TW200938626A - An algae hydrolysis and its manufacture process - Google Patents

An algae hydrolysis and its manufacture process Download PDF

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Publication number
TW200938626A
TW200938626A TW97109055A TW97109055A TW200938626A TW 200938626 A TW200938626 A TW 200938626A TW 97109055 A TW97109055 A TW 97109055A TW 97109055 A TW97109055 A TW 97109055A TW 200938626 A TW200938626 A TW 200938626A
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Taiwan
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milk
algae
enzyme
microalgae
decomposition
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TW97109055A
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Chinese (zh)
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Wen-Chen Chiu
Ying-Chu Chen
Ching-Lung Chen
Ping-Jui Yu
Pang-Wen Yang
Chih-Yung Liao
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Tensall Bio Tech Co Ltd
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Priority to TW97109055A priority Critical patent/TW200938626A/en
Publication of TW200938626A publication Critical patent/TW200938626A/en

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Abstract

This invention is aimed to add the economic value of algae (i.e. spiral, green, and red algae). It increases digestion & absorption of human gastr ointestinal tract, improves the stinky fish flavor, and lowers the cost. There are two steps in the invention including: (1) destroying the cell wall of algae through enzyme reaction to release the cytosolic nutrients; (2) decomposing the larger cytosolic nutrients into smaller molecules through probiotics to promote human digestion and absorption. No energy is needed and all process is under 25-37 DEG C.

Description

200938626 九、發明說明: 【發明所屬之技術領域】 螺旋藻(藍綠藻)的原主要產地在非洲和墨西哥,生長在驗性 的鹽湖中。三十五億年朗旋藻已存活在地球上,可以說是萬物 得以生存演化之母^據達爾文的生物演化論,史上越早出現而 且進化越遲緩的生物’具有越完整的營養源。經國内外大量科學 研究試驗證明,職藻在降低膽固醇和血脂、抗癌、減肥、養胃 護胃、治療貧血及微量元素缺乏、護肝、增進免疫、調整代賴 能等方面都有積極仙,被聯合國食品齡減為“二十一世紀 最理想的營養源,,。職藻含有天然食物中最豐富的蛋白質,含 1尚達70 %,相當於牛肉的3倍、雞蛋的5倍、大豆的2倍、乾 酪的2.4倍,提供人體所需的必須氨基酸及非關鍵性氨基酸 (Non-essential Amino Acids & Essential amino Acids);必須氨基酸 ❹包括 Isoleucine,Leucine,Lysine,Methionine,Phenylalanine,200938626 IX. Description of the invention: [Technical field to which the invention belongs] The original major producing areas of spirulina (blue-green algae) are grown in the salt lakes of Africa and Mexico. The 3.5 billion-year-old spirulina has survived on the earth, and it can be said that it is the mother of survival and evolution of all things. According to Darwin's theory of biological evolution, the sooner the history emerges and the slower the evolution, the more complete the source of nutrients. A large number of scientific research experiments at home and abroad have proved that the occupational algae have positive positives in reducing cholesterol and blood lipids, anti-cancer, weight loss, stomach and stomach protection, treatment of anemia and trace element deficiency, liver protection, immune enhancement, and adjustment of the performance. By the United Nations food age reduced to "the most ideal source of nutrition in the 21st century, the occupational algae contains the most abundant protein in natural foods, including 1% up to 70%, equivalent to 3 times of beef and 5 times of eggs, Two times as much as soybeans and 2.4 times as much as cheese, providing essential amino acids and essential amino acids (Non-essential Amino Acids & Essential amino Acids); essential amino acids including Isoleucine, Leucine, Lysine, Methionine, Phenylalanine,

Threonine,Tryptophane和Valine。其特有的藻藍蛋白是天然的抗 癌物質,能夠提高淋巴細胞活性,增強人體免疫力。螺旋藻中維 他命及礦物質含量極為豐富,包括維生素B群、A、E、K與鋅、鐵、 鉀、鈣、鎂、磷、硒、碘等微量元素,可維持人體酸鹼值的平衡, 並減少疲勞感的產生。螺旋藻含有大量的7_次亞麻油酸 (GLA) ’是天然荷爾蒙的前驅物質,更是健腦益智、降血脂、血壓 及膽固醇的理想物質,大大的減輕動脈硬化及中風的機率。螺旋 5 200938626 藻中含有極豐富的葉綠素,是普通蔬菜含量的1〇倍以上,在人體 内可轉化為血紅素,對中和血液中毒素及改善過敏體質,消除呼 吸道及消化道炎症都有積極作用。螺旋藻中脂肪含量只有5%,且 不含膽固醇’可使人體在補充必要蛋白時避錢^過多熱量。 諸多研究報告證實職藻的保健功效,但是此具有高營養價 值的螺鶴,其細胞壁就是—種障礙,阻礙了細胞㈣容物的釋 ❹ 放⑽子營養成分無法完全分解為小分子單元,崎低人體腸 胃道的消化和魏。騎縣消化祕不佳_費者而言,其細 胞壁更形成了獲取高營養物質時牢不可破的障^。若直接以此未 破壁的螺旋藻提供給消費大眾,消化吸收率低,也相對的降低了 食用經濟效益。故發明—^ 紐找…π 預先献職義細胞壁來 古广 物’進—步有效的分解成直接可被人體吸收利用的 有^:ρ’例如:必需氨基酸、多元不飽和脂肪酸、單分 維生素、酵素等,提高 甸糖 品,實為-件重要的事。 有效提升經濟效益的健康食 【發明内容】 釋放=====㈣___及分解後 多其,_素合併生物性二:解 6 200938626 破壁及有效成分單元化之二階段製程。 月’j述第-階段酵素分解之製程,係指利用酵素於25_37。〇作用 微藻類植物,依選用微藻類植物細胞壁成分不同而添加適當酵 素’作用時間18-24小時。 前述第二階段生物性作用之製程,係指细活化之益生菌菌 株於25 37C作用於經酵素分解細胞壁後微藻類植物所釋放之内 *物,作用時間24-48小日夺’使其最終酸驗值介於4.0-4.2之間。 本發明的產物可進-步以噴霧賴機處理,依f要被製成各 種適D的劑型’在此可舉出的例子包括粉劑、膠囊、旋片、飲品, 但不僅限於此。 上述益生菌株’包括乳酸桿菌雜咖、鏈球菌屬 ("如㈣、足球菌屬内、明串球菌屬如讀 和雙成椁菌屬(Bifidobacterium)等。 上述益生關株可以單獨使用,也可以將不同種或不同株之 益生菌株做任何結合。 上述酵素’包括纖維分解酵素、多聽類分解酵素、澱粉分解 酵素、或綜合性酵素等。 上述微義植物,包括螺旋藻、綠藻、紅藻、裸蕩、金蒸、 輪藻、異鞭藻等。 上述最終微藻類植物分解液可進一步去除菌體步驟處理,該 去除菌體的步驟並無特別限制,可舉出的例子包括了離心及過遽。 200938626 【實施方式】 本發明第一階段先將微藻類植物以等比例和純水混合,於 25-37°C恆溫狀態和適當比例酵素混合均勻,作用18-24小時,形 成酵素微藻類植物混合液,此為微藻類植物細胞壁分解之第一階 段製程。將第一階段之酵素微藻類植物混合液,以適當1:6比例 與高濃度益生菌菌株於25-37°C發酵槽培養24-48小時,當酸鹼 值落在4.0左右時,即分解完成。利用此微藻類植物之分解產物, 〇 混合高分子量的賦形劑,以喷霧乾燥機處理,製成粉末固態劑型 或各種適合的劑型。Threonine, Tryptophane and Valencia. Its unique phycocyanin is a natural anti-cancer substance that enhances lymphocyte activity and enhances human immunity. Spirulina is rich in vitamins and minerals, including vitamin B group, A, E, K and zinc, iron, potassium, calcium, magnesium, phosphorus, selenium, iodine and other trace elements, which can maintain the balance of the body's pH. And reduce the occurrence of fatigue. Spirulina contains a large amount of 7-times linoleic acid (GLA), which is a precursor of natural hormones. It is also an ideal substance for brain and brain, lowering blood fat, blood pressure and cholesterol, greatly reducing the chance of arteriosclerosis and stroke. Spiral 5 200938626 Algae is rich in chlorophyll, which is more than 1〇 of the average vegetable content. It can be converted into heme in the human body. It can neutralize blood toxins and improve allergies, and eliminate inflammation of the respiratory tract and digestive tract. effect. Spirulina has a fat content of only 5% and does not contain cholesterol, which allows the body to avoid excessive heat when supplementing essential proteins. Many research reports have confirmed the health benefits of the algae, but this highly nutritious snail has a cell