KR102536424B1 - Non-surgical integrated biometric sample collection and separation and quantitative injection system - Google Patents

Abstract

The present invention relates to a non-surgical all-in-one biological sample collection separation and quantitative injection system, which extracts biological cells from a specific part of a patient, separates them into micro unit cells, and transfers the separated tissue-separated cells (MB') to the patient. The injected tissue-separated cells (MB′) can self-differentiate and proliferate to treat the affected area.
The non-surgical integrated biological sample collection separation and metering injection system according to the present invention inserts and fixes a needle 181 for collecting biological samples or a needle 171 for injection of tissue separation cells, collects and a cap 540 for fixing a cone-shaped needle having a germicidal lamp 400 for preventing infection for irradiating sterilizing light to the patient's collection and injection site during injection; The cap 540 for fixing the needle is fastened to one side, and a start processing button 410 for controlling collection, separation, and quantitative injection of the biological sample MB is disposed on the outer side, and the start processing button 410 Cylindrical shape having a tissue separation device 100 for a biological sample that collects and separates a biological sample (MB) and stores the separated tissue-separated cells (MB') to inject a metered amount into a patient's treatment site by lower body 530; A cylindrical intermediate body having one side fastened to the other side of the lower body 530 and having a saline motor 420 supplying saline solution to the tissue separation device 100 of the biological sample and a storage tank 430 therein. (520); And one side is fastened to the other side of the intermediate body 520, the separation lever 550 is fastened to the other side, the power button 360, and the biological sample according to the patient's treatment area and the type of the biological sample MB ( A function setting button 370 for setting collection, separation, and metering injection functions of MB) and collection, separation, and metering injection operations of the biological sample (MB) are displayed on the screen, and The internal temperature and pressure of the cylindrical chamber 110 of the tissue separation device 100 of the biological sample measured by the rotation speed of the rotating shaft 126 of the tissue separation device 100 from the biological sample and the temperature sensor 330 A display screen 380 that displays, in real time, the pressure applied to the porous plate 140 when the piston 128 of the cylindrical chamber 110 descends, measured by the sensor 340, and the biological data stored in the memory 310 Controlling the collection, separation, and quantitative injection of the biological sample (MB) by adjusting the number of revolutions of the rotating shaft 126 through the drive motor 350 according to a control program for collection, separation, and quantitative injection of the sample (MB) It includes; an upper body 510 having a control unit 440.

Description

Non-surgical integrated biometric sample collection and separation and quantitative injection system}

The present invention relates to a non-surgical all-in-one biological sample collection separation and quantitative injection system, and more particularly, to a tissue isolation cell (MB') by extracting a biological material (hereinafter referred to as 'MB') from a specific part of a patient. It relates to a non-surgical all-in-one biological sample collection separation and quantitative injection system that separates and injects separated tissue-separated cells (MB') into a treatment site of a patient in a metered amount for rapid and accurate treatment with optimal adherence.

BACKGROUND OF THE INVENTION Today, the degradation of biological tissues or materials such as human, animal and plant tissues is known for a variety of purposes and applications.

A biological material (MB) may be dissected to perform a medical examination, such as a biopsy, or may be used for research purposes or to confirm a diagnosis as part of a particular medical treatment. It can also be inspected to obtain data and information about the same sample and disassembled to obtain samples and samples to be subsequently analyzed in the laboratory.

In the prior art, a biological sample (MB) may be disintegrated for the purpose of isolating cells present in the same biological sample (MB), or for preparing tissue pieces or micrografts to be used in clinical and regenerative treatment. A micrograft is understood to be a set of cells in an extracellular matrix that is of subclinical size and is invisible to the unaided eye and visible under a microscope.

Figure 1 is a diagram showing a typical structure of a biological tissue before degradation, schematically showing a typical biological sample (MB) composed of biological tissue in an integrated form before being subjected to degradation.

As can be seen in FIG. 1 , a biological tissue or material (MB), in its intact and undigested form, has a number of cells, typically denoted as cells (CEL), each of which is illustrated by a number of parallel lines. It has its own nucleus (NU) and cytoplasm (CT) located within the extracellular matrix (MAT).

The biological sample MB is associated with cells CEL, within each extracellular matrix MAT. For example, growth factors further include substances and components composed of extracellular proteins and inorganic components. In particular, these growth factors and substances FC are indicators of the viability of cells, that is, their ability to grow and respond to external conditions, and are essential for cells CEL to exhibit their full properties and functions.

The prior art disaggregates a biological sample (MB) for histological examination or analysis of cell composites or to obtain samples of the same biological sample (MB). Systems and devices for disassembling such a biological sample (MB) are disclosed in the prior art.

Among them, International Application No. PCT/IB2015/059571 (hereinafter referred to as 'prior art literature') discloses a device for disassembling a biological sample, a corresponding manufacturing method, and a method for preparing cell suspension and tissue micrografts.

As shown in FIG. 2 , the apparatus for disassembling a biological sample includes an inner chamber 12 having an upper loading chamber 12a inside which loads a biological sample MB to be degraded, and an inner upper portion of the inner chamber 12. (11a) and a cylinder-shaped hollow outer body 11 for collecting the decomposed biological sample (MB) in the lower collection chamber 12b of the lower part 11b, and the inner chamber 12 below the transverse A porous plate or disintegration grid 13 that is housed in a direction, has fine holes 13a having sharp edges, and decomposes the biological sample MB through the fine holes 13a, and the inner chamber ( 12) The blade rotor 14 rotates in the upper loading chamber 12a to pass the biological sample MB contained in the upper loading chamber 12a through the fine holes 13a of the dissociation grid 13 so that the biological sample MB is decomposed It includes, and the micropores 13a of the porous decomposition grid 13 have a dimension or diameter D between 70 and 80 μm.

