TW200906431A - Polygonum multiflorum extract capable of improving metabolic syndrome, pharmaceutical and food compositions thereof - Google Patents

Abstract

The present invention provides a Polygonum multiflorum (Taiwan Tuber Fleeceflower) extract capable of improving metabolic syndrome, pharmaceutical and food compositions thereof. The present invention discloses a Polygonum multiflorum extract capable of improving the metabolic syndrome. The present invention also discloses a pharmaceutical composition containing Polygonum multiflorum extract for improving the metabolic syndrome. The present invention further discloses a food composition containing Polygonum multiflorum extract suitable for patients with the metabolic syndrome. A simple preparation method is applied in the present invention to produce the invented extract and compositions, and it is verified that the invented extract and compositions have the functions of regulating blood-sugar and blood-lipid , inhibiting the activities of glucosidase and acetyl-CoA carboxylase (ACC), as well as promoting the activities of acyl-CoA oxidase (ACO). Therefore, the present invention only needs to use a single Polygonum multiflorum extract to achieve the effect of improving the metabolic syndrome.

Description

200906431 IX. Description of the Invention: [Technical Field of the Invention] The present invention relates to a red pheasant extract, in particular to a saccharide-reducing enzyme (aCety-CoA carboxylase) capable of inhibiting intestinal glycolytic enzymes and liver. , ACC), and promotes the activity of 醯Kease A oxidase (AC0) in the liver to improve the metabolic syndrome of the red chicken vine extract. [Prior Art]