wall that is a barrier that hinders the release of cells (4). (10) The nutrients cannot be completely decomposed into small molecules. Low digestion and Wei in the human gastrointestinal tract. Riding the county to digest the secret is not good _ fee, the cell wall has formed an unbreakable barrier to obtain high nutrients ^. If this unbroken spirulina is directly supplied to the consumer, the rate of digestion and absorption is low, and the economic benefits of eating are relatively reduced. Therefore, the invention - ^ 纽 looking for ... π pre-dedication of the cell wall to the ancient wide object 'in step - effectively decomposed into directly absorbed by the body ^: ρ 'such as: essential amino acids, polyunsaturated fatty acids, single-vitamins , enzymes, etc., to improve the sugar product, it is - an important thing. Healthy foods that effectively improve economic benefits [Summary of the invention] Release ===== (4) ___ and after decomposition, _ 素 combined with biological two: solution 6 200938626 Two-stage process of breaking the wall and active ingredient unitization. The month-by-phase process of enzyme decomposition refers to the use of enzymes at 25_37. 〇 Role Microalgae plants, depending on the cell wall composition of the microalgae plant, add appropriate enzymes for 18-24 hours. The process of the second stage biological action refers to the action of the finely activated probiotic strain at 25 37C on the release of microalgae after the enzyme decomposes the cell wall, and the action time is 24-48 days. The acid value is between 4.0 and 4.2. The product of the present invention can be further processed by a spray, and it can be formulated into various D dosage forms. Examples which may be mentioned herein include powders, capsules, rotary tablets, and drinks, but are not limited thereto. The above probiotic strains include Lactobacillus sinensis, Streptococcus ("such as (4), genus of genus, genus Streptococcus such as genus and Bifidobacterium, etc. The above probiotics can be used alone, Any combination of different strains or different strains of probiotic strains can be used. The above enzymes include fibrinolytic enzymes, poly-degrading enzymes, amylolytic enzymes, or synthetic enzymes, etc. The above-mentioned micro-sense plants, including spirulina, green algae, The red algae, the naked algae, the golden steamed, the spirulina, the dinoflagellate, etc. The above-mentioned final microalgae plant decomposition liquid can further remove the bacterial body step, and the step of removing the bacteria is not particularly limited, and examples thereof include Centrifugation and sputum. 200938626 [Embodiment] In the first stage of the present invention, the microalgae plants are first mixed with pure water in an equal proportion and mixed at a constant temperature of 25-37 ° C and an appropriate ratio of enzymes for 18-24 hours to form. A mixture of enzyme microalgae plants, which is the first stage of the decomposition of the cell wall of microalgae plants. The first stage of the enzyme microalgae mixture is mixed at a proper ratio of 1:6. The high-concentration probiotic strain is cultured in a fermentation tank at 25-37 ° C for 24-48 hours, and when the pH value falls to about 4.0, the decomposition is completed. Using the decomposition product of the microalgae plant, the high molecular weight excipient is mixed with the mash. It is processed by a spray dryer to form a powder solid dosage form or various suitable dosage forms.

8 200938626 【圖式簡單說明】 將酵素與微藻類植物以適當比例混合,反應18-24小時;破 壁後加入益生菌菌株,25-37°C發酵槽培養24-48小時,當酸鹼 值洛在4.0左右時’即分解完成。8 200938626 [Simple description of the schema] The enzyme and microalgae plants are mixed in an appropriate ratio for 18-24 hours; after the wall is broken, the probiotic strain is added, and the fermentation tank is cultured at 25-37 ° C for 24-48 hours, when the pH is When Luo is around 4.0, the decomposition is completed.

Claims (1)

200938626 、申請專利範圍: 利用酵素合併生物性方 及有效成分單元化之二階段製 .本發明係指微藻類植物分解之製程, 法進行微藻類植物細胞破壁 程 2.如申凊專利範圍苐彳 落、έ卞 此_植物,係指螺旋藻、綠 “、,工藻、裸藻、金藻、輪藻、異鞭藻等。 ” '200938626, the scope of application for patents: the two-stage system using enzymes combined with biological side and active ingredient unitization. The present invention refers to a process of decomposition of microalgae plants, and the method of microalgae plant cell breaking process is as follows. Fallen, έ卞 this _ plant, refers to spirulina, green ",, algae, Euglena, algae, Chara, Isochrysis, etc." 3_ =申請專機圍第1猶叙第1鱗素麵之製程,酵 素係指纖維分解酵素、多_分解酵素、澱粉分 合性酵素等。 ''νΤ' 4·如申請專利範圍第,項所述之第二階段生物性作用之製程, 係才曰利用南濃度益生菌株培養液發酵分解微藻類植物之内 容物’形成小分子單元體。 5·如申請專利範圍第1項所述微藻類植物分解之製程,其最終 產物可進一步以去除菌體步驟處理,該去除菌體的步驟並無 特別限制’可舉出的例子包括了離心及過渡。 6·如申請專利範圍第1項所述健康食品之製程,產物可依需要 被製成各種適合的劑塑,在此可舉出的例子包括粉劑、膠 囊、錠片、飲品,但不僅限於此。 如申請專利範圍第4項所述之益生菌株包括乳酸桿菌屬 、鏈球菌屬 、足球菌屬 、明串球菌屬和雙歧桿菌屬 200938626 等。所使用菌株最好為嗜酸乳桿菌 (Lactobacillus acidophilus)、免絡乳择憲①actobacillus ciwez)、保加利亞菌改^acz7/w>s ’、嗜熱乳桿菌 (Streptococcus thermophilus)、乳珠蛰(Lactococcus lactis)。h 述益生菌菌株可以單獨使用,也可以將不同種或不同株之乳 酸菌做任何結合。 〇 鲁 如曱Μ專利範圍第4項所述高濃度益生g株培養液,係指利 用益^株朗培養於營養基_,營養基質包括奶類、豆 漿、茶等。上述域並無特麻制,可為多鶴物所產之乳 水,也可為還原乳。動物所產的乳水之可舉出的例子包括牛 ^和^奶’較佳者為牛奶。上述絲並無特舰制,可為黃 可為&欠“化丑、米丑等。上述茶類並無特別限制, 9如由、工木、綠命、烏龍茶、桂花茶、藥草茶等。 •申請專利範圍第4項所述高濃度 加充填劑、防妳抗氧化劑、pH=株培μ養液中,可添 自由基、或表面活性劑。 正劑、PH緩衝劑、除 10·如申請專利範 11-如申請專利範圍第9項所述之製程 葡t糖植物膠、阿拉伯踢等。 12_如申請專利範圚第9項所述之·, 糖、觀赠、山梨^之㈣,騎銳域糖、乳 防凍劑包括海藻糖、 抗氧化劑包括维生素 11 200938626 C、维生素E、氣基酸等。 13. 如申請專利範圍第9項所述之製程,pH調整劑包括碳酸氫 鈉、氫氧化鈉等。 14. 如申請專利範圍第9項所述之製程,pH緩衝劑包括磷酸缓 衝溶液等。 15. 如申請專利範圍第9項所述之製程,除自由基劑包括綠茶、 /3胡蘿蔔素、茄紅素(lycopene )等。 © 16·如申請專利範圍第9項所述之製程,表面活性劑包括卵磷 脂、蔗糖脂肪酸酯等。 ❹ 12 200938626 發獲專租說明書 .,>V,^*~·— Γ I |, | || . 97. 4. 1 |f jl| (本說月書格式、順序及粗逋字’請勿任意更動,※記號部分請勿填寫) ※申請案號^叫。0 u (/ ※申請日期※:EPC分類:冗 一、發明名稱:(中文/英文) C(2pA〇 U006.011 微藻類植物分解之製程 An algae hydrolysis and its manufacture process O 二、申請人:(共i人) 姓名或名稱:(中文/英文) 天賜爾生物科技股份有限公司/Tensall Bio-Tech Co.,LTD 代表人:(中文/英文)張平和/CHANQ PING HOE 住居所或營業所地址:(中文/英文) 宜蘭縣 269 冬山鄉梅花路 732 號/No.732, MeihuaRd.,Dongshan Township, Yilan County 269, Taiwan (R.O.C.) 國籍:(中文/英文)中華民國/R.O.C. ❹ 三、發明人:(共6人) 姓名:(中文/英文) 1.邱文貞/CHIU, WEN CHEN 2·陳盈竹/CHEN, YING CHU 3.P東慶龍/CHEN, CHING LUNG 4·游秉叡/YU, PING JUI 5.