TWM509807U - Device for preparing stem cell - Google Patents

Description

Stem cell preparation preparation device

The present invention relates to a device for preparing a stem cell preparation, in particular to a device for preparing a stem cell preparation which can adjust the particle size specification of the object to be cut and avoids repeated homogenization of the object to be cut, and the device particularly designs the driving element to be pressed to achieve a single Handy convenience.

Stem Cell is a non-differentiated primary cell in an organism that can be continuously replicated and renewed for a long time, and has the ability to differentiate into mature cells derived from specific types and functions. In general, the source of human stem cells can be mainly divided into embryonic stem cells and tissue stem cells. Embryonic stem cell lines are obtained from embryos remaining after infertility treatment, embryonic germ cells that are terminated by pregnancy, or obtained by cell fusion; The stem cells are obtained from organs or tissues of mature individuals, and the separated cells are treated by trypsin or the like.

In the cell culture of an organ or tissue, the first step is to initially cut and separate the organ or tissue. This step is traditionally carried out using surgical instruments (eg, scalpels, scissors, forceps). Or tissue cutting, cutting and tearing. However, after living cells are in vitro, they need to survive and perform physiological activities under certain physiological conditions. If there is slight discomfort, the cells will die. Therefore, the above steps of cell or tissue separation must be performed in a sterile environment throughout the process, and must be performed by an experienced operator for a long period of time and a high concentration of mental energy. However, when the processing time of this step is too long, not only the subsequent cell survival rate In addition to adverse effects, the operator's contamination rate of the sample will be increased due to long-term repetitive operations.

Later, although the development of homogenizers overcame the problem of operation time, the original design concept was used for tissue homogenization, and its subsequent purpose was to analyze DNA, RNA or protein in tissues, not for cell culture purposes. Therefore, such a homogenizer is quite destructive to an organ or tissue, and lacks a sorting mechanism, so that the cut cell or tissue sample remains in the homogenizer, and the cutting and whipping action is repeated. It is more unfavorable for the isolation and culture of subsequent cells or tissues.

In order to overcome the above problems, the prior art Taiwan patent case TW I477598 uses a rotary extrusion method to extrude an object and then cut it with a rotating cutting piece, and the cut object falls into the collecting tube. However, the disadvantages of this rotary extrusion method include: 1.) requiring two hands to operate; 2.) the rotational force and the rotational speed may vary depending on the operation of different operators or the same operator at different times; 3.) when rotating Air is generated, which increases the contact of the cut sample with the air and increases the bacterial infection of the cut sample.

In view of this, the present invention provides a stem cell preparation device for performing the same cutting, the device comprising: a driving component, the driving component comprising a rotating sleeve, a pressing seat and a spring, the rotating sleeve and the pressing a sleeve is disposed, and the spring is located between the pressing seat and the rotating sleeve, and the outer peripheral wall of the rotating sleeve is provided with at least one spiral guiding groove, and the bottom end of the spiral guiding groove is connected with an upward reset guiding groove, and And the rotating guide shaft is connected to the adjacent spiral guide groove; Unit, the screening unit has a sample and One of the pressing rods has a groove, the bottom of the groove has at least one hole; a cutting unit, the cutting unit is adjacent to the screen leakage unit; and a receiving unit, the screen leakage unit is coupled with the cutting unit The grounding device is disposed in the accommodating unit; and a cover body is closely fitted to the accommodating unit and has a hook on one side thereof.

In an embodiment of the present invention, the sample is placed between the pressing rod and the sifting unit, and when the driving element drives the rotating shaft to rotate and drives the pressing rod to apply an external force to the sample, The hole through which the sample portion passes through the sifting unit is cut by the cutting unit and separated from the sample, and then dropped into the accommodating unit.

In an embodiment of the present invention, the sample piercing portion is cut into a granular structure after being cut by the cutting unit. Preferably, the hole of the sifting unit is circular and has a predetermined size, and the granular structure has the predetermined size.

In an embodiment of the present invention, the rotating shaft and the pressing rod are located in the cover body.

In an embodiment of the present invention, the rotating shaft, the pressing rod, the sifting unit and the cutting unit respectively have a through hole therein, and the middle through holes communicate with each other to enable a liquid to pass through Some of the through holes enter the accommodating unit.

