TW201427595A - The invention is described the use of chlorine dioxide solution to prevent or cure brown root rot disease of plants - Google Patents

The invention is described the use of chlorine dioxide solution to prevent or cure brown root rot disease of plants Download PDF

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TW201427595A
TW201427595A TW103100473A TW103100473A TW201427595A TW 201427595 A TW201427595 A TW 201427595A TW 103100473 A TW103100473 A TW 103100473A TW 103100473 A TW103100473 A TW 103100473A TW 201427595 A TW201427595 A TW 201427595A
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trees
chlorine dioxide
brown root
aqueous solution
brown
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TW103100473A
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Chinese (zh)
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Hao-Chang Yin
Shan-Shue Wang
Bin-Syuan Huang
Chu-Wei Hsu
Cheng-Je Wang
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Hao-Chang Yin
Chen Hsin Yuan
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Abstract

The invention is described the use of chlorine dioxide solution to prevent or cure brown root rot disease of plants. The composition of chlorine dioxide solution is made by chlorine dioxide gas dissolving in water and is the active ingredients to effectively eliminate or prevent brown root rot bacteria infecting to plants.

Description

一種防除樹木褐根病之組成物及方法 Composition and method for controlling brown root disease of trees

本發明係有關一種防除樹木褐根病之組成物及方法,特別是一種以二氧化氯水溶液組成物為有效成份以治療防除樹木、植栽褐根病菌之組成物及其治療、預防之方法。 The invention relates to a composition and a method for controlling brown root disease of trees, in particular to a composition for treating and controlling trees and planting brown root pathogens with the composition of chlorine dioxide aqueous solution as an active ingredient, and a method for treating and preventing the same.

樹木褐根病是位處熱帶、亞熱帶型態之亞洲、澳洲及非洲地區林木、果樹與特用作用重要的根部病害;該樹木褐根病係由褐根病菌(有害木層孔菌)(Phellinus noxius(Corner)Cunningham)寄生於樹木之根部,使樹木根部腐敗而導致全株萎凋死亡,尤其,由於褐根病菌在自然界鮮少形成子實體而不易發現,因此當罹患褐根病菌之樹木根部及莖基部出現茶褐色或黑褐色之菌絲面、或木材切面具有黃褐色網紋帶時,則該罹病樹木已受褐根病菌嚴重侵害而全株萎凋者。 Tree brown root disease is an important root disease in forests, fruit trees and special effects in tropical, subtropical Asia, Australia and Africa; the root browning disease is caused by brown root pathogen (Pseudoporus sinensis) (Phellinus) Noxius (Corner) Cunningham) is parasitic on the roots of trees, causing the roots of trees to become corrupt and causing the whole plant to wither and die. In particular, since the brown root pathogens rarely form fruit bodies in nature and are not easily found, they are roots of trees rooted in brown root pathogens. When the base of the stem has a brown or dark brown mycelium surface, or the wood cut surface has a yellow-brown mesh belt, the ricket tree has been seriously infested by the brown root pathogen and the whole plant has withered.

而當樹木出現褐根病之病徵,根部已有60~70%以上受害,且罹患褐根病末期的植物無法投藥救治,僅能將罹病樹木及根系掘出焚毀,並不能讓罹患褐根病菌之樹木得繼續存活,而無法保存具紀念意義或百年歷史之老樹;另一方面,病區內之建康植株也需進行預防工作,以避免殘留於土壤中之罹病根系、病菌再次傳染寄生至重植之樹木,而該預防處理的方法包含有掘溝阻斷法、燻蒸劑處理病土法、淹水處理法、藥劑混 土覆蓋法或稀釋灌注法…等;上述防治方法需耗費大量之人力、動用大型機械與藥劑來處理,且容易受限於地形因素而影響處理方法及成效而無法確實消滅褐根病菌者。 When the tree has the symptoms of brown root disease, more than 60~70% of the roots are affected, and the plants with brown root disease can not be treated. Only the rickets and roots can be excavated and burned, and the brown root disease can not be caused. The trees have to survive and cannot preserve the commemorative or centuries-old trees. On the other hand, the Jiankang plants in the ward also need to be prevented to avoid the roots and pathogens remaining in the soil. To replanted trees, and the method of prevention and treatment includes a trenching blocking method, a fumigant treatment method soil method, a flooding treatment method, and a chemical mixing method. The soil cover method or the dilution infusion method, etc.; the above-mentioned control method requires a large amount of manpower, uses large machinery and chemicals to treat, and is easily limited by topographical factors and affects the treatment method and effect, and cannot effectively eliminate the brown root pathogen.

本發明之主要目的,即在於提供一種可作為有效治療樹木、植栽褐根病之二氧化氯水溶液組成物,藉由該二氧化氯水溶液組成物殺滅防除寄生於樹木根部與植栽上之褐根病菌,使樹木、植栽不致因此褐化、萎凋死亡而可存活,且不受二氧化氯水溶液組成物之化學作用而影響其生長者。 The main object of the present invention is to provide a chlorine dioxide aqueous solution composition which can effectively treat trees and plant brown root disease, and the composition of the chlorine dioxide aqueous solution kills and controls parasitic roots and plants. The brown root pathogen can make trees and plants not survive due to browning and withering death, and is not affected by the chemical action of the chlorine dioxide aqueous solution composition.

本發明之另一目的,即在於提供一種樹木、植栽褐根病之防除方法,其特徵為以二氧化氯水溶液組成物處理感染褐根病菌之樹木、植栽,以有效防除殺滅寄生於樹木根部、植栽上之褐根病菌。 Another object of the present invention is to provide a method for controlling a tree and planting brown root disease, which is characterized in that a tree of chlorine-infected bacteria is treated with a chlorine dioxide aqueous solution composition, and the plant is planted to effectively prevent and kill parasitism. Roots of trees, brown root pathogens on plants.

本發明之又一目的,即在於提供一種保護樹木褐根病之方法,其特徵為以二氧化氯水溶液組成物預先處理樹木、植栽,以防除褐根病菌寄生於樹木根部與植栽上,達到有效預防之效果。 Another object of the present invention is to provide a method for protecting brown root disease of trees, which is characterized in that trees and plants are pre-treated with a composition of an aqueous solution of chlorine dioxide to prevent parasitic bacteria from parasitizing on roots and plants of trees. Achieve effective prevention.

為達到上述目的,本發明之二氧化氯水溶液組成物,係將二氧化氯氣體溶於水中使形成二氧化氯水溶液組成物,利用該二氧化氯水溶液組成物處理感染褐根病菌之樹木、植栽,藉以防除殺滅寄生於樹木根部與植栽之褐根病菌,使感染之樹木、植栽可存活而不致萎凋死亡,且可處理未感染褐根病菌之樹木、植栽,以達有效防除褐根病菌。 In order to achieve the above object, the chlorine dioxide aqueous solution composition of the present invention dissolves the chlorine dioxide gas in water to form a chlorine dioxide aqueous solution composition, and the chlorine dioxide aqueous solution composition is used to treat the tree infected with the brown root pathogen. Planting, in order to prevent the killing of the roots of the trees and the planting of the brown root pathogens, so that the infected trees and plants can survive without causing death, and can treat trees and plants that are not infected with brown root pathogens, so as to effectively prevent them. Brown root pathogen.

有關本發明防除樹木、植栽褐根病之處理方法,並無特別限制,例如樹體注入處理、樹冠下土壤處理、莖葉處理、塗布處理…等以二 氧化氯水溶液組成物處理樹木方法,皆可使用。 The method for treating tree and planting brown root disease according to the present invention is not particularly limited, and is, for example, a tree infusion treatment, a soil treatment under a canopy, a stem and leaf treatment, a coating treatment, etc. The method of treating the trees with the oxidized chlorine aqueous solution composition can be used.

有關本發明保護樹木、植栽感染褐根病之處理方法,並無特別限制,例如樹體注入處理、樹冠下土壤處理、莖葉處理、塗布處理…等以二氧化氯水溶液組成物方法,皆可使用。 The method for treating the tree and planting brown root disease of the present invention is not particularly limited, and for example, a tree body injection treatment, a soil treatment under the canopy, a stem and leaf treatment, a coating treatment, etc. be usable.

第1圖係本發明未浸置二氧化氯水溶液組成物之控制組木質部切塊其10天後菌絲生長照片。 Fig. 1 is a photograph showing the mycelial growth of the control group xylem dicing of the composition of the present invention without the impregnation of the aqueous solution of chlorine dioxide.

第2圖係本發明浸置不同濃度二氧化氯水溶液組成物之實驗組木質部切塊其菌絲生長照片。 Fig. 2 is a photograph showing the mycelial growth of the xylem dicing of the experimental group in which the composition of the present invention was immersed in different concentrations of chlorine dioxide aqueous solution.

第3圖係本發明之控制組、及實驗組其褐根病菌之生長曲線圖。 Fig. 3 is a graph showing the growth curve of the root group of the control group and the experimental group of the present invention.

第4圖係本發明試驗例2浸置不同濃度二氧化氯水溶液組成物之實驗組菌絲生長照片。 Fig. 4 is a photograph showing the hyphal growth of the experimental group in which the composition of the present invention was immersed in different concentrations of chlorine dioxide aqueous solution.

第5圖係本發明3組實驗組與控制組之枇杷樹種苗植入褐根病菌菌母經保鮮膜包覆處理2週後之生長照片,及健康組植入無菌麥粒培養基2經保鮮膜包覆處理2週後之生長照片。 Fig. 5 is a photograph showing the growth of the seedlings of the three groups of the experimental group and the control group of the present invention after being implanted with the wrap of the Rhizoctonia solani for 2 weeks, and the healthy group was implanted with the sterile wheat grain medium 2 through the plastic wrap. Growth photographs after 2 weeks of coating treatment.

第6圖係本發明3組實驗組、控制組與健康組之枇杷樹種苗澆灌二氧化露水溶液16天後之生長照片。 Fig. 6 is a photograph showing the growth of the eucalyptus seedlings of the three experimental groups, the control group and the healthy group of the present invention after 16 days of watering the aqueous solution of the dihydrate.

第7圖係本發明3組實驗組、控制組與健康組之枇杷樹種苗澆灌二氧化露水溶液60天後之生長照片。 Fig. 7 is a photograph showing the growth of the eucalyptus seedlings of the experimental group, the control group and the healthy group of the present invention after 60 days of watering the aqueous solution of the oxidizing dew.

第8圖係本發明3組實驗組、控制組與健康組之枇杷樹種苗澆灌改澆灌一般自來水液60天後之生長照片。 Fig. 8 is a photograph showing the growth of the eucalyptus seedlings of the three experimental groups, the control group and the healthy group of the present invention after 60 days of watering and changing the general tap water.

第9圖係本發明3組實驗組、控制組與健康組之枇杷樹種苗莖幹部切片照片。 Fig. 9 is a photograph showing the stem section of the eucalyptus seedlings of the three experimental groups, the control group and the healthy group of the present invention.

有關本發明為達到目的所運用之技術手段詳細,說明如下:通常根據本發明,該最佳組成物係由二氧化氯(ClO2)氣體溶於水中使形成二氧化氯水溶液組成物,較佳的是,該二氧化氯氣體(ClO2)係電解氯化鈉(NaCl)水溶液所產生,以取得無其他雜質之二氧化氯氣體(ClO2);將該二氧化氯(ClO2)氣體溶於水中形成二氧化氯水溶液之組成物後,可用以治療樹木之褐根病,以作為有效治療防除樹木褐根病之組成物。 DETAILED DESCRIPTION OF THE INVENTION The technical means for achieving the object of the present invention are described in detail below. Generally, according to the present invention, the optimum composition is prepared by dissolving chlorine dioxide (ClO 2 ) gas in water to form a chlorine dioxide aqueous solution composition. The chlorine dioxide gas (ClO 2 ) is produced by electrolytic sodium chloride (NaCl) aqueous solution to obtain chlorine dioxide gas (ClO 2 ) without other impurities; the chlorine dioxide (ClO 2 ) gas is dissolved. After forming a composition of an aqueous solution of chlorine dioxide in water, it can be used to treat brown root disease of trees as a component for effectively treating and controlling brown root disease of trees.

該二氧化氯水溶液組成物具有殺菌、消毒之功效,尤其二氧化氯對微生物細胞壁有較強的吸附穿透能力,可有效地氧化細胞內的酶,因此針對樹木根部、植栽之褐根病菌,其可有效抑制防除褐根病菌之生長,並可殺滅寄生於樹木根部與植栽之褐根病菌,使樹木、植栽不致持續褐化、萎凋死亡;尤其,該二氧化氯水溶液組成物並未對樹木、植栽產生抑制、干擾或殺滅效果或其他化學作用,使樹木、植栽得以存活者。 The chlorine dioxide aqueous solution composition has the functions of sterilization and disinfection, in particular, the chlorine dioxide has strong adsorption and penetration ability to the microbial cell wall, and can effectively oxidize the enzyme in the cell, so the root root and the planted brown root pathogen are planted. It can effectively inhibit the growth of the root-inhibiting bacteria, and can kill the brown root pathogens that are parasitic on the roots of the trees and the plants, so that the trees and plants are not browned and withered and died; in particular, the chlorine dioxide aqueous solution composition It does not cause any inhibition or interference or killing effect on trees or planting, or other chemical effects, so that trees and plants can survive.

對於上述該二氧化氯水溶液組成物所使用之樹種、植栽並無特別限制,舉凡龍眼、荔枝、蕃荔枝、梅樹、枇杷、軟枝番荔枝、可可、百香果、破布子…等果樹,或如茉莉花、黃槐、黃花夾竹桃、金露花、西洋杜鵑、聖誕紅、櫻花、黃金風鈴木、環境綠化樹木、公園行道樹、橡膠樹、茶樹、油椰子、椰子及速生相思樹…等觀賞用樹木,或如草莓、西瓜、苦瓜、鳳梨、芋頭…等植栽,皆可利用二氧化氯水溶液組成物作為有效治療防除褐根病菌之組成物,以殺滅消除樹木根部之褐根病菌,並讓罹病樹 木、植栽可繼續存活而不致褐化、萎凋死亡者。 The tree species and planting used in the above-mentioned composition of the aqueous chlorine dioxide solution are not particularly limited, and the fruit trees such as longan, lychee, lychee, plum, medlar, soft lychee, cocoa, passion fruit, rags, etc. Or ornamental trees such as jasmine, scutellaria, yellow oleander, golden dew, western rhododendron, Christmas red, cherry blossom, golden wind chime, environmental greening trees, park street trees, rubber trees, tea trees, oil coconuts, coconuts and fast-growing acacia trees, or Such as strawberry, watermelon, bitter gourd, pineapple, taro, etc., can use the chlorine dioxide aqueous solution composition as an effective treatment for the prevention of brown root disease bacteria to kill the root roots of the roots, and let the diseased tree Wood and planting can continue to survive without browning and withering death.

關於本發明組成物防除樹木、植栽褐根病菌之處理方法,並無特別限制,例如樹體注入處理、樹冠下土壤處理、莖葉處理、塗布處理…等方法,皆可使用;而上述樹體注入處理係將二氧化氯水溶液組成物注入樹幹或其根系,使二氧化氯水溶液組成物可經循環以抑制殺滅褐根病菌;該樹冠下土壤處理係將二氧化氯水溶液組成物以人工或機器散布、灌注於樹冠、植栽下之土壤,使二氧化氯水溶液組成物可滲透至樹木、植栽根部以抑制殺滅褐根病菌;該莖葉處理係將二氧化氯水溶液組成物以人工或機器散布、灌注於樹木、植栽之莖、葉上,使二氧化氯水溶液組成物可經循環以抑制殺滅褐根病菌;該塗布處理係將二氧化氯水溶液組成物調製成適當濃度,以塗布於樹木、植栽患病處,以抑制殺滅褐根病菌。不論是使用何種方法,其皆將二氧化氯水溶液組成物作為有效成份滲透至樹木、植栽內,藉以防除殺滅褐根病菌,因此可利用局部處理的方法達成治療防除的效果,尤其,利用二氧化氯水溶液組成物防除了可殺滅防除寄生於樹木根部、植栽褐根病菌外,因不使用其他化學藥劑來處理,使樹木、植栽不因化學藥劑之影響而死亡,進而得繼續存活,進而可避免具紀念性、商業性的樹木、特有種或稀有品種的樹木、植栽因感染褐根病菌而褐化、萎凋死亡者。 The method for controlling the plant according to the present invention for controlling trees and planting brown root pathogens is not particularly limited, and examples thereof include a method of injecting a tree, a soil treatment under a canopy, a treatment of stems and leaves, a coating treatment, and the like; The body injection treatment injects the chlorine dioxide aqueous solution composition into the trunk or its root system, so that the chlorine dioxide aqueous solution composition can be circulated to inhibit the killing of the brown root pathogen; the soil treatment under the canopy is to artificially prepare the chlorine dioxide aqueous solution composition. Or the machine is scattered, perfused in the canopy, and the soil under the planting, so that the chlorine dioxide aqueous solution composition can penetrate into the tree and the root of the plant to inhibit the killing of the brown root pathogen; the stem and leaf treatment is to form the chlorine dioxide aqueous solution composition. Artificially or mechanically dispersed or infused into trees, stems and leaves of plants, so that the composition of the aqueous solution of chlorine dioxide can be circulated to inhibit the killing of Rhizoctonia solani; the coating treatment is to prepare the aqueous solution of chlorine dioxide to an appropriate concentration. In order to coat the trees and plant the diseased area to inhibit the killing of the brown root pathogen. Regardless of the method used, the chlorine dioxide aqueous solution composition is infiltrated into trees and plants as an active ingredient, thereby preventing the killing of the brown root pathogen, so that the local treatment method can be used to achieve the therapeutic control effect, in particular, The use of the chlorine dioxide aqueous solution composition can prevent the parasitic roots of the trees and the planting of the brown root pathogens, and does not use other chemical agents to treat, so that the trees and plants are not killed by the influence of chemical agents, and thus Continue to survive, and then avoid commemorative, commercial trees, endemic or rare species of trees, planting browning due to infection with brown root pathogens, and withering death.

本發明之二氧化氯水溶液組成物,除了可作為有效治療罹患褐根病菌樹木、植栽之成份,以防除樹木、植栽褐根病菌之生長並予以殺滅外,該二氧化氯水溶液組成物亦可預先處理使用使滲透於未感染褐根病菌之樹木、植栽內,預防健康之樹木、植栽因接觸罹換褐根病樹木、植栽 根系,或接觸病苗、病土而感染褐根病菌;並可於樹木、植栽受感染或未確認感染褐根病菌前之初期處理,以達可即時消滅防除並抑制褐根病菌之生長,使樹木、植栽不致感染、惡化而褐化萎凋,進而可有效預防樹木、植栽感染褐根病菌。關於本發明組成物預防樹木、植栽褐根病菌之處理方法,並無特別限制,例如樹體注入處理、樹冠下土壤處理、莖葉處理、塗布處理…等方法,皆可使用。 The chlorine dioxide aqueous solution composition of the present invention can be used as an effective treatment for the diseases of the trees and planting of the brown root pathogen to prevent the growth and killing of the tree and the planting rhizogenes, and the chlorine dioxide aqueous solution composition It can also be pre-treated to use trees that are infiltrated into uninfected brown root pathogens, planted in plants, to prevent health, and to plant plants for contact with alfalfa for brown root disease trees. Roots, or contact with diseased seedlings and diseased soils to infect brown root pathogens; and can be treated in the early stages before trees, planting infections, or unidentified brown root pathogens, so as to instantly eliminate and inhibit the growth of brown root pathogens. Trees and plants are not infected, deteriorated, and browned and withered, which can effectively prevent trees and plants from infecting brown root pathogens. The method for treating trees and planting brown root pathogens of the composition of the present invention is not particularly limited, and examples thereof include a tree infusion treatment, a soil treatment under the canopy, a stem and leaf treatment, a coating treatment, and the like.

為證明本發明之二氧化氯水溶液組成物確具有殺滅防除褐根病菌,發明人即以下試驗例以茲證明二氧化氯水溶液組成物防除褐根病菌之效果: In order to prove that the composition of the aqueous chlorine dioxide solution of the present invention has the function of killing and preventing brown root disease, the inventors, the following test examples, have proved that the composition of the aqueous chlorine dioxide solution has the effect of controlling the brown root pathogen:

試驗例1 Test example 1

取得帶有褐根病菌之木質部切塊,並將該木質部切塊經分離、純化等步驟後,使該木質部切塊上僅有褐根菌菌絲而無其他雜菌。木質部切塊進一步培養褐根菌,並取得純化褐根病菌,培養並貯存。由取得純化之褐根病菌於PDA固態培養基上培養長出菌絲團,由菌絲團取出菌絲塊。 The xylem dicing with the brown root pathogen is obtained, and the xylem diced piece is separated and purified, and then the xylem portion has only the brown fungus hyphae and no other bacteria. The xylem stalk was further cultivated, and the purified brown root pathogen was obtained, cultured and stored. The mycelium was grown by culturing the purified Rhizoctonia solani on PDA solid medium, and the mycelial mass was taken out from the mycelium.

將褐根病菌菌絲塊分別浸泡於濃度為20ppm、200ppm或2000ppm二氧化氯水溶液組成物中15分鐘後,取出菌絲塊貼附於9cm PDA固態培養基上培養為實驗組,同時取褐根病菌菌絲塊貼附於9cm PDA固態培養基上作為控制組,該控制組並未浸置有二氧化氯水溶液組成物;觀察10天後該帶有褐根病菌之菌絲塊生長情形。 After soaking the hyphae of the Rhizoctonia solani in the concentration of 20ppm, 200ppm or 2000ppm chlorine dioxide solution for 15 minutes, the hyphae were taken out and attached to the 9cm PDA solid medium to be cultured as the experimental group, and the brown root pathogen was taken. The mycelial mass was attached to a 9 cm PDA solid medium as a control group, and the control group was not immersed with a chlorine dioxide aqueous solution composition; the growth of the mycelium with brown root pathogen was observed 10 days later.

如第1圖所示,該未浸置二氧化氯水溶液組成物之控制組,其褐根病菌菌絲塊之菌絲已生長滿佈於培養盤(如圖所示白色之菌絲)。 As shown in Fig. 1, in the control group of the unsoaked chlorine dioxide aqueous solution composition, the hyphae of the Rhizoctonia solani hyphae have been grown on the culture tray (white hyphae as shown).

如第2圖所示,該浸置於濃度為20ppm、200ppm或2000ppm二氧化氯水溶液組成物中之褐根病菌菌絲塊,則菌絲塊上之褐根病菌明顯未生長。 As shown in Fig. 2, the brown root pathogen on the hyphae was not grown in the hyphae of the Rhizoctonia solani in the composition of the aqueous solution of chlorine dioxide of 20 ppm, 200 ppm or 2000 ppm.

由上述試驗例1及第1、2圖之照片,及第3圖之生長曲線圖可知,該未浸置二氧化氯水溶液組成物之褐根病菌菌絲塊,其於第8天時,該褐根病菌之菌絲已生長滿佈於直徑為9cm之培養盤,而浸置於二氧化氯水溶液組成物中之褐根病菌菌絲塊,其觀察至第10天時,褐根病菌之菌絲塊明顯沒有菌絲生長擴散;因此,該二氧化氯水溶液組成物確可有效抑制褐根病菌之生長,且可殺滅消除菌絲塊上之褐根病菌。 From the above-mentioned test example 1 and the photographs of the first and second graphs, and the growth graph of the third graph, it can be seen that the brown root pathogen hyphae of the unsoaked chlorine dioxide aqueous solution composition is on the eighth day, The mycelium of Rhizoctonia solani has been grown in a culture plate with a diameter of 9 cm, and the hyphae of the Rhizoctonia solani in the composition of the aqueous solution of chlorine dioxide was observed. On the 10th day, the bacteria of the root pathogen were observed. The silk block obviously has no mycelial growth and diffusion; therefore, the chlorine dioxide aqueous solution composition can effectively inhibit the growth of the brown root pathogen, and can kill the brown root pathogen on the hyphae.

試驗例2 Test example 2

於白色稍微褐化的褐根菌上以手術刀切下約0.5~1.0cm2的菌絲塊數塊放置於液態培養基(1.5g Potato Dextrose Broth/50mL H2O)靜置培養2~3天,待長出菌絲後,使用超音波探針對菌液進行超音波震盪1分鐘,取得已震碎菌絲的懸浮液體,與不同濃度(0、20、200、1000ppm)的二氧化氯水溶液組成物以1:1(懸浮液體:二氧化氯水溶液組成物)體積混合並靜置20分鐘;然後,分別取50μL混合液體置於新鮮的PDA平板培養基(3g Potato Dextrose Broth,1.5g Agar/100mL H2O)中央,封膜後存放培養3天,並觀察其菌絲生長情形。 A few pieces of hyphae cut about 0.5-1.0 cm 2 were placed on a white-brown brown fungus with a scalpel and placed in a liquid medium (1.5 g Potato Dextrose Broth/50 mL H 2 O) for 2 to 3 days. After the hyphae are grown, the ultrasonic solution is used to ultrasonically oscillate the bacterial liquid for 1 minute to obtain a suspended liquid of the shredded hyphae, which is composed of a different concentration (0, 20, 200, 1000 ppm) of chlorine dioxide aqueous solution. The mixture was mixed in a volume of 1:1 (suspended liquid: aqueous chlorine dioxide solution) and allowed to stand for 20 minutes; then, 50 μL of the mixed liquid was placed in fresh PDA plate medium (3 g Potato Dextrose Broth, 1.5 g Agar/100 mL H). 2 O) Central, after storage, store for 3 days, and observe the mycelial growth.

如第4圖所示,該無二氧化氯水溶液組成物(0ppm)處理的褐根病菌其生長直徑為2.0cm、該10ppm二氧化氯水溶液組成物處理的褐根病菌其生長直徑為1.5cm、該100ppm二氧化氯水溶液組成物處理的褐根病菌其生長直徑為1.5cm、該500ppm二氧化氯水溶液組成物處理的褐 根病菌其生長直徑為0cm;同時,該10ppm二氧化氯水溶液組成物處理的褐根病菌的生長厚度也明顯比0ppm二氧化氯水溶液組成物處理的褐根病菌的生長厚度薄、該100ppm二氧化氯水溶液組成物處理的褐根病菌的生長厚度也明顯比10ppm二氧化氯水溶液組成物處理的褐根病菌的生長厚度為薄,而該500ppm二氧化氯水溶液組成物則可完全抑制褐根病菌之生長者。 As shown in Fig. 4, the Rhizoctonia solani treated with the chlorine dioxide-free aqueous solution composition (0 ppm) has a growth diameter of 2.0 cm, and the Rhizoctonia solani treated with the 10 ppm aqueous chlorine dioxide solution has a growth diameter of 1.5 cm. The brown root pathogen treated by the 100 ppm aqueous solution of chlorine dioxide has a growth diameter of 1.5 cm, and the treated body of the 500 ppm aqueous chlorine dioxide solution is brown. The root pathogen has a growth diameter of 0 cm; at the same time, the growth thickness of the Rhizoctonia solani treated with the 10 ppm chlorine dioxide aqueous solution composition is also significantly thinner than the growth thickness of the 0 ppm chlorine dioxide aqueous solution treated brown root pathogen, the 100 ppm dioxide The growth thickness of the Rhizoctonia solani treated with the chlorine solution composition is also significantly thinner than the growth thickness of the Rhizoctonia solani treated with the 10 ppm chlorine dioxide aqueous solution composition, and the 500 ppm chlorine dioxide aqueous solution composition can completely inhibit the brown root pathogen Growing people.

實例 Instance

培養菌母與感染處理:取0.5cm2褐根病菌菌絲塊數塊放置於麥粒培養基(取25g小麥、25g燕麥、25mL RO水),於24℃下恆溫培養約1個月使形成菌母,然後取4株1~2年生健康之枇杷樹種苗,在其中4株枇杷樹種苗位於泥土上方之莖幹上刮去基部表皮使形成傷口,在該傷口植入上述褐根病菌之菌母,並以保鮮膜包覆傷口置放2週,使該4株枇杷樹種苗感染褐根病菌,藉以令其中1株作為控制組,其中3株分別作為A、B、C實驗組;取另一株1~2年生健康之枇杷樹種苗,同樣於泥土上方之莖幹上刮去基部表皮形成傷口,在該傷口植入無菌之麥粒培養基,藉以作為無感染褐根病菌之健康組。 Culture of bacterial mother and infection treatment: Take 0.5cm 2 of Rhizoctonia solani mycelium and place it in wheat grain medium (take 25g wheat, 25g oat, 25mL RO water), and incubate at 24 °C for about 1 month to form bacteria. Mother, then take 4 strains of eucalyptus seedlings of 1~2 years old, and scrape off the basal epidermis on the stems of the four eucalyptus seedlings above the soil to form a wound, and implant the above-mentioned bacterial root of the brown root pathogen And the wounds were covered with plastic wrap for 2 weeks, so that the four eucalyptus seedlings were infected with the brown root pathogen, so that one of them was used as the control group, and three of them were used as the experimental group of A, B and C respectively; The eucalyptus seedlings of 1 to 2 years old are also scraped off the basal epidermis to form a wound on the stem above the soil, and the sterile wheat grain medium is implanted in the wound as a healthy group without infected brown root pathogen.

二氧化氯水溶液組成物澆灌處理:調配濃度分別為20ppm、200ppm、1000ppm之二氧化氯水溶液組成物,令A實驗組每4日澆灌濃度20ppm之200mL二氧化氯水溶液組成物於根部土壤中,令B實驗組每4日澆灌濃度200ppm之200mL二氧化氯水溶液組成物於根部土壤中,令C實驗組每4日澆灌濃度1000ppm之200mL二氧化氯水溶液組成物於根部土壤中,而該控制組與健康組則每4日澆灌200mL之一般自來水。經澆灌二氧化氯水溶液組成物90天後,A、B、C實驗組改以一般自來水 澆灌。觀察A、B、C實驗組與控制組、健康組枇杷樹種苗之生長情形,並記錄之。 Watering treatment of chlorine dioxide aqueous solution composition: compounding a concentration of 20ppm, 200ppm and 1000ppm of chlorine dioxide aqueous solution, respectively, so that the experimental group A watered 20ppm 200mL chlorine dioxide aqueous solution composition in the root soil every 4 days, so that In the experimental group B, 200 ml of a 200 mL aqueous solution of chlorine dioxide was poured into the root soil every 4 days, and the C experimental group was poured with a concentration of 1000 ppm of 200 mL of a chlorine dioxide aqueous solution per 10 days in the root soil, and the control group and the control group were In the health group, 200 mL of normal tap water was poured every 4 days. After 90 days of watering the chlorine dioxide aqueous solution composition, the experimental groups of A, B and C were changed to general tap water. water. Observe the growth of eucalyptus seedlings in the experimental group, control group and healthy group of A, B and C, and record them.

實驗結果: Experimental results:

如第5圖所示,其係A、B、C實驗組與控制組之枇杷樹種苗植入褐根病菌菌母經保鮮膜包覆處理2週後之生長照片。而該健康組則為植入無菌之麥粒培養基經保鮮膜包覆處理2週後之生長照片;由以上照片可知,該A、B、C實驗組與控制組之枇杷樹種苗尚未明顯受褐根病菌之影響。 As shown in Fig. 5, the growth photographs of the eucalyptus seedlings of the experimental group A and B, and the control group were implanted with the wrap of the Rhizoctonia solani for 2 weeks. The healthy group is a photograph of the growth of the sterile wheat grain medium after being coated with plastic wrap for 2 weeks; from the above photos, the seedlings of the A, B, C experimental group and the control group have not been significantly browned. The effect of root bacteria.

如第6圖所示,其係為開始A、B、C實驗組澆灌二氧化氯水溶液組成物16天(澆灌4次)及控制組與健康組澆灌一般自來水16天(澆灌4次)後之生長照片;該控制組與A實驗組之枇杷樹種苗雖未嚴重感染褐根病菌,外觀尚為健康,但其新生葉片生長已開始趨緩,其所生長之葉片尖端有下捲之不大健康的形狀,而B、C實驗組之枇杷樹種苗看起來較無受到褐根病菌之影響,且其新生葉片之生長速度較控制組與A實驗組為快,而健康組則為外觀健康、正常生長。 As shown in Figure 6, it is the beginning of the A, B, C experimental group watering chlorine dioxide aqueous solution composition for 16 days (watering 4 times) and the control group and the health group watering the general tap water for 16 days (watering 4 times) The photos of the growth; the eucalyptus seedlings of the control group and the A experimental group are not seriously infected with the brown root pathogen, and the appearance is still healthy, but the growth of the new leaves has begun to slow down, and the tips of the leaves that grow are not so healthy. The shape of the eucalyptus seedlings in the B and C experimental groups appeared to be less affected by the brown root pathogen, and the growth rate of the new leaves was faster than that of the control group and the A experimental group, while the healthy group was healthy and normal. Growing.

如第7圖所示,其係為A、B、C實驗組澆灌二氧化氯水溶液組成物60天(澆灌15次)及控制組與健康組澆灌一般自來水60天(澆灌15次)後之生長照片;該健康組為外觀健康、健康生長,該A實驗組與控制組之枇杷樹種苗出現生長緩慢、枯黃,顯然已受褐根病菌之影響,而B、C實驗組之枇杷樹種苗之外觀健康,且持續正常生長,其與健康之健康組相差無幾;故,該濃度為200ppm與1000ppm之二氧化氯水溶液組成物不僅未對枇杷樹種苗產生傷害,且顯然可抑制褐根病菌之侵害者。 As shown in Figure 7, it is the A, B, C experimental group watering chlorine dioxide aqueous solution composition for 60 days (15 times watering) and the growth of the control group and the healthy group after pouring ordinary tap water for 60 days (15 times watering) Photo; the healthy group is healthy and healthy, and the eucalyptus seedlings of the experimental group and the control group are slow-growing and yellowish, obviously affected by the brown root pathogen, and the appearance of the eucalyptus seedlings of the B and C experimental groups Healthy and continuous normal growth, which is similar to the healthy health group; therefore, the 200ppm and 1000ppm chlorine dioxide aqueous solution composition not only does not cause damage to the eucalyptus seedlings, and obviously inhibits the aggravation of the brown root pathogen .

如第8圖所示,其係A、B、C實驗組改以一般自來水澆灌30天後之生長照片。控制組與健康組則持續投予自來水;該健康組之枇杷樹種苗則為健康生長,其上緣葉片雖呈半焦黃,其應為日照過多所致,下半部未受強烈日曬之葉片皆為健康綠色葉片可比對,而該A實驗組之枇杷樹種苗迅速枯黃、落葉,新生葉片十分弱小與且容易掉落,亦有新生葉片無法順利發出之情事發生,其生理機能顯著下降;而B、C實驗組之枇杷樹種苗生長良好,其葉片與健康組同樣茂盛且其葉片大小亦相近,且無生長緩慢或變形之不健康反應;而該控制組之枇杷樹種苗不僅枯黃、落葉,葉片生長緩慢又葉片形狀捲曲,且同樣無法順利新生葉片,其受褐根病菌之影響劇烈。 As shown in Fig. 8, the experimental group of the A, B, and C groups was changed to a photograph of growth after 30 days of general tap water pouring. The control group and the health group continued to administer tap water; the eucalyptus seedlings in the healthy group were healthy growth, and the upper leaves were semi-coke yellow, which should be caused by excessive sunlight, and the lower half was not subjected to strong sun exposure. The healthy green leaves are comparable, and the eucalyptus seedlings of the A experimental group are quickly yellow and deciduous, the new leaves are very weak and easy to fall, and the new leaves are not able to be smoothly sent out, and their physiological functions are significantly reduced; The eucalyptus seedlings of the B and C experimental groups grew well, and the leaves were as vigorous as the healthy group and their leaf sizes were similar, and there was no unhealthy reaction with slow growth or deformation; while the control group had not only yellow, deciduous, and leaves The growth is slow and the shape of the leaves is curled, and it is also impossible to smoothly regenerate the leaves, which is strongly affected by the brown root pathogen.

如第9圖所示,其係A、B、C實驗組、控制組與健康組之枇杷樹種苗臨根部之莖幹部切片照片,該感染褐根病菌之樹木會具有顯著之褐色網狀紋路與木質白腐兩大特徵;該A實驗組可看見木質部有清楚的褐色紋路,部分已開始產生木質白腐,顯然濃度為20ppm之二氧化氯水溶液組成物並未能有效抑制褐根病菌之侵害;該B實驗組之褐色網紋不明顯,受到木質白腐的區塊也較A實驗組為小,因此濃度為200ppm之二氧化氯水溶液組成物可抑制褐根病菌之侵害,使其病徵顯著減少;該C實驗組未見有褐色網紋與木質白腐,因此濃度為1000ppm之二氧化氯水溶液組成物顯然可有效抑制褐根病菌之侵害,並可防除褐根病菌者;而控制組之切片有顯眼清楚的褐色粗紋路,並有近50%面積有木質白腐現象,年輪痕跡也因褐根菌侵蝕而模糊不可辨認;而該健康組則完全無產生褐色網狀紋路與木質白腐,且其年輪清晰可見。 As shown in Figure 9, the photographs of the stems of the roots of the eucalyptus seedlings in the experimental group, the control group and the healthy group of the A, B, and C groups, the trees infected with the brown root pathogen have a significant brown mesh pattern and Two characteristics of wood white rot; the A experimental group can see that the xylem has clear brown lines, and some of it has begun to produce wood white rot. It is obvious that the composition of 20ppm chlorine dioxide aqueous solution can not effectively inhibit the damage of brown root pathogen; The brown netting of the B experimental group is not obvious, and the block of wood white rot is also smaller than that of the A experimental group. Therefore, the composition of the 200 ppm aqueous solution of chlorine dioxide can inhibit the damage of the brown root pathogen and significantly reduce the symptoms. There was no brown netting and woody white rot in the C experimental group. Therefore, the composition of the aqueous solution of chlorine dioxide at a concentration of 1000 ppm was obviously effective in inhibiting the damage of the brown root pathogen and preventing the brown root pathogen; and the slice of the control group There are conspicuous brown rough lines, and nearly 50% of the area has wood white rot. The annual ring marks are also obscured by brown root bacteria erosion; while the healthy group has no brown mesh at all. Road and white rot of wood, and which ring is clearly visible.

由上述褐根病菌體外抑制試驗之兩試驗例與帶褐根病菌枇杷樹種苗之實例可知,該作為治療防除褐根病菌有效組成物之二氧化氯水溶液組成物,於褐根病菌體外抑制試驗之實驗二例,於10至20ppm之二氧化氯水溶液組成物即可見明顯的抑制褐根病菌絲之成長,隨著二氧化氯水溶液組成物濃度之升高其抑制褐根病菌絲之成長效果也越明顯。於1000ppm之二氧化氯水溶液組成物顯然可完全抑制褐根病菌絲之成長。而褐根病菌感染枇杷樹種苗的實驗模式為一植栽感染褐根病菌之急性模式,這種模式並不相同於一般田間或野外褐根病菌感染植物的慢性感染方式,其植物於感染褐根病菌的過程是緩慢的,其症狀的顯示也是緩慢的;所以,於本感染褐根病菌之急性模式的植栽於短時間即出現出明顯且嚴重的症狀,類似於一般田間或野外褐根病菌感染植物之中、末期徵狀。故,由上述實驗可知高濃度的二氧化氯水溶液組成物才能有效的防治褐根病菌之侵害,而濃度20ppm對枇杷樹種苗雖無完全抑制褐根病菌之效果,但也顯示出其可稍微延緩褐根病症狀進展之效用;隨著二氧化氯水溶液組成物濃度之升高其抑制褐根病症狀效果也越明顯。於1000ppm之二氧化氯水溶液組成物顯然可有效抑制褐根病菌之侵害。因此二氧化氯水溶液組成物即可有效抑制褐根病菌對樹木、植栽之影響,且可防止褐根病菌於樹木、植栽中的惡化、擴散,並對該褐根病菌抑制殺滅,實可有效防除樹木褐根病菌者;同時,其可依據樹木、植栽罹患褐根病之程度與樹木、植栽之品種、生長大小予以調整二氧化氯水溶液組成物之濃度,藉以提高二氧化氯水溶液組成物抑制殺滅褐根病菌之效果。 From the two test examples of the in vitro inhibition test of the brown root pathogen and the example of the seedling of the eucalyptus seedling, the composition of the chlorine dioxide aqueous solution as an effective component for treating the brown root disease preventing bacteria is inhibited in vitro by the brown root disease bacteria. In the second experiment, the composition of the aqueous solution of chlorine dioxide in the range of 10 to 20 ppm can obviously inhibit the growth of the hyphae of brown root disease. With the increase of the concentration of the aqueous solution of chlorine dioxide, the effect of inhibiting the growth of the rhizomes of brown root disease is also more obvious. The composition of the aqueous solution of chlorine dioxide at 1000 ppm apparently completely inhibited the growth of the rhizomes of the rhizosphere. The experimental model of the infection of eucalyptus seedlings with brown root pathogen is an acute mode of planting brown root pathogens. This model is not the same as the chronic infection of plants infected with brown root pathogens in the field or in the field. The process of pathogens is slow, and the symptoms are also slow to show; therefore, the planting of the acute mode of the Rhizoctonia solani has obvious and serious symptoms in a short period of time, similar to the general field or wild brown root pathogens. Infected plants, terminal symptoms. Therefore, it can be seen from the above experiments that the high concentration of the chlorine dioxide aqueous solution composition can effectively prevent the damage of the brown root pathogen, while the concentration of 20 ppm does not completely inhibit the effect of the brown root pathogen on the eucalyptus seedlings, but it also shows that it can be slightly delayed. The effect of the symptoms of brown root disease is progressing; the effect of inhibiting the symptoms of brown root disease is more obvious as the concentration of the aqueous solution of chlorine dioxide increases. The composition of the aqueous solution of chlorine dioxide at 1000 ppm is obviously effective for inhibiting the damage of the brown root pathogen. Therefore, the composition of the aqueous solution of chlorine dioxide can effectively inhibit the effects of the brown root pathogen on trees and planting, and can prevent the deterioration and spread of the brown root pathogen in trees and plants, and inhibit the killing of the brown root pathogen. It can effectively control the root brown fungus of trees; at the same time, it can adjust the concentration of chlorine dioxide aqueous solution composition according to the degree of tree roots, planting and suffering of brown root disease and the size of trees, planting varieties and growth, so as to increase chlorine dioxide. The aqueous solution composition inhibits the effect of killing the brown root pathogen.

另,本發明之二氧化氯水溶液組成物並可視情況添加營養劑 或肥料,使在殺滅抑制褐根病菌的期間,樹木、植栽可同步吸收營養劑或肥料,以提高樹木、植栽之生命力及抵抗力,使罹患褐根病菌之樹木、植栽可藉由二氧化氯水溶液組成物殺滅防除褐根病菌,並可利用營養劑補充生長營養,使樹木確可有效存活而不致萎凋死亡。 In addition, the chlorine dioxide aqueous solution composition of the present invention may optionally be added with a nutrient Or fertilizer, so that during the period of killing and inhibiting Rhizoctonia solani, trees and plants can simultaneously absorb nutrients or fertilizers to improve the vitality and resistance of trees and plants, so that trees and plants that can bear brown root pathogens can be borrowed. The composition of the aqueous solution of chlorine dioxide kills and prevents the rooting bacteria, and can supplement the growth nutrients with nutrients, so that the trees can survive effectively without dying.

又,由上述實驗例之實驗組枇杷樹種苗可知,澆灌二氧化氯水溶液組成物並未對枇杷樹種苗之生理機能、葉片生長產生影響,因此,本發明之二氧化氯水溶液組成物確可有效使樹木有效防除褐根病菌,避免感染、惡化而褐化萎凋者。 Moreover, it can be seen from the experimental group eucalyptus seedlings that the composition of the aqueous chlorine dioxide solution does not affect the physiological function and leaf growth of the eucalyptus seedlings, and therefore, the chlorine dioxide aqueous solution composition of the present invention is effective. Make trees effective against brown root pathogens, avoid infection, deterioration and browning.

綜上所陳,本發明所運用之二氧化氯水溶液組成物及防除方法,確可有效防除樹木、植栽之褐根病菌,亦可避免健康之樹木、植栽感染褐根病菌;因此,本發明相較於先前以掘溝阻斷法、燻蒸劑處理病土法、淹水處理法、藥劑混土覆蓋法或稀釋灌注法…等方式為佳,且本發明之二氧化氯水溶液組成物亦未影響樹木、植栽之生理機能,因此本發明確具有顯著之進步性,且為未曾有過,誠已符合發明專利要件,爰依法提出專利申請,並祈賜專利為禱,至感德便。 In summary, the chlorine dioxide aqueous solution composition and the control method used in the present invention can effectively prevent trees and planted brown root pathogens, and can also avoid healthy trees and planting infected brown root pathogens; therefore, this The invention is better than the previous method of trenching blocking method, fumigant treatment soil method, flooding treatment method, chemical soil covering method or dilution infusion method, etc., and the chlorine dioxide aqueous solution composition of the invention is also The invention does not affect the physiological functions of trees and planting. Therefore, the present invention has significant progress, and it has never been before. Cheng has already complied with the patent requirements of the invention, and filed a patent application according to law, and prayed for a patent for prayer.

惟以上所述者,僅為本發明之一可行實施例,因此並不能以之限定本發明實施之範圍,舉凡依本發明說明書及申請專利範圍所為之等效變化與修飾,皆應仍屬本發明所涵蓋之範圍內。 However, the above description is only one of the possible embodiments of the present invention, and thus the scope of the present invention is not limited thereto, and the equivalent changes and modifications according to the description of the present invention and the scope of the patent application should still belong to the present invention. Within the scope of the invention.

Claims (4)

一種防除樹木、植栽褐根病菌之組成物,其係將二氧化氯氣體溶於水中使形成二氧化氯水溶液組成物;利用該二氧化氯水溶液組成物處理感染褐根病菌之樹木、植栽,藉以防除殺滅寄生於樹木根部、植栽之褐根病菌,使感染之樹木、植栽可存活而不致萎凋死亡,且可處理未感染褐根病菌之樹木、植栽,以有效防除褐根病菌寄生於樹木根部、植栽上。 The invention relates to a composition for controlling tree roots and planting brown root path bacteria, which dissolves chlorine dioxide gas in water to form a chlorine dioxide aqueous solution composition; and uses the chlorine dioxide aqueous solution composition to treat trees infected with brown root path bacteria and plant In order to prevent the killing of the roots of the trees and the planting of the brown root pathogen, so that the infected trees and plants can survive without dying, and can treat the trees and plants that are not infected with the brown root pathogen, so as to effectively prevent brown roots. The pathogen is parasitic on the roots of the trees and planted. 如申請專利範圍第1項所述之一種防除樹木、植栽褐根病之組成物,其中該二氧化氯氣體係經電解氯化鈉水溶液所產生為佳。 A composition for controlling tree rooting and planting brown root disease according to claim 1, wherein the chlorine dioxide gas system is preferably produced by electrolytic sodium chloride aqueous solution. 一種防除樹木、植栽褐根病菌之方法,其係以利用上述申請專利範圍第1項所述之二氧化氯水溶液組成物作為有效成份處理罹患褐根病菌之樹木、植栽,以防除殺滅寄生於樹木根部之褐根病菌,達到有效治療樹木、植栽之效果。 The invention relates to a method for controlling trees and planting brown root pathogens, which comprises treating the trees and plants planted with brown root pathogens by using the chlorine dioxide aqueous solution composition described in item 1 of the above patent application as an effective ingredient to prevent and kill The brown root pathogen that is parasitic on the roots of trees can effectively treat trees and plant. 一種保護樹木、植栽之方法,其係以利用上述申請專利範圍第1項所述之二氧化氯水溶液組成物作為有效成份處理樹木、植栽,以防除褐根病菌寄生於樹木根部,達到有效預防樹木感染褐根病菌之效果。 The invention relates to a method for protecting trees and planting plants, which comprises treating the trees and planting with the chlorine dioxide aqueous solution composition described in Item 1 of the above-mentioned patent application as an active ingredient, so as to prevent parasitic root bacteria from parasitic on the roots of the trees, and achieve effective Prevent the effects of tree-infected brown root bacteria.
TW103100473A 2013-01-09 2014-01-07 The invention is described the use of chlorine dioxide solution to prevent or cure brown root rot disease of plants TW201427595A (en)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
TWI732483B (en) * 2020-03-12 2021-07-01 孫岩章 Shallow hole injection of medicament to treat brown root disease of trees
TWI766712B (en) * 2021-06-01 2022-06-01 行政院農業委員會特有生物研究保育中心 Use of marjoram essential oil and brown root rot prevention and treatment employing the same

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
TWI732483B (en) * 2020-03-12 2021-07-01 孫岩章 Shallow hole injection of medicament to treat brown root disease of trees
TWI766712B (en) * 2021-06-01 2022-06-01 行政院農業委員會特有生物研究保育中心 Use of marjoram essential oil and brown root rot prevention and treatment employing the same

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