TWI572292B - A Method for Preparing Beef Flavor with Black Fructus as Base - Google Patents

A Method for Preparing Beef Flavor with Black Fructus as Base Download PDF

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TWI572292B
TWI572292B TW104123103A TW104123103A TWI572292B TW I572292 B TWI572292 B TW I572292B TW 104123103 A TW104123103 A TW 104123103A TW 104123103 A TW104123103 A TW 104123103A TW I572292 B TWI572292 B TW I572292B
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acid
beef flavor
beef
vegetarian
flavor
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TW201703651A (en
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Li Yun Lin
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Hungkuang Univ
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以黑木耳為基質之素牛肉香料及其製備方法 Black fungus-based beef flavor and preparation method thereof

本發明係關於一種以乾燥黑木耳為基質之具有牛肉風味的素食肉味香料,及其製備方法。尤其,本發明係關於一種以經過乾燥之食用菇(黑木耳)為基質,經熱反應處理而得之牛肉風味香料,以及其應用於食品調味與添加之組成物。 The invention relates to a vegetarian meat flavored spice with beef flavor based on dried black fungus and a preparation method thereof. In particular, the present invention relates to a beef flavored spice obtained by heat-treating a dried edible mushroom (black fungus) as a substrate, and a composition thereof for seasoning and adding food.

香味係食品美味的重要指標,現代加工食品常因為在加工過程中會造成食品中香氣成分損失減弱,而使得消費者選購加工食品之意願降低。因此,香料便成為加工食品不可或缺之添加元素,其中,又以肉味香料為香料領域的研究重點。如日本專利申請號第2003-167302號,係有關一種製造具有討喜的似烤肉味的氣味和味道的調味料之方法;而南韓專利申請號第10-2013-0086953號,係關於一種用於製備天然牛肉味之方法等。肉味香料亦已廣泛應用於速食食品、膨化休閒食品、冷凍調理、肉品加工及醬料調理等產品中。 Aroma is an important indicator of food deliciousness. Modern processed foods often reduce the loss of aroma components in foods during processing, which makes consumers less willing to purchase processed foods. Therefore, spices have become an indispensable additive element for processed foods, among which meat flavors are the focus of research in the field of spices. For example, Japanese Patent Application No. 2003-167302 relates to a method for producing a seasoning having a pungent taste and taste similar to that of a barbecue; and the Korean Patent Application No. 10-2013-0086953 relates to a method for A method of preparing a natural beef flavor, and the like. Meat flavors have also been widely used in fast food, puffed snack foods, frozen conditioning, meat processing and sauce conditioning.

肉味香料大多係以動物性肉類作為香料之來源,隨著近年素食文化以及養生風氣漸長,食品業者越來越重視素食產品之開發。由於全素肉香料不能以肉類或辛香植物作為反應原料,因此於製備時在原料方面有較少的選項,且就技術層面而言 也較為困難,故許多業者選擇直接使用人工化學合成香料做為素食食品之肉味調味來源。 Most of the meat flavors are based on animal meat as a source of spices. With the growing vegetarian culture and health culture in recent years, food industry players are paying more and more attention to the development of vegetarian products. Since vegan flavours cannot be used as a raw material for meat or spice plants, there are fewer options in terms of raw materials at the time of preparation, and technically speaking It is also more difficult, so many operators choose to use artificial chemical synthetic fragrance directly as a source of meat flavoring for vegetarian food.

然而,因現代人們對於健康食品安全的意識抬頭,不同於以往傳統使用人工化學合成香料之調味方法,食品加工業者與食科研究人員開始著重於開發以天然食材製備具有肉香風味之素肉香料。如中華民國專利申請號第96134427號,其發明係以香椿製作肉精風味之調味料的方法、製成品及其應用,乃係利用天然植物香椿以乳酸菌發酵技術處理,並加熱製成具有肉香味之調味料,可適用於純素食食品中。 However, due to modern people's awareness of health food safety, unlike the traditional methods of using artificial chemical synthetic flavors, food processors and food researchers have begun to focus on the development of meat flavors with natural flavors. . For example, in the Republic of China Patent Application No. 96134427, the invention relates to a method, a finished product and an application thereof for preparing a seasoning of a meat flavor flavor by using a natural plant toxin, which is treated by a lactic acid bacteria fermentation technique and heated to have a meat flavor. Seasonings are suitable for use in vegan foods.

為兼顧符合食品安全要求及純素食者對於食品調味美味之要求,本發明遂利用乾燥黑木耳作為基材,利用加熱處理技術開發具有牛肉風味的素食肉味香料。 In order to meet the requirements of food safety requirements and vegan foods for flavoring and flavoring, the present invention utilizes dried black fungus as a substrate to develop a beef flavored vegetarian meat flavoring by heat treatment.

