TW201437363A - Biohomogenizer - Google Patents

Abstract

The present invention provides a biohomogenizer to cut a sample into small pieces. The biohomogenizer at least comprises a filter and a cutter disposed beside the filter. When the sample is put into the filter and partly passes through the filter, the portion of the sample passed through the filter is shaped and further cut by the cutter to be separated from the sample. According to the present invention, the size of the cut sample, such as toughness biological tissues with irregular shape, can be adjusted, and the prior disadvantages can be further avoided.

Description

Biomass device

The present invention relates to a bio-homogenization device, and more particularly to a bio-homogenization device that can adjust the particle size specification of the material to be cut and avoid repeated homogenization of the material to be cut.

Tissue culture is a technique for aseptically taking out tissue blocks and cell populations from individuals of multicellular organisms and continuing their living under appropriate conditions. Among them, there is a culture in vivo in which a taken tissue is transplanted and cultured in a certain part of another organism, and a culture in vitro cultured in a glass, but generally referred to as tissue culture after multi-finger By. Nowadays, tissue culture technology is widely used not only in the research of cell proliferation, differentiation, and cancer, but also in tissue culture cells for drug effects, and is also being used to manufacture vaccines. In addition, since a large and single cell population can be obtained by tissue culture, it is also quite convenient to use as a biochemical research material. Therefore, in the entire field of biological sciences, organizational training has become an indispensable technology.

Among them, cell culture is a form of generalized tissue culture, which may mean that an organ or a tissue is treated with trypsin or the like to separate the cells, and then transferred to a glass container for cultivation. As mentioned above, since higher organisms are composed of multiple cells, it is very difficult to study the functional activities of individual cells or a certain group of cells in vivo under the overall conditions, but if the living cells can be cultured in vitro, observe And research is much more convenient. Therefore, when performing cell culture of an organ or tissue, the first step is to perform preliminary cleavage separation of the organ or tissue. In the past, when cells or tissues were separated, surgical instruments (such as scalpels, scissors, and tweezers) were used to cut, cut, and tear cells or tissues.

However, living cells must survive and undergo physiological activities under certain physiological conditions, especially for higher animal and plant cells. The living conditions are extremely strict and slightly discomfort. The cell will die. Therefore, cell culture technology is the technology for cultivating and researching living cells using optimal survival conditions. That is to say, the above steps of cell or tissue separation must be performed in a sterile environment throughout the process, and must be completed by a highly experienced operator with a high degree of mental concentration for a long time. However, when the processing time of this step is too long, not only the subsequent cell survival rate is adversely affected, but also the operator's contamination rate of the sample is increased due to long-time repetitive operations.

Later, although the development of homogenizers has overcome the problem of operating time, the speed and time can be further adjusted to achieve a consistent effect on each isolated cell or tissue sample. However, since the original design concept of the homogenizer is used for tissue homogenization, and its subsequent purpose is to analyze DNA, RNA or protein in the tissue, it is not for cell culture purposes. Therefore, the homogenizer is quite powerful against organs or tissues, and it is not advantageous for the isolation and culture of subsequent cells.

In addition, the homogenizer lacks a sorting mechanism, and the cut cells or tissue samples remain in the homogenizer, and then the cutting and whipping action is repeated, which is also disadvantageous for subsequent cells or tissues. Training process.

In view of the above, the present invention provides a bio-homogenization device for performing the same cutting, the device comprising at least one sifting unit and a cutting unit adjacent to one of the sifting units. Wherein, when the sample is placed in the sifting unit and partially passed through the sifting unit, the portion of the sample that passes through the sifting unit is shaped and cut by the cutting unit to be separated from the sample.

In an embodiment of the invention, the sifting unit has at least one hole, and the sample passes through the sifting unit through the hole portion, and the portion of the sample that passes through the sifting unit is cut by the cutting unit and is a granule. Structure. Preferably, the hole of the sifting unit is circular and has a predetermined size, and the granular structure is of a predetermined size.

In an embodiment of the invention, the biological homogenization device further comprises a rotating shaft and a pressing rod connected to the rotating shaft. Wherein, the rotating shaft and the pressing rod system and the cutting unit are respectively disposed on opposite sides of the sieve leakage unit, and the sample system is placed between the pressing rod and the sieve leakage unit, and when the rotating shaft rotates and drives the pressing rod to apply an external force At the time of the sample, the sample portion is passed through the sifting unit. Preferably, the bio-homogenization device further comprises a driving member. Wherein, the driving member is connected to the rotating shaft to drive the rotating shaft The movement, in turn, drives the pressure bar to apply an external force to cause the sample portion to pass through the sieve leakage unit. Preferably, the driving member comprises a rocker or a motor.

