TH96816B - Production of bioactive fractions with antimutagenic, antioxidant, and anti-arthritis activity from crude extract of longan. - Google Patents

Abstract

-----10/08/2021------ No abstract of the invention ------23/07/2020------(OCR) This invention concerns the production of bioactive fractions with antimutagenic, antioxidant, and anti-degradation activity from crude extract of longan, which is ethyl acetate-soluble, using a solvent that leaves no toxic residue and is not harmful to the body. The preparation process involves drying whole longan fruit in a hot air oven and removing only the dried seeds, or drying the seeds removed from drying golden longan flesh. The extracts are then processed with hot water followed by ethyl acetate, yielding fractions containing important compounds in the polyphenol group, which exhibit antioxidant activity and inhibit the degradation of cartilage components. ------------ DC60 (07/01/2016) This invention concerns the production of bioactive fractions with antimutagenic activity... This invention concerns the production of bioactive fractions with mutagenic, antioxidant, and anti-arthritis activity from crude extract of longan, which is ethyl acetate-soluble, using a non-toxic and harmless solvent. The preparation method involves drying whole longan fruit in a hot air oven and removing only the dried seeds, or drying the seeds removed from drying golden longan flesh. These are then extracted with hot water followed by ethyl acetate, yielding fractional extracts containing important polyphenols with antioxidant activity and inhibitory effect on cartilage degradation. (Summary of the invention revised 07/01/59) The extract is then processed with hot water followed by ethyl acetate, yielding a fractional extract containing important polyphenols with antioxidant properties and the ability to inhibit the degradation of cartilage components. ---------------------------------------------------------------This invention relates to a method for preparing extracts from dried longan (Euphoria longana), specifically the ethyl acetate-soluble fraction, using a solvent that leaves no toxic residue and is not harmful to the body. The preparation method involves drying whole longan fruit in a hot oven and removing only the dried seeds, or drying the seeds removed from the golden longan flesh. The seeds are then extracted with hot water followed by ethyl acetate, yielding a fractional extract containing important polyphenols with antioxidant properties and the ability to inhibit the degradation of cartilage components.

Claims (3)

OCR 02/10/2566 1. The production of bioactive fractions with antimutagenic, antioxidant, and anti-arthritis activity from crude longan extract involves the following steps: a. Preparation of dried longan seed powder: Leftover longan seeds from drying golden longan flesh are washed, dried in a hot-air oven, and then ground into a fine powder. The resulting dried longan seed powder is stored at -10 to -20 degrees Celsius. b. Hot water extraction: The dried longan seed powder from step a is mixed with water and boiled for 30 minutes to 4 hours. The mixture is then stirred overnight at room temperature, filtered, and the solids are discarded. The resulting water-soluble solution is then boiled again until the volume of the crude extract is reduced to 1/10th to 1/20th of the volume of the starting solution. A crude extract solution will be obtained from dried longan seed powder. C. Ethyl acetate extraction method - Mix the crude extract solution obtained from step B with ethyl acetate solvent in a ratio of 1:1 to 1:4 by volume to volume for 0.5 to 1 hour. Separate the ethyl acetate-soluble solution from the insoluble solution. The ethyl acetate-soluble solution from the first extraction is stored, and the insoluble solution is kept for use in the next extraction step. - The insoluble solution from the previous extraction step is then extracted again with ethyl acetate, and the ethyl acetate-soluble solution is separated from the insoluble solution. The ethyl acetate-soluble fraction from the second extraction was collected, while the ethyl acetate-insoluble fraction was kept aside. The ethyl acetate-soluble solutions from the first and second extractions were combined and dried using a vacuum evaporator at 45-55°C for 30 minutes to 1 hour. The mixture was then completely freeze-dried to obtain an ethyl acetate-soluble extract in the form of a dry brown powder. This extract contains polyphenols, which exhibit antioxidant activity and inhibit the degradation of cartilage components. 2. Production of bioactive fractions with antimutagenic, antioxidant, and anti-arthritis activity from crude longan extract, as per claim 1, whereby step A involves drying in a hot-air oven at a temperature of 90-100°C for 18-24 hours. 3. Production of bioactive fractions with antimutagenic, antioxidant, and anti-arthritis activity from crude longan extract, as per claim 1, where in step b, the mixing ratio of dried longan seed powder to water is between 1:5 and 1:10 by weight/volume.