CN113125750A - Kidney injury molecule 1(Kim-1) detection reagent card - Google Patents
Kidney injury molecule 1(Kim-1) detection reagent card Download PDFInfo
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- CN113125750A CN113125750A CN202110364028.3A CN202110364028A CN113125750A CN 113125750 A CN113125750 A CN 113125750A CN 202110364028 A CN202110364028 A CN 202110364028A CN 113125750 A CN113125750 A CN 113125750A
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- kim
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- kidney injury
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6893—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids related to diseases not provided for elsewhere
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/577—Immunoassay; Biospecific binding assay; Materials therefor involving monoclonal antibodies binding reaction mechanisms characterised by the use of monoclonal antibodies; monoclonal antibodies per se are classified with their corresponding antigens
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
- G01N2333/705—Assays involving receptors, cell surface antigens or cell surface determinants
- G01N2333/70503—Immunoglobulin superfamily, e.g. VCAMs, PECAM, LFA-3
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2800/00—Detection or diagnosis of diseases
- G01N2800/34—Genitourinary disorders
- G01N2800/347—Renal failures; Glomerular diseases; Tubulointerstitial diseases, e.g. nephritic syndrome, glomerulonephritis; Renovascular diseases, e.g. renal artery occlusion, nephropathy
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Abstract
The invention provides a renal injury molecule 1(Kim-1) detection reagent card, and relates to the technical field of medical detection. The reagent card for detecting the kidney injury molecules (Kim-) comprises a clamping plate, wherein the top surface of the clamping plate is fixedly connected with the bottom end of contrast paper, a groove body is arranged on the right side of the top surface of the clamping plate, a reagent layer is arranged on the top surface of the clamping plate, the left end of the reagent layer is fixedly connected with the right end of the contrast paper, the components of the reagent layer are a Kim-monoclonal antibody, an anti-mouse IgG polyclonal antibody, a solid-phase nitrocellulose membrane and an anti-Kim-monoclonal antibody marked by colloidal gold respectively, a waterproof layer is arranged on the outer wall of the clamping plate, and a protective wax layer is arranged on the outer wall of the contrast paper. Be provided with the waterproof layer through the outer wall at the cardboard, be provided with the protection wax layer at the outer wall of contrast paper, utilize waterproof layer and protection wax layer to mutually support, increase overall structure's waterproof nature, guarantee its accuracy that detects data simultaneously.
Description
Technical Field
The invention relates to the technical field of medical detection, in particular to a kidney injury molecule 1(Kim-1) detection reagent card.
Background
Kidney injury molecule 1(Kim-1) is a transmembrane protein (type I membrane glycoprotein), and the RNA blot hybridization analysis result shows that the kidney injury molecule 1 is not expressed in fetal liver and fetal kidney, weakly expressed in normal adult liver, kidney and spleen, and obviously enhanced in the injured kidney tissue, and the research shows that the product after the rupture of the outer functional region of Kim-1 can be discharged through urine, so that the detection of the Kim-1 level in urine can indirectly evaluate the kidney injury condition.
There are also many detection reagent cards for kidney injury molecule 1(Kim-1) on the market, but the data cannot be visually displayed during detection, so that the accuracy of the detection data is low, meanwhile, the waterproof performance of the reagent card is not high, and the reagent layer is not protected, so that the reagent card is easily polluted and the inaccuracy of the detection data is caused.
Disclosure of Invention
Technical problem to be solved
Aiming at the defects of the prior art, the invention provides a reagent card for detecting kidney injury molecule 1(Kim-1), which solves the problems that the accuracy of detected data is low because the data cannot be visually displayed during detection, the waterproof performance of the reagent card is not high, and a reagent layer is not protected and is easy to be polluted, so that the detected data is inaccurate.
(II) technical scheme
In order to achieve the purpose, the invention is realized by the following technical scheme: the utility model provides a kidney injury molecule (Kim-) detect reagent card, includes the cardboard, the top surface of cardboard and the bottom fixed connection of contrast paper are in the same place, the top surface right side of cardboard is provided with the cell body, the top surface of cardboard is provided with the reagent layer, the left end on reagent layer and the right-hand member fixed connection of contrast paper are in the same place.
Preferably, the reagent layer comprises a Kim-monoclonal antibody, an anti-mouse IgG polyclonal antibody, a solid-phase nitrocellulose membrane and a colloidal gold-labeled anti-Kim-monoclonal antibody.
Preferably, the outer wall of the clamping plate is provided with a waterproof layer.