In an embodiment of the present invention, the stem cell preparation preparation device of the present invention further comprises at least one sealing unit, wherein the sealing unit is disposed at one side of the middle through hole of the rotating shaft to seal the middle through hole.

In an embodiment of the present invention, the cutting unit comprises a cutting piece.

In one embodiment of the present creation, the sample is from a biological tissue. Preferably, the biological tissue can be a brain tissue, a liver tissue, a lung tissue, a muscle tissue or A fat tissue.

The features and advantages of this creation can be further understood by the following description. Please refer to the first to fourth figures when reading.

100‧‧‧Stem cell preparation preparation device

1‧‧‧Draining unit

11‧‧‧ hole

2‧‧‧Cutting unit

21‧‧‧ cutting piece

3‧‧‧Rotary axis

31‧‧‧Upper joint

32‧‧‧ Lower joint

4‧‧‧Press

5‧‧‧Drive components

51‧‧‧Rotary sleeve

511‧‧‧Spiral guide

512‧‧‧Reset guide channel

513‧‧‧ recessed hole

52‧‧‧Press seat

521‧‧‧Medium hole

522‧‧‧Bumps

53‧‧‧ Spring

6‧‧‧ Sealing unit

61‧‧‧Waterproof tablets

62‧‧‧Water Block

7‧‧‧ Cover

8‧‧‧ housing unit

81‧‧‧Second screw joint

9‧‧‧hook

S1‧‧‧ groove

S2‧‧‧ accommodating space

F‧‧‧External force

H‧‧‧through hole

R‧‧‧Aperture of circular holes

T‧‧‧ sample

T‧‧‧granular structure

The first figure shows the exploded view of the components of the structure of the stem cell preparation preparation device in the embodiment of the present invention; the second figure shows the top view of the sieve leakage unit in the embodiment of the present creation; the third figure shows the preparation device for the stem cell preparation in the embodiment of the present creation; FIG. 4 is a schematic view showing the operation of a part of the sample in the embodiment of the present invention, which is cut out through the cutting unit and then dropped into the accommodating unit.

Please refer to the first figure, which shows an exploded view of the components of the structure of the preparation device for stem cell preparation in an embodiment of the present invention. The present invention provides a stem cell preparation preparation device 100 comprising at least a sieve drain unit 1 and a cutting unit 2. As shown in the figure, the cutting unit 2 is adjacent to the sifting unit 1, so that when a sample (not shown) enters the sifting unit 1, and part of it is squeezed by the reaction force of the sifting unit 1 rotating upward When the sifting unit 1 is discharged, these extruded partial samples are shaped, and are cut by the cutting unit 2 disposed beside the sifting unit 1 to be separated from the original sample.

Please refer to the second figure. The second figure shows a top view of the sifting unit in the embodiment of the present invention. As shown, in the preferred embodiment, the sifter unit 1 is a sleeve having a medium through hole H and has a groove S1 for receiving a sample. The bottom of the groove S1 includes a plurality of circular holes 11 . When the sample passes through the sieve unit 1 through the circular hole 11 , the portion of the sample that passes through the sieve unit 1 is cut by the cutting unit 2 and becomes a particle. Structure. In addition, the circular hole 11 of the sifter unit 1 has a pre- The size is set so that the granular structure finally formed by cutting by the cutting unit 2 will be a granular structure having the above-described predetermined size. Moreover, since the predetermined size of the circular hole 11 of the sifting unit 1 is different depending on the particle size of the organ to be cut, the present invention can be applied to a soft and irregular biological tissue, for example: Brain tissue, liver tissue, lung tissue, muscle tissue or adipose tissue.

Preferably, the sifter unit 1 is a structure having a circular multi-aperture of 3 mm, but the present invention is not intended to be limited thereto. In addition, it should be noted that the circular hole 11 is only described in a preferred embodiment, that is, the stencil unit 1 used in the present invention may have holes of other shapes, depending on the biological tissue to be applied. It is not intended to be limited to any embodiment.

