TWI331921B - - Google Patents

Description

1331921 IX. Description of the invention: [Technical field to which the invention pertains] The present invention relates to a red pheasant extract, in particular, a substance capable of inhibiting intestinal glycolytic enzymes and acetylated coenzyme A carboxylate in the liver (acetyh CoA) Carboxylase, ACC), and promotes the activity of AcyhCoA oxidase (ACO) in the liver to improve the metabolic syndrome of red pheasant. [Prior Art] U.S. Patent No. 5,531,991 discloses a "composition and method for treating hyperglycemia using extracts of P〇丨ygonum muitin〇rum", which is a type of Polygonum multiflorum. The extracted extract of ammonium hydroxide is divided into three components (fract丨on) by glucopolymer G_25 (sephadex G-25), and the extract of Heshou has similar effects of increasing insulin activity and regulating blood sugar. The function. In the U.S. Patent No. 6,200,569, a "composition and method for increasing insulin activity" is disclosed, which is a method of using bacteria or silver in Heshou Island (Hou (10), m (four), viscera _) and agaric (4) (10) coffee. Ye Gui (C/Qing Qiong (7) position·re/) composed of compound water extract*, the compound water extract has the effect of increasing insulin activity, the effect of Zhuang, and the regulation of blood sugar. The use of Polygonum multiflorum (Ρ·_ domain tearing _ as a raw material, and to increase the blood sugar by the mechanism of 増 姨 素 素 , , , , , , , , , , , , , 血糖 血糖 血糖 血糖 血糖 血糖 血糖 血糖 血糖 血糖 血糖 血糖 血糖 血糖 血糖 血糖 血糖 血糖 血糖 血糖 血糖 血糖 血糖82951 9A1 Patent "A pharmaceutical composition for treating and preventing or preventing hyperlipidemia and a method for producing and using the same" which discloses a extract from Hosho Mountain, Tandan and Shanqi a compound composition consisting of - a proportional composition having a function of regulating blood lipids, and @, the result is only presented in the human body test: the result ' does not reveal a possible mechanism of action. / However, the above-mentioned European patent The use of Polygonum multiflorum as a raw material, and must be combined with other extracts to form a combination of substances to regulate blood lipids 'but it does not reveal the effect of regulating ▲ sugar or improving metabolic syndrome. In summary, the existing decline The composition of blood sugar or blood fat lowering is based on the general traditional Polygonum multiflorum (po/yfif〇(7)(7)叻//7〇π straight to the mainland of China, and the medicinal part of the traditional Polygonum multiflorum lies in it: large = root 0 - again There is only a single-function music or food that lowers blood fat or lowers blood sugar. There is no drug or food for metabolic syndrome. However, the occurrence of metabolic syndrome is more and more common, so this study is to screen the intestines. The phytoplasma that absorbs the sugar, inhibits the synthesis of fatty acids, and promotes the oxidation of fatty acids, and the related products that solve the metabolic syndrome. [Invention] The present inventors are also concerned that the Polygonum multiflorum currently used is a traditional Polygonum multiflorum and only has a single - the function of lowering blood sugar or lowering blood fat without any research on the treatment of metabolic syndrome, so after Time study 1331921 = and after continuous trials, the red chicken vine extract with improved metabolic syndrome has been invented. · The purpose of the second invention is to provide a single use of red chicken vine (also known as Taiwan Polygonum) The extract is such that it inhibits intestinal glycolytic enzymes and the activity of acety-coagulase (acety丨_c〇A ca "b〇xy丨ase acc) in the liver ^^^^(acyl-C〇) The activity of A oxidase (ACO) for improving the metabolic syndrome of red (tetra) vine extract. To achieve the above object, the present invention relates to a red chicken screen and vine extract for improving metabolic syndrome, which includes the following Prepared by the method: providing a fresh or dried red vine plant or part; extracting the red pheasant plant or part by solvent to obtain a solvent extract; concentrating and drying the solvent extract; and obtaining the red chicken vine extract Things. Preferably, the red pheasant is provided with a stem and a root. Preferably, the solvent for extracting the local or partial plant of the red pheasant can be water, an alcohol, an aqueous alcohol solution and ethyl acetate, wherein the water can still be used to carry out the red pheasant plant or local part at an elevated m·degree. The extraction method can directly put the water into the pot of the traditional Chinese medicine for cooking, and directly heat the red chicken or the vine plant or the whole portion; more preferably, the concentration of the solvent is 25~1〇〇wt〇/ . The optimum ethanol concentration of the pure ethanol or aqueous ethanol solution is 70 to 100% by weight; most preferably, the alcohol solvent is an acidic alcohol containing hydrochloric acid. Preferably, the extract of the red pheasant vine stem and the root system using acidic alcohol, and the ratio of the weight of the red pheasant and the acid alcohol is about 1:5 to 彳:2 ,, at room temperature. . The present invention relates to a pharmaceutical combination 1331921 for improving metabolic syndrome, which comprises red chicken raw vine extract and medically, pharmaceutically acceptable carrier or shaping, Qi! The preparation step of the red chicken raw vine extract comprises: providing fresh or red chicken raw vine plants or parts; extracting the red chicken raw vine plants or parts by solvent to obtain a solvent extract; concentrating and drying the solvent extract; obtaining red chicken An original rattan extract; and the red chicken raw vine extract is added with a pharmaceutically acceptable carrier or excipient to prepare a pharmaceutical composition for use in improving metabolic syndrome. Preferably, φ is provided with a stem portion and a root portion of the red vine. Preferably, the solvent for extracting the red vine or the partial vine is water, an alcohol, an aqueous alcohol solution and ethyl acetate, wherein the water can still be extracted or partially extracted by the red vine. The system can put the water into the _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ Good ethanol concentration is 7〇~(10) gift. Most preferably, the alcohol solvent is an acidic alcohol containing hydrochloric acid. _ * Yes, extract the red stalk from the original stem and root (4) using acid alcohol, in the red chicken # vine weight and acid alcohol volume is about [5 to 1: deduction range, at room temperature. The present month is also related to a food group suitable for patients with metabolic syndrome. The second group includes red chicken raw vine extract, and the preparation steps include: providing fresh or dried red chicken 屎藤植', p> The M cold agent takes the red chicken I part to obtain a solvent extract; the solvent extract is concentrated and dried, and the red chicken raw vine extract is obtained; and the food composition is prepared to improve the metabolic syndrome. " 1331921 Preferably, the stems and roots of the red broiler vines are provided. Preferably, the extraction of the red '厍 虽 虽 虽 妹 妹 妹 或 或 或 或 或 或 或 或 或 或 或 或 或 或 或 或 或 或 或 或 或 或 或 或 或 或 或 或 或 或 或 或 或 或 或 或 或 或 或 或 或 或Partial extraction, which can be used to put water into the pot of the traditional Chinese medicine, and directly cook the red chicken (four) plants or parts, preferably the solvent is pure ethanol with a concentration of 25~wt%. Or an aqueous alcohol solution, the optimum ethanol concentration is 7〇~i〇〇wt%; optimally, the alcohol solvent is an acidic alcohol containing hydrochloric acid. Preferably, the stems and roots of the red pheasant are extracted using acid alcohol, and are carried out at room temperature within a ratio of the weight of the red pheasant and the acid alcohol volume of about 1:5 to 1:20. Since the steps of the present invention are simple, and the effective energy of the red pheasant extract can be extracted without special solvent, the cost is low, and the glycolytic enzyme in the intestine and the acetaminophen coenzyme A carboxylate in the liver (Acety丨) -c〇a

