TW201538718A - Method for solid state fermentation of potent medicinal fungi using fully-functioning solid state fermentation system - Google Patents

Abstract

A method for solid state fermentation of potent medicinal fungi using fully-functioning solid state fermentation system is implemented by integrating production processes of sterilization, bacteria planting, cultivating, drying and harvesting. In this invention, cultivation trays are loaded with culture medium, and placed on the movable tiers inside the reaction tank for solid state fermentation in a tray culture manner. Further, internal pipeline is used to provide planting bacteria, liquid nutrient and gas to supply the nutrient needed for microbial fermentation. In addition, the tank body can be provided with a drying cycle unit together with a product harvesting unit to proceed with drying and harvesting processes, respectively. By this way, it can effectively integrate the production process of solid state fermentation. During the process, there is no need for the fermentates to transfer machine, which can greatly reduce the pollution probability in the process of production and is not necessary to purchase highly-mechanized automated production equipments. This invention can reduce the demand for operation manpower, and can be in extremely low production cost by adopting a simple tank body design and production steps. Moreover, this invention can carry out mass production in high efficiency, thereby achieving the effects of time and energy saving, smooth production processes, low cost, simple operation, low manpower needs and low pollution, as well as achieving automatic production goal without requiring automated production equipments.

Description

Method for solid-state fermentation of full-function high-efficiency edible and medicinal solid-state fermentation system

The invention relates to a method for solid-state fermentation of a full-function high-efficiency edible and medicinal solid-state fermentation system integrated in a sterilization process of sterilizing, sterilizing, cultivating, drying and harvesting, in particular to save time and energy and produce The process is coherent, low-cost, simple to operate, low manpower requirements, and low pollution, and can achieve the goal of automated mass production without the need for automated production equipment.

At present, the common solid-state fermentation systems can be classified into the following types: The first type is a Gas-Solid Fluidized Bed Fermenter, which is usually an upright tank filled with a solid medium. Use the machine to stir up and down the way, and open the input air of the air pipe to provide air and cool down. The humidity is supplied by water from the top; however, this system is quite energy-consuming, and the matrix is easy to squeeze when stirring. Broken, and its volume is more limited by the height at which the medium can be stacked. The second type is the Rotary Drum Fermenter. These fermenters are mostly cylindrical tanks that can be overturned in a 360-degree rotation, ventilated and liquid nutrients; however, this The volume of the fermentation tank should not exceed one-third, the tank capacity is limited in use, energy consumption and expensive, and the disturbance caused by the rotation process may be detrimental to the growth of the microorganism to be placed, and the medium also has agglomeration. possibility. The third type is the tray fermentation tank (Tray Fermenter), which is filled with the culture medium on the fermenting tray, and the tray is installed on the inner shelf of the fermentation tank to solve the problem of the above-mentioned medium stacking, and the heat dissipation is good. Providing air and liquid nutrients, and providing a good growth environment for microorganisms that need to be allowed to stand for fermentation; however, this method is US Pat. No. 6,620,614 B1, which is labor intensive in installation and recovery, and on the production process. There is still a lack of coherence. Some systems try to coherently design some processes, such as the Republic of China invention patent No. I409329, but the design is overly complicated and expensive.

Therefore, there is currently no simple design in the tray fermentation tank that integrates from sterilization to harvesting.

In view of this, the above-mentioned various literatures show that the fermentation tank designed in the past has problems such as energy consumption, damage to the culture substrate stack, high price, labor consumption, or excessive design complexity, and there is no integrated design process. Therefore, the user-like users cannot meet the needs of the user in actual use.

The main object of the present invention is to overcome the above problems encountered in the prior art and to provide an easy-to-operate production process and a simple tank design, which greatly reduces manpower requirements, saves time and energy, and reduces the cost of purchasing automation equipment. The inconsistent solid-state fermentation process is combined with a solid-state fermentation system that can produce a large number of high-efficiency edible and medicinal bacteria solid-state fermentation systems that can be produced in large quantities and low in pollution.

For the purpose of the above, the present invention is a method for solid-state fermentation of a full-function high-efficiency edible medicinal bacterium solid-state fermentation system, which comprises at least the following steps: (A) providing a full-function solid-state fermentation device integrated with a solid production process. The utility model comprises a reaction tank, the side of which is provided with an observation window, and the front surface is provided with a slit cover; a movable shelf disposed in the reaction tank, the bottom of which is hollowed out and has more than one layer of the culture plate At least one culture tray placed on the movable shelf of the bottom hollow, each culture tray is stacked on a culture tray placement layer, and each culture tray has a chassis; one is disposed in the reaction tank The liquid internal pipeline comprises a liquid pipeline and a drainage hole, the liquid pipeline has a liquid pipeline inlet, and a plurality of spray pipelines are connected to the liquid pipeline, and each spray pipeline is disposed above each culture tray placement layer a gas internal pipeline disposed in the reaction tank, comprising a gas line and an gas outlet, the gas pipeline having a gas line inlet port, and the gas pipe a plurality of bronchial lines are connected, each bronchial line is disposed between each of the culture plate placement layers; and a product recovery unit includes a collection tray disposed in the reaction tank and inclined inwardly like a funnel (B) providing a plurality of media, each medium is filled on a culture tray; (C) introducing a liquid into the liquid line through the inlet of the liquid line and diverting to each spray line, by uniformly spraying the liquid Liquid germination or liquid feeding is provided on each layer of the culture tray to supply the liquid nutrients required for the fermentation of the bacterium and the microorganism, and the excess liquid is discharged from the drainage hole below the reaction tank; (D) Gas is introduced into the gas line through the gas inlet port of the gas line and is branched to each bronchial line, and provides aerobic gas and temperature control and heat dissipation by diffusing the gas to each of the culture plates to supply the microorganisms for fermentation. Oxygen is discharged from the gas outlet above the reaction tank; and (E) the fermentation and microbial growth conditions are observed through the observation window, and after the fermentation is completed, Drying, drying medium was collected through the collection tray, thereby obtaining a fermentation product.

In the above embodiment of the present invention, the reaction tank is integrally formed, and the outer shape of the tank may be cylindrical or square.

In the above embodiment of the present invention, the reaction tank is an active tank body having a wheel that can be moved to a tunnel sterilizer for sterilization.

In the above embodiment of the present invention, the reaction tank is a fixed tank body, which is a pressure vessel formed by a reinforced structure, and can be directly sterilized by steam in situ.

In the above embodiment of the present invention, the spray lines are detachably connected to the liquid line, and the bacteria are implanted by means of a sprinkler head or a sprayer, or may be used as an action of adding a liquid feed.

In the above embodiment of the present invention, the method further includes a drying cycle unit, wherein the front surface of the reaction tank and the opposite tank are respectively provided with a hot dry gas inlet and a hot dry gas outlet, and a front cover of the reaction tank The opposite tank is provided with a plurality of hot gas dispersion ports, and each of the hot gas dispersion ports corresponds to at least one culture disk placement layer for providing uniform hot gas dispersion to each of the culture disk placement layers.

In the above embodiment of the present invention, when the reaction tank body is a pressure vessel, the outlet of the hot dry gas may be connected to a vacuum exhaust pipe for vacuum drying.

In the above embodiment of the invention, the drying cycle unit replaces the gas internal line as needed.

In the above embodiment of the present invention, the product recovery unit further comprises a harvesting bucket, and the culture tray placed on the movable shelf, the chassis of which can be extracted from the front side of the culture tray, and the dried medium can be Drop onto the collection tray and collect it into the collection bin.

100‧‧‧ Full-function solid-state fermentation unit 10‧‧‧Reaction tank 11‧‧‧ Observation window 12‧‧‧Slot cover 13‧‧‧ Wheels 20‧‧‧ Activity shelf 21‧‧‧Crystal placement layer 30‧ ‧‧Cultivities 31‧‧‧Chassis 40‧‧‧Liquid internal pipeline 41‧‧‧Liquid line 42‧‧Drain hole 43‧‧‧Liquid line inlet 44‧‧‧Spray line 50‧‧‧ Gas internal pipeline 51‧ ‧ ‧ gas pipeline 52‧‧ ‧ air outlet 53‧ ‧ gas line inlet 54‧ ‧ bronchial line 60 ‧ ‧ dry cycle unit 61 ‧ ‧ hot dry gas inlet 62 ‧ ‧ hot dry gas outlet 63 ‧ ‧Hot gas dispersion port 70‧‧‧Product recovery unit 71‧‧‧Collection tray 72‧‧‧ Harvesting barrel 1‧‧‧ medium

Fig. 1 is a schematic view showing the front structure of the apparatus of the present invention.
Fig. 2 is a schematic view showing the side structure of the apparatus of the present invention.
Fig. 3 is a schematic view showing the extraction of the tray of the present invention.

Please refer to FIG. 1 to FIG. 3, which are schematic diagrams showing the front structure of the device of the present invention, the side structure of the device of the present invention, and the schematic diagram of the tray of the present invention. As shown in the figure: the present invention is a solid-state fermentation method of a full-function high-efficiency edible and medicinal fungus solid-state fermentation system, which can be used for providing solid-state fermentation production such as sterilization, phytobacteria, culture, drying and harvesting. The process includes at least the following steps:

(A) A full-function solid-state fermentation apparatus 100 is provided which integrates a production process such as sterilization, sterilizing, culturing, drying and harvesting, and comprises a reaction tank 10, a observation window 11 is arranged on the side, and a front surface is provided. a slit cover 12; a movable shelf 20 disposed in the reaction tank 10, the bottom of which is hollowed out and having more than one culture tray placement layer 21; at least one movable shelf placed at the bottom hollow 20, the culture tray 30 is stacked on a culture tray placement layer 21, and each culture tray 30 has a chassis 31; a liquid internal pipeline 40 disposed in the reaction tank 10, including a liquid line 41 and a drain hole 42 having a liquid line inlet 43 and a plurality of spray lines 44 connected to the liquid line 41, each spray line 44 being disposed on each of the culture trays Above the 21; a gas internal pipeline 50 disposed in the reaction tank 10, comprising a gas line 51 and an air outlet 52, the gas The pipeline 51 has a gas line inlet 53 and a plurality of bronchial lines 54 connected to the gas line 51. Each bronchial line 54 is disposed between each of the culture tray placement layers 21; a drying cycle unit 60 includes A heat dry gas inlet 61 and a hot dry gas outlet 62 are respectively disposed on the front tank cover 12 and the opposite tank body of the reaction tank 10, and a plurality of hot gas dispersion ports 63 are disposed on the front tank cover 12 and the opposite tank body of the reaction tank 10. Each hot gas dispersion port 63 corresponds to at least one culture disk placement layer 21 to form a good drying cycle system, and the drying process can be performed after hot dry air is passed; and a product recovery unit 70 includes a collecting tray 71 disposed in the reaction tank 10 with a bottom portion inclined inwardly like a funnel, and a collecting barrel 72 disposed at the outer bottom of the reaction tank 10;

(B) providing a plurality of mediums 1 and filling each medium 1 on a culture tray 30;

(C) introducing a liquid into the liquid line 41 through the liquid line inlet 43 and diverting it to each spray line 44, providing liquid germination or adding a liquid feed by uniformly spraying the liquid onto each of the culture trays 30, To supply the liquid nutrients required for the fermentation of the bacteria and the microorganisms, and to discharge the excess liquid from the drainage holes 42 in the lower part of the reaction tank 10;

(D) introducing a gas into the gas line 51 through the gas line inlet port 52 and diverting it to each bronchial line 54 to provide aerobic gas and temperature control and dissipating heat by diffusing the gas to each layer of the culture tray 30. To supply oxygen required for microbial fermentation, and is discharged from the gas outlet 52 above the reaction tank 10;

(E) The fermentation and the growth state of the microorganisms are examined through the observation window 11, and dried after the completion of the fermentation, and the dried medium 1 is collected through the collection tray 71, thereby obtaining a fermentation product. If so, the method of the above disclosure constitutes a new method for solid-state fermentation of a full-function, high-efficiency edible and medicinal solid-state fermentation system.

The reaction tank 10 mentioned above is integrally formed, and the outer shape of the tank may be cylindrical or square. The reaction tank 10 can be a movable tank body having a wheel 13 and can be moved to a tunnel sterilizer (not shown) for sterilization, as shown in Figures 1 and 2; or the reaction tank can be fixed. The tank body is a pressure vessel formed by a reinforced structure, and can be directly sterilized by steam in situ.

The above-mentioned spray line 44 is carried out by means of a sprinkler head or a sprayer, or can be used as an action of adding a liquid feed, and for the convenience of installation and removal of the movable shelf 20, the spray lines 44 can be easily removed from the liquid line 41.

In the above-mentioned drying cycle unit 60, when the tank of the reaction tank 10 is a pressure vessel, the outlet of the hot dry gas can be connected to a vacuum exhaust pipe (not shown) for vacuum drying.

