TW201037308A - Detecting device and detecting method thereof - Google Patents

Abstract

A detecting device of a strip shape for detecting a detected substance of a liquid sample is provided. The detecting apparatus includes an extraction member, an attachment member, a detection member, an absorption member and a support member of fluid impermeability. The extraction member directly extracts a liquid sample from an organism. The attachment member holds a marker reagent specifically combined with the detected material in the state where the marker reagent is movable with the movement of the liquid sample. The detection member fixes a detecting reagent which catches the combination of the detected substance and the marker reagent by combining with the detected material specifically. The absorption member absorbs the liquid sample. The extraction member, the attachment member, the detection member, and the absorption member are arranged in a longitudinal direction of the detecting device on the support member so that the liquid sample may move inside of these members. The extraction member includes a protrusion part, wherein the protrusion part protrudes and projects from the support member at the upstream side of the moving direction of the liquid sample.

Description

201037308 3l001pif VI. Description of the Invention: [Technical Field] The present invention relates to a detecting device and a detecting method. [Prior Art] As a strip detecting device for detecting a detected substance in a liquid sample derived from a living body, for example, the device disclosed in Patent Document No. 3 has been disclosed. Known. In general, for the preparation of the aged material in the liquid sample derived from the raw body, it is necessary to perform a complicated operation through a plurality of steps, including the liquid sample extracted from the living body. After the predetermined treatment of dilution, extraction, etc., the above device is applied. As a device for simplifying the steps, for example, a side device for directly extracting a sample from a living body has been proposed as described in Patent Document 4'. PRIOR ART DOCUMENT Patent Document Patent Document 1: Japanese Patent No. 2,919,392, Patent Document 2: Japanese Patent No. 2,890,384, Patent Document 3: Japanese Patent Laid-Open Publication No. 2003-121445 No. 4: Japanese Patent Publication No. 2005-529305 When pm ^ field directly extracts liquid samples from living organisms, there is a shape due to the shape. In addition, when a large amount of liquid sample is extracted, the detection device must be in contact with the body for a long period of time, and the burden on the living body becomes Bigger. On the other hand, in order to reduce the burden on the living body, it is difficult to obtain a sufficient amount of liquid sample by shortening the contact time of the living body of the 201037308 31001pif measuring device. As a result, the measured value is deviated, and sufficient results cannot be obtained. As described above, when a liquid sample is directly extracted from a living body, there is a problem that the burden on the living body is large, the amount of extraction is small, and the detection sensitivity is low. In this regard, although the material of the portion for extracting the liquid sample has been mentioned in Patent Document 4, the specific constitution for solving these problems has not been sufficiently disclosed. [Invention], therefore, the purpose of the present invention is to provide a detection device and a detection method capable of directly extracting a liquid sample from a living body to obtain sufficient detection results and reducing the burden on the living body. . That is, the present invention is a detecting device which is a strip-shaped gamma device for side-side substances in a liquid sample, the side device comprising: an extracting member directly extracting a liquid sample from the living body; and a holding member, a labeling reagent comprising: a specific binding agent for binding to a test substance, the labeling reagent being held in a state of being movable with the movement of the liquid t; and detecting means, the upper layer is specifically combined with the detected substance a fine (four) agent that captures a combination of a test substance and a labeling reagent, the system has been fixed; absorbs a liquid sample that can be absorbed; and a liquid-impermeable support member; extracts a center of the heart, a member, and a test The member and the absorbing member are arranged in the order of the supporting member, and the extracting member includes the protruding portion=== ^. The upstream side of the moving direction of the liquid sample protrudes from the supporting member and protrudes from the support of 201037308 31001pif In the device, the liquid sample extraction member directly from the living body contains a protruding portion that protrudes from the support member and protrudes. By this, the extraction member is brought into contact with the biological sample extraction unit, and only the dog portion is brought into contact with the portion, thereby avoiding pain of other members such as the support member. As a result, it is possible to sufficiently reduce the pain that the living body feels due to contact with each reagent, and the t to the living body has a flat surface, and the above-mentioned right eight a can be further reduced. The sputum set includes a support member that is impervious to the secret, and the liquid sample extracted by the Detective is leaked from the extraction member, the holding member, and the back side of the cow. The support member for preventing the leakage of the liquid sample from the back surface is preferably disposed on the lower surface of the detection, thereby preventing the backflow of the liquid sample, thereby maintaining the member. A support member is also provided on the downstream side. (4) Perform m ’ = to extract a small amount of liquid sample, or to _ _. The extraction member is brought into contact with the sample extraction site to carry the miscellaneous load & (4)/, and the detection device can directly detect the result from the biological test, and can reduce the production of the device in the living device. A chromatographic detecting device for detecting a liquid sample object, for example, a chromatographic detecting device for a sample of a living body such as a direct self-producer & Weight: = cow, the portion overlapping with a portion of the member, preferably along the length of the length of the holding member in the longitudinal direction of the holding member, more preferably the length direction of the portion The length is longer. Thereby, the contact area of the 201037308 ^ιυυιριι ΖίΓΓ冓 member becomes large, so that the liquid sample is easy to move the holding member toward the detecting member, thereby making it possible to reduce the ancient A when the dry eye patient or the like is born. 'More preferably for these components to turn the components above:: two. Thereby, a capillary flow in the vertical direction is generated in the overlapping portion, so that the liquid sample is more easily moved by the capillary member to the detecting member. That is, a part of the holding member is a part of the measuring member which is partially overlapped. The supporting member having a liquid-impermeable property is preferably placed on the lower surface of the overlapping portion, more preferably s = r5 mm. Thereby, the fiber substrate of the member and (4) member, which is preferably a financial item, can be more effectively used. This is achieved by holding the labeling reagent on the downstream side of the above-mentioned movement of the fibrous substrate. In this way, the single-fiber substrate is formed by the extraction holding member, and the manufacturing process and cost are reduced, and the liquid sample is easy to be: the hair, the, and the field are self-extracting members. Move toward the holding member. In the above-described fiber extraction material and (4) component fiber-side device towel, the fiber substrate has a length of one portion of the component member along the length of the overlapping portion, preferably greater than 2 The length direction of the