CN101356968B - Red Chinese fevervine extract and medical composition thereof and food composition and preparation method thereof - Google Patents
Red Chinese fevervine extract and medical composition thereof and food composition and preparation method thereof Download PDFInfo
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Abstract
The invention relates to a red Chinese fevervine herb extract used for improving the function of metabolic syndrome and a medical compound as well as a food compound thereof; the invention discloses the red Chinese fevervine herb extract used for improving the function of metabolic syndrome; firstly, a medical compound which contains red Chinese fevervine herb extract and is used for improving the metabolic syndrome is yet to be disclosed and then a food compound which contains the red Chinese fevervine herb extract and is applicable to patients with metabolic syndrome; the invention is prepared by a simple preparation method, and is proved to have the function of adjusting blood sugar and blood fat, as well as restricting the activity of sucroclastic enzyme and carboxylated enzyme of acetyl coenzyme A; the invention can also enhance the activity of oxidizing enzyme of acyl coenzyme A; therefore, the invention can achieve the effect of improving the metabolic syndrome by only utilizing the single red Chinese fevervine herb extract.
Description
Technical field
The present invention is a kind of red Chinese fevervine extract; especially a kind of containing can be suppressed acetyl-coa carboxylase ferment (acetyl-CoA carboxylase in enteron aisle sugar solution ferment and the liver; ACC) activity; and acyl-CoA oxidation ferment (Acyl-CoA oxidase in the promotion liver; ACO) activity is to improve the red Chinese fevervine extract of metabolic syndrome.
Background technology
The U.S. the 5th, 531, No. 991 the patent case discloses a kind of " utilizing constituent and the method for the too high disease of the fleece-flower root (Polygonummultiflorum) extract treatment blood sugar ", it is a kind of with the extract of the fleece-flower root with the ammonium hydroxide extraction gained of 0.1N, be divided into 3 components (fraction) through Portugal coagel G-25 (SephadexG-25), and what first thing extract have the effect of similar increase insulin active and the function of regulating blood sugar.
And in the U.S. the 6th, 200, disclose a kind of " increasing constituent and the method for insulin active " in No. 569 patent cases, it is a kind of fleece-flower root (Polygonum multiflorum) and the bacterium of Agaricaceae (Agaricaceae) or compound water collection thing that Cinnamomum mairei Levl. (Cinnamomum mairei) is formed of utilizing, this compound water collection thing has the effect that increases insulin active, and the function of regulating blood sugar is arranged.
Yet, more than two United States Patent (USP)s disclose is to utilize the fleece-flower root (Polygonummultiflorum) as raw material, and regulate blood sugar with the mechanism that increases insulin active, therefore do not have the effect of regulating blood fat, more do not have the effect of improving metabolic syndrome.
In addition, a kind of in the patent case of EP1829519A1 " being used for the treatment of and/or preventing the method for medical composition and manufacturing and this medical composition of use of hyperlipemia ", it is the collection thing processed that discloses by the fleece-flower root, hawthorn, Dan Ginseng and mountain seven, the compound of forming by a certain percentage, this compound has the function of regulating blood fat, yet this result only is presented in the human trial result, does not disclose possible mechanism of action.
Yet above-mentioned European patent is to utilize the fleece-flower root as raw material, and other collection thing processed formed compound material of must arranging in pairs or groups has the effect of regulating blood fat, but it does not disclose the effect that has adjusting blood sugar or improve metabolic syndrome.
In sum, the composition of existing hypoglycemic or reducing blood lipid all is to utilize general traditional fleece-flower root (Polygonum multiflorum), and its original producton location is in the China's Mainland, and the medicinal part of traditional fleece-flower root is the piece root that it is loose.
Moreover, have only at present reducing blood lipid or hypoglycemic medicine or the food that waits simple function on the market, there is no at metabolic syndrome and develop medicine or food.Yet the situation that metabolic syndrome takes place is more and more general, thus this research namely be to screen have the absorption that suppresses enteron aisle sugar part, to suppress aliphatic acid synthetic and promote the vegetable raw material of fatty acid oxidation, to develop the Related product of solution metabolic syndrome.