As for the cutting unit 2, it is preferable that the cutting unit 2 also has a sleeve having the middle through hole H, and the bottom portion thereof includes the cutting piece 21. In a preferred embodiment, the cutting blade 21 is a sheet of plastic material having a cutting function. Next, as shown, the sifter unit 1 can be embedded in the cutting unit 2 such that the outer wall of the sifter unit 1 is attached to the inner wall of the cutting unit 2, and the cutting piece 21 of the cutting unit 2 is located just in the circular hole 11. exit.

Next, referring to the first figure, the stem cell preparation preparation device 100 of the present invention further includes a rotating shaft 3 and a pressing rod 4 having an upper joint 31 and a lower joint 32, and the lower joint 32 and the pressing rod 4 connection. The rotating shaft 3 and the pressing rod 4 and the cutting unit 2 are respectively disposed on opposite sides of the sifting unit 1. Preferably, the rotating shaft 3 and the pressing rod 4 also have a middle through hole H, and the inner wall of the through hole H in the pressing rod 4 and the outer wall of the through hole H in the cutting unit 2 are correspondingly provided with threads. Therefore, the pressing rod 4 can be further sleeved on the cutting unit 2 and combined with it. When the sample is placed in the groove S1 of the sifting unit 1, the rotating shaft 3 can be rotated to drive the pressing rod 4 along the cutting. The thread of the outer wall of the through hole H in the unit 2 approaches the screen leakage unit 1, and an external force is applied to the sample. At this time, when When the sample is pressed by the pressing rod 4 and partially passes through the circular hole 11 and exits the sifting unit 1, the cutting piece 21 located at the exit of the circular hole 11 can cut the extruded portion and make it The original sample was separated. However, the manner of connecting the rotating shaft 3 and the pressing rod 4, and the manner of connecting the pressing rod 4 and the cutting unit 2 are all described in an embodiment, and the present invention is not intended to be limited thereto.

In view of the above, the stem cell preparation preparation device 100 of the present invention further includes a driving member 5. The driving element 5 includes a rotating sleeve 51, a pressing seat 52, and a spring 53, wherein the rotating sleeve 51 is sleeved with the pressing seat 52, and the spring 53 is located between the rotating sleeve 51 and the pressing seat 52. The outer diameter of the rotating sleeve 51 is provided with a plurality of equally arranged spiral guiding grooves 511, and the bottom ends of the spiral guiding grooves 511 are connected to the upward straight reset guiding groove 512, and the reset guiding groove 512 is connected to the adjacent spiral. The guide groove 511 and the rotating sleeve 51 also have a recessed hole 513 for accommodating the spring 53. The pressing hole 52 of the pressing seat 52 can accommodate the spring 53 and the rotating sleeve 51, and is provided with a plurality of protrusions 522 for correspondingly being inserted into the spiral guiding groove 511 of the rotating sleeve 51, and the pressing seat 52 itself does not rotate, only Can be moved up and down. Further, the driving member 5 is coupled to the joint 31 above the rotating shaft 3 to drive the rotating shaft 3 to rotate.

At this time, the stem cell preparation preparation device 100 of the present invention can be held by one hand, and at the same time, the driving member 5 is driven by a finger: when the finger is applied to the pressing seat 52, the spring 53 is compressed, and the projection 522 and the spiral guide are used. When the groove 511 is engaged, the rotary sleeve 51 is driven to rotate when the pressure is pressed, and the rotating shaft 3 is rotated to apply an external force to the pressing rod 4 connected to the rotating shaft 3 to pass the sample portion out of the sifting unit 1. When the pressing seat 52 is pressed down to the bottom and the finger is released, the bump 522 enters the reset guiding groove 512, and then rebounds by the spring 53 to push up the pressing seat 52 to the original position for the next pressing action. , so repeated to achieve the effect of continuous rotation.