cross-marking coffee portion along the fiber is preferably longer than the length of the portion along the miscellaneous mark. Thereby, the contact area between the fiber substrate and the side member is such that the liquid sample is easy to capillary phenomenon and the self-weaving substrate is moved toward the Detector 7 201037308 31001pif, and the moving speed of the liquid sample is accelerated, so the debt measurement is performed on the dry eye patient or the like. The burden caused by living organisms is reduced. In this way, the fiber substrate is placed above the detecting member, and the overlapping portion of the C medium generates capillary flow in the up and down direction. Therefore, the liquid sample is more likely to move from the fiber substrate toward the detecting member by capillary action. Preferably, the extraction member or fibrous substrate comprises a paper (four) cloth. Since the water retention per unit weight of the non-woven fabric containing pulp is high, that is, the water-repellent ability is high, even when the liquid sample material is in a small amount, the sensitivity of the extraction of the liquid sample is increased. Further, since the non-woven fabric containing the paper is soft material, it is also preferable in that it is difficult to cause pain when contacting the living body. Further, in the nonwoven fabric containing the pulp, the liquid test j is difficult to spread, and therefore, the above-mentioned nonwoven fabric is used as the extraction member, == degrees. In particular, when a nonwoven fabric comprising a paper-bonded nonwoven fabric and a single-fiber substrate shared by the holding member is used, the lower end portion can be held at the end portion of the downstream side of the fibrous base material. When the sample is directly extracted from the bio-crocohol, the sample is held in the vicinity of the end portion without contacting the living body. Therefore, the nonwoven fabric containing the lining can be preferably used as the above-mentioned fibrous substrate in the detecting device in which the extracting member and the holding member are formed. According to the above advantages, the non-woven fabric containing the paper is suitable as a chromatographic gamma-receiving member which can sufficiently reduce the burden on the living body when the liquid sample is directly extracted from the living body. The nucleus is preferably wood pulp (_d_). Since wood pulp has a particularly high water retention capacity, it is easier to use 木材 ❹ 201037308 iiwipxr to improve the detection sensitivity by using wood pulp. It is also possible to step into the rayon and/or the non-woven fabric containing the pulp. By blending these spiro sounds, ~ or synthetic impurities, the upper side of the material, the casting, the high side of t = the above-mentioned non-woven fabric blended with synthetic weaves == The above-mentioned non-woven fabric is preferably a compressed non-woven fabric non-woven fabric 5 Preferably, the ratio is 4Gmg/emHL=/, the material is equal to 5Gmg/em3, and the thickness is more preferably equal to or greater than 6 coffee, and the thickness of the non-woven fabric is difficult to be less than or equal to G8mm, more preferably less than 0.75mm, more preferably, 丨, Yu Wang # Λ, ^ ^ Yu Yu.65 with. 4; 〇.7_, and more preferably less than, etc. Compressed from a conventional non-woven fabric containing paper, or such a non-woven fabric. W, compressed with a non-woven fabric of compressed pulp containing more than or equal to 1% by weight, so that the thickness is less than 90/. 'The above non-woven fabric is obtained by this. Preferably, the conventionally included woven fabric is compressed by 2 G% or more, i.e., compressed until it reaches a thickness of seven to 80%, whereby the nonwoven fabric is obtained. More preferably, the non-woven fabric containing the paper t is compressed by 3 G% or more, i.e., until the thickness of 7% or less is reached until the nonwoven fabric is obtained. > The maximum width of the extraction member, the holding member, and the detecting member in the direction of the longitudinal direction parent is preferably 0.8 mm to 3 mm. If the width 201037308 31001pif = at 3 mm 'there is a tendency for the liquid sample to be sampled to extract a sufficient amount of liquid sample m = = 7 隹 is present on the _ canister and it is difficult to perform the color development by the labeled reagent 3 The tendency to confirm. Preferably, the seat detecting member further comprises a labeling reagent. The control test is more downstream than the reagent. The pair = ’ 借此 可 ' ' 已 已 已 已 已 已 已 已 已 已 已 已 已 已 已 已 已 已 已 已 已 已 已 已 已 已 。 For the overlapping portion of the detecting member and the absorbing member, either of them may be located above, but the manner 1 in which the absorbing members are located above overlaps each other. When the portion of the member overlaps with the portion of the absorbent member, the liquid-impermeable support member is preferably disposed on the lower portion of the overlapping portion, at least 5 mm from the downstream side of the overlapping portion. More preferably, it is set to at least 10 mm to the downstream side. The length of the above protruding portion is preferably 5 mm or more. If the length of the protruding portion is less than 5 mm, it is possible that when a liquid sample is extracted from the living body, members other than the extracting member of the detecting device become liable to come into contact with the living body, and the age of birth and death is brought about. In particular, when the tear is extracted in the miscellaneous test, when the tear is extracted in the following state, the state is that the protruding portion is inserted into the inferior conjunctival fornix of the living body, and is bent at the outer edge portion of the lower eye examination. The detecting device is allowed to hang down in the wrong direction, and the protruding portion must have a sufficient length. 201037308 31UUlpit on the opposite side of the surface 1 degree ί it mutual separation, can improve the detection of the strong ο 〇 liquid test; ^ self-曰 - this chess bonding member covers the surface of the above components, can prevent touch A smaller amount of extraction is obtained by pressing the first bonding member with the capillary member from above to promote the liquid sample - the above-mentioned crucible is preferably a more adhesive 2 bonding structure #. "2 Adhesive suction and support parts form the grip part. The end of the adhesive member is on the downstream side of the adhesive member. The second adhesive member (four) member, the absorbing member and the second adhesive member can effectively strengthen and detect the components of the adhesive member. 'It's the downstream side of the handheld _ device 201037308 31001pif ^ end and the village does not _ make it difficult to read the hand of the excellent Li 丨 2 'when the hand holds the above hand to use the side device, : The possibility of contacting the user's hand becomes lower, so that it can be safely. . That is, the second bonding member functions as a hand-held portion of the device; the member preferably includes a second support member on the opposite side of the detecting member from the first support 1 supporting body that also serves as the substrate of the detecting member. The nt support member comprises a plurality of support bodies, and the detection device is constructed or volatilized: two = lift: the liquid sample is self-detecting member and the like, and the drain direction is: the first support body is along the length form (the extinction can be Changing the distribution of the second support is obtained by manufacturing = (the length of the detecting device is easily adjusted, so that it can be changed (vanatlon). Further, when the portion other than the support on the 苐1 support is separated, the liquid is at == : 佳 = test __ = 匕 is set to support the support of the structure is better: two: T, the upstream side of the first part at least = stacked, the second support m knife and part of the absorption member weight 12 ο 〇 201037308 ^wvipit is more effective: 〇mm. The fault of this liquid sample is better than f for emphasizing coloration by labeling reagents.

