KR20240076670A - Biological sample collection and separation and quantitative injection device - Google Patents

Abstract

The present invention relates to a biological sample collection, separation, and quantitative injection device, which detects a biological sample (MB) from a specific area of a patient, then separates it into tissue separation cells (MB') by a control program, and separates the separated tissue separation cells (MB') from a specific area of the patient. MB') can be injected in a fixed amount into the patient's treatment area for optimal adhesion.
The biological sample collection, separation, and quantitative injection device according to the present invention inserts and fixes the needle 181 for collecting biological samples or the needle 171 for injecting tissue separation cells, and when collecting and injecting biological samples (MB), A cone-shaped needle fixing cap (540) equipped inside with a sterilizing lamp (400) for preventing infection that irradiates sterilizing light to the collection and injection site; The needle fixing cap 540 is fastened to one side, and a start processing button 410 is disposed on the outer side to control collection, separation, and quantitative injection operations of the biological sample (MB), and the start processing button 410 A cylindrical lower body 530 equipped therein with a biological sample tissue separation device 100 that collects and separates a biological sample (MB) and stores the separated tissue separation cells (MB') and injects them in a quantitative amount; A cylindrical middle body ( 520); And one side is fastened to the other side of the intermediate body 520, the separation lever 550 is fastened to the other side, the power button 360, and the collection of the biological sample (MB) according to the biological sample (MB) in the treatment area. A function setting button 370 for setting the separation and quantitative injection functions, displaying the collection, separation, and quantitative injection operations of the biological sample (MB) on the screen, and the biological sample tissue measured by the rotation speed meter 320 The number of rotations of the rotation axis 126 of the separation device 100 and the internal temperature of the cylindrical chamber 110 of the biological sample tissue separation device 100 measured by the temperature sensor 330 and the temperature measured by the pressure sensor 340 A display screen 380 that displays in real time the pressure applied to the porous plate 140 when the piston 128 of the cylindrical chamber 110 descends, and the collection of biological samples (MB) stored in the memory 310. A cylindrical upper body ( 510); In the biological sample collection, separation, and quantitative injection device, the biological sample collection, separation, and quantitative injection device includes an upper portion of the porous plate 140 inside the chamber of the biological sample tissue separation device 100 disposed therein. After loading the biological sample (MB), a certain amount of saline solution is supplied, and the biological sample (MB) is transferred to the porous plate 140 by the pressure of the piston 128 inside the chamber of the biological sample tissue separation device 100. The biological sample tissue separation device 100 has a cylindrical shape with a space formed inside and an opening at the bottom, and is separated into tissue separation cells (MB') by passing through the microhole 141. An upper body 120 on which a flange 121 is formed and a female screw fixing part 125 is formed at the center of the upper surface, and which is coupled to the upper body 120 and has a cylindrical shape with a space formed inside and an open upper part. A flange 131 is formed around the lower body 130, the inner lower surface of which is inclined toward the central outlet 136, and the needle insertion part 138 connected to the outlet 136 is protruding from the lower part. Equipped with a cylindrical chamber (110); A piston 128 that reciprocates within the upper body 120; It is vertically connected to the piston 128, and the male thread forming portion 127 formed on the outside engages with the female thread of the female thread fixing portion 125 to convert forward/reverse rotation into vertical reciprocating motion, thereby rotating the piston 128. A rotating shaft 126 that raises and lowers; It is disposed between the flange 121 of the upper body 120 and the flange 131 of the lower body 130, and a plurality of micro holes 141 are formed through the entire surface vertically, and the micro holes on the lower surface are A porous plate 140 formed smaller than the micropores on the upper surface; A saline solution supply pipe 122 formed inside the side of the upper body 120 and supplying saline solution to the inner space of the upper body 120 through the saline solution inlet 123 on the upper surface; and a biological sample supply pipe 133 formed inside the side surfaces of the upper and lower bodies 120 and 130, and for injecting a biological sample (MB) into the inner space of the upper body 120 through the needle insertion part 138. Includes ;

Description

Biological sample collection and separation and quantitative injection device}

The present invention relates to a biological sample collection, separation and quantitative injection device, and more specifically, to a biological sample (MB) control program, which collects a biological sample (MB) from a specific area of a patient and converts it into tissue separation cells (MB'). It relates to a biological sample collection, separation, and quantitative injection device that separates and injects a quantitative amount of tissue separation cells (MB') into the patient's treatment area to ensure optimal survival.

Today, the decomposition of biological tissues or materials such as human, animal, and plant tissue is known for a variety of purposes and applications.

Biological material (MB) may be dissected to perform medical tests, such as biopsies, or may be used for research purposes or to confirm a diagnosis as part of special medical treatment. It can also be broken down to obtain samples and specimens that can be examined and later analyzed in a laboratory to obtain data and information about the same sample.

In the prior art, a biological sample (MB) may be disaggregated for the purpose of isolating cells present in the same biological sample (MB) or for the purpose of preparing tissue pieces or micrografts to be used in clinical treatment and regenerative therapy. A micrograft is of subclinical size and is understood as a set of cells in the extracellular matrix that are visible under a microscope and not visible to the unaided eye.

Figure 1 is a diagram illustrating the typical structure of biological tissue before decomposition and schematically shows a typical biological sample (MB) consisting of biological tissue in its integrated form before being affected by decomposition operations.

As can be seen in Figure 1, biological tissue or material (MB), in its intact and undecomposed form, typically has a number of cells, denoted as cells (CEL), each of which is schematized with a number of parallel lines. It has its own nucleus (NU) and cytoplasm (CT) located within the extracellular matrix (MAT).

The biological sample (MB) is associated with cells (CEL), within the respective extracellular matrix (MAT). For example, growth factors further include substances and components composed of extracellular proteins and mineral components. In particular, these growth factors and substances (FC) are indicators of the cells' viability, i.e. their ability to grow and respond to external conditions, and are essential for cells (CEL) to exhibit their full properties and functions.

In the prior art, a biological sample (MB) is digested for tissue examination or analysis of cellular composites or to obtain samples of the same biological sample (MB). Systems and devices for decomposing such biological samples (MB) have been disclosed in the prior art.