In the biological sample decomposition device of the prior art document, biological cells are extracted from a specific part of the patient with a syringe, collected on a cloth or cotton cloth, and then the skin tissue unnecessary to the biological cells is manually trimmed and removed using tweezers and a scalpel. , and loaded into the inside of the inner chamber 12. Thereafter, the cover 15 is closed, a motor is connected to the shaft 14a, and then the distribution blade 14b and the lower scraper 14c are rotated by rotating the biological sample MB. As the biological cells pass through the micropores 13a of the porous plate 13 by the rotation of the distribution blade 14b, the biological sample MB is pulverized and collected in the lower collection chamber 12b. Here, the lower scraper 14c rotates together with the shaft 14a to scrape the pulverized biological sample MB coming out of the fine hole 13a of the distribution blade 14b and collects it in the lower collection chamber 12b. do.

However, the biological sample decomposition device of the prior art document has the following problems.

1) Since biological cells are extracted from a specific area of the patient with a syringe and temporarily collected on a cotton cloth or cotton cloth, there is a high possibility of contamination with bacteria, and if the operation time is long, there is a problem that the living cells are damaged or deteriorated.

2) By inhaling the living cells temporarily collected on a cotton cloth or cotton cloth during the operation time again using a syringe and loading them inside the inner chamber 12, there is a high probability that the living cells will be contaminated with bacteria, and the procedure is time-consuming and cumbersome There is a problem.

3) The hair follicles separated from the scalp still have unnecessary skin tissue including the epidermal layer, the dermis layer, and the first fat layer attached around the hair follicles, so the hair follicle separator manually trims them using tweezers and a scalpel to remove unnecessary skin tissue from the hair follicles. Remove. At this time, it is inconvenient for the hair follicle separator to remove unnecessary skin tissue attached to the hair follicle while supplying saline from time to time to prevent the hair follicle from drying out, and to arrange the area around the hair follicle to be suitable for transplantation.

4) When the distribution blade 14b rotates at high speed, there is a problem in that a large amount of biological cells are destroyed or separated by the crushing blade of the blade.

5) There is a problem in that biological cells are destroyed or damaged due to frictional heat generated when the distribution blade 14b and the lower scraper 14c rotate at high speed.

6) Since the external air always penetrates into the inner chamber 12 when the cover 15 is opened or closed, there is a possibility that living cells may be contaminated by bacteria.

7) The biological sample decomposition device has a length (or size) of less than 4 to 5 cm, but has a complicated internal structure and a large number of parts, which increases manufacturing cost. For example, the biological sample disintegration device includes a cover 15, an inner chamber 12, a shaft 14a, a distribution blade 14b, a porous plate 13, a lower scraper 14c, and a lower collection chamber 12b. Since it includes a hollow outer body 11 having a structure, there is a problem in that the configuration is too complicated and the cost of replacement and repair of parts increases.

8) According to the biological sample MB, the distance and pressure between the distribution blade 14b rotated by the shaft 14a and the lower scraper 14c and the porous plate 13 should be adjusted, and the distribution blade 14b There is also a problem of adjusting the rotation speed of the .

9) Since the grinding blade of the distribution blade 14b becomes dull over time, there is a problem in that it must be replaced at regular intervals.

10) Since the biological sample (MB) is disassembled in the biological sample disintegrator and then the disassembled tissue-separated cells (MB') are injected into the patient's treatment area using a syringe, it is vulnerable to bacterial infection and the procedure is complicated. And there is a problem that the procedure takes a lot of time.

11) Since the biological sample disintegration device crushes the biological sample (MB) and then injects it into the patient's treatment site using a syringe, it is difficult to inject a fixed amount because the injected amount is different each time.

In addition, all of the biological sample decomposition devices currently used in Korea are imported products developed in foreign countries, and the product is expensive, and after service is difficult in case of failure.

Therefore, it is necessary to localize a product of a device or system that extracts, decomposes (or separates) and implants a biological sample (MB), and for this purpose, continuous research and development is required.

In addition, the medical device industry is a promising future industry that will continue to grow as long as mankind exists, and it is expected that the scale and scope will expand simultaneously with technological innovation, so domestic R&D is urgently needed to preoccupy technology.

International Application No. PCT/IB2015/059571 (International Application Date: 2015.12.14.) Domestic Patent Publication No. 10-2021-0078460 (Publication date: 2021.06.28.)

In order to solve the above problems, the technical problem to be achieved by the present invention is to extract biological cells from a specific area of a patient, separate them into tissue-separated cells (MB'), and use the separated tissue-separated cells (MB') to treat the patient. The purpose of the present invention is to present a non-surgical all-in-one biological sample collection, separation, and quantitative injection system in which a meter is injected into a site and the injected tissue-isolated cells (MB') self-differentiate and proliferate to treat the affected area.

In addition, another technical problem to be achieved by the present invention is to extract autologous hair follicle cells from the scalp with many progenitor cells behind the ears of patients with little hair loss, and then separate them into tissue-separated cells (MB') through a syringe. The purpose is to present a non-surgical all-in-one biological sample collection separation and quantitative injection system that is injected into the area in need of hair loss treatment and treated.

In addition, another technical problem to be achieved by the present invention is a non-surgical integrated type that prevents contamination or bacterial infection and prevents cell damage or deterioration by collecting, separating, and quantitatively injecting a biological sample (MB) in an enclosed space from the outside. The purpose is to present a biological sample collection separation and quantitative injection system.

In addition, another technical problem to be achieved by the present invention is to collect, separate, and quantitatively inject a biological sample (MB) in an external and closed space, thereby generating dermal papilla (Keratinocyte), progenitor cells ( The purpose is to present a non-surgical all-in-one biological sample collection separation and quantitative injection system that prevents stable separation and damage of precursor cells).

In addition, another technical problem to be achieved by the present invention is the collection of biological samples (MB), cell separation, and quantitative injection are performed quickly and accurately, and non-surgical integral biological sample collection with a simple procedure and greatly reduced procedure time compared to the conventional method. The purpose is to present a separate and metered injection system.