After the experiment of Ca "b〇Xylase, ACC", it was confirmed that it had an inhibitory effect and promoted the activity of the 醯Kymase hexaoxidase (Acy|_c〇A 〇xjdase, ACO> in the liver. Therefore, the present invention only needs to utilize The single-red chicken vine extract can simultaneously achieve the function of regulating blood lipids and regulating blood sugar, and has the effect of improving metabolic syndrome. [Embodiment] Referring to the first figure, the present invention relates to one for An improved red locust extract of the metabolic syndrome, which is prepared by the following method, which comprises providing fresh or dried red pheasant plants or parts (a); extracting the red pheasant plant or part by solvent Obtaining a solvent extract (b); concentrating dry 921 to know the bath extract (C); and obtaining a food group containing the red chicken vine extract (d) Or the package (4) is to use the solvent to draw the fresh or dried red vine plant, and use the solvent to dissolve the active ingredient in the solvent contained in the red chicken, and further benefit ~ Use this soluble The active ingredient in the solvent, the enzyme in the intestinal glycolytic enzyme and the liver M + machinery, which is found to regulate blood sugar and blood lipid regulation key enzyme activity. In a specific embodiment of the present invention, it can be found that the use of the soluble The active ingredient in the solvent has the activity of inhibiting intestinal glycolytic enzyme, liver coenzyme A-lowering enzyme, and can promote the activity of hepatocyte 醯Kease A oxidase. Therefore, the red chicken vine extract obtained by the invention The pharmaceutical composition or the related health food for treating metabolic syndrome can be prepared by further mixing with a pharmaceutically acceptable carrier or excipient. The "Red Chicken and Rattan", also referred to as Taiwan Polygonum multiflorum (also referred to as Taiwanese Polygonum) Tajwan Tuber Fleeceflower) 'The origin of Taiwan is Taiwan, which is an intrinsic species of Taiwan. It does not have obvious roots. It is a variant of traditional Polygonum multiflorum. It is also a kind of traditional Chinese medicine. It is common in the middle and low altitude mountains of Taiwan. It grows on the roadside and Lin Yuan, but no one has used the roots of Polygonum multiflorum in Taiwan to replace the traditional Polygonum multiflorum. The red pheasant belongs to the genus (7)) and the genus Po/ygonaceae. ’ The scientific name can be po/ygo(10)m mw/i/"〇a7m