In an embodiment of the invention, the biological homogenization device further comprises a cover body and a receiving unit that can be closely fitted to the cover body. Wherein, the rotating shaft and the pressing rod are located in the cover body, and the sieve leakage unit and the cutting unit are detachably mounted in the accommodating unit, and when the sample passes through the sifting unit and is cut by the cutting unit and separated from the sample, Dropped into the accommodating unit.

In an embodiment of the invention, the rotating shaft, the pressing rod, the sifting unit and the cutting unit respectively have through-holes, and the intermediate through-holes communicate with each other to allow a liquid to enter through the intermediate through-holes Inside the unit. Preferably, the biological homogenization device further comprises at least one sealing unit, wherein the sealing unit is disposed on one side of the through hole in the rotating shaft to seal the middle through hole.

In an embodiment of the invention, the cutting unit comprises a cutting piece.

In an embodiment of the invention, wherein the sample is from a biological tissue. Preferably, the biological tissue may be a brain tissue, a liver tissue, a lung tissue, a muscle tissue or a fat tissue.

The features of the present invention and its advantages will be further understood from the following description. For reference, please refer to the first to fifth figures.

100‧‧‧Biohomogeneous device

1‧‧‧Draining unit

11‧‧‧Circular holes

2‧‧‧Cutting unit

21‧‧‧ cutting piece

3‧‧‧Rotary axis

4‧‧‧Press

5‧‧‧ Drives

6‧‧‧ Sealing unit

61‧‧‧Waterproof tablets

62‧‧‧Water Block

7‧‧‧ Cover

71‧‧‧First screwing

8‧‧‧ housing unit

81‧‧‧Second screw joint

F‧‧‧External force

S1‧‧‧ groove

S2‧‧‧ accommodating space

H‧‧‧through hole

R‧‧‧Aperture of circular holes

T‧‧‧ sample

T‧‧‧granular structure

1 is a cross-sectional view showing the structure of a bio-homogenizing device according to an embodiment of the present invention; FIG. 2 is a view showing an exploded view of a structure of a bio-homogenizing device according to an embodiment of the present invention; and FIG. 3 is a view showing a drier unit according to an embodiment of the present invention; 4 is a schematic view showing the appearance of a biological homogenizing device according to an embodiment of the present invention; and a fifth drawing showing a part of the sample passing through the sifting unit and being cut by the cutting unit, and then dropped to the capacity. Schematic diagram of the operation stored in the unit.

Please refer to the first figure and the second figure. The first figure shows a schematic cross-sectional view of the structure of the biological homogenizing device according to an embodiment of the present invention, and the second figure shows the biological homogenizing device according to an embodiment of the present invention. Explosion diagram of the component of the structure. Among other things, the present invention provides a biological homogenization apparatus 100 comprising at least one sifting unit 1 and a cutting unit 2. As shown, the cutting unit 2 is disposed adjacent to the sifting unit 1, so that when a sample (not shown) enters the sifting unit 1 and a portion thereof is squeezed out of the sifting unit 1, these are extruded. A part of the sample is shaped and then cut by the cutting unit 2 disposed beside the sifting unit 1 to be separated from the original sample.

Please refer to the third figure. The third figure shows a top view of the sifter unit in an embodiment of the present invention. As shown, in the preferred embodiment, the sifter unit 1 is a sleeve having a central through hole H and has a recess S1 for receiving a sample. The bottom of the groove S1 includes a plurality of circular holes 11 . When the sample passes through the sieve unit 1 through the circular hole, the portion of the sample that passes through the sieve unit 1 is cut by the cutting unit 2 to become a particle. Structure. Further, the circular hole 11 of the sifter unit 1 has a predetermined size, so that the granular structure finally formed by cutting by the cutting unit 2 will be a granular structure having the above-described predetermined size. Furthermore, since the predetermined size of the circular hole 11 of the sifting unit 1 is different depending on the particle size of the organ to be cut, the present invention can be applied to a soft and irregular biological tissue, for example: Brain tissue, liver tissue, lung tissue, muscle tissue or adipose tissue.

Preferably, the sifter unit 1 has a structure having a circular multi-aperture of 3 mm, but the invention is not intended to be limited thereto. In addition, it should be noted that the circular hole is only described in a preferred embodiment, that is, the sifting unit 1 used in the present invention may also have other shapes of holes, depending on the biological tissue to be used. It is not intended to be limited to any embodiment.

As for the cutting unit 2, it may preferably be a sleeve which also has a middle through hole H, and the bottom portion thereof comprises a cutting piece 21. In a preferred embodiment, the cutting blade 21 is a sheet of plastic material having a cutting function. Next, as shown, the sifter unit 1 can be embedded in the cutting unit 2 such that the outer wall of the sifter unit 1 fits against the inner wall of the cutting unit 2, and the cutting piece 21 of the cutting unit 2 is located just in the circular hole. exit.