In addition, another technical problem to be achieved by the present invention is to collect, separate, and quantitatively inject biological samples (MB) with the pressure of a piston in an enclosed space from the outside, thereby preventing the problem of biological cells being destroyed or damaged by grinding blades. The purpose is to present a non-surgical all-in-one biological sample collection separation and quantitative injection system.

In addition, another technical problem to be achieved by the present invention is to provide a non-surgical integrated biological sample collection separation and quantitative injection system that has a simple configuration, is unlikely to fail, and can be used semi-permanently without replacing parts.

In addition, another technical problem to be achieved by the present invention is to present a non-surgical integrated biological sample collection, separation and quantitative injection system that separates cells and tissues of a biological sample (MB) according to the characteristics of the treatment area.

In addition, another technical problem to be achieved by the present invention is to provide a non-surgical integrated biological sample collection, separation and quantitative injection system that separates cells and tissues according to the type and state of a biological sample (MB).

In addition, another technical problem to be achieved by the present invention is to present a non-surgical integrated biological sample collection separation and quantitative injection system that presents an efficient and innovative treatment method without pain and scars using a non-surgical treatment method. .

In addition, another technical problem to be achieved by the present invention is to minimize infection and cell damage during transplantation of tissue isolation cells (MB') and propose a non-surgical all-in-one biological sample collection separation and quantitative injection system composed of a lightweight unit for optimal adherence. But it has a purpose.

In addition, another technical problem to be achieved by the present invention is to present a new concept of a non-surgical integrated biological sample collection separation and quantitative injection system using vacuum compression technology as a non-surgical treatment method.

In addition, another technical problem to be achieved by the present invention is to provide a non-surgical all-in-one biological sample collection, separation, and quantitative injection system in which the interior of the chamber is easily cleaned and parts are replaced.

The problems of the present invention are not limited to those mentioned above, and other problems not mentioned will be clearly understood by those skilled in the art from the description below.

As a means for solving the above-described technical problem, the non-surgical integrated biological sample collection, separation and quantitative injection system according to the present invention, as described in claim 1, includes a needle 181 for collecting biological samples or an injection for tissue isolation cell injection For fixing a cone-shaped needle having a sterilizing lamp 400 for preventing infection for inserting and fixing a needle 171 and irradiating sterilizing light to the collection and injection site of a patient when collecting and injecting a biological sample (MB) cap 540; The cap 540 for fixing the needle is fastened to one side, and a start processing button 410 for controlling collection, separation, and quantitative injection of the biological sample MB is disposed on the outer side, and the start processing button 410 Cylindrical shape having a tissue separation device 100 for a biological sample that collects and separates a biological sample (MB) and stores the separated tissue-separated cells (MB') to inject a metered amount into a patient's treatment site by lower body 530; A cylindrical intermediate body having one side fastened to the other side of the lower body 530 and having a saline motor 420 supplying saline solution to the tissue separation device 100 of the biological sample and a storage tank 430 therein. (520); And one side is fastened to the other side of the intermediate body 520, the separation lever 550 is fastened to the other side, the power button 360, and the biological sample according to the patient's treatment area and the type of the biological sample MB ( A function setting button 370 for setting collection, separation, and metering injection functions of MB) and collection, separation, and metering injection operations of the biological sample (MB) are displayed on the screen, and The internal temperature and pressure of the cylindrical chamber 110 of the tissue separation device 100 of the biological sample measured by the rotation speed of the rotating shaft 126 of the tissue separation device 100 from the biological sample and the temperature sensor 330 A display screen 380 displaying in real time the pressure applied to the porous plate 140 when the piston 128 of the cylindrical chamber 110 descends, measured by the sensor 340, and the biological information stored in the memory 310 Controlling the collection, separation, and quantitative injection of the biological sample (MB) by adjusting the number of revolutions of the rotating shaft 126 through the driving motor 350 according to a control program for collection, separation, and quantitative injection of the sample (MB) An upper body 510 having a control unit 440; characterized by a biological sample collection separation and quantitative injection system including.

As described in claim 2, the apparatus 100 for separating tissue from a biological sample has a cylindrical shape with a space formed therein and an open bottom, a flange 121 formed around the lower end, and a female screw fixing part in the center of the upper surface. An upper body 120 in which 125 is formed, coupled to the upper body 120, has a space formed therein, has a cylindrical shape with an open top, a flange 131 is formed around the upper end, and the inner lower surface is at the center A cylindrical chamber 110 having a lower body 130 formed inclined toward the discharge port 136 and having a lower body 130 protruding from the lower portion of the injection needle insertion portion 138 connected to the discharge port 136 therein; A piston 128 reciprocating in the upper body 120; Connected vertically to the piston 128, the male thread forming part 127 formed on the outside engages with the female thread of the female screw fixing part 125 and converts normal/reverse rotation into vertical reciprocating motion to move the piston 128. A rotating shaft 126 that raises and lowers; It is disposed between the flange 121 of the upper body 120 and the flange 131 of the lower body 130, and a plurality of fine holes 141 are formed vertically through the entire surface, so that the fine holes on the lower surface A porous plate 140 formed smaller than the micropores of the upper surface; a saline water supply pipe 122 formed inside the side surface of the upper body 120 and supplying saline water to the inner space of the upper body 120 through the saline water inlet 123 on the upper surface; and a biological sample supply pipe 133 formed inside the side surfaces of the upper and lower bodies 120 and 130 and injecting the biological sample MB into the inner space of the upper body 120 through the needle insertion part 138. It is characterized by a biological sample collection separation and quantitative injection system including ;

As described in claim 3, the apparatus 100 for separating tissue from a biological sample includes: a first opening/closing valve 124 disposed in the saline supply pipe 122 and controlling supply or blocking of saline; second and third opening/closing valves 134 and 135 disposed at both ends of the biological sample supply pipe 133 and controlling the supply or blocking of the biological sample MB; and a fourth opening/closing valve 137 disposed at the outlet 136 and controlling discharge of the separated tissue isolation cells (MB').

