TW201440804A - Non-alcohol mouthwash composition without chemical antibacterial medicament - Google Patents

Abstract

The present invention discloses a non-alcohol mouthwash composition without chemical antibacterial medicament. Based on the total weight of the non-alcohol mouthwash composition, the non-alcohol mouthwash composition comprises: from 0.03% to 1.5% by weight of γ -poly glutamic acid (γ -PGA) and from 0.5% to 4% by weight of surfactant. The non-alcohol mouthwash composition preferably comprises from 0.4% to 1.5% by weight of γ -PGA, especially preferably from 0.8% to 1.3% by weight of γ -PGA. With the implementation of the present invention, the non-alcohol mouthwash composition without chemical antibacterial medicament can effectively sterilize the oral cavity without stimulates the oral mucosa.

Description

Mouthwash composition that is non-alcoholic and has no chemical antibacterial agents

The present invention is a mouthwash composition, particularly a mouthwash composition that is non-alcoholic and has no chemical antimicrobial agents.

The mouth that comes into contact with food every day is a hotbed of bacteria, and bacteria in the mouth are prone to common oral diseases such as tooth decay and periodontal disease. There are more than 600 kinds of bacteria in the mouth. The common ones are Capnocytophaga sputigena, Lactobacillus brevis, Staphylococcus aureus, Streptococcus mutans, and oral chain. Staphylococcus oralis, Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus thermophilus. However, the toothbrush can only clean 50% of the tooth surface, while other parts require the assistance of floss and mouthwash.

The conventional mouthwash component contains synthetic chemical constituents (such as chlorhexidine gluconate CHX or cetylpyridinium chloride) and alcohol. These mouthwash components can cause irritation to the oral mucosa of ordinary people and cause teeth to become Yellow, affecting the appearance. Not to mention the treatment of radiotherapy or radiation therapy for the head and neck. Because the oral mucosa is inflamed due to the course of treatment, it is more dependent on mouthwash to maintain the hygiene of the oral environment. However, the oral mucosa which is more vulnerable due to chemotherapy or radiation therapy is a mouthwash that cannot withstand such stimulation.

In addition, in addition to alcohol, it is easy to cause white lesions in the oral mucosa. Will affect the concentration of wine measurement, and is not suitable for oral disinfection after dinner. To this end, there is a need for a mouthwash composition that does not irritate the oral mucosa and achieves good oral bacteriostatic effects, and is suitable as a mouthwash for oral disinfection after a meal.

The present invention is a mouthwash composition for non-alcoholic and non-chemical antibacterial agents comprising: gamma-polyglutamic acid and an interfacial active agent. Wherein the gamma-poly glutamic acid comprises between 0.03% and 1.5%, more preferably between 0.4% and 1.5%, more particularly between 0.8% and 1.3%, based on the total weight of the mouthwash composition. The invention can achieve the effect of simultaneously protecting the oral mucosa and effectively inhibiting bacteria.

The present invention provides a mouthwash composition non-alcoholic and non-chemical antibacterial agent comprising: γ-poly glutamic acid, which is between 0.03% and 1.5% by weight of the mouthwash composition; and an interface The active agent, which comprises between 0.5% and 4% by weight of the total mouthwash composition.

Through the implementation of the present invention, at least the following advancements can be achieved: first, effective oral bacteriostatic effect can be achieved; second, damage to the oral mucosa can be avoided; and third, the wine measurement result can be avoided after use.

In order to make the technical content of the present invention known to those skilled in the art and implemented accordingly, and according to the contents disclosed in the present specification, the scope and drawings of the patent application The detailed features and advantages of the present invention are described in detail in the embodiments of the invention.