楊邦雯/YANG, PANG WEN 6·廖智勇/LIAO, CHIH YUNG 國籍:(中文/英文) 1〜6中華民國/R_O.C. 200938626 發明專利說明書 t3_ = Apply for the special machine around the 1st scale of the first scale, the enzyme refers to fiber decomposition enzymes, poly-degrading enzymes, starch-binding enzymes. ''νΤ' 4· The process of the second stage of biological action described in the scope of the patent application, the use of a medium concentration probiotic strain culture solution to ferment and decompose the contents of microalgae plants to form small molecule units. 5. The process of decomposing the microalgae plant according to claim 1 of the patent application, the final product may be further treated by the step of removing the bacteria, and the step of removing the bacteria is not particularly limited. The examples include centrifugation and transition. 6. If the process of the health food described in claim 1 is applied, the product may be prepared into various suitable plastics as needed, and examples thereof include powders, capsules, tablets, drinks, but not limited thereto. . Probiotic strains as described in claim 4 include Lactobacillus, Streptococcus, Staphylococcus, Streptococcus and Bifidobacterium 200938626. The strain to be used is preferably Lactobacillus acidophilus, 1actobacillus ciwez, Bulgarian strain ^acz7/w>s', Streptococcus thermophilus, Lactococcus lactis ). h The probiotic strains may be used alone or in combination with different or different strains of lactic acid bacteria. 〇 Lu Rugao The high-concentration probiotic g strain culture solution mentioned in item 4 of the patent scope refers to the use of beneficial nutrients to cultivate nutrients, and the nutritional matrix includes milk, soy milk, tea and the like. There is no special hemp system in the above-mentioned fields, and it can be milk produced by many cranes or reduced milk. Examples of the milk produced by the animal include bovine and milk, which are preferably milk. The above-mentioned silk has no special ship system, but it can be Huang Kewei & "Under ugly, rice ugly, etc. There are no special restrictions on the above teas, such as Yu, Gongmu, Luming, Oolong Tea, Osmanthus Tea, Herbal Tea, etc. • Apply high-concentration plus filler, anti-mite antioxidant, pH= strain culture solution in the fourth paragraph of the patent application, and add free radicals or surfactants. Positive agent, PH buffer, except 10· For example, the application of the patent model 11 - as described in the scope of claim 9 of the process of the Portuguese t-glycan gum, Arabian kick, etc. 12_ as claimed in the patent paradigm item 9, sugar, gift, Yamanashi ^ (4) riding an acute sugar, milk antifreeze including trehalose, antioxidants including vitamin 11 200938626 C, vitamin E, gas-based acid, etc. 13. As described in the scope of the patent application, the pH adjusting agent includes hydrogencarbonate Sodium, sodium hydroxide, etc. 14. The process of claim 9, wherein the pH buffer comprises a phosphate buffer solution, etc. 15. The process of claim 9, wherein the free radical agent comprises Green tea, /3 carotene, lycopene, etc. © 16·如In the process described in claim 9, the surfactants include lecithin, sucrose fatty acid esters, etc. ❹ 12 200938626 Issued the special rent specification., >V,^*~·— Γ I |, | || 97. 4. 1 |f jl| (This book format, order, and bold words are not allowed to be changed, please do not fill in the ※ part) ※Application number ^. 0 u (/ ※ application date ※ : EPC classification: redundant one, invention name: (Chinese / English) C (2pA 〇 U006.011 microalgae plant decomposition process An algae hydrolysis and its manufacture process O Second, the applicant: (total i people) Name or name: (Chinese / English) Tiansell Biotech Co., Ltd. / Tensall Bio-Tech Co., LTD Representative: (Chinese / English) Zhang Pinghe / CHANQ PING HOE Residence or business address: (Chinese / English) Yilan County 269 No.732, Meihua Road, Dongshan Township, No.732, MeihuaRd., Dongshan Township, Yilan County 269, Taiwan (ROC) Nationality: (Chinese/English) Republic of China/ROC ❹ III. Inventor: (Total 6 persons) Name: ( Chinese/English) 1. Qiu Wenzhao / CHIU, WEN CHEN 2·陈盈竹/CHEN, YING CHU 3.P Dongqinglong/CHEN, CHING LUNG 4·You Bingrui/YU, PING JUI 5.Yang Bangwen/YANG, PANG WEN 6·Liao Zhiyong/LIAO, CHIH YUNG Nationality: (Chinese /English) 1~6 Republic of China/R_O.C. 200938626 Patent Specification t ※申請曰期: αε π Χό u〇〇6.〇ii^PC分類:Q2p从削加·υ1』 {Z006.0U 一、發明名稱:(中文/英文) 微藻類植物分解之製程 An algae hydrolysis and its manufacture process ❹ 二、申請人:(共1人) 姓名或名稱:(中文/英文) 天賜爾生物科技股份有限公司/Tensall Bio-Tech Co.,LTD 代表人··(中文/英文)張平和/CHANCE PING HOE 住居所或營業所地址:(中文/英文) 宜蘭縣 269 冬山鄉梅花路 732 號/ No.732, MeihuaRd.,Dongshan Township, Yilan County 269, Taiwan (R.O.C.) 國籍:(中文/英文)中華民國/R.O.C. ❹三、發明人:(共6人) 姓名:(中文/英文) 1_邱文貞/CHIU, WEN CHEN 2_陳盈竹/CHEN, YING CHU 3.陳慶龍/CHEN, CHING LUNG 4_游秉叙/YU, PING JUI 5.揚邦雯/YANG, PANG WEN 6·廖智勇/LIAO, CHIH YUNG 國籍:(中文/英文) 1〜6中華民國/R.O_C. 200938626 四、聲明事項: □ mr日項□日第-款或□第二款狀之事實,其 □【:=向下列國家(‘二利: L格式凊依:受理國定γ □有主料偷第二十請日、㈣魏祕註記】 條第一項國際優先權·· 〇 D無主張專利法第二 十七條第一項國際優先權: D主張專利法第二十 【格式請依··申請曰申ff 一項國内優先權: 申请案號順序往記】 0 ^張專利法第三十條生物材料·· 一1須寄存生物材料者·· 國内生物材料【格式請依:寄存機構、日期、號碼順序註記】 國外生物椅料^ k式請依··寄存國家、機構、曰期、號碼順序註記】 3不須寄存生物材料者: 技街錢中具有通常知識者祕獲得時,不須寄存。 200938626 四、聲明事項: □主張專利法第二十二條第二項□第一款或□第二款規定之事實,其 事實發生日期為:年月曰。 二1申請前已向下列國家(地區)申請專利: 【格式請依:受理國家(地區)、申請曰、申請案號順序註記】 □有主張專利法第二十七條第一項國際優先權: □無主張專利法第二十七條第一項國際優先權·· □主張專利法第二十九條第一項國内優先權: 【格式請依:申請曰、申請案號順序註記】 ‘ 主張專利法第三十條生物材料: □須寄存生物材料者: 國内生物材料【格式請依:寄存機構、日期、號碼順序註記】 國外生物材料【格式請依··寄存國家、機構、日期、號碼順序註記】 ❹ _v_不須寄存生物材料者: 所屬技術領域中具有通常知識者易於獲得時,不須寄存。 2 200938626 九、發明說明: 【發明所屬之技術領域】 螺%藻(藍綠藻)的原主要產地在非洲和墨西哥,生長在驗性 的鹽湖中。三十五億年前螺旋藻已存活在地球上,可以說是萬物 得以生存演化之母。根據達爾文的生物演化論,史上越早出現而 且進化越遲緩的生物,具有越完整的營養源。經國内外大量科學 研究試驗證明,螺旋藻在降低膽固醇和血脂、抗癌、減肥、養胃 4月、治療貧血及微量元素缺乏、護肝、增進免疫、調整代謝機 旎等方面都有積極作用,被聯合國食品協會推薦為“二十一世紀 最理想的營養源,,。職藻含有天齡物中最豐富的蛋白質,含 量高達70 %,相當於牛肉的3倍、雞蛋的5倍、大豆的2倍、乾 路的2.4七,&供人體所需的必須氨基酸及非關鍵性氨基酸 (Non-essential Ammo Acids & Essential amino Acids);必須氨基酸 包括 Isoleudne, phenylaian_ Threonine,Tryptophane和VaHne。其特有的藻藍蛋白是天然的抗 癌物質提高淋巴細胞活性,增強人體免疫力。螺旋藻中維 他命及礦物質含量極為豐富,包括維生素β群、A、E、K與辞、鐵、 鉀鈣、鎂、鱗、砸、蛾等微量元素,可維持人舰驗值的平衡, 並減少疲勞感的產生。螺旋藻含有大量的I次亞麻油酸 (GLA),是天然荷爾蒙的前驅物質,更是健腦益智、降血脂、血壓 及膽固醇的理想物質’大大的減輕動脈硬化及中風的機率。職 200938626 九、發明說明: 【發明所屬之技術領域】 螺旋藻(藍綠藻)的原主要產地在非洲和墨西哥,生長在驗性 的鹽湖中。三十五億年前螺旋藻已存活在地球上,可以說是萬物 得以生存演化之母。根據達爾文的生物演化論,史上越早出現而 且進化越遲缓的生物’具有越完整的營養源。經國内外大量科學 研究試驗證明,螺旋藻在降低膽固醇和血脂、抗癌、減肥、養胃 © 護胃、治療貧血及微量元素缺乏、護肝、增進免疫、調整代謝機 能等方面都有積極作用,被聯合國食品協會推薦為“二十一世紀 最理想的營養源”。