TH5339C3 - The preparation process of Gotu Kola extract that has antioxidant activity. - Google Patents

Abstract

DC60 (30/10/52) This invention involves the preparation of Centella asiatica extract, which is dissolved in ethyl acetate. The non-toxic, non-toxic solvent was used for this preparation by drying the gotu kola with a freeze drier and extraction with ethyl alcohol. Xane and ethyl acetate Will extract in A group of polyphenols and flavonoids (flavonoids) which have antioxidant activity. The invention involved the preparation of Centella asiatica extract, which was soluble. In ethyl acetate The non-toxic, non-toxic solvent was used for this preparation by drying the gotu kola with a freeze drier and extraction with ethyl alcohol. Xane and ethyl acetate Will extract in A group of polyphenols (Polyphenol) and flavonoids (Flavonoids), which have antioxidant activity.

Claims (6)

1. Preparation process of Gotu Kola extract that has antioxidant activity. Contains steps a) The process of preparing Gotu Kola powder The various parts of Gotu Kola are thoroughly washed in water and then dried by sublimation machine, dried under pressure. The temperature was between -40 and -60 degrees Celsius, then ground into a powder. B) The extraction process with ethyl alcohol. The dry powder gotu kola mixed with ethyl alcohol solution in water at a concentration of 50 to 100 percent with the ratio of dry powder gotu kola. The ethyl alcohol solution in water ranged from 1 to 5 to 1: 10 by weight by volume for 4 to 24 hours. Dry with vacuum evaporator At temperatures between 45 and 55 ° C until the remaining volume of the coarse extraction solution was approximately 1 part in 10 to 1 part in 20 parts of the precursor solution c) hexane extraction process. By using the coarse extraction solution obtained from extraction with ethyl al Gohol is mixed with hexane solvent in a ratio of 1: 1 to 1: 10 by volume by volume. Soak for 0.5 to 1 hour and separate the dissolved solution. In hexane leaves the insoluble part in hexane Then the solution is not. Dissolved in hexane and extracted with hexane again And separate only the dissolved solution In hexane leaves the insoluble part in hexane Take the solution of the dissolved in heparin. Both timescane were combined. D) Ethyl acetate extraction process. By mixing the insoluble part in hexane with Ethyl acetate solvent In the ratio of the insoluble hexane to ethyl acetate solution was 1: 1 to 1: 4 v / v for 0.5 to 1 h and separated the dissolved ethyl acetate from Solution part no Dissolved in ethyl acetate Extract the insoluble part of ethyl acetate as well. Ethyl acetate repeat one more time And separated the dissolved part of ethyl acetate from Insoluble fraction solution in ethyl acetate Then the solution of the dissolved in ethyl. The cite that had both been combined Then dried with a vacuum dry evaporator at Temperature between 45-55 degrees Celsius for 30 to 60 minutes will get rough extract from Gotu Kola. 2. Preparation process of Gotu Kola extract According to claim 1, where the portion of Gotu Kola used for selective extraction from the root, stem and any part of the leaf Or a combination of two or more parts 3. Preparation process of Gotu Kola extract According to claim 1, where the concentration of the solution Ethyl alcohol in the extraction process with ethyl alcohol The most suitable is 70 percent 4. Preparation process of Gotu Kola extract According to claim 1, where the ratio between gotu kola powder The optimum ethyl alcohol solution was 1 by 10 by weight per volume. 5. Preparation process of Gotu Kola extract According to claim 1, where the ratio between solutions The coarse extract dissolved in ethyl alcohol per hexane in the hexane extraction process at The most suitable is 1 to 1 by volume by volume. 6. Preparation process of Gotu Kola extract According to claim 1, where the ratio between solutions The insoluble in hexane per ethyl acetate at the optimum ethyl acetate extraction step was 1: 1 v by v.