The confirmation of the above-described capture color development on the easy-to-consist component becomes easy: while the detected substance can be easily performed, the support member does not have the function as described above, the opposite side of the support member and the _ member, and the like A background member that emphasizes the function of 3 doses of Z to develop color. It is easy to confirm the color of the capture by having the back, and it is detected by (4) and (4), and the combination of the captured color or the control reagent is captured. Marking the part to develop color and can be landscaped, color, then easy =:: cause color: more: in the case of 'the back of the sticking member - loosely turned paper will show the fixed member as a tape' When the symbol supporting the position of the phase reagent is attached to the background multiple support by the coloring or the like, the dream is on the surface. Also, when the support member contains the measurement U. When the d members are mixed with each other and the strong members are made of paper, it is preferable that the supporting members are square or more than 2 mm as compared with the background. When the part of the support member does not extend toward the upstream side of the background member, or when the ram operation is extended, the liquid sample in the extraction member is saturated to 13 201037308 31001pif the liquid sample is saturated into the background member, The liter or _ device is distorted, and it is difficult to disc color. Further, if the liquid sample is infiltrated from the extraction member to the back, the liquid which moves the holding member or the thin member towel is reduced. Therefore, there is a tendency that the sensitivity is lowered and a sufficient measurement result cannot be obtained. * The weight of the detecting device is preferably less than or equal to G8& if the above weight is greater than 0.8 g', for example, the liquid sample is tear liquid, and the detecting device is self-contained

When the conjunctiva of the organism extracts tears in the direction of the straight line, the 'detection device will fall due to its own weight.

The detection device is particularly useful when the Shengpu is a human. Usually, with the extraction of tears, the burden on the subject is large. In particular, when the subject is suffering from dry eye, there is a tendency to extract only a very small amount of tears of less than 10 after suffering the subject. However, if the above-described detecting device is used, the tear can be extracted without excessive burden on the debt tester, and a sufficient detection result can be obtained even if the tear extraction amount is less than 10 #L. Further, the detecting device is particularly useful when the detected substance is an IgE antibody. In this case, the labeling reagent is a labeling reagent for labeling an antibody having an IgE antibody as an antigen using a labeling substance, and the detecting reagent is an antibody containing an IgE antibody as an antigen, and an antibody having an IgE antibody as an antigen. The reagent is an antibody that uses an antibody possessed by the labeling reagent as an antigen. The IgE antibody in the liquid sample derived from the living body is detected by the above-mentioned detecting means', whereby it is possible to easily determine whether the living body will suffer from allergy such as hay fever such as 14201037308 ^ιυυΐριί. In other respects, the present invention has a method of performing a method of performing a liquid on a liquid sample towel. According to the detection method, the liquid sample can be directly extracted from the living body, and the burden on the living body of the living body can be fully obtained. [Effect of the Invention] The Geno County Invention 'provided a kind of (4) straight 试样 sample to obtain the _ test result of the county, and the side device and method can be reduced. Negative state trr of each object: The above features and advantages can be more clearly understood. The following is a specific example and is described in detail below with reference to the drawings. Further, the following is a detailed description of the best mode as shown in FIG. 4 as follows: However, the repeating m-cut size ratio is the same as: The side end measuring device 1 of the embodiment shown in Fig. 例如, for example, forms a device having a width of about ·5·1亍. Detecting strips (such as strips or short grids with an elongated length of about 57 mm) include an extraction member 10, a holding member of about 0.032 g. Predictive construction (4), support structure (4), 201037308 31001pif detector 20b, and background The member 22, the holding member 〇, the holding member ο, the measuring member 14 and the absorbing member 16 are arranged on the supporting member 18 along the length direction of the detecting device so that the tear liquid moves in the member by capillary action. The extraction member 10 is a member called a "sample pad" for absorbing and holding a liquid sample in the detecting device, and may be exemplified by filter paper, cotton yarn, polyester, glass fiber, or the like as the extraction member 10. The material of the extraction member 10 is preferably a non-woven fabric containing pulp. The term "pulp" refers to a cellulosic fiber extracted by mechanically and/or chemically treating wood or other plants. "Non-woven fabric" refers to a fabric that is formed into a sheet shape without being woven in a fiber but superposed on a fiber in a fixed direction or a random direction. The material, the paper, the film, and the like are different, and a method of heating, a method of winding the fibers together, and a method of using an adhesive, etc., are used as a method of obtaining a non-woven fabric by forming the superposed fibers into a sheet shape. On the other hand, the term "filter paper" is mainly used to filter paper which is different from non-woven fabric. There are cotton fibers of cotton wool (cotton linter) which are seeds in the center of the flower of cotton. The filter paper prepared as a raw material or the filter paper prepared using borsilicate glass as a raw material is characterized by availability (particle retention energy (βΠΙ), initial filtration consistency, and load capacity).遽 ❹ 37 201037308 ^iWipir More water 4 When _ substance is a trace amount, H7 is also convenient for the liquid sample included == two: inclusive _ Non-woven fabric is a soft, temperate liquid sample, which can be used as a burden on the bio-weaving fabric in this respect. Non-woven cloth, the liquid sample is not easy to spread, and the recording of the material will force the t-woven fabric to be used as the extraction member 1〇 to improve the fiber to be detected with the single--; when the extraction member 1〇 and the holding member 12 are common For the non-woven fabric, (4) the above-mentioned pulp containing liquid which is not easily diffused, the liquid is extracted from the living body for the second time: 2: The direct:: two-containing non-woven fabric can be preferably used as the above-mentioned fibrous substrate in the detecting device. In view of the fact that the water retention is higher, the wood pulp which is a raw material for extracting members and materials is used, and it is preferably a wood pulp produced by a mechanical method, and the mechanical method is used as a method for producing pulp. For example, the paper of the coniferous tree = the original family, and the paper group using the hardwood tree as a raw material can be exemplified as the pulp contained in the wood paper extracting member 10. More preferably, it is 80% or more, and more preferably it is large; Kyu et al. == at 17 201037308 31001pif The extraction member 1 can be manufactured according to a known method. For example, an air laid method is used to disperse the fibers of the pulp in the air to form a fiber mat, and the special binder (Wn (4) is opened and connected to make the fiber matte sheet, thereby manufacturing a pulp-containing sheet. $ Extraction member 10. 35 cloth can also be used to blend the enamel and/or synthetic fibers into the non-woven fabric of the above-mentioned package. By blending these enamel and/or synthetic fibers, the upper = paper woven The strength, surface smoothness, and softness of the cloth are improved. In particular, the above-mentioned non-woven fabric blended with the fiber is preferably used in the case of a good water absorption speed. σ "明螺奋" means that the cellulose fibers of the paper are dissolved. A regenerated fiber produced by spinning with sodium hydroxide _ and the like to form a mucus (viscose) and then spinning at the crucible, which is different from synthetic fibers. The so-called "synthetic fiber" means The synthetic polymer obtained by polymerizing the low molecular weight monomer as follows by various spinning methods is made into a fiber, and the 'two low molecular weight monomer is chemically synthesized using petroleum, natural gas or the like as a raw material. The monomer obtained. ° When the fiber mat is formed, the layer is deposited on the two sides or one side of the fiber, thereby producing a non-woven fabric doped with bismuth. Further, when the fiber mat is formed, the synthetic fiber is blended The intermediate layer or the skin layer of the fiber mat can thereby produce a non-woven fabric blended with synthetic fibers. Further, the extracting member 10 is preferably a compressed nonwoven fabric. The liquid test in the detecting device 1 is performed by using the compressed non-woven fabric. The moving speed and the soaking speed are accelerated, and the detection time is shortened. The burden on the living organisms such as dry eye patients is reduced. The density of the non-woven fabric is preferably greater than or equal to 201037308.