Thunb· Ex murray var. hypoleucum (Ohwi) Liu, Ying & Lai, Pleuropterus hypoleucus multiflorum, Polygonum hypo/euctym Ohwi and Po/ygonum mty/i/77〇rum Thunb· var· 1331921 (〇hWi) Liu,Ying & Lai, the root and the vine (stem) part can cough, hurricane, phlegm 'treatment feeling c cough rheumatism, etc.; and the leaves; to treat colds, external wounds and so on. The term "metabolic syndrome" (metb〇||c syndr〇r^) means 姨 ( 阻抗 impedance syndrome (ins_(四) 咖...., i is a type 2 diabetes patient with obesity and high blood Houses, words, gray days and other symptoms. And the membrane island Yin Yang p 叩 islet resistance (丨nsu丨m resistanc = Γ: 共同 common pathogenic turnaround) can be suppressed by: auxiliaries... _ Kiev enzymes, control of blood sugar, blood lipid concentration and improved. Chicken thickness == refers to the "red chicken raw vine extract" means the use of solvents for red vines into the plant = extract 'to obtain red chicken vines soluble in solvents The solvent may be water, an alcohol such as ethanol, an aqueous alcohol solution, and a subsequence =, and the ethanol is preferably an acidic alcohol. In the present invention, the red chicken may be a fresh or dried whole plant. Preferably, the system is: -=:: Γ (4) vine roots, etc.; in the present hair drying system, decompression concentration method, cold drying method. ' : Concentrated dry matter known to those who are generally known as "medically acceptable load" Agent, if the pharmaceutical combination is in the first two positions, the carrier can protect the pharmaceutical conjugate. It does not reach the decomposition of the drug release system, and it loses its effectiveness, and, or can release the enemy and the effect. The uniformity of the medicine of the present invention, the stable and the durable excipients are Adding such as M " 疋 and reducing the irritation of the drug 'bad odor, etc., such as sugar alcohol, starch or 丄 丄 丄 。 。 。 。 。 。 。 。 。 。 赋 糖 赋 赋 赋 赋 本 本 本 本 本 本 本The vinegar group's #%, which can be made into a therapeutic composition that improves the recovery of metabolic syndrome, is given orally in different forms, including but not limited to, θ people / 4 years old alone in the form of red chicken Oral vine extract or = acceptable carrier, the form of oral administration may be selected from the group consisting of a tablet, a powder, a suspension, a sputum, a sputum capsule, a granule, a tablet, a drug, a liquid, an alcohol, and a medicated drug. In the group consisting of syrups, 'The following examples are for the purpose of specifically explaining the technical means of the present invention, and the activity of the red hen extract of the present invention is reacted with a specific enzyme to carry out an activity analysis. Preparation of extracts Enemy enemies: After grinding the red chicken raw vines with a grinder, extract with acidic alcohol (addition of U hydrochloric acid (HC丨)), the ratio of the weight of red hens to the acid alcohol = about 1: 8 (W / V = 1/8), place it at room temperature for τ or j; 2 "Sf is oscillated at 1000 rpm. After 72 hours, use gauze for preliminary filtration. Centrifuge at 6000 rpm for 15 minutes, concentrate on & μ a and concentrate to dry to an acidic alcohol extract with an extraction rate of about 6.9%. Re-dissolve the extract to a concentration of 5 〇 mg/ m丨.