U.S. Patent No. 5,531,991 discloses a "Composition and Method for Treating Hyperglycemia Using Heshouliang Extract", which is an extract obtained by extracting Polygonum multiflorum with ammonium hydroxide of 〇.彳N through a chlorinated gel. G_25 (Sephadex g_25) is divided into three fractions, and the extract of Heshou has similar effects of increasing insulin activity and regulating blood sugar. In the U.S. Patent No. 6,200,569, a "composition and method for increasing insulin activity" is disclosed, which is a kind of bacteria or silver leaf using He Shouliang (10) (7) and the agaricaceae (4) (4) The compound water extract of the compound water (C丨nnamomum mairei) has the effect of increasing insulin activity and has the function of regulating blood sugar. However, the above two US patents use the Polygonum multiflorum (4) to do _ miV/ i//7〇R/n7) is used as a raw material, and the blood sugar is adjusted by the mechanism of 掸_s plus insulin activity. Therefore, it does not have the effect of the blood wall of the right wall, and it does not improve the metabolic syndrome. efficacy. ^八通200906431 Further, in the patent of EP1 82951 9A1, a "pharmaceutical composition for treating and/or preventing hyperlipidemia and a method for producing and using the same," disclosed by Ho Shouwu, Shan I. The extract of Danqi and Shanqi, a compound composition consisting of - a proportional composition, which has a function of regulating blood lipids. This result is only presented in the human test results' and does not reveal a possible mechanism of action. However, the above-mentioned European patent utilizes Polygonum multiflorum as a raw material, and the compound composition which must be formed with other extracts has a function of regulating blood lipids, but it does not disclose the effect of regulating blood sugar or improving generation. - In summary, the existing composition of reducing gold or lowering blood fat is utilized: the traditional Chinese Polygonum multiflorum (P. (four). _ m / screaming, its origin in the mainland of the country' and the traditional Polygonum The medicinal part lies in its hypertrophic block: the scorpion sees only single-functions such as hypolipidemic or hypoglycemic on the market: or sputum, there is no drug or food for metabolic syndrome. Deficiency: Xie syndrome occurs The situation is more and more, so this study is to inhibit the absorption of intestinal sugar, and (4) (4) Guan:: oxidized phytoplasma 'material' to develop a solution to the metabolic syndrome Γ 【Abstract】 Wu Youxin at the present The use of Polygonum multiflorum is the traditional Heshou I only to reduce the blood sugar or blood loss of the purpose: the ability to study the metabolism of metabolic disease materials.,,, any U after a long period of research 200906431 and constant attempts After that, the extract of the red pheasant has been invented to improve the function of the metabolic syndrome. The object of the present invention is to provide an extract which utilizes the red pheasant (also known as Polygonum multiflorum) to inhibit intestinal glycolytic solution. yeast And the activity of Escherichia coenzyme (ACC) in the liver, and sub-promoting the activity of the 醯SK enzyme octadecylase (acy|_c〇A AC0) in the liver for improving metabolic syndrome. Red chicken vine vine extract. 'To achieve the above object, the present invention relates to a red pain vine extract for improving metabolic syndrome', which comprises the following method: providing fresh or dried red chicken vine a plant or a part; extracting the red hen or the vine from the solvent to obtain a solvent extract; concentrating and drying the (four) extract; and obtaining the red pheasant extract. Preferably, the red chicken vine is provided locally. Preferably, the solvent for extracting the red chicken #vine plant or the local solvent may be water, an alcohol, an aqueous alcohol solution, and an acetic acid, wherein the water is still red at a temperature of ascending ancient For the vine plant or partial extraction, the system can be used to treat the medicinal pot of the cockroach. The cockroach is directly added to the vine plant or the vine plant is added... It is better that the solvent is in the concentration of &~_wt% pure Ethanol or (10) aqueous solution 'best The concentration of ethanol is 7Q~(10); the best β-fermentary solvent is acidic alcohol containing hydrochloric acid. '疋 It is better to extract the red-skinned stem and the roots using the acid essence' in the weight of the red chicken With an acid alcohol volume of about 1:5 to "n / case, at room temperature. 'The present invention is related to a pharmaceutical combination for improving metabolic syndrome 200906431 Chicken vine extract and pharmaceutically acceptable The carrier or the shaped "roughing" of the red chicken vine extract includes the steps of: providing fresh or simmered red chicken vine plants or a plant or a part to obtain a solvent extract. Mixing the red chicken raw vines to take the red chicken _ extract; and taking the red extract; receiving the carrier or excipient into the doctor's sputum 2: the stroke "additional medicine can be used. 4% of the composition, in order to improve the metabolic syndrome: the best is the red stem of the vine stems and roots. The water, m, and the solvent for extracting the red vine or the partial vine can be a bis-alcohol aqueous solution and an ethyl acetate vinegar, wherein the water can still be raised by a human:::::: red sorghum plant or a partial extraction, the system The water can be placed in a warmer, and the 'Kezhi medicine pot' directly adds the red chicken Moto plant or the local ethanol to the solvent to a concentration of 25, _% pure ethanol or B% water-cooled liquid, the best The ethanol concentration is 70 to 100 w. The alcohol solvent is an acidic alcohol containing hydrochloric acid. , the best 疋 '精:::: 2: Take the red chicken Moss stem and roots using acid wine = (four) win weight and acid alcohol volume is about 1:5 to dry and dry, at room temperature get on. The composition is related to a fresh or extracted food group suitable for patients with metabolic syndrome, and the preparation steps include: obtaining solvent extract from the extract; drying and drying the solvent extract * Good generation:::::: Stroke; And the food composition is changed to 200906431. Preferably, the red vine is provided with a stem and a root. Preferably, the solvent for extracting the red vine or the local vine is water, alcohol, An aqueous alcohol solution and acetic acid §|, wherein the water can be subjected to extraction or local extraction of the red pheasant plant at an elevated temperature, and the water can be placed in a medicine pot of a general medicinal Chinese medicine, directly red (4) The vine plant is locally warmed and boiled; more preferably, the solvent is a pure ethanol or an aqueous alcohol solution having a concentration of 25 wt%, and the optimum concentration of the yeast is 7〇~1〇_%; optimally, the alcohol The solvent is an acid alcohol containing hydrochloric acid. It is preferred to extract the red (four) cane and roots using acidic alcohol, and the ratio of the weight of the red chicken vine to the acid alcohol is about 1:5 to 1:2 〇. Within the range 'at room temperature.