JIUUipiI 40 mg/cm3. 3 , 匪. For example, it is preferable that the thickness of the non-woven fabric is not less than or equal to ο. 8 and the compression ratio of the non-woven fabric is less than or equal to 1 G%, and the nonwoven fabric containing the pulp is compressed to have a thickness of 90% or less. The outer portion includes the protruding portion 1()a and the protruding portion 10a to move the side portion 10b, and the protruding portion 10a is on the side of the tear liquid (hereinafter referred to simply as "upstream side";) from Ο ο Π Out = points. The protruding portion (10) is covered with other members such as a member member. Further, the protruding portion is equal to or equal to a flat surface. The length of the protruding portion 10a = use detection device! When extracting human tears, it will highlight the subconjunctival f in the part, and detect the straightening of the coffee Ξ: = extract the tears. At this time, since the protruding portion (10) is composed of a paper nonwoven fabric substrate, it is easy to absorb the tear liquid. Moreover, since the above-mentioned secret material is recorded as a ship, it is not easy to give the subject a second time. Furthermore, since the protruding portion 10a is a button, it is easy to carry out a series of operations, in particular, since the protruding portion 10a has a flat surface and is in contact with the sample extraction portion, the length of the shirt can be further reduced. It is equal to 5: Therefore, the member other than the extraction member 10 is in contact with the eyeball or the like of the subject. Next, the tear fluid absorbed into the extraction member 10 is moved toward the holding member by capillary action. The holding member 12 contains a pulp non-woven fabric or the like, and the 201037308 3100Ipif reagent is a labeling reagent specifically bound by a gold colloid. The ladder body was used as an anti-phase (7) chest (marking substance) to mark the 胪 5 5 5 5 5 5 5. The labeling reagent is capable of being held in the state of lacrimal action in the state of lacrimal movement, and is moved in the holding member 12 and the detecting member 14 to form a combination of the body, from the particle ===: = External, also a special device for latex, etc., and i uses color-developing particles that do not need to be used to confirm the color. A red or blue piece that is easily confirmed by the valley: in the setting 1, the tearing movement direction of the holding member 12 and the extraction structure is the fiber substrate == member 12. When the self-extracting member Η) to the holding structure/12 field tube holding member 12 is not in the above-mentioned manner, in order to maintain the detection of the agricultural device 10 and the maintenance, it is preferable to make the two parts-parts 0 each other. , , = the strength of 1 is set, and the total volume is apt to become large. Regarding this point, "in this case, the combined measurement device 1 =:!Γ volume can be reduced by the -bodyization", so that the detection result can be reduced with less extraction. The wooden piece is full 20 201037308 j ιυυιριι The above-mentioned fiber substrate has the length direction of the material 1:4=, the holding member 12 formed by the weight # part of the holding member 12 is holding the mark test remaining equal to the retention The labeling reagent ====' is preferably greater than the length of the length of the trapping T. The upper square line overlaps the Lai Wei New is located in the _member 14 Ο 的 的 capillary self-裎BlUa night body # easy to hunt The capillary member is moved from the extraction member U) and the holding member 12 toward the detecting member 14. The moving speed of the liquid sample moving toward the lying member is increased, and even if the amount of liquid in the body is small, it can be shorter. The measurement is completed in time, and the burden on the living body such as the dry eye patient is reduced. According to the above, the length of the overlapping portion of the fibrous substrate and the detecting member 14 is better than the portion holding the labeling reagent ( The length of the retaining member 12) is longer: If the length of the overlapping portion is long, the liquid-impermeable supporting member becomes longer or the manufacturing constraints are increased, for example, it is necessary to fix with a longer bonding tape so that the overlapping portion is not easily peeled off. It is preferable that the length of the overlapping portion of the fibrous base material and the detecting structure 14 in the longitudinal direction is preferably 6 mm or less, more preferably 5 mm or less. If the length of the overlapping portion is greater than 5 mm, the volume of the tear-soaked portion is There is a tendency to increase the amount of tears that are difficult to extract for detection. The detecting member 14 includes a nitrocellulose membrane 14c and a detection reagent and a control reagent immobilized on the nitrocellulose membrane 14c. The debt test agent fixing portion 14a on the nitrocellulose membrane 14c is fixed in a line shape orthogonal to the longitudinal direction of the detection 21 201037308 31001 pif device 1. The detection reagent contains an identification site for the IgE antibody and is associated with the IgE antibody. Specifically bound antibody, i the above marker = _ has a steroidal _ 朗 彳贞. 彳贞 test by ί if = specific binding to capture the above IgE antibody and labeled test i's heart. So, by The reagent is applied to the upper reagent, and the portion 14a exhibits a color derived from the labeling substance (^ is red when the gold colloid is used as the labeling substance). The presence of the IgE antibody in the tear is determined by visually confirming the line. In the control reagent fixing portion 14b located on the downstream side of the diamed portion 14a on the nitrocellulose membrane 14c, the straw is fixed in a line shape orthogonal to the longitudinal direction. The control reagent is a labeled reagent. The antibody acts as an antibody to the antigen. The rhyme test captures the tears from the (four), and the medium-labeled reagent is captured, whereby the line derived from the color of the marker substance is revealed at the tear of the fixed portion of the control reagent, and the line can be visually observed. Specifically, it is determined that the tear fluid has moved to the control reagent fixing portion 14b, that is, a sufficient amount of tear liquid for detection. The absorbing member 16 is made of a material that absorbs tears such as cellulose. The absorbing member 16_ is absorbed by the tear fluid and the labeling reagent moved from the price measuring member 14 by the capillary phenomenon. Further, after the tearing liquid is extracted, when the extraction member 10 is immersed in a developing liquid such as pure water to spread the tear liquid, the developing liquid is absorbed by the absorbing member 16 to smoothly spread the developing liquid. That is, the absorbing member 16 has a function of preventing. Further, the absorbing member 16 also has a function of removing the inclusions rinsing from the developing liquid from the detecting member 14. 22 201037308