黑木耳(Auricularia auricula-judae)又稱為雲耳,係木耳科、木耳屬,英文為wood/cloud ear mushroom/fungus,係一種普遍的食用菇類,其口感爽脆其內含之營養價值極高,富含豐富植物膠原及纖維素,可有效促進胃腸蠕動;含鈣及鐵含量高,具有涼血止血之作用,亦可改善貧血之症狀;自其中提取之酸性多醣成分更具有抗血栓的功能。 Auricularia auricula-judae , also known as the cloud ear, is a fungus, a fungus, and a wood/cloud ear mushroom/fungus in English. It is a popular edible mushroom. Its taste is crisp and its nutritional value is extremely high. High, rich in plant collagen and cellulose, can effectively promote gastrointestinal motility; high calcium and iron content, with cooling blood to stop bleeding, can also improve the symptoms of anemia; the acid polysaccharide extracted from it is more antithrombotic Features.

於中華民國專利申請號第096107824號,係一種含黑木耳成分之食品及其製法,其係將黑木耳乾燥後,再經細微化處理所製得的黑木耳粉粒進一步與主料組份相混合。主要能透過其製法而增加黑木耳所內含之營養成分吸收攝取的效果及食用之方 便性。先前技術皆未曾揭示以黑木耳作為熱反應香料的基材,而本發明係首先研發以乾燥黑木耳為基材,經由加熱反應後可產生具有牛肉風味之香氣,進而將所得之熱反應香料應用於食品調味與添加之組成物。 Patent Application No. 096107824 of the Republic of China, which is a food containing black fungus component and a method for preparing the same, which is a black fungus powder obtained by drying the black fungus and then subjected to miniaturization treatment, and further reacting with the main ingredient component. mixing. It can mainly increase the absorption and ingestion of nutrients contained in black fungus and the side of consumption through its preparation method. Convenience. The prior art has not disclosed a substrate using black fungus as a heat-reactive fragrance, and the present invention firstly developed a dry black fungus as a substrate, and after heating, a flavor having a beef flavor can be produced, and the resulting heat-reactive fragrance can be applied. A composition that is flavored and added to food.

鑒於上述之目的,本發明係利用食用菇-黑木耳,將其乾燥後做為基質,經過加熱反應製成具有牛肉風味的香料,可應用於素食或葷食食品加工做為提增牛肉味香氣的全素天然添加物。 In view of the above purposes, the present invention utilizes edible mushroom-black fungus, which is dried and used as a substrate, and is heated to produce a flavor having beef flavor, which can be applied to vegetarian or foraging food processing as a flavor of beef flavor. A vegan natural additive.

因此,於一方面,本發明係關於一種素牛肉香料,其特徵在於以乾燥黑木耳為基材,與維生素、水解植物蛋白、木糖、胺基酸及脂肪酸經過80℃~120℃加熱反應而產生者。於本發明之某些具體實施例,其中該乾燥黑木耳之水分含量係介於8~15%。於本發明之一項具體實施例,其中該維生素係維生素B1。於本發明之其他某些具體實施例,其中該胺基酸係選自甲硫胺酸(Methionine)及半胱胺酸(Cystine)至少任一者。 Therefore, in one aspect, the present invention relates to a vegetarian beef flavor characterized in that the dried black fungus is used as a substrate, and the vitamin, the hydrolyzed vegetable protein, the xylose, the amino acid and the fatty acid are heated and reacted at 80 ° C to 120 ° C. Producer. In some embodiments of the invention, the moisture content of the dried black fungus is between 8 and 15%. In one particular embodiment of the present invention, wherein the vitamin-based vitamin B 1. In some other specific embodiments of the invention, the amino acid is selected from at least one of Methionine and Cystine.

另一方面,本發明提供一種包含前述之素牛肉香料的食品調味組成物。 In another aspect, the present invention provides a food flavoring composition comprising the aforementioned beef flavor.

又另一方面,本發明亦提供本發明之素牛肉香料的製備方法,其包含:將乾燥黑木耳與維生素、水解植物蛋白、木糖、酸基酸及脂肪酸混合,並調整該混合物之pH值為pH 5.0-8.0;及將該混合物於80℃~100℃加熱30~60分鐘,而製得素牛肉香料。 In still another aspect, the present invention also provides a method for preparing the vegetarian beef flavor of the present invention, comprising: mixing dry black fungus with vitamins, hydrolyzed vegetable protein, xylose, acid acid and fatty acid, and adjusting the pH of the mixture. The pH is 5.0-8.0; and the mixture is heated at 80 ° C to 100 ° C for 30 to 60 minutes to obtain a vegetarian beef flavor.

於本發明之一項具體實施例,其中該維生素係維生素B1。於本發明之其他某些具體實施例,其中該胺基酸係選自甲硫胺酸(Methionine)及半胱胺酸(Cystine)至少任一者。 In one particular embodiment of the present invention, wherein the vitamin-based vitamin B 1. In some other specific embodiments of the invention, the amino acid is selected from at least one of Methionine and Cystine.

於本發明之一項具體實施例,所述之製備方法其中進一步包含將素牛肉香料加工製成膏狀或粉末狀。 In a specific embodiment of the present invention, the preparation method further comprises processing the vegetarian beef flavor into a paste or a powder.

第1圖 素牛肉香料之製備方法 Figure 1 Preparation method of vegetarian beef flavor

雖然本發明可以用不同形式之實施例呈現,但附圖所示者及於以下實施例說明係本發明之較佳實施範例,請瞭解本文中所揭示僅為本發明之輔助說明,並非係以意圖用以限制本發明之範圍。 While the invention may be embodied in various embodiments, the embodiments illustrated in the drawings It is intended to limit the scope of the invention.

本發明係關於一種以黑木耳為基質之素牛肉香料及其製備方法,且可應用於食品調味上。其中具牛肉風味的素食肉味香料該製備方法如第1圖所示,主要為:將乾燥黑木耳與維生素、水解植物蛋白、木糖、胺基酸及脂肪酸混合;將該混合物於80℃~120℃加熱30~60分鐘,而成具有牛肉香氣之熱反應香料。 The invention relates to a black fungus-based beef flavor and a preparation method thereof, and can be applied to food seasoning. The preparation method of the beef flavored beef flavor is as shown in Fig. 1, mainly: mixing the dried black fungus with vitamins, hydrolyzed vegetable protein, xylose, amino acid and fatty acid; the mixture is at 80 ° C~ Heat at 120 ° C for 30 to 60 minutes to form a hot reaction flavor with beef aroma.

實施例1 Example 1

首先,將不同濃度(A-1:6.25g/L、A-2:10g/L、 A-3:18.75g/L、A-4:25g/L、A-5:31.25g/L)之乾燥黑木耳粉末,與脂肪酸肉豆蔻酸、棕櫚酸、硬脂酸、油酸等脂肪酸、300g/L植物水解蛋白、5g/L木糖(Xylose)、5g/L半胱胺酸(Cys)及7.5g/L維生素B1混合均勻後,於100℃進行加熱反應1小時,即製成素牛肉反應液。 First, different concentrations (A-1: 6.25 g/L, A-2: 10 g/L, A-3: 18.75 g/L, A-4: 25 g/L, A-5: 31.25 g/L) Dry black fungus powder, fatty acids such as fatty acid myristic acid, palmitic acid, stearic acid, oleic acid, 300g/L hydrolyzed protein, 5g/L xylose, 5g/L cysteine (Cys) and After 7.5 g/L of vitamin B 1 was uniformly mixed, the reaction was heated at 100 ° C for 1 hour to prepare a vegetarian beef reaction solution.

將前述之素牛肉反應液利用固相微萃法吸附,並以氣相層析質譜儀(Gas Chromatography,GC-MS)進行揮發性化合物分析。經由與值譜的資料庫比對分析後,一共鑑定出34種揮發性化合物,依照官能基分類主要可分為5大類,分別為:醛類6種、碳氫化合物8種、醇類及酮類3種、含硫化合物8種以及雜環類化合物9種。將以上結果和各類已知與牛肉香氣有關之香氣化合物進一步進行比對分析,其分析如下表1所示。 The above-mentioned vegetarian beef reaction solution was adsorbed by solid phase microextraction, and volatile compound analysis was carried out by gas chromatography mass spectrometry (Gas Chromatography, GC-MS). After comparison with the database of the value spectrum, a total of 34 volatile compounds were identified. According to the functional group classification, they can be divided into five major categories: 6 aldehydes, 8 hydrocarbons, alcohols and ketones. There are three types, eight kinds of sulfur-containing compounds, and nine kinds of heterocyclic compounds. The above results were further subjected to comparative analysis with various aroma compounds known to be related to beef aroma, and the analysis thereof is shown in Table 1 below.

如表1所示,於素牛肉反應液中共鑑定出15種與牛肉香氣相關之香氣化合物,分別為醛類4種、碳氫化合物2種、醇類及酮類1種、含硫化合物4種以及雜環類化合物4種。其中,含硫化合物為熱反應之重要產物,是使得素牛肉反應液具有牛肉香氣之主要貢獻者;另外雜環類之吡嗪(Pyrazine)化合物對也是主要形成牛肉香氣來源之一,主要提供燒烤的味道。依據表1結果進一步分析後,由乾燥黑木耳所製成的素牛肉反應液相似於牛肉香氣平均約為46.82%,另外,又以濃度於A-3時所製成之素牛肉反應液具49.49%與牛肉香味最為近似,而最低濃度A-1近似牛肉香味之程度 則為45.72%。 As shown in Table 1, 15 kinds of aroma compounds related to beef aroma were identified in the reaction solution of beef, which were 4 kinds of aldehydes, 2 kinds of hydrocarbons, 1 kind of alcohols and ketones, and 4 kinds of sulfur compounds. And four kinds of heterocyclic compounds. Among them, the sulfur-containing compound is an important product of the thermal reaction, which makes the vegetarian beef reaction liquid have the main contributor of beef aroma; in addition, the heterocyclic pyrazine compound pair is also one of the main sources of beef aroma, mainly providing barbecue. the taste of. After further analysis according to the results in Table 1, the vegetarian beef reaction liquid prepared from the dried black fungus was similar to the beef aroma average of about 46.82%, and the vegetarian beef reaction solution prepared at a concentration of A-3 was 49.49. % is most similar to beef flavor, and the lowest concentration A-1 is similar to the degree of beef flavor. It is 45.72%.

實施例2 Example 2

為了做出最為近似牛肉風味之素牛肉香料,遂進一步用乾燥黑木耳於不同pH值、時間與溫度下,以不同條件進行素牛肉香料之製備,以找到最適合之製備條件。以18.75g/L乾燥黑木耳為基質,與脂肪酸肉豆蔻酸、棕櫚酸、硬脂酸、油酸等脂肪酸、300g/L植物水解蛋白、5g/L木糖(Xylose)、5g/L半胱胺酸(Cys)及7.5g/L維生素B1混合均勻後,於不同pH值(5、6、7及8)、不同加熱時間(30、40、50及60分鐘)及不同溫度(90、100、110及120℃)下,製備素牛肉反應液。以固相微萃取法吸附揮發性化合物並以GC-MS進行分析,並鑑定與牛肉香氣相關之香氣化合物所占之百分比進行比較,其實驗結果如下表2所示。 In order to make the beef flavor that is most similar to beef flavor, the dried black fungus is further prepared at different pH values, time and temperature under different conditions to find the most suitable preparation conditions. 18.75g / L dry black fungus as a substrate, with fatty acids such as myristic acid, palmitic acid, stearic acid, oleic acid, 300g / L plant hydrolyzed protein, 5g / L xylose (Xylose), 5g / L cysteine After mixing the amino acid (Cys) and 7.5g/L vitamin B 1 at different pH values (5, 6, 7 and 8), different heating times (30, 40, 50 and 60 minutes) and different temperatures (90, The vegetarian beef reaction solution was prepared at 100, 110 and 120 ° C). Volatile compounds were adsorbed by solid phase microextraction and analyzed by GC-MS, and the percentage of aroma compounds related to beef aroma was identified. The experimental results are shown in Table 2 below.

如表2所示,以pH值比較素牛肉反應液之牛肉風味程度,可觀察到當pH值偏於弱酸性(pH5-6)進行熱反應處理時,其素牛肉反應液之牛肉風味呈現百分比較高,若於偏弱鹼性則其牛肉味香氣較差;以不同溫度進行加熱處理之結果觀察可發現,用90-100℃相較於110-120℃加熱反應之牛肉香味程度較高;最後,雖然以加熱時間並無法發現出規律變化,但利用針對前兩類較佳條件分類,於pH值5-6以及90-100℃之製備條件下,以加熱30分鐘時所製備成的素牛肉反應液,其牛肉香氣最為近似(71.99%)。 As shown in Table 2, the beef flavor of the vegetarian beef reaction solution was compared with the pH value, and the percentage of beef flavor of the vegetarian beef reaction liquid was observed when the pH value was weakly acidic (pH 5-6) for thermal reaction treatment. Higher, if it is weakly alkaline, its beef flavor is poor; the results of heat treatment at different temperatures can be found that the beef flavor is higher when heated at 90-100 °C compared to 110-120 °C; Although the regular change can not be found by the heating time, the prepared beef is prepared by heating for 30 minutes under the preparation conditions of pH 5-6 and 90-100 ° C according to the first two types of preferred conditions. The reaction liquid has the most similar beef aroma (71.99%).

綜合以上結果,證實本發明之乾燥黑木耳可與維生素、水解植物蛋白、糖類、胺基酸及脂肪酸混合後,經由加熱反應,而製備成具有牛肉香氣之肉味香料。尤其,本發明之素牛肉香料係以乾燥黑木耳為基材所製備而成之純素食香料,不僅可應用於純素食之食品中提增其食品牛肉香氣,營養價值亦高。因此, 本發明可同時兼顧素食者對於素食之風味要求與調味香料之食品安全,係為純素肉味香料之一大突破。 Based on the above results, it was confirmed that the dried black fungus of the present invention can be mixed with vitamins, hydrolyzed vegetable protein, sugar, amino acid, and fatty acid, and then reacted by heating to prepare a meaty flavor having beef aroma. In particular, the vegetarian beef flavor of the present invention is a pure vegetarian flavor prepared by drying black fungus as a base material, and can be used not only in vegan foods, but also in enhancing the aroma of the food beef and having high nutritional value. therefore, The invention can simultaneously take into consideration the food safety requirements of the vegetarians for the flavor of the vegetarian food and the flavoring of the flavoring, and is a breakthrough in the vegan meat flavoring.

Claims (7)

一種素牛肉香料,其特徵在於係以乾燥黑木耳為基材,與維生素B1、水解植物蛋白、木糖(Xylose)、胺基酸及脂肪酸於80℃~120℃加熱30~60分鐘進行加熱反應而產生者;其中該胺基酸係選自甲硫胺酸(Methionine)及半胱胺酸(Cystine)至少任一者;其中該脂肪酸係為脂肪酸肉豆蔻酸、棕櫚酸、硬脂酸、油酸。 A vegetarian beef flavor characterized by drying black fungus as a substrate, heating with vitamin B 1 , hydrolyzed vegetable protein, xylose, amino acid and fatty acid at 80 ° C to 120 ° C for 30 to 60 minutes. Producing the reaction; wherein the amino acid is selected from at least one of Methionine and Cystine; wherein the fatty acid is fatty acid myristic acid, palmitic acid, stearic acid, Oleic acid. 如請求項1所述之素牛肉香料,其中該乾燥黑木耳之水分含量係介於8~15%。 The vegetarian beef flavor according to claim 1, wherein the dried black fungus has a moisture content of 8 to 15%. 一種食品調味組成物,其係包含如請求項1所述之素牛肉香料者。 A food flavoring composition comprising the vegetarian beef flavor as claimed in claim 1. 一種如請求項1所述之素牛肉香料的製備方法,其包含:將乾燥黑木耳與維生素B1、水解植物蛋白、木糖、胺基酸及脂肪酸混合,並調整該混合物之pH值為pH 5.0-8.0;及將該混合物於80℃~120℃加熱30~60分鐘,而製得素牛肉香料;其中該胺基酸係選自甲硫胺酸(Methionine)及半胱胺酸(Cystine)至少任一者;其中該脂肪酸係為肉豆蔻酸、棕櫚酸、硬脂酸、油酸。 A method for preparing a vegetarian beef flavor according to claim 1, which comprises mixing dry black fungus with vitamin B 1 , hydrolyzed vegetable protein, xylose, amino acid and fatty acid, and adjusting the pH of the mixture to pH. 5.0-8.0; and heating the mixture at 80 ° C ~ 120 ° C for 30 ~ 60 minutes to obtain a vegetarian beef flavor; wherein the amino acid is selected from the group consisting of Methionine and Cystine At least one of the fatty acids is myristic acid, palmitic acid, stearic acid, oleic acid. 如請求項4所述之製備方法,其中該混合物之pH值為pH 5.0-6.0。 The preparation method according to claim 4, wherein the pH of the mixture is pH 5.0 to 6.0. 如請求項4所述之製備方法,其中該混合物係於90-100℃加熱30~40分鐘。 The preparation method according to claim 4, wherein the mixture is heated at 90 to 100 ° C for 30 to 40 minutes. 如請求項4所述之製備方法,其中進一步包含將素牛肉香料加工製成膏狀或粉末狀。 The preparation method according to claim 4, which further comprises processing the vegetarian beef flavor into a paste or a powder.
TW104123103A 2015-07-16 2015-07-16 A Method for Preparing Beef Flavor with Black Fructus as Base TWI572292B (en)

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Title
李江彬,"以熱反應生產牛肉香料之研究",弘光科技大學食品科技研究所,碩士論文,2014/11/21 *

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