CN107083411A - A kind of preprocess method of wheat straw waste enzymolysis - Google Patents
A kind of preprocess method of wheat straw waste enzymolysis Download PDFInfo
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- CN107083411A CN107083411A CN201710076437.7A CN201710076437A CN107083411A CN 107083411 A CN107083411 A CN 107083411A CN 201710076437 A CN201710076437 A CN 201710076437A CN 107083411 A CN107083411 A CN 107083411A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/02—Monosaccharides
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P2201/00—Pretreatment of cellulosic or lignocellulosic material for subsequent enzymatic treatment or hydrolysis
Abstract
The present invention relates to a kind of preprocess method for realizing wheat straw waste hydrolysis, step is as follows：After wheat straw waste is soaked, hydrogen peroxide, sodium hydroxide are added, then distilled water carry out boiling, extruded with extruder, carrying out two sections of grinding processing, screening, enzyme hydrolysis, enzyme liquid is added under aseptic condition to be digested under constant temperature again, and the hydrolyzate of wheat straw waste is produced after enzymolysis.The inventive method takes full advantage of some existing equipment in slurrying industry, reduces additional investment, saves substantial amounts of financial cost, and the recycling of existing paper industry recycling is of great importance, is conducive to the sustainable development of pulp and paper industry.
Description
Technical field
The invention belongs to technical fields such as the recyclings after wheat straw waste hydrolysis, there is provided a kind of efficient pre- place of hydrolysis
Reason technology.
Background technology
Biomass is organic energy most abundant on the earth, is a kind of inexhaustible, nexhaustible regenerative resource, is
Coal, the fourth-largest energy of oil and natural gas resource are only second on the earth.Biomass is that the most important energy of human society comes
Source, annual yield reaches 170,000,000,000 tons, and these abundant biomass resources are to realizing the sustainable development of World Economics, realizing
Effective utilize of environmental protection and resource has great importance.At present, utilization major embodiment both at home and abroad to biomass resource
In terms of alcohol fuel, biodiesel, biomass electric energy, biogas, biological material.
Pulp and paper industry is the industry of earliest utilization native cellulose, is also that only one collection is gathered, added on a large scale
Work and the industry using biomass material.Traditional pulp and paper industry is combined with modern biorefinery, in raw material
Hemicellulose and lignin be converted into new biomass product, traditional pulp mill is become a modern pulp bio matter essence
Joint processing factory is refined, accomplishes the separation of hemicellulose, lignin, cellulose and volatility extract, in addition to producing paper pulp,
The components of biomass such as hemicellulose and lignin is extracted from waste liquid or pretreatment fluid, high added value is further produced by conversion
Product, such as ethanol, carbon fiber, polymer and biodiesel, it is possible to expand industrial chain significantly, make the utilization of biomass resource
And maximizing the benefits, the level of profitability of paper industry is improved, therefore, biorefinery provides for the recovery and development of paper industry
New opportunity and direction.Pulping and paper-making is combined with biomass energy, while papermaking enterprise obtains paper pulp and paper, may be used also
To produce the fuels and chemicals of high added value, such as ethanol, carbon fiber, polymer, kerosene and biodiesel, this is also beneficial to reality
The sustainable development of existing pulp and paper industry.
A kind of straw biological enzyme prodegradant of CN102230288A technical field of straw degradation, including cellulase 8-10%,
Lipase 6-11%, α -1,4- glucose hydrolysis enzymes 6-10%, catalase 4-10%, urea 6-20%, magnesium sulfate 6-16%, fat
Fat alcohol APEO 8-15%, APES OP-1011-15%, surplus is excipient.Stalk is cleaned into segment,
Put into fast pulping machine, add water and straw biological enzyme prodegradant, be well mixed, 50- is heated in fast pulping machine
60 DEG C are rubbed with the hands mill, reaction 1-1.5 hours；Then hydrogen peroxide bleaching is added, when whiteness is more than 70%ISO, terminating reaction obtains paper pulp.This
Invention is without thermophilic digestion, without highly basic strong acid, pollution-free, meets the requirement of environment-friendly pulping, slurrying non-wastewater discharge, and can
A large amount of industrial waters are saved, the energy is saved, production cost is reduced, accelerate repulping rate.Although the patent can be realized sufficiently
Non-pollution discharge, meets the requirement of environment-friendly pulping, but can not improve the multiple uses of stalk, realizes the repetition of biomass
Utilize.
The content of the invention
It is an object of the invention to overcome in place of the deficiencies in the prior art effectively to realize wheat straw waste enzyme water there is provided one kind
The preprocess method of solution.