3iVUipiI support component 18 by poly pair __

Terephthalate, PET, etc. - monoester (P〇lyethylene member Μ comprises the i-supported material. The support structure is preferably a substrate having a length Η with the detecting member 14. "= 造 造 仃 , , , , , , , , 侧 侧 侧 侧 侧 侧 侧 侧 侧 侧 侧 侧 侧 侧 侧 侧 侧 侧 侧 侧 侧 侧 侧 侧 侧 侧 侧 侧 侧 侧 侧 侧 侧 侧 侧 侧 侧 侧 侧 侧 侧 侧 侧 侧The support body is not immersed in the support member. The support body is, for example, a transparent adhesive film made of pET or the like. The second support body is separated into about 5 apart. It is preferable that the length direction is separated, and it is preferable that the view member 22 competes for a change of position. Further, the second support body 18b preferably extends 2 or more than the background member 22 on the upstream side. The tooth is held by the first body. The support body has an adhesive surface, and the debt detecting device 1 is reinforced by the suction member 1〇, the holding member 12, the _ member Μ, and the owing member 16. Since the second tearing up = taking member 1 保持 and holding member 12 In the support body 181, the length direction movement 浸 will penetrate into the second receiving member 16 and 'there is no such thing as the case', tear or The developing liquid leaks from the self-suction portion mi to the second support body (10) and leaks to the outside of the detecting device 1 to the user's hand. Further, 'by the liquid-impermeable material, the second supporting body 18b is larger than the background member 22 Further, the upstream side extends 2 23 201037308 31001ρΐί or 2 mm or more, so that liquid can be prevented from penetrating into the background member 22. Further, since the second support body 18b is separated in the longitudinal direction, the arrangement form of the second support body 18b can be changed. The length of the detecting device 1 can be easily adjusted to obtain a change in manufacturing. Further, when the "18b" is outside the portion overlapping the i-th support 18a, tears or the like may leak to The outside of the device. 脒* The bonding member applies the second bonding member to be formed of, for example, paper. The first bonding member 2A has an adhesive surface, and the bonding member is connected to the extraction member by the bonding. 10, the side of the lion and the side of the 14th training _ part of the i, the opposite side of the surface. In addition, the first Leto contact has a non-adhesive surface on the opposite side of its bonding surface., a test f The member 20a takes the extraction member 10, the holding member 12, and the Detective (4) to prevent these. The pieces are peeled off from each other, thereby improving: Ϊ', the first bonding member 2°a covers the members of the above member, and the outer side of the set 1 has a non-adhesive surface, so when the user is in use, the hand is provided (handle) Part of holding the device into the second bonding member 2〇b star has a twisted two # downstream side of the scale of the square wire wheel contact ^ the faceted 'with the self, the absorption member. and the second support member 14 downstream The end of the side retains the end of the downstream side of the body 18b. Further, 24 201037308 The second bonding member 2% has a non-adhesive surface on the side opposite to the bonding surface, and has a downstream end portion. The grip portion 20b,. The grip portion 20b is formed on the folded portion of the second adhesive member 20b by the second adhesive members 2'b being bonded to each other. The second bonding member 20b bonds the detecting member 14, the absorbing member, and the second supporting member (10), thereby suppressing the separation of the members from each other, thereby improving the strength of the detecting device 1. In particular, the second bonding member 20b can effectively strengthen the structure of the detecting device 1 by bonding the members by nip clamping from the downstream side. Further, the second bonding member is coated with the surface ' of the absorbing member 16 and has a non-adhesive surface on the outer side, thereby having the advantage that the side portion of the downstream side of the hand held copper device 1 can be used without paying the side device. i's user's hand. In particular, when the user holds the hand f portion 2Gb' to use the detecting device 1, it can be used more safely. That is, the second bonding member 18b functions as a hand-held (handle) portion of the detecting device. The view member 22 is disposed on the side opposite to the predicate member 14 of the support member 18. The background member 22 is, for example, a white paper adhesive tape (office seal paper or the like) having a bonding* on the support member 18 side. On the surface of the background member f opposite to the support member 18, a mark indicating the position of the reagent fixing portion 14a and the control reagent fixing portion 14b is attached by coloring. Due to the white background member, the red capture color produced by the gold colloid is emphasized. Further, since the positions of the detection reagent fixing portion 14a and the comparison reagent fixing portion 14b have been shown, it is easy to have a bonding surface for the red 25 201037308 31001 pit view member 22 at each position, so that the liquid and the glazing member 22 are In the case of paper, the liquid crystal or the like, the old view member 22 + may be caused by the fact that the background color is distorted so that it is difficult to capture the capture by the marking reagent; or it is moved to the aged member 14 The amount of tears 2:, staff ^ to get sufficient detection results. In order to make this happen, it is better! The support 18a extends 2 mm or more on the upstream side of the background member 22. Method Packing: Manufacturing of the steps (1) to (7) below r, (1) Holding the labeling agent at the end of the sheet-like pulp nonwoven fabric (forming member 10 and holding member 12). (2) The nitrocellulose membrane 14c is formed on the sheet-like ΡΕτ (first support 18a) by lamination. (3) Applying the detection reagent and the control reagent linearly to the nitrocellulose membrane 14c, and fixing the detection reagent and the control reagent (forming the detecting member 14) 〇 (4) using a transparent adhesive film (No. The support body 18b) bonds the extraction member 1A, the holding member 12, and the first support 18a. Further, the extraction member 10, the holding member 12, and the nitrocellulose membrane 14c are bonded by a paper adhesive tape (first bonding member 20a). (5) Using the transparent adhesive film (the second support 18b), the first member 26 201037308 ^ιυυιριι and the domain member of the Wei component are transferred to the Z-bonding tape (the second bonding member 2 (five) to the first 2 Support = I and nitrocellulose are bonded. (6) Office