12 1331921 Hot water benzene loser Put the red chicken vine into the Chinese medicine pot and cook for about two hours. The ratio of the weight of the red chicken vine to the water is about 1:20 (W/V = 1 /20) ' Filtered with gauze, the filtrate was lyophilized to obtain a hot water extract with an extraction rate of about 9.2%. The extract was reconstituted to a concentration of 50 mg/ml as the acidic alcohol extract. The following experiment. Example 2. Inhibition of α-glucosidase by P. sinensis extracts p-nitrophenyl a -D-glucoside (pNP-G) Analytical method for analysis of inhibition of glucosidase activity. The methods include: 1. Firstly, 1 U/ml of glucoside: glucosidase (EC 3.2.1.20, G5003, configured with phosphate buffer solution (100 mM, pH 7.0). Sigma-Aldrich Co., USA) solution and 5 x 10_3M p-nitrobenzene·α _D_. P-nitrophenyl a -D-glucopyranoside.pNP-G, Sigma-Aldrich

Co·, USA) The solution was taken up. 2. Then add 4 μL of acid buffer solution (0.4 M, pH 7.0) and 1 μL ("丨) of α-glucoside in each well of a 96-well plate. The enzyme solution, as well as different dilutions of the red chicken vine extract 5 〇 microliters (/y|), then add deionized water to 170 microliters (Chuan). 3. Allow the incubator to warm up for 5 minutes at 37 ° C, then add 3 μl of microliter (7) 丨) preheated substrate, mix thoroughly and shake, and apply at 37 13 °c. minute. 4: The photometric analyzer with a wavelength of 4〇5 nm (nm) bribes... k C〇·, USA> The absorbance value before and after the record, and the suppression of 50% by ic50. The concentration required for glucosidase activity. See Table 1, which shows the results obtained with acidic alcohol extract and hot water extract: Table 1. Acidic alcohol extract #1 Acidic alcohol extract #2 Hot water extract IC5. (Extract #Q/ml) 5·5±0·5 6.5±0.7 17.1±0.4 Fine It can be seen from Table 1 that the acidic alcohol and hot water extracts of the red pheasant are inhibited by the activity of the heart glucosidase. The effect of the == alcohol extract is better. Example 3. Inhibition of different glycolytic enzyme activities by Red Chicken and Rattan extracts μ Different sugars were administered using a crude extract of rat intestinal enzyme (rat jntestina, curry powder, Sigma Chemical Co., St. Louis, source). Acceptance (maltose, recommended sugar and house powder) to test the inhibitory effect of acid alcohol red material on the activity of *glycolysis enzyme. Please refer to Table 2, which shows the use of acid alcohol red chicken raw vine extract 14 1331921 For the inhibition of different glycolytic enzyme activities, it also uses 丨c5〇 to indicate the inhibition of activity: Table 2. -----_ ICS0 (extract mg/ml) lysing octyl starch lysin sucrose Decomposing Acidic Alcohol Extract----- V Scary}S*T Jjs 0.6 —1 -^ 1.9 3.1 As shown in Table 2 above, the acidic alcohol extract for maltose is a maltase '; Both (^ _arny|ase) and sucrose saccharide (sucrase) have an inhibitory effect, and the inhibitory strength is maltose lyase [starch sugar lyase] sucrose saccharolytic enzyme. Example 4. Red chicken vine Preparation of fat-soluble extracts After grinding by the attritor, the volume ratio of the weight of 'Red Chicken vine' and the 50% by weight aqueous solution of decyl alcohol extracted by the concentration of 50% by weight of methanol water is 1: i〇(w/v=i/i〇). After shaking for 24 hours at room temperature, it was filtered through a non-woven fabric, and the obtained filtrate was added with an equal volume of ethyl acetate (ethy丨acetate)/water, and the mixture was shaken and mixed, and then subjected to liquid-liquid extraction to obtain an ethyl acetate layer of the upper layer, followed by addition of acetic acid. Ethyl acetate equivalent volume of 5wt% sodium bicarbonate (s〇dium

