TW201703648A - Thermal reaction spice with seafood flavor prepared from dried winter mushrooms for adding seafood flavor in pure vegetarian or meat dishes - Google Patents
Thermal reaction spice with seafood flavor prepared from dried winter mushrooms for adding seafood flavor in pure vegetarian or meat dishes Download PDFInfo
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本發明為一種以乾燥金針菇為基質製作具海產(魷魚)風味之素海產香料,並包含其製備方法,尤指一以經乾燥後,再儲藏一段時間之金針菇作為熱反應香料之基本材料,將其加工經加熱反應後製成具海產風味之香料,可應用於純素食或葷食食品料理中,為食品增添海產香氣。 The invention relates to a method for preparing a sea-based (sea bass) flavored sea-based spice by using a dried Flammulina velutipes as a substrate, and a preparation method thereof, in particular, a mushroom which is dried and then stored for a period of time as a basic material of a heat-reactive fragrance. The processed product is heated to produce a spice with a seafood flavor, which can be used in pure vegetarian or foraging foods to add aroma of seafood to the food.
近年素食養生風氣漸長,不論是宗教力量或健康因素,素食人口正逐漸增加。現在素食食品加工不再只單純限於植物原味,亦有素肉、素海產等具有肉香味或海鮮風味之素食加工食品,以增加素食者在素食選擇上地多樣化,同時兼具美味與健康,使得大眾對於素食的接受度增高。為增加素食加工食品具有肉類或海產類之香氣,以往以人工化學合成香料作為加工食品之調味方法,但已不符合現代人所追求的健康自然之要求。因此,食品加工業與食科研究開始致力於以天然植物製成具有葷食香氣之香料。 In recent years, vegetarian diet has become more and more popular, and the vegetarian population is gradually increasing, regardless of religious strength or health factors. Nowadays, vegetarian food processing is no longer limited to the original flavor of plants. It also has vegetarian or processed foods such as vegetarian meat and vegetarian seafood, which can increase the variety of vegetarians in vegetarian choices, and at the same time have both delicious and healthy. The public's acceptance of vegetarian diet has increased. In order to increase the aroma of meat or seafood in vegetarian processed foods, artificial chemical synthetic flavors have been used as a seasoning method for processed foods, but they are not in line with the health and natural requirements pursued by modern people. Therefore, the food processing industry and food research have begun to work on natural plants to make spices with aromas of foraging.
如專利申請號第96134427號,其發明係以香椿製作肉精風味之調味料的方法、製成品及其應用。乃係利用天然植物 香椿以乳酸菌發酵技術處理並加熱製成具有肉香味之調味料,可適用於純素食食品中。雖然如此,目前並無具有海產風味之素食香料被開發出,而目前之素海產香料乃係利用海產萃取液再添加胺基酸及糖,以加熱產生梅納反應所製成,嚴格而言,製程並非純素。 For example, Patent Application No. 96134427, the invention is a method, a finished product and an application thereof for making a seasoning of a meat flavor with a citron. Natural plants The camphor is treated with lactic acid bacteria fermentation technology and heated to make a seasoning with meat flavor, which can be applied to pure vegetarian food. Even so, there is no vegetarian flavor with a seafood flavor. At present, the vegetarian flavor is made by using a marine extract and adding an amino acid and sugar to heat the Mena reaction. Strictly speaking, The process is not vegan.
為突破純素食者對於具海產風味之素食加工食品的 要求,同時欲符合天然食品安全要求,本發明遂利用乾燥金針菇作為基質,利用加熱反應技術開發具海產風味之熱反應香料。 For the breakthrough of vegans for vegetarian foods with seafood flavors In order to meet the requirements of natural food safety at the same time, the present invention utilizes dried Flammulina velutipes as a substrate, and uses a heating reaction technique to develop a hot reaction flavor having a seafood flavor.
