TW201130978A - Method of isolation of stem cell populations from peripheral blood using sized-based separation (elutriation) - Google Patents

Method of isolation of stem cell populations from peripheral blood using sized-based separation (elutriation) Download PDF

Info

Publication number
TW201130978A
TW201130978A TW099142523A TW99142523A TW201130978A TW 201130978 A TW201130978 A TW 201130978A TW 099142523 A TW099142523 A TW 099142523A TW 99142523 A TW99142523 A TW 99142523A TW 201130978 A TW201130978 A TW 201130978A
Authority
TW
Taiwan
Prior art keywords
cells
flow rate
peripheral blood
target
population
Prior art date
Application number
TW099142523A
Other languages
English (en)
Chinese (zh)
Inventor
Wayne Marasco
Satish Medicetty
Yajuan Jiang
Original Assignee
Neostem Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Neostem Inc filed Critical Neostem Inc
Priority to TW099142523A priority Critical patent/TW201130978A/zh
Publication of TW201130978A publication Critical patent/TW201130978A/zh

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0634—Cells from the blood or the immune system
    • C12N5/0647—Haematopoietic stem cells; Uncommitted or multipotent progenitors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C12N5/0665—Blood-borne mesenchymal stem cells, e.g. from umbilical cord blood

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Hematology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Rheumatology (AREA)
  • Immunology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
TW099142523A 2009-12-04 2010-12-06 Method of isolation of stem cell populations from peripheral blood using sized-based separation (elutriation) TW201130978A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
TW099142523A TW201130978A (en) 2009-12-04 2010-12-06 Method of isolation of stem cell populations from peripheral blood using sized-based separation (elutriation)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US26686009P 2009-12-04 2009-12-04
TW99104517 2010-02-10
PCT/US2010/058974 WO2011069117A1 (fr) 2009-12-04 2010-12-03 Procédé pour isoler des populations de cellules souches du sang périphérique en procédant à une séparation basée sur leur taille (élutriation)
TW099142523A TW201130978A (en) 2009-12-04 2010-12-06 Method of isolation of stem cell populations from peripheral blood using sized-based separation (elutriation)

Publications (1)

Publication Number Publication Date
TW201130978A true TW201130978A (en) 2011-09-16

Family

ID=44115328

Family Applications (1)

Application Number Title Priority Date Filing Date
TW099142523A TW201130978A (en) 2009-12-04 2010-12-06 Method of isolation of stem cell populations from peripheral blood using sized-based separation (elutriation)

Country Status (2)

Country Link
TW (1) TW201130978A (fr)
WO (1) WO2011069117A1 (fr)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20140341863A1 (en) * 2011-11-01 2014-11-20 Neostem, Inc. Adult mesenchymal stem cell (msc) compositions and methods for preparing the same
US10385307B2 (en) 2011-12-21 2019-08-20 Lonza Walkersville, Inc. Scalable process for therapeutic cell concentration and residual clearance
US11312940B2 (en) 2015-08-31 2022-04-26 University Of Louisville Research Foundation, Inc. Progenitor cells and methods for preparing and using the same
EP3423568A4 (fr) 2016-03-04 2019-11-13 University Of Louisville Research Foundation, Inc. Procédés et compositions pour l'expansion ex vivo de très petites cellules souches de type embryonnaire (vsel)
CN109196357A (zh) * 2016-03-07 2019-01-11 日立化学高级疗法解决方案有限责任公司 用于标记和选择活细胞的封闭系统
US20180179490A1 (en) * 2016-12-27 2018-06-28 Miltenyi Biotec Gmbh CELL COMPOSITION DEPLETED FROM TCRab and CD45RA POSITIVE CELLS
AU2018356674A1 (en) * 2017-10-26 2020-05-14 Guy MARTI Method and apparatus for mesenchymal stem cells purification
US20210395693A1 (en) * 2018-10-26 2021-12-23 Guy MARTI Method and apparatus for mesenchymal stem cells isolation and purification
US11697799B2 (en) * 2019-04-15 2023-07-11 Ossium Health, Inc. System and method for extraction and cryopreservation of bone marrow
EP4181675A4 (fr) 2020-07-18 2024-04-24 Ossium Health Inc Perméation de corps vertébraux entiers avec un cryoprotecteur à l'aide d'une diffusion assistée par vide
EP4228406A1 (fr) 2020-10-14 2023-08-23 Ossium Health, Inc. Systèmes et procédés d'extraction et de cryoconservation de moelle osseuse
CN117279650A (zh) 2020-12-18 2023-12-22 奥瑟姆健康公司 细胞治疗方法