Since the steps of the present invention are simple, and the effective energy red vine extract can be extracted without special solvent, the cost is low, and the glycolytic enzyme and the liver sputum coenzyme A m enzyme (Acety|_c) are in the intestinal tract. 〇A carb〇xyiase, ACC) was tested* to confirm the effect of (iv) and to promote the activity of sulfhydryl-A oxidase (AGy|_CQA(10)d, ACO) in the liver. Therefore, the present invention uses only _ The red vine extract can simultaneously achieve the function of regulating blood lipids and regulating blood sugar, and has the effect of improving metabolic syndrome. [Embodiment] Please refer to the figure - the present invention relates to a red vine extract for use in Xie syndrome, which is prepared by the following method, including providing fresh or dried red chicken or vine plants or Partially (8); extracting the red "vine plant or part by solvent to obtain solvent extract (b); concentration 9 200906431 drying the solvent extract (c); and obtaining after preparation of the extract containing the red worm Taking the food composition containing the red chicken «the food composition of the extract = (4) or the invention of the invention first extracts fresh or strain with a solvent, and uses a solvent to red chicken ears and rattan The active ingredient is dissolved, and further: =: soluble in the solvent of Shiba 7 and 6 kinds of active 2 discs soluble in the solvent to treat intestinal glycolytic enzymes and enzymes in the liver, found that: sugar And blood lipids regulate key enzyme activity. In a specific embodiment of the present invention, it can be found that the use of the soluble active ingredient has the activity of inhibiting intestinal glycolytic enzyme, liver acetaminophen-A (tetra) enzyme, and promoting hepatocytes Stuffed A-oxidizing enzyme Therefore, the red chicken #vine extract obtained by the present invention can be further compounded with a pharmaceutically acceptable carrier or excipient to make a medical combination for treating metabolic syndrome (4) related health foods, etc. The invention refers to the "Red Chicken Pingteng", also known as Taiwan's Polygonum (Taiwan Tuber F|eeceflower). Its origin is Taiwan, which is an intrinsic species of Taiwan. It does not have obvious roots. It is a variant of the traditional Polygonum multiflorum. It is also a Chinese herbal medicine. One kind is common in the middle and low-altitude mountainous areas of Taiwan, and grows mostly on the roadside and forest edge. However, no one has replaced the roots of Taiwanese Polygonum multiflorum in the traditional Polygonum multiflorum. The red chicken is a genus of genus and genus (P 〇/yg〇A7aCeae) 'The scientific name can be Po/yg〇A?i7/7? _resistant/〇/__