Preferably, the outer wall of the contrast paper is provided with a protective wax layer.
Preferably, the outer wall of the reagent layer is provided with a protective cover.
Preferably, the bottom end of the reagent layer is fixedly connected with the inner wall of the groove body in the clamping plate.
The working principle is as follows: firstly, collecting urine, collecting a urine specimen in a clean and dry container, and collecting 1-5ml of urine in the early morning or 24 hours; then, using a test tube to suck a urine specimen, dripping 2-3 drops of the urine specimen into a groove body of a test paper card, observing the result within 5-10 minutes, and reading the result within 30 minutes; and finally, observing the phenomenon on the surface of the test paper, wherein when two red bands appear, the kidney injury molecule 1(Kim-1) is detected in the test sample, when only one red band appears, the kidney injury molecule 1(Kim-1) is not detected in the test sample, and when the red band does not appear, the test failure or the reagent failure is indicated.
(III) advantageous effects
The invention provides a kidney injury molecule 1(Kim-1) detection reagent card. The method has the following beneficial effects:
1. according to the kidney injury molecule 1(Kim-1) detection reagent card, the components of a reagent layer are a Kim-monoclonal antibody and an anti-mouse IgG polyclonal antibody, and the Kim-monoclonal antibody is respectively a solid-phase nitrocellulose membrane and a colloidal gold-labeled anti-Kim-monoclonal antibody, so that an immunochromatography double-antibody sandwich method is realized, and the accuracy of detection data is ensured.
2. This kidney injury molecule 1(Kim-1) detect reagent card is through being provided with the waterproof layer at the outer wall of cardboard, is provided with the protection wax layer at the outer wall of contrast paper, utilizes waterproof layer and protection wax layer to mutually support, increases overall structure's waterproof nature, guarantees its accuracy that detects data simultaneously.
3. According to the kidney injury molecule 1(Kim-1) detection reagent card, the protective cover is arranged on the outer wall of the reagent layer, the reagent layer can be effectively prevented from being polluted by the protective cover, and the accuracy in detection is guaranteed.
Drawings
FIG. 1 is a schematic structural diagram of the present invention.
Wherein, 1, a clamping plate; 2. a trough body; 3. a reagent layer; 4. comparing paper; 5. a waterproof layer; 6. a protective wax layer; 7. a shield.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The first embodiment is as follows:
as shown in FIG. 1, the embodiment of the invention provides a reagent card for detecting a kidney injury molecule 1Kim-1, which comprises a clamping plate 1, wherein the top surface of the plate 1 is fixedly connected with the bottom end of a contrast paper 4, a groove body 2 is arranged on the right side of the top surface of the clamping plate 1, a reagent layer 3 is arranged on the top surface of the clamping plate 1, and the left end of the reagent layer 3 is fixedly connected with the right end of the contrast paper 4.
Example two:
the test tube is used for absorbing the urine specimen, 2-3 drops are added into the groove body of the clamping plate 1, the result is observed for 5-10 minutes, the phenomenon of the clamping plate 1 is observed based on reading within 30 minutes, when two red strips appear, the kidney injury molecule 1(Kim-1) is detected in the test specimen, when only one red strip appears, the kidney injury molecule 1(Kim-1) is not detected in the test specimen, and when no red strip appears, the test failure or the reagent failure is indicated.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (6)
1. A kidney injury molecule 1(Kim-1) detection reagent card comprises a clamping plate (1) and is characterized in that: the top surface of cardboard (1) and the bottom fixed connection of contrast paper (4) are in the same place, the top surface right side of cardboard (1) is provided with cell body (2), the top surface of cardboard (1) is provided with reagent layer (3), the left end of reagent layer (3) and the right-hand member fixed connection of contrast paper (4) are in the same place.
2. The kidney injury molecule 1(Kim-1) detection reagent card of claim 1, wherein: the reagent layer (3) comprises the components of a Kim-1 monoclonal antibody, an anti-mouse IgG polyclonal antibody, a solid-phase nitrocellulose membrane and a colloidal gold-labeled anti-Kim-1 monoclonal antibody.
3. The kidney injury molecule 1(Kim-1) detection reagent card of claim 1, wherein: the outer wall of the clamping plate (1) is provided with a waterproof layer (5).
4. The kidney injury molecule 1(Kim-1) detection reagent card of claim 1, wherein: and a protective wax layer (6) is arranged on the outer wall of the contrast paper (4).
5. The kidney injury molecule 1(Kim-1) detection reagent card of claim 1, wherein: and a protective cover (7) is arranged on the outer wall of the reagent layer (3).
