RU2392326C2 - METHOD OF L-TREONIN MANUFACTURE USING BACTERIA OF Escherichia GENUS WITH INACTIVATED OPERON ycbPONME OPERON (ssuEADCB) - Google Patents

METHOD OF L-TREONIN MANUFACTURE USING BACTERIA OF Escherichia GENUS WITH INACTIVATED OPERON ycbPONME OPERON (ssuEADCB) Download PDF

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RU2392326C2
RU2392326C2 RU2007106185/13A RU2007106185A RU2392326C2 RU 2392326 C2 RU2392326 C2 RU 2392326C2 RU 2007106185/13 A RU2007106185/13 A RU 2007106185/13A RU 2007106185 A RU2007106185 A RU 2007106185A RU 2392326 C2 RU2392326 C2 RU 2392326C2
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operon
ycbponme
inactivated
manufacture
bacteria
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RU2007106185/13A
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RU2007106185A (en
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Дмитрий Владимирович Филиппов (RU)
Дмитрий Владимирович Филиппов
Эльвира Борисовна Ворошилова (RU)
Эльвира Борисовна Ворошилова
Михаил Маркович ГУСЯТИНЕР (RU)
Михаил Маркович Гусятинер
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Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" (ЗАО АГРИ)
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Priority to RU2007106185/13A priority Critical patent/RU2392326C2/en
Priority to PCT/JP2008/052789 priority patent/WO2008105276A1/en
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids

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  • Life Sciences & Earth Sciences (AREA)
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  • Organic Chemistry (AREA)
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  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
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  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • General Chemical & Material Sciences (AREA)
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Abstract

FIELD: medicine.
SUBSTANCE: invention is method of L-threonine manufacture using bacteria of Escherichia genus modified in the way to inactivate operon ycbPONME.
EFFECT: highly effective manufacture of L-threonine.
3 cl, 2 dwg, 2 tbl, 11 ex

Description

Текст описания приведен в факсимильном виде.

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The text of the description is given in facsimile form.
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Claims (3)

1. Бактерия, принадлежащая к роду Escherichia, - продуцент L-треонина, модифицированная таким образом, что в указанной бактерии инактивирован оперон ycbPONME.1. A bacterium belonging to the genus Escherichia is a producer of L-threonine, modified in such a way that the ycbPONME operon is inactivated in this bacterium. 2. Бактерия по п.1, отличающаяся тем, что указанный оперон ycbPONME инактивирован за счет делеции оперона ycbPONME в хромосоме бактерии.2. The bacterium according to claim 1, characterized in that said ycbPONME operon is inactivated due to deletion of the ycbPONME operon in the bacterial chromosome. 3. Способ получения L-треонина, включающий:
выращивание бактерии по любому из пп.1 и 2 в питательной среде, вызывающее продукцию и накопление L-треонина в культуральной жидкости; и
выделение L-треонина из культуральной жидкости. 3. A method of obtaining L-threonine, including:
growing bacteria according to any one of claims 1 and 2 in a nutrient medium, causing production and accumulation of L-threonine in the culture fluid; and
isolation of L-threonine from the culture fluid.
RU2007106185/13A 2007-02-20 2007-02-20 METHOD OF L-TREONIN MANUFACTURE USING BACTERIA OF Escherichia GENUS WITH INACTIVATED OPERON ycbPONME OPERON (ssuEADCB) RU2392326C2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
RU2007106185/13A RU2392326C2 (en) 2007-02-20 2007-02-20 METHOD OF L-TREONIN MANUFACTURE USING BACTERIA OF Escherichia GENUS WITH INACTIVATED OPERON ycbPONME OPERON (ssuEADCB)
PCT/JP2008/052789 WO2008105276A1 (en) 2007-02-20 2008-02-13 A METHOD FOR PRODUCING AN L-AMINO ACID USING A BACTERIUM OF THE ENTEROBACTERIACEAE FAMILY WITH ATTENUATED EXPRESSION OF THE ycbPONME OPERON (ssuEADCB OPERON)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
RU2007106185/13A RU2392326C2 (en) 2007-02-20 2007-02-20 METHOD OF L-TREONIN MANUFACTURE USING BACTERIA OF Escherichia GENUS WITH INACTIVATED OPERON ycbPONME OPERON (ssuEADCB)

Publications (2)

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RU2007106185A RU2007106185A (en) 2008-08-27
RU2392326C2 true RU2392326C2 (en) 2010-06-20

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RU2007106185/13A RU2392326C2 (en) 2007-02-20 2007-02-20 METHOD OF L-TREONIN MANUFACTURE USING BACTERIA OF Escherichia GENUS WITH INACTIVATED OPERON ycbPONME OPERON (ssuEADCB)

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RU (1) RU2392326C2 (en)
WO (1) WO2008105276A1 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113136395A (en) * 2020-01-16 2021-07-20 南京理工大学 Expression vector of membrane protein YcbM and expression and purification method thereof

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
VAN DER PLOEG JR et. al. The Escherichia coli ssuEADCB gene cluster is required for the utilization of sulfur from aliphatic sulfonates and is regulated by the transcriptional activator Cbl. J. Biol. Chem. 1999 Oct 8; 274(41):29358-65. *

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RU2007106185A (en) 2008-08-27

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