Thunb. Ex murray var. hypoleucum (Ohwi) Liu, Ying & Lai 'Pleuropterus hypoleucus multiflorum, Polygonum hypoleucum Ohwi and Polygonum multiflorum Thunb. var. 200906431 (〇hw丨)Liu,Ymg & La Buchigen and vine (stem) Part of the sentence of antitussive / · go to the wind, 怯痰 'treatment of cold, cough, rheumatism, etc., and the leaf can treat colds, external wounds and so on. The term "metab〇nc syndr〇me" as used by Ben Maoming means 姨 ance 阻抗 impedance syndrome (heart esistance syndrome), which is associated with a type 2 diabetes patient with a heart-shaped obesity High, fat and other symptoms. Insulin resistance (insunn "esist_e" should be the common pathogenic turnaround of this cluster] can inhibit the intestinal sugar =, inhibit the liver acetaminophen coenzyme A, promote the liver Kiev gasification enzyme, blood sugar control, blood lipid concentration is improved. Chicken shore = hair and the "red chicken raw vine extract" means the use of solvent to extract red = plant to obtain red chicken raw vine soluble solvent The solvent may be water, an alcohol such as ethanol, an aqueous alcohol solution, and a thick vine: preferably, it is an acidic alcohol. In the present invention, the red chicken or the dried whole plant or part; The chicken broiler, the cane or the red vine root, etc.; in the hairline, the red pheasant stems and the roots of the stalks. The F-precipitation β π Kang drying method and the second line of the present invention can be concentrated under reduced pressure. Method, cold drying method. Generally known to the knowledge of the concentrated dry copy of the word "medicinally acceptable carrier, bucket - oral type, P, Yu Gong", right medicine combination position is not protected before the pharmaceutical composition in It has not yet reached the decomposition of 4 chemistries, but the release of the drug and the effect of the drug. Use, and / or can be 11 200906431 - medicinal acceptable excipients for the drug is the uniformity of the drug, _ ' In order to increase the stability and reduce the irritancy of the drug, the bad alcohol, the powder or other two-group extracts known to those of ordinary skill in the art, the system can be made into an oral administration form for improving metabolic syndrome: =τ The different forms are included, including but not limited to large, including the novel novel form of red hen, vine extract: two acceptable carriers, and the form for oral administration may be selected from the group consisting of the agent 1, the suspension, the chyle The liquid, the capsule, the granule, the tablet, the liquid, the alcohol, and the medicated sugar are destroyed. ' The following examples are for the purpose of specifically illustrating the technical means of the present invention = the red material of the present invention and Specific material action活性 Κ Κ Κ Κ Κ Κ Κ Κ 制备 制备 制备 制备 制备 制备 制备 制备 制备 制备 制备 制备 制备 制备 制备 制备 制备 制备 制备 制备 制备 制备 制备 制备 制备 制备 制备 制备 制备 制备 制备 制备 制备 制备 制备 制备 制备 制备 制备 制备 制备 制备The ratio of the weight of the red vine to the acid alcohol (4) is 1:8 (W/V = 1/8), and it is placed at room temperature and shaken at a speed of 1000 revolutions (rpm). After s σ 72 hours, the initial (4) is made with gauze, and the obtained (four) liquid is then cut (each at a rate of 15 minutes, and the upper layer is concentrated and dried to become an acidic alcohol extract) with an extraction rate of about 6 9%. The extract is reconstituted to a concentration of 5 〇mg/m 丨. 12 200906431 The hot water extract is put into the Chinese medicine pot for about two hours, and the weight of the red chicken vine is The volume ratio of water is about 1:20 (W/V = 1/20), and it is filtered with gauze. The filtrate is freeze-dried to obtain a hot water extract with an extraction rate of about 9.2%. Reconstitution, the concentration was 50 mg/m b as the acidic alcohol extract to carry out the following experiment. Example 2 The extract of E. sinensis inhibits α-glucosidase (α · glucosidase ) -D-glucoside (p-nitrophenyl a-D-glucoside, pNP-G) was analyzed by mass spectrometry to inhibit α-glucosidase activity. The method includes: 1. Firstly, scallop buffer solution (1 mM) , pH 7.0) Configure 1 U/ml α-glucosidase (EC 3.2.1.20, G5003, Sigma-Aid rich Co., USA) solution and 5x1 _3M ^ .~ nitrophenyl glucopyranoside (p_ nitrophenyl a -D-glucopyranoside, pNP-G, Sigma-Aldrich

Co., USA) The solution was taken up. 2 Subsequently, 4 μL of microliter (p丨) phosphate buffer solution (0_4 M, pH 7_0) and 1 μL of micro-liter (//丨) α-glucosidase solution were added to each well of a 96-well plate. , and 5 liters of red broth extract ("丨) of different dilutions, add deionized water to 170 μl ("丨). 3. Allow the incubator to warm up in an environment of 37 ° C for 5 minutes, then add 3 〇 u liter (/ / 丨) preheated texture solution, after sufficient shaking and mixing, in the environment of 37 13 200906431 °c The next action is ι 〇 minutes. 4. Record the absorbance values before and after the wavelength of the nanometer (nm) photometric analyzer (ρ. lamp, Bi〇〇TekC〇., USA), and Go means that the P system 50 / α α _ glucoside The concentration required for enzyme activity. Refer to Table 1 for the description of the acidic alcohol extract and hot water extract. The results obtained are: lc5 (p (extract A/q/ml) - acidic alcohol extract acid alcohol extract hot water extract #1 取物叹-5·5±〇·5 6.5 + 0^7^ ”7_1±〇·4 Fine Table 1 shows the acidic alcohol and hot water extract of red chicken vines for α-glucosidase The activity has an inhibitory effect, and the effect obtained by the acidic alcohol extract is better. The application example 3. The inhibition of the activity of different glycolytic enzymes by the extract of the red pheasant vines~ The crude extract of the intestinal enzyme of the rat is used. (rat jntestinai acet〇ne powder, Sigma Chemica, Co., St. Louis, source of different sugars (maltose, sucrose and starch) to test the acid alcohol red carp vine extract for different glycolytic enzymes The inhibition of activity. Please refer to Table 2, which shows the inhibition of the activity of different glycolytic enzyme activities by the use of acidic alcohol red chicken vine extract 14 200906431, which also uses 丨c 5〇 to represent Table 2 .