A kind of preprocess method that achievable wheat straw waste is efficiently digested, step is as follows：
(1) wheat straw waste is placed in 90-120 DEG C or so of water and soaked 30-60 minutes, then extrude the stalk soaked
Machine is extruded, and removes the moisture in stalk；H is sequentially added into the wheat straw waste after extruding2O2, NaOH, distilled water, so
After carry out boiling, temperature control is at 90-120 DEG C, and digestion time is 1-3h.
(2) step will be passed through（1）Pretreated wheat straw waste is placed in extruder to be extruded again, after extrusion process
Wheat straw waste, to neutrality, then carries out extrusion dehydration, the wheat straw waste after being handled again by water washing.
(3) by NaOH and Na2SO3Mixed liquor is added in the wheat straw waste after treatment described in step (2), then
Handled again under constant temperature, treatment temperature is 90 DEG C -120 DEG C, and processing time 1-2h obtains the wheat straw after alkali process.
(4) wheat straw after handling in step (3) is carried out discongesting processing：Pressure grinding, normal pressure grinding are sequentially passed through, is obtained
Discongest rear wheat straw slurry.
(5) wheat straw slurry after grinding in step (4) is separated by screening and collecting by the sieve of 80-100 mesh,
It is 15%-25% that the wheat straw slurry recycling being collected into, which is dehydrated to concentration, is then 40 by defibrination to beating degree oSR -60oSR；The wheat straw slurry after mashing is collected, for follow-up enzymolysis.
(6) digest：
Acetic acid one sodium acetate buffers of the pH in 4-5, the quality of the gentle fliud flushing of wheat straw slurry are added after to mashing in wheat straw slurry
Volume ratio is g:ML is 1:15-1:20, added after being sufficiently stirred for and incubated enzymolysis is carried out under enzyme liquid, aseptic condition, cultivated
40 DEG C -50 DEG C of reaction temperature, reaction time 36-40h, pH=5-6 or so；Rotating speed 100-120 rpm.
It is preferred that, step（1）In, stalk is screened first, stalk of the screening length in 5-10cm or so.
It is preferred that, step（1）Described in stalk is cut into section, any instrument can be used to cut stalk to 1 ~ 10cm's
Section.The length of the stalk section is not limited, and those skilled in the art can be selected as needed.
It is preferred that, step（1）Described in add H into the wheat straw waste after extruding2O2For to the wheat after extruding
The H relative to over dry wheat straw waste quality 10%-20% is added in careless stalk2O2, the H2O2Mass percent concentration be 30%.
It is preferred that, step（1）Described in be directly added into NaOH into the wheat straw waste after extruding and be, add relative to
Over dry wheat straw quality 10%-30% NaOH.
It is preferred that, step（1）Described in into the wheat straw waste after extruding add distilled water be, over dry wheat straw waste
Quality is 1 with the mass volume ratio for adding the liquid after distilled water:5-1:10,（This absolute dry mass for referring to stalk and distillation
The volume ratio of water）.
It is preferred that, collect the liquid squeezed out after being extruded in step (2)；The liquid of collection contains lignin, hemicellulose
The materials such as element can carry out follow-up recycling.
It is preferred that, the neutrality described in step (2), it is 6.5 ~ 7.5 to refer to pH.
It is preferred that, NaOH and Na described in step (3)2SO3Mixed liquor, by relative to over dry wheat straw quality 5.0%-10%
NaOH and the Na relative to over dry wheat straw quality 5.0%-10%2SO3It is dissolved in distilled water and obtains.
It is preferred that, wheat straw waste mass concentration is 25%-35% in step (3).
It is preferred that, the pressure grinding described in step (4), grinding condition is 90 DEG C -120 DEG C of temperature, pressure 0.1-0.3
MPa, the mass concentration of wheat straw slurry is 25%-35%, grinding 5-10 minutes.
It is preferred that, the normal pressure grinding described in step (4), 90 DEG C -120 DEG C of temperature, pressure is between grinding under normal pressure, mill
Gap 0.10-0.3mm, the mass concentration 25%-35% of wheat straw slurry.
It is preferred that, the ratio of the gentle fliud flushing of wheat straw slurry described in step (6) is g:ML is 1:15-1:20, refer to every
Gram wheat straw slurry adds 15-20ml buffer solutions.
It is preferred that, step (6) is carried out under sterile conditions.
It is preferred that, the mass concentration of substrate is 5.0%-10% in step (6).