Summary of the invention
Be all traditional fleece-flower root in order to overcome the fleece-flower root that utilizes at present; and the function of single hypoglycemic or reducing blood lipid only arranged; and do not have any about treating the defective of metabolic syndrome; the invention provides a kind of red Chinese fevervine extract of (having another name called the Taiwan fleece-flower root) that utilizes separately; make it suppress enteron aisle sugar and separate acetyl-coa carboxylase ferment (acetyl-CoA carboxylase in ferment and the liver; ACC) activity; and acyl-CoA oxidation ferment (acyl-CoA oxidase in the promotion liver; ACO) activity is to be used for improving the function of metabolic syndrome.
For achieving the above object, the present invention be relevant to a kind of be used to the red Chinese fevervine extract that improves metabolic syndrome, its be comprise with following method prepared: red Chinese fevervine plant or part that fresh or drying are provided; With this red Chinese fevervine plant of solvent extraction or local to obtain solvent extract; This solvent extract of concentrate drying; And obtain this red Chinese fevervine extract.
Preferably, the red Chinese fevervine part that provides is stem and root.
Preferably, the solvent that extracts this red Chinese fevervine plant or part can be water, alcohols, alcohol solution and ethyl acetate, wherein water still can carry out red Chinese fevervine plant or local extraction under the temperature that raises, it is the medicine kettle that water can be put into general infusion Chinese medicine, directly with red Chinese fevervine plant or local warming's infusion; More preferably this solvent be concentration at straight alcohol or the ethanol water of 25～100wt%, best concentration of alcohol is 70～100wt%; Best is that alcohols solvent is the acid alcohol that contains hydrochloric acid.
Preferably, extract this red Chinese fevervine stem and root and be to use acid alcohol, in red Chinese fevervine weight and acid alcohol volume are about 1: 5 to 1: 20 proportion, under room temperature, carry out.
The present invention be relevant to a kind of be used to the medical composition that improves metabolic syndrome, it comprises red Chinese fevervine extract and pharmaceutically acceptable supporting agent or excipient, and wherein the preparation process of this red Chinese fevervine extract comprises: red Chinese fevervine plant or part that fresh or drying are provided; With this red Chinese fevervine plant of solvent extraction or local to obtain solvent extract; This solvent extract of concentrate drying; Obtain red Chinese fevervine extract; And this red Chinese fevervine extract is added pharmaceutically acceptable supporting agent or excipient is made medical composition, with as the usefulness of improving metabolic syndrome.
Preferably, the red Chinese fevervine part that provides is stem and root.
Preferably, the solvent that extracts this red Chinese fevervine plant or part can be water, alcohols, alcohol solution and ethyl acetate, wherein water still can carry out red Chinese fevervine plant or local extraction under the temperature that raises, it is the medicine kettle that water can be put into general infusion Chinese medicine, directly with red Chinese fevervine plant or local warming's infusion; More preferably this solvent be concentration at straight alcohol or the ethanol water of 25 ~ 100wt%, best concentration of alcohol is 70 ~ 100wt%; Best is that alcohols solvent is the acid alcohol that contains hydrochloric acid.
Preferably, extract this red Chinese fevervine stem and root and be to use acid alcohol, in red Chinese fevervine weight and acid alcohol volume are about 1: 5 to 1: 20 proportion, under room temperature, carry out.
The present invention is relevant to a kind of suitable metabolic syndrome patient's food compositions simultaneously, and it is to comprise red Chinese fevervine extract, and its preparation process comprises: red Chinese fevervine plant or part that fresh or drying are provided; With this red Chinese fevervine plant of solvent extraction or local to obtain solvent extract; This solvent extract of concentrate drying; Obtain red Chinese fevervine extract; And the food prepared therefrom composition, with as the usefulness of improving metabolic syndrome.
Preferably, the red Chinese fevervine part that provides is stem and root.
Preferably, the solvent that extracts this red Chinese fevervine plant or part can be water, alcohols, alcohol solution and ethyl acetate, wherein water still can carry out red Chinese fevervine plant or local extraction under the temperature that raises, it is the medicine kettle that water can be put into general infusion Chinese medicine, directly with red Chinese fevervine plant or local warming's infusion; More preferably this solvent be concentration at straight alcohol or the ethanol water of 25～100wt%, best concentration of alcohol is 70～100wt%; Best is that alcohols solvent is the acid alcohol that contains hydrochloric acid.