Among them, International Application No. PCT/IB2015/059571 (hereinafter referred to as 'prior art document') discloses 'Device for decomposing biological samples and corresponding manufacturing method and method for preparing cell suspension and tissue micrograft'.

As shown in FIG. 2, the biological sample decomposition device includes an inner chamber 12 including an upper loading chamber 12a for loading a biological sample to be decomposed (MB), and an inner upper portion of the inner chamber 12. A cylindrical hollow outer body 11 that is stored in (11a) and collects the decomposed biological sample (MB) in the lower collection chamber 12b of the lower part 11b, and a cylindrical hollow outer body 11 below the inner chamber 12. A porous plate or decomposition grid 13 that is stored in the direction and has micropores 13a with sharp edges formed therein and decomposes the biological sample (MB) through the micropores 13a, and the internal chamber ( 12) a blade rotor (14) that rotates within the biological sample (MB) contained in the upper loading chamber (12a) to pass through the microholes (13a) of the decomposition grid (13) to decompose the biological sample (MB) It includes, and the micropores (13a) of the porous decomposition grid (13) have a dimension or diameter (D) of between 70 and 80㎛.

The device for decomposing biological samples in the prior art document extracts biological cells from a specific part of the patient using a syringe, collects them on a cloth or cotton cloth, and then removes skin tissue that is unnecessary for biological cells by manually trimming them one by one using tweezers and a scalpel. , is loaded inside the inner chamber (12). Afterwards, the cover 15 is closed, a motor is connected to the shaft 14a, and the biological sample MB is pulverized by rotating the distribution blade 14b and the lower scraper 14c. As the biological cells pass through the microholes 13a of the porous plate 13 by rotating the distribution blade 14b, the biological sample MB is pulverized and collected in the lower collection chamber 12b. Here, the lower scraper 14c rotates with the shaft 14a and serves to scrape the pulverized biological sample (MB) coming from the microhole 13a of the distribution blade 14b and collect it in the lower collection chamber 12b. do.

However, the apparatus for decomposing biological samples in the prior art literature has the following problems.

1) Since biological cells are extracted one by one from a specific part of the patient with a syringe and then temporarily collected on a strainer or cotton cloth, there is a high risk of contamination with bacteria, and if the surgery time is long, there is a problem of the biological cells being damaged or deteriorated.

2) The biological cells temporarily collected on the strainer or cotton cloth during the surgery are suctioned again using a syringe and then loaded inside the internal chamber (12), so the biological cells are highly likely to be contaminated with bacteria, and the procedure takes a lot of time and is cumbersome. There is a problem.

3) Hair follicles separated from the scalp still have unnecessary skin tissue around the hair follicles, including the epidermis layer, dermis layer, and first fat layer, so the hair follicle separator manually trims them one by one using tweezers and a scalpel to remove unnecessary skin tissue from the hair follicles. Remove. At this time, there was the inconvenience of having to clean up the area around the hair follicles to make it suitable for transplantation by removing unnecessary skin tissue attached to the hair follicles while the hair follicle separator frequently supplied saline solution to prevent the hair follicles from drying out.

4) When the distribution blade 14b rotates at high speed, there is a problem in that a large amount of biological cells are destroyed or separated by the pulverizing blade of the blade.

5) There is a problem that biological cells are destroyed or damaged due to frictional heat generated when the distribution blade 14b and the lower scraper 14c rotate at high speed.

6) Since the cover 15 is structured so that external air always penetrates into the inner chamber 12 when the cover 15 is opened and closed, there is a possibility that biological cells may be contaminated by bacteria.

7) The biological sample decomposition device has a length (or size) of less than 4 to 5 cm, but has a complicated internal structure and a large number of parts, which increases manufacturing costs. For example, the biological sample decomposition device includes a cover 15, an inner chamber 12, a shaft 14a, a distribution blade 14b, a porous plate 13, a lower scraper 14c, and a lower collection chamber 12b. Since it includes a hollow external body 11, the configuration is too complicated and the cost of replacement and repair of parts increases.

8) Depending on the biological sample (MB), the distance and pressure between the distribution blade 14b and the lower scraper 14c rotated by the shaft 14a and the porous plate 13 must be adjusted, and the distribution blade 14b There is also a problem of controlling the rotation speed.

9) Since the grinding blade of the distribution blade 14b becomes dull over time, there is a problem that it must be replaced at regular intervals.

10) Since the biological sample (MB) must be decomposed in a biological sample decomposition device and then the decomposed tissue isolation cells (MB') must be injected into the patient's treatment area using a syringe, it is vulnerable to bacterial infection and the procedure is complicated. There is a problem that the procedure takes a lot of time.

11) Since the biological sample decomposition device crushes the biological sample (MB) and then injects it into the patient's treatment area using a syringe, the amount injected is different each time, making it difficult to inject a fixed amount.

In addition, the biological sample decomposition devices currently in use in Korea are all imported products developed in foreign countries, so the product price is high and after service is difficult in case of failure.

Therefore, there is a need for local production of devices or systems that extract and decompose (or separate) biological samples (MB) and implant them, and for this purpose, continuous research and development is necessary.

In addition, the medical device industry is a promising future industry that will continue to grow as long as humanity exists. It is expected to expand in size and scope simultaneously with technological innovation, so domestic research and development to dominate technology is urgently needed.

International application number PCT/IB2015/059571 (International application date: 2015.12.14.) Domestic Public Patent No. 10-2021-0078460 (Publication Date: 2021.06.28.)

The technical problem to be achieved by the present invention in order to solve the above-mentioned problems is to collect a biological sample (MB) from a specific part of a patient and then separate tissue cells (MB') by a control program corresponding to the collected biological cells (MB). The purpose is to present a biological sample collection, separation, and quantitative injection device that separates tissue cells (MB') and injects them into the patient's treatment area in a quantitative amount to ensure optimal adhesion.

In addition, another technical problem to be achieved by the present invention is to extract autologous hair follicle cells from the scalp with many progenitor cells behind the ears of a patient with little hair loss, and then separate them into tissue separation cells (MB') and then inject them through a syringe. The purpose is to present a biological sample collection, separation, and quantitative injection device that treats hair loss by injecting it into the area requiring treatment.