螺旋藻含有天然食物中最豐富的蛋白質,含 量高達70 %,相當於牛肉的3倍、雞蛋的5倍、大豆的2倍、乾 酪的2.4倍,提供人體所需的必須氨基酸及非關鍵性氨基酸 (Non-essential Amino Acids & Essential amino Acids);必須氨基酸 包括 Isoleucine,Leucine,Lysine,Methionine,Phenylalanine, © Tryptophane和Valine。其特有的藻藍蛋白是天然的抗 癌物質,能夠提高淋巴細胞活性,增強人體免疫力。螺旋藻中維 他命及礦物質含量極為豐富’包括維生素B群、A、E、K與辞、鐵、 鉀、鈣、鎂、磷、硒、碘等微量元素,可維持人體酸鹼值的平衡, 並減少疲勞感的產生。螺旋藻含有大量的次亞麻油酸 (GLA),是天然荷爾蒙的前驅物質,更是健腦益智、降血脂、血壓 及膽固醇的理想物質,大大的減輕動脈硬化及中風的機率。螺旋 5 200938626 藻中含有極豐富的葉綠素,是普通蔬菜含量的1〇倍以上,在人體 内可轉化為血紅素,對巾和血液巾毒素及改善過敏财,消除啤 吸道及消化道炎症都有積極作用。螺旋藻中脂肪含量只有5%,且 不含膽固醇,可使人體铜充必要蛋白時避賴人過多熱量。 Ο ❹ 諸多研究報告證實螺旋藻的保健功效,蚁此具有高營養價 值的螺U ’其細胞壁就是—瓣礙,阻礙了細胞伽容物的釋 =,高分子營養成分無法完全分解為小分子科,而降低人體腸 胃道的消化和吸收。對於原本消化系統不佳的消f者而言,其細 胞壁更形成了獲取高營養物f時牢不可破的障壁。若直接以此未 破壁的螺鶴提供給消費大眾,消化吸收率低,也相對的降低了 食用經濟效益。故發明—有效製程,預先消倾鶴的細胞壁來 釋放„出其内容物’進—步有效的分解成直接可被人體吸收利用的 有效單7L ’例如:必需氨基酸、多元不麵軸酸 酵素等,提高人赚率、有效提升經濟效益:康糖食 口口,貫為一件重要的事。 【發明内容】 並強化人體對=2=營養價值、高觀 '低成本的健康食品 之製程,t 傾收率。本發明係指微_植物分解 其目__酵素合併生祕方妓行微_植物細胞 200938626 ’藻中含有極豐富的葉綠素,是普通蔬菜含量的ι〇倍以上,在人體 内可轉化為血紅素’對中和血液中毒素及改善過敏雜,消除呼 都有積極伽。螺旋藻中麟含量只有5%,且 不s膽固醇,可使人體在補充必要蛋白時避免攝入過多熱量。 諸夕研究報實螺_的保健功效,但是此具有高營養價 值的,旋簾’其細胞壁就是一轉礙,阻礙了細胞壁内容物的釋 ❹Γ7 77子轉齡無妓全分解為小分子料,綺低人體腸 田、的4化和吸收。對於原本消化系統不佳的消費者而言,其細 胞壁更形成了獲取®營養物f時林可破轉壁。若直接以此未 ^壁的螺鶴提供給消費大眾,消化吸收率低,也相對的降低了 釋H效益。故發明—有效製程,預先消化螺旋細細胞壁來 有5、内谷物’進一步有效的分解成直接可被人體吸收利用的 效單7G ’例如:必需氨基酸、多元不飽和脂肪酸、料子糖、 維生紊、缺各哲 ❹ ^ 、、專’提高人體消化率、有效提升經濟效益的健康食 σσ,實為一件重要的事。 【發明内容】 為解^述之問題,有效分解微絲植物的細賴及分解後 的内谷物’提供高營養價值、高風味、低成本的健康食品 I強化人體對螺热# 之製程 %焚藻的消化吸收率。本發明係指微藻類植物分解 其目的係利用酵素合併生物性方法進行微藻類植物細胞 6 200938626 破壁及有效成分單元化之二階段製程。 月’J述第一階段酵素分解之製程,係指利用酵素於25_37。〇作用 微藻類植物,依選用微藻類植物細胞壁成分不同而添加適當酵 素’作用時間18-24小時。 刖述第二階段生物性作用之製程,係指利用活化之益生菌菌 株於25-37°C作用於經酵素分解細胞壁後微藻類植物所釋放之内※Application period: αε π Χό u〇〇6.〇ii^PC classification: Q2p from cutting plus υ1』 {Z006.0U I. Invention name: (Chinese / English) Process of microalgae plant decomposition An algae hydrolysis and Its manufacture process ❹ II. Applicant: (1 in total) Name: (Chinese/English) Tiancier Biotechnology Co., Ltd./Tensall Bio-Tech Co., LTD Representative (·Chinese/English) Zhang Pinghe /CHANCE PING HOE Address or place of business: (Chinese/English) 272 Meihua Road, Dongshan Township, Yilan County 269, No.732, MeihuaRd., Dongshan Township, Yilan County 269, Taiwan (ROC) Nationality: (Chinese/English ROC/ROC ❹3. Inventor: (6 in total) Name: (Chinese/English) 1_邱文贞/CHIU, WEN CHEN 2_陈盈竹/CHEN, YING CHU 3.Chen Qinglong/CHEN, CHING LUNG 4_You Bingxu /YU, PING JUI 5.Yang Bangwen/YANG, PANG WEN 6·Liao Zhiyong/LIAO, CHIH YUNG Nationality: (Chinese/English) 1~6 Republic of China/R.O_C. 200938626 IV. Declaration: □ mr Day Item No. - Day or □ Second paragraph Actually, its □[:= to the following countries ('two benefits: L format conversion: acceptance of national γ □ □ have the main material to steal the twentieth day, (four) Wei secret notes] the first international priority · 〇D No claim for patent law Article 27, first international priority: D advocates patent law twentieth [format please apply for 曰 ff a domestic priority: application case order] 0 ^ Zhang Article 30 of the Patent Law Biomaterials··1st person who needs to deposit biomaterials·· Domestic biomaterials [format please note: order of depository, date, number order] Foreign bio-chair material ^ k type please Notes on the order of countries, institutions, flood seasons and numbers] 3 Those who do not need to deposit biological materials: When there is a common knowledge in the street, there is no need to deposit them. 200938626 IV. Declarations: □ Proposal Article 22 of the Patent Law The second item □ the first paragraph or the second paragraph stipulates the fact that the date of occurrence is: year and month 曰. 21 The application has been applied to the following countries (regions) before the application: [Format please: Accepting country (region) , application, application number Note] □ There is a claim for the first international priority of Article 27 of the Patent Law: □ No claim for patent law Article 27, the first international priority·· □ Claim the first country of Article 29 of the Patent Law Internal priority: [Format please follow: application 曰, application case number note] 'Proclamation of patent law Article 30 Biological materials: □ Those who need to deposit biological materials: Domestic biological materials [format please follow: depository, date, Number sequence note] Foreign biomaterials [formats please follow the country, organization, date, number order note] ❹ _v_Do not need to deposit biomaterials: When you have the usual knowledge in the technical field, you don't need to register . 2 200938626 IX. Description of the invention: [Technical field to which the invention belongs] The original major producing area of snail algae (blue-green algae) is grown in an experimental salt lake in Africa and Mexico. Spirulina has survived on the earth 3.5 billion years ago, and it can be said that it is the mother of all things. According to Darwin's theory of biological evolution, the sooner the history emerges and the slower the evolution, the more complete the source of nutrients. A large number of scientific research experiments at home and abroad have proved that Spirulina has positive effects in reducing cholesterol and blood lipids, anti-cancer, weight loss, stomach maintenance in April, treatment of anemia and trace element deficiency, liver protection, immune enhancement, and adjustment of metabolic sputum. Recommended by the United Nations Food Association as "the most ideal source of nutrition in the 21st century, the occupational algae contains the most abundant protein in the age of up to 70%, which is equivalent to three times that of beef, five times that of eggs, and soybean. 