It is preferred that, enzyme liquid described in step (6) is cellulase and cellulose dextranase complex enzyme, cellulase
Consumption is 76 ~ 84CBU/g substrates, and the enzyme activity 35-45 IU/mL of cellulase used, temperature is 50 DEG C -60 DEG C, and pH value is 4.5-
6.0；Cellulose dextranase consumption is 31-33EGU/g substrates, and the enzyme activity of cellulose dextranase is 600-800EGU/g, temperature
Spend for 50-60 DEG C, pH value is 4.5-6.0.
Term is explained：
oSR：Also referred to as " beating degree ", represent manufactured paper with pulp on copper mesh paper when, the degree of slurry drainage speed, be weigh pulp quality
One of index.
Over dry wheat straw waste：Refer to wheat straw waste through drying, the weight of wheat straw waste when making moisture content be zero.
Fraction is dehydrated：The order of severity for referring to stalk dehydration is divided into Three Estate, is respectively：One-level is dehydrated；Two grades of dehydrations；Three
Level dehydration.
The advantages of the present invention are：
1st, the inventive method takes full advantage of existing equipment component in pulp industry, it is not necessary to carry out other complicated reaction bars
Part, saves the energy, reduces capital consumption, and can recycling wheat straw waste be conducive to the sustainable of paper industry
Development.
2nd, the present invention is combined by chemical method and grinding method and sufficiently cellulose can be extracted,
Most of lignin can also be separated from stalk.
3rd, the wheat straw waste of the inventive method is after enzymolysis 20 hours, and the concentration of glucose reaches 7560 mg/ in enzymolysis liquid
L, the inversion rate of glucose of substrate reaches 91.12%；After hydrolysis 40 hours, the concentration of glucose reaches 8020 mg/ in hydrolyzate
L, the inversion rate of glucose of substrate reaches 98.56%, and transformation efficiency is higher, and stalk must obtain sufficient recycling, favorably
In sustainable development.
Embodiment
Following examples formula is further illustrated to the present invention, but the invention is not limited in this.
Embodiment 1
(1) extension bar is screened first, stalk is cut into instrument 5cm section, wheat straw waste is then placed on 90 DEG C or so
Water in soak 30min, then the stalk soaked is extruded with extruder, remove stalk in moisture.To extruding
The H relative to over dry wheat straw waste quality 10% is added in wheat straw waste afterwards2O2, the H2O2Mass concentration be 30%, Ran Houjia
Enter the NaOH relative to over dry wheat straw quality 10%, then add distilled water, over dry wheat straw waste quality is with adding after distilled water
Total liquid volume ratio is 1:5, carry out boiling is then put into, temperature control is at 90 DEG C, and digestion time is 1h.
(2) H will be passed through2O2It is placed in extruder and extrudes again with the pretreated wheat straw wastes of NaOH, and collects extruding
Wheat straw waste after liquid out, extrusion process is fully washed by water, until then pH value carries out extruding de- again in neutrality
Water, the wheat straw waste after being handled.
(3) by relative to the NaOH of the over dry wheat straw quality 5.0% and Na relative to over dry wheat straw quality 5.0%2SO3Dissolving
In distilled water, then the mixed liquor is added in the wheat straw waste after treatment referred in step (2), add distillation
Water, it is 25% or so to make wheat straw waste mass concentration, is then handled again under constant temperature, and treatment temperature is 90 DEG C, during processing
Between 1h, the wheat straw after being handled.
(4) wheat straw after Chemical impregnation processing in step (3) is carried out discongesting processing.Pressure grinding, mill are carried out first
Solution condition is 90 DEG C of temperature, and the MPa of pressure 0.3, the mass concentration of wheat straw slurry is 25%, grinding 5 minutes；Then normal pressure mill is carried out
Solution, 90 DEG C of temperature, pressure is grinding under normal pressure, and disk mill gap 0.10mm, the mass concentration 25% of wheat straw slurry must discongest rear wheat
Straw pulp material.
(5) wheat straw pulp fiber after grinding in step (4) is separated by screening and collecting by the sieve of 80 mesh,
It is 15% that the wheat straw slurry recycling being collected into, which is dehydrated to concentration, is then 40 by defibrination to beating degree oSR, collect and beat
Wheat straw slurry after slurry, for follow-up enzymolysis.
(6) under sterile conditions, acetic acid one acetate buffers of the pH 5 or so is added into wheat straw slurry after mashing
Liquid, ratio is g:ML is 1:15, enzyme liquid is added after being sufficiently stirred for, then adding distilled water makes the mass concentration of substrate be 5.0%,
Incubated enzymolysis is carried out under aseptic condition, 40 DEG C of reaction temperature, reaction time 30h, pH=5 or so are cultivated；The rpm of rotating speed 100.