In addition, another technical problem to be achieved by the present invention is to prevent contamination or bacterial infection and prevent cells from being damaged or deteriorated by collecting and separating biological samples (MB) in a closed space from the outside and injecting them in a quantitative amount. The purpose is to present a collection, separation and quantitative injection device.

In addition, another technical problem to be achieved by the present invention is to collect and separate biological samples (MB) in a closed space from the outside and inject quantitatively, thereby generating dermal papilla (Keratinocyte) and progenitor cells ( The purpose is to present a biological sample collection separation and quantitative injection device that prevents stable separation and damage to precursor cells.

In addition, another technical problem that the present invention aims to achieve is that collection of biological samples (MB), cell separation, and quantitative injection are performed quickly and accurately, and the procedure is simple and the procedure time is greatly reduced compared to the existing method. The purpose is to present a quantitative injection device.

In addition, another technical problem to be achieved by the present invention is to prevent the problem of biological cells being destroyed or damaged by the grinding blade by collecting and separating biological samples (MB) and injecting them in a fixed amount using the pressure of a piston in a space sealed from the outside. The purpose is to present a biological sample collection, separation, and quantitative injection device.

In addition, another technical problem to be achieved by the present invention is to present a biological sample collection, separation, and quantitative injection device that is simple in construction, unlikely to break down, and can be used semi-permanently without replacing parts.

In addition, another technical problem to be achieved by the present invention is to propose a biological sample collection, separation, and quantitative injection device that separates the cell tissue of a biological sample (MB) according to the characteristics of the treatment area.

In addition, another technical problem to be achieved by the present invention is to propose a biological sample collection, separation, and quantitative injection device that separates cell tissue according to the type and state of the biological sample (MB).

In addition, another technical problem to be achieved by the present invention is to present a biological sample collection, separation, and quantitative injection device that provides an efficient and innovative treatment method without pain and scars using a non-surgical treatment method.

In addition, another technical problem to be achieved by the present invention is to propose a lightweight, all-in-one biological sample collection, separation, and quantitative injection device to minimize infection and cell damage and to ensure optimal adhesion during transplantation of tissue isolation cells (MB'). There is a purpose.

In addition, another technical problem to be achieved by the present invention is to present a new concept of biological sample collection, separation, and quantitative injection device using vacuum compression technology as a non-surgical treatment method.

In addition, another technical problem to be achieved by the present invention is to provide a biological sample collection, separation, and quantitative injection device that allows easy cleaning of the interior of the chamber and replacement of parts.

The problems to be solved by the present invention are not limited to those mentioned above, and other problems not mentioned can be clearly understood by those skilled in the art from the description below.

As a means to solve the above-described technical problem, the biological sample collection, separation, and quantitative injection device according to the present invention includes, as described in claim 1, a needle 181 for collecting biological samples or a needle 181 for injecting tissue separation cells ( 171) is inserted and fixed, and a cone-shaped needle fixing cap 540 is provided inside with a sterilizing lamp 400 for preventing infection that irradiates sterilizing light to the collection and injection site when collecting and injecting a biological sample (MB). ; The needle fixing cap 540 is fastened to one side, and a start processing button 410 is disposed on the outer side to control collection, separation, and quantitative injection operations of the biological sample (MB), and the start processing button 410 A cylindrical lower body 530 equipped therein with a biological sample tissue separation device 100 that collects and separates a biological sample (MB) and stores the separated tissue separation cells (MB') and injects them in a quantitative amount; A cylindrical middle body ( 520); And one side is fastened to the other side of the intermediate body 520, the separation lever 550 is fastened to the other side, the power button 360, and the collection of the biological sample (MB) according to the biological sample (MB) in the treatment area. A function setting button 370 for setting the separation and quantitative injection functions, displaying the collection, separation, and quantitative injection operations of the biological sample (MB) on the screen, and the biological sample tissue measured by the rotation speed meter 320 The number of rotations of the rotation axis 126 of the separation device 100 and the internal temperature of the cylindrical chamber 110 of the biological sample tissue separation device 100 measured by the temperature sensor 330 and the temperature measured by the pressure sensor 340 A display screen 380 that displays in real time the pressure applied to the porous plate 140 when the piston 128 of the cylindrical chamber 110 descends, and the collection of biological samples (MB) stored in the memory 310. A cylindrical upper body ( 510); In the biological sample collection, separation, and quantitative injection device, the biological sample collection, separation, and quantitative injection device includes an upper portion of the porous plate 140 inside the chamber of the biological sample tissue separation device 100 disposed therein. After loading the biological sample (MB), a certain amount of saline solution is supplied, and the biological sample (MB) is transferred to the porous plate 140 by the pressure of the piston 128 inside the chamber of the biological sample tissue separation device 100. The biological sample tissue separation device 100 has a cylindrical shape with a space formed inside and an opening at the bottom, and is separated into tissue separation cells (MB') by passing through the microhole 141. An upper body 120 on which a flange 121 is formed and a female screw fixing portion 125 is formed at the center of the upper surface, and which is coupled to the upper body 120 and has a cylindrical shape with a space formed inside and an open upper part. A flange 131 is formed around the lower body 130, the inner lower surface of which is inclined toward the central outlet 136, and the needle insertion part 138 connected to the outlet 136 is protruding from the lower part. Equipped with a cylindrical chamber (110); A piston 128 that reciprocates within the upper body 120; It is vertically connected to the piston 128, and the male thread forming portion 127 formed on the outside engages with the female thread of the female thread fixing portion 125 to convert forward/reverse rotation into vertical reciprocating motion, thereby rotating the piston 128. A rotating shaft 126 that raises and lowers; It is disposed between the flange 121 of the upper body 120 and the flange 131 of the lower body 130, and a plurality of micro holes 141 are formed through the entire surface vertically, and the micro holes on the lower surface are A porous plate 140 formed smaller than the micropores on the upper surface; A saline solution supply pipe 122 formed inside the side of the upper body 120 and supplying saline solution to the inner space of the upper body 120 through the saline solution inlet 123 on the upper surface; and a biological sample supply pipe 133 formed inside the side surfaces of the upper and lower bodies 120 and 130 and for injecting a biological sample (MB) into the internal space of the upper body 120 through the needle insertion part 138. It is characterized by a biological sample collection, separation and quantitative injection device including;