2 times, 2.4 of the trunk, &Non-essential Ammo Acids & Essential amino Acids; essential amino acids include Isoleudne, phenylaian_ Threonine, Tryptophane and VaHne. The unique phycocyanin is a natural anti-cancer substance that enhances lymphocyte activity and enhances human immunity. Spirulina is rich in vitamins and minerals, including vitamin β group, A, E, K and rhetoric, iron, potassium and calcium. Trace elements such as magnesium, scales, mites, moths, etc., can maintain the balance of human ship value and reduce the occurrence of fatigue. Spirulina contains a large number of times. Sesame oleic acid (GLA), a precursor of natural hormones, is an ideal substance for brain and brain, lowering blood fat, blood pressure and cholesterol. It greatly reduces the chance of arteriosclerosis and stroke. Job 200938626 IX. Invention: The technical field of spirulina (blue-green algae) is mainly produced in Africa and Mexico, and is grown in an experimental salt lake. Spirulina has survived on the earth 3.5 billion years ago. It can be said that everything can survive and evolve. According to Darwin's theory of biological evolution, the sooner the history emerges and the slower the evolution of the organism's, the more complete the source of nutrients. Through a large number of scientific research experiments at home and abroad, spirulina is reducing cholesterol and blood lipids, fighting cancer, losing weight, Yangwei© has a positive effect on stomach protection, treatment of anemia and trace element deficiency, liver protection, immune enhancement, and adjustment of metabolic functions. It has been recommended by the United Nations Food Association as “the most ideal source of nutrition in the 21st century.” Spirulina contains The most abundant protein in natural foods, up to 70%, equivalent to 3 times that of beef, 5 times that of eggs, and 2 times that of soybeans. 2.4 times that of cheese, providing essential amino acids and essential amino acids for the human body; essential amino acids include Isoleucine, Leucine, Lysine, Methionine, Phenylalanine, © Tryptophane and Valencia. The phycocyanin is a natural anticancer substance that enhances lymphocyte activity and enhances human immunity. Vitamins and minerals are extremely abundant in spirulina 'including vitamin B group, A, E, K and rhetoric, iron, potassium, Trace elements such as calcium, magnesium, phosphorus, selenium and iodine can maintain the balance of the body's pH and reduce the feeling of fatigue. Spirulina contains a large amount of sub-linolenic acid (GLA), which is a precursor of natural hormones. It is also an ideal substance for brain and brain, lowering blood fat, blood pressure and cholesterol, greatly reducing the chance of arteriosclerosis and stroke. Spiral 5 200938626 Algae contains extremely rich chlorophyll, which is more than 1〇 of the average vegetable content. It can be converted into hemoglobin in the human body, toxins on towels and blood towels, improve allergies, eliminate beer suction and inflammation of the digestive tract. Have a positive effect. Spirulina has a fat content of only 5% and does not contain cholesterol, which can make the body's copper to avoid excessive heat when it is necessary protein. Ο 诸多 Many research reports have confirmed the health benefits of spirulina. The cytoplasmic wall of snails with high nutritional value is the barrier, which hinders the release of gamma-containing substances. The polymer nutrients cannot be completely decomposed into small molecules. , while reducing the digestion and absorption of the human gastrointestinal tract. For those who have a poor digestive system, the cell walls form an unbreakable barrier to high nutrient f. If this unbroken snail is directly supplied to the consumer, the rate of digestion and absorption is low, and the economic benefits of eating are relatively reduced. Therefore, the invention-effective process, pre-empting the cell wall of the crane to release the „out of its contents' is effectively decomposed into an effective single 7L that can be absorbed and utilized by the human body, for example: essential amino acids, polyunsaturated acid enzymes, etc. To improve the rate of earning people and effectively improve the economic benefits: Kang sugar food mouth, is an important thing. [Invention content] And strengthen the human body on the process of = 2 = nutritional value, high-view 'low-cost healthy food, t. The yield of the invention refers to the micro-plant decomposition of its target __ enzyme combined with the secret recipe 妓 妓 micro_plant cells 200938626 'The algae contains extremely rich chlorophyll, which is more than 〇 times the ordinary vegetable content, in the human body It can be converted into heme's to neutralize the toxins in the blood and improve the allergies, and eliminates the sputum. The spirulina has only 5% of the content of lin, and it does not contain cholesterol, which can prevent the body from over-replenishing when supplementing essential proteins. The heat of the Zhu Xi study reported the health effect of the snail _ _, but this has a high nutritional value, the curtain wall 'the cell wall is a hindrance, hindering the release of the contents of the cell wall 7 77 Age-innocent is completely decomposed into small molecular materials, which degrades the body's intestinal field, and the absorption and disintegration. For consumers who have poor digestive system, the cell wall is more formed when the nutrient f is obtained. If the snail crane is directly supplied to the consumer, the digestion and absorption rate is low, and the H benefit is relatively reduced. Therefore, the invention - effective process, pre-digestion of the spiral fine cell wall to have 5, the inner grain 'further effective It is decomposed into a single effect 7G that can be absorbed and utilized by the human body. For example: essential amino acids, polyunsaturated fatty acids, sugars, vitamins, lack of philosophies, and special 'improving the digestibility of the human body and effectively improving economic efficiency. Healthy food σσ is really an important thing. [Summary of the Invention] In order to solve the problem, it effectively decomposes the micro-filament plants and the decomposed inner grains to provide high nutritional value, high flavor and low cost of health. Food I strengthens the digestion and absorption rate of the body-burning algae. The invention refers to the decomposition of microalgae plants. The purpose is to use microbial planting with enzymes and biological methods. Cell 6 200938626 Broken wall and two-stage process of active ingredient unitization. The process of enzyme decomposition in the first stage of the month refers to the use of enzymes in 25_37. The action of microalgae plants is added according to the cell wall composition of microalgae plants. Appropriate enzyme 'action time 18-24 hours. The process of the second stage biological action refers to the use of activated probiotic strains at 25-37 ° C to release the microalgae released by the enzyme decomposition cell wall 谷物,作用時間24-48小時,使其最終酸驗值介於4.