Next, referring to the first and second figures, the biological homogenization device 100 further includes a rotating shaft 3 and a pressing rod 4 connected to the rotating shaft 3. The rotating shaft 3 and the pressing rod 4 and the cutting unit 2 are respectively disposed on opposite sides of the sifting unit 1. Preferably, the rotating shaft 3 and the pressing rod 4 also have a middle through hole, and the inner wall of the through hole in the pressing rod 4 and the outer wall of the through hole in the cutting unit 2 are correspondingly provided with threads. Therefore, the pressing rod 4 can be further set on the cutting sheet In combination with the element 2, when the sample is placed in the groove S1 of the sifter unit 1, the rotating shaft 3 can be rotated to drive the pressure rod 4 along the thread of the outer wall of the through hole in the cutting unit 2 toward the screen drain. Unit 1 is approached and an external force is applied to the sample. At this time, when the sample is pressed by the pressing rod 4 and partially passes through the circular hole and exits the sifting unit 1, the cutting piece 21 at the exit of the circular hole can cut the extruded portion. It is separated from the original sample. However, the manner of connecting the rotating shaft 3 and the pressing rod 4, and the connecting manner of the pressing rod 4 and the cutting unit 2 are all described in an embodiment, and the present invention is not limited thereto.

In the above, the biological homogenization device 100 further includes a driving member 5. The driving member 5 is connected to the rotating shaft 3 to drive the rotating shaft 3 to rotate, thereby driving the pressing rod 4 to apply an external force to pass the sample portion out of the sifting unit 1. Preferably, the driving member 5 can be a rocker (as shown in the first and second figures) or a motor.

Furthermore, the biological homogenization device 100 further includes a cover 7 and a receiving unit 8. As shown in the first and fourth figures, the cover 7 and the accommodating unit 8 can be closely fitted to form an accommodating space S2. For example, the inner side of the cover body 7 may have a first screwing portion 71, and the outer edge of the upper opening of the accommodating unit 8 may be correspondingly provided with a second screwing portion 81, so the cover body 7 and the accommodating unit 8 is formed by the cooperation of the first screwing portion 71 and the second screwing portion 81 to form a sealed accommodating space S2. At this time, as shown in the first figure, the rotating shaft 3 and the pressing rod 4 are located in the cover body 7, and the driving member 5 (taking the rocker as an example) is detachably sleeved on the cover body 7, in the meantime. The combination may be a snap-fit or an integrally formed component from the beginning, and the invention is not intended to be limited thereto. The sifting unit 1 and the cutting unit 2 are detachably mounted on the accommodating unit 8, and preferably close to the upper opening of the accommodating unit 8, and the erecting manner can be a snapping or other kind of disassembly at any time. In the fixed manner, the present invention is not intended to be limited thereto. Therefore, when the portion of the sample that has passed through the sifting unit 1 is cut by the cutting unit 2 and separated from the sample, it is dropped into the accommodating space S2 of the accommodating unit 8.

As described above, the rotating shaft 3, the pressing rod 4, the sifting unit 1 and the cutting unit 2 have through-holes H, respectively, and the intermediate through-holes H communicate with each other. Therefore, the bio-homogenization device 100 further includes at least one sealing unit 6, and the material of the sealing unit 6 is preferably a recoverable silicone material. As shown in the first figure, since the middle through hole outlet of the cutting unit 2 communicates with the accommodating space S2, one of the two sides of the middle through hole of the rotating shaft communicates with the middle through hole of the pressing rod, so that the sealing The unit 6 is disposed on the other side of the through hole in the rotating shaft to place the entire bio-homogenizing device 100 in a sealed state after being assembled. In the preferred embodiment, the driving member 5 can also have a middle through hole. At this time, the sealing unit 6 is interposed between the through hole in the driving member 5 and the through hole in the rotating shaft 6. In addition, the sealing unit 6 includes a waterproof sheet 61 and a water blocking sheet 62, and the liquid is injected into the accommodating space S2 after being punctured by an object such as a needle or a straw.

The composition and combination of the units of the bio-homogenizing device 100 provided by the present invention have been described in detail as before, and the operation mode of the above device will be further described by the fifth figure. First, please refer to the fifth figure, and with the first figure, the second figure and the fourth figure, when the organ tissue (ie, sample T) is cut, the collecting unit 8 for collection is erected, and the cutting unit is sequentially arranged. 2 and the sifting unit 1, the organ tissue T is placed in the groove S1 of the sifting unit 1, and then the lid 7 to which the rotating shaft 3, the pressing rod 4 and the sealing unit 6 have been integrated is screwed. Finally, the driving member 5 is fitted over the cover 7, and the driving member 5 is rotated in the clockwise direction. At this time, the driving member 5 drives the rotating shaft 3 to push the pressing rod 4, and the pressing rod 4 is pressed toward the sifting unit 1, thereby applying an external force F to the organ tissue T below it.

