TH9821U - A method for preparing Lagerstroemia speciosa flower extract containing high levels of phenolic compounds, flavonoids, and anthocyanins, with inhibitory activity on collagen-degrading enzymes. - Google Patents

Abstract

DC60 (June 27, 2014) This report describes a method for preparing a high-phenolic, flavonoid, and anthocyanin-rich extract of Lagerstroemia speciosa (Indian crape myrtle) flowers with collagen-degrading enzyme inhibitory activity. The extraction process involved separating high concentrations of phenolic compounds, flavonoids, and anthocyanins from Lagerstroemia speciosa petals using organic solvent fermentation mixed with water. The extract was then dried at low temperatures using a combination of refrigerated and ultra-cold evaporation under vacuum conditions. The resulting extract is a dry, purplish-brown powder that is readily soluble in both water and alcohol. A previously unreported activity was discovered: the inhibition of matrix metalloproteinase enzymes that degrade collagen. This makes the extract suitable for use as a key ingredient in skincare products or for applications involving collagen breakdown disorders. This invention not only supports the utilization of Thai herbs but also adds value to Thai flowers, which are not only used as ornamental plants but also offer potential for medicinal use in skincare product development. This method for preparing a high-phenolic, flavonoid, and anthocyanin-rich extract of Lagerstroemia speciosa (Indian crape myrtle) flowers, exhibiting collagen-degrading enzyme inhibitory activity, involves extracting these important phenolic compounds from the petals using an organic solvent fermentation method mixed with water. The extract is then starched at low temperatures and subjected to high-temperature evaporation and vacuum drying. The resulting extract is a dry, purplish-brown powder that is readily soluble in both water and alcohol. A previously unreported activity was discovered: the inhibition of matrix metalloproteinase enzymes that degrade collagen. This makes the extract suitable for use as a key ingredient in skincare products or for applications involving collagen degradation disorders. This invention not only supports the utilization of Thai herbs but also adds value to Thai flowers, which are not only used as ornamental plants but also offer potential medicinal uses in skincare product development.

Claims (1)

1. The preparation method for an extract of Lagerstroemia speciosa flowers containing high levels of phenolic compounds, flavonoids, and anthocyanins, and exhibiting collagen-degrading enzyme inhibitory activity, involves the following steps: a. Dry the purplish-red petals of Lagerstroemia speciosa flowers at 45°C for 2 days and grind them finely using a dry grinder until the particle size reaches 200-250 microns. b. Soak the dried petal powder from step a in a 1:1 ratio of distilled water and alcohol (1:18 ratio), shaking well at 20-25°C in an opaque container until the powder fully swells. Then, ferment the mixture, stirring periodically for 20 minutes each time, 15-20 times a day, for 5 days using a magnetic stirrer. c. Filter the fermented portion from step b through No. 1 filter paper. d. Evaporate the alcohol from the extract filtered through step c using a vaporizer. The extract was then evaporated using a rotary evaporator at 45°C for 6-10 hours until it thickened and reduced to 1% of the total solution. The resulting fraction was then cryopredried at -50°C for 1-2 days until a completely dry purple powder was obtained, yielding 22%. This extract is highly soluble in both water and alcohol.