TH112255A - The digestion and fermentation of cassava in the same process to produce ethanol. - Google Patents

Abstract

DC60 This invention discloses a process for digesting and fermenting cassava chips in one step to produce ethanol, comprising the following steps: 1. The cassava chips must be ground to a size suitable for the fermentation process, i.e., through a milling machine with a sieve hole size of 2 mm to produce a flour powder from the cassava chips with a fineness of 10 mesh, and the cassava chips must contain a starch content of more than 70 percent by dry weight and the sand content must be less than 2 percent by dry weight. 2. The cassava chips must be first digested with alpha-amylase. (Alpha-amylase) Commercial rent Termamyl Registered Trademark 120L (0.1 percent by dry weight) at pH 5.5 - 6.0, add salt, calcium chloride (CaCI2.2H2O) (0.1 percent by dry weight) to accelerate the digestion reaction better. Increase the temperature in the digester to 95-100 degrees Celsius, digest for 2 hours, then stop the digestion, then reduce the temperature to approximately 60-65 degrees Celsius. 3. Must digest a second time and ferment in the same step by adding commercial glucoamylase enzyme such as AMG Registered Trademark 300L (0.1 percent by dry weight) working at temperature 60-65 degrees Celsius, pH 4.0 - 4.5 for 2 hours, then reduce the temperature to 30-35 degrees Celsius, must add yeast. Saccharomyces cerevisiae immediately and ferment for 48 hours. 4. Then filter the juice through a filter and purify the ethanol by distillation. The residue from the filtration is passed through a pulp press to dry it and be used as animal feed. This invention discloses a process for digesting and fermenting cassava chips in the same step to produce ethanol, consisting of the following steps: 1. The cassava chips must be ground to a size suitable for the fermentation process, i.e., through a milling machine with a sieve hole size of 2 mm to obtain a flour powder from cassava chips with a fineness of 10 mesh. The cassava chips must have a starch content of more than 70 percent by dry weight and the sand content must be less than 2 percent by dry weight. 2. The cassava chips must be first digested with alpha-amylase. (Alpha-amylase) Commercial rental Termamyl R 120L (0.1% by dry weight) at pH 5.5 - 6.0, add calcium chloride (CaCI2.2H2O) (0.1% by dry weight) to accelerate the digestion reaction. Increase the temperature in the digester to 95-100 degrees Celsius, digest for 2 hours, then stop the digestion, then reduce the temperature to approximately 60-65 degrees Celsius. 3. Must be digested a second time and fermented in the same step by adding commercial glucoamylase enzyme such as AMG R 300L (0.1% by dry weight) working at a temperature of 60-65 degrees Celsius, pH 4.0 - 4.5 for 2 hours, then reduce the temperature to 30-35 degrees Celsius, must add yeast. Saccharomyces cerevisiae is immediately fermented for 48 hours. 4. Then filter the molasses through a filter and purify the ethanol by distillation. The residue left from the filtration is pressed through a pulp press to dry it and used as animal feed.

Claims (1)

: DC60 This invention discloses the digestion and fermentation process in the same process to produce ethanol. It consists of the following steps: 1. The cassava must be crushed to a suitable size for the fermentation process, ie through a milling machine with Hole size Sieve equal to 2 mm to make cassava powder with a fineness of 10 mesh and must use cassava with more than 70% starch content by dry weight. The sand content must be less than 2% by dry weight. 2. The first cassava must be digested with protease-alpha-amylase. (Alpha-amylase) commercial lease Termamyl Registered Trademark 120L (0.1% dry weight) at the pH conditions of 5.5 - 6.0, add calcium chloride salt (CaCI2.2H2O) (% 0.1 by dry weight) is used to speed up the digestion reaction, increase the temperature in the digestion tank to 95-100 ° C, digest for 2 hours, stop digestion, then lower the temperature to approximately 60-65. Degrees Celsius 3. It needs a second digestion and fermentation at the same stage. By adding Glucoamylase protease (Glucoamylase), for example, AMG Registered Trademark 300L (0.1% by dry weight), worked at temperature 60-65 ° C, pH 4.0 - 4.5 for 2 hours, then reduced temperature to 30-35 degrees. Celsius, yeast must be added. Saccharomyces cerevisiae was immediately fermented for 48 hours. 4. Then the water was filtered through a strainer and purified by ethanol by distillation. The residue from the filtration is passed through a pulverizer. To dry Lead to animal feed This invention discloses the same digestion and fermentation process to produce ethanol. It consists of the following steps: 1. Cassava must be crushed to a suitable size for the fermentation process, through a milling machine with a hole size. Sieve equal to 2 mm to make cassava powder with a fineness of 10 mesh and must use cassava with more than 70% starch content by dry weight. The sand content must be less than 2% by dry weight. 2. The first cassava must be digested with protease-alpha-amylase. (Alpha-amylase) commercial lease Termamyl R 120L (0.1% dry weight) at the pH conditions of 5.5 - 6.0 add calcium chloride salt (CaCI2.2H2O) (0.1% By dry weight) used to speed up the digestion reaction, increase the temperature in the digestion tank to 95-100 ° C, digest for 2 hours, stop digestion, then lower the temperature to about 60-65 degrees. C. 3. Requires second digestion and fermentation at the same stage. By adding Glucoamylase protease (Glucoamylase), for example, AMG R 300L (0.1% by dry weight), worked at the temperature of 60-65 degrees Celsius, pH 4.0 - 4.5 for 2 hours, then reduced the temperature to 30-35 degrees Celsius. Yeast must be added. Saccharomyces cerevisiae was immediately fermented for 48 hours. 1. This invention revealed the same digestion and fermentation process to produce ethanol. It consists of the following steps: a. Must grind cassava to a suitable size for the fermentation process, that is, through a size mill. The sieve hole is 2 mm in order to obtain cassava powder with a resolution of 10 mesh and must use cassava with more than 70% starch content by dry weight and volume. The sand should be less than 2% by dry weight. B.