金針菇(Flammulina velutipes)為口蘑科、小火焰屬, 因子實體形狀似金針菜而得名,日本人稱之為enokitake mushroom,英文為winter mushroom,係全世界主要的食用菌之一。金針菇美味適口,口感佳,其營養豐富,高蛋白質、高纖維、低脂質,亦富含維生素、礦物質等,其中以離胺酸(Lysine)及精胺酸(Arginine)含量特別豐富,可促進兒童智力發展,素有增智菇之美名。 Flammulina velutipes is named for the genus Tricholoma, small flame, and the shape of the entity is like the ginseng. The Japanese call it enokitake mushroom. The English is winter mushroom, which is one of the world's major edible fungi. Flammulina velutipes is delicious and tasteful. It is rich in nutrients, high in protein, high in fiber and low in lipid. It is also rich in vitamins and minerals. It is especially rich in lysine and arginine. The development of children's intelligence is known as the wisdom of mushrooms.
專利申請號201310102804.8係揭露一種金針菇粉的 製備方法,以解決新鮮金針菇採收保鮮期短,難以儲藏運輸之問題。本發明著重於,在不同溫度下將新鮮金針菇進行乾燥並儲藏一段時間後,發現該乾燥金針菇會具有類似海產之香氣,以此將其加工製備作為素海產香料之應用。 Patent application number 201310102804.8 discloses a kind of mushroom powder The preparation method is to solve the problem that the fresh Flammulina velutipes has a short shelf life and is difficult to store and transport. The invention focuses on drying and storing fresh Flammulina velutipes at different temperatures for a period of time, and then discovering that the dried Flammulina velutipes has a similar aroma of marine products, thereby preparing and processing the same as a flavor of vegetarian seafood.
鑒於以上所述,本發明係利用天然植物-金針菇, 將其乾燥儲藏後加工製成具海產風味的純素香料。亦即本發明之一方面係關係於,一種素海產香料,其特徵在於係使用經過乾燥及儲藏之金針菇,與胺基酸、核苷酸、脂肪酸及糖類進行加熱反應而產生。 In view of the above, the present invention utilizes a natural plant, the enoki mushroom, It is dried and stored for processing to produce a vegan flavor with a seafood flavor. That is, one aspect of the present invention relates to a vegetarian aroma which is produced by heating and reacting a dried and stored Flammulina velutipes with an amino acid, a nucleotide, a fatty acid and a saccharide.
另一方面,本發明關於一種素海產香料的製造方 法,其包含:將金針菇經過於35-55℃下乾燥10-35小時及於35-55℃下儲藏5週預處理;將經過預處理之金針菇與精胺酸(Arginine)、丙胺酸(Alanine)、甘胺酸(Glycine)、牛磺酸(Taurine)及葡萄糖(Glucose)混合,並調整該混合物之pH值為pH 7.0-8.0;及將該混合物於100℃下加熱120分鐘,而製得具有海產風味的熱反應香料。 In another aspect, the present invention relates to a method for producing a vegetarian seafood. The method comprises the following steps: drying the Flammulina velutipes at 35-55 ° C for 10-35 hours and storing at 35-55 ° C for 5 weeks; pre-treating Flammulina velutipes with Arginine and Alanine , Glycine, Taurine, and Glucose are mixed, and the pH of the mixture is adjusted to pH 7.0-8.0; and the mixture is heated at 100 ° C for 120 minutes. A hot reaction flavor with a seafood flavor.
故本發明之目的在於利用天然植物金針菇,使其先 經由乾燥以及儲藏過程,接著與胺基酸和糖混合,再經熱反應加工後,而可調製為具海產風味之熱反應香料,其可應用於各類加工食品,同時亦符合天然安全健康之需求。 Therefore, the purpose of the present invention is to utilize the natural plant enoki mushroom to make it It can be prepared into a variety of processed foods through drying and storage processes, followed by mixing with amino acids and sugars, and then subjected to thermal reaction processing. It can be applied to all kinds of processed foods, and is also in line with natural safety and health. demand.