Table 2 above shows that the 'acidic alcohol extract for maltose sugar decomposing enzyme _丨tase), (four) glycolytic enzyme (α丨咖), and #, sugar glycolytic enzyme (su(10)e) have inhibitory effects, and the inhibitory strength is maltose. Glycolysis > Caramel Dehydrogenase > Sucrose Glucose Enzyme Solid yoke example 4 - Preparation of fat-soluble extract of red pheasant vines After grinding with red grinder, the extract was extracted with a concentration of 5 〇 wt% aqueous methanol, and the weight of red pheasant and 50% by weight of sterol The volume ratio of the aqueous solution was 1:10 (W/V=1/10), and the mixture was shaken and extracted at room temperature for 24 hours, and then the fabric was added to an equal volume of ethyl acetate/water. After shaking and mixing, liquid-liquid extraction is carried out, and the upper layer of ethyl acetate layer is taken, and then mixed with 5 wt. of sodium bicarbonate (NaHCO) in an equal volume of ethyl acetate, and then the sodium hydrogencarbonate layer is removed. After washing away the free fatty acid, and then concentrating and drying the ethyl acetate layer, the desired fat-soluble extract is obtained, and the extraction rate is about 〇·g 1 〇/〇. 15 200906431 The fat-soluble extract is dimethyl sulfoxide. (Djmethyl Sulfoxide, DMso) reconstituted to a concentration of 10 mg / m. Example 5 - inhibition of Escherichia coli AES with A. Activity analysis of acetaminophen coenzyme A carboxylase (ACC) is The key enzyme for fatty acid synthesis, its product, malony, is a receptor for fatty acid synthase (FAS), and is a carnitin palmitoyl CoA transferase (CPT). Inhibitors' thus inhibiting ACC not only inhibits endogenous lipid synthesis, but also accelerates oxidation of fatty acids in the mitochondria. ACC was purified from rat liver and tested in the same way as Tanabe et al. (Tanabe T., Nakanishi, S., Hashimoto T., Ogiwara, H., Nikawa J., Numa S. 1981. Acetyl-CoA carboxylase from rat liver, Meth. Enzymol. 71(pt C): 5--16). Table 3: Table 3. Percentage of inhibition of ACC activity (%) Acidic alcohol extract i_0.05mq/ml) 61.27 + 5.4 Fat-soluble extract (〇.〇5mg/ml) 45.99 + 1 1.9 16 200906431

