RU2337960C2 - METHOD OF OBTAINING L-THREONINE AND L-ARGININE USING BACTERIUM, BELONGING TO GENUS Escherichia, IN WHICH GENE CLUSTER yehABCDE IS INACTIVATED - Google Patents

METHOD OF OBTAINING L-THREONINE AND L-ARGININE USING BACTERIUM, BELONGING TO GENUS Escherichia, IN WHICH GENE CLUSTER yehABCDE IS INACTIVATED Download PDF

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RU2337960C2
RU2337960C2 RU2006118968/13A RU2006118968A RU2337960C2 RU 2337960 C2 RU2337960 C2 RU 2337960C2 RU 2006118968/13 A RU2006118968/13 A RU 2006118968/13A RU 2006118968 A RU2006118968 A RU 2006118968A RU 2337960 C2 RU2337960 C2 RU 2337960C2
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RU
Russia
Prior art keywords
threonine
arginine
yehabcde
bacterium
gene cluster
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RU2006118968/13A
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Russian (ru)
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RU2006118968A (en
Inventor
Дмитрий Владимирович Филиппов (RU)
Дмитрий Владимирович Филиппов
Эльвира Борисовна Ворошилова (RU)
Эльвира Борисовна Ворошилова
тинер Михаил Маркович Гус (RU)
Михаил Маркович Гусятинер
Original Assignee
Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" (ЗАО АГРИ)
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Priority to RU2006118968/13A priority Critical patent/RU2337960C2/en
Priority to PCT/JP2007/061238 priority patent/WO2007139220A1/en
Publication of RU2006118968A publication Critical patent/RU2006118968A/en
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Abstract

FIELD: chemistry, biotechnology.
SUBSTANCE: invention relates to biotechnology and represents method of obtaining L-threonine or L-arginine using bacterium belonging to genus Escherichia, modified in such way that in said bacterium gene cluster yehABCDE is inactivated.
EFFECT: obtaining L-threonine or L-arginine with high degree of efficiency.
3 cl, 2 dwg, 3 tbl, 11 ex

Description

Текст описания приведен в факсимильном виде.

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The text of the description is given in facsimile form.
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Claims (3)

1. Бактерия, принадлежащая к роду Escherichia, - продуцент L-треонина или L-аргинина, модифицированная таким образом, что в указанной бактерии инактивирован кластер генов yehABCDE.1. A bacterium belonging to the genus Escherichia is a producer of L-threonine or L-arginine, modified in such a way that the yehABCDE gene cluster is inactivated in this bacterium. 2. Бактерия по п.1, отличающаяся тем, что указанный кластер генов yehABCDE инактивирован за счет делеции кластера генов yehABCDE в хромосоме бактерии.2. The bacterium according to claim 1, characterized in that said yehABCDE gene cluster is inactivated by deletion of the yehABCDE gene cluster in the bacterial chromosome. 3. Способ получения L-треонина или L-аргинина, включающий выращивание бактерии по любому из пп.1 и 2 в питательной среде, вызывающее продукцию и накопление указанной L-аминокислоты в культуральной жидкости; и выделение указанной L-аминокислоты из культуральной жидкости.3. A method for producing L-threonine or L-arginine, comprising growing a bacterium according to any one of claims 1 and 2 in a nutrient medium, causing production and accumulation of said L-amino acid in the culture fluid; and isolating said L-amino acid from the culture fluid.
RU2006118968/13A 2006-06-01 2006-06-01 METHOD OF OBTAINING L-THREONINE AND L-ARGININE USING BACTERIUM, BELONGING TO GENUS Escherichia, IN WHICH GENE CLUSTER yehABCDE IS INACTIVATED RU2337960C2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
RU2006118968/13A RU2337960C2 (en) 2006-06-01 2006-06-01 METHOD OF OBTAINING L-THREONINE AND L-ARGININE USING BACTERIUM, BELONGING TO GENUS Escherichia, IN WHICH GENE CLUSTER yehABCDE IS INACTIVATED
PCT/JP2007/061238 WO2007139220A1 (en) 2006-06-01 2007-05-28 A method for producing an l-amino acid using a bacterium of the enterobacteriaceae family with attenuated expression of the yehabcde cluster

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
RU2006118968/13A RU2337960C2 (en) 2006-06-01 2006-06-01 METHOD OF OBTAINING L-THREONINE AND L-ARGININE USING BACTERIUM, BELONGING TO GENUS Escherichia, IN WHICH GENE CLUSTER yehABCDE IS INACTIVATED

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RU2006118968A RU2006118968A (en) 2007-12-10
RU2337960C2 true RU2337960C2 (en) 2008-11-10

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Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
SERRES M.H. A functional update of the Escherichia coli K-12 genome. Genome Biol. 2001; 2(9): RESEARCH 0035. Epub. 2001, Aug. 20. *

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