Wherein, enzyme liquid is cellulase and cellulose dextranase complex enzyme, and the consumption of cellulase is 80CBU/g substrates, fibre used
The IU/mL of enzyme activity 35 of the plain enzyme of dimension,；Cellulose dextranase consumption is 31EGU/g substrates, and the enzyme activity of cellulose dextranase is
600EGU/g, temperature is 50 DEG C, and pH value is 4.5.
It can thus be concluded that, under above-mentioned enzyme hydrolysis condition:After hydrolysis 15 hours, the concentration of glucose is in hydrolyzate
4330mg/L, the inversion rate of glucose of substrate is 67.42%;After hydrolysis 30 hours, the concentration of glucose is in hydrolyzate
5430mg/L, the inversion rate of glucose of substrate is 73.62%.
Embodiment 2
(1) extension bar is screened first, stalk is cut into instrument 5cm section, wheat straw waste is placed on 100 DEG C or so
Soak 45 minutes, then extruded the stalk soaked with extruder in water, remove the moisture in stalk.After extruding
Wheat straw waste in add the H relative to over dry wheat straw waste quality 15%2O2, the H2O2Mass concentration be 30%, then add
Relative to the NaOH of over dry wheat straw quality 20%, distilled water is then added, over dry wheat straw waste quality is total after distilled water with adding
Liquid volume compares 1:8, carry out boiling is then put into, temperature control is at 100 DEG C, and digestion time is 2h.
(2) H will be passed through2O2It is placed in extruder and extrudes again with the pretreated wheat straw wastes of NaOH, and collection is squeezed out
Wheat straw waste after the liquid come, extrusion process is fully washed by water, until then pH value carries out extruding de- again in neutrality
Water, the wheat straw waste after being handled.
(3) by relative to the NaOH of the over dry wheat straw quality 8% and Na relative to over dry wheat straw quality 8%2SO3It is dissolved in steaming
In distilled water, then the mixed liquor is added in the wheat straw waste after treatment referred in step (2), add distilled water, make
Wheat straw waste mass concentration is 30% or so, is then handled again under constant temperature, and treatment temperature is 100 DEG C, processing time
1.5h, the wheat straw after being handled.
(4) wheat straw after Chemical impregnation processing in step (3) is carried out discongesting processing.Pressure grinding, mill are carried out first
Solution condition is 100 DEG C of temperature, and the MPa of pressure 0.2, the mass concentration of wheat straw slurry is 30%, grinding 8 minutes；Then normal pressure is carried out
Grinding, 100 DEG C of temperature, pressure is grinding, disk mill gap 0.2mm, the mass concentration 30% of wheat straw slurry, after must discongesting under normal pressure
Wheat straw slurry.
(5) wheat straw pulp fiber after grinding in step (4) is separated by screening and collecting by the sieve of 90 mesh,
It is 20% that the wheat straw slurry recycling being collected into, which is dehydrated to concentration, is then 50 by defibrination to beating degreeoSR.Collection is beaten
Wheat straw slurry after slurry, for follow-up enzymolysis.
(6) under sterile conditions, acetic acid one acetate buffers of the pH 4.5 is added into wheat straw slurry after mashing
Liquid, ratio is g:ML is 1:20, enzyme liquid is added after being sufficiently stirred for, then adding distilled water makes the mass concentration of substrate be 8%, nothing
Incubated enzymolysis is carried out under the conditions of bacterium, 45 DEG C of reaction temperature, reaction time 38h, pH=5.5 or so are cultivated；The rpm of rotating speed 110.
Wherein, enzyme liquid is cellulase and cellulose dextranase complex enzyme, and the consumption of cellulase is 80CBU/g substrates, fibre used
The IU/mL of enzyme activity 40 of the plain enzyme of dimension, temperature is 55 DEG C, and pH value is 5.5；Cellulose dextranase consumption is 32EGU/g substrates, fine
The enzyme activity of the plain dextranase of dimension is 700EGU/g, and temperature is 55 DEG C, and pH value is 5.5.
It can thus be concluded that, under above-mentioned enzyme hydrolysis condition:After hydrolysis 20 hours, the concentration of glucose is in hydrolyzate
5580mg/L, the inversion rate of glucose of substrate is 79.25%;After hydrolysis 30 hours, the concentration of glucose is in hydrolyzate
6348mg/L, the inversion rate of glucose of substrate is 85.67%.