Furthermore, the stem cell preparation preparation device 100 of the present invention further includes a lid body 7 and a housing unit 8. As shown in the first and third figures, the cover 7 and the accommodating unit 8 can be tightly fitted to form A housing space S2. For example, the inner side of the cover body 7 may have a first screwing portion, and the outer edge of the upper opening of the accommodating unit 8 may be correspondingly provided with the second screwing portion 81, so the cover body 7 and the accommodating unit 8 are The sealed accommodation space S2 can be formed by the engagement of the first screwing portion and the second screwing portion 81. At this time, as shown in the first figure, the rotating shaft 3 and the pressing rod 4 are located in the cover body 7, and the driving element 5 is detachably sleeved on the cover body 7, and the bonding mode therebetween can be a card. The combination, or the component that was originally designed as a one-piece, is not intended to be limited to this creation. In addition, one of the outer sides of the cover body 7 can be designed to have a hook 9 which is convenient for the preparation of the stem cell preparation device of the present invention, but the present invention is not intended to be limited thereto. The sifting unit 1 and the squeezing unit 2 are detachably mounted on the accommodating unit 8 and preferably close to the upper opening of the accommodating unit 8. The erection mode can be detached or other types can be disassembled at any time. The way of fixing, this creation is equally not intended to be limited. Therefore, when the portion of the sample that has passed through the sifting unit 1 is cut by the cutting unit 2 and separated from the sample, it is dropped into the accommodating space S2 of the accommodating unit 8.

As described above, the rotating shaft 3, the pressing rod 4, the sifting unit 1 and the cutting unit 2 have through-holes H, respectively, and the intermediate through-holes H communicate with each other. Therefore, the stem cell preparation preparation device 100 of the present invention further comprises at least a sealing unit 6, and the material of the sealing unit 6 is preferably a recoverable silicone material. As shown in the first figure, since the middle through hole outlet of the cutting unit 2 is in communication with the accommodating space S2, one of the two sides of the middle through hole H of the rotating shaft 3 is connected to the middle through hole H of the pressing rod 4. Therefore, the sealing unit 6 is disposed on the other side of the through hole H in the rotating shaft 3 so that the entire stem cell preparation preparation device 100 is in a sealed state after being assembled. In the preferred embodiment, the sealing unit 6 includes a waterproof sheet 61 and a water blocking sheet 62, and the liquid can be injected into the accommodating space S2 after being punctured by an object such as a needle or a straw.

The composition and combination of the units of the stem cell preparation preparation device 100 provided by the present invention have been described in detail as before, and the operation mode of the above device will be further described later by the fourth diagram. First, please refer to the fourth figure, and with the first figure, the second figure and the third figure, when the organ tissue (ie, sample T) is cut, the collecting unit 8 for collection is erected, and the cutting unit is sequentially arranged. 2 and the sifting unit 1, the organ tissue T is placed in the groove S1 of the sifting unit 1, and then the lid 7 to which the rotating shaft 3, the pressing rod 4 and the sealing unit 6 have been integrated is screwed. Finally, a driving member 5 including a rotating sleeve 51, a pressing seat 52, and a spring 53 is fitted over the lid body 7. The driving element 5 is driven by finger pressing. At this time, the driving element 5 drives the rotating shaft 3 to push the pressing rod 4, and the pressing rod 4 is pressed toward the sifting unit 1, and the external force F is applied to the organ tissue T below it. .

Then, under appropriate pressure, part of the organ tissue will pass through the stencil unit 1 through the circular hole 11 having the predetermined size R. At this time, the organ tissue that has passed out has been shaped, and then cut by the lower cutting unit 2. The piece is cut, and the cut granular tissue t is collected and stored in the accommodating unit 8 according to the natural gravity rule.

Finally, when the cutting is completed to remove the driving element 5 (if the driving element also has a medium through hole, it may not be removed), the enzyme solution or the buffer solution may be sucked through the needle or the pipette, and then injected by the upper sealing unit 6 and then injected. In the accommodating space S2 of the accommodating unit 8, a subsequent reaction such as cell culture or the like is performed by mixing with the diced granular tissue t.

In summary, the dry cell preparation preparation device provided by the present invention can adjust the particle size specification of the cut object, that is, the soft and irregular texture can be shaped through the circular multi-aperture sieve-drain unit 1. The subsequent cutting to obtain a granular structure will effectively adjust the fineness of the tissue, overcoming the problem that the homogenizing device can only whipped the structure of powder, mud, juice and the like.