The activity of the acidic alcohol and fat-soluble extract of red chicken vine. Then take the acidic alcohol extract - the figure is not 'the linear change of the red wine 屎 vine acid wine sub-fruit, so the figure can be seen that 50% of the ACC activity required red chicken vine vine acid alcohol extract promotes 醯 Kiev enzyme Activity analysis of oxidase (Acyl-CoA oxidase, ACO) is a key enzyme for the oxidation of fatty acids, and is also a typical peroxisome proliferator-activated receptor σ (ppARCT). Downstream genes can be used as indicators of fatty acid oxidative metabolic activity. This analysis is to inoculate H4丨丨EC3 hepatocytes in a 12-well plate at an appropriate density. After being attached, the cells are replaced with different concentrations of gas butyl ester (C|0fibrate) which are known to promote AC〇 performance. The micro-liter/ml (;c/|/m丨) red chicken vine vine acid alcohol extract was cultured for a suitable time, and the cells were collected by aspirating the culture solution, and the activity test was performed for AC0. The AC0 activity test is conducted by a scholar such as Small (Sma丨丨GM_ Burdett K. ConNock MJ. 1985. A sensitive spectrophotometric assay for peroxisomal acy CoA oxidase. 8/〇c/7em/ca/ J〇t//77a /· 227:205-210.) method. Please refer to the third figure, where the third (A) diagram shows the use of different concentrations (1 00//M, 500 #M, 1 ΟΟΟρΜ) of phenyl butyl vinegar at different times (48 and 72 hours) The degree of ACO activity can be promoted; and the third (Β) Figure 17 200906431 shows the fold increase in ACO activity after 48 hours of treatment with 1 〇M/ml of red chicken screen and vine acidic alcohol extract. Example 7. Effect of Red Chicken Screen 'Vine Acid Alcohol Extract on High Lipid Induced Metabolic Syndrome C57BL/6 J Mouse C57BL/6J Mice First High-fat Feed Silver with 3〇% (g/g) Cream Eight weeks of symptoms of metabolic syndrome such as obesity, hyperlipidemia, and hypertonic acid. The mice with symptoms of metabolic syndrome were arbitrarily divided into two groups, and one group was given a high-fat diet supplemented with 3% (g/g) of red chicken vine vine acidic alcohol extract (equivalent to 15 kg per kg of mouse body weight). 〇〇mg of red chicken vine vinegar acid alcohol extract (1500mg/kg body weight) (HF + No.11), the other group maintained high fat diet as the control group (HF). There is also a normal feed control group (LF). After five weeks of feeding, the glucose tolerance test (0GTT) was performed. The results were sacrificed in mice as shown in Table 4. The final body weight and blood biochemical analysis results are shown in Table 5. Table 4 Changes in blood glucose in mice of each group at the 5-week oral glucose tolerance test

Blood glucose (mmol/L) AUCion Omin 30min 60min 90min 1 20min (mmol*min/L) LF (n = 6.1±1. 10.37 soil 9.97±1. 8.75±1. 8.56±1. 656·3±93·3 a 14) 32a 1.45 a 25 a 52 a 55 a HF (n = 8.76 ± 12.27 ± 12.16 ± 12.25 ± 13.74 ± 961.2 ± 76.3C 18 200906431

Show. Superscripts with different letters The results are significantly different in the mean ± standard deviation (SD) table, p &lt; 〇, 0 5. Table 4,, „Members, 'Stars for five weeks with red chicken vine vine acid alcohol extract, can significantly reduce high-fat-induced metabolic syndrome C57BL/6m fasting blood glucose') and make it in oral glucose tolerance test 6 〇 and 9 〇 minutes of sugar and 2 small B under the blood sugar curve area (area Xie (10), auc) The monthly sputum sputum is low in the control group. It shows that the red chicken vine vine acid alcohol extract can not only reduce the fasting Blood sugar, and can improve glucose tolerance. & Table 5. Effect of five-week red chicken vine vine acidic alcohol extract on body weight and serum physiological indexes of high fat-induced mice serum three serum total serum 姨 final acid glycerol cholesterol island Non-esterified leptin weight ester (Serum Serum fatty acid (Leptin (Serum (NEFA)) TC) insulin TG) '--- (mol/ml) (mmol/l) (ng/ml) (mmol/|) Nq/ml) LF(n = 26.2 + 0.99 soil 2.62 soil 0·39± 1.27 soil 467 soil 1.2C 0.23b 0_43c 0.11b 0.16a 1557c 19 200906431 HF(n = ---- 37·0± 1.38± 6~7) 1.8a 0_18a HF + N 〇.11 (η 35.1± 1 ·14± =6 ～7) 3.2b 0_32b 4·68± _0.21a 3_69± 1.08b 1 ·63± 0.45a 1.28± 0.25a 〇_599± 0.2a 〇·462± 0.2b 5911± 16233 4204 soil 2209b The values shown are the mean ± standard deviation value (SD), 'Main Statistical analysis, with the P value (p_value) &lt; 〇〇 5 as the benchmark superscript a, b, c represents a significant difference. Table 5 shows that 15 〇〇 mg / kg b kiss red chicken vine extract It can significantly reduce the gallbladder, triglyceride, hypertonic acid secretion and blood leptin depth caused by high-fat diet. - The present invention is made by a simple preparation method, Moreover, it is not necessary to use a special agent or a complicated extraction step to achieve the method of the invention. The red vine extract extracted by the method of the invention can prove the pot and has the functions of regulating gold sugar and blood fat, and inhibiting sugar. The activity of the enzyme and the coenzyme a catalyzed enzyme can also promote the activity of sulfhydryl _ A oxidase, so the improvement of the metabolic syndrome can be said to be a great boon for patients with metabolic syndrome. Is a preferred embodiment of the invention, not the invention: </ RTI> <RTIgt; </ RTI> <RTIgt; </ RTI> <RTIgt; </ RTI> <RTIgt; </ RTI> <RTIgt; </ RTI> <RTIgt; The technical features of the present invention are still within the scope of the technical features of the present invention. BRIEF DESCRIPTION OF THE DRAWINGS The first drawing is a flow chart of the preparation of the present invention. The second graph is a linear graph of the activity of the red chicken vine vine acidic alcohol extract of the present invention for inhibiting the activity of the acetyl-CoA carboxylase.