As described in claim 4, the biological sample collection separation and quantitative injection system includes: a power supply unit 300 supplying power from a rechargeable battery or commercial power; a memory 310 that stores control programs for collecting, separating, and quantitatively injecting the biological sample MB according to the treatment site and the type of the biological sample MB; a rotational speed measuring device 320 for measuring the rotational speed of the rotating shaft 126 in real time to detect the rising and falling speed of the piston 128; a temperature sensor 330 for measuring the internal temperature of the cylindrical chamber 110 in real time; a pressure sensor 340 which measures the pressure applied to the biological sample MB or the porous plate 140 in real time when the piston 128 descends; a driving motor 350 rotating the rotational shaft 126; and controlling the number of revolutions of the rotating shaft 126 through the drive motor 350 according to a control program for collection, separation, and metered injection of the biological sample MB stored in the memory 310 to obtain the biological sample MB. is quantitatively injected, and when the biological sample MB is collected, the first to third opening/closing valves 124, 134, 135 are opened and the fourth opening/closing valve 137 is closed, and the biological sample MB is separated and metered injection In the case of the first to third on-off valves 124, 134, 135 and the control unit 440 for opening the fourth on-off valve 137; characterized by a biological sample collection separation and metering injection system further comprising.

According to the present invention, as a non-surgical treatment method in which a patient's specific autologous cells are extracted, isolated, quantitatively injected, and the cells self-differentiate and proliferate to treat the affected area, there is no pain and scarring, and treatment is improved. Because the effect is large, it has the effect of providing efficient and innovative treatment to patients.

In addition, it can be treated with a single injection within a short time through autologous microtransplantation technology, and there is an economical effect of saving both cost and time as well as excessive stress on the patient himself.

In addition, it can be applied to various medical fields, including hair loss treatment, knee joint treatment, periodontal disease treatment, and skin burn treatment, and has an effect that can be applied to the aesthetic relief industry through body tissue microtransplantation.

In addition, it is possible to participate in the infrastructure business of peripheral medical equipment linked to the density of the suspension and the corresponding technology, and there is an effect that can contribute to the localization and overseas export of biofollicle sample manufacturing medical devices.

In addition, the supply of low-cost and high-efficiency medical devices can reduce personal and social costs for the treatment of alopecia, reduce stress caused by alopecia in individuals, and reduce damages derived from it.

In addition, high-quality medical services can be provided to consumers through the efficient supply of medical devices by medical institutions.

In addition, it is possible to operate at low cost and in a short time, away from the high cost and long time required for hair transplantation, and there is an effect that anyone can easily perform the procedure at a low cost and easily perform the procedure and recovery anytime, anywhere.

The effects of the present invention are not limited to those mentioned above, and other effects not mentioned will be clearly understood by those skilled in the art from the description below.

1 is a diagram showing a typical structure of a biological tissue before decomposition.
2 is an exploded perspective view of an apparatus for decomposing a biological sample disclosed in Korean Patent Publication No. 10-2017-0102479 as a prior art document.
3 to 6 are diagrams showing a non-surgical integrated biological sample collection separation and quantitative injection system according to an embodiment of the present invention,
3 is a perspective view of a fixed shape of an injection needle 181 for collecting biological samples;
4 is a perspective view of a fixed shape of an injection needle 171 for injecting tissue-isolated cells;
5 is an exploded perspective view of a biological sample collection separation and quantitative injection system 500;
6 is a block configuration diagram of a biological sample collection, separation, and quantitative injection system 500 .
7 to 14 are views showing the tissue separation device 100 for biological samples installed inside the biological sample collection, separation and metering injection system 500,
7 is a perspective view,
8 is an exploded perspective view,
9 is a cross-sectional view;
10 is a cross-sectional view of a state in which an injection needle 181 for collecting biological samples is inserted.
11 is a cross-sectional view of a state in which an injection needle 171 for injecting tissue-isolated cells is inserted;
12 is a perspective view of porous plate 140;
13 is a view showing a conical microhole 200,
14 is a view showing star-shaped horn-shaped micropores 200.
15 is a view showing a cell suspension obtained by the tissue separation device for a biological sample according to the present invention.

Hereinafter, embodiments of the present invention will be described in detail so that those skilled in the art can easily practice with reference to the accompanying drawings. However, the present invention may be implemented in many different forms and is not limited to the embodiments described herein. In addition, in order to clearly explain the present invention in the drawings, parts irrelevant to the description are omitted, and similar reference numerals will be attached to similar parts throughout the description of the invention.

Hereinafter, with reference to the accompanying drawings for the specific technical content to be carried out in the present invention will be described in detail.

Embodiment of biological sample collection separation and metering system 500

3 to 6 are views showing a non-surgical integrated biological sample collection separation and quantitative injection system according to an embodiment of the present invention. FIG. 3 is a perspective view of a fixed shape of a needle 181 for collecting biological samples, is a perspective view of a fixed shape of the injection needle 171 for tissue isolation cell injection, FIG. 5 is an exploded perspective view of the biological sample collection, separation and metering injection system 500, and FIG. 6 is a biological sample collection, separation and metering injection system ( 500) is a block configuration diagram.

First, the non-surgical integrated biological sample collection separation and quantitative injection system 100 according to the present invention is suitable and advantageous for the preparation of cell suspensions and the preparation of tissue micrografts, and is suitable for tissue isolation cells (MB'). ) is a cell suspension or set of cells of subclinical size, i.e., a matrix of cells invisible to the unaided eye but visible under a microscope.

A biological sample (MB) is a typical biological tissue composed of biological tissue in an integrated form before being affected by a decomposition operation, and has a structure as shown in FIG. 1 .

As can be seen in FIG. 1 , a biological tissue or material, in its intact and undegraded form, has a plurality of cells, typically denoted as cells (CEL), each of which is an extracellular matrix depicted by a number of parallel lines. It has its own nucleus (NU) and cytoplasm (CT) located within the (MAT).