As described in claim 2, the biological sample tissue separation device 100 includes a first on/off valve 124 disposed in the saline solution supply pipe 122 and controlling the supply or blocking of the saline solution; second and third opening/closing valves 134 and 135 disposed at both ends of the biological sample supply pipe 133 and controlling the supply or blocking of the biological sample MB; A fourth on/off valve 137 disposed at the outlet 136 and controlling discharge of the separated tissue separation cells (MB'); a memory 310 storing control programs for collection, separation, and quantitative injection of the biological sample (MB) according to the type of the biological sample (MB); And according to the control program for collection, separation, and quantitative injection of the biological sample (MB) stored in the memory 310, the rotation speed of the rotation axis 126 is adjusted to inject the biological sample (MB) in a quantitative amount, and the biological sample (MB) is When collecting MB), the first to third on/off valves 124, 134, 135 are opened and the fourth on/off valve 137 is closed, and when separating and quantitatively injecting the biological sample (MB), the first to third on/off valves 124, 134, and 135 are opened. It is characterized by a biological sample microimplantation method including a control unit 440 that closes the on-off valves 124, 134, and 135 and opens the fourth on-off valve 137.

According to the present invention, it is a non-surgical treatment method in which the patient's specific autologous cells are extracted and then separated, the isolated autologous cells are injected in a quantitative amount, and the cells self-differentiate and proliferate to treat the affected area. There is no pain and scarring, and treatment is improved. Because the effect is large, it has the effect of providing efficient and groundbreaking treatment to patients.

In addition, autologous micro-implantation technology allows treatment with a single injection within a short period of time, and is economically effective in saving both cost and time as well as excessive stress on the patient.

In addition, it can be applied to various medical fields, including hair loss treatment, knee joint treatment, periodontal disease treatment, and skin burn treatment, and can be applied to the aesthetic relief industry through body tissue micrografting.

In addition, it is possible to participate in the infrastructure project of surrounding medical equipment linked to the density of the suspension and the corresponding technology, and has the effect of contributing to the local production and overseas export of medical devices for producing biological hair follicle samples.

In addition, the supply of low-cost, high-efficiency medical devices can reduce personal and social costs for alopecia treatment, reduce stress caused by alopecia in individuals, and reduce the resulting damage.

In addition, medical institutions can provide quality medical services to consumers by supplying highly efficient medical devices.

In addition, it is possible to perform the procedure at low cost and in a short time, avoiding the high cost and long time required for hair transplantation, and it is easy to perform and recover anytime and anywhere, and the low cost allows anyone to perform the procedure without burden.

The effects of the present invention are not limited to those mentioned above, and other effects not mentioned will be clearly understood by those skilled in the art from the description below.

Figure 1 is a diagram showing a typical structure of biological tissue before decomposition.
Figure 2 is an exploded perspective view of a biological sample decomposition device disclosed in Korean Patent Publication No. 10-2017-0102479, which is a prior art document.
Figure 3 is a configuration diagram showing a biological sample collection, separation, and quantitative injection device according to an embodiment of the present invention.
Figure 4 is an operational flowchart showing a method of collecting, separating, and quantitatively injecting a biological sample according to an embodiment of the present invention.
Figures 5 to 8 are diagrams showing a biological sample collection, separation, and quantitative injection device 500;
Figure 5 is a perspective view of a fixed needle 181 for collecting biological samples;
Figure 6 is a perspective view of the injection needle 171 for tissue separation cell injection being fixed;
Figure 7 is an exploded perspective view of the biological sample collection, separation, and quantitative injection device 500;
Figure 8 is a block diagram of a biological sample collection, separation, and quantitative injection device 500.
9 to 12 are diagrams showing the biological sample tissue separation device 100 installed inside the biological sample collection, separation, and quantitative injection device 500.
Figure 9 is a perspective view,
Figure 10 is an exploded perspective view,
Figure 11 is a cross-sectional view,
Figure 10 is a cross-sectional view with the injection needle 171 for tissue separation cell injection inserted.
Figure 12 is a perspective view of the porous plate 140.
Figure 13 is a diagram showing a cell suspension obtained with a biological sample tissue separation device according to an embodiment of the present invention.

Below, with reference to the attached drawings, embodiments of the present invention will be described in detail so that those skilled in the art can easily implement the present invention. However, the present invention may be implemented in many different forms and is not limited to the embodiments described herein. In order to clearly explain the present invention in the drawings, parts unrelated to the description have been omitted, and similar parts will be described with similar reference numerals throughout the description of the invention.

Hereinafter, specific technical details to be implemented in the present invention will be described in detail with reference to the accompanying drawings.

First, the biological sample collection, separation, and quantitative injection device according to the present invention is suitable and advantageous for the preparation of cell suspensions and tissue micrografts, and tissue isolation cells (MB') are used for subclinical use. It is a cell suspension or set of cells within a matrix of cells that are not visible to the naked eye but visible under a microscope.

A biological sample (MB) is a typical biological tissue composed of biological tissue in its integrated form before being affected by decomposition operations and has the structure shown in Figure 1.

As can be seen in Figure 1, a biological tissue or material, in its intact and undecomposed form, typically has a number of cells, denoted as cells (CELs), each of which contains an extracellular matrix schematized by a number of parallel lines. It has its own nucleus (NU) and cytoplasm (CT) located within (MAT).

The biological sample (MB) is associated with cells (CEL), within the respective extracellular matrix (MAT). For example, growth factors further include substances and components composed of extracellular proteins and mineral components. In particular, these growth factors and substances (FC) are indicators of the cells' viability, i.e. their ability to grow and respond to external conditions, and are essential for cells (CEL) to exhibit their full properties and functions.

For reference, Figure 13 is a diagram showing a cell suspension obtained with the biological sample tissue separation device according to the present invention, its own nucleus (NU) and cytoplasm (CT) and growth factors and substances located in the extracellular matrix (MAT) Contains field (FC).

Biological sample collection separation and quantitative injection device (1000)

Figure 3 is an operational concept diagram showing a biological sample collection, separation, and quantitative injection device according to an embodiment of the present invention, and Figure 4 is an operational flow chart showing a biological sample collection, separation, and quantitative injection method.