Preferably, extract this red Chinese fevervine stem and root and be to use acid alcohol, in red Chinese fevervine weight and acid alcohol volume are about 1: 5 to 1: 20 proportion, under room temperature, carry out.
Because step of the present invention is simple; and need not the red Chinese fevervine extract that special solvent can extract available energy; therefore with low cost; and at sugar solution ferment in the enteron aisle; acetyl-coa carboxylase ferment (Acetyl-CoA carboxylase in the liver; ACC) experimentize after; confirmation truly has the effect of inhibition; and can promote acyl-CoA oxidation ferment (Acyl-CoAoxidase in the liver; ACO) activity; therefore; the present invention only need utilize single red Chinese fevervine extract, the function that can reach regulating blood lipoid simultaneously and regulate blood sugar, and have the effect of improving metabolic syndrome.
Description of drawings
The present invention is further described below in conjunction with drawings and Examples.
Fig. 1 is preparation flow figure of the present invention.
Fig. 2 is the linear graph that red Chinese fevervine acid alcohol extract of the present invention suppresses the activity of acetyl-coa carboxylase ferment.
Fig. 3 (A) utilizes the clofibrate of various dose to promote the block diagram of the activity of acyl-CoA oxidation ferment.
Fig. 3 (B) utilizes the red Chinese fevervine alcoholic extract of the present invention 100 μ g/ml to promote the block diagram of the activity of acyl-CoA oxidation ferment.
The number in the figure explanation:
(a) provide red Chinese fevervine
(b) the extraction red Chinese fevervine is to obtain extract
(c) concentrate drying extract (d) obtains red Chinese fevervine extract
(e) preparation medical composition (f) preparation food compositions
The specific embodiment
Please referring to shown in Figure 1, the present invention is relevant to a kind ofly be used to the red Chinese fevervine extract that improves metabolic syndrome, and it is prepared with following method, and it comprises provides the red Chinese fevervine of fresh or drying plant or part (a); With this red Chinese fevervine plant of solvent extraction or local to obtain solvent extract (b); This solvent extract of concentrate drying (c); And obtain this red Chinese fevervine extract (d).Still can comprise the food compositions (f) that preparation contains the medical composition (e) of this red Chinese fevervine extract or comprises this red Chinese fevervine extract afterwards.
The present invention at first utilizes solvent that the red Chinese fevervine plant of fresh or drying is extracted, utilize solvent with the contained active component stripping in addition that dissolves in the solvent in the red Chinese fevervine, and further utilize this to dissolve in the interior active component of solvent, handle the ferment in enteron aisle sugar solution ferment and the liver, find that it can regulate blood sugar and blood fat is regulated and control crucial enzyme activity.In specific embodiments of the invention, can find to utilize this active component that dissolves in the solvent to have the activity that suppresses enteron aisle sugar solution ferment, liver acetyl-coa carboxylase ferment, and can promote the activity of liver cell acyl-CoA oxidation ferment.Therefore, the red Chinese fevervine extract that the present invention obtains can further cooperate pharmaceutically acceptable supporting agent or excipient, to make the treatment medical composition of metabolic syndrome or relevant health food etc.
" red Chinese fevervine " of indication of the present invention be the weighting platform gulf fleece-flower root (Taiwan TuberFleeceflower) again, its original producton location is Taiwan, be the Taiwan autochthonous species, do not have tangible piece root, being the mutation of traditional fleece-flower root, is a kind of of Chinese medicine medicinal material also, is common in low altitude mountainous area in the full island, Taiwan, grow in roadside and border more, but do not have the people that the alternative traditional fleece-flower root of the rhizome portion of the Taiwan fleece-flower root is used at present.Red Chinese fevervine is to belong to Polygonum (Polygonum), polygonaceae (Polygonaceae), and its formal name used at school can be Polygonum multiflorum Thunb.Ex murrayvar.hypoleucum (Ohwi) Liu, Ying ﹠amp; Lai, Pleuropterus hypoleucusmultiflorum, Polygonum hypoleucum Ohwi and Polygonum multiflorumThunb.var.hypoleucum (Ohwi) Liu, Ying ﹠amp; Lai, the part of its root and rattan (stem) can antibechic, timid wind, timid phlegm, treatment cold cough rheumatism etc.; And flu, external application knife wound etc. can be treated by leaf portion.