1A is a diagram showing the antibacterial activity of an Escherichia coli reacted with different concentrations of γ-poly glutamate mouthwash composition by an optical density method according to an embodiment of the present invention; FIG. 1B is a view of a large intestine according to an embodiment of the present invention; The bacteriostatic activity map of the bacillus reacting with different concentrations of γ-poly glutamate mouthwash composition by the pan method; FIG. 2A is a S. aureus with different concentrations of γ-poly gluten according to an embodiment of the invention The amino acid mouthwash composition reacts with the bacteriostatic activity spectrum measured by the optical density method; FIG. 2B shows a reaction of the Staphylococcus aureus with different concentrations of the γ-poly glutamate mouthwash composition for coating according to an embodiment of the present invention a bacteriostatic activity diagram of the disk method; FIG. 3A is a diagram showing the bacteriostatic activity of a Pseudomonas aeruginosa reacting with different concentrations of γ-poly glutamate mouthwash composition by an optical density method according to an embodiment of the present invention; And FIG. 3B is a graph showing the antibacterial activity of a Pseudomonas aeruginosa reacting with different concentrations of γ-poly glutamate mouthwash composition by a coating method according to an embodiment of the present invention.

An embodiment of the present invention is a mouthwash composition that is non-alcoholic and has no chemical antibacterial agent, and includes: γ-poly glutamic acid and an interfacial active agent.

Γ-poly glutamic acid (γ-PGA) was first discovered in the cell wall of Bacillus anthracis in 1973. It is a novel fermentation by microorganisms (such as Bacillus subtilis). Natural polymer It has many excellent properties: non-toxic, high biocompatibility, biodegradability, antibacterial activity, water retention and adsorption, and is inexpensive.

Due to the above-mentioned excellent properties, γ-poly glutamic acid can be used as a tackifier and stabilizer in the food industry; a heavy metal adsorbent for water treatment; a moisturizer for the chemical industry; a soil conditioner for agriculture; and a health and food industry. Nutritional supplements. In particular, the inhibition of microbial and food safety properties makes γ-poly glutamic acid an emerging material in the food, cosmetic and biomedical materials industries. Therefore, γ-poly glutamic acid is particularly used as a bacteriostatic agent in the composition of the mouthwash in the present embodiment.

Accordingly, the mouthwash composition comprises gamma-polyglutamic acid in an amount between 0.03% and 1.5% by weight based on the total weight of the mouthwash composition, in place of the irritating chemical composition commonly used in commercially available mouthwash compositions, Thereby improving the cleanliness and health of the mouth. Wherein, the γ-poly glutamic acid may preferably be between 0.4% and 1.5% by weight of the mouthwash composition, and more particularly between 0.8% and 1.3% of the total weight of the mouthwash composition. . The higher the proportion of γ-poly glutamic acid, the more bacteriostatic effect, but if the ratio is too high, the viscosity of the mouthwash will be too high, which affects the taste of the mouth.

The surfactant, which is between 0.5% and 4% by weight of the mouthwash composition. The surfactant may be such that the lipophilic component and the hydrophilic component of the mouthwash composition are blended together to form a uniform mouthwash composition, preferably from 2% by weight based on the total weight of the mouthwash composition. Between 3%. Wherein, the surfactant may be glycerin, and the concentration of glycerin may range from 95% to 99% by weight, more preferably 95% by weight.

The mouthwash composition of the non-alcoholic and non-chemical antibacterial agent may further comprise potassium nitrate, which is between 0.001% and 3%, based on the total weight of the mouthwash composition. Potassium nitrate can release potassium ions, and free potassium ions can block nerves. It reduces the pain through conduction, which in turn can relieve the symptoms of tooth sensitivity for a short time.

The mouthwash composition of the non-alcoholic and non-chemical antibacterial agent may further comprise Chlorhexidine Gluconate, which is between 0.001% and 0.1% by weight of the total mouthwash composition. Chlorhexidine gluconate is a commonly used bacteriostatic agent, but excessive addition tends to irritate the oral mucosa. Γ-poly glutamic acid with bacteriostatic effect has been included in the mouthwash composition of non-alcoholic and non-chemical antibacterial agents, so only a very small amount of chlorhexidine gluconate is added to enhance the bacteriostatic effect, and a very small amount Chlorhexidine gluconate does not cause mucosal irritation.