Then, under appropriate pressure, part of the organ tissue will pass through the sifting unit through the circular hole 11 having the predetermined size R. At this time, the organ tissue that has passed out has been shaped, and then the cutting piece of the lower cutting unit 2 is passed. The cut and cut granular tissue t will fall into the accommodating unit 8 and be collected and stored according to the natural gravity method.

Finally, when the cutting is completed to remove the driving member 1 (if the driving member also has a middle through hole, it may not be removed), the enzyme solution or the buffer solution may be sucked by the needle or the straw, and the container is punctured by the upper sealing unit 6 and injected into the container. The accommodating space S2 of the unit 8 is mixed with the diced tissue t for subsequent reaction, such as cell culture or the like.

In summary, the bio-homogenization device provided by the present invention can adjust the particle size specification of the cut object, that is, the soft and tough texture can be shaped through the circular multi-aperture sieve-drain unit. Further cutting to obtain a granular structure will effectively adjust the fineness of the tissue, overcoming the problem that the homogenizing device can only whipped the structure of powder, mud, juice and the like.

Furthermore, since the cutting unit 2 is disposed below the sifting unit, the cut tissue will fall into the accommodating unit according to the natural gravity method, and the tissue can be prevented from being repeatedly crushed and whipped. Cell death, which solves the problem of repetitive homogenization and damage to cells The problem of harm.

The detailed description of the preferred embodiments of the present invention is intended to be limited to the scope of the invention, and is not intended to limit the scope of the invention. Within the scope of the patent of the present invention.

100‧‧‧Biohomogeneous device

1‧‧‧Draining unit

2‧‧‧Cutting unit

21‧‧‧ cutting piece

3‧‧‧Rotary axis

4‧‧‧Press

5‧‧‧ Drives

6‧‧‧ Sealing unit

61‧‧‧Waterproof tablets

62‧‧‧Water Block

7‧‧‧ Cover

71‧‧‧First screwing

8‧‧‧ housing unit

81‧‧‧Second screw joint

S1‧‧‧ groove

S2‧‧‧ accommodating space

H‧‧‧through hole

Claims (12)

A biological homogenizing device for performing the same cutting, the device comprising at least: a sifting unit; and a cutting unit adjacent to the sifting unit, wherein when the sample is placed in the sifting unit, When a portion thereof is passed out of the sifting unit, a portion of the sample that passes through the sifting unit is shaped and cut by the cutting unit to be separated from the sample. The biological homogenizing device according to claim 1, wherein the sifting unit has at least one hole through which the sample passes through the sifting unit, and the sample passes through the sifting unit. Part of the cutting unit is cut into a granular structure. The biological homogenizing device according to claim 2, wherein the hole of the sifting unit is circular and has a predetermined size, and the granular structure has the predetermined size. The biological homogenizing device according to claim 1, further comprising a rotating shaft and a pressing rod connected to the rotating shaft, wherein the rotating shaft and the pressing rod and the cutting unit are respectively disposed on the screen leakage unit Opposite the two sides, and the sample is placed between the pressing rod and the sifting unit. When the rotating shaft rotates and drives the pressing rod to apply an external force to the sample, the sample portion passes through the sieve. Leak unit. The biological homogenizing device according to claim 4, further comprising a cover body and a receiving unit that can be tightly fitted to the cover body, wherein the rotating shaft and the pressing rod are located in the cover body, and the The sifting unit and the cutting unit are detachably mounted in the accommodating unit, and when the portion of the sample that passes through the sifting unit is cut by the cutting unit and separated from the sample, the detached unit is dropped into the accommodating unit. Inside. The biological homogenizing device according to claim 5, wherein the rotating shaft, the pressing rod, the sifting unit and the cutting unit respectively have a through hole therein, and the middle through holes communicate with each other to enable A liquid can enter the accommodating unit via the middle through holes. The biological homogenizing device according to claim 6, further comprising at least one sealing unit, wherein the sealing unit is disposed at one side of the middle through hole of the rotating shaft to seal the middle through hole. The bio-homogenizing device of claim 4, further comprising a driving member, wherein the driving member is coupled to the rotating shaft to drive the rotating shaft to rotate, thereby driving the pressing rod to apply the external force to the sample portion The sieve unit is worn out. The biological homogenizing device of claim 8, wherein the driving member comprises a rocker or a motor. The biological homogenizing device of claim 1, wherein the cutting unit comprises a cutting piece. The biological homogenization device of claim 1, wherein the sample is from a biological tissue. The biological homogenization device according to claim 11, wherein the biological tissue can be a brain tissue, a liver tissue, a lung tissue, a muscle tissue or a fat tissue.