" metabolic syndrome (the metabolic syndrome) " of indication of the present invention means insulin impedance syndrome (insulin resistance syndrome), symptom such as its concurrent hypertension of second diabetes mellitus type that refers to produce because of obesity, high fat of blood.And insulin resistance (insulinresistance) should be the common favourable turn that causes a disease that these diseases are therefore gathered together, and it is can enteron aisle sugar be separated ferment, suppresses liver acetyl-coa carboxylase ferment, promoted liver acyl-CoA oxidation ferment by suppressing, control blood sugar, serum lipid concentrations and be enhanced.
" red Chinese fevervine extract " of indication of the present invention means and utilizes solvent that the red Chinese fevervine plant is extracted, to obtain the active component that dissolves in solvent in the red Chinese fevervine; This solvent can be water, alcohols such as ethanol, alcohol solution and ethyl acetate etc., and ethanol is preferably acid alcohol.In the present invention, this red Chinese fevervine plant can be complete stool plant or the part of fresh or drying; The part can be red Chinese fevervine leaf portion, red Chinese fevervine stem or red Chinese fevervine root etc.; At preferable red Chinese fevervine stem and the root of referring to of the present invention.
" concentrate drying " of indication of the present invention is to use decompression concentration method, freeze-drying and other to have the concentrate drying method of knowing usually known to the knowledgeable in this area.
" the pharmaceutically acceptable supporting agent " of indication of the present invention; if medical composition is oral pattern; then supporting agent is that to protect this medical composition not digested before no show active position still be the decomposition of system, and loses effectiveness, and/or can promote release and the effect of medicine.
" the pharmaceutically acceptable excipient " of indication of the present invention is for the uniformity that increases medicine, the stability excitant, bad smell etc. with the minimizing medicine, has the excipient of knowing usually known to the knowledgeable in this area as sugar alcohol, starch or other.
Red Chinese fevervine extract of the present invention, it is to be made into the medical composition that improves metabolic syndrome, and can give different forms, include but not limited to the orally give form, it comprises the red Chinese fevervine extract of independent novel form or is mixed with pharmaceutically acceptable supporting agent that the form of orally give can be selected from and comprise in the group that is made up of lozenge, powder, suspension, chyle liquid, capsule, particle, tablet, pill, liquid, spirits and medicated syrup.
Following examples are in order to specify technological means of the present invention, and will carry out activity analysis after red Chinese fevervine extract of the present invention and the specific ferment effect.
The preparation of embodiment 1. red Chinese fevervine extracts
The enzyme alcoholic extract:
After red Chinese fevervine ground with grater, extract with acid alcohol (being added with concentration 0.1% hydrochloric acid (HCl)), the ratio of the weight of red Chinese fevervine and the volume of acid alcohol is about 1: 8 (W/V=1/8), it is placed leave standstill in the room temperature or extract with the available 1000 speed concussions of changeing (rpm).
After 72 hours, do preliminary filtration with gauze, the filtrate of gained is changeed centrifugal 15 minutes of the speed of (rpm) again with per minute 6000, and getting upper strata liquid concentrate drying becomes the acid alcohol extract, and its extraction yield is about 6.9%.
This extract is redissolved, making its concentration is 50mg/ml again.
Water hot extraction's thing
Red Chinese fevervine was put into the infusion of Chinese medicine kettle about two hours, and the weight of red Chinese fevervine and the volume ratio of water are about 1: 20 (W/V=1/20), and again with filtered through gauze, this filtrate obtains water hot extraction's thing in cryodesiccated mode, and its extraction yield is about 9.2%.
Again this extract is redissolved, make its concentration the same with the acid alcohol extract for 50mg/ml, to carry out following experiment.
