RU2337960C2 - METHOD OF OBTAINING L-THREONINE AND L-ARGININE USING BACTERIUM, BELONGING TO GENUS Escherichia, IN WHICH GENE CLUSTER yehABCDE IS INACTIVATED
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METHOD OF OBTAINING L-THREONINE AND L-ARGININE USING BACTERIUM, BELONGING TO GENUS Escherichia, IN WHICH GENE CLUSTER yehABCDE IS INACTIVATED
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RU2337960C2
RU2337960C2RU2006118968/13ARU2006118968ARU2337960C2RU 2337960 C2RU2337960 C2RU 2337960C2RU 2006118968/13 ARU2006118968/13 ARU 2006118968/13ARU 2006118968 ARU2006118968 ARU 2006118968ARU 2337960 C2RU2337960 C2RU 2337960C2
Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" (ЗАО АГРИ)
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Priority to RU2006118968/13ApriorityCriticalpatent/RU2337960C2/en
Priority to PCT/JP2007/061238prioritypatent/WO2007139220A1/en
Publication of RU2006118968ApublicationCriticalpatent/RU2006118968A/en
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Publication of RU2337960C2publicationCriticalpatent/RU2337960C2/en
Preparation Of Compounds By Using Micro-Organisms
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Micro-Organisms Or Cultivation Processes Thereof
(AREA)
Abstract
FIELD: chemistry, biotechnology.
SUBSTANCE: invention relates to biotechnology and represents method of obtaining L-threonine or L-arginine using bacterium belonging to genus Escherichia, modified in such way that in said bacterium gene cluster yehABCDE is inactivated.
EFFECT: obtaining L-threonine or L-arginine with high degree of efficiency.
3 cl, 2 dwg, 3 tbl, 11 ex
Description
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The text of the description is given in facsimile form.
Claims (3)
1. Бактерия, принадлежащая к роду Escherichia, - продуцент L-треонина или L-аргинина, модифицированная таким образом, что в указанной бактерии инактивирован кластер генов yehABCDE.1. A bacterium belonging to the genus Escherichia is a producer of L-threonine or L-arginine, modified in such a way that the yehABCDE gene cluster is inactivated in this bacterium.2. Бактерия по п.1, отличающаяся тем, что указанный кластер генов yehABCDE инактивирован за счет делеции кластера генов yehABCDE в хромосоме бактерии.2. The bacterium according to claim 1, characterized in that said yehABCDE gene cluster is inactivated by deletion of the yehABCDE gene cluster in the bacterial chromosome.3. Способ получения L-треонина или L-аргинина, включающий выращивание бактерии по любому из пп.1 и 2 в питательной среде, вызывающее продукцию и накопление указанной L-аминокислоты в культуральной жидкости; и выделение указанной L-аминокислоты из культуральной жидкости.3. A method for producing L-threonine or L-arginine, comprising growing a bacterium according to any one of claims 1 and 2 in a nutrient medium, causing production and accumulation of said L-amino acid in the culture fluid; and isolating said L-amino acid from the culture fluid.
RU2006118968/13A2006-06-012006-06-01METHOD OF OBTAINING L-THREONINE AND L-ARGININE USING BACTERIUM, BELONGING TO GENUS Escherichia, IN WHICH GENE CLUSTER yehABCDE IS INACTIVATED
RU2337960C2
(en)
Method of obtaining l-threonine with use of bacterium belonging to sort escherichia, modified in such manner that ability to form "kerly" type villus is broken
METHOD FOR PRODUCING L-AMINO ACID USING BACTERIA OF THE Enterobacteriaceae FAMILY IN WHICH EXPRESSION OF THE ydiN GENE, ydiB GENE OR THEIR COMBINATION IS DECREASED
BACTERIA OF THE GENUS Escherichia Possessing Enhanced Expression of the ACEEF-lpdA GENES OPERON AND METHOD FOR PRODUCING SUITIC ACID USING SUCH BACTERIA