201143803 六、發明說明： 【發明所屬之技術領域】 本發明為提供一種毛髮生長促進成分’尤指一種 可維持毛髮生長以及增加其濃密程度之成分，可使用 於哺乳類動物之皮膚或頭皮。 【先前技術】 由於現今人口老齡化以及生活壓力曰與倶增，愈來 •愈多人正面臨落髮或禿頭之危機，根據醫學研究指出此 危機也造成了家庭情緒與心理之壓力，故大多數人嘗試 找到有效治療先頭之方法；普遍來說，毛髮脫落是由於 男性射爾蒙與毛囊中之二氫睾酮所引起，二氫睾酮會減 少毛囊與毛髮真皮乳狀突起之金流量，使頭皮養份不足 或產生氧化反應，故一般生髮水配方具有消除或緩和上 述症狀之成分，例如加上獐牙菜萃取液、維生素E及其 鲁衍生物或以酰膽驗衍生物以增加頭皮之血液循環，或加 入胺基酸如絲氨酸或蛋氨酸、維生素如B6等滋養毛囊 配方以預防脫髮，促進頭髮生長。 美國食品藥物管理局（FDA)提出兩種治療男性禿 頭之成为，分別為f inasteride(非那雄胺，美國專利 證號 2006/0099251 A1)以及 minoxidil(米諾地爾， 美國專利證號4139619) ; finasteride(非那雄胺） 係利用抑制活性的5 還原酶使自由睾酮轉化為二 201143803 氫睾酮，引發毛囊細微化（Dall〇b，1994年），其原本 使用於治療良性前列腺增生症（BPH)，但後來發現也可 以有效地治療男性禿頭；minoxidil(米諾地爾）是一種 血管擴張劑’本來為一種可治療高血壓之口服藥物 (Loniten) ’於1980年代時被發現其副作用可使頭髮 生長與抑制落髮；普強公司獲得FDA批准上市一種產 含2% minoxidil(米諾地爾）之產品用來治療禿頭和落 髮（RogaineandRegaine)，有效之原因可能透過擴張 血管與開放鉀通道，導致更多的氧氣、血液和養分進入 毛囊中；minoxidil(米諾地爾）生髮水被建議與腺苷一 起使用（Li ’2001年）’並以A2b腺苷作為受體途徑 (lino，2007年）’故揭露了腺苷也具有治療禿頭之功 能’但可能會有副作用發生，尤其是當minoxidil(米 諾地爾）與腺苷一起使用時，可能發生水腫、心動過快、 呼吸困難、疲勞與噁心’並可能會導致癌症之形成。 雖然已經嘗試如上述之各種方法，但目前市面上之 生髮水未必於頭皮上任何區域皆具有足夠地防止落髮 與促進頭髮生長之能力，舉例來說，使用finasteride (非那雄胺）與minoxidil (米諾地爾）於頭頂或前額 處時，幾乎只有頭頂處可有效生出毛髮；此外，大部份 治療必須經過很長的時間才會有效果出現，例如4至6 個月；治療禿頭也可透過移植毛髮手術解決此問題，但 201143803 手術過程中會感到疼痛且無法保證一定會成功，頭髮甚 至需要等待數月至數年才會再生。 故’上述問題，將是在此領域技術者所欲解決之 困難所在。 【發明内容】 故，本發明之發明人有鑒於上述先前技術所述之 不足’提出-種毛髮生長促進成分，其主要具有下列 • 之目的·· 本發明之主要目的在於：本發明之毛髮生長促進 成分，透過適量之腺嗓吟或腺嗓吟鹽類作為活性物質 以及化妝品載體，可提供不傷害人體皮膚或頭皮之生 髮水、潔髮乳液、潤髮液、毛髮護理凝膠、頭髮定型 液或洗髮水，其不僅具有刺激毛髮生長、防止落髮， 甚至也有促進眼睫毛與眉毛生長之功能。 •【實施方式】 為了達成上述各項目的及功效，於此謹搭配圖 式，舉一較佳實施例，俾便在此領域中具通常知識者 能夠就各項目的據以實施。 首先，本發明係提供一種毛髮生長促進成分其 主要包含適量之腺嘌呤或腺嘌呤鹽類作為活性物質以 及化妝品載體’其活性物質的比例會因配方形態、製 作方法而有所不同’其佔成分重量百分比之較佳範園 201143803 為0. 0001%以上，該毛髮生長促進成分可以生髮水、潔 髮乳液、潤髮液、毛髮護理凝膠、頭髮定型液或洗髮 水等形式存在，並以液體、膏狀或乳膠狀塗抹於皮膚 或頭皮；此外，本發明包含一化妝品载體，可為稀釋 劑、分散劑或腺嘌呤之載體，以促進毛髮生長促進成 分之配方平均分佈於皮膚表面，載體之形態可為液體 或固體潤滑劑、溶劑、保濕劑、增稠劑或粉末；除了 本發明之活性物質與化妝品載體外，毛髮生長促進成 分也包含常使用於化妝品、藥物或皮膚科領域中之添 加劑和佐劑，如親水性或親油性凝膠劑、親水或親油 活性劑、防腐劑、抗氧化劑、溶劑、香料、填料、殺 菌劑、除臭劑、染料或著色劑，這些添加劑和佐劑的 使用範圍由總重量百分比之0 01%至10%組成。 為了證明本發明中腺嗓呤或腺嗓吟鹽類含量對於 毛髮生長能力之影響，本發明之毛髮生長促進成分以不 :比例配置出四種樣本之生髮水溶液，其比例成分由表 一所不，其樣本配置流程步驟方法包括： ⑴將腺切硫酸與、薄荷醇、聚乙二醇普氫化萬麻 油、乙醇以及部分離子交換水混合、㈣後產生一 溶液； ⑺再將其餘的離子交換水，添加至上述溶液中，以形 成生髮水溶液。 201143803 生髮水配置樣本1-4 樣本1 樣本2 樣本3 樣本4 控制組 % (w/w)BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention provides a hair growth promoting component, particularly a component which can maintain hair growth and increase its density, and can be used for the skin or scalp of a mammal. [Prior Art] Due to the aging of the population today and the increasing pressure on life, more and more people are facing the crisis of hair loss or baldness. According to medical research, this crisis has also caused family emotional and psychological stress, so most of them People try to find a way to effectively treat