Embodiment 2. red Chinese fevervine extracts inhibition alpha-glucosidase (α-glucosidase)
(p-nitrophenyl α-D-glucoside pNP-G) is subjected to the matter analytic approach to suppress the analysis of alpha-glucosidase activity with p-nitrophenyl-alpha-D-glucose glycosides.Its method is to comprise:
1. at first with phosphate buffer solution (100mM, pH7.0) alpha-glucosidase (EC 3.2.1.20, G5003, Sigma-Aldrich Co., USA) solution and the 5x10 of configuration 1.U/ml
-3(Sigma-Aldrich Co. USA) is subjected to the matter solution for standby to M p-nitrophenyl-α-D-glucopyranoside for p-nitrophenyl α-D-glucopyranoside, pNP-G.
2. subsequently in each hole of 96 porose discs, phosphate buffer solution (the 0.4M that adds 40 microlitres (μ l) in regular turn, pH7.0), the alpha-glucosaccharase enzyme solutions of 10 microlitres (μ l), and different dilution red Chinese fevervine extract 50 microlitres (μ l), add deionized water to 170 microlitre (μ l) again.
3. allow incubator preheating after 5 minutes in 37 ℃ environment, what add 30 microlitres (μ l) preheating is subjected to matter solution, and after fully concussion mixed, effect was 10 minutes under 37 ℃ environment.
4. again with wavelength 405 photometric analyzer of rice (nm) (Power Wave XS, Bio TekCo., the USA) light absorption value before and after the record, and use IC how
50Expression suppresses 50% the required concentration of alpha-glucosidase activity.
Please referring to table 1, it is to show with acid alcohol extract and the resulting result of water hot extraction's thing:
Table 1.
Can learn that by table 1 acid alcohol of red Chinese fevervine and water hot extraction's thing all have the effect of inhibition for the activity of alpha-glucosidase, be good with the effect of acid alcohol extract gained again wherein.
Utilize rat intestinal ferment liquid (the rat intestinal acetone powder that slightly comes together, SigmaChemical Co., St.Louis, Mo.) be the source that sugar is separated ferment, give different carbohydrates and be subjected to matter (maltose, sucrose and starch), with the inhibitory action of test acid alcohol red Chinese fevervine extract for different sugar solution enzyme activity.
Please referring to table 2, it is to show to utilize acid alcohol red Chinese fevervine extract for the inhibition situation of different sugar solution enzyme activity that it also uses IC
50Represent the active situation that suppresses:
Table 2.
Shown by above-mentioned table 2, the acid alcohol extract is separated ferment (maltase), starch sugar solution ferment for maltose sugar, and (α-amylase) and sucrose sugar are separated the effect that ferment (sucrase) all has inhibition, and inhibition strength is that maltose sugar is separated ferment＞starch sugar solution ferment＞sucrose sugar and separated ferment.
The preparation of the fat-soluble extract of embodiment 4. red Chinese fevervines
After red Chinese fevervine ground with grater, methanol aqueous solution extraction with concentration 50wt%, the volume ratio of the weight of red Chinese fevervine and the methanol aqueous solution of 50wt% is 1: 10 (W/V=1/10), the concussion extraction was filtered with adhesive-bonded fabric after 24 hours at room temperature, the filtrate of gained adds isopyknic ethyl acetate (ethyl acetate)/water, carry out liquid-liquid extraction after shaking mixing, get the ethyl acetate layer on upper strata, add and ethyl acetate isopyknic 5wt% sodium acid carbonate (Sodium Bicarbonate, NaHCO again
3) shake mixing, remove the sodium acid carbonate layer again, with the flush away free fatty, then again behind the concentrate drying ethyl acetate layer, can obtain desired fat-soluble extract, extraction yield is about 0.91%.
(Dimethyl Sulfoxide DMSO) redissolves this fat-soluble extract, and making its concentration is 10mg/ml with dimethyl sulfoxide (DMSO).
Acetyl-coa carboxylase ferment (ACC) is the synthetic crucial ferment of aliphatic acid; its product malonyl coacetylase (malonyl-CoA) is aliphatic acid NOS (fatty acid synthase; FAS) be subjected to matter; and be plain palmitoyl transferase (the carnitin palmitoyl CoA transferase of carnic acid; CPT) inhibitor; therefore suppressing ACC, not only can to suppress endogenous lipid synthetic, also can accelerate an oxidation of line body fat acid.