In addition, since the cutting unit 2 is disposed under the sifting unit 1, the cut tissue is collected and stored in the accommodating unit 8 according to the natural gravity rule, so as to avoid the tissue being subjected to repeated crushing and whipping. The cell death solves the problem that the conventional repetitive homogenization causes damage to the cells.

Finally, the driving element 5 is changed to the pressing mode, so that the stem cell preparation preparation device 100 of the present invention can achieve the convenience of one-hand operation, and further shorten the operation time and increase the experimental reproducibility, so that the cells separated by each cut are separated. Or tissue samples to achieve consistent and sterile infection effects.

The detailed description above is a detailed description of one of the possible embodiments of the present invention, and the embodiment is not intended to limit the scope of the patents, and the equivalent implementations or modifications that are not included in the spirit of the present invention should be included in The scope of this creation patent.

100‧‧‧Stem cell preparation preparation device

1‧‧‧Draining unit

2‧‧‧Cutting unit

21‧‧‧ cutting piece

3‧‧‧Rotary axis

31‧‧‧Upper joint

32‧‧‧ Lower joint

4‧‧‧Press

5‧‧‧Drive components

51‧‧‧Rotary sleeve

511‧‧‧Spiral guide

512‧‧‧Reset guide channel

513‧‧‧ recessed hole

52‧‧‧Press seat

521‧‧‧Medium hole

522‧‧‧Bumps

53‧‧‧ Spring

6‧‧‧ Sealing unit

61‧‧‧Waterproof tablets

62‧‧‧Water Block

7‧‧‧ Cover

8‧‧‧ housing unit

81‧‧‧Second screw joint

9‧‧‧hook

Claims (10)

A device for preparing a stem cell preparation for performing the same cutting, the device comprising: a driving component, the driving component comprising a rotating sleeve, a pressing seat and a spring, the rotating sleeve is sleeved with the pressing seat, and the spring Located between the pressing seat and the rotating sleeve, the outer peripheral wall of the rotating sleeve is provided with at least one spiral guiding groove, the bottom end of the spiral guiding groove is connected with an upward reset guiding groove, and the reset guiding groove is connected Adjacent to the spiral guide groove; a rotating shaft, the rotating shaft comprises an upper joint and a lower joint, the upper joint is connected with the rotating sleeve, the lower joint is connected with a pressing rod; a screen leakage unit, the screen leakage unit has Having the sample and a groove of the pressing rod, the bottom of the groove has at least one hole; a cutting unit, the cutting unit is adjacent to the sifting unit; a accommodating unit, the accommodating unit sifting The unit is detachably mounted in the accommodating unit, and a cover body is closely fitted to the accommodating unit, and has a hook on an outer side thereof. The apparatus for preparing a stem cell preparation according to claim 1, wherein the sample is placed between the pressing rod and the sifting unit, and when the driving element drives the rotating shaft to rotate and drives the pressing rod to apply When the external force is applied to the sample, the hole through which the sample portion passes through the sifting unit is cut by the cutting unit and separated from the sample, and then dropped into the accommodating unit. The stem cell preparation preparation device according to claim 2, wherein the sample piercing portion is cut into a granular structure after being cut by the cutting unit. The apparatus for preparing a stem cell preparation according to claim 3, wherein the hole of the sieve unit is circular and has a predetermined size, and the granular structure has the predetermined size. The apparatus for preparing a stem cell preparation according to claim 1, wherein the rotating shaft and the pressing rod are located in the cap body. The apparatus for preparing a stem cell preparation according to claim 1, wherein the rotating shaft, the pressing rod, the sifting unit and the cutting unit respectively have a through hole therein, and the middle through holes communicate with each other to A liquid can enter the accommodating unit through the middle through holes. The stem cell preparation preparation device according to claim 6, further comprising at least one sealing unit, wherein the sealing unit is disposed at one side of the middle through hole of the rotating shaft to seal the middle through hole. The stem cell preparation preparation device according to claim 1, wherein the cutting unit comprises a cutting piece. The apparatus for preparing a stem cell preparation according to claim 1, wherein the sample is derived from a biological tissue. The apparatus for preparing a stem cell preparation according to claim 9, wherein the biological tissue is a brain tissue, a liver tissue, a lung tissue, a muscle tissue or a fat tissue.