CN2864679Y - Detecting paper for pylorus spiral bacillus - Google Patents
Detecting paper for pylorus spiral bacillus Download PDFInfo
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- CN2864679Y CN2864679Y CN 200520113520 CN200520113520U CN2864679Y CN 2864679 Y CN2864679 Y CN 2864679Y CN 200520113520 CN200520113520 CN 200520113520 CN 200520113520 U CN200520113520 U CN 200520113520U CN 2864679 Y CN2864679 Y CN 2864679Y
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- helicobacter pylori
- monoclonal antibody
- test paper
- antibody
- nitrocellulose filter
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Abstract
Disclosed is a detection and test paper of helicobacter pylori, for detecting dejecta helicobacter pylori antigen based on the method of colloidal gold, pertaining to the colloidal gold immunoassay field. This test paper is made from a soleplate of test paper, cellulose nitrate membrane and colloidal gold-labeled pad. The middle of the soleplate is nitrocellulose membrane, which is provided with two lines, one of which is the reaction line of helicobacter pylori monoclonal antibody and the other of which is the control line of goat-anti-mouse polyclonal antibody, the end head of one end of the soleplate is the water absorption layer and that of the other end is glass fiber sample layer, and both ends of cellulose nitrate membrane are mutually overlapped and connected with the water absorption board and the gold-labeled pad of monoclonal antibody, on which is pressed with the glass fiber sample layer. Helicobacter pylori monoclonal antibody is used as a solid-phase capture antibody and detection antibody, the used antibody is to use recombinant antigen to immunize small mice, and two pairs of specific monoclonal antibody are selected. This method is provided with strong specificity, high sensitivity and low cost, and is easily operated, thereby facilitating to store and transport.
Description
Technical field
The utility model is that a kind of collaurum method of utilizing detects helicobacter pylori, belongs to the colloid gold immune detection range.
Background technology
Helicobacter pylori (Helicobacter pylori Hp) is one of human modal pathogenic bacteria, and China general population's infection rate is 50%-80%, and still with the speed increment of annual 1%-2%.Hp infects not only the substantial connection that has with Type B gastritis and peptic ulcer, and with non-ulcer dyspepsia, MALT lymthoma and cancer of the stomach important relationship is arranged also, so listed in first kind procarcinogen by international cancer research institution of the World Health Organization (WHO).
Detection methods such as generally acknowledged at present microbe growth, breath test (UBT), tissue staining, though have high sensitivity and specificity, can be used as Hp Infect And Diagnose standard, but big, the required instrument costliness of said method misery, operation steps loaded down with trivial details time-consuming, need the professional.Helicobacter pylori is grown under little aerobic environment, and condition of culture requires high, is difficult for survival, microbe growth is done clinical diagnosis cause very big difficulty, is difficult for promoting.And breath test is still unresolved to the pollution of patient and medical worker and surrounding environment.
Utilize immunological method two kinds of approach to be arranged for the detection of helicobacter pylori, the one, detect antibody, the 2nd, detect antigen.Detecting antibody needs serum as test sample, and detecting the antigen noninvasive method then can be by detecting ight soil antigen as the means that detect.Now existing a lot of documents are reported helicobacter pylori ight soil antigen, detect ight soil antigen and have clinical detection meaning and value equally, can be used as clinical diagnosis foundation and result of treatment and observe.The ELISA method detects ight soil antigen to be had than high specific and susceptibility by clinical approval, but needs professional and instrument, and the running time is long, and cost height, kit need refrigeration, and transportation is inconvenient.
Utilize the immune colloid gold method to realize to the ight soil detection of antigens, the method high specificity, highly sensitive, cost is low, easy and simple to handle, be convenient to store and transportation the domestic existing patent of the method, patent is 01223042 pylori antigen fast detecting box, and in this patent specification the 3rd page of the 10th row described be as solid-phase capture antibody with detect antibody with polyclone Hp antibody.The present invention makes with double antibodies sandwich method principle, is to utilize the Hp monoclonal antibody as solid-phase capture antibody and detection antibody.Characteristics such as monoclonal antibody has high specificity, and is highly sensitive, and difference between batch is little.
Summary of the invention
The purpose of this utility model provides a kind of helicobacter pylori detection test paper, has quick, sensitive, accurate, advantages of simple operation, has simultaneously and preserves conveniently, the characteristics of term of validity length.
In order to address the above problem, the present invention is achieved by the following technical solutions.
A kind of stripe shape is used to detect the test paper of helicobacter pylori, and it is to be made by test paper base plate, nitrocellulose filter, collaurum mark pad.Wherein test paper base plate (7) middle part is nitrocellulose filter (2), two lines are arranged on the nitrocellulose filter (2),, a helicobacter pylori monoclonal antibody reactive line (4) and a sheep anti mouse polyclonal antibody control line (3), in base plate (7) one end terminations is water accepting layer (1), other end termination is glass fiber sample layer (6), nitrocellulose filter (2) two ends overlap mutually with helicobacter pylori monoclonal antibody gold mark pad (5) with water accepting layer (1) respectively and are connected, and are pressed with glass fiber sample layer (6) on helicobacter pylori monoclonal antibody gold mark pad (5).This detects test paper and can be made into bar formula and board-like two kinds, assembling about colloidal gold test has been known technology, Wanhuapuman Bioengineering Co Ltd obtained the traditional Chinese medicines word authentication code of fecal occult blood colloidal gold test in 1998: the accurate word S19980076 of traditional Chinese medicines number, and with launch products.
