RU2007106185A - BACTERIA OF THE FAMILY ENTEPOBACTERIACEAE, WHICH EXPRESSION OF THE OPERON YcbPONME (SSUEADCB) WAS WEARED, IS A PRODUCER OF L-AMINO ACID AND A METHOD FOR PRODUCING L-AMINO ACID - Google Patents

BACTERIA OF THE FAMILY ENTEPOBACTERIACEAE, WHICH EXPRESSION OF THE OPERON YcbPONME (SSUEADCB) WAS WEARED, IS A PRODUCER OF L-AMINO ACID AND A METHOD FOR PRODUCING L-AMINO ACID Download PDF

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Publication number
RU2007106185A
RU2007106185A RU2007106185/13A RU2007106185A RU2007106185A RU 2007106185 A RU2007106185 A RU 2007106185A RU 2007106185/13 A RU2007106185/13 A RU 2007106185/13A RU 2007106185 A RU2007106185 A RU 2007106185A RU 2007106185 A RU2007106185 A RU 2007106185A
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RU
Russia
Prior art keywords
amino acid
aromatic
group
ycbponme
operon
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RU2007106185/13A
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Russian (ru)
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RU2392326C2 (en
Inventor
Дмитрий Владимирович Филипов (RU)
Дмитрий Владимирович Филипов
Эльвира Борисовна Ворошилова (RU)
Эльвира Борисовна Ворошилова
тинер Михаил Маркович Гус (RU)
Михаил Маркович Гусятинер
Original Assignee
Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" (ЗАО АГРИ) (RU)
Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" (ЗАО АГРИ)
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Application filed by Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" (ЗАО АГРИ) (RU), Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" (ЗАО АГРИ) filed Critical Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" (ЗАО АГРИ) (RU)
Priority to RU2007106185/13A priority Critical patent/RU2392326C2/en
Priority to PCT/JP2008/052789 priority patent/WO2008105276A1/en
Publication of RU2007106185A publication Critical patent/RU2007106185A/en
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Publication of RU2392326C2 publication Critical patent/RU2392326C2/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids

Claims (11)