〇_4 2之間。 本發明的產物可進-步以儒乾燥機處理,依需要被製成各 種適合的劑型,在此可舉出的例子包括粉劑、膠囊、錠片、飲品, 但不僅限於此。 上述益生菌株,包括乳酸桿菌屬、鏈球菌屬 _麻⑽♦足球菌屬㈣⑽—、明串球菌屬仏__㈣ 和雙歧桿菌屬(Mycobacterium)等。 、上述益生_株可以單獨使用,也可以將不同種或不同株之 k生菌株做任何結合。 上述酵素,包括_分解_、多_分卿素、殿粉分解 酵素、或综合性酵素等。 上述微麵植物,包括螺旋藻、賴、紅藻、裸藻、金藻、 輪藻、異鞭藻等。 找最終微_馳分聽可進—料除㈣步驟處理,該 去除困體的步驟並娜m制,可舉_仔包括了離心及過滤。 200938626 破壁及有效成分單元化之二階段製程。 則述第-階段酵素分解之製程,係指利用酵素於25_37〇c作用 微藤類植物,依選用微藤類植物細胞壁成分不同而添加適當酵 素,作用時間18-24小時。 别述第一階段生物性伽之製程,係指利用活化之益生菌菌 株於25-37。(:作祕_素分解_雜_雛騎釋放之内 容物,作用時間24-48小時,使其最終酸驗值介於4 〇_4 2之間。 ❹ 本賴誠物可進-步以儒賴機處理,依需要被製成各 種適合的劑型,在此可舉出的例子包括粉劑、膠囊、鍵片、飲品, 但不僅限於此。 上述益生菌株,包括乳酸桿菌屬細論挪㈣、鍵球菌屬 作卿__)、足球菌屬的伽⑺靖」、明串球菌屬和腳 和雙故桿菌屬(gdobactefium)等。 “上述益生㈣株可以單獨伽,也可⑽不囉或不同株之 © 益生菌株做任何結合。 上述酵素’包括纖維分㈣素、多醣類分解酵素、澱粉分解 酵素、或综合性酵素等。 上述微藻類植物,包括螺旋藻、綠蒸、紅藤、裸簾、金藻、 輪藻、異鞭藻等。 上述最終微藻類植物分解液可進一步去除菌體步驟處理,該 除菌體的步驟並無_限制,可舉出的例子包括了離心及過滤。 7 200938626 【實施方式】 本發明第-階段先將微藻類植物以等比例 2广恆溫狀態和適當比例酵素混合均勻,作用18二:’於 =微_植滅合液,此為_植物_分解之第—= #又製程。將第一階段之酵素微藻 人 白 與高滚度益生賴株於25奶二,以姑1:6比例 酵槽培養24_48小時,當酸鹼 Ο 、、古4Ό左右時’即分解完成。细此微藻雛物之分解產物, 二w子量的賦糊,㈣霧乾燥機處理,製册末固態劑型 或各種適合的劑型。 蟾 200938626 【實施方式】 Ο 本發明第-階段先將微藻類植細等比例和純水混合,於 25抓怪溫狀態和適當比例酵素混合均勻,作用像Μ小時,形 成酵素微細植物混合液,此為__物細胞壁分解之第一階 段製程。將第-階段之酵素微_植物混合液,叫當Μ比例 與高濃妓生_株於25_3rC發酵槽培養沐48小時,當酸驗 值落在4.0左右時,即分解完成。利用此微_植物之分解^物, 齡高分子量_湖,讨魏軸處理,製成縣_劑型 或各種適合的劑型。 ‘ 4 〇 200938626 五、中文發明摘要: 本發嶋鶴嶋她_,㈣㈣ ❹ 方法進行織雜物細較錢後齡單元蚊二階段製程。 微藻馳物(例如:螺旋藻、綠藻、紅藻)經㈣-階段酵素進行 細胞破壁》解反應’合併高濃度益生菌進*將鶴類細胞破壁後 釋出的物加时解為小分子。此發明可提高微義植物的附 加價值、促進人體消化吸收率、降低微_的魚厘臭味、減低成 本等效益;所有的製程均在25_3rc下進行,不需要經過高溫高 壓耗費能源成本的製造單元程序。 六、英文發明摘要: This invention is aimed to add the economic value of algae (i.e. spiral, green, and red algae). It increases digestion & absorption of human gastrointestinal tract, improves the stinky fish flavor, and lowers the cost. There are two steps in the invention including: (1) ❹ destroying the cell wall of algae through enzyme reaction to release the cytosolic nutrients; (2) decomposing the larger cytosolic nutrients into smaller molecules through probiotics to promote human digestion and absorption. No energy is needed and all process is under 25-37°C. 200938626 五、中文發明摘要: 本發明係指微_植物分解之製程,_ 方法進行__錄細驗壁 I併生祕 微藻類植物(例如:螺旋_疋之—階段製程。 給&縣八* '、'、、、、杂藻、紅藻)經由第一階段酵素進行 、、、田=反應,合併高濃度益生菌_將__胞破壁後 釋出的内讀加以分解為小分子。此發明可提高鶴類植物的附 加價值、贱人體消化狄率、降低鶴_纽臭味、減低成 本等效益;财的製綱在25_37χ下進行,^需要經過高溫高 壓耗費能源成本的製造單元程序。 六、英文發明摘要: This invention is aimed to add the economic value of algae (i.e. spiral, green, and red algae). It increases digestion & absorption of human gastrointestinal tract, improves the stinky fish flavor, and lowers the cost. There are two steps in the invention including: (1) destroying the cell wall of algae through enzyme reaction to release the cytosolic nutrients; (2) decomposing the larger cytosolic nutrients into smaller molecules through probiotics to promote human digestion and absorption. No energy is needed and all process is under 25-37°C. 3 200938626 、申請專利範圍: ..本發明如旨縣雜物分解 法進行微 ^细酵素合併生物性方 階段製 尤棊類植物細胞破壁及有效成分單元化之 2_如申專利範圍第]The grain, the action time is 24-48 hours, so that the final acid value is between 4.〇_4 2 . The product of the present invention can be further processed by a drier dryer, and can be prepared into various suitable dosage forms as needed, and examples thereof include powders, capsules, tablets, and drinks, but are not limited thereto. The above probiotic strains include Lactobacillus, Streptococcus _ hemp (10) ♦ Soccer genus (4) (10), Stemococcus genus 仏__ (4) and Mycobacterium (Mycobacterium). The above probiotic strains may be used alone or in combination with k strains of different species or different strains. The above enzymes include _ decomposition _, _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ The above micro-planar plants include spirulina, Lai, red algae, Euglena, gold algae, Chara, Isochrysis, and the like. Looking for the final micro-sports can be read in. In addition to (4) step processing, the step of removing the trapped body and the system, can include centrifugation and filtration. 200938626 Two-stage process of breaking the wall and unitizing active ingredients. The process of the first-stage enzyme decomposition refers to the use of enzymes to act on 25_37〇c for micro-vines, and the appropriate enzyme is added according to the different cell wall components of the micro-vines, and the action time is 18-24 hours. The first stage of the biological gamma process refers to the use of activated probiotic strains at 25-37. (: Secret _ prime decomposition _ miscellaneous _ chick release content, the action time is 24-48 hours, so that the final acid test value is between 4 〇 _4 2 ❹ 赖 赖 诚 诚 诚The Ruyi machine is processed into various suitable dosage forms as needed, and examples thereof include powders, capsules, key sheets, and drinks, but are not limited thereto. The above probiotic strains include Lactobacillus genus (4), Staphylococcus is __), gamma (7) Jing of the genus Staphylococcus, genus Streptococcus and foot and gdobactefium. "The above probiotic (4) strains can be germinated individually or in combination with (10) non-producing or different strains of probiotic strains. The above enzymes include fiber fractions, polysaccharides, amylolytic enzymes, or synthetic enzymes. The above microalgae plant, including spirulina, green steamed, red vine, naked blind, gold algae, Chara, Isochrysis, etc. The final microalgae decomposition liquid can further remove the bacterial step, the step of removing the bacteria There is no limitation, and examples include centrifugal and filtration. 