TH23481Y - A method for producing fish oil from fish processing waste materials through enzymatic hydrolysis combined with mechanical separation. - Google Patents

Abstract

The process for producing fish oil from fish processing waste materials through enzymatic digestion combined with mechanical separation, as described in this invention, consists of raw material preparation steps, including reducing the size of bone fragments with attached flesh to a suitable level for protein digestion and improved oil separation; protein digestion from muscle using protease enzymes; centrifugal emulsion separation; and oil separation by protein precipitation with salt, to obtain clear fish oil free from sediment or impurities.

Claims (10)

1. The process of producing fish oil from fish processing waste materials through enzymatic hydrolysis combined with mechanical separation involves the following steps: Step A: Raw Material Preparation. Fish waste materials, primarily fish bones, are reduced in size to small pieces, resulting in small fish skeletons. Step B: Muscle Protein Hydrolysis. The small fish skeletons from Step A are mixed with water, the pH of the mixture is adjusted to 7.5-8.0, and the temperature is set to 50-65 degrees Celsius. Then, 1-3% of the weight of the fish skeleton (weight-to-weight) of protease enzyme is added, and the mixture is continuously stirred for 1-3 hours. Afterwards, the solid bone components are separated out. This step yields a protein solution. Step C: Emulsion Separation. The protein solution from Step B is then emulsified to separate the emulsion from the protein solution, which primarily consists of water and protein. A highly concentrated oil emulsion will be obtained. Step D: Oil Separation. Add a saturated salt solution to the highly concentrated oil emulsion from step C while continuously stirring for 0.5-2 hours. After the time is up, centrifuge at 5,000-20,000 rpm for 10-60 minutes at a temperature of 24-26 degrees Celsius to separate the oil from the sediment. Remove any dispersed water in the oil by adding salt powder. Finally, filter to separate the oil. This yields fish oil from fish processing waste materials through enzymatic hydrolysis combined with mechanical separation. 2. A method for producing fish oil from fish processing waste materials through enzymatic digestion combined with mechanical separation, as per claim 1, where step b, muscle protein digestion, the optimal ratio is fish carcass to water equal to 1:2-1:5 (weight to volume). 3. The process for producing fish oil from fish processing waste materials through enzymatic hydrolysis combined with mechanical separation, as per claim 1, where the optimal step (step b) for muscle protein hydrolysis is to adjust the pH with a sodium hydroxide or potassium hydroxide solution. 4. The process for producing fish oil from fish processing waste materials through enzymatic digestion combined with mechanical separation, as per claim 1, where the appropriate steps are: muscle protein digestion and separation of solid bone from the protein solution, which involves filtration with cheesecloth or a sieve. 5. The process for producing fish oil from fish processing waste materials through enzymatic hydrolysis combined with mechanical separation, as per Patent 1, where the appropriate emulsion separation step (step C) is via a rotary centrifuge with an outlet pressure of 20-40 psi on the protein solution side and 5-20 psi on the emulsion side, repeated 1-3 times, 2-5 times. 6. A method for producing fish oil from fish processing waste materials through enzymatic digestion combined with mechanical separation, as per claim 1, where step d, oil separation, yields a saturated salt solution selectable from ammonium sulfate, sodium sulfate, magnesium sulfate, potassium sulfate, or sodium chloride. 7. A method for producing fish oil from fish processing waste materials through enzymatic digestion combined with mechanical separation, as per claim 1 or 6, where the oil separation step, the appropriate saturated salt solution is saturated ammonium sulfate. 8. A method for producing fish oil from fish processing waste materials through enzymatic hydrolysis combined with mechanical separation, as per Patent 1, where step d involves oil separation using an optimal emulsion-to-salt solution ratio of 50:1-10:1 (weight to volume). 9. A method for producing fish oil from fish processing waste materials through enzymatic digestion combined with mechanical separation, as per claim 1, where in step 2, oil separation, the salt powder can be selected from sodium sulfate or ammonium sulfate. 10. A method for producing fish oil from fish processing waste materials through enzymatic digestion combined with mechanical separation, as per claim 1 or 9, where the appropriate oil separation step is sodium sulfate powder at a concentration of 2-10% (weight/weight).