From mouse liver purifying ACC and test, its step is method (the Tanabe T. with reference to people such as Tanabe, Nakanishi, S., Hashimoto T., Ogiwara, H., Nikawa J., Numa be carboxylase from rat liver S.1981.Acetyl-CoA, Meth.Enzymol.71 (pt C): 5-16).Result such as the table 3 of gained:
Table 3.
Can be found out that by above-mentioned table 3 acid alcohol of red Chinese fevervine and fat-soluble extract all have good ACC and suppress active.Get the acid alcohol extract again and carry out IC
50Measure.Please referring to shown in Figure 2, it is the concentration of red Chinese fevervine acid alcohol extract and the linear change of inhibition, and figure can learn that red Chinese fevervine acid alcohol extract reaches the active desired concn (IC of ACC of inhibition 50% thus
50) be 0.04mg/ml.
Embodiment 6. red Chinese fevervine acid alcohol extracts promote acyl-CoA oxidation ferment (Acyl-CoA oxidase, activity analysis ACO)
Acyl-CoA oxidation ferment (ACO) is the crucial ferment of aliphatic acid beta-oxidation effect, also is the typical downstream gene of peroxisome proliferator-activated receptor alpha (PPAR α), can be as the index of fatty acid oxidation metabolic activity.
This analysis is to inoculate the H4IIEC3 liver cell in 12 porose discs with suitable density, it is attached to wait to lie prone, be replaced by contain variable concentrations known and can promote the clofibrate (clofibrate) of ACO performance or the red Chinese fevervine acid alcohol extract of 100 microlitre/milliliters (μ l/ml), cultivate appropriate time, collecting cell behind the nutrient solution is removed in suction, carries out active testing at ACO.
The ACO active testing is the methods of scholar in research periodical (Small GM.Burdett K.ConNock MJ.1985.A sensitive spectrophotometric assay forperoxisomal acyl-CoA oxidase.Biochemical Journal.227:205-210.) such as employing Small.Please referring to shown in Figure 3, wherein Fig. 3 (A) is that expression utilizes the clofibrate of variable concentrations (100 μ M, 500 μ M, 1000 μ M) in following degree that can promote the ACO activity of different time (48 and 72 hours) effect; And Fig. 3 (B) is the active multiple that increases of the ACO of red Chinese fevervine acid alcohol extract processing after 48 hours of representing to utilize 100 μ l/ml.
Embodiment 7. red Chinese fevervine acid alcohol extracts bring out the influence of metabolic syndrome C57BL/6J mouse for high fat
The C57BL/6J mouse earlier fed for eight weeks with the high lipid food of the cream that contains 30% (g/g), made metabolism syndrome symptoms such as obesity, high fat of blood, hyperinsulinemia occur.This mouse that the metabolic syndrome symptom occurs is divided into two groups arbitrarily, (HF+No.11), it is control group (HF) that another group is kept high lipid food to one group of high lipid food (being equivalent to the red Chinese fevervine acid alcohol extract (1500mg/kg body weight) that mouse body weight per kilogram gives 1500 milligrams) that is added with the red Chinese fevervine acid alcohol extract of 3% (g/g).Other has normal feed control group (LF).Behind five all feedings, (oral glucose tolerance test, OGTT), its result as shown in Table 4 to carry out the oral glucose tolerance test.Sacrifice mouse afterwards, its final body weight and blood biochemical analysis result are as shown in Table 5.
When table 4. the 5th all oral glucose tolerances were tested, each organized the variation of mouse blood sugar
The result represents with mean value ± standard deviation value (SD).The different letter representatives of subscript there were significant differences property, P＜0.05.
Table 4 shows, through being provided with red Chinese fevervine acid alcohol extract five weeks, can significantly reducing high fat and bring out metabolic syndrome C57BL/6J mouse fasting serum glucose (0min); And make its under 60 and 90 minutes blood sugar of oral glucose tolerance property testing and 2 hours blood glucose curves area (areaunder curve, AUC) higher fat control group person is low significantly.Show that red Chinese fevervine acid alcohol extract not only can reduce fasting serum glucose, and can improve the glucose tolerance.
Table 5. feeding five all red Chinese fevervine acid alcohol extracts bring out the influence of metabolic syndrome C57BL/6J mouse body weight and serum physical signs for high fat
Shown numerical value is mean value ± standard deviation value (SD).