the head; in general, hair loss is caused by male hormones and dihydrotestosterone in hair follicles, dihydrotestosterone will reduce the gold flow of hair follicles and hair dermal papilla, so that scalp raising Insufficient or oxidative reaction, the general hair tonic formula has ingredients that eliminate or alleviate the above symptoms, such as adding the extract of sauerkraut, vitamin E and its derivatives, or using acyl derivatives to increase the blood circulation of the scalp. Or add amino acid such as serine or methionine, vitamins such as B6 and other nourishing hair follicle formula to prevent hair loss and promote hair growth. The US Food and Drug Administration (FDA) proposes two treatments for male baldness: f inasteride (feinasteride, US Patent No. 2006/0099251 A1) and minoxidil (Minodol, US Patent No. 4139619) ; finasteride (finasteride) is a 5 reductase that inhibits activity and converts free testosterone to II201143803 Hydrogen testosterone, triggering hair follicle micronization (Dall〇b, 1994), which was originally used to treat benign prostatic hyperplasia (BPH) ), but later found to be effective in treating male baldness; minoxidil (minoxidil) is a vasodilator 'an original oral drug that can treat high blood pressure (Loniten)' was found in the 1980s Hair growth and inhibition of hair loss; Puqiang has obtained FDA approval for a product containing 2% minoxidil (Minodol) for the treatment of baldness and Rogaine and Regaine, which may be caused by dilating blood vessels and opening potassium channels, resulting in More oxygen, blood and nutrients enter the hair follicle; minoxidil (minoxidil) hair tonic is recommended for use with adenosine (Li '2001' and A2b adenosine as a receptor pathway (lino, 2007) 'exposure that adenosine also has the function of treating baldness' but may have side effects, especially when minoxidil (minoxidil) When used with adenosine, edema, tachycardia, difficulty breathing, fatigue and nausea may occur and may lead to the formation of cancer. Although various methods such as those described above have been attempted, the current hair spray on the market does not necessarily have sufficient ability to prevent hair loss and promote hair growth in any area on the scalp, for example, using finasteride (finasteride) and minoxidil ( When minoxidil is on the top of the head or forehead, almost only the top of the head can effectively produce hair; in addition, most treatments must take a long time to have an effect, such as 4 to 6 months; treatment of baldness This problem can be solved by transplanting hair surgery, but 201143803 will feel pain during the operation and cannot guarantee success, and the hair will even have to wait for months to years to regenerate. Therefore, the above problems will be the difficulties that the technicians in this field want to solve. SUMMARY OF THE INVENTION Therefore, the inventors of the present invention have in view of the above-mentioned prior art, and have proposed a hair growth promoting component which mainly has the following objectives. The main object of the present invention is: hair growth of the present invention. Promoting ingredients, through the right amount of adenine or adenine salts as active substances and cosmetic carriers, can provide raw hair, cleansing lotion, moisturizing lotion, hair care gel, hair styling liquid without harming human skin or scalp Or shampoo, which not only stimulates hair growth, prevents hair loss, but also promotes the growth of eyelashes and eyebrows. [Embodiment] In order to achieve the above objects and effects, a preferred embodiment will be described with reference to the drawings, and those skilled in the art can implement the items for each purpose. Firstly, the present invention provides a hair growth promoting component which mainly contains an appropriate amount of adenine or adenine salt as an active material and a cosmetic carrier. The ratio of the active substance varies depending on the formulation form and the production method. The weight percentage of the preferred fan garden 201143803 is more than 0.0001%, the hair growth promoting component can be in the form of hair lotion, hair lotion, moisturizing lotion, hair care gel, hair styling liquid or shampoo, and is in the form of a liquid, Paste or latex applied to the skin or scalp; in addition, the present invention comprises a cosmetic carrier which may be a diluent, a dispersing agent or a carrier of adenine to promote the distribution of the hair growth promoting component on the skin surface, the carrier The form may be a liquid or solid lubricant, a solvent, a humectant, a thickener or a powder; in addition to the active substance of the present invention and a cosmetic carrier, the hair growth promoting component also contains an additive commonly used in the field of cosmetics, medicine or dermatology. And adjuvants, such as hydrophilic or lipophilic gels, hydrophilic or lipophilic active agents, antiseptic , Antioxidants, solvents, fragrances, fillers, bactericides, deodorants, dyes or colorants, the use of these additives and adjuvants range from 0 total from 01 to 10% by weight of the composition. In order to prove the influence of the adenine or adenine salt content on the hair growth ability in the present invention, the hair growth promoting component of the present invention disposes the aqueous solution of the four samples in a ratio of not proportionally, and the proportion component thereof is not shown in Table 1. The sample configuration process steps include: (1) mixing glandular sulfuric acid with menthol, polyethylene glycol hydrogenated cannabis oil, ethanol, and part of ion-exchanged water, and (4) producing a solution; (7) re-storing the remaining ion-exchanged water It is added to the above solution to form an aqueous solution of hair. 201143803 Spontaneous water configuration sample 1-4 Sample 1 Sample 2 Sample 3 Sample 4 Control group % (w/w)
薄荷醇 0.0001-0.0005 0. ΟθΤ-0. 002 0.05 成分 腺嘌呤硫酸鹽 1. 1.Menthol 0.0001-0.0005 0. ΟθΤ-0. 002 0.05 Ingredients Adenine Sulfate 1. 1.
聚乙二醇-40氫化蓖麻油 0.5 0.5Polyethylene glycol-40 hydrogenated castor oil 0.5 0.5
表一 (a)進行小鼠之生髮試驗 將上述樣本1-4塗抹於毛髮生長循環已靜止之小 _鼠身上以進行試驗，這些小鼠已透過剪刀或剃刀將其背 後之毛髮剃掉，再將樣本1至4之生髮水塗於其背部被 剃掉毛髮之部位，每十隻小鼠為一組，各組每天塗抹一 次份量為0· 2毫升之樣本，經過14和28天後其毛髮再 生之區域比例之平均數值如表二所示。 201143803 生髮水試驗結果 樣本1 樣本2 樣本3 樣本4 控制組 毛髮再生區域比例（％) 14天後 5 18 38 65 2 28天後 33 42 82 98 21 表二 從表二結果可知，加入腺嘌呤硫酸鹽之樣本2、3、 4較樣本1有顯著之效果，可推知本發明之毛髮生長促 進成分對於毛髮生長具有十分卓越的功效。 (b)進行人體之生髮試驗 本發明具有防止落髮以及促進頭髮生長之效果， 將上述配置之樣本3、樣本4與控制組塗抹於60位患 有禿頭之男性的頭皮上，一天兩次，每次linl份量， 連續塗抹四個月後，透過問卷方式進行人體測驗調 查，其問卷測驗結果如表三所示。 8週後 16週後 樣本3 樣本4 控制組 樣本3 樣本4 控制組 (η = 25) (η = 28) (η = 17) (η = 25) (η = 28) (η = 17) 頭髮型態之 各獨立組（％，該測驗組之百分比） 改變 201143803 +2 (改善） 4 (16%) 5 (18%) 0 (0%) 9 (36%) 13 (47%) 0 (0%) +1 (些微改 7 (28%) 13 2 (12%) 10 9 (32%) 1 (6%) 善） (46%) (40%) 0 (無變化） 14 10 8 (47%) 6 (24%) 6 (21%) 8 (47%) (56%) (36%) -1 (些微惡 0 (0%) 0 (0%) 5 (29%) 0 (0%) 0 (0%) 5 (29%) 化） -2 (惡化） 0 (0%) 0 (0%) 2 (12%) 0 (0%) 0 (0%) 3 (18%) 平均分數 0.60 0.82 -0.41 1.12 1.25 -0.59 表三 從表三結果可知，本發明之毛髮生長促進成分含 有腺嘌呤或腺嘌呤鹽類於頭髮生長具有顯著的功效 (C)進行潔髮乳液之配置 其作法以及組成分請參考下列以及表四所示： (c -1)分別將第一階段與第二階段成分分別混合製成 溶液； (c-2)再將上述之溶液混合製成潔髮乳液。 201143803 配置潔髮乳液樣本5 成分 % (w/w) 第一階段 PEG-40 Hydrogenated Castor Oil 0.5 Tween-20 (Polysorbate 20) 0.2 Octoxynol 10 (Triton X-100) 0.1 第二階段 腺嘌呤硫酸鹽 0.02 薄荷醇 0.1 香料 0.2 離子交換水 98.8 表四 (d)進行洗髮水之配置 其作法以及組成分請參考下列以及表五所示： (d-Ι)透過一授拌器將hydroxy cel 1 ul ose (氧化纖維 素）平均分佈於離子交換水中； (d-2)將上述溶液加熱至70°C後，添加乙二胺四乙酸二 鈉和檸檬酸鈉直到完全溶解； (d-3)將上述溶液添加聚季銨鹽7直到完全溶解； (d-4)將上述溶液冷卻至40°C再加入Sodium laurosyl 、 lauric acid amidopropylbetaine 、 201143803Table 1 (a) Performing a hair growth test in mice The above samples 1-4 were applied to a small squirrel whose hair growth cycle was static. The mice were shaved by scissors or a razor, and then the hair was shaved off. Apply the raw water of samples 1 to 4 to the part where the back is shaved, and set a group of ten mice. Each group will apply a sample of 0.2 ml per day, and the hair will be applied after 14 and 28 days. The average value of the proportion of the area to be regenerated is shown in Table 2. 