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1454135B1 (fr) * 2001-12-05 2009-01-21 CaridianBCT, Inc. Procedes et dispositif pour separer des composants sanguins
EP1795587A1 (fr) * 2005-12-07 2007-06-13 Schuler, Gerold, Prof. Dr. Procédés directs pour la culture de cellules dendritiques sans étape de centrifugation
PL2535403T3 (pl) * 2005-12-08 2019-10-31 Univ Louisville Res Found Inc Bardzo małe komórki macierzyste typu embrionalnego (VSEL) oraz sposoby ich izolowania i zastosowania
US20100095669A1 (en) * 2007-03-16 2010-04-22 Csir Wax Actuator and a Method of Actuating by Means of a Wax Actuator

Also Published As

Publication number Publication date
WO2011069117A1 (fr) 2011-06-09

Similar Documents

Publication Publication Date Title
TW201130978A (en) Method of isolation of stem cell populations from peripheral blood using sized-based separation (elutriation)
Ip et al. Mesenchymal stem cells use integrin β1 not CXC chemokine receptor 4 for myocardial migration and engraftment
Rebelatto et al. Dissimilar differentiation of mesenchymal stem cells from bone marrow, umbilical cord blood, and adipose tissue
Amati et al. Generation of mesenchymal stromal cells from cord blood: evaluation of in vitro quality parameters prior to clinical use
US7585670B2 (en) Automated methods for isolating and using clinically safe adipose derived regenerative cells
WO2011069121A1 (fr) Cellules souches mésenchymateuses (csm) isolées dans le sang périphérique mobilisé
US20160113967A1 (en) Systems and methods for manipulation of regenerative cells from adipose tissue
US20080038231A1 (en) Processing procedure for peripheral blood stem cells
Lan et al. Stromal cell-derived factor-1 mediates cardiac allograft tolerance induced by human endometrial regenerative cell-based therapy
WO2007024441A2 (fr) Compositions cellulaires enrichies destines a la combinaison de diverses populations cellulaires a base de cellules souches et de cellules progenitrices, leurs methodes d'utilisation, et methodes de mise en banque privee
EP2760996B1 (fr) Cellules souches somatiques
WO2006014156A1 (fr) Systemes et procedes pour isoler et utiliser des cellules regeneratives cliniquement sures derivees de tissus adipeux
TW200524647A (en) Tangential flow filtration devices and methods for stem cell enrichment
US20160158292A1 (en) Method and apparatus for recovery of umbilical cord tissue derived regenerative cells and uses thereof
EP1751661B1 (fr) Procede de croissance, de selection et d'enrichissement selectifs de populations de cellules souches/cellules progenitrices
Nasti et al. The CD45+ fraction in murine adipose tissue derived stromal cells harbors immune‐inhibitory inflammatory cells
US11674131B2 (en) Indirect ultrasonic cavitation-derived perivascular cells and methods of use thereof
US20230149523A1 (en) Treatment of autoimmunity and transplant rejection through establishment and/or promotion of tolerogenic processes by fibroblast-mediated reprogramming of antigen presenting cells
Dannull et al. Transfecting Human Monocytes with RNA
Pellegrini Myoblast Transplantation: Murine Studies
Larson Characterization of Stromal Cells Derived from Bone Marrow, Dermis, and Myocardium
Götherström Characterisation of human fetal mesenchymal stem cells
Seshi Method for enhancing engraftment of cells using mesenchymal progenitor cells
JP2004147531A (ja) 細胞処理方法
Duran et al. Research Article Age-Related Yield of Adipose-Derived Stem Cells Bearing the Low-Affinity Nerve Growth Factor Receptor