Embodiment 3
(1) extension bar is screened first, stalk is cut into instrument 5cm section, wheat straw waste is placed on 100 DEG C or so
Soak 60 minutes, then extruded the stalk soaked with extruder in water, remove the moisture in stalk.After extruding
Wheat straw waste in add the H relative to over dry wheat straw waste quality 20%2O2, the H2O2Mass concentration be 30%, then add
Relative to the NaOH of over dry wheat straw quality 30%, distilled water is then added, over dry wheat straw waste quality is total after distilled water with adding
Liquid volume ratio is 1:10, carry out boiling is then put into, temperature control is at 120 DEG C, and digestion time is 3h.
(2) H will be passed through2O2It is placed in extruder and extrudes again with the pretreated wheat straw wastes of NaOH, and collects extruding
Wheat straw waste after liquid out, extrusion process is fully washed by water, until then pH value carries out extruding de- again in neutrality
Water, the wheat straw waste after being handled.
(3) by relative to the NaOH of the over dry wheat straw quality 10% and Na relative to over dry wheat straw quality 10%2SO3It is dissolved in
In distilled water, then the mixed liquor is added in the wheat straw waste after treatment referred in step (2), adds distilled water,
It is 35% or so to make wheat straw waste mass concentration, is then handled again under constant temperature, and treatment temperature is 120 DEG C, processing time
2h, the wheat straw after being handled.
(4) wheat straw after Chemical impregnation processing in step (3) is carried out discongesting processing.Pressure grinding, mill are carried out first
Solution condition is 120 DEG C of temperature, and the MPa of pressure 0.3, the mass concentration of wheat straw slurry is 35%, grinding 10 minutes；Then normal pressure is carried out
Grinding, 120 DEG C of temperature, pressure is grinding, disk mill gap 0.3mm, the mass concentration 35% of wheat straw slurry, after must discongesting under normal pressure
Wheat straw slurry.
(5) wheat straw pulp fiber after grinding in step (5) is divided by screening and collecting by the sieve of 100 mesh
From it is 25% that the wheat straw slurry recycling being collected into, which is dehydrated to concentration, is then 60 by defibrination to beating degreeoSR.Receive
Wheat straw slurry after collection mashing, for follow-up enzymolysis.
(6) under sterile conditions, acetic acid one acetate buffers of the pH 5 or so is added into wheat straw slurry after mashing
Liquid, ratio is g:ML is 1:20, enzyme liquid is added after being sufficiently stirred for, then adding distilled water makes the mass concentration of substrate be 10%, nothing
Incubated enzymolysis is carried out under the conditions of bacterium, 50 DEG C of reaction temperature, reaction time 40h, pH=6 or so are cultivated；The rpm of rotating speed 120.Its
In, enzyme liquid is cellulase and cellulose dextranase complex enzyme, and the consumption of cellulase is 80CBU/g substrates, fiber used
The IU/mL of enzyme activity 45 of plain enzyme, temperature is 60 DEG C, and pH value is 6.0；Cellulose dextranase consumption is 33EGU/g substrates, fiber
The enzyme activity of plain dextranase is 800EGU/g, and temperature is 60 DEG C, and pH value is 6.0.
It can thus be concluded that, under above-mentioned enzyme hydrolysis condition:After hydrolysis 20 hours, the concentration of glucose is in hydrolyzate
7560mg/L, the inversion rate of glucose of substrate is 91%;After hydrolysis 30 hours, the concentration of glucose is 8020mg/L in hydrolyzate,
The inversion rate of glucose of substrate is 98.56%.
Comparative example
Using CN102517358A a kind of raising non-wood fibrous raw material Enzymatic hydrolysis efficiency preprocess method to stalk
Pre-processed, the yield of the glucose of substrate is between 59.9% ~ 83.2%, and the conversion ratio of its glucose is 54.3% ~ 75.9%
Between, and temperature be at 120 DEG C ~ 140 DEG C, compared to from the point of view of, inversion rate of glucose of the invention 73.62% ~ 98.56%,
And temperature control has saved energy consumption at 90 DEG C ~ 120 DEG C, improves the transformation efficiency of glucose.Using CN106011199A's
A kind of preprocess method of agricultural crop straw is pre-processed to stalk, last cellulase hydrolysis yield 79.3% ~ 84.78% it
Between, but the temperature conditionss are that for the present invention, energy consumption is of a relatively high between 140 DEG C ~ 160 DEG C.
Each embodiment contrast is shown in Table 1.
Table 1
| The concentration of glucose | The conversion ratio of the glucose of substrate（%） | Reaction condition | |
| Embodiment 1 | 5430 | 73.62 | Boiling temperature：90℃；Boiling concentration of substrate: 10% H2O2, 10% NaOH；Digestion time：1h；Digest pH:5；Beating degree：40 oSR；Digest cultivