CN206772984U - A kind of multi objective colloidal gold kit for acute injury of kidney Quantitative detection - Google Patents
A kind of multi objective colloidal gold kit for acute injury of kidney Quantitative detection Download PDFInfo
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- CN206772984U CN206772984U CN201720687739.3U CN201720687739U CN206772984U CN 206772984 U CN206772984 U CN 206772984U CN 201720687739 U CN201720687739 U CN 201720687739U CN 206772984 U CN206772984 U CN 206772984U
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Abstract
It the utility model is related to a kind of multi objective colloidal gold kit for acute injury of kidney Quantitative detection, it includes detection card, colorimetric card and/or collaurum quantitative readout instrument, detection card includes loading pad, gold conjugation pad, carrier film, sample suction pad and the bottom plate with detection line and nature controlling line, index includes kidney injury molecule-1, liver type fatty acid binding protein, gold conjugation pad is included with reference to pad body and coated on the colloidal gold labeled monoclonal antibody coating combined on pad body, and the wherein antibody in colloidal gold labeled monoclonal antibody coating is the antibody of the index；The utility model kit includes the bigeminy detection card of quick detection renal failure, it uses two kinds of Kim 1, L FABP labels, can effectively predict renal failure risk, belong to pioneering in terms of the research and development of diagnostic reagent, the kit diagnostic sensitivity is high, high specificity, and the accuracy rate of clinical monitoring is high.
Description
Technical field
It the utility model is related to biotechnology diagnostic field, and in particular to one kind is used for acute injury of kidney Quantitative detection
Multi objective colloidal gold kit.
Background technology
Acute injury of kidney（AKI）It is a kind of common clinical syndrome, is mainly shown as rapid decrease and the generation of renal function
Thank to the accumulation of waste, its traditional diagnosis depends on serum creatinine（Scr）Rise and urine volume reduction, but these indexs exist
Specificity is low, influences the defects of factor of testing result is more.The AKI incidence of disease is high, and in ascendant trend year by year, in inpatient
Especially very common in patient with severe symptoms, severe AKI patient case fatality rate is high.
The I type transmembrane protein that Kim1 (Kim-1) is made up of 334 amino acid residues, in normal kidney group
Hardly expressed in knitting, be in high expression status in people's proximal tubular epithelial cells after ischemic and injury of kidney occur for kidney.Kidney
1st day KIM-1 raises at least more than 5 times after tubule is impaired, and serum creatinine and urea nitrogen the 3rd talent to after being damaged raise, because
This Kim-1 is one of most sensitive mark for monitoring early stage renal damage.The extracellular ectodomain of Kim-1 molecules
(metalloprotein enzyme hydrolysis takes off, and can be secreted into urine) is acute renal failure (AKI) quantitative marker, in urine
KIM-1 detection is not influenceed by urine physics and chemistry material, after openheart surgery several hours will detect that Kim-1 is big in urine
Width increases phenomenon.Kim-1 horizontal rises are urinated during renal ischemic, there is higher specificity, for AKI patient, high level
The prognosis of Kim-1 indication patients is poor.Clinical experiments have proved that Kim-1 is one of most sensitive mark of early stage renal damage.
Liver type fatty acid binding protein (L-FABP) is the important member in fatty acid binding protein superfamily, major function
It is absorption of the body to aliphatic acid, transhipment and the transhipment of intracellular long chain fatty acids, and aliphatic acid dividing again in organelle
Cloth utilizes.L-FABP and injury of kidney L-FABP is expressed in people's renal proximal renal tubular cell.A variety of causes causes acute kidney to be damaged
When hindering, such as ischemia/reperfusion, contrast agent Associated Acute injury of kidney, septicemia and infectious shock, in renal tubular cell significantly
Active oxygen and lipid peroxidation product can cause intracellular ATP exhaustion and oxidation caused by elevated free fatty and its oxidation
Stress, tubulointerstitial injury is aggravated, changes renal tubular cell membrane permeability and promotes L-FABP quickly to overflow.In acute injury of kidney
Found in animal model and clinical case research, 1h urinates L-FABP rises after nephridial tissue ischemic, surveys in early detection and accurately
Determine acute tubular necrosis aspect and have more advantage than traditional renal function mark urea nitrogen.
The urine L-FABP of contrast agent Associated Acute injury of kidney is more more sensitive than serum creatinine, helps doctor to find and monitor injection and makes
The patient of existing renal damage before shadow agent.Show that the biomarker of prediction albuminuria can be turned into by urinating L-FABP.
The content of the invention
Technical problem to be solved in the utility model is the defects of overcoming conventional diagnostic, there is provided a kind of high specific, height
The multi objective colloidal gold kit for acute injury of kidney Quantitative detection of accuracy rate, it can improve early stage AKI diagnosis
Accuracy and specificity, solve the defects of existing clinical detection.In addition, this kind detection card is suitable to whole blood sample and urine sample, can be
The applications such as the simple and crude community sanitary institute of family, home for destitute, condition.
To solve above technical problem, the utility model adopts the following technical scheme that：
A kind of multi objective colloidal gold kit for acute injury of kidney Quantitative detection, it includes detection card, colorimetric
Card and/or collaurum quantitative readout instrument, detection card include loading pad, gold conjugation pad, holding with detection line and nature controlling line
Film carrier, sample suction pad and bottom plate, index include Kim1, liver type fatty acid binding protein, and gold conjugation pad includes knot
Close pad body and coated on the colloidal gold labeled monoclonal antibody coating combined on pad body, it is anti-wherein in colloidal gold labeled monoclonal antibody coating
Body is the antibody of the index；Detection line on carrier film has two, this two detection lines be separated from each other and be respectively can be with
The coating that the pairing antibody or antigen that the antibody specificity of colloid gold label combines are formed.
According to a specific aspect of the present utility model, detection card is by loading pad, gold conjugation pad, carrier film and inhales sample
Interlaced be pasted onto on bottom plate is formed pad successively.
According to a preferred aspect of the present utility model, detection card also includes hemofiltration film or urine filtering film, hemofiltration film or
Urine filtering film is covered on loading pad, and loading pad, gold conjugation pad, carrier film and sample suction pad are interlaced successively to be pasted onto
On bottom plate.
According to another preferred aspect of the present utility model, detection card also includes boosting pad, and detects card by loading pad, colloid
Interlaced be pasted onto on bottom plate is formed successively for golden pad, boosting pad, carrier film and sample suction pad.
According to an also preferred aspect of the present utility model, detection card also includes hemofiltration film or urine filtering film and boosting pad,
Hemofiltration film or urine filtering film are covered on loading pad, loading pad, gold conjugation pad, boosting pad, carrier film and sample suction pad according to
It is secondary interlaced to be pasted onto on bottom plate.
Preferably, the material of boosting pad is glass fibre element film or non-woven fabrics.
According to a preferred aspect of the present utility model, carrier film only has one, and two detection lines, nature controlling lines are parallel to each other
And it is successively set on along the length direction of carrier film on carrier film.
Further, the detection line of close gold conjugation pad is the pairing antibody of Kim1 in two detection lines
Or antigen coating, close to sample suction pad detection line be liver type fatty acid binding protein pairing antibody or antigen coating, nature controlling line
Positioned at the end of the close sample suction pad of carrier film.
According to another preferred aspect of the present utility model, carrier film divides film including two carryings, and two detection lines are divided respectively
Cloth divides on film in two carryings.Divide in each carrying and nature controlling line is respectively arranged with film.
According to another preferred aspect of the present utility model, detection card includes two independent detections point card, loading pad, combines
Pad, sample suction pad and bottom plate have two respectively, and each loading pad, pad, carrying divide film, sample suction pad and bottom plate to be assembled into one
Detection point card.
Preferably, nature controlling line includes but is not limited to the shapes such as sheep anti-mouse igg, goat-anti chicken IgY or goat anti-rabbit igg by coating
Into.
Preferably, carrier film is nitrocellulose filter and is 5~12um of aperture porous spline structure film；Loading pad, colloid
The material of golden pad is glass fibre element film or non-woven fabrics, and the material of sample suction pad is absorbent filter.
Immune colloidal gold technique is known.Colloidal gold immunolabeling antibody described in the utility model can use this area
It is prepared by well known method.
Due to the implementation of above technical scheme, the utility model has the following advantages that compared with prior art：
The utility model kit includes the bigeminy detection card of quick detection renal failure, and it uses bis- kinds of marks of Kim-1, L-FABP
Remember thing, can effectively predict renal failure risk, belong to pioneering in terms of the research and development of diagnostic reagent, kit diagnostic sensitivity height,
High specificity, the accuracy rate of clinical monitoring are up to more than 95%, realize self detection and monitoring of acute injury of kidney, carry out early stage
Early warning can prevent patient in time, and sb.'s illness took a turn for the worse.Reduction is failed to pinpoint a disease in diagnosis caused by detecting not in time, mistaken diagnosis, reduces the death rate.
Further, the utility model detection card will can be only applied to the kit of hospital laboratory originally, by technology
Innovation, by increasing hemofiltration film or urine filtering film, allow its conveniently, it is simple, accurately applied to acute injury of kidney from
I detects and monitoring, suitable for the simple and crude community sanitary institute of family, home for destitute, condition, its application method be also only by patient or
Family members' acupuncture one is bled or directly realizes that self detection of acute injury of kidney and monitoring, mitigation go to hospital to register, arrange with urine
Team, chemically examine, take the complicated processes such as report, mitigating the pressure of public medical mechanism, be relatively beneficial to the daily treatment of patient.
Further, the utility model adds boosting pad in structure, can effectively remove ambient interferences, so as to enter
One step improves diagnostic sensitivity height and the degree of accuracy, and boosting pad additionally aids quickening detection sample flow in addition, improves detection efficiency,
Shorten detection time, colloid gold particle can be avoided to be retained by PVC bottom plates, enhanced product performance.
Brief description of the drawings
Fig. 1 is the main structure diagram blocked according to the detection of the utility model embodiment 1；
Fig. 2 is the dimensional structure diagram blocked according to the detection of the utility model embodiment 2；
Fig. 3 is the main structure diagram according to the detection point card of the utility model embodiment 3（Detection line containing T1）；
Fig. 4 is the main structure diagram according to the detection point card of the utility model embodiment 3（Detection line containing T2）；
Wherein：1,1 ', loading pad；2,2 ', gold conjugation pad；20,20 ', with reference to pad body；21,21 ', collaurum mark
Remember antibody coating；3rd, carrier film；30,30 ', carrying divides film；4,4 ', sample suction pad；5,5 ', bottom plate；6,6 ', hemofiltration film or urine mistake
Filter membrane；7,7 ', boosting pad；T1, T2, detection line；C, C ', nature controlling line.
Embodiment
Embodiment 1
As shown in figure 1, a kind of multi objective colloidal gold kit for acute injury of kidney Quantitative detection, it includes inspection
Survey card, colorimetric card and/or collaurum quantitative readout instrument, detection card includes loading pad 1, gold conjugation pad 2, with detection line and
Nature controlling line C carrier film 3, sample suction pad 4, bottom plate 5, hemofiltration film or urine filtering film 6 and boosting pad 7, hemofiltration film or urine filtering
Film 6 is covered on loading pad 1, and loading pad 1, gold conjugation pad 2, boosting pad 7, carrier film 3 and sample suction pad 4 are interlaced successively
It is pasted onto on bottom plate 5.Wherein, the material of boosting pad 7 is glass fibre element film or non-woven fabrics.Carrier film 3 is nitrocellulose filter
And it is 5~12um of aperture porous spline structure film；Loading pad 1, the material with reference to pad body 20 be respectively glass fibre element film or
Non-woven fabrics, the material of sample suction pad 4 is absorbent filter.
Further, index includes Kim1, liver type fatty acid binding protein, and gold conjugation pad 2 includes knot
Close pad body 20 and coated on the colloidal gold labeled monoclonal antibody coating 21 combined on pad body 20, wherein colloidal gold labeled monoclonal antibody coating
Antibody in 21 is the antibody of the index；Detection line has two, and this two detection line T1s, T2 are separated from each other and respectively can
The coating that the pairing antibody or antigen combined with the antibody specificity of colloid gold label is formed.Two detection line T1s, T2, nature controlling lines
C is parallel to each other and set gradually along the length direction of carrier film 3.Close to gold conjugation pad 2 in two detection line T1s, T2
Detection line T1 is the pairing antibody or antigen coating of Kim1, and the detection line T2 close to sample suction pad 4 is liver type aliphatic acid
Protein-bonded pairing antibody or antigen coating, nature controlling line C are located at the end of the close sample suction pad 4 of carrier film 3.Nature controlling line C leads to
Cross coating and include but is not limited to the formation such as sheep anti-mouse igg, goat-anti chicken IgY or goat anti-rabbit igg.
Each part of this example is so assemblied together, and in hothouse, 20~25 DEG C of temperature, humidity is less than 30%, takes
Bottom plate 5, the middle part that coated carrier film 3 is placed on to bottom plate 5 are pasted, and gold conjugation pad 2 is cut into suitable width,
Detection line side overlap joint boosting pad 7 on carrier film 3, gold conjugation pad 2 is pasted in the opposite side overlap joint of boosting pad 7,
The opposite side of gold conjugation pad 2 overlap joint pastes loading pad 1, and hemofiltration film or urine special filtering film 6 are covered on loading pad 1,
Nature controlling line side overlap joint sample suction pad 4 on carrier film 3, finally will post plastic plate with cutter and is cut into the wide test paper of 3~5mm
Bar, it is reloaded into plastic clip, forms detection card.
The detection method of this example kit is as follows：
（1）Detection reagent and sample are balanced to room temperature, detection card is taken out, keeps flat；
（2）Serum, blood plasma or whole blood sample are drawn, is added in the centrifuge tube of cleaning, is diluted, filled with Sample dilution
Divide and mix；
（3）Sample after drawing dilution with liquid-transfering gun is added in the sample aperture of loading pad, is determined in 15~20 minutes
Property analysis or the concentration according to the standard curve being set in advance in collaurum quantitative analysis detector to Kim-1, L-FABP
Carry out that result is calculated and be shown.
Reagent card is arranged in pairs or groups with collaurum quantitative readout instrument, you can is formed pre- for the early stage of postoperative or daily acute injury of kidney
It is alert.
The Cleaning Principle of this example detection card is as follows：
After sample is added to sample well, Kim-1, L-FABP antigen and the Kim-1 of colloid gold label that contain in sample,
L-FABP antibody bindings, by capillarity, the antibody-antigen immune compound formed along nitrocellulose filter chromatograph to
Detection line, then Kim-1, L-FABP antibody binding with being solidificated in detection line.Uncombined immune complex then continues layer
Analysis is captured to nature controlling line by sheep anti-mouse igg antibody.
After reaction terminates, it can be qualitatively judged according to the colored depth of color, according to being set in advance in collaurum
Standard curve in quantitative analysis detector to Kim-1, L-FABP concentration carries out that result is calculated and be shown.
Embodiment 2
As shown in Fig. 2 this example provides a kind of multi objective colloidal gold kit for acute injury of kidney Quantitative detection,
Its substantially with embodiment 1, unlike, its carrier film divides film 30,30 ' including two carryings being arranged side by side, and each carrying divides
A detection line and nature controlling line are formed on film 30,30 ' respectively.
Preparation, detection and the Cleaning Principle of this example kit are the same as embodiment 1.
Embodiment 3
As shown in Figure 3, Figure 4, this example provides a kind of multi objective collaurum for acute injury of kidney Quantitative detection and tried
Agent box, its substantially with embodiment 1, unlike, detection card includes two independent detections point and blocked, hemofiltration film or urine filtering film
6,6 ', loading pad 1,1 ', pad 2,2 ', boosting pad 7,7 ', carrying divide film 30,30 ', sample suction pad 4,4 ' and bottom plate 5,5 ' respectively
There are two, each hemofiltration film or urine filtering film 6,6 ', loading pad 1,1 ', pad 2,2 ', boosting pad 7,7 ', carrying divide film
30,30 ', sample suction pad 4,4 ' and bottom plate 5,5 ' are assembled into a detection point card, and each carrying on detection point card divides film 30,30 '
It is upper to form a detection line and nature controlling line respectively.
Preparation, detection and the Cleaning Principle of this example kit are the same as embodiment 1.
To sum up, the bigeminy detection card of the utility model kit including quick detection acute injury of kidney, its use Kim-1,
Bis- kinds of labels of L-FABP, can effectively predict renal failure risk, belong to pioneering in terms of the research and development of diagnostic reagent, the kit is examined
Disconnected high sensitivity, high specificity, the accuracy rate of clinical monitoring is high, realizes self detection and monitoring of acute injury of kidney, carries out early
Phase early warning can prevent patient in time, and sb.'s illness took a turn for the worse, and the accuracy rate of clinical monitoring is up to more than 95%.
The utility model detection card passes through technological innovation, by increasing hemofiltration film or urine filtering film, facilitates it
Bled by patient or family members' acupuncture one on ground（Or directly use urine）To realize the monitoring to acute injury of kidney, mitigation goes to hospital to hang
Number, be lined up, chemically examine, taking the complicated processes such as report, mitigate the pressure of public medical mechanism, suitable for family, home for destitute, condition letter
Plain community sanitary institute, patient is relatively beneficial to by daily monitoring, prevents the generation of pernicious renal failure.
The utility model is described in detail above, its object is to allow the personage for being familiar with this art can be much of that
Solve content of the present utility model and be carried out, the scope of protection of the utility model can not be limited with this, it is all according to this practicality
The equivalent change or modification that new Spirit Essence is made, it should all cover in the scope of protection of the utility model.
Claims (10)
1. a kind of multi objective colloidal gold kit for acute injury of kidney Quantitative detection, it includes detection card, colorimetric card
And/or collaurum quantitative readout instrument, the detection card include loading pad（1）, gold conjugation pad（2）, there is detection line and matter
Control line（C）Carrier film（3）, sample suction pad（4）And bottom plate（5）, it is characterised in that：The index includes Kim1, liver
Type fatty acid binding protein, the gold conjugation pad（2）Including with reference to pad body（20）With coated on the combination pad body
（20）On colloidal gold labeled monoclonal antibody coating（21）, wherein the colloidal gold labeled monoclonal antibody coating（21）In antibody be the finger
Target antibody；The detection line has two, this two detection lines（T1、T2）Be separated from each other and be respectively can be with the collaurum
The coating that the pairing antibody or antigen that the antibody specificity of mark combines are formed.
2. multi objective colloidal gold kit according to claim 1, it is characterised in that：The detection card also includes hemofiltration film
Or urine filtering film（6）, the hemofiltration film or urine filtering film（6）It is covered in described loading pad（1）On, the loading pad
（1）, gold conjugation pad（2）, carrier film（3）And sample suction pad（4）It is interlaced successively to be pasted onto bottom plate（5）On.
3. multi objective colloidal gold kit according to claim 1, it is characterised in that：The detection card also includes boosting pad
（7）, and the detection card is by the loading pad（1）, gold conjugation pad（2）, boosting pad（7）, carrier film（3）And sample suction pad
（4）It is interlaced successively to be pasted onto bottom plate（5）Upper composition.
4. multi objective colloidal gold kit according to claim 1, it is characterised in that：The detection card also includes hemofiltration film
Or urine filtering film（6）With boosting pad（7）, the hemofiltration film or urine filtering film（6）It is covered in described loading pad（1）On,
The loading pad（1）, gold conjugation pad（2）, boosting pad（7）, carrier film（3）And sample suction pad（4）Interlaced stickup successively
In bottom plate（5）On.
5. the multi objective colloidal gold kit according to claim 3 or 4, it is characterised in that：The boosting pad（7）Material
For glass fibre element film or non-woven fabrics.
6. multi objective colloidal gold kit according to claim 1, it is characterised in that：Two detection lines（T1、T2）、
Nature controlling line（C）It is parallel to each other and along the carrier film（3）Length direction set gradually.
7. multi objective colloidal gold kit according to claim 6, it is characterised in that：Two detection lines（T1、T2）
In close to gold conjugation pad（2）Detection line（T1）It is close to inhale sample for the pairing antibody or antigen coating of Kim1
Pad（4）Detection line（T2）For the pairing antibody or antigen coating of liver type fatty acid binding protein, the nature controlling line（C）Positioned at institute
State carrier film（3）The close sample suction pad（4）End.
8. multi objective colloidal gold kit according to claim 1, it is characterised in that：The carrier film（3）Including two
Carrying divides film（30,30 '）, two detection lines are respectively distributed to two carryings and divide film（30,30 '）On.
9. multi objective colloidal gold kit according to claim 8, it is characterised in that：The detection card includes two independences
Detection point card, the loading pad（1,1 '）, pad（2,2 '）, sample suction pad（4,4 '）And bottom plate（5,5 '）There are two respectively,
Each loading pad（1,1 '）, pad（2,2 '）, carrying divide film（30,30 '）, sample suction pad（4,4 '）And bottom plate（5,5 '）Assembling
Into the detection described in one point card.
10. multi objective colloidal gold kit according to claim 1, it is characterised in that：The nature controlling line（C）Pass through coating
Sheep anti-mouse igg, goat-anti chicken IgY or goat anti-rabbit igg are formed, the carrier film（3）For nitrocellulose filter and it is 5~12um of aperture
Porous spline structure film；The loading pad（1）, with reference to pad body（20）Material be respectively glass fibre element film or non-woven fabrics,
The sample suction pad（4）Material be absorbent filter.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201720687739.3U CN206772984U (en) | 2017-06-14 | 2017-06-14 | A kind of multi objective colloidal gold kit for acute injury of kidney Quantitative detection |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201720687739.3U CN206772984U (en) | 2017-06-14 | 2017-06-14 | A kind of multi objective colloidal gold kit for acute injury of kidney Quantitative detection |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113125750A (en) * | 2021-04-03 | 2021-07-16 | 江西格朗生物技术有限公司 | Kidney injury molecule 1(Kim-1) detection reagent card |
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2017
- 2017-06-14 CN CN201720687739.3U patent/CN206772984U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113125750A (en) * | 2021-04-03 | 2021-07-16 | 江西格朗生物技术有限公司 | Kidney injury molecule 1(Kim-1) detection reagent card |
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