Analyzing by statistics, is benchmark with P value (P-value)＜0.05.
Subscript a, b, c representative there were significant differences property.
Table 5 shows that the red Chinese fevervine acid alcohol extract that gives 1500mg/kg body weight can reduce significantly because of leptin (leptin) concentration in the caused high cholesterol of high fat diet, high triglyceride, hyperinsulinism secretion and the blood.
The present invention is made with simple preparation method; and need not to utilize special solvent or complicated extraction step to reach; and with after the multinomial test of red Chinese fevervine extract process that method of the present invention was extracted; provable its has the function of regulating blood sugar, blood fat; can suppress sugar again and separate the activity of ferment and acetyl-coa carboxylase ferment; also can promote the activity of acyl-CoA oxidation ferment, therefore can improve metabolic syndrome, the big Gospel of for the metabolic syndrome patient, can saying so.
The above, it only is preferred embodiment of the present invention, be not that the present invention is done any pro forma restriction, have in the technical field under any and know the knowledgeable usually, if in the scope that does not break away from technical characterictic that the present invention carries, utilize disclosed technology contents to do the local equivalent embodiment that changes or modify, and do not break away from technical characterictic content of the present invention, all still belong in the scope of the technology of the present invention feature.
Claims (3)
1. red Chinese fevervine extract, it is characterized in that: it is made by the method that may further comprise the steps:
Red Chinese fevervine stem and the root of fresh or drying are provided;
Extract this red Chinese fevervine stem and root to obtain the alcohols solvent extract with alcohols solvent;
This alcohols solvent extract of concentrate drying; And
Obtain red Chinese fevervine extract; Wherein, described alcohols solvent system is more than or equal to 25wt% and less than the ethanol water and the acid alcohol that contains hydrochloric acid of 100wt%, and red Chinese fevervine weight and acid alcohol volume are in 1: 5 to 1: 20 the proportion, to carry out under room temperature; This red Chinese fevervine extract is applicable to improve and comprises that suppressing enteron aisle sugar separates ferment, inhibition liver acetyl-coa carboxylase ferment, promotes liver acyl-CoA oxidation ferment, the metabolic syndrome of regulating blood sugar concentration and regulating serum lipid concentrations.
2. a medical composition that is used for improving metabolic syndrome is characterized in that comprising red Chinese fevervine extract and pharmaceutically acceptable supporting agent or excipient, and wherein preparation process comprises:
Red Chinese fevervine plant or the part of fresh or drying are provided;
Extract this red Chinese fevervine plant or local to obtain solvent extract with alcohols solvent;
This solvent extract of concentrate drying;
Obtain red Chinese fevervine extract;
Red Chinese fevervine extract is added pharmaceutically acceptable supporting agent or excipient, to obtain medical composition; Wherein said alcohols solvent is to be more than or equal to 25wt% and less than the ethanol water of 100wt% and the acid alcohol that contains hydrochloric acid, and red Chinese fevervine weight and acid alcohol volume are in 1: 5 to 1: 20 the proportion, to carry out under room temperature; Described metabolic syndrome is to comprise that suppressing enteron aisle sugar separates ferment, inhibition liver acetyl-coa carboxylase ferment, promotes liver acyl-CoA oxidation ferment, the metabolic syndrome of regulating blood sugar concentration and regulating serum lipid concentrations.
3. a suitable metabolic syndrome patient food compositions, it is characterized in that: comprise red Chinese fevervine extract, its preparation process comprises: red Chinese fevervine plant or part that fresh or drying are provided;
Extract this red Chinese fevervine plant or local to obtain solvent extract with alcohols solvent;
This solvent extract of concentrate drying;
Obtain red Chinese fevervine extract; Wherein, described alcohols solvent system is more than or equal to 25wt% and less than the ethanol water and the acid alcohol that contains hydrochloric acid of 100wt%, and red Chinese fevervine weight and acid alcohol volume are in 1: 5 to 1: 20 the proportion, to carry out under room temperature; Described metabolic syndrome is to comprise that suppressing enteron aisle sugar separates ferment, inhibition liver acetyl-coa carboxylase ferment, promotes liver acyl-CoA oxidation ferment, the metabolic syndrome of regulating blood sugar concentration and regulating serum lipid concentrations.
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