201143803 Spontaneous water test results sample 1 sample 2 sample 3 sample 4 control group hair regeneration area ratio (%) after 14 days 5 18 38 65 2 28 days later 33 42 82 98 21 Table 2 From the results of Table 2, add adenine sulfuric acid The samples 2, 3, and 4 of the salt have a remarkable effect as compared with the sample 1, and it can be inferred that the hair growth promoting component of the present invention has excellent effects on hair growth. (b) Performing the hair growth test of the human body The present invention has the effect of preventing hair loss and promoting hair growth, and the sample 3, the sample 4 and the control group of the above configuration are applied to the scalp of 60 males with baldness twice a day. After the lind application, the human body test was conducted through questionnaires for four months. The results of the questionnaire test are shown in Table 3. After 8 weeks, after 16 weeks, sample 3, sample 4, control group, sample 3, sample 4, control group (η = 25) (η = 28) (η = 17) (η = 25) (η = 28) (η = 17) hair type Independent groups (%, percentage of the test group) Change 201143803 +2 (improvement) 4 (16%) 5 (18%) 0 (0%) 9 (36%) 13 (47%) 0 (0% ) +1 (slightly changed 7 (28%) 13 2 (12%) 10 9 (32%) 1 (6%) good) (46%) (40%) 0 (no change) 14 10 8 (47%) 6 (24%) 6 (21%) 8 (47%) (56%) (36%) -1 (Slightly 0 (0%) 0 (0%) 5 (29%) 0 (0%) 0 ( 0%) 5 (29%)) -2 (deterioration) 0 (0%) 0 (0%) 2 (12%) 0 (0%) 0 (0%) 3 (18%) Average score 0.60 0.82 - 0.41 1.12 1.25 -0.59 Table 3 From the results of Table 3, the hair growth promoting component of the present invention contains adenine or adenine salts and has remarkable effects on hair growth (C). The configuration of the hair lotion and the composition thereof Refer to the following and Table 4: (c -1) separately mix the first stage and the second stage components into a solution; (c-2) mix the above solutions to form a cleansing lotion. 201143803 Configure cleansing lotion sample 5 Ingredient% (w/w) Stage 1 PEG-40 Hydrogenated Castor Oil 0.5 Tween-20 (Polysorbate 20) 0.2 Octoxynol 10 (Triton X-100) 0.1 Second Stage Adenine Sulfate 0.02 Mint Alcohol 0.1 Perfume 0.2 Ion exchange water 98.8 Table 4 (d) Configuration of shampoo. The method and composition of the shampoo are as follows: and (d-Ι) hydroxy cel 1 ul ose (oxidation) through a stirrer (cellulose) is evenly distributed in ion-exchanged water; (d-2) after heating the above solution to 70 ° C, adding disodium edetate and sodium citrate until completely dissolved; (d-3) adding the above solution Polyquaternium 7 until completely dissolved; (d-4) Cool the above solution to 40 ° C and then add Sodium laurosyl, lauric acid amidopropylbetaine, 201143803