In a preferred embodiment, as shown in the first and second figures, the full-function solid-state fermentation device 100 is an integrally formed reaction tank 10, and the front side is a trough cover 12, which can be opened; when used, the medium 1 It can be filled on the culture tray 30 before the work platform, and placed on the movable shelf 20 of the bottom hollow, stacked on each of the culture tray placement layers 21, and several layers can be stacked according to requirements, and then the movable shelf can be stacked. 20, together with the culture trays 30, are pushed into the reaction tank 10, and the spray line 44 is installed on the liquid line 41. After the tank cover 12 is covered, the entire reaction tank 10 can be pushed into the tunnel sterilizer (in the figure) Sterilize in the case shown in the figure. After the bacteria are finished, let stand for cooling. A liquid mother tank (not shown) can be connected to the liquid line inlet 43 above the reaction tank 10, and the liquid bacteria are introduced into the liquid line 41, and the spray lines 44 are evenly sprayed to each of the culture trays 30. On the medium 1, excess liquid is discharged from the lower drain hole 42. Then, the whole full-function solid-state fermentation device 100 is pushed into the culture chamber, and the filtered sterile air is introduced into the gas inlet port 53 and discharged from the gas outlet 52 above the reaction tank 10, and the water can be introduced into the gas line 51 during the culture. The air of the air can maintain the humidity in the tank and control the temperature and have a heat dissipation effect, or adjust the gas content according to the aerobic degree of the microorganism to reach the optimum fermentation environment, and the liquid pipeline 41 can be used to add the alkaline nutrient solution to increase the air. The nutrients of the medium 1 are adjusted and the pH is adjusted to achieve the most efficient fermentation conditions. Among them, the fermentation process can be examined by the tank side observation window 11 during the fermentation culture. After the fermentation is completed, the fermentation product does not need to be taken out from the reaction tank 10. The heated dry air can be introduced into the tank through the hot dry gas inlet 61 on the tank cover 12, and the hot dry gas is uniformly distributed by the hot gas dispersion port 63 of each layer. Drying is performed on each of the culture trays 30, and is discharged from the rear hot dry gas outlet 62. After the drying is completed, the reaction tank tank cover 12 is opened, the spray lines 44 are removed, and the bottom plates 31 of the culture trays 30 are sequentially drawn from the front side of the culture tray 30, as shown in FIG. As shown, the dried medium (i.e., the fermented product) can be dropped onto the bottom of the tank, such as a funnel-shaped collection tray 71, and slipped into the recovery tank 72 to complete the harvest.

Therefore, the full-function solid-state fermentation device designed by the invention can effectively cohere the production process of the solid-state fermentation, and construct a simple disc-type fermentation structure, integrating the production processes such as sterilization, phytobacteria, cultivation, drying and harvesting, and the process. There is no need to transfer the fermenting machine, which can greatly reduce the pollution rate in the production process, and it is not necessary to purchase high-mechanized automatic production equipment, which can reduce the manpower of the operation and generate simple tank design and production steps. Very low production cost and high-efficiency mass production, which enables the device to achieve time-saving and energy-saving, coherent production process, low cost, simple operation, low manpower demand, and low pollution, without the need for automated production equipment. Achieve the goal of automated mass production.

In summary, the present invention is a solid-state fermentation method of a full-function high-efficiency edible and medicinal fungus solid-state fermentation system, which can effectively improve the inconsistency of the conventional solid-state fermentation production process, and construct a simple disc-type fermentation structure, and assemble The production processes of sterilization, bacteriology, culture, drying and harvesting can achieve the effects of time saving, continuous production process, low cost, simple operation, low manpower demand and low pollution, and no need for automated production equipment. Achieving the goal of automated mass production, and thus making the invention more progressive, more practical, and more in line with the needs of the user, has indeed met the requirements of the invention patent application, and filed a patent application according to law.

However, the above is only the preferred embodiment of the present invention, and the scope of the present invention is not limited thereto; therefore, the simple equivalent changes and modifications made in accordance with the scope of the present invention and the contents of the invention are modified. All should remain within the scope of the invention patent.

100‧‧‧Full-function solid state fermentation unit

10‧‧‧Reaction tank

11‧‧‧ observation window

13‧‧‧ Wheels

20‧‧‧ activity shelves

21‧‧‧Cultivating plate placement layer

30‧‧‧Cultivities

40‧‧‧Liquid internal pipeline

41‧‧‧Liquid pipeline

42‧‧‧Drainage holes

43‧‧‧Liquid line inlet

44‧‧‧Spray line

50‧‧‧ gas internal pipeline

51‧‧‧ gas pipeline

52‧‧‧air outlet

53‧‧‧ gas pipeline inlet

54‧‧‧bronchial line

70‧‧‧Product harvesting unit

71‧‧‧ Collection tray

72‧‧‧ harvesting barrel

1‧‧‧ medium

Claims (9)