sealing paper (background member holder 8a, second support (10) and member 2〇ΓΓ width The main embodiment (4) of the 咐 划 划 ' ' 只要 只要 只要 只要 只要 只要 只要 只要 只要 只要 只要 只要 只要 只要 只要 只要 只要 只要 只要 只要 只要 只要 只要 只要 只要 只要 只要 只要 只要 只要 只要 第 第 第 第 第 第 第 第 第 第 第 第 第 第 第 第 第Adjusting the length of the detecting device 1 and extending the structure of the structure: ==1. Also, as shown in Fig. 2, the second connection can be bonded to the background member 22. In this case 2= After the glutinous member 22 is adhered to the second support, the S 2 point joint member is finely bonded. The apparatus of the fourth embodiment H can also be formed as a &quot;body. In the case of the support member ^ = ^, the background member 22 may not be provided. In this case, the function::, ====: color as described above: color is obtained for visual confirmation _ color == color 4 is easy The slidable Hr 'absorbent member 16 may also be lower than the support member 18 or may be as shown in Fig. 6. The extracting member 1 〇 and the retaining member are each composed of different shryling substrates. A part of the extraction member 10 and a portion of the holding member 12 27 201037308 31001pif overlap each other. - and ... IfG, said to be strengthened, capillary phenomenon and the self-extracting member _ holding member 12 is moved by the detecting device 1 Alternatively, the first bonding structure may be provided and the bonding S member may be provided. In this case, the supporting member 18 has a relatively large surface, and the bonding surface is used to apply the members to each other. The present invention will be described more specifically. However, the present invention is not limited to the following embodiments. ..., &lt;A burden on the subject&gt; The tearing liquid is extracted using the detecting device shown in Fig. 1. The detection device is prepared such that the downstream side of the single fiber substrate shared by the extraction member 10 and the holding member 12 overlaps the upstream side of the detecting member 14 by a length of 丨mm, and the amount of 22 /zL/cm is Antibody coated with gold colloid The liquid (OD52 〇 = 8) is applied to the end of the downstream side of the fibrous substrate at a length of about 2.5 mm. When the subject suffers from dry eye, it is necessary to extract enough for detection. The amount of tears (the time until the tears flow out), so it takes a minute or more until the red line of the control reagent fixing portion appears, but the tear can be extracted without causing the subject to feel the burden. If the detecting device shown in FIG. 1 is used, a sufficient amount of tears for detecting can be directly extracted from the detected person without burdening the detected person. &lt;Durability&gt; The above detecting device was placed for one month. As a result, the detecting device maintains the original structure without being twisted or bent. It is thus clear that the detecting device shown in Fig. 1 is sufficiently structurally durable. &lt;Allowable load:&gt; A clip or the like is attached to the above-described detecting device to change the tears. As a result, when the detection load attached to the jig or the like is placed over the entire body load of 0.8 g, it falls down from the conjunctiva of the subject. It is clear from this that the allowable load of the detecting device of this embodiment is less than or equal to 〇·8 _ g. <Water retention capacity of fiber base material> The water retention capacities of the following eight types of fiber base material (A) to fiber base material (H) were tested as follows. (A) Kinocloth KS-40 (Oji Kinocloth): Wood pulp non-woven fabric (B) Palcloth P-40 (Oji Kinocloth company): Wood pulp non-woven fabric blended with enamel (C) Palcloth PB-40P (Oji Kinocloth Co., Ltd.) : Wood pulp non-woven fabric blended with enamel (D) Hi-cloth HAZ-40 (Oji Kinocloth): Wood pulp non-woven fabric blended with synthetic fibers (E) Hi-cl〇th A-40 (Oji Kinocloth Company) ): Wood pulp non-woven fabric blended with synthetic fiber (F) Whatman No.41 Paper (Whatman Japan): Filter paper using cotton fiber as raw material (G) Accuwick Ultra (Pall Japan): Hydroxy polyester 29 201037308 31001pif (hydroxy polyester ) (H) S14 (Whatman Japan Co., Ltd.): Glass fiber The fiber substrate was cut into a square of 2 cm x 2 cm, and the weight (a) of each of the dry fiber substrate sheets was measured. The Qianwei base material sheet was placed in a tray to which 15 mL of ultrapure water was added, and after each of the above-mentioned fiber base materials &gt;} was sufficiently impregnated in ultrapure water for about minute minutes, each The fibrous substrate sheet was lifted up to a parafilm, and the weight (b) of each fibrous substrate sheet in a water-absorbent state was measured. The water retention capacity (water retention capacity I) was evaluated by the following formula. (Formula) (Water retention capacity I) = (b) / (a) In the same manner as above, after each fiber base material sheet has been immersed in an ultra-pure state for 3 minutes, each will be The fibrous substrate sheet was taken up to a metal sieve (32, hole) for dehydration for 1 minute, and then the weight (C) of each of the fibrous substrate sheets was measured. The water retention capacity (water retention capacity II) was evaluated by the following formula. (Formula) (Water retention capacity II) = (c) / (a) The results are shown in Table 1. In Table 1, "ND" means that no real 30 201037308 [Table 1] holding and pushing paper 53⁄43⁄4- quantity (mg) water retention capacity I (e) / (a) i ^) (b) (c) (b) / (a) 727 510 29.9 207^ 22.6 541 438 22.9 UA^~ JAJ_ 564 475 — 21.8 —^ J8.8 433 317 22.0 28.7 776 600 26.0 44.1 219 167 4.0 56.6 3Ϊ4 340 5.8 ~^ 390 ND 10.4 ^ --- Type of fiber substrate • Wood pulp non-woven fabric 右 Right two t not 'wood pulp non-woven fabric (A) ~ wood 羝 feeding l == water capacity 1 is 21·8~ dense, water retention capacity η Π Poly vinegar (6) and The value is relative to the value of 'Wei (F), (5). According to the above results, the woven fabric has a higher water retention capacity than the cotton fiber as the original paper == glass fiber.曰 and its <Fiber substrate retention reagent retention capacity> According to the droplets to be produced by the gold colloid, the labeling agent retention capacity of the fiber substrate (Η) = substrate U 具体 mouth, specifically, the use of micro-tubules (pipetman) bee early position, will be carried out with gold colloid, 々 ― 胁 胁 UL UL UL UL UL UL UL UL UL UL UL UL UL UL UL UL UL UL UL UL UL UL UL UL UL UL UL UL UL UL UL UL UL UL UL UL UL UL UL UL 31 201037308 3100Ipif The use of gold colloidal = (grain control is 4 〇 nm, Tanaka Precious Metals Co., Ltd.) for smaller diameters, and the evaluation of droplets [Table 2] has higher retention capacity. The results are shown in Table 2. Type of fiber substrate