The mouthwash composition of the non-alcoholic and non-chemical antibacterial agent may further comprise a perfume in the range of from 0.05% to 0.3% by weight based on the total weight of the mouthwash composition. The fragrance may be a spearmint essential oil, a peppermint essential oil or a combination of the two. The role of the fragrance is to enhance the aroma or mouthfeel of the mouthwash composition which is non-alcoholic and free of chemical antibacterial agents. For example, spearmint essential oil can enhance the aroma of non-alcoholic and non-chemical antiseptic mouthwash compositions, while peppermint essential oils can make the mouthwash composition of non-alcoholic and non-chemical antiseptic agents fresh. Peppermint essential oil may range from 0.1% to 0.2% by weight of the mouthwash composition.

A mouthwash composition that is non-alcoholic and has no chemical antibacterial agent may further include a food coloring such as bright blue pigment. Since it is difficult for the user to directly distinguish between normal water and mouthwash by using the mouthwash, it is easy to recognize the mouthwash composition which is non-alcoholic and has no chemical antibacterial agent by adding the food coloring. Generally, the concentration of bright blue pigment can be 2.5 mg/L.

In order to verify that the above-mentioned mouthwash composition containing non-alcoholic γ-polyglutamic acid and no chemical antibacterial agent has an effective bacteriostatic ability. Use of Escherichia coli (ATCC 23815), Staphylococcus aureus (ATCC 10832) and Pseudomonas aeruginosa (ATCC) The standard strain of 10145) was subjected to a bacteriostatic test. It can be seen from the growth curves of the three standard strains that after about eight hours, the three standard strains can reach the logarithmic growth phase, so the reaction time is set to eight hours.

Next, the following five non-alcoholic and non-chemical antibacterial mouthwash compositions are configured, and each of the non-alcoholic and non-chemical antibacterial mouthwash compositions comprises different weight percentages of gamma-polyglutamic acid: 1) Mix 100 mg of γ-poly glutamic acid powder, 744 μl of spearmint, 20 ml of glycerin (99.5%) and 2.5 mg of bright blue pigment (CI.42090) in one liter of water and stir. To uniformity, γ-poly glutamic acid accounts for 0.01% of the total weight of the mouthwash composition which is non-alcoholic and has no chemical antibacterial agent; (2) 500 mg of γ-poly glutamic acid powder is mixed in one liter of water, 744 μl of spearmint, 20 ml of glycerin (99.5%) and 2.5 mg of bright blue pigment (CI.42090), and stirred until homogeneous. γ-polyglycolic acid is non-alcoholic and has no chemical antibacterial agents. The total weight of the mouthwash composition is 0.05%; (3) 1000 mg of γ-poly glutamic acid powder, 744 μl of spearmint, 20 ml of glycerin (concentration 99.5%) and 2.5 mg in one liter of water Bright blue pigment (CI.42090), and stirred until uniform, γ-poly glutamic acid accounts for non-alcoholic and no chemical antibacterial The total weight of the mouthwash composition is 0.1%; (4) 5000 mg of γ-poly glutamic acid powder, 744 μl of spearmint, 20 ml of glycerin (concentration 99.5%) and 2.5 are mixed in one liter of water. mM bright blue pigment (CI.42090) and stirred until homogeneous, γ-poly glutamic acid accounts for 0.5% of the total weight of the non-alcoholic and non-chemical antibacterial mouthwash composition; and (5) in one liter Mix 10,000 mg of γ-poly glutamic acid powder, 744 μl of spearmint, 20 ml of glycerin (99.5% by weight) and 2.5 mg of bright blue pigment (CI.42090), and stirred until homogeneous, γ-poly glutamic acid accounts for 1% of the total weight of the mouthwash composition which is non-alcoholic and has no chemical antibacterial agent.