Cocamidopropylamine oxide 與 Lauramide MEA 並且加以混合； (d-5)添加尿素、尼泊金丙S旨、腺嗓吟硫酸鹽、薄荷醇 與香料，以產生洗髮水。 配置洗髮水樣本6 成分 % (w/w) Hydroxycellulose 1.0 乙二胺四乙酸二納 0.1 檸檬酸鈉 0.2 聚季銨鹽7 2 Sodium laurosyl 10 1auric acid amidopropylbetaine 8 Cocamidopropylamine oxide 6 Lauramide MEA 6.2 尿素 0.3 尼泊金丙酯 0.2 腺嘌呤硫酸鹽 0.05 薄荷醇 0.1 香料 0.2 離子交換水 65.55 表五 201143803 (e)進行潤髮液之配置 其作法以及組成分如表六所示，將離子交換水與聚 季銨鹽7完全混合，然後添加瓜爾羥丙基三甲基氯化 錄、二曱基copo 1 yo 1、腺嘌吟硫酸鹽和香料以產生潤 髮液。 配置潤髮液樣本7 成分 % (w/w) 聚季銨鹽7 3.0 瓜爾膠羥丙基三甲基氯化銨 0.3 二甲基 copolyol 1.0 腺嘌呤硫酸鹽 0.02 香料 0.5 離子交換水 95.2 表六 (f)進行眉毛與眼睫毛清潔乳液之配置 其作法以及組成分請參考下列以及表七所示： (f-Ι)分別將第一階段與第二階段成分混合製成溶液； (f-2)再將上述之溶液混合製成眉毛與眼睫毛清潔乳 液。 配置眉毛與眼睫毛清潔乳液樣本8與9 樣本8 樣本9 控制組2 12 201143803 成分 % (w/w) 第一階段 PEG-40 Hydrogenated 0.5 0.5 0.5 Castor Oil Tween-20 (Polysorbate 20) 0.1 0.1 0.1 第二階段 腺嘌呤硫酸鹽 0.1 0.05 0 香料 0.2 0.2 0.2 無熱原水 99.0 99.05 99.1 表七 (g)進行眼睫毛清潔乳液之人體測試 本發明之眼睫毛清潔乳液，以下透過問卷方式以 進行人體測驗調查，其測試對象為將上述配置之樣本 8、樣本9與控制組2藉由棉花棒塗抹於48位女性的 眼睫毛上，一天一次，每次0.5ml份量，連續塗抹三 個月後，其問卷測驗結果如表八所示。 施用二個月後 樣本8 樣本9 控制組2 (η = 16) (η = 16) (η = 16) +2 (改善） 2 (12.5%) 4 (25%) 0 (0%) 13 201143803 +1 (些微改善） 8 (50%) 10 (67. 5%) 2 (12.5%) 0 (無變化） 6 (37. 5%) 2 (12.5%) 12 (75%) -1 (些微惡化） 0 (0%) 0 (0%) 2 (12.5%) -2 (惡化） 0 (0%) 0 (0%) 0 (0%) 平均分數 0.75 1.13 0.13 眼睫毛平均生長長度（mm) 0.12 0.22 0 表八 由表八結果可知，本發明之眼睫毛清潔乳液之成 分含有腺嘌呤或腺嘌呤鹽類於眼睫毛生長具有顯著的 功效。 (h)進行眉毛清潔乳液之人體測試 本發明之眉毛清潔乳液，以下透過問卷方式以進 行人體測驗調查，其測試對象為將上述配置之樣本8、 樣本9與控制組2藉由棉花棒塗抹於60位女性的眼睫 毛上，一天一次，每次0.5ml份量，連續塗抹兩個月 後，其問卷測驗結果如表九所示。 施用二個月後 樣本8 樣本9 控制組2 (η = 20) (η = 20) (η = 20) 201143803 +2 (改善） 4 (20%) 3 (15%) 0 (0%) +1 (些微改善） 6 (30%) 10 (50%) 2 (10¾) 〇 (無變化） 10 (50%) 7 (35%) 18 (90%) -1 (些微惡化） 0 (0%) 0 (0%) 0 (0%) _2 (惡化） 0 (0%) 0 (0%) 0 (0%) 平均分數 0.7 0.8 0.1 眉毛平均生長長度（mm) 0.18 0.25 0 表九 由表九結果可知’本發明之眉毛清潔乳液之成分 含有腺嘌呤或腺嘌呤鹽類於眼睫毛生長具有顯著的功 效0 (i)人毛髮真皮乳狀突起細胞再生測試 透過腺嘌呤提升之細胞再生成效可藉由衡量人毛 髮真皮乳狀突起細胞之數量，於下列條件下比較腺嘌 呤核苷與minoxidi 1(米諾地爾）之作用，其結果如圖 一所不；測定毛髮真皮乳狀突起細胞的細胞培養液中 含有的腺嘌呤、腺嘌呤核苷與minoxidil (米諾地爾）， 組成分包含0.002%的腺嘌呤、腺嘌呤核苷與 minoxidil (米諾地爾），其測試方法為： (卜1)配置〇·002%腺嘌呤、腺嘌呤核苷 (米諾地爾）控制測試毛髮真皮乳狀突起細胞之 介質； 15 201143803 (1-2)將人毛髮真皮乳狀突起細胞分別植入％格培養 皿申，其也、度為5000個細胞/格，並且添加〇 之培養液至各格培養皿中； (i-3)於植入細胞12、24、36、48與60小時後，再使 用視覺計數器來計算所有細胞之數量； (1-4)從第一圖之結果可知，腺嘌呤很明顯地可提升人 毛髮真皮乳狀突起細胞之再生能力，推得本發明 之毛髮生長促進成分係具有顯著之護髮效果，可 提升人類或哺乳類之毛髮生長能力並且能防止毛 髮之脫落。 综合上述，以上為本發明之一較佳實施例，非因 此拘限本發明之專利㈣，本案專利範圍仍應以後附 之專利申請範圍所定義為準。 201143803 *' 【圖式簡單說明】 第一圖係本發明之毛髮真皮乳狀突起細胞再生測試比 較圖。 