7 200938626 [Embodiment] In the first stage of the present invention, the microalgae plants are uniformly mixed in an equal ratio of 2 constant temperature and an appropriate ratio of enzymes, and the effect is 18: '于=微_植灭合液, this is the _ plant_decomposition of the first -= #又process. The first stage of the enzyme microalgae human white and high rolling probiotics in the 25 milk two, to aunt 1: 6 ratio fermentation tank culture for 24_48 hours, when the acid and alkali Ο,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,, Solid dosage form or various剂200938626 [Embodiment] Ο In the first stage of the invention, the microalgae plant is mixed with pure water and mixed with pure water, and the mixture is uniformly mixed with the appropriate proportion of enzymes in the temperature state of 25, and the effect is like Μ hour, forming a fine enzyme. Plant mixture, this is the first stage of the cell wall decomposition of the __. The first stage of the enzyme micro-plant mixture, called the sputum ratio and the high concentration of sorghum _ strain in the 25_3rC fermentation tank for 48 hours, when the acid When the test value falls at about 4.0, the decomposition is completed. Using this micro-plant decomposition product, the age-high molecular weight _ lake, and the Wei axis treatment, the county _ dosage form or various suitable dosage forms. ' 4 〇200938626 V. Abstract: This hairpin 嶋 嶋 her _, (4) (4) ❹ Method for the second phase of the groceries and fines after the unit of the unit mosquito. Microalgae (such as: spirulina, green algae, red algae) by (four)-stage enzyme The cell wall breaking reaction is combined with the high concentration of probiotics. The material released after the cell wall is broken is added to the small molecule. This invention can increase the added value of the micro-sense plant and promote digestion and absorption. Rate, reduce the odour of micro-fish, reduce cost and other benefits; all processes are carried out at 25_3rc, without the need for high-temperature and high-pressure energy cost manufacturing unit procedures. VI. English Abstract: This invention is aimed to add The increased value of algae (ie spiral, green, and red algae). It increases digestion & absorption of human gastrointestinal tract, improves the stinky fish flavor, and lowers the cost. There are two steps in the invention including: (1) ❹ destroying the cell wall of algae through enzyme reaction to release the cytosolic nutrients; (2) decomposing the larger cytosolic nutrients into smaller molecules through probiotics to promote human digestion and absorption. No energy is needed and all process is under 25-37°C 200938626 V. Abstract of the Invention: The present invention refers to the process of micro-plant decomposition, _ method for __ recording the wall I and secret microalgae plants (for example: spiral _ 疋 - stage process. Give & County VIII*, ', ',,, algae, red algae through the first stage of enzymes, and, field = reaction, combined with high concentration of probiotics _ will be released after __ cell wall Decompose into small molecules. The invention can improve the added value of the crane plant, the digestibility rate of the human body, reduce the odor of the crane, and reduce the cost of the crane; the financial system is carried out under 25_37, and the manufacturing unit program requires high temperature and high pressure to consume energy costs. . 6. The invention is targeted to add the economic value of algae (ie spiral, green, and red algae). It increases digestion & absorption of human gastrointestinal tract, improves the stinky fish flavor, and lowers the cost. There are two steps in the invention including: (1) destroying the cell wall of algae through enzyme reaction to release the cytosolic nutrients; (2) decomposing the larger cytosolic nutrients into smaller molecules through probiotics to promote human digestion and absorption. 3 。 。 。 。 。 。 。 。 。 。 。 。 。 。 。 。 。 。 。 。 。 。 。 。 。 。 。 。 。 。 。 。 。 。 。 。 。 。 。 。 。 。 。 。 。 。 。 。 。 。 。 。 。 Ingredient unitization 2_such as the patent scope] 合性酵素等 3::=第1項所述之第-階段酵素分解之製程,酵 ΙωΓ .1解酵素、多51類分解酵素、_分解酵素、综 4·如Μ專·_ 1撕叙第二階段生物性仙之製程, ,指利用高濃度益生菌株培養液發酵分解微藻類植物之内 各物,形成小分子單元體。 如申吻專她圍第彳項所述微藻馳物分解之製程,其最終 產物可進—步以去除_步驟處理,該去__步驟並無 特别限制’可舉出的例子包括了離^及過遽。 6·如申請專職_彳項所述繼食品之製程,產物可依需要 被製成各種適合的劑型,在此可舉出的例子包括粉劑、膠 囊、錠片、飲品,但不僅限於此。 如申咕專利範圍第4項所述之益生菌株包括乳酸桿菌屬 (Lactobacillus)、遂珠隹亀(strepi〇c〇ccus)、足像議爆 (TWzbcocc叫)' 明串球菌屬和和雙歧桿菌屬 200938626 十、申請專利範圍·· 1. 本發明係指微藻類植物分解之製程,利用酵素合併生物性方 法進行微藻類植物細胞破壁及有效成分單元化之二階段製 程。 \ 2. 如申请專利麵第】項所述之微藻類植物,係指螺旋藻、綠 藻、紅藻、裸藻、金藻、輪藻、異鞭藻等。 3. 如申凊專利細第彳項所述之第—階段酵素分解之製程,酵 素係指纖雄分解酵素、多酶類分解酵素、澱粉分解酵素、綜 合性酵素等。 4_如申請專利顧第1項所述之第二階段生物性侧之製程, 係指利用高濃度益生g株培養液發酵分解微_植物之内 容物,形成小分子單元體。 5. 如申明專槪H第1撕述微細植物分解之製程,其最終 產物可進—步以去_體步驟處理,該去除菌體的步驟並無 特別限制,可舉出的例子包括了離心及過渡。 · 6. 如申請專利範圍第1項所述健康食品之製程,產物可依需要 被製成各種適合的劑型,在此可舉出的例子包括粉劑、膠 囊、錠片、飲品,但不僅限於此。 7. 如申請專利範圍第4項所述之益生師包括乳酸桿 知伽、鏈球菌屬〜__、足球菌屬 (Pe^ococcus)、明电捸玆蘧 甲衣菌屬和雙歧桿菌屬 200938626 等。所使用菌株最好為嗜酸乳桿菌 (Lactobacillus acidophilus)、乾酷·乳得議(Lactobacillus 、保加利亞菌^、嗜熱乳桿菌 (Streptococcus thermophilus)、乳珠議(Lactococcus lactis)。Jl 述益生菌菌株可以單獨使用,也可以將不同種或不同株之乳 酸菌做任何結合。Synthetic enzymes, etc. 3::=The process of the first-stage enzyme decomposition described in Item 1, the yeast Ι Γ 1 .1 lyase, 51 kinds of enzymes, _decomposing enzymes, comprehensive 4·如Μ专·_ 1 tearing The second stage of the biological fairy process, refers to the use of high concentration probiotic strain culture solution to ferment and decompose the microalgae plants to form small molecular units. For example, Shenkun specializes in the process of decomposition of microalgae, which is described in the third item, and the final product can be further processed to remove the step processing. The step of the __ is not particularly limited. ^ and too. 6. If the food processing is carried out as described in the full-time _ item, the product may be prepared into various suitable dosage forms as needed, and examples thereof include powders, capsules, tablets, and drinks, but are not limited thereto. Probiotic strains as described in item 4 of the scope of the patent application include Lactobacillus, strepi〇c〇ccus, pedicle bombardment (TWzbcocc), genus Streptococcus and bifid Bacillus 200938626 X. Patent Application Scope 1. The present invention refers to a process for decomposing microalgae plants, and a two-stage process for microalgae plant cell breaking and unitization of active components by using enzyme and biological methods. 2. 2. Microalgae plants as described in the application for patents refers to spirulina, green algae, red algae, euglena, gold algae, spirulina, and whipsy. 3. For the process of enzyme decomposition in the first stage of the application of the patent, the enzyme refers to the enzyme decomposing enzyme, multi-enzyme decomposing enzyme, amylolytic enzyme, and comprehensive enzyme. 