Referring to FIGS. 3 and 4 , first, the needle 181 for collecting a biological sample is inserted and fixed into the biological sample collection, separation, and quantitative injection device 500 of the present invention. Then, a control program is selected through the function setting button 370 of the biological sample collection, separation and quantitative injection device 500 according to the type of biological sample (MB) according to the patient's treatment area. At this time, the control program controls the operation of the biological sample collection, separation and quantitative injection device 500 according to the characteristics of the treatment area to collect and separate the biological sample (MB) and inject a quantitative amount.

Here, the configuration and operation of the biological sample collection, separation, and quantitative injection device 500 will be described in detail in FIGS. 5 to 12, which will be described later.

Next, set the part of the patient's body from which to collect the biological sample (MB), perform partial anesthesia, hold the biological sample collection separation and quantitative injection device 500 by hand, and use the needle 181 for collecting the biological sample. After insertion, the anesthetized area is pumped to collect a biological sample (MB). At this time, when the start processing button 410 of the biological sample collection separation and quantitative injection device 500 is pressed, the drive motor 350 is operated by the control program and the anesthetized area is moved through the suction operation of the piston 128. A biological sample (MB) is collected by pumping.

The biological sample (MB) collected from the biological sample collection, separation, and quantitative injection device 500 is stored inside the chamber of the biological sample tissue separation device 100 disposed inside the biological sample collection, separation, and quantitative injection device 500. It is loaded on top of the porous plate 140. When a biological sample (MB) is loaded on the porous plate 140, a certain amount of saline solution is automatically sprayed on the biological sample (MB).

Next, the drive motor 350 operates according to the control program to lower the piston 128. At this time, inside the chamber of the biological sample tissue separation device 100, the biological sample MB is pushed through the microhole 141 of the porous plate 140 by the pressure of the piston 128. At this time, the biological sample (MB) passes through the micropores 141 of the porous plate 140 and is separated into tissue separation cells (MB') and loaded.

Next, the needle 171 for tissue separation cell injection is inserted and fixed in the biological sample collection, separation, and quantitative injection device 500, and then injected into the treatment area of the patient to inject a quantitative amount of the tissue separation cells (MB'). do. At this time, quantitative injection of the tissue separation cells (MB') is possible by adjusting the rotation speed of the drive motor 350 and the rising and falling speed of the piston 128 by the control program.

Hereinafter, the non-surgical integrated biological sample collection, separation, and quantitative injection device 500 according to the present invention will be described with reference to the attached drawings.

Embodiment of biological sample collection separation and quantitative injection device 500

Figures 5 to 8 are diagrams showing a biological sample collection separation and quantitative injection device 500. Figure 5 is a perspective view of a fixed needle 181 for collecting biological samples, and Figure 6 is a diagram showing a device for injecting tissue separation cells. It is a perspective view of the injection needle 171 being fixed, Figure 7 is an exploded perspective view of the biological sample collection, separation and quantitative injection device 500, and Figure 8 is a block diagram of the biological sample collection, separation and quantitative injection device 500. am.

As shown in FIGS. 5 to 7, the non-surgical integrated biological sample collection, separation, and quantitative injection device 500 according to the present invention largely consists of an upper body 510, a middle body 520, a lower body 530, and a needle fixation device. It is distinguished by a cap (540).

The fixing cap 540 has a conical shape, and the female thread portion 541 formed on the other side is fastened to and fixed to the male thread portion 532 formed on one side of the lower body 530. The fixing cap 540 is fixed by inserting a needle 181 for collecting biological samples or a needle 171 for injecting tissue separation cells through a hole in the center.

The fixing cap 540 is made of a transparent or translucent light-transmitting material, and a sterilizing lamp 400 for preventing infection is placed therein. The anti-infection sterilizing lamp 400 serves to prevent infection and sterilize bacteria, etc. by irradiating sterilizing light to the collection and injection site of the patient when collecting and injecting a biological sample (MB).

The lower body 530 has a cylindrical shape, and the needle fixing cap 540 is fastened to one side. Male thread portions 531 and 532 are formed on one side and the other side of the lower body 530. However, depending on the shape of the screw formed on the needle fixing cap 540 or the intermediate body 520, either or both may be formed as female threads.

A start processing button 410 is disposed on the outer surface of the lower body 530 to control collection, separation, and quantitative injection operations of the biological sample (MB). And, inside the lower body 530, the biological sample (MB) is collected and separated by the start processing button 410, and the separated tissue separation cells (MB') are stored and injected in a fixed amount into the patient's treatment area. A biological sample tissue separation device 100 is included.

Here, the configuration and operation of the biological sample tissue separation device 100 will be described in detail with reference to FIGS. 9 to 12, which will be described later.

Subsequently, the middle body 520 has a cylindrical shape, and one side is fastened to the other side of the lower body 530. Female thread portions 521 are formed on one side and the other side of the intermediate body 520. However, depending on the shape of the screw formed in the lower body 530 and the upper body 510, one or both may be formed as male thread parts.

The intermediate body 520 may be provided with a saline solution motor 420 and a saline solution storage tank 430 therein. The saline motor 420 and the saline solution storage tank 430 serve to supply saline solution to the biological sample tissue separation device 100.

The upper body 510 has a cylindrical shape, one side is fastened to the other side of the middle body 520, and a separation lever 550 is fastened to the other side. A male thread portion 511 is formed on one side of the upper body 510. However, if a male thread is formed on the other side of the intermediate body 520, it may be formed as a female thread.

A power button 360, a function setting button 370, a display screen 380, and an operation indicator lamp 390 may be disposed on the outer surface of the upper body 510.

Here, the function setting button 370 sets the collection, separation, and quantitative injection functions of the biological sample (MB) according to the patient's treatment area and the type of the biological sample (MB).

The display screen 380 displays the collection, separation, and quantitative injection operations of the biological sample (MB) on the screen, and displays the number of rotations and internal temperature and pressure of the rotation shaft 126 of the biological sample tissue separation device 100. Displayed in real time. The operation display lamp 390 displays the collection, separation, and quantitative injection operations of the biological sample (MB) by lighting and blinking the lamp. The display screen 380 may be configured to display the count of separated tissue cells (MB').

Additionally, the upper body 510 may have a power supply unit 300, a memory 310, and a control unit 440 inside.