【主要元件符號說明】 無Cocamidopropylamine oxide is mixed with Lauramide MEA; (d-5) Urea, paraben, adenine sulfate, menthol and flavor are added to produce shampoo. Configure shampoo sample 6 Ingredient% (w/w) Hydroxycellulose 1.0 Ethylenediaminetetraacetate 0.1 Sodium citrate 0.2 Polyquaternium 7 2 Sodium laurosyl 10 1auric acid amidopropylbetaine 8 Cocamidopropylamine oxide 6 Lauramide MEA 6.2 Urea 0.3 Nibold Propyl ester 0.2 Adenine sulfate 0.05 Menthol 0.1 Perfume 0.2 Ion exchange water 65.55 Table 5 201143803 (e) Configuration of the hair lotion. The method and composition are shown in Table 6. The ion exchange water and the polyquaternium 7 Completely mixed, then guar hydroxypropyltrimethyl chloride, dimercapto copo 1 yo 1, adenine sulfate and perfume were added to produce a conditioner. Configure Run Condition Sample 7 Ingredient % (w/w) Polyquaternium 7 3.0 Guar Hydroxypropyl Trimethyl Ammonium Chloride 0.3 Dimethyl Copolyol 1.0 Adenine Sulfate 0.02 Fragrance 0.5 Ion Exchange Water 95.2 Table 6 (f) For the configuration of eyebrows and eyelashes cleansing lotion, the method and composition of the eyebrows are as follows: and (f-Ι) respectively, the first stage and the second stage ingredients are mixed into a solution; (f-2) The above solution is then mixed to form an eyebrow and eyelash cleansing lotion. Configure eyebrows and eyelashes cleansing lotion sample 8 and 9 sample 8 sample 9 control group 2 12 201143803 Ingredient % (w/w) First stage PEG-40 Hydrogenated 0.5 0.5 0.5 Castor Oil Tween-20 (Polysorbate 20) 0.1 0.1 0.1 Second Stage adenine sulfate 0.1 0.05 0 Perfume 0.2 0.2 0.2 Non-pyrogenic water 99.0 99.05 99.1 Table 7 (g) Human eye test for eyelash cleansing lotion The eyelash cleansing lotion of the present invention is tested by a questionnaire for human body test. In order to apply the sample 8, sample 9 and control group 2 of the above configuration to the eyelashes of 48 women by cotton swabs, once a day, 0.5 ml portions each time, after three months of continuous application, the questionnaire test results are shown in Table 8. Shown. After 2 months of application, sample 8 sample 9 control group 2 (η = 16) (η = 16) (η = 16) +2 (improvement) 2 (12.5%) 4 (25%) 0 (0%) 13 201143803 + 1 (slightly improved) 8 (50%) 10 (67. 5%) 2 (12.5%) 0 (no change) 6 (37. 5%) 2 (12.5%) 12 (75%) -1 (slightly worse) 0 (0%) 0 (0%) 2 (12.5%) -2 (deterioration) 0 (0%) 0 (0%) 0 (0%) Average score 0.75 1.13 0.13 Average growth length of eyelashes (mm) 0.12 0.22 0 From Table 8, it can be seen that the components of the eyelash cleansing lotion of the present invention contain adenine or adenine salts and have remarkable effects on eyelash growth. (h) Human eye test for eyebrow cleansing lotion The eyebrow cleansing lotion of the present invention is subjected to a human body test by questionnaire. The test object is to apply the sample 8, sample 9 and control