Bicarbonate, NaHC〇3) was shaken and mixed, and the sodium bicarbonate layer was removed to wash away the free fatty acid, followed by concentration and drying of the ethyl acetate layer to obtain the desired fat-soluble extract, and the extraction ratio was about 91%.

15 1331921 The fat-soluble extract is reconstituted with Dimethyl Sulfoxide (DMSO) to a concentration of i (jmg/ml. Example 5. Acidic alcohol extract and fat-soluble extract of Red Chicken Activity Analysis of Acety CoA Carboxylase (ACC) is a key enzyme in the synthesis of fatty acids. The product is a malonium-based enzyme A (malony). It is a receptor for fatty acid synthase (FAS) and is an inhibitor of carnitin palmitoyl CoA transferase (CPT). Therefore, inhibition of ACC not only inhibits endogenous lipid synthesis, but also accelerates the grain line. Oxidation of fatty acids in the body. ACC was purified from rat liver and tested by the method of Tanabe et al. (Tanabe T., Nakanishi, S., Hashimoto T., Ogiwara, H., Nikawa J., Numa S. 1981. Acetyl-CoA carboxylase from rat liver, Meth. Enzymol. 71(pt C): 5- 16). The results obtained are shown in Table 3: Table 3. Percentage of inhibition of ACC activity (%) Acidic alcohol extract (0.05ma/ Mjj_ 61.27 + 5.4 踔 dissolve, (〇 〇5mg/ml) 45.99 + 11.9 16 I3W丄 As can be seen from the above Table 3, the red chicken jin _ hemp · · chicken 屎 之 acid alcohol and fat-soluble extract sound has a good ACC inhibition sting activity. Then take acid alcohol extraction The quantity is measured by 丨〇5〇. Please look at the first-R _ heart and the younger one. It is the linear change of the concentration of the acid alcohol in the red vine and the inhibition effect. Red 1 ^ Teng (4) _ extract reached (four) 50% of the ACC activity required concentration (IC50) was 0. Q4 mg / ml. Example 6. Red chicken vine vine acidic alcohol extract promote 醯 Kiev enzyme a oxidase ( Activity analysis of Acyl-CoA oxidase (ACO) 醯Kyivase A oxidase (AC0) is the key enzyme for the oxidation of fatty acids, and is also a typical downstream gene of peroxisome proliferator-activated receptor σ (ppARcy). As an indicator of the oxidative metabolic activity of fatty acids. This analysis is to inoculate H4IIEC3 hepatocytes in appropriate density in a 12-well plate, and replace them with pentylene ketone (C) which is known to promote AC 〇 performance. |0fibrate) or 彳〇〇 升/ml (/y|/m丨) of red chicken The original vine acidic alcohol extract was cultured for a suitable period of time, and the cells were collected after aspirating the culture solution. The AC〇 activity test was conducted by scholars such as smal丨 (Small GM. Burdett K. ConNock MJ. 1985. A sensitive spectrophotometric assay for peroxisomal acyl-CoA oxidase. B/oc/7em/ca/ Jot; factory na/· Method in 227: 205-210.). Please refer to the third figure, where the third (A) diagram shows the use of different concentrations (100//M, 500//M ' ΙΟΟΟρΜ) of gas butyl acrylate at different times (48 and 72 hours) The degree of ACO activity can be promoted; and the third (Β) Figure 17 1331921 • indicates the increase in ACO activity after 48 hours of treatment with 1 〇〇W/ml of red chicken vine vine acidic alcohol extract. Example 7. Effect of red chicken vine vine acidic alcohol extract on high fat-induced metabolic syndrome C57BL/6J mice C57BL/6J mice were first fed with high fat diet containing 30% (g/g) cream for eight weeks. To cause symptoms of metabolic syndrome such as obesity, hyperlipidemia and hyperinsulinemia. The mice with symptoms of metabolic syndrome were randomly divided into two groups, and one group was given a high-fat diet supplemented with 3% (g/g) of red chicken vine vine acidic alcohol extract (equivalent to 1 kg per kg of body weight). 5 mg of red chicken vine alcoholic alcohol extract (1500 mg/kg body weight) (HF + No.1l), another group maintained high fat diet as control group (HF). There is also a normal feed control group * (LF). The oral glucose tolerance test (0GTT) was performed after five weeks of feeding, and the results are shown in Table 4. Subsequent sacrifice of the mouse's final body weight and blood biochemical analysis results are shown in Table 5. Table 4. Changes in blood glucose in mice of each group at the 5th week of oral glucose tolerance test