The second (A) graph is a histogram of the activity of oxynase in different doses of phenyl butyl acrylate. A

[Main component symbol description] Red chicken vine vine alcohol extract (a) Provide red chicken vine

(e) Preparation of a pharmaceutical composition (d) Acquisition of a red chicken vine extract (f) Preparation of a food composition 21

Claims (1)

200906431 X. Patent application scope: 1 A red chicken vine extract, which is prepared by a method comprising the steps of: providing fresh or dried red chicken stalks and roots; extracting the red with an alcohol solvent The stems and roots of the chicken vines are obtained for obtaining an alcohol solvent extract; 'concentrating and drying the alcohol solvent extract; and obtaining the extract of the red chicken vine. 2 · The red vine extract of the red chicken as described in the scope of the patent application, wherein the alcohol solvent used for extracting the stem portion and the root of the red chicken screen can be mixed with water to become i~ degree at 25~1〇〇 Vvt% ethanol. 3. The red chicken vine extract according to claim 1, wherein the alcohol solvent used for extracting the stem portion and the root of the red chicken is mixed with water to a concentration of 70 to 1 〇〇wt〇/ 0 ethanol. [4] The extract of the red chicken vine as described in claim 2 or 3, wherein the ethanol further contains hydrochloric acid to become an acidic alcohol. 5 · The red chicken screen and vine extract according to item 4 of the patent scope, wherein the extract of the red chicken vine and the root system uses acidic alcohol, and the volume of the original vine and the acid alcohol is about 1:5. The ratio range of 1:2Q is performed at room temperature. A red chicken raw vine extract, the preparation step comprising: providing fresh or dried red chicken (four) series or partial · solvent extraction of the red chicken raw vine plant or part to obtain solvent extraction 22 200906431 concentrated drying the solvent extract And obtaining a red chicken vine extract, wherein the red pheasant extract is used to improve metabolic syndrome. 7. The extract of the red pheasant vine as described in claim 6 of the patent application, wherein the root portion and the stem portion of the red pheasant are provided. 8. The extract of the red pheasant vine as described in claim 7, wherein the group of the solvent of the red pheasant or the partial solvent is extracted: water, an alcohol, an aqueous alcohol solution, and an acetic acid Substance 9. The red chicken vine extract according to claim 8, wherein the solvent is ethanol which can be mixed with water to a concentration of 25 to 1 GGwt%. 1〇· "Please select the red chicken vine extract as described in item 8 of the patent scope", wherein the solvent is a mixture of water and water to a concentration of q7q~(10)(4), such as the third item of the patent scope, wherein the ethanol further contains hydrochloric acid. It becomes an acidic alcohol. The extract of the red vines and the roots of the red vines as described in the eleventh article of the patent scope (4) alcohol, at the room temperature, and the weight of the vines and the acid alcohol are carried out at room temperature. The ratio of the main 1·20 ranges from 1 to 3. According to the patent application range 6 to the extract of the red hen, the sugar syrup syrup, the inhibition (4) the syndrome group includes inhibition of the intestinal coenzyme A oxidase, Promoting liver sputum base, ie, blood sugar concentration and regulating blood concentration. A pharmaceutical composition for improving metabolic syndrome, which is a 23 200906431 agent, which includes red chicken screen, rattan extract and pharmaceutically acceptable carrier. Or the preparation step in the shaping comprises: providing fresh or dried red pheasant plants or parts; extracting the red pheasant plant or the part by solvent to obtain solvent extraction, concentrating and drying the solvent extraction Obtaining a red chicken vine extract; and adding the red chicken vine extract to a pharmaceutically acceptable carrier or excipient' to obtain a medical composition D 1 5 as described in claim i. The pharmaceutical composition for improving metabolic syndrome, wherein the root portion and the stem of the red pheasant are provided. 