The above five kinds of non-alcoholic and non-chemical antibacterial mouthwash compositions (experiment group 1~5), commercial product A (experiment group 6), and commercial product B (experiment group 7) were placed in an aseptic workstation. Commercially available product C (experimental group 8) also used 9 ml of physiological saline as a control group, and was separately added to a sterile glass test tube. Next, 1 mL of Escherichia coli bacterial solution (photometric value (OD) of 0.1 and colony number of 10 ⁶ CFU/ml) was further added to each sterile glass test tube and mixed for optical density measurement. At the same time, the bacterial liquid was taken out from each of the sterile glass tubes and the diluted bacterial solution was applied to a solid medium for coating measurement. Then, each sterile glass test tube and each solid medium were cultured in a 37 ° C incubator for 8 hours, and the absorbance after each sterile glass test tube culture and the number of colonies after each plate culture were determined.

The antibacterial activity of the densitometry method is the difference between the absorbance after incubation in the control group and the absorbance after incubation in the experimental group divided by the absorbance after incubation in the control group. The antibacterial activity of the coating method was the difference between the number of colonies after the culture of the control plate and the number of colonies after the plate culture in the experimental group, and the number of colonies after the plate culture in the control group, and the data of the antibacterial activity were drawn. Into the chart. Next, after the completion of E. coli, the above experiments were repeated with Staphylococcus aureus and Pseudomonas aeruginosa, respectively, to obtain bacteriostatic activity data of Staphylococcus aureus and Pseudomonas aeruginosa and plotted.

As shown in Fig. 1A and Fig. 1B, for E. coli, it can be seen by both the densitometry method and the plate coating method: five kinds of non-alcoholic and non-chemical antibacterial agent mouthwash compositions (experiment group 1) In the comparison of ~5), the mouthwash compositions of the experimental group 4 and the experimental group 5 had bacteriostatic activity of up to 90% or more, which included 0.5% by weight and 1% by weight of γ-poly glutamic acid, respectively. Because experimental groups 4 to 5 have better The bacteriostatic activity was further compared between experimental group 4 and experimental group 5 with other commercially available mouthwash compositions.

Table 1 is a table showing the antibacterial activity of an Escherichia coli and a mouthwash composition of different experimental groups measured by an optical density method according to an embodiment of the present invention. Table 2 is a table showing the antibacterial activity of E. coli and the mouthwash composition of different experimental groups by the coating method. As shown in the following Tables 1 and 2, the mouthwash composition of the experimental group 4 and the experimental group 5 is comparable to the commercially available product A, the commercially available product B, and the commercially available product C, and has an antibacterial activity against Escherichia coli. Mouthwash compositions containing irritating chemicals are available in over 95%.

As shown in Figures 2A and 2B, for Staphylococcus aureus, it can be seen by both the densitometry method and the coating method: five kinds of non-alcoholic and non-chemical antibacterial mouthwash compositions (experiment In the comparison of groups 1 to 5), the mouthwash compositions of the experimental group 2, the experimental group 3, the experimental group 4, and the experimental group 5 have an antibacterial activity of more than 90%, which includes 0.05% by weight and 0.1% by weight, respectively. 0.5% by weight and 1% by weight of γ-polyglutamic acid.

Table 3 is a different form of Staphylococcus aureus according to an embodiment of the present invention The mouthwash composition of the experimental group was reacted by the optical density method to measure the bacteriostatic activity. Table 4 is a table showing the antibacterial activity of Staphylococcus aureus reacting with the mouthwash composition of different experimental groups by the coating method according to an embodiment of the present invention. As shown in the following Tables 3 and 4, the mouthwash compositions of the experimental group 4 and the experimental group 5 have comparable antibacterial activity against Staphylococcus aureus as compared with the commercially available product A, the commercially available product B, and the commercially available product C. Mouthwash compositions containing commercially available irritating chemicals are as high as 95% or more.