The biological sample MB is associated with cells CEL, within each extracellular matrix MAT. For example, growth factors further include substances and components composed of extracellular proteins and inorganic components. In particular, these growth factors and substances FC are indicators of the viability of cells, that is, their ability to grow and respond to external conditions, and are essential for cells CEL to exhibit their full properties and functions.

For reference, FIG. 15 is a view showing a cell suspension obtained by the tissue separation device of a biological sample according to the present invention, and its own nucleus (NU), cytoplasm (CT) and growth factors located in the extracellular matrix (MAT) It contains substances (FC).

As shown in FIGS. 3 to 6, the non-surgical integrated biological sample collection separation and metering injection system 500 according to the present invention largely consists of an upper body 510, a middle body 520, a lower body 530, and needle fixation. It is separated by a cap 540.

The fixing cap 540 has a conical shape, and the female screw part 541 formed on the inside of the other side is fastened and fixed to the male screw part 532 formed on one side of the lower body 530. The fixing cap 540 is fixed by inserting an injection needle 181 for collecting a biological sample or an injection needle 171 for injecting tissue-isolated cells through a hole through the center thereof.

The fixing cap 540 is formed of a transparent or translucent light-transmitting material, and a germicidal lamp 400 for preventing infection is disposed therein. The infection-preventing sterilization lamp 400 serves to prevent infection and sterilization by radiating sterilizing light to the collection and injection site of the patient when the biological sample MB is collected and injected.

The lower body 530 has a cylindrical shape, and the cap 540 for fixing the needle is fastened to one side. Male screw portions 531 and 532 are formed on one side and the other side of the lower body 530 . However, either one or both of them may be formed as female screw parts according to the screw shape formed on the needle fixing cap 540 or the intermediate body 520 .

A start processing button 410 is disposed on the outer surface of the lower body 530 to control collection, separation, and quantitative injection of the biological sample MB. In addition, the inside of the lower body 530 collects and separates the biological sample (MB) by the start processing button 410, stores the separated tissue-separated cells (MB'), and injects them into the treatment area of the patient in a metered amount. It contains a tissue separation device 100 of a biological sample to do so.

Here, the configuration and operation of the biological sample tissue separation device 100 will be described in detail with reference to FIGS. 7 to 14 to be described later.

Subsequently, the intermediate body 520 has a cylindrical shape, and one side is fastened to the other side of the lower body 530 . Female screw parts 521 are formed on one side and the other side of the intermediate body 520 . However, one or both of the lower body 530 and the upper body 510 may be formed as male screw parts depending on the screw shape formed thereon.

The intermediate body 520 may have a saline motor 420 and a saline storage tank 430 therein. The saline motor 420 and the saline storage tank 430 serve to supply saline to the tissue separation device 100 for the biological sample.

The upper body 510 has a cylindrical shape, one side is fastened to the other side of the intermediate body 520, and the separation lever 550 is fastened to the other side. A male screw portion 511 is formed on one side of the upper body 510 . However, when the male screw part is formed on the other side of the intermediate body 520, it may be formed as a female screw part.

A power button 360, a function setting button 370, a display screen 380, and an operation indicator lamp 390 may be disposed on the outer surface of the upper body 510.

Here, the function setting button 370 sets functions for collection, separation, and quantitative injection of the biological sample MB according to the treatment area of the patient and the type of the biological sample MB.

The display screen 380 displays the collecting, separating, and quantitatively injecting operations of the biological sample MB on the screen, and the rotating shaft 126 of the tissue separation device 100 for the biological sample measured by the rotation speed measuring device 320. ) and the internal temperature of the cylindrical chamber 110 of the tissue separation device 100 for the biological sample measured by the temperature detector 330 and the cylindrical chamber 110 measured by the pressure detector 340 The pressure applied to the porous plate 140 when the piston 128 of the descends is displayed in real time. The operation indicator lamp 390 indicates the operation of collecting and separating and injecting the biological sample MB by lighting and flickering of the lamp. The display screen 380 may be configured to display the count of separated tissue-separated cells (MB').

In addition, the upper body 510 may include a power supply unit 300, a memory 310, and a control unit 440 therein.

Here, the power supply unit 300 supplies power from a rechargeable battery or commercial power. The memory 310 stores control programs for collection, separation, and quantitative injection of the biological sample MB according to the treatment site and the type of the biological sample MB. At this time, the control program controls the rotational speed of the rotating shaft 126 of the tissue separation device 100 for the biological sample, the internal temperature and pressure of the cylindrical chamber 110, etc. according to the treatment site and the type of the biological sample MB. is programmed to automatically adjust.

The control unit 440 automatically controls the operation of the tissue separation device 100 for the biological sample according to a control program for collecting, separating, and quantitatively injecting the biological sample MB stored in the memory 310 . For example, the number of rotations of the rotating shaft 126 of the biological sample tissue separation device 100 is adjusted, and the number of rotations of the rotating shaft 126 and the operation of the first to fourth opening/closing valves 124, 134, 135, and 137 are controlled. It controls the collection, separation and quantitative injection of the biological sample (MB).

For example, the control unit 420 opens the first to third opening/closing valves 124, 134, and 135 and closes the fourth opening/closing valve 137 when collecting the biological sample MB, and In case of separation and quantitative injection, the first to third opening/closing valves 124, 134, 135 are closed, the fourth opening/closing valve 137 is opened, and the number of revolutions of the rotating shaft 126 is adjusted to obtain the biological sample MB. Control dosing.

In addition, the biological sample collection separation and metering injection system 500 may include a rotation rate measuring device 320 , a temperature sensor 330 , a pressure sensor 340 , and a driving motor 350 .

The rotational speed measuring device 320 measures the rotational speed of the rotational shaft 126 in real time to detect the rising and falling speed of the piston 128 inside the cylindrical chamber 110 of the tissue separation device 100 for the biological sample. will be measured with The rotation speed measuring device 320 may be configured in the cylindrical chamber 110 .