It is the stripe shape test paper to be put into plate make that a kind of template is used for detecting the making of helicobacter pylori detection box, and it is formed by detecting test paper by upper plate, lower plate and stripe shape, and upper plate has display window and application of sample mouth as a result.
Pylori antigen with reorganization is carried out immunity, and finishing screen is selected two strains and had specificity pairing monoclonal antibody at the different determinants of Hp antigen, is designated as Hp-1 and Hp-2.One of them Hp-1 monoclonal antibody is fixed in response line place on the nitrocellulose filter, another Hp-2 monoclonal antibody is carried out colloid gold label, make Hp gold mark pad.
Be fixed with two lines on the nitrocellulose filter, a response line is that the Hp-1 monoclonal antibody specific is made, and another is that the sheep anti mouse polyclonal antibody is made.Hp-1 specific monoclonal antibody and sheep mouse-anti polyclonal antibody are individually fixed on the nitrocellulose filter, and two lines and golden mark level up the row placement and the Hp monoclonal antibody specific is marked between the pad at sheep anti mouse polyclonal antibody and gold.
Press known technology with base plate, nitrocellulose filter and monoclonal antibody gold mark pad, thieving paper is assembled.During detection, test paper is put into the inspection sample measuring liquid, liquid level must not surpass MAX line (8), carries out the result to specifications and judges.
Helicobacter pylori detection test paper according to such scheme is realized has quick, sensitive, accurate, advantages of simple operation, has simultaneously and preserves conveniently, the characteristics of term of validity length.
Description of drawings
Fig. 1 detects the main TV structure figure of test paper for helicobacter pylori.
Fig. 2 is the sectional structure chart of overlooking of Fig. 1 helicobacter pylori.
Fig. 3 is shown as negative findings figure when test paper is used to test for helicobacter pylori detects.
Fig. 4 is shown as positive findings figure when test paper is used to test for helicobacter pylori detects.
1. water accepting layer 2. nitrocellulose filters 3 control lines 4. response lines 5. gold medals mark fills up 6. glass fiber sample layers, 7. base plate 8.MAX line.
Concrete performance
Fig. 1～4 have been represented outside drawing of the present utility model in detail, and it is to be made by test paper base plate, nitrocellulose filter, collaurum mark pad.Wherein test paper base plate (7) middle part is nitrocellulose filter (2), two lines are arranged on the nitrocellulose filter (2),, a helicobacter pylori monoclonal antibody reactive line (4) and a sheep anti mouse polyclonal antibody control line (3), in base plate (7) one end terminations is water accepting layer (1), other end termination is glass fiber sample layer (6), nitrocellulose filter (2) two ends overlap mutually with helicobacter pylori monoclonal antibody gold mark pad (5) with water accepting layer (1) respectively and are connected, and are pressed with glass fiber sample layer (6) on helicobacter pylori monoclonal antibody gold mark pad (5).
1. helicobacter pylori MONOCLONAL ANTIBODIES SPECIFIC FOR
Pylori antigen with reorganization is carried out immunity to mouse, sets up the knurl strain with the SP20 Fusion of Cells and screens, and gets oncocyte and is injected in the BALB/c mouse abdominal cavity, makes it produce ascites.Extract the screening of mouse ascites purifying, finishing screen is selected two strains and is had strong specificity pairing Hp monoclonal antibody, is designated as Hp-1 and Hp-2.
The preparation of collaurum and with the combining of pylori spiral bacilli antibody
(1) get distilled water and add an amount of gold chloride magnetic agitation and be warmed to 90～95 ℃, add an amount of citric acid three and receive and continue heated and stirred to seething with excitement 5 minutes, it is standby to keep in Dark Place after the cooling.
(2) the collaurum liquid of pylori spiral bacilli antibody mark, on the adsorbing fiber material, dry back is standby.
3. film-making machine system film: utilize computer control transmission speed, guarantee that the antibody amount of bag quilt on the per unit film equates.
4. test strips combination: press the known technology combination.
5. test strips is put into plate and made helicobacter pylori template detection box, it is by being made up of upper plate, lower plate and detection test paper, and upper plate has display window and application of sample mouth as a result.
6. using method: gather the ight soil test sample, dilute with dilution, the sample imbibition end of stripe shape test paper is put into dilution, liquid level must not surpass the MAX line.Board-like detection box is that 01258826.1 the closet of adopting is sampled with patent, dilution is dripped to the application of sample mouth place of check-out console.Because syphonic effect, liquid moves judged result in 5 minutes through gold mark pad, response line and control line to the handle end of test paper.