第1圖 素海產香料之製備方法 Figure 1 Preparation method of vegetarian seafood
本發明係關於一種以乾燥金針菇為基質所製成之素海產香料及其製備方法,且可用於加工食品之製作上。其中具海產風味的熱反應香料該製備方法主要為:將新鮮金針菇在35-55℃下乾燥10-35小時並於35-55℃下儲藏5週以取得乾燥金針菇,接著將其與胺基酸、核苷酸、脂肪酸及糖類混合進行加熱反應而成具有海產風味之熱反應香料。 The invention relates to a vegetarian seafood flavor prepared by using dried Flammulina velutipes as a substrate and a preparation method thereof, and can be used for the manufacture of processed foods. The preparation method of the hot reaction flavor with seafood flavor is mainly as follows: fresh Flammulina velutipes is dried at 35-55 ° C for 10-35 hours and stored at 35-55 ° C for 5 weeks to obtain dried Flammulina velutipes, and then with amino acid. A mixture of nucleotides, fatty acids and sugars is heated to form a hot reaction flavor with a seafood flavor.
以新鮮金針菇分別在35、40、45、50℃溫度下進行風乾並分別於該溫度儲藏5週的時間,而後將乾燥金針菇以喜好性官能品評試驗法進行風味評分,共以16位受訓過之品評者之7分法問卷做統計,對於不同溫度下乾燥儲藏之乾燥金針菇具有乾魷魚風味(Dried Squid Flavor)、飼料風味(Feed Flavor)及乾香菇風味(Dried Mushrooms Flavor)三個項目,依照喜好程度進行評分。每位品評員於無干擾狀況下,於室溫品評4種相同量的乾燥金針菇樣品,而樣品以亂數編碼進行盲測避免先入為主之觀念。 The fresh Flammulina velutipes were air-dried at 35, 40, 45, and 50 °C, respectively, and stored at this temperature for 5 weeks. Then, the dried Flammulina velutipes was tasted by the preferred functional evaluation method, and a total of 16 were trained. The evaluator's 7-point questionnaire is used for statistics. The dried Flammulina velutipes at different temperatures have three items: Dried Squid Flavor, Feed Flavor, and Dried Mushrooms Flavor. The degree is scored. Each of the product reviewers evaluated 4 samples of the same amount of dried Flammulina velutipes at room temperature without interference, and the samples were blindly coded to avoid preconceptions.
乾燥金針菇之喜好性官能品評試驗結果如表1所 示。由表1結果可得知,綜合16位品評者評分之結果,隨著乾燥與儲藏溫度之增高,乾燥金針菇所具有的乾魷魚風味就越加強烈,而飼料風味之呈味則反之;乾香菇風味則以45℃與50℃較為強烈。由此可知,新鮮金針菇本無明顯氣味,經由不同溫度之風乾並經過5周之儲藏時間,乾燥之金針菇會散發特殊海產味。 The test results of the favorite functional test of dried Flammulina velutipes are shown in Table 1. Show. It can be seen from the results of Table 1 that, as a result of the scores of 16 panelists, the dry squid has a stronger flavor of dried squid as the drying and storage temperature increases, while the taste of the feed flavor is reversed; The flavor is stronger at 45 ° C and 50 ° C. It can be seen that fresh Flammulina velutipes has no obvious odor, and it is dried by different temperatures and after 5 weeks of storage time, the dried Flammulina velutipes will emit special seafood flavor.