4_ The second-stage biological side process as described in the application of the patent application refers to the use of a high concentration of probiotic g-culture medium to ferment and decompose the contents of the micro-plants to form small molecule units. 5. If the process of decomposing the micro-plant decomposition is specified, the final product can be further processed in a step of removing the cells. The step of removing the cells is not particularly limited, and examples include centrifugation. And the transition. · 6. If the process of the health food described in the first paragraph of the patent application is applied, the product may be prepared into various suitable dosage forms as needed, and examples thereof include powders, capsules, tablets, drinks, but not limited thereto. . 7. Probiotics as described in item 4 of the scope of patent application include lactate gamma, streptococci ~__, Pe^ococcus, scutellaria, and Bifidobacterium 200938626 Wait. The strain to be used is preferably Lactobacillus acidophilus, Lactobacillus, Bulgarian bacteria, Streptococcus thermophilus, Lactococcus lactis. Jl probiotic strain It can be used alone or in combination with lactic acid bacteria of different or different strains. 8.如申請專利範圍第4項所述高漢度益生菌株培養液,係指利 用·^生菌株共同培養於營養基質内,營養基質包括奶類、豆 漿、茶等。上述奶類並無特別限制,可為多鶴物所產之乳 水也可為還原乳。動物所產的乳水之可舉出的例子包括牛 ^和=奶,較佳者為牛奶。上述豆漿並無制關,可為黃 '黑丑、h、花豆、敍等。上述茶類並無特別限制, 可為紅余、綠茶、烏龍茶、桂花茶、藥草茶等。 A 範圍第4項所述高濃度益生—,縣 曰田基、或表面活性劑。 1。:、:==9:之製程,_'糖、乳 11·如申請專利範圍第 葡聚糖、植物膠、阿拉伯膠等。各防相包括海藻糖、 如申4利鋼第9項所述之製程,抗氧化劑包括维生素 200938626 等。所使用菌株最好為嗜酸乳桿菌 (Lactobacillus acidophilus)、氡絡% 桿鸷(Lactobacillus cos匈、保加利亞菌江、嗜熱乳桿菌 印tococc批 i/iermop/n7_、乳球菌(Xaciococcw>s ⑹叫。上 述益生菌菌株可以單獨使用,也可以將不同種或不同株之乳 酸菌做任何結合。 8. 如申請專利範圍第4項所述高濃度益生菌株培養液,係指利 用益生菌株共同培養於營養基質内,營養基質包括奶類、豆 漿、茶等。上述奶類並無特別限制,可為多種動物所產之乳 水’也可為還原乳。動物所產的乳水之可舉出的例子包括丰 奶和羊奶,較佳者為牛奶。上述豆漿並無特別限制,可為黃 一 ”、、豆、.工豆、花豆、米豆等。上述茶類並無特別限制, 可為紅茶、綠茶、烏龍茶、桂花茶、藥草茶等。 φ 9. 如申請專利範圍第4項所述高濃度益生菌株培養液中,可添 加充填劑、防_、抗氧化劑、PH調整劑、pH緩衝劑、除 自由基、或表面活性劑。 ’、 10. 如申請專利範圍第9項所 糖、脱脂奶粉'山梨醇等製程’充填劑包括廉糖、乳 防凍劑包括海藻糖、 抗氧化劑包括维生素 11. 如申請專利範圍第9項所述之製程, 葡聚糖、植物膠、阿拉伯膠等。 12. 如申請專利範圍第9項所述之製程, 11 200938626 C、维生素E、氨基酸等。 13. 如申請專利範圍第9項所述之製程,pH調整劑包括碳酸氫 納、氫氧化納等。 14. 如申請專利範圍第9項所述之製程,pH緩衝劑包括磷酸缓 衝溶液等。 15. 如申請專利範圍第9項所述之製程,除自由基劑包括綠茶、 卢胡蘿蔔素、茄紅素(lycopene)等。 16. 如申請專利範圍第9項所述之製程,表面活性劑包括卵雄 脂、蔗糖脂肪酸酯等。 200938626 C、维生素E、氨基酸等。 13. 如申請專利範圍第9項所述之製程,pH調整劑包括碳酸氫 納、氫氧化鈉等。 14. 如申請專利範圍第9項所述之製程,pH緩衝劑包括磷酸緩 衝溶液等。 15. 如申請專利範圍第9項所述之製程,除自由基劑包括綠茶、 冷胡蘿蔔素、茄紅素(lycopene)等。 16·如申請專利範圍第9項所述之製程,表面活性劑包括卵磷 脂、蔗糖脂肪酸酯等。 ❹ 128. The high-yield probiotic strain culture solution according to item 4 of the patent application scope refers to the use of the strain to co-cultivate the nutrient substrate, and the nutrient substrate includes milk, soybean milk, tea and the like. The above-mentioned milk is not particularly limited, and may be a milk produced by a plurality of cranes or a reduced milk. Examples of the milk produced by the animal include bovine and milk, preferably milk. The above-mentioned soybean milk has no control, and it can be yellow 'black ugly, h, flower bean, Syrian and so on. The above teas are not particularly limited and may be red, green tea, oolong tea, osmanthus tea, herbal tea, and the like. A range of high concentration probiotics mentioned in item 4, county 曰田基, or surfactant. 1. :, :==9: The process, _ 'sugar, milk 11 · such as the patent scope of the first glucan, vegetable gum, gum arabic and so on. Each phase includes trehalose, such as the process described in item 9 of Shenli Steel, and antioxidants include vitamin 200938626. The strains used are preferably Lactobacillus acidophilus, Lactobacillus cos Hungary, Bulgarian bacteria, Lactobacillus thermophilus tococc batch i/iermop/n7_, Lactococcus (Xaciococcw>s (6) The above probiotic strains may be used alone or in combination with lactic acid bacteria of different species or different strains. 8. The high concentration probiotic strain culture solution described in the fourth paragraph of the patent application refers to the use of probiotic strains for co-cultivation in nutrition. In the matrix, the nutrient substrate includes milk, soy milk, tea, etc. The above-mentioned milk is not particularly limited, and may be milk produced by various animals, and may also be reduced milk. Examples of milk produced by animals may be mentioned. Including milk and goat milk, preferably milk. The above-mentioned soy milk is not particularly limited, and may be yellow one, beans, beans, beans, rice beans, etc. The teas are not particularly limited, but Black tea, green tea, oolong tea, osmanthus tea, herbal tea, etc. φ 9. Adding filler, anti-oxidant, pH to the high-concentration probiotic strain culture solution as described in item 4 of the patent application scope Agent, pH buffer, free radical, or surfactant. ', 10. If the sugar in the ninth paragraph of the patent application, the process of skimmed milk powder 'sorbitol', including the low-sugar, milk antifreeze including trehalose, The antioxidant includes vitamin 11. The process described in claim 9 of the patent scope, dextran, vegetable gum, gum arabic, etc. 12. The process described in claim 9 of the patent scope, 11 200938626 C, vitamin E, Amino acid, etc. 13. The process as described in claim 9 of the patent application, the pH adjusting agent comprises sodium hydrogencarbonate, sodium hydroxide, etc. 14. The pH buffer comprises phosphoric acid as described in the scope of claim 9 A solution such as a solution. 15. The process described in claim 9 includes free radicals including green tea, caroterin, lycopene, etc. 16. The process described in claim 9 Surfactants include egg and male fat, sucrose fatty acid ester, etc. 200938626 C, vitamin E, amino acid, etc. 13. As described in the scope of claim 9, the pH adjusting agent includes sodium hydrogencarbonate, hydrogen hydroxide 14. The process of claim 9, wherein the pH buffer comprises a phosphate buffer solution, etc. 15. The process of claim 9, wherein the free radical agent comprises green tea, cold carotene, Lycopene, etc. 16. As described in the scope of claim 9, the surfactant includes lecithin, sucrose fatty acid ester, etc. ❹ 12
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