1 6 · The pharmaceutical composition for improving metabolic syndrome according to claim 15 of the patent scope of Shen Qing 'In the preparation step of the extract of the red pheasant, the solvent is selected from the group consisting of water, alcohols, aqueous alcohol solutions and ethyl acetate. 1 7 · as claimed in the patent scope The pharmaceutical composition for improving metabolic syndrome according to the above-mentioned item 6, wherein the solvent is ethanol which can be mixed with water to a concentration of 25 to 1% by weight, and is as described in claim 16 of the patent application. The pharmaceutical composition for improving metabolic syndrome, wherein the solvent is ethanol capable of mixing with water to a concentration of 7 〇 to 100% by weight. 19· for improving metabolic syndrome as described in claim 16 of the patent application. a pharmaceutical composition wherein the ethanol Further, hydrochloric acid is added to be acidic 24 200906431 Alcohol. Symptoms ΙΓ μ Please refer to the pharmaceutical composition for improving metabolic syndrome described in Item 19 of the patent range, using acidic alcohol in the pot, and the roots and roots of the red material H chicken are attached to (1). It is carried out at room temperature in the range of the ratio of the artificial vines to the original vines and the acid alcohol volume of about 1:5 。. The use of the metabolic syndrome according to any one of claims 14 to 20 The pharmaceutical composition, wherein the improved metabolic disease yeast system includes inhibition of intestinal glycolytic enzymes, inhibition of liver spleen supplementation, "into the liver, sputum, enzyme A oxidative tone, A, 上··················································· . Emulsifying enzymes, ie, blood sugar concentration and ^, 2 2 - a food composition suitable for patients with metabolic syndrome, beans including red chicken vine extract, 1 system + boring include: supply or dry &lt;, a chicken or vine plant or a part; a solvent; extracting the red vine or vine from a solvent to obtain a solvent extracting and concentrating and drying the solvent extract; obtaining an extract of red carp, and extracting the vine; The composition is used as an improved generation dr: the metabolic syndrome and the stem portion described in item 22 of the patent scope: K ° °, and "Do not use the red chicken vine root part root group name provided 24. The food composition suitable for the human being as described in claim 23, wherein in the process of preparing the red vine extract, in the second step of 25, 2009, 064, 31, the solvent is selected from the group consisting of water, Alcohols, alcoholic aqueous solutions, and ethyl acetate. 2 5 · A food composition suitable for patients with metabolic syndrome as described in claim 24, wherein the solvent is capable of mixing with water to a concentration of 25 to 1 〇〇 Wt% ethanol. 2 6) A food composition suitable for a metabolic syndrome patient as described in claim 24, wherein the solvent is ethanol capable of mixing with water to a concentration of 70 to 100% by weight. 2 7 · Patent Application No. 2 The composition of the above-mentioned H composition suitable for patients with metabolic syndrome, I, and the acid containing acid to become acidic alcohol 0 2 8 . The food combination suitable for patients with metabolic syndrome as described in claim 27 of the patent scope Tb St , ^ 物 , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , In the range of ratios, it is carried out at room temperature. 2 9 · The application of the patent scope 2 之 乐 乐 乐 ～ ～ 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 The substance, which improves the metabolism of the 伎 包括 包括 包括 包括 包括 包括 包括 包括 包括 包括 包括 包括 抑制 抑制 抑制 抑制 抑制 抑制 抑制 抑制 抑制 抑制 抑制 抑制 抑制 抑制 抑制 抑制 抑制 抑制 抑制 抑制 抑制 抑制 抑制 抑制 抑制 抑制 抑制 抑制 抑制 抑制 抑制 抑制 抑制 抑制 抑制 抑制 抑制 抑制 抑制26