The temperature sensor 330 serves to measure the internal temperature of the cylindrical chamber 110 in real time. In addition, the pressure sensor 340 serves to measure the pressure applied to the biological sample MB or the porous plate 140 in real time when the piston 128 descends. The temperature sensor 330 and the pressure sensor 340 may be configured inside the cylindrical chamber 110 .

The driving motor 350 is a driving source for rotating the rotating shaft 126 and may be disposed on the lower body 530 together with the tissue separation device 100 for the biological sample.

Hereinafter, the apparatus 100 for separating tissue from a biological sample will be described with reference to the accompanying drawings.

Embodiment of apparatus 100 for tissue separation of biological samples

7 to 14 are views showing the tissue separation device 100 for a biological sample installed inside the biological sample collection, separation and metering injection system 500, where FIG. 7 is a perspective view and FIG. 8 is an exploded perspective view. 9 is a cross-sectional view, FIG. 10 is a cross-sectional view of a state in which an injection needle 181 for collecting biological samples is inserted, FIG. 11 is a cross-sectional view of a state in which an injection needle 171 for tissue isolation cell injection is inserted, and FIG. 12 is a porous It is a perspective view of the plate 140, FIG. 13 is a view showing the conical microholes 200, and FIG. 14 is a view showing the star-shaped cone-type microholes 200. And FIG. 15 is a view showing a cell suspension obtained by the tissue separation device for a biological sample according to the present invention.

As shown in FIGS. 7 to 11 , the apparatus 100 for separating tissue from a biological sample according to the present invention includes a cylindrical chamber 110 largely composed of an upper body 120 and a lower body 130 .

The upper body 120 has a cylindrical shape with a space formed therein and an open bottom, a flange 121 is formed around the lower end, and a female screw fixing part 125 is disposed or formed at the center of the upper surface. A biological sample (MB) to be decomposed is loaded in the inner space of the upper body 120 .

The lower body 130 is coupled to the upper body 120, has a cylindrical shape with a space formed therein and an open top, a flange 131 is formed around the upper end, and the inner lower surface has a central outlet ( 136) is formed inclined toward. An injection needle insertion part 138 protrudes from the lower part of the lower body 130, and the outlet 136 penetrates and is connected therein. In the inner space of the lower body 130, the tissue isolation cells MB' separated from the biological sample MB through the micropores 141 of the porous plate 140 are collected and loaded.

The upper and lower bodies 120 and 130 have ventilation holes 139 formed in side walls so that air can flow to each other, and a ventilation hole 129 is formed through the upper part of the upper body 120 as well. Due to this, the piston 128 can be smoothly raised and lowered inside the cylindrical chamber 110 .

The ventilation hole 139 formed in the lower body 130 is located under the porous plate 140 and may be formed as a micro hole. In addition, a mesh or a filter may be installed on the inner wall of the lower body 130 where the ventilation hole 139 is formed so that the tissue isolation cells MB' are not discharged to the outside through the ventilation hole 139 .

Inside the upper body 120, the piston 128 reciprocates while vertically rising and falling. The piston 128 has a disk shape and a rotating shaft 126 is vertically connected to the upper portion. The rotating shaft 126 is installed to rotate through the female screw fixing part 125 disposed at the upper center of the upper body 120 and the upper body 120 . At this time, the rotational shaft 126 is installed so that the male thread forming part 127 formed by a certain length on the outer side of the rotating shaft 126 engages with the female thread of the female screw fixing part 125. Accordingly, when the rotating shaft 126 rotates forward and backward, the male thread forming part 127 engages with the female thread of the female screw fixing part 125 and converts it into a vertical reciprocating motion to raise and lower the piston 128. .

The upper body 120 has a saline supply pipe or a saline supply passage 122 formed inside the side surface. The saline supply pipe or the saline supply passage 122 has one side connected to the saline inlet 123 formed on the upper surface of the upper body 120 and the other side connected to the inner space of the upper body 120. In addition, a first opening/closing valve 124 is disposed on one side or the other side of the saline water supply pipe or the saline water supply passage 122, and the upper body ( 120) to supply and block the saline solution to the inner space.

A porous plate 140 may be disposed between the flange 121 of the upper body 120 and the flange 131 of the lower body 130 . Here, the porous plate 140 has a plurality of fine holes 141 vertically penetrating the entire surface of the disc shape, and the size of the fine holes decreases from the upper surface to the lower surface.

Here, the fine holes 141 may be formed to have a dimension or diameter between 60 and 80 μm. Preferably, it may be formed to have a dimension or diameter of 75 μm or less. Further, the shape of the microholes 141 may be formed in a conical, triangular pyramidal, quadrangular pyramidal, pentagonal pyramidal, hexagonal pyramidal, and star pyramidal micropores.

Referring to FIG. 13, the conical microholes 200 have a circular inlet 201 formed on the upper surface of the porous plate 140 and a sharp edge-shaped outlet 202 formed on the lower surface thereof, and the inlet 201 ) than the outlet 202 is formed smaller.

Referring to FIG. 14, the star cone-shaped microhole 240 has a circular inlet 241 formed on the upper surface of the porous plate 140 and a sharp edge-shaped outlet 242 formed on the lower surface thereof, and the inlet ( 241, the outlet 242 is formed smaller.

The biological sample MB passes through the micropores 141 of the porous plate 140 and is separated into tissue-isolated cells MB′ having a size of 20 to 75 μm or less. For reference, FIG. 14 shows a cell suspension of the tissue-isolated cells (MB').

The material of the porous plate 140 may be made of stainless steel, metal or alloy, and may be formed using a mold, a die-punch, or the like.

O-rings 150 and 151 may be disposed between the flange 121 of the upper body 120 and the porous plate 140 and between the porous plate 140 and the flange 131 of the lower body 130. The O-rings 150 and 151 may be formed of circular rings, natural rubber, synthetic rubber, synthetic resin, or the like, and serve as an air seal.

