TH95082A - A method for producing L-amino acids using bacteria of the family. Enterobacteriaceae - Google Patents

Abstract

DC60 (15/10/50) This invention discloses methods for producing L-Amino acids are described, for example L-threonine, L-lysine, L- Histidine, L-phenylalanine, L-arginine, L-tryptophan, L-glutamic acid using bacteria of the family Enterobacteriaceae, whereby the bacteria have been modified To achieve the predominance of the function of the original alcohol dehydrogenase transcribed by the adhE gene or the alcohol dehydrogenase mutant resistant to the inactivity of oxygen.

Claims (1)

Disclaimer (all) which will not appear on the advertisement page. : ------ 23/03/2018 ------ (OCR) Page 1 of Number 2 Page Terms 1. Method for L-Amino Acid Production by Compound Fermentation. Include: A) Culture in ethanol-containing media of L-A-producing bacteria Mino of the clan Enterobacteriaceae Alcohol dehydrogenase, and B) L-amino acid isolation from the f-diacromatic medium, where the genes that decipher alcohol dehydrogenase are expressed under the control Of non-natural stimuli acting under the conditions of oxygen-rich fertilization 2. Method according to claim 11, where such unnatural activators were selected from the group containing ptac, Plac, Ptrp, ptr0, PR, and PL 3. Method according to claim 1 or 2, where alcohol dehydrogenase is resistant to Dehydration in the presence of oxygen 4. One of the methods of claim \ 'teng 3, whereby alcohol dehydrogenase Gluten is of bacterial origin, selected from a group containing Escherichia coli, Erwinia carotovora, Salmonella typhlmurium, Shigella flexneri, Yersenia pestis, Pantoea ananatis, Lactobacillus plantarum, and Lactococcus lactis. 5. Rights 1 to 4, where alcohol dehydrogenase This contains the amino acid assortment given in the risk: 2, except the glutamic acid set at position 568 has been replaced with another amino acid set apart. From the set of aspartic acid 6. Method according to any one of claim 1 to 4, where alcohol dehydrogenase is found The amino acid assortment provided is composed of: 2, except the \ 'glutamic acid series at position 568 has been replaced with the lysine series. 7. Method according to claim 5 ! 6, where alcohol dehydrogenase is said to have an At least one additional species in which the bacteria are responsible for the growth of bacteria, as mentioned in Liquid medium in which ethanol is the only carbon source 8. Method according to claim 7, where the additional mutations are selected from a group containing: A) the substitution of the acidic series. Glutamic acid at position 560 in sampling: 2 with another amino acid series B) substitution of phenylalanine at position 566 in collation: 2 with series. Another amino acid complex 30 page 2 of the number 2 C) replacement of glutamic acid series, methionine series, tyrosine series, isosousine series, and The alanine series at positions 22, 236, 461, 554, and 786, respectively, in the arrangement of: 2. 9. Methods according to claim 7, where such additional mutations are selected from a group containing: A) Substitution of Glutamic acid set at position 560 in arrangement: 2 With lysine series B) replacement of \ 'phenylalanine series at position 566 in the arrangement: 2 with the valine series C) replacement of glutamic acid series, series Methionine, tyrosine series, isoleucine series, and alanine series at positions 22, 236,461,554, and 786, respectively, in sequencing: 2. With glycine series, valine series, cysteine series, sherry series, and valine series, respectively, and a) their combination 1 0. Method According to any one of claim 1 to 9, where L-amino acid-producing bacteria These belong to the genus chosen from the group consisting of Escherichia, Enterobacter, Erwinia, Klebsiella, Pantoea, Providencia, Salmonella, Serratia, Shigella, and Morganella. 1. Methods according to one of the claims 1 to 10, Where L - amino acids are Choose from a group consisting of L-threonine, L-sysine, L-histidine, L-phenylalanine, L-arginine, L-. Tryptophan, L-Glutamic Acid, and L-Lucine ------------ 1. Methods for the production of L-Amino acids containing: A) Culture in culture medium containing bacterial ethanol of the family Enetrrobacteriaceae The separation of L-amino acids from the culture medium, by the genes that decipher the alcohol dehydrogenase, was expressed under control. Of unnatural activators where under aquaculture conditions 2. Method of claim 1, where such unnatural activators were selected from the aquaculture group. Contains Ptac, Ptac, Ptrp, Ptrc, PR, and PL 3. Method of claim 1 or 2, whereby alcohol dehydrogenase is resistant to Oxygen dehydrogenase 4. Any method of claim 1 or 3, whereby alcohol dehydrogenase They are of bacterial origin, selected from groups containing Escherichia coli, Erwinia carotovora, Salmonella typhimurium, Shigella flexneri, Yersenia pestis, Pantoea ananatis, Lactobacillus plantarum, and Lactococcucus lactis. Rights 1 to 4, where alcohol dehydrogenase is loud The amino acid assay was composed of: 2, except the glutamic acid set at position 568 was replaced by an amino acid series other than the acidic series. Aspartic 6. Method for any one of Claims 1 to 4, where alcohol dehydrogenase as The amino acid assortment provided is composed of: 2, except the glutamic acid set at position 568 has been replaced by the lysine package. 7. Method according to claim 5, or 6, where the alcohol dehydrogenase is At least one additional cultivar that can improve the growth of such bacteria in Liquid medium in which ethanol is the only carbon source 8. Method of claim 7, where such additional mutations are selected from a group consisting of: A) the substitution of the acidic series. Glutamic acid at position 560 in collation: 2 with another amino acid series. B) The replacement of the phenylalanine set at position 566 in the: 2 alignment with a different amino acid series. Another amino acid C) replacement of glutamic acid series, methionine series, tyrosine series, isoleucine series, and alanine series at the 22,236,461,554, and 786, respectively, in the second order with another amino acid series, and D) their combination 9. Methods according to claim 7, where additional mutations These were selected from a group containing: A) the replacement of the glutamic acid series at position 560 in the collation: 2. With lysine series B) replacement of phenylalanine series at position 566 in arrangement: 2 with valine series C) replacement of glutamic acid series, methic acid series. Thionine, tyrosine series, isoleucine series, and alanine series at positions 22,236,461, 554, and 786, in collation: 2 with glycine series, varicose series. Lean, cysteine series, serine series, and valine series, respectively, and D) their combination 1 0. Any one of the claims. Items 1 to 9, where the bacteria belong to The genus was selected from a group consisting of Escherichia, Enetrrobacter, Erwinia, Klebsiella, Pantoea, Providencia, Salmonella, Serratia, Shigella, and Morganella 1. 1. Means according to any of the Claims 1 to 10, where such L-Amino is Choose from a group consisting of L-threonine, L-lysine, L-histidine, L-phenylalanine, L-arginine, L-. Tryptophan, L-glutamic acid, and L-lucene L-glutamic