As shown in Fig. 3A and Fig. 3B, for Pseudomonas aeruginosa, it can be seen by both the densitometry method and the plate coating method: a mouthwash composition of five non-alcoholic and non-chemical antibacterial agents (experimental group) In the comparison of 1 to 5), the mouthwash composition of the experimental group 5 has an antibacterial activity of up to 90% or more, which includes 1% by weight of γ-poly glutamic acid.

Table 5 is a table showing the antibacterial activity of a Pseudomonas aeruginosa and a mouthwash composition of different experimental groups measured by an optical density method according to an embodiment of the present invention. Table 6 is a table showing the antibacterial activity of a Pseudomonas aeruginosa and a mouthwash composition of different experimental groups measured by a pan method. As shown in the following Tables 5 and 6, the mouthwash composition of the experimental group 5 has a bacteriostatic activity against Pseudomonas aeruginosa comparable to that of the commercially available product A, the commercially available product B, and the commercially available product C. Mouthwash composition of sex chemicals The objects are all over 90%.

It can be seen from the above that the mouthwash composition of the non-alcoholic and non-chemical antibacterial agent according to the embodiment of the present invention comprises γ-poly glutamic acid, which is between 0.03% and 1.5% by weight based on the total weight of the mouthwash composition, γ- A mouthwash composition having a lower ratio of total glutamic acid to the mouthwash composition (for example, 0.05% by weight of γ-poly glutamic acid) can only inhibit the growth of Staphylococcus aureus, and γ-poly glutamic acid accounts for 漱A mouthwash composition with a moderate proportion of the total weight of the saliva composition (for example, 0.5% by weight of γ-polyglutamic acid) can inhibit Staphylococcus aureus and Escherichia coli, and γ-poly glutamic acid accounts for the total weight ratio of the mouthwash composition. A high mouthwash composition (for example, 1% by weight of γ-polyglutamic acid) can simultaneously inhibit the growth of Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa, so γ-poly glutamic acid can be more particularly The saliva composition has a total weight of between 0.8% and 1.3% to replace the irritating chemical composition commonly used in commercially available mouthwash compositions and to maintain the same bacteriostatic efficacy as commercially available mouthwashes.

The embodiments are described to illustrate the features of the present invention, and the purpose of the present invention is to enable those skilled in the art to understand the present invention and to implement the present invention without limiting the scope of the present invention. Complete the spirit of revealing Equivalent modifications or modifications are still included in the scope of the patent application described below.

Claims (10)

A mouthwash composition non-alcoholic and non-chemical antibacterial agent, comprising: γ-poly glutamic acid, which is between 0.03% and 1.5% by weight of the total mouthwash composition; and an surfactant, It is between 0.5% and 4% of the total weight of the mouthwash composition. The mouthwash composition of claim 1, wherein the γ-poly glutamic acid comprises between 0.4% and 1.5% by total weight of the mouthwash composition. The mouthwash composition of claim 1, wherein the γ-poly glutamic acid comprises between 0.8% and 1.3% by total weight of the mouthwash composition. The mouthwash composition of claim 1, wherein the surfactant comprises between 2% and 3% by weight of the mouthwash composition. The mouthwash composition of claim 4, wherein the surfactant is glycerin, and the concentration of the glycerin ranges from 95% to 99% by weight. The mouthwash composition of claim 1, further comprising potassium nitrate, which is between 0.001% and 3% by total weight of the mouthwash composition. The mouthwash composition of claim 1, further comprising Chlorhexidine Gluconate, which is between 0.001% and 0.1% by weight of the total mouthwash composition. The mouthwash composition of claim 1, further comprising a fragrance comprising between 0.05% and 0.3% by weight of the total mouthwash composition. The mouthwash composition of claim 8, wherein the fragrance is spearmint essential oil, peppermint essential oil or a combination of the two. a mouthwash composition as described in claim 9, wherein the spearmint The oil accounts for between 0.1% and 0.2% of the total weight of the mouthwash composition.