Subsequently, a biological sample supply pipe or a biological sample supply passage 133 is formed inside the side surfaces of the upper and lower bodies 120 and 130 . The biological sample supply pipe or biological sample supply passage 133 has one side connected to the inner space of the upper body 120 and the other side connected to the outlet 136 of the lower body 130 .

Second and third opening/closing valves 134 and 135 are disposed on one side or the other side of the biological sample supply pipe or the biological sample supply passage 133, and the second and third opening/closing valves 134 and 135 are opened and closed to collect biological samples. The biological sample MB is collected through the injection needle 181 and injected into the inner space of the upper body 120 . At this time, the biological sample MB injected into the inner space of the upper body 120 is loaded on the top of the porous plate 140 .

The second and third opening/closing valves 134 and 135 are disposed at both ends of the biological sample supply pipe 133 and control the supply or blocking of the biological sample MB into the inner space of the upper body 120 .

A fourth opening/closing valve 137 is disposed at the outlet 136 of the lower body 130. The fourth opening/closing valve 137 controls the discharging of the tissue-isolated cells (MB′) separated while passing through the micropores 141 of the porous plate 140 through the outlet 136 .

An apparatus for separating tissue from a biological sample according to the present invention extracts biological cells from a specific area of a patient, separates them into tissue-separating cells (MB'), and injects the separated tissue-separating cells (MB') into the patient's treatment area in a fixed amount. , the injected tissue-separated cells (MB') can self-differentiate and proliferate to treat the affected area.

15 is a view showing a cell suspension obtained by the tissue separation apparatus of a biological sample according to the present invention, and its own nucleus (NU) and cytoplasm (CT) and growth factors and substances located in the extracellular matrix (MAT) ( FC) included.

Example of a method for separating tissue from a biological sample

A method for isolating tissue from a biological sample according to the present invention is described as follows.

First, the injection needle 181 for collecting a biological sample is fixed to the injection needle insertion part 138 of the cylindrical chamber 110 .

Then, after anesthetizing the patient's biological sample collecting part, the biological sample collecting injection needle 181 is inserted into the patient's body, and then the piston 128 of the cylindrical chamber 110 is raised and sucked. The biological sample (MB) is collected through the needle 181 for collecting the biological sample and loaded on the top of the porous plate 140 inside the upper body 120 .

Then, saline is supplied to the biological sample MB loaded on the porous plate 140 through the saline supply pipe 122 .

Then, the first to fourth opening/closing valves 124, 134, 135, and 137 of the cylindrical chamber 110 are all closed, and pressure is applied while the piston 128 is lowered so that the biological sample MB moves to the porous plate 140. ) to pass through the fine holes 141. At this time, the upper and lower bodies 120 and 130 have vents 139 formed inside the side walls so that air can flow to each other, and vents 129 are formed through the upper part of the upper body 120 as well. Due to this, the piston 128 can be smoothly raised and lowered inside the cylindrical chamber 110 .

The biological sample (MB) (see FIG. 1 ) passes through the micropores 141 of the porous plate 140 and is separated into tissue-separated cells (MB') (see FIG. 15 ), and then inside the lower body 130 . are loaded into

Next, the injection needle 171 for tissue isolation cell injection is inserted into the injection needle insertion part 138 of the cylindrical chamber 110 and fixed thereto. After closing the first to third opening/closing valves 124, 134, and 135 and opening the fourth opening/closing valve 137, the tissue isolation cell injection needle 171 is injected into the patient's treatment area to separate the tissue. The cells (MB') are dose-injected. At this time, the quantitative injection of the tissue-separated cells (MB') is performed by adjusting the number of revolutions of the rotating shaft 126 so that the constant quantitative injection is performed.

As described above, the non-surgical integrated biological sample collection, separation and quantitative injection system according to the present invention extracts biological cells from a specific part of a patient, separates them into tissue-separated cells (MB'), and separates tissue-separated cells (MB'). ) is dosed into the patient's treatment site, and the injected tissue-separated cells (MB') self-differentiate and proliferate to treat the affected area, thereby solving the technical problem of the present invention.

Preferred embodiments of the present invention described above have been disclosed to solve the technical problems, and those skilled in the art (those skilled in the art) to which the present invention belongs can make various modifications, changes, additions, etc. within the spirit and scope of the present invention. This will be possible, and such modifications and changes should be regarded as belonging to the scope of the following claims.

The non-surgical integrated biological sample collection, separation and quantitative injection system according to the present invention can be applied to various medical fields, including hair loss patients, arthritis patients, periodontal disease patients, and burn patients. In addition, it can be applied to the aesthetic alleviation industry through body tissue microtransplantation. In addition, it can be applied to artificial head, artificial bone, ophthalmic prosthesis, dental implant, maxillary bone reconstruction, degenerative knee joint, otolaryngology, etc.

100: tissue separation device for biological samples
110: cylindrical chamber 120: upper body
121: flange
122: saline supply pipe or saline supply passage
123: saline inlet 124: first on-off valve
125: female screw fixing part 126: rotation shaft
127: Male thread forming part 128: Piston
129: vent 130: lower body
131: flange 132: screw hole
133: biological sample supply pipe or biological sample supply passage
134, 135: second and third opening and closing valves 136: outlet
137: fourth open/close valve 138: injection needle insertion part
139: vent 140: porous plate
141: fine hole 142: screw insertion hole
150, 151: O-ring 160: bolt
161: nut 170: syringe body
171: injection needle for tissue isolation cell injection
180: syringe body
181: injection needle for biological sample collection
190: extraction and injection separation pipe
200: conical microhole 201: inlet
202: pointed edge type outlet 210: triangular pyramid type fine hole
211: inlet 212: pointed edge outlet
220: quadrangular pyramidal microhole 221: inlet
222: pointed edge type outlet 230: pentagonal pyramid type fine hole
231: inlet 232: pointed edge outlet
240: star-shaped horn-shaped fine hole 241: inlet
242: pointed edge type outlet 300: power unit
310: memory 320: rotation speed meter
330: temperature sensor 340: pressure sensor
350: drive motor 360: power button
370: function setting button 380: display screen
390: Operation indicator lamp 400: Sterilization lamp for preventing infection
410: Start Processing Button
420: saline motor 430: saline storage tank
440: control unit
500: Non-surgical all-in-one biological sample collection separation and quantitative injection system
510: upper body 511: male thread
520: intermediate body 521: female thread
530: lower body 531, 532: male thread
540: Cap for fixing needle 541: Female screw part
550: Separator
MB: temperament as an indicator of viability
MB': biological sample disaggregated in cell suspension (MB)
CEL: Tissue isolated cells (MB')