Photograph of the droplets! Pa # #:: 纤论; 基+: 擎·曼, ..虏趣系; As shown in Table 2, the diameter of the droplets in the cloth (8) is relatively small, = (A) ~ wood paper襞 No weaving pain m: For the comparison of the diameters of the droplets in the 'filter paper (7), it is known from the above paste: the wood 'is ^) _. = it is more difficult for the original paper to make the liquid to the mouth &lt;the water absorption speed of the fibrous substrate&gt; according to the water absorption test method as the fiber product

The water absorption rate of ByfeekMeth_'H far fiber substrate U)~(Η) was tested. Fibrous base material 32 201037308 &gt; IWipil First, three fiber base sheets each having a length of 6 cm and a width of 1.7 cm were prepared by cutting each fiber base material, and the marks were used in the longitudinal direction of these fiber base sheets. The ink was scribed at a distance of 〇5 cm from the end. Next, the tray filled with water is prepared, and each of the fiber base sheets is immersed in the water in the tray until the line drawn by the marking ink is used, and the upper end portion of each of the fiber base sheets is fixed to the wall surface of the tray by a tape. Let stand for 3 minutes. Ο— Thereafter, each fiber substrate sheet is taken out from the tray and placed on the paraffin/film, and the vertical distance from the water sucked from the line drawn with the marking ink to the highest point in the vertical direction The maximum distance (the lowest arrival distance) and the vertical distance (the minimum arrival distance) to the lowest point are measured. The higher the price of the above-mentioned southernmost reach, the faster the water absorption rate. The value shown in Table 3 is the average value of each of the three fiber substrate sheets. [Table 3] (A) Fiber substrate Γ---~~-- Type maximum reach (mm) Minimum reach (mm) CB) ... No support and 30.0 21.0 (C) Wood pulp non-woven fabric嫘萦7.7 3.0 CD) 11.0 6.0 (E) Blended with synthetic fibers 32.0 27.3 (F) ------ 32.0 28.0 (6) ------- Paper is greater than or equal to 60 — (H) --- --- greater than or equal to 60 ----- one _ double · 戮 dimension greater than or equal to 60 〇 33 201037308 31001pit B and (C) faster. Furthermore, for the paper (F), the warp group δ曰G a and the glass fiber (H), since the entire fiber substrate sheet = water state ', it is not possible to obtain an accurate value, but with the wood pulp non-woven fabric phase In comparison, the water absorption speed is obviously faster. <Structure of Fibrous Substrate> M. Scanning Electron Microscope for Scanning Electron (This is a review of the top material (a) to the fiber i material (η):: A private electron microscope (SEM) photograph of the substrate (4) to the surface. Maintaining a certain degree of space, one area layer = layer, that is, 'thick fiber, thin glass fiber, but only very small; the ground layer has very good results It is shown that there are several layers of the space-area layer of the burial and the private area. The wood fiber arm is kept to a certain extent, and the diffusion of the body is suppressed. Each unique structure, liquid &lt; debt test time shortening effect&gt; (1) The utility model for shortening the detection time generated by the compressed fiber substrate is prepared to use the following two types as the extraction member _ device 1 of any kind of wood pulp non-woven fabric (7) Then, in each detection device, apply the antibody solution (OD520 201037308 J ιυυιριι = 16) as a labeling reagent. • "Kinocloth KS-40" (Oji Kinocloth): The average thickness is 1.03 mm ( Minimum value 0.85~max I.i5 mm), density is 39.3 mg/cm3 • "KS-40 pressed product": compression ratio is π.9%: average thickness is 0.65 mm (minimum 0.5 to maximum 〇.75 mm), density is 58 9 mg/cm3 . Ο ο Here, the "thickness average" of the above two kinds of wood pulp non-woven fabrics is a non-woven fabric with a width of 17 mm and a length of 25 cm, and a vernier caliper is used for any one. The average value obtained by measuring the thickness of the part is described in parentheses in the minimum and maximum values measured in 1 G. The above-mentioned "compression ratio is calculated by the following calculation formula. Compression ratio (〇/.) = 100 - (" KS_40 pressed" value / "KinGdGthKS_4 ()" thickness average) _"97 thickness is prepared for each of 3 pieces cut into 2 cmx2 cm of woven paper non-woven fabric 'to find each piece of wood / the average weight of the above two kinds of wood "According to (4) of the dry state of the ==, the following calculation formula is used to calculate the above-mentioned thickness average two =;) Γ average weight (-) "two (five) Prepare the following three liquid samples. 1: normal saline

Concentration 0 (IU/mL), )Ik) 'Total IgE 35 201037308 31001pit Sample 2 Following igM is 8.73 (IU/mL) • Sample 3: Total 10% concentration is 34.05 (IU/mL) = Required at the red line = time measurement =: measurement. · Money _ set, and find the average value of the total of 9 times. The amount of the liquid sample was set to 7 Γ = measured, and = 2 was similarly defeated, and the average value was determined. The result ^: [Table 4] The amount of liquid sample (y^L) The average value of the detection time f,

As shown in Table 4, in the case of using a compressed non-woven fabric, that is, a press product, in which uncompressed KM was used, the product was shortened by an average of over or over. In particular, when the liquid of the liquid is hL, the side time is shortened by 65.7%. According to the above sisters, it is clear that the pressure test _, ,, '. =,, the detection time is shortened' to the dry eye 2 and / caused by Wei Wei. When the object is used, it is not confirmed that the red is set to 0 (negative) on the part of the test. When it is confirmed, the line is thinner than the line of the fixing part of the ^36 201037308 f lWipu = agent. In the case of the time, it is judged to be a grade 2 (strong positive) ^rf fixed part of the broken section when the concentration of the concentration of the line is confirmed on the Guding section, and the sample 1 of the sample is G grade (yin ^ In the above-mentioned stage, the sample 3 was grade 2 (strong positive) 2), and it was confirmed that the compressed nonwoven fabric was used. If the detection time is not reduced, the following two detection devices are prepared by shortening the detection by overlapping the fiber substrate and the detecting member. 12 becomes an integrated figure! _2 occupies the &amp; member 10 and the holding and holding structure 2 ===='_1〇 member Μ upstream side of the mt5 1 substrate downstream side and marriage "Kinoc KS_4G" ( 〇jiKinG = ^ overlaps with the same length. The material, y, τ / (company) is used as the above-mentioned fiber base liquid (10)-8 = 'the amount of the antibody labeled with the jinjinlong is dissolved in the downstream of the fiber substrate The end portion of the side is formed to form the holding member 12 having a length of about 3 mm. The following three kinds of liquid samples are prepared. The concentration is the brine (large _ (manufactured)), _ • the sample 2: the total 1 gE concentration 8.37 (IU/mL) • Sample 3: Total 1gE concentration is 34.05 (iu/mL) ^Do not use one of the above five detection devices to perform three detections on one liquid sample with different concentrations of the above-mentioned total city. In the test, the time required to display the red line in the control test 37 201037308 31001pif (_ time) is performed, and each detection device is obtained, and the average value of the detection time of 9 times is determined. Table 5. Dingmen 3 4 5 34.6 1—I27T~ 24.6 [Table 5] Length (mm~T Detection time (seconds)