6. The kidney injury molecule 1(Kim-1) detection reagent card of claim 1, wherein: the bottom end of the reagent layer (3) is fixedly connected with the inner wall of the groove body (2) in the clamping plate (1).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110364028.3A CN113125750A (en) | 2021-04-03 | 2021-04-03 | Kidney injury molecule 1(Kim-1) detection reagent card |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110364028.3A CN113125750A (en) | 2021-04-03 | 2021-04-03 | Kidney injury molecule 1(Kim-1) detection reagent card |
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| CN113125750A true CN113125750A (en) | 2021-07-16 |
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| CN202110364028.3A Pending CN113125750A (en) | 2021-04-03 | 2021-04-03 | Kidney injury molecule 1(Kim-1) detection reagent card |
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Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101419235A (en) * | 2008-09-24 | 2009-04-29 | 吉林大学 | KIM-1 detection kit and method for making same |
| CN102353782A (en) * | 2010-12-31 | 2012-02-15 | 上海华谷生物技术有限公司 | In vitro diagnostic kit of antihuman kidney injury molecule-1 and its detection method |
| US20130165338A1 (en) * | 2010-06-17 | 2013-06-27 | Max-Delbrueck-Centrum Fuer Molekulare Medizin | Biomarkers for determination of temporal phase of acute kidney injury |
| CN104226388A (en) * | 2014-09-20 | 2014-12-24 | 桂林理工大学 | Laser-assisted disposable test paper making method |
| US20170219608A1 (en) * | 2014-05-22 | 2017-08-03 | Wenbo HU | Kit for rapidly testing myocardial infarction and a preparation method and an application thereof |
| CN206772984U (en) * | 2017-06-14 | 2017-12-19 | 江苏扬新生物医药有限公司 | A kind of multi objective colloidal gold kit for acute injury of kidney Quantitative detection |
| CN208026750U (en) * | 2018-02-12 | 2018-10-30 | 新疆卡纳思生物技术有限公司 | Procalcitonin PCT quantitative determination reagent kits |
| CN208596168U (en) * | 2018-08-23 | 2019-03-12 | 广州市康润生物制品开发有限公司 | Toxoplasmosis IgM antigen testing reagent box |
| CN210720407U (en) * | 2019-07-02 | 2020-06-09 | 内蒙古医科大学附属人民医院(内蒙古自治区肿瘤医院) | Novel biomarker detection reagent box |
| CN211741310U (en) * | 2020-03-11 | 2020-10-23 | 中南大学湘雅医院 | Rapid detection card for chronic kidney disease marker suPAR |
-
2021
- 2021-04-03 CN CN202110364028.3A patent/CN113125750A/en active Pending
Patent Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101419235A (en) * | 2008-09-24 | 2009-04-29 | 吉林大学 | KIM-1 detection kit and method for making same |
| US20130165338A1 (en) * | 2010-06-17 | 2013-06-27 | Max-Delbrueck-Centrum Fuer Molekulare Medizin | Biomarkers for determination of temporal phase of acute kidney injury |
| CN102353782A (en) * | 2010-12-31 | 2012-02-15 | 上海华谷生物技术有限公司 | In vitro diagnostic kit of antihuman kidney injury molecule-1 and its detection method |
| US20170219608A1 (en) * | 2014-05-22 | 2017-08-03 | Wenbo HU | Kit for rapidly testing myocardial infarction and a preparation method and an application thereof |
| CN104226388A (en) * | 2014-09-20 | 2014-12-24 | 桂林理工大学 | Laser-assisted disposable test paper making method |
| CN206772984U (en) * | 2017-06-14 | 2017-12-19 | 江苏扬新生物医药有限公司 | A kind of multi objective colloidal gold kit for acute injury of kidney Quantitative detection |
| CN208026750U (en) * | 2018-02-12 | 2018-10-30 | 新疆卡纳思生物技术有限公司 | Procalcitonin PCT quantitative determination reagent kits |
| CN208596168U (en) * | 2018-08-23 | 2019-03-12 | 广州市康润生物制品开发有限公司 | Toxoplasmosis IgM antigen testing reagent box |
| CN210720407U (en) * | 2019-07-02 | 2020-06-09 | 内蒙古医科大学附属人民医院(内蒙古自治区肿瘤医院) | Novel biomarker detection reagent box |
| CN211741310U (en) * | 2020-03-11 | 2020-10-23 | 中南大学湘雅医院 | Rapid detection card for chronic kidney disease marker suPAR |
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