1. Бактерия-продуцент L-аминокислоты, принадлежащая к семейству Enterobacteriaceae, модифицированная таким образом, что экспрессия оперона ycbPONME в указанной бактерии ослаблена.1. The bacterium producing L-amino acids belonging to the Enterobacteriaceae family, modified so that the expression of the ycbPONME operon in the specified bacteria is weakened. 2. Бактерия по п.1, отличающаяся тем, что экспрессия указанного оперона ycbPONME ослаблена за счет инактивации указанного оперона ycbPONME.2. The bacterium according to claim 1, characterized in that the expression of the specified ycbPONME operon is weakened due to the inactivation of the specified ycbPONME operon. 3. Бактерия по п.1, отличающаяся тем, что указанная бактерия принадлежит к роду Escherichia.3. The bacterium according to claim 1, characterized in that said bacterium belongs to the genus Escherichia. 4. Бактерия по п.1, отличающаяся тем, что указанная бактерия принадлежит к роду Pantoea.4. The bacterium according to claim 1, characterized in that said bacterium belongs to the genus Pantoea. 5. Бактерия-продуцент L-аминокислоты по п.1, отличающаяся тем, что указанная L-аминокислота выбрана из группы, состоящей из ароматической L-аминокислоты и неароматической L-аминокислоты.5. The L-amino acid producing bacterium according to claim 1, characterized in that said L-amino acid is selected from the group consisting of an aromatic L-amino acid and a non-aromatic L-amino acid. 6. Бактерия-продуцент L-аминокислоты по п.5, отличающаяся тем, что указанная ароматическая L-аминокислота выбрана из группы, состоящей из L-фенилаланина, L-тирозина и L-триптофана.6. The L-amino acid producing bacterium according to claim 5, characterized in that said aromatic L-amino acid is selected from the group consisting of L-phenylalanine, L-tyrosine and L-tryptophan. 7. Бактерия-продуцент L-аминокислоты по п.5, отличающаяся тем, что указанная неароматическая L-аминокислота выбрана из группы, состоящей из L-треонина, L-лизина, L-цистеина, L-метионина, L-лейцина, L-изолейцина, L-валина, L-гистидина, L-глицина, L-серина, L-аланина, L-аспарагина, L-аспартата, L-глутамина, L-глутаминовой кислоты, L-пролина и L-аргинина.7. The L-amino acid producing bacterium according to claim 5, characterized in that said non-aromatic L-amino acid is selected from the group consisting of L-threonine, L-lysine, L-cysteine, L-methionine, L-leucine, L- isoleucine, L-valine, L-histidine, L-glycine, L-serine, L-alanine, L-asparagine, L-aspartate, L-glutamine, L-glutamic acid, L-proline and L-arginine. 8. Способ получения L-аминокислоты, включающий8. The method of obtaining L-amino acids, including выращивание бактерии по любому из пп.1-7 в питательной среде, вызывающее продукцию и накопление указанной L-аминокислоты в культуральной жидкости; иgrowing bacteria according to any one of claims 1 to 7 in a nutrient medium, causing production and accumulation of the specified L-amino acids in the culture fluid; and выделение указанной L-аминокислоты из культуральной жидкости.the allocation of the specified L-amino acids from the culture fluid. 9. Способ по п.8, отличающийся тем, что указанная L-аминокислота выбрана из группы, состоящей из ароматической L-аминокислоты и неароматической L-аминокислоты.9. The method of claim 8, wherein said L-amino acid is selected from the group consisting of an aromatic L-amino acid and a non-aromatic L-amino acid. 10. Способ по п.9, отличающийся тем, что указанная ароматическая L-аминокислота выбрана из группы, состоящей из L-фенилаланина, L-тирозина и L-триптофана.10. The method according to claim 9, characterized in that said aromatic L-amino acid is selected from the group consisting of L-phenylalanine, L-tyrosine and L-tryptophan. 11. Способ по п.9, отличающийся тем, что указанная неароматическая L-аминокислота выбрана из группы, состоящей из L-треонина, L-лизина, L-цистеина, L-метионина, L-лейцина, L-изолейцина, L-валина, L-гистидина, L-глицина, L-серина, L-аланина, L-аспарагина, L-аспартата, L-глутамина, L-глутаминовой кислоты, L-пролина и L-аргинина.11. The method according to claim 9, characterized in that said non-aromatic L-amino acid is selected from the group consisting of L-threonine, L-lysine, L-cysteine, L-methionine, L-leucine, L-isoleucine, L-valine , L-histidine, L-glycine, L-serine, L-alanine, L-asparagine, L-aspartate, L-glutamine, L-glutamic acid, L-proline and L-arginine.
RU2007106185/13A 2007-02-20 2007-02-20 METHOD OF L-TREONIN MANUFACTURE USING BACTERIA OF Escherichia GENUS WITH INACTIVATED OPERON ycbPONME OPERON (ssuEADCB) RU2392326C2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
RU2007106185/13A RU2392326C2 (en) 2007-02-20 2007-02-20 METHOD OF L-TREONIN MANUFACTURE USING BACTERIA OF Escherichia GENUS WITH INACTIVATED OPERON ycbPONME OPERON (ssuEADCB)
PCT/JP2008/052789 WO2008105276A1 (en) 2007-02-20 2008-02-13 A METHOD FOR PRODUCING AN L-AMINO ACID USING A BACTERIUM OF THE ENTEROBACTERIACEAE FAMILY WITH ATTENUATED EXPRESSION OF THE ycbPONME OPERON (ssuEADCB OPERON)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
RU2007106185/13A RU2392326C2 (en) 2007-02-20 2007-02-20 METHOD OF L-TREONIN MANUFACTURE USING BACTERIA OF Escherichia GENUS WITH INACTIVATED OPERON ycbPONME OPERON (ssuEADCB)

Publications (2)

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RU2007106185A true RU2007106185A (en) 2008-08-27
RU2392326C2 RU2392326C2 (en) 2010-06-20

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RU2007106185/13A RU2392326C2 (en) 2007-02-20 2007-02-20 METHOD OF L-TREONIN MANUFACTURE USING BACTERIA OF Escherichia GENUS WITH INACTIVATED OPERON ycbPONME OPERON (ssuEADCB)

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RU (1) RU2392326C2 (en)
WO (1) WO2008105276A1 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113136395A (en) * 2020-01-16 2021-07-20 南京理工大学 Expression vector of membrane protein YcbM and expression and purification method thereof

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WO2008105276A1 (en) 2008-09-04
RU2392326C2 (en) 2010-06-20

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