group 2 of the above configuration to the control group 2 by cotton swabs. On the eyelashes of 60 women, once a day, 0.5ml each time, after two months of continuous application, the results of the questionnaire test are shown in Table 9. After 2 months of application, sample 8 sample 9 control group 2 (η = 20) (η = 20) (η = 20) 201143803 +2 (improvement) 4 (20%) 3 (15%) 0 (0%) +1 (slightly improved) 6 (30%) 10 (50%) 2 (103⁄4) 〇 (no change) 10 (50%) 7 (35%) 18 (90%) -1 (slightly deteriorated) 0 (0%) 0 (0%) 0 (0%) _2 (deterioration) 0 (0%) 0 (0%) 0 (0%) Average score 0.7 0.8 0.1 Average growth length of eyebrows (mm) 0.18 0.25 0 Table 9 shows the results of Table IX The composition of the eyebrow cleansing lotion of the present invention contains adenine or adenine salts which have significant effects on eyelash growth. (i) Human hair dermal papilla cell regeneration test can be achieved by measuring the effect of cell regeneration by adenine. The number of hair dermal papilla cells was compared between adenosine and minoxidi 1 (minoxidil) under the following conditions. The results are shown in Figure 1. The cell culture medium of hair dermal dermal cells was measured. Containing adenine, adenosine and minoxidil (minoxidil), the composition contains 0.002% adenine, adenosine and minoxidil (minoxidil), the test side For: (1) Configure 〇·002% adenine, adenosine (Minodol) to control the medium of hair dermal protuberance cells; 15 201143803 (1-2) Human hair dermal papilla cells Separately implanted into a % cell culture dish, which also has a degree of 5000 cells/division, and added a culture solution of alfalfa to each Petri dish; (i-3) implanted cells 12, 24, 36, 48 with After 60 hours, the visual counter was used to calculate the number of all cells; (1-4) From the results of the first figure, it is known that adenine can significantly enhance the regeneration ability of human hair dermal papilla cells, and the present invention is derived. The hair growth promoting ingredient has a remarkable hair care effect, which can improve the hair growth ability of humans or mammals and prevent hair loss. In summary, the above is a preferred embodiment of the present invention, and the patent (4) of the present invention is not limited thereby, and the scope of the patent in this case is still subject to the definition of the scope of the patent application attached hereinafter. 201143803 *' [Simple description of the drawings] The first figure is a comparison chart of the hair dermal emulsion cell regeneration test of the present invention. [Main component symbol description] None
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