Blood glucose (mmol/L) AUC^Omin Omin 30min 60min 90min 1 20min (mmol*min/L) LF (n = 6.1±1. 10.37 soil 9.97±1·8·75±1. 8.56±1 656.3±93.3 a 14 32a 1.45 a 25 a 52 a 55 a HF(n = 8·76± 12·27± 12·16± 12·25± 13.74±961.2±76.3C

18 丄 6~7) 2.78 b HF + N 0.1 1 (η = 6~7) 7.6±1. 27a

· The fruit is measured by the mean value (SD). The superscripts with different letters represent significant differences, P<〇 〇5. — ·'"Not, '······················································································· The 60- and 90-minute sugars of the oral glucose tolerance test and the area under the 2-hour blood glucose curve (a "ea unde" called a one, auc) were significantly lower than those of the control group. The extract can not only reduce fasting blood sugar, but also improve glucose tolerance. Table 5. Effect of five-week red chicken vine vine acidic alcohol extract on body weight and serum physiological indexes of high fat yang-qi C57BL/6J mice Final weight serum triglyceride (Serum TG) serum total cholesterol (Serum TC) serum insulin Serum insulin non-esterified fatty acid (NEFA) alizarin (Leptin) ώλΙ (mol/ml) (mmol/l) fng/ml) ( Mmmol/l) (ng/ml) LF (n = 26.2 + 0.99 soil 2.62 soil 0.39 ± 1.27 ± 467 soil 14) 1.2C 0.23b 0.43° 0.11b 0.16a 1557° 1331921

HF (n = 37 · 〇 ± 1.38 soil 4.68 soil 0.599 soil 1.63 ± 5911 ± 1.8a 0.18a 0'21a 0.2a 0.45a 1623a hf + n 〇 · 11 (η 35.1 ± 1.14 soil 3.69 ± 0.462 ± 1.28 ± 4204 ± =6~7, 3.2b 0.32b 1,08b 0.2b 0_25a 2209b The values shown are the mean ± standard deviation value (SD). Statistical analysis 'P value (p_va|ue) < 〇〇 5 as a reference The superscripts a, b, and c represent significant differences. Table 5 shows that 'giving 1500 mg/kg body weight of red chicken vine vine alcoholic alcohol extract can significantly reduce high cholesterol, high triglyceride caused by high fat diet Ester, high insulin secretion and blood sputum (丨eptj concentration. The present invention is prepared by a simple preparation method, and can be achieved without using a special agent or a complicated extraction step, and is carried out by the method of the present invention. The extracted red chicken vine extract has been tested to prove that it has