為進一步了解乾燥儲藏後之金針菇之海產味成分,本實施例選用分別以45℃與50℃乾燥與儲藏1至5周之乾燥金針菇,利用固相微萃取法(Solid Phase Microextraction,SPME)進行乾燥金針菇之香氣成分變化之分析。以SPME分析結果之中,分別鑑定出17種與18種揮發性成分包含醇類、酯類、醛類、酮類、酸類、氮氫化合物和雜環類等香氣化合物。其中,具有蝦、蟹海產香氣相關之化合物:雜環類化合物含2,5-dimethyl pyrazine或2,6-dimethyl pyrazine,醛類化合物如benzaldehyde,如表2、表3所示。同時可注意到的是,最具有海產香氣之50℃組別,隨著儲藏時間周數之增長,其香氣化合物之含量有逐漸增高的趨勢。由以上結果可知,經由乾燥及儲藏5週之過程,乾燥金針菇會逐漸產生海產風味香氣化合物。 In order to further understand the sea-flavored ingredients of Flammulina velutipes after dry storage, in this example, dried Flammulina velutipes which were dried and stored at 45 ° C and 50 ° C for 1 to 5 weeks, respectively, were dried by Solid Phase Microextraction (SPME). Analysis of the changes in the aroma components of Flammulina velutipes. Among the SPME analysis results, 17 and 18 volatile components were identified, including alcohols, esters, aldehydes, ketones, acids, nitrogen-hydrogen compounds, and heterocyclic compounds. Among them, there are compounds related to the aroma of shrimp and crab seaweed: the heterocyclic compound contains 2,5-dimethyl pyrazine or 2,6-dimethyl pyrazine, and the aldehyde compound such as benzaldehyde is shown in Table 2 and Table 3. At the same time, it can be noticed that the 50 °C group with the most aroma of seafood has a tendency to gradually increase the content of aroma compounds with the increase of the storage time. From the above results, it was found that the dried Flammulina velutipes gradually produced a marine flavor aroma compound after drying and storage for 5 weeks.
了解乾燥金針菇之香氣成分後,接下來進行熱反應香料之製備並進行香氣分析。主要製備過程如第1圖所示:以新鮮金針菇分別在35、40、45、50℃溫度下進行風乾並分別於該溫度儲藏5週之預處理,即得乾燥金針菇。將乾燥金針菇、精胺酸、丙胺酸、甘胺酸、牛磺酸及糖與蒸餾水混合均勻,其實驗反應物使用量配方表如下表4所示,將混合均勻之樣品以100℃恆溫於矽油浴中加熱反應120分鐘,反應結束後放密封冷置一天。經由加熱發生史崔克裂解反應(Strecker degradation)後,以乾燥金針菇為基 質之熱反應香料生成硫化氫、胺和乙醛。這些化合物羰基化合物可進一步形成包括呋喃、吡嗪、噻唑、噻吩等雜環化合物。 After understanding the aroma components of the dried Flammulina velutipes, the preparation of the thermoreactive flavors was followed by aroma analysis. The main preparation process is as shown in Fig. 1 : fresh Flammulina velutipes are air-dried at 35, 40, 45, 50 ° C temperature and stored at this temperature for 5 weeks, respectively, to obtain dried Flammulina velutipes. The dried Flammulina velutipes, arginine, alanine, glycine, taurine and sugar are mixed uniformly with distilled water. The formula of the experimental reactant usage is shown in Table 4 below, and the uniformly mixed sample is kept at 100 ° C in the eucalyptus oil. The reaction was heated in a bath for 120 minutes, and after the reaction was completed, the seal was allowed to stand for one day. After Strecker degradation occurs via heating, based on dried Flammulina velutipes The hot reaction fragrance produces hydrogen sulfide, amine and acetaldehyde. These compound carbonyl compounds can further form heterocyclic compounds including furan, pyrazine, thiazole, thiophene and the like.
製備得熱反應香料後,同實施例1,進行喜好性官能 品評試驗法,對於以不同條件處理之乾燥金針菇為基質與不同酸鹼值所製備的熱反應香料作冬瓜風味(Dong gua Flavor)、海產風味(Seafood Flavor)、堅果風味(Nuts Flavor)、香菇風味(Mushrooms Flavor)等四個項目,依其喜好程度進行品評,品評結果如表5所示。 After preparing the heat-reactive fragrance, in the same manner as in Example 1, the preferred function was carried out. Tasting test method for Donggaya Flavor, Seafood Flavor, Nuts Flavor, and Mushroom Flavor (Mushrooms Flavor) and other four projects, according to their preference for evaluation, the results of the evaluation are shown in Table 5.
如表5所示,以未添加乾燥金針菇所製備而成之熱反 應香料具有濃郁的冬瓜風味;在以35℃及40℃乾燥及儲藏5周之乾燥金針菇為基質之熱反應香料的組別中,則是具有較明顯堅果風味及些微香菇風味。最後,45℃及50℃組別又依照酸鹼值之不同 而有差異:酸鹼值為5-6時,45℃及50℃組別之熱反應香料在呈現風味上兼具海產風味及堅果風味;酸鹼值為7-8時,45℃及50℃組別之熱反應香料則以濃郁海產風味呈味。 As shown in Table 5, the heat reversed by the addition of dried Flammulina velutipes The flavor should have a rich melon flavor; in the group of heat-reactive flavors of dried Flammulina velutipes dried at 35 ° C and 40 ° C for 5 weeks, it has a more pronounced nutty flavor and a slightly mushroom flavor. Finally, the 45 ° C and 50 ° C groups are different according to the pH value. There are differences: when the acid-base value is 5-6, the heat-reactive fragrances in the 45°C and 50°C groups have both seafood flavor and nut flavor; the acid-base value is 7-8, 45°C and 50°C. The hot reaction flavors of the group are flavored with a rich seafood flavor.
延續實施例3之熱反應香料,挑選較具有濃郁海產風味之組別:45℃及50℃乾燥並儲藏至第5周的乾燥金針菇以酸鹼值7或8條件下產生的熱反應香料(F45-7、F45-8、F50-7及F50-8)及未添加乾燥金針菇的空白組(Blank),利用水蒸氣蒸餾與溶劑萃取法,萃取其香氣物質,並進行鑑定。其結果分別於45℃組別及50℃組別鑑定出49種及64種揮發性成分,包含醇、醛、酮類、酯類、酸類、氮氫化合物和雜環類等香氣化合物,相較於實施例2之乾燥金針菇材料相比增加2-3倍以上之香氣化合物。不僅如此,如表6及表7所示,具有海產香氣相關之化合物種類亦增加,包含:以上結果與實施例4之感官品評可相互對應。 The heat-reactive flavor of Example 3 was continued, and a group having a richer seafood flavor was selected: a heat-reactive flavor produced by drying the Flammulina velutipes at 45 ° C and 50 ° C to the 5th week under the condition of pH 7 or 8 (F45) -7, F45-8, F50-7 and F50-8) and the blank group (Blank) without adding dried Flammulina velutipes, the aroma substances were extracted by steam distillation and solvent extraction, and identified. The results showed that 49 kinds and 64 kinds of volatile components were identified in the 45 °C group and the 50 °C group, including aroma compounds such as alcohols, aldehydes, ketones, esters, acids, nitrogen compounds and heterocyclic compounds. The aroma compound was added 2-3 times or more to the dried Flammulina velutipes material of Example 2. Moreover, as shown in Tables 6 and 7, the types of compounds related to aroma of marine products also increased, including: the above results correspond to the sensory evaluations of Example 4.
依據以上說明,本發明突破了以往以化學合成人工香料之製作方法,改以利用具有健康營養價值之天然植物作為純素食又具有海產葷食風味之熱反應香料,不僅提供一天然安全之素食加工食品調味香料,使得純素食者可享用健康與風味兼具之素食加工食品,同時亦解決了金針菇保鮮期短不易存放之問題。 According to the above description, the present invention breaks through the conventional method of chemically synthesizing artificial flavor, and uses a natural plant having healthy and nutritious value as a vegan food and a hot reaction flavor having a seafood foraging flavor, which not only provides a natural and safe vegetarian processing. Food flavoring and fragrance, so that vegans can enjoy vegetarian and processed foods with both health and flavor, and also solve the problem that the sweet-keeping period of Flammulina velutipes is short and difficult to store.
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