The invention relates to a method for solid-state fermentation of a full-function high-efficiency edible and medicinal fungus solid-state fermentation system, which comprises at least the following steps:
(A) providing a full-featured solid-state fermentation apparatus integrated with a solid-state production process, comprising a reaction tank having a viewing window disposed on a side thereof, and a slit cover provided on the front surface; and being disposed in the reaction tank a movable shelf, the bottom of which is hollowed out and has more than one layer of the culture tray; at least one culture tray placed on the movable shelf of the bottom hollow, each culture tray is stacked on a culture tray And each culture tray has a chassis; a liquid internal pipeline disposed in the reaction tank, comprising a liquid line and a drain hole, the liquid line has a liquid line inlet, and a plurality of lines are connected to the liquid line a spray line, each spray line is disposed above each of the culture tray placement layers; a gas internal pipeline disposed in the reaction tank includes a gas line and an gas outlet, the gas line has a gas line inlet port, And a plurality of bronchial lines are connected to the gas line, each bronchial line is disposed between each of the culture plate placement layers; and a product recovery unit includes a The reaction vessel bottom is inclined inwardly of the funnel-like collection plate;
(B) providing a plurality of media, each of which is filled on a culture plate;
(C) introducing a liquid into the liquid line through the inlet of the liquid line and diverting it to each spray line, providing liquid germination or adding a liquid feed to uniformly supply the bacterium by uniformly spraying the liquid on each of the culture plates The liquid nutrients required for the fermentation of the microorganisms, and the excess liquid is discharged from the drainage holes below the reaction tank;
(D) introducing a gas into the gas line through the gas inlet port of the gas line and diverting it to each bronchial line, providing aerobic gas and temperature control and dissipating heat by diffusing the gas to each layer of the culture plate to supply the microorganism The oxygen required for fermentation is discharged from the gas outlet above the reaction tank; and (E) the fermentation and microbial growth conditions are examined through the observation window, and dried after the fermentation is completed, and the dried medium is collected through the collection. The tray is collected to thereby obtain a fermented product. The method for solid-state fermentation according to the full-function high-efficiency edible and medicinal solid-state fermentation system described in claim 1, wherein the reaction tank is integrally formed, and the outer shape of the tank can be cylindrical or square. The method for solid-state fermentation according to the full-function high-efficiency edible and medicinal solid-state fermentation system described in claim 1, wherein the reaction tank is an active tank body, and has a wheel that can be moved to a tunnel type sterilization kettle. . The method for solid-state fermentation according to the full-function high-efficiency edible and medicinal solid-state fermentation system described in claim 1, wherein the reaction tank is a fixed tank body, which is a pressure vessel formed by a reinforced structure, and can be ventilated Sterilize directly in situ. The method for solid-state fermentation according to the full-function high-efficiency edible and medicinal solid-state fermentation system described in claim 1, wherein the spray lines are detachably connected to the liquid line to sprinkle head or sprayer The method is to carry out the bacteria carrier, or it can be used as an action of adding a liquid feed. The method for solid-state fermentation according to the full-function high-efficiency edible and medicinal solid-state fermentation system described in claim 1, wherein the full-function solid-state fermentation device further comprises a drying cycle unit, which is included in the front tank cover of the reaction tank The opposite tank body is respectively provided with a hot dry gas inlet and a hot dry gas outlet, and the front tank cover and the opposite tank body are provided with a plurality of hot gas dispersing ports, and each hot gas dispersing port corresponds to at least one culture disc laying layer. To provide uniform hot gas dispersion to each of the culture tray placement layers. The method for solid-state fermentation according to the full-function high-efficiency edible and medicinal solid-state fermentation system described in claim 6 wherein when the reaction tank is a pressure vessel, the outlet of the hot dry gas can be connected to a vacuum exhaust pipe. , vacuum drying. The method for solid-state fermentation according to the full-function high-efficiency medicinal medicinal solid-state fermentation system described in claim 6 of the patent application, wherein the drying cycle unit replaces the gas internal pipeline as in claim 1 of the patent application. The method for solid-state fermentation according to the full-function high-efficiency edible and medicinal solid-state fermentation system described in claim 1, wherein the product recovery unit further comprises a harvesting bucket and is placed on the movable shelf The culture tray can be pulled out from the front surface of the culture tray, and the dried medium can be dropped onto the collection tray and collected into the collection barrel.