Here, the power supply unit 300 supplies power from a rechargeable battery or commercial power. The memory 310 stores control programs for collection, separation, and quantitative injection of biological samples (MB) according to the treatment area and type of biological sample (MB). At this time, the control program is programmed to automatically adjust the rotation speed of the rotation axis 126 of the biological sample tissue separation device 100 and the temperature and pressure inside the chamber according to the treatment area and the type of biological sample (MB). there is.

The control unit 440 automatically controls the operation of the biological sample tissue separation device 100 according to a control program for collection, separation, and quantitative injection of the biological sample (MB) stored in the memory 310. For example, the rotation speed, internal temperature, and pressure of the rotation shaft 126 of the biological sample tissue separation device 100 are adjusted, and the rotation speed of the rotation shaft 126 and the first to fourth opening and closing valves 124, 134, 135, and 137 are controlled. The movement is controlled to control the collection, separation, and quantitative injection of biological samples (MB).

For example, when collecting a biological sample (MB), the control unit 420 opens the first to third on/off valves (124, 134, 135) and closes the fourth on/off valve (137), and collects the biological sample (MB). During separation and quantitative injection, the first to third on/off valves 124, 134, and 135 are closed, the fourth on/off valve 137 is opened, and the rotation speed of the rotation shaft 126 is adjusted to prepare the biological sample (MB). Controls quantitative injection.

Additionally, the biological sample collection, separation, and quantitative injection device 500 may include a rotation speed meter 320, a temperature sensor 330, a pressure sensor 340, and a drive motor 350.

The rotation speed meter 320 measures the rotation speed of the rotation shaft 126 in real time to detect the rising and falling speed of the piston 128 inside the cylindrical chamber 110 of the biological sample tissue separation device 100. It will be measured. The rotation speed meter 320 may be configured in the cylindrical chamber 110.

The temperature sensor 330 serves to measure the internal temperature of the cylindrical chamber 110 in real time. In addition, the pressure sensor 340 serves to measure the pressure applied to the biological sample (MB) or the porous plate 140 in real time when the piston 128 descends. The temperature sensor 330 and the pressure sensor 340 may be configured inside the cylindrical chamber 110.

The drive motor 350 is a driving source that rotates the rotation shaft 126, and may be disposed in the lower body 530 together with the biological sample tissue separation device 100.

Hereinafter, the biological sample tissue separation device 100 will be described with reference to the accompanying drawings.

Embodiment of biological sample tissue separation device 100

9 to 12 are diagrams showing the biological sample tissue separation device 100 installed inside the biological sample collection, separation, and quantitative injection device 500, where FIG. 9 is a perspective view, FIG. 10 is an exploded perspective view, and FIG. 11 is a cross-sectional view, FIG. 10 is a cross-sectional view with the injection needle 171 for tissue separation cell injection inserted, and FIG. 12 is a perspective view of the porous plate 140. And Figure 13 is a diagram showing the cell suspension obtained with the biological sample tissue separation device according to the present invention.

As shown in FIGS. 9 to 12, the biological sample tissue separation device 100 according to the present invention includes a cylindrical chamber 110 largely composed of an upper body 120 and a lower body 130.

The upper body 120 has a cylindrical shape with a space formed inside and an open bottom, a flange 121 is formed around the bottom, and a female screw fixing part 125 is disposed or formed at the center of the upper surface. A biological sample (MB) to be decomposed is loaded in the internal space of the upper body 120.

The lower body 130 is coupled to the upper body 120 and has a cylindrical shape with a space formed inside and an open top, a flange 131 is formed around the top, and the inner lower surface has a central outlet ( 136). A needle insertion portion 138 is formed protruding from the lower part of the lower body 130, and the discharge port 136 passes through and is connected to the inside. In the internal space of the lower body 130, the biological sample MB is loaded by collecting tissue separation cells MB' separated through the micropores 141 of the porous plate 140.

The upper and lower bodies 120 and 130 have vents 139 formed on the inside of the side walls to allow air to pass through each other, and vents 129 are also formed through the upper part of the upper body 120. Because of this, the piston 128 can be smoothly raised and lowered inside the cylindrical chamber 110.

The vent 139 formed in the lower body 130 is located below the porous plate 140 and may be formed as a micro hole. In addition, a mesh or filter may be installed on the inner wall of the lower body 130 where the ventilation hole 139 is formed to prevent the tissue separation cells MB' from being discharged out through the ventilation hole 139.

Inside the upper body 120, the piston 128 makes a reciprocating motion while rising and falling vertically. The piston 128 has a disk shape and a rotation axis 126 is vertically connected to the top. The rotation shaft 126 is installed to rotate through the upper body 120 and the female screw fixing portion 125 disposed at the upper center of the upper body 120. At this time, the rotation shaft 126 is installed so that the male thread forming portion 127 formed on the outside of the rotating shaft 126 of a certain length engages with the female thread of the female thread fixing portion 125. Therefore, when the rotation shaft 126 rotates forward and backward, the male thread forming portion 127 engages with the female thread of the female thread fixing portion 125 and converts it into a vertical reciprocating motion to raise and lower the piston 128. .

The upper body 120 has a saline solution supply pipe or saline solution supply passage 122 formed on the inside of the side surface. One side of the saline water supply pipe or saline water supply passage 122 is connected to the saline water inlet 123 formed on the upper surface of the upper body 120, and the other side is connected to the internal space of the upper body 120. And a first on/off valve 124 is disposed on one side or the other side of the saline water supply pipe or saline water supply passage 122, and the upper body ( Saline solution is supplied to and blocked from the internal space of 120).

A porous plate 140 may be disposed between the flange 121 of the upper body 120 and the flange 131 of the lower body 130. Here, the porous plate 140 has a plurality of micro-holes 141 formed vertically penetrating the entire surface of the disk shape, and the size of the micro-holes becomes smaller from the upper surface to the lower surface.

Here, the microhole 141 may be formed to have a size or diameter between 60 and 80㎛. Preferably, it may be formed to have a dimension or diameter of 75㎛ or less. The shape of the microhole 141 may be a cone, triangular pyramid, square pyramid, pentagonal pyramid, hexagonal pyramid, or star pyramid.