When the length of the length of the portion where the fiber substrate is overlapped with the workpiece is used, the device with a longer length of the tilt mark _ is compared with the case of the H-degree, that is, the 3 3 mm device. It is clear that the length of the sub-score is smaller than the upper one. It is clear that the inclusion member and the length of the holding member are longer, and it is estimated that even if the amount of the liquid sample is small, it is indicated. : The burden caused by dryness and other patients ^. The _ time is also shortened. When using the visual detection reagent to fix the 〇 grade (negative), when the reagent is detected, the reagent fixing part is judged. The thinner line of the line U is indeed confirmed to be ^ degree =, ' and (we weakly positive when the line of the line can be determined as the level 2; the positive control agent is fixed) In the part-measurement of the Ministry, the sample ί;::. The level of the two fruits (weakly positive), the sample 3 is the second level ((8) raw) _ sample 2 is! Can be confirmed in the fiber substrate and - The overlapped part = degree knot is fruit; = 38 201037308 ^ιυυιρπ ~ 5 mm towel 'the difference of the length does not affect the result = the length of the overlap part is longer than 3 When the detection device of mm is used, it can shorten the detection [industrial availability] The device and method of the present invention can be used for the pollen disease device by the method of making IgE antibodies in humans. The invention can be used for allergies or infections by the antibody contained in the body fluid 2 ^. Although the present invention has been disclosed by way of example, the present invention, Any one of the technical fields has two = non = in order to limit the spirit and scope of the present invention, and when ==, the one that does not deviate from the scope of the patent is attached. 1 is a side end view showing an embodiment of the detecting device of the present invention. FIG. 2 is a side view showing an embodiment of the measuring device according to an embodiment of the present invention. Fig. 4 is a side end view showing an embodiment of the detecting device of the present invention. Fig. 5 is a side end view showing an embodiment of the detecting device of the present invention. Fig. 6 is a view showing the detecting of the present invention. Side end of the measuring device - embodiment

[Explanation of main component symbols] 1 : Reconnaissance device 1〇: extraction member 1〇a • • protruding portion 10b: non-protruding portion 12. Holding member 14: detecting member 14a • detecting reagent fixing portion 14b • fixing reagent fixing Portion 14c: nitrocellulose membrane 16: absorption member 18: support member 18a: first support 18b: second support 201037308 JXV/V/1^/Xi. 20a: first adhesive member 20b: second bonding Member 20b': grip portion 22: background member

Claims (1)

201037308 Jiuuipu VII. Patent application scope: ^ 1. A detecting device, which is a strip detecting device for detecting a detected substance in a liquid sample, the detecting device comprising: an extracting member directly from The living body extracts the liquid sample; the holding member includes a labeling reagent that specifically binds to the detected substance, and the labeling reagent is held in a state of being movable together with the movement of the liquid sample; a side-containing reagent, which is obtained by capturing a combination of the above-mentioned substance and the above-mentioned labeling reagent, which has been fixed; an absorption member capable of absorbing the liquid sample; and a liquid-impermeable property a supporting member; the absorbing member, the holding member, the detecting member, and the holding member are arranged in a length (4) i of the upper paste device, and the liquid sample is sequentially extended from the support member by the capillary phenomenon Out and stand out. (4) 搂ί ^ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ The extraction member and the holding member share a single 42 Ο Ο 201037308 The labeling reagent is held at the end of the side of the fiber substrate in the moving direction to form the holding member. According to the debt measuring device of the second or third aspect of the patent application, the length of the stack portion in the longitudinal direction is longer than the length along the length of the portion of the retaining member in the retaining member. . The above-mentioned ribbing member is a non-woven fabric containing pulp, as described in the first or second item. And the apparatus according to Item 3 or 4, wherein the upper Qianwei substrate comprises a non-woven fabric made of paper. The apparatus described in item 5 or item 6 of Shangzhongwei Guanyi, 〃T The above pulp is wood pulp. For example, the application of the 1st to the third patent to the right side of the 5th to the 7th cup into #, +, the upper test ~ which is in the above-mentioned non-woven 4 =:: 任--- In the _ item, the «10 4 woven fabric is blended with synthetic fibers. Measured cloth: Ren (4) Describe, in the above, the debt testing device, / the compressed non-woven i as described in any of items 10 to 12. &quot;&quot; Non-woven fabric is a compression ratio of greater than or equal to the brain by 43 201037308 3ΐυυιριι 14. As claimed in the scope of claim 1 to the a detection device, wherein the density of the non-woven fabric is greater than: &amp; For example, in the patent scope 2 detecting device, the compressed non-woven fabric described in the thickness # of the non-woven fabric. The above non-woven fabric is more than or equal to chat. The compression ratio is as described in the ninth aspect of the invention, wherein the above-mentioned extraction structure = the member of any one of the lengthwise directions and the above-mentioned _ mm to 3 mm. The width of the ridge in the direction is 0.8 18. As in the scope of the application of the Scope to _ device, wherein the above-mentioned fairy component further comprises a plaque: two: said further downstream side.视,,, she is scheduled to be more than the above detection reagents 19. If the scope of the patent application range i to the side of the section cut 2 ^. The first bonding member connected to the downstream side of the extraction member is adhered to the upstream end of the detecting member, and the opposite side surface of the detecting member And the first! The opposite side of the bonding surface of the supporting member has a _ junction surface to be read on the surface 21. The method of measuring a device according to any one of claims 1 to 20, 2010 2010 308) ιυυιριι, measuring device, further includes a second adhesive member, wherein the second adhesive layer sandwiches the downstream end portion of the braiding member and the downstream end portion of the absorbent structure; and the member is opposite to the member; The ϊ1 of the bottom of any one of the first to the twenty-first aspects of the affixing surface of the above-mentioned respective members is the lining of the bottom supporting member, and the supporting member includes a lining serving as the detecting member and is disposed on the first The second support of the support opposite to the detecting member. The detecting device according to claim 22, wherein the first support weight is the same as the above-mentioned first support weight, and the long (four) = claim 1 of the patent range 1 to 23 The color support member has a function of emphasizing color development by the above-described labeling reagent. The detecting device according to any one of the preceding claims, wherein the detecting member is opposite to the detecting member on the side opposite to the detecting member, and further comprising a pair of the marking reagent A background component that captures the function of color rendering to emphasize. The detecting device according to the invention, wherein the background member is a paper-bonding tape having a difficult surface to the reading member (4), and the supporting member extends 2 mm upstream from the background member. Or 2 mm or more. 27. The device of claim 4, wherein the weight is less than or equal to 8.8 g, as described in any one of claims 1 to 26. The detection of the agricultural product according to any one of the items 1 to 27, wherein the agent according to any one of item 28 is obtained by the labeling substance, and the above label is recorded. For the reagent, the antibody as the antigen is labeled as a different identification site, and the control reagent contained in the labeling reagent is an antibody to which the gE antibody is an antigen, the above-described steroid. (4) The antibody against the 34-label test is used as an anti-antigen for the antigen. 〃, 33 device (four) liquid sample towel is tested i 46