Claims (4)

In the non-surgical integrated biological sample collection separation and quantitative injection system,
To prevent infection by inserting and fixing the injection needle 181 for collecting biological samples or the injection needle 171 for tissue separation cell injection, and irradiating sterilizing light to the collection and injection site of the patient when collecting and injecting the biological sample (MB) A cap 540 for fixing a cone-shaped needle having a germicidal lamp 400 therein;
The cap 540 for fixing the needle is fastened to one side, and a start processing button 410 for controlling collection, separation, and quantitative injection of the biological sample MB is disposed on the outer side, and the start processing button 410 Cylindrical shape having a tissue separation device 100 for a biological sample that collects and separates a biological sample (MB) and stores the separated tissue-separated cells (MB') to inject a metered amount into a patient's treatment site by lower body 530;
A cylindrical intermediate body having one side fastened to the other side of the lower body 530 and having a saline motor 420 supplying saline solution to the tissue separation device 100 of the biological sample and a storage tank 430 therein. (520); and
One side is fastened to the other side of the intermediate body 520, the separation lever 550 is fastened to the other side, the power button 360, and the biological sample MB according to the patient's treatment area and the type of the biological sample MB. ) function setting button 370 for setting collection, separation, and metering injection functions, and the collection, separation, and metering injection operation of the biological sample (MB) are displayed on the screen, and the rotation speed meter 320 measures the The number of revolutions of the rotating shaft 126 of the tissue separation device 100 for a biological sample, the internal temperature of the cylindrical chamber 110 of the tissue separation device 100 for a biological sample measured by the temperature sensor 330, and the pressure sensor A display screen 380 displaying in real time the pressure applied to the porous plate 140 when the piston 128 of the cylindrical chamber 110 descends, measured at 340, and a biological sample stored in the memory 310 The control unit controls the collection, separation, and quantitative injection of the biological sample (MB) by adjusting the number of revolutions of the rotating shaft 126 through the drive motor 350 according to the control program for collection, separation, and quantitative injection of MB. upper body 510 with 440;
Biological sample collection separation and quantitative injection system comprising a.
The method of claim 1,
The tissue separation device 100 of the biological sample,
An upper body 120 having a cylindrical shape with a space formed therein and an open bottom, a flange 121 formed around the lower end, and a female screw fixing part 125 formed in the center of the upper surface, and the upper body 120 It is coupled, a space is formed inside, it has a cylindrical shape with an open top, a flange 131 is formed around the top, the inner lower surface is formed inclined toward the central outlet 136, and the outlet 136 is connected to the inside. A cylindrical chamber 110 having a lower body 130 in which a needle insertion part 138 protrudes from a lower portion;
A piston 128 reciprocating in the upper body 120;
Connected vertically to the piston 128, the male thread forming part 127 formed on the outside engages with the female thread of the female screw fixing part 125 and converts normal/reverse rotation into vertical reciprocating motion to move the piston 128. A rotating shaft 126 that raises and lowers;
It is disposed between the flange 121 of the upper body 120 and the flange 131 of the lower body 130, and a plurality of fine holes 141 are formed vertically through the entire surface, so that the fine holes on the lower surface A porous plate 140 formed smaller than the micropores of the upper surface;
a saline water supply pipe 122 formed inside the side surface of the upper body 120 and supplying saline water to the inner space of the upper body 120 through the saline water inlet 123 on the upper surface; and
A biological sample supply pipe 133 formed inside the side surfaces of the upper and lower bodies 120 and 130 and injecting a biological sample MB into the inner space of the upper body 120 through the needle insertion part 138;
Biological sample collection separation and quantitative injection system comprising a.
The method of claim 2,
The tissue separation device 100 of the biological sample,
a first opening/closing valve 124 disposed in the saline water supply pipe 122 and controlling the supply or blocking of the saline water;
second and third opening/closing valves 134 and 135 disposed at both ends of the biological sample supply pipe 133 and controlling the supply or blocking of the biological sample MB; and
a fourth opening/closing valve 137 disposed at the outlet 136 and controlling discharge of the separated tissue isolation cells MB';
Biological sample collection separation and quantitative injection system further comprising a.
The method of claim 3,
The biological sample collection separation and quantitative injection system,
A power supply unit 300 supplying power from a rechargeable battery or commercial power;
a memory 310 that stores control programs for collecting, separating, and quantitatively injecting the biological sample MB according to the treatment site and the type of the biological sample MB;
a rotational speed measuring device 320 for measuring the rotational speed of the rotating shaft 126 in real time to detect the rising and falling speed of the piston 128;
a temperature sensor 330 for measuring the internal temperature of the cylindrical chamber 110 in real time;
a pressure sensor 340 which measures the pressure applied to the biological sample MB or the porous plate 140 in real time when the piston 128 descends;
a driving motor 350 rotating the rotational shaft 126; and
The biological sample MB is obtained by controlling the number of revolutions of the rotating shaft 126 through the driving motor 350 according to a control program for collecting, separating, and quantitatively injecting the biological sample MB stored in the memory 310 . When performing quantitative injection and collecting the biological sample MB, the first to third opening/closing valves 124, 134, 135 are opened and the fourth opening/closing valve 137 is closed, and the biological sample MB is separated and quantitatively injected. A control unit 440 for closing the first to third opening/closing valves 124,134,135 and opening the fourth opening/closing valve 137;
Biological sample collection separation and quantitative injection system further comprising a.

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