7. the result judges: when in the ight soil pylori antigen being arranged, the colour developing district of test paper has two lines to show, and promptly response line and control line all become redness, and be positive.When in the ight soil during no pylori antigen, the colour developing district of test paper only has a control line to become redness, and is negative.When having color, the colour developing district of test paper do not show that represent that then misoperation or test paper are expired, this result is invalid.
Claims (6)
1. a helicobacter pylori detects test paper, it is characterized in that test paper base plate (7) middle part is nitrocellulose filter (2), two lines are arranged on the nitrocellulose filter (2),, a helicobacter pylori monoclonal antibody reactive line (4) and a sheep anti mouse polyclonal antibody control line (3), in base plate (7) one end terminations is water accepting layer (1), other end termination is glass fiber sample layer (6), nitrocellulose filter (2) two ends overlap mutually with helicobacter pylori monoclonal antibody gold mark pad (5) with water accepting layer (1) respectively and are connected, and are pressed with glass fiber sample layer (6) on helicobacter pylori monoclonal antibody gold mark pad (5).
2. a test paper that is used to detect helicobacter pylori is characterized in that used antibody is to utilize recombinant antigen that mouse is carried out immunity, filters out the specific helicobacter pylori pairing of two strains monoclonal antibody, is designated as Hp-1 and Hp-2.
3. according to claim 1 or 2 described a kind of test paper that are used to detect helicobacter pylori, it is characterized in that the Hp-1 monoclonal antibody is fixed in the response line place of nitrocellulose filter.
4. according to claim 1 or 2 described a kind of test paper that are used to detect helicobacter pylori, it is characterized in that the Hp-2 monoclonal antibody making Hp gold mark pad as golden labelled antibody.
5. according to the described a kind of test paper that is used to detect helicobacter pylori of claim 1, it is characterized in that having on the nitrocellulose filter two lines, article one, response line is that the helicobacter pylori monoclonal antibody specific is made, and another control line is that the sheep anti mouse polyclonal antibody is made.
6. according to the described a kind of test paper that is used to detect helicobacter pylori of claim 1, it is characterized in that helicobacter pylori specific monoclonal antibody and sheep anti mouse polyclonal antibody are individually fixed on the nitrocellulose filter, two lines and golden mark level up the row placement and the helicobacter pylori monoclonal antibody specific is marked between the pad at sheep anti mouse polyclonal antibody and gold.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200520113520 CN2864679Y (en) | 2005-07-15 | 2005-07-15 | Detecting paper for pylorus spiral bacillus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200520113520 CN2864679Y (en) | 2005-07-15 | 2005-07-15 | Detecting paper for pylorus spiral bacillus |
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| Publication Number | Publication Date |
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| CN2864679Y true CN2864679Y (en) | 2007-01-31 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN 200520113520 Expired - Fee Related CN2864679Y (en) | 2005-07-15 | 2005-07-15 | Detecting paper for pylorus spiral bacillus |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102288757A (en) * | 2011-07-12 | 2011-12-21 | 珠海市银科医学工程有限公司 | Non-invasive one-step method stomach helicobacter pylori detection kit and detection method |
| CN102393460A (en) * | 2011-08-22 | 2012-03-28 | 万积成 | Rapid detection device for helicobacter pylori |
| CN102636641A (en) * | 2012-03-26 | 2012-08-15 | 上海凯创生物技术有限公司 | Detection kit of helicobacter pylori emulsion method and preparation process thereof |
| CN103278637A (en) * | 2013-06-05 | 2013-09-04 | 姜竹泉 | Carbon nanotube test paper for detecting helicobacter pylori, and preparation method thereof |
| CN104897892A (en) * | 2015-06-01 | 2015-09-09 | 上海凯创生物技术有限公司 | Helicobacter pylori antigen colloidal gold detection kit |
-
2005
- 2005-07-15 CN CN 200520113520 patent/CN2864679Y/en not_active Expired - Fee Related
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102288757A (en) * | 2011-07-12 | 2011-12-21 | 珠海市银科医学工程有限公司 | Non-invasive one-step method stomach helicobacter pylori detection kit and detection method |
| CN102393460A (en) * | 2011-08-22 | 2012-03-28 | 万积成 | Rapid detection device for helicobacter pylori |
| CN102636641A (en) * | 2012-03-26 | 2012-08-15 | 上海凯创生物技术有限公司 | Detection kit of helicobacter pylori emulsion method and preparation process thereof |
| CN102636641B (en) * | 2012-03-26 | 2014-06-25 | 上海凯创生物技术有限公司 | Detection kit of helicobacter pylori emulsion method and preparation process thereof |
| CN103278637A (en) * | 2013-06-05 | 2013-09-04 | 姜竹泉 | Carbon nanotube test paper for detecting helicobacter pylori, and preparation method thereof |
| CN103278637B (en) * | 2013-06-05 | 2015-09-30 | 姜竹泉 | A kind of carbon nano-tube Test paper detecting helicobacter pylori and preparation method thereof |
| CN104897892A (en) * | 2015-06-01 | 2015-09-09 | 上海凯创生物技术有限公司 | Helicobacter pylori antigen colloidal gold detection kit |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20070131 Termination date: 20100715 |