It regulates the functions of blood sugar and blood lipids, and inhibits the activity of glycolytic enzymes and SI-CoA-lowering enzymes. It also promotes the activity of oxidizing enzymes of Kiev enzyme, thus improving metabolic syndrome. For patients with metabolic syndrome, Great gospel. The above description of the present invention is merely a preferred embodiment of the present invention, and is: a limitation on the formula, any technique having the general knowledge in the art, and not departing from the technical features of the present invention. Equivalent to the local change or modification of the content

20

Within the scope of the feature. The content of the levy still belongs to the technology of the present invention. [Simplified description of the drawings] The first drawing is a flow chart of the preparation of the present invention. The second graph is a linear plot of the activity of the red cod vinegar acid coenzyme A carboxylase of the present invention. Alcohol Extract Inhibits Acetylene The second (A) diagram is a bar graph of the activity of different enzymes of chlorpheniramine to promote the activity of 醯K. The second (B) graph is a histogram for promoting the activity of the lytic enzyme A oxidase by using the 1 〇〇/yg/m丨 of the red cockroach vine alcohol extract of the present invention. [Explanation of main component symbols] (a) Providing red chicken vine (b) extracting red chicken vine to obtain extract (c) concentrating dry extract (d) obtaining red chicken vine extract (e) preparing pharmaceutical composition (f) preparing a food composition 21 < S >

Claims (1)