For example, the conical microhole has a circular inlet formed on the upper surface of the porous plate 140 and a sharp edge-shaped outlet formed on the lower surface, and the outlet is formed smaller than the inlet.

Likewise, the star-shaped microhole has a star-shaped inlet formed on the upper surface of the porous plate 140 and a star-shaped outlet formed on the lower surface, and the outlet is formed smaller than the inlet.

The biological sample (MB) passes through the micropores 141 of the porous plate 140 and is separated into tissue separation cells (MB') having a size of 20 to 75 μm or less. For reference, Figure 14 shows the cell suspension of the tissue isolated cells (MB').

The material of the porous plate 140 may be made of stainless steel, metal, or alloy, and may be formed using a mold, die-punch, etc.

O-rings 150 and 151 may be disposed between the flange 121 of the upper body 120 and the porous plate 140 and between the porous plate 140 and the flange 131 of the lower body 130. The O-rings 150 and 151 may be formed of a circular ring, natural rubber, synthetic rubber, synthetic resin, etc., and serve as an air seal.

Subsequently, a biological sample supply pipe or a biological sample supply passage 133 is formed inside the side surfaces of the upper and lower bodies 120 and 130. One side of the biological sample supply pipe or biological sample supply passage 133 is connected to the internal space of the upper body 120 and the other side is connected to the outlet 136 of the lower body 130.

Second and third on/off valves 134 and 135 are disposed on one side or the other of the biological sample supply pipe or biological sample supply passage 133, and the biological sample is collected by opening and closing the second and third on/off valves 134 and 135. The biological sample (MB) is collected through the injection needle 181 and injected into the internal space of the upper body 120. At this time, the biological sample (MB) introduced into the internal space of the upper body 120 is loaded on the upper part of the porous plate 140.

The second and third on/off valves 134 and 135 are disposed at both ends of the biological sample supply pipe 133 and control the supply or blocking of the biological sample MB into the internal space of the upper body 120.

A fourth on/off valve 137 is disposed at the outlet 136 of the lower body 130. The fourth on/off valve 137 controls discharge of tissue separation cells (MB') separated while passing through the microholes 141 of the porous plate 140 through the outlet 136.

Examples of tissue isolation methods for biological samples

The method for separating tissue from a biological sample according to the present invention is described as follows.

First, a needle 181 for collecting a biological sample is fixed to the needle insertion portion 138 of the cylindrical chamber 110.

Next, after anesthetizing the biological sample collection part of the patient, the needle 181 for collecting the biological sample is inserted into the patient's body and then the piston 128 of the cylindrical chamber 110 is raised to inhale, A biological sample (MB) is collected through a biological sample collection needle 181 and placed on the upper porous plate 140 inside the upper body 120.

Next, saline solution is supplied to the biological sample (MB) loaded on the upper part of the porous plate 140 through the saline solution supply pipe 122.

Next, all of the first to fourth opening/closing valves 124, 134, 135, and 137 of the cylindrical chamber 110 are closed, and then pressure is applied while lowering the piston 128 so that the biological sample MB is moved to the porous plate 140. ) to pass through the fine hole 141. At this time, the upper and lower bodies 120 and 130 have vents 139 formed on the inside of the side walls to allow air to pass through each other, and vents 129 are also formed through the upper part of the upper body 120. Because of this, the piston 128 can be smoothly raised and lowered inside the cylindrical chamber 110.

The biological sample (MB) (see FIG. 1) passes through the micropores 141 of the porous plate 140 and is separated into tissue separation cells (MB') (see FIG. 15) and then stored inside the lower body 130. is loaded on

Next, the needle 171 for tissue separation cell injection is inserted into the needle insertion portion 138 of the cylindrical chamber 110 and fixed. And after closing the first to third on/off valves (124, 134, 135) and opening the fourth on/off valve (137), the tissue separation cell injection needle (171) is injected into the treatment area of the patient to separate the tissue. Cells (MB') are injected in a quantitative amount. At this time, the quantitative injection of the tissue separation cells (MB') is performed by adjusting the rotation speed of the rotation axis 126 to ensure constant quantitative injection.

The biological sample tissue separation device according to the present invention extracts biological cells from a specific area of the patient, separates them into tissue separation cells (MB'), and injects the separated tissue separation cells (MB') into the patient's treatment area in a quantitative amount, The injected tissue isolation cells (MB') can self-differentiate and proliferate to treat the affected area.

Figure 15 is a diagram showing a cell suspension obtained by the biological sample tissue separation device according to the present invention, showing its own nucleus (NU) and cytoplasm (CT) and growth factors and substances (FC) located in the extracellular matrix (MAT) ) is included.

As described above, the biological sample collection, separation, and quantitative injection device according to the present invention collects a biological sample (MB) from a specific area of the patient using the biological sample tissue separation device 100, and then tissue tissue by a control program. Separated into separated cells (MB') and injected in a fixed amount into the patient's treatment area to ensure optimal survival, the injected tissue isolated cells (MB') self-differentiate and proliferate to treat the affected area. By doing so, the technical problem of the present invention can be solved.

The preferred embodiments of the present invention described above have been disclosed to solve technical problems, and those skilled in the art can make various modifications, changes, additions, etc. within the spirit and scope of the present invention. This will be possible, and such modifications and changes should be considered as falling within the scope of the claims below.

The biological sample collection, separation, and quantitative injection device according to the present invention can be applied to various medical fields, including hair loss patients, arthritis patients, periodontal disease patients, and burn patients. In addition, it can also be applied to the aesthetic relief industry through body tissue microtransplantation. In addition, it can be applied to artificial bone heads, artificial bones, ophthalmic prosthetic devices, dental implants, maxillary bone reconstruction, degenerative knee joints, otolaryngology, etc.

100: Biological sample tissue separation device
110: cylindrical chamber 120: upper body
121: flange
122: Saline solution supply pipe or saline solution supply passage
123: Saline water inlet 124: First opening/closing valve
125: female screw fixing part 126: rotation axis
127: Male thread forming part 128: Piston
129: vent 130: lower body
131: Flange 132: Screw hole
133: Biological sample supply pipe or biological sample supply passage
134, 135: second and third opening and closing valves 136: outlet
137: Fourth opening/closing valve 138: Injection needle insertion portion
139: ventilation hole 140: porous plate
141: fine hole 142: screw insertion hole
150, 151: O-ring 160: Bolt
161: Nut 170: Syringe body
171: Injection needle for tissue separation cell injection
180: syringe body
181: Syringe needle for collecting biological samples
190: Extraction and injection separation pipe
200: conical fine hole 201: inlet
202: Sharp edge-shaped outlet 210: Triangular pyramid-shaped micro hole
211: inlet 212: sharp edge outlet
220: Square pyramid fine hole 221: Inlet
222: Sharp edge type outlet 230: Pentagonal pyramid shaped fine hole
231: Inlet 232: Sharp edge-shaped outlet
240: Star-shaped horn-shaped microhole 241: Inlet
242: Sharp edge outlet 300: Power unit
310: Memory 320: Rotation speed meter
330: temperature sensor 340: pressure sensor
350: Drive motor 360: Power button
370: Function setting button 380: Display screen
390: Operation indicator lamp 400: Sterilization lamp for infection prevention
410: Start Processing Button
420: Saline solution motor 430: Saline solution storage tank
440: control unit
500: Biological sample collection separation and quantitative injection device
510: upper body 511: male thread portion
520: Middle body 521: Female thread portion
530: lower body 531, 532: male thread portion
540: Needle fixing cap 541: Female thread part
550: Separator
1000: Biological sample collection separation and quantitative injection device
MB: temperament as an indicator of survival ability
MB': biological sample digested in cell suspension (MB)
CEL: Tissue isolated cells (MB')

Claims (2)

Insert and secure the needle 181 for collecting biological samples or the needle 171 for injecting tissue separation cells, and a sterilizing lamp for infection prevention that irradiates sterilizing light to the collection and injection site when collecting and injecting biological samples (MB). A cone-shaped needle fixing cap 540 provided inside (400); The needle fixing cap 540 is fastened to one side, and a start processing button 410 is disposed on the outer side to control the collection, separation, and quantitative injection operations of the biological sample (MB), and the start processing button 410 A cylindrical lower body 530 equipped therein with a biological sample tissue separation device 100 that collects and separates a biological sample (MB) and stores the separated tissue separation cells (MB') and injects them in a quantitative amount; A cylindrical middle body ( 520); And one side is fastened to the other side of the intermediate body 520, the separation lever 550 is fastened to the other side, the power button 360, and the collection of the biological sample (MB) according to the biological sample (MB) in the treatment area. A function setting button 370 for setting the separation and quantitative injection functions, displaying the collection, separation, and quantitative injection operations of the biological sample (MB) on the screen, and the biological sample tissue measured by the rotation speed meter 320 The number of rotations of the rotation axis 126 of the separation device 100 and the internal temperature of the cylindrical chamber 110 of the biological sample tissue separation device 100 measured by the temperature sensor 330 and the temperature measured by the pressure sensor 340 A display screen 380 that displays in real time the pressure applied to the porous plate 140 when the piston 128 of the cylindrical chamber 110 descends, and the collection of biological samples (MB) stored in the memory 310. A cylindrical upper body ( 510); In the biological sample collection, separation and quantitative injection device comprising,
The biological sample collection separation and quantitative injection device,
After loading a biological sample (MB) on the upper part of the porous plate 140 inside the chamber of the biological sample tissue separation device 100 disposed therein, a certain amount of saline solution is supplied, and the chamber of the biological sample tissue separation device 100 is supplied. The biological sample (MB) is passed through the microholes 141 of the porous plate 140 using the pressure of the piston 128 from the inside, and is separated and loaded into tissue separation cells (MB'),
The biological sample tissue separation device 100,
An upper body 120 having a cylindrical shape with a space formed inside and an open lower part, a flange 121 formed around the bottom, and a female screw fixing part 125 formed at the center of the upper surface, the upper body 120 and It is combined and has a cylindrical shape with a space formed inside and an open top, a flange 131 is formed around the top, the inner bottom is inclined toward the central outlet 136, and the outlet 136 is connected to the inside. A cylindrical chamber 110 having a lower body 130 with a needle insertion portion 138 protruding from the lower portion;
A piston 128 that reciprocates within the upper body 120;
It is vertically connected to the piston 128, and the male thread forming portion 127 formed on the outside engages with the female thread of the female thread fixing portion 125 to convert forward/reverse rotation into vertical reciprocating motion, thereby rotating the piston 128. A rotating shaft 126 that raises and lowers;
It is disposed between the flange 121 of the upper body 120 and the flange 131 of the lower body 130, and a plurality of micro holes 141 are formed through the entire surface upward and downward, and the micro holes on the lower surface are A porous plate 140 formed smaller than the micropores on the upper surface;
A saline solution supply pipe 122 formed inside the side of the upper body 120 and supplying saline solution to the inner space of the upper body 120 through the saline solution inlet 123 on the upper surface; and
A biological sample supply pipe 133 formed inside the side surfaces of the upper and lower bodies 120 and 130 and injecting a biological sample (MB) into the internal space of the upper body 120 through the needle insertion part 138;
A biological sample collection, separation and quantitative injection device comprising: In claim 1,
The biological sample tissue separation device 100,
A first on/off valve 124 disposed in the saline water supply pipe 122 and controlling the supply or blocking of saline water;
second and third opening/closing valves 134 and 135 disposed at both ends of the biological sample supply pipe 133 and controlling the supply or blocking of the biological sample MB;
A fourth on/off valve 137 disposed at the outlet 136 and controlling the discharge of separated tissue cells (MB');
a memory 310 storing control programs for collection, separation, and quantitative injection of the biological sample (MB) according to the type of the biological sample (MB); and
According to the control program for collection, separation, and quantitative injection of the biological sample (MB) stored in the memory 310, the rotation speed of the rotation shaft 126 is adjusted to inject a quantitative amount of the biological sample (MB), ), the first to third on/off valves (124, 134, 135) are opened and the fourth on/off valves (137) are closed, and when separating and quantitatively injecting the biological sample (MB), the first to third on/off valves (124, 134, 135) are opened and closed. a control unit 440 that closes the valves 124, 134, and 135 and opens the fourth on/off valve 137;
A biological sample collection, separation and quantitative injection device comprising a.