured. The results are shown in Table 3. 50 subjects completed the study, There is no allergic reaction in the mousse shampoo of sea grape extract (I), indicating that the mousse shampoo containing sea grape extract (I) has no skin irritation and does not cause allergies.

Example 2

Take 1.5 kg of fresh sea grapes and 1.5 kg of pure water. The sea grapes used are Caulerpa lentillifera , mixed evenly (temperature about 85 ° C / time about 30 minutes), then 5 μm and 1 μm filter Filtration, the liquid is the sea grape extract (II); and then membrane filtration, the first film is GE EW film, microfiltration (Microfiltration) to remove large impurities, the second film is GE PW film, is ultrafiltration (Ultrafiltration) removes fine impurities, the third film is GE DK film, nanofiltration removes salt, and the film is filtered and freeze-dried to obtain sea grape extract (II). The above-mentioned long-stem grape fern algae can also be replaced by other varieties of sea grapes such as Caulerpa taxifolia .

According to the results in Fig. 3, sea grape extract (II) can inhibit the release of β-hexosaminidase induced by A23187 (A23187(+) group), and the extract of sea grape extract (II) can inhibit β at a concentration of 1 mg/ml. - hexosaminidase is released 40%, concentration 2mg/ml can inhibit the release of β-hexosaminidase by 60%, and concentration of 4mg/ml can inhibit the release of β-hexosaminidase by 80%.

Example 3

Take 1.5 kg of fresh sea grapes and 1.5 kg of pure water. The sea grapes used are Caulerpa lentillifera , which is evenly mixed (temperature is about 25 ° C / time about 60 minutes), then 5 μm and 1 μm sieve. Filtration, the liquid is obtained from the sea grape extract (III); the membrane is filtered, the first film is GE EW film, the microfiltration (Microfiltration) removes large impurities, and the second film is GE PW film, which is ultrafiltration. (Ultrafiltration) removes fine impurities, the third film is GE DK film, nanofiltration removes salt, and the film is filtered and freeze-dried to obtain sea grape extract (III). The above-mentioned long-stem grape fern algae can also be replaced by other varieties of sea grapes such as Caulerpa taxifolia .

According to the results in Fig. 3, sea grape extract (III) can inhibit the release of β-hexosaminidase induced by A23187 (A23187(+) group); the extract of sea grape extract (III) can inhibit β- at a concentration of 1 mg/ml. Hexosaminidase released 33%, concentration of 2mg/ml inhibited the release of β-hexosaminidase by 56%, and concentration of 4mg/ml inhibited the release of β-hexosaminidase by 75%.

The detailed description of the preferred embodiments of the present invention is not intended to limit the scope of the present invention, and the equivalent implementations or modifications of the present invention should be included in the present invention. In the scope of patents.

The above-mentioned multiple functions are the statutory patent requirements that fully meet the novelty and progressiveness. If you apply in accordance with the law, you are requested to approve the application for the invention patent to encourage the invention.

Claims (16)

The method for extracting sea grape extract comprises the following steps: Step 1: Beating sea grape and pure water, wherein the weight ratio of the sea grape to the pure water is 1 part of sea grape: 1-20 parts of pure water, and the mixture is evenly mixed. Dehai grape pulp; Step 2: sea grape pulp continues to stir, stirring temperature is 20~100 °C, stirring time is 30~120 minutes; Step 3: performing the first filtration, the liquid obtained by the first filtration is the sea Grape extract. For example, in the extraction method of the sea grape extract of claim 1, in the step 1, the sea grape may be fresh sea grapes, dry sea grapes, or sea grape powder. For example, in the extraction method of the sea grape extract of claim 1, the stirring temperature is 60 to 90 °C. For example, in the extraction method of the sea grape extract of the first aspect of the patent application, in the step 2, preferably, the stirring temperature is 85 °C. For example, in the extraction method of the first grape extract of the patent scope, in the step 2, the stirring time is 60 to 105 minutes. For example, in the extraction method of the sea grape extract of the first aspect of the patent application, in the step 2, preferably, the stirring time is 90 minutes. For example, in the extraction method of the sea grape extract of claim 1, the first filtration condition is 5 micrometers and 1 micrometer. For example, in the extraction method of the sea grape extract of claim 1, in the step 3, the second filtration may be further performed after the first filtration; the second filtration is membrane filtration. For example, in the extraction method of the sea grape extract of claim 8th, the first film of the film filtration treatment is GE EW film, the second film is GE PW film, and the third film is GE company. DK film. An allergic sea grape extract obtained by the extraction method of sea grape extract of any one of claims 1 to 9. A composition comprising an effective amount of an allergy-suppressing sea grape extract and a cosmetic, food or pharmaceutically acceptable excipient as claimed in claim 10, wherein the composition is a cosmetic composition, A food composition or pharmaceutical composition. The composition of the marine grape extract of claim 11 is effective in an amount of 1 mg/ml or more. The sea grape extract effective amount is 4 mg/ml as in the composition of claim 11 of the patent application. The composition of claim 11, wherein the cosmetic composition is in a form selected from the group consisting of creams, multiple emulsions, anhydrous compositions, aqueous dispersions, oils, emulsions, balms, foams , lotion, gel, latex, aqueous ethanol solution, hydroglycolic solution, hydrogel, coating agent, serum, soap, shampoo, conditioner, serum, ointment, mousse, Hair cream, powder, strip, pen, spray, aerosol, capsule, gelatin capsule, soft capsule, hard capsule, lozenge, sugar coating, granule, chewing gum, solution, suspension, emulsion, syrup, polysaccharide film, glue Frozen and gelatin. The composition of claim 11, wherein the cosmetic composition can be incorporated into a product selected from the group consisting of an eye cream, a foundation, a cleansing lotion, a cleansing lotion, an eye shadow, a lipstick, a lip gloss, a lip balm, and a honey powder. . According to the composition of claim 14 of the patent scope, the shampoo component is 10~50% of surfactant, 0.3~2% of emollient, 0.01~0.1% of sea grape extract, 0~2% of thickener. The fragrance is 0.1~1%, the preservative is 0.3~1%, and the rest is water.