1331921 Announcement ^-- X. Application Patent Range: Amendment 1 1 A red chicken vine extract, the preparation steps include: providing fresh or dried red chicken vine plants or parts; Extracting or partially extracting the red pheasant plant to obtain a solvent extract; concentrating and drying the solvent extract; and obtaining an extract of the red carp, the vine extract, for improving metabolic syndrome. 2. The extract of the red pheasant vine as described in claim 1, wherein the root portion and the stem portion of the red pheasant are provided. 3. The extract of the red pheasant vine as described in claim 2, wherein the solvent extracted from the § 亥 红 屎 植 或 或 或 或 或 或 或 或 或 或 或 或 水 水 § § § § § § § § § § § § § § § § An aqueous solution of an alcohol and a beta acetate of the acetic acid according to claim 3, wherein the solvent is an ethanol which can be mixed with water to a concentration of 25 to 1% by weight. 5. The extract of Dioscorea opposita Thunb. according to claim 3, wherein the solvent is ethanol which can be mixed with water to a concentration of 7〇I 〇〇wt〇/0. [6] The red chicken vine extract according to claim 3, wherein the ethanol further contains hydrochloric acid to become an acidic alcohol. 7. The red chicken vine extract according to claim 6, wherein the raw vine stem and the root of the red hen are extracted with acidic alcohol, and the weight of the red pheasant and the acid alcohol is about 1:5. In the range of the ratio of 20: 20, the red chicken 22 1331921, the vine extract, as described in any one of claims 1 to 7, wherein the metabolic syndrome, the enzymes of the group are improved. Inhibition of Hepatic Acetyl Coenzyme A Carboxylase ' Intestinal Glycooxidase, Regulating Blood Glucose Concentration, and Regulating ▲ 旨 旨 肝脏 肝脏 肝脏 肝脏 肝脏 9. 9. 9. 9. 9. 9. 9. 9. 9. 9. 9. 9. 9. 9. 9. 9. 9. 9. 9. 9. 9. 9. 9. 9. 9. 9. 9. And the pharmaceutically acceptable preparation step comprises: a scorpioning agent, wherein the fresh or dried red pheasant plant or the sorghum is provided; the red squid plant or part is extracted by solvent: obtaining the solvent The solvent extract is concentrated and dried by extraction; the extract of the red pheasant is obtained; and the extract of the red pheasant is added to the medicinal agent to obtain a pharmaceutical composition. Further, the carrier or the trait of the medicinal composition of the medicinal composition of claim 9, wherein the medicinal vine has a partial stalk portion. [11] The pharmaceutical composition for improving metabolic syndrome according to claim 10, wherein in the preparation step of the red (four) vine extract, the solvent is selected from the group consisting of the following substances; : water, alcohols, alcoholic aqueous solutions and acetic acid. 2. The composition of (4) for improving metabolic syndrome according to the above-mentioned application of the patent scope, wherein the solvent is ethanol which can be mixed with water to form a concentration of 25 to 100% by weight. X 1 3 . As claimed in the patent scope! The work described in the work for improving metabolism 23 «31921 wherein the solvent is a pharmaceutical composition capable of mixing with water to a concentration in the syndrome, ~100% by weight of ethanol.丄4 · For example, the medicinal composition for improving metabolic syndrome, as described in item 1 of item 1, a 哕Γ醢 4 ^ 代潘洒, '" ethanol and then hydrochloric acid becomes acidic 15 ·Please refer to the pharmaceutical composition for symptom group described in item 14 of the patent scope, in which acidic alcohol is used, and in the red chicken = chicken raw stem and root system, the vine is reset and the acid alcohol volume is about 1 : Within the ratio range of 5 to 1.20, it is carried out at room temperature. 1 6 . For example, please refer to the second part of the patent scope 9 &1 5 - the pharmaceutical composition of the metabolic syndrome, "the improved metabolic syndrome system includes inhibition of the sugar disintegration and inhibition of liver B Tanning A slows, promotes the liver to brew Kiev enzyme A oxidase, regulates the blood sugar concentration to lang ash fat concentration. 17·-帛 Food composition suitable for patients with metabolic syndrome... Including the red chicken raw vine extract, the preparation step comprises: providing fresh or dry red pheasant plant or part; ' l. extracting the red hen original plant or part by solvent to obtain solvent ftm " concentrating and drying the solvent Extract; obtaining red chicken vine extract; and using the red chicken vine extract as a food additive for the Xie syndrome. A food composition suitable for a patient with metabolic syndrome 24 1331921 as described in claim 17 of the patent application, in which the stem is stalked. /, "The root part of the roots of the red hens of the red hens" is provided in the food composition of the group of patients as described in Item No. 18 of the patent range. In the red chicken Xie syndrome, the solvent is selected. Prepare an aqueous alcohol solution and ethyl acetate from the following materials. Group·Water, Alcohol, 20 · The food composition of the group of patients as described in claim 19 of the patent application, in the pot, the 'cross surface π Xie syndrome m mixture can be mixed with water 25~1 〇〇wt% Ethanol. The food composition of the group of patients as described in claim 19 of the patent application, in the middle of the sputum, the six-over-symptoms, wherein the mixture is 70-100% by weight of ethanol with water. The field-agronomy is 22. A food composition suitable for a group of patients as described in claim 19, wherein the ethanol is in/out of the essence. And 3 has salt...acid wine 23 ·If the food composition for the metabolic syndrome patients described in the 22nd item of the patent scope is extracted, the red chicken is used to extract the stem and the root system using acidic alcohol, and the weight of the red chicken is It is carried out at room temperature in a ratio ranging from about U to 1:20. 24. A food composition suitable for a metabolic syndrome patient according to any one of clauses 17 to 23, wherein the metabolic syndrome comprises inhibiting intestinal glycolytic enzyme, inhibiting liver A_enzyme, and promoting liver A system of A-oxidase, regulate blood sugar concentration and regulate blood lipid concentration. 25 1331921 Η—, 囷: 1331921 First picture 1201331921 Degree of inhibition of ACC activity (%) 100 80 60 40 20 0 0.001 0.01 0.1 1 10 Concentration of acid alcohol extract of red pheasant (mg/m 1) Second figure 1331921 (B) Multiple of AC0 activity increase ο 5 4 3 2 Multiples of increased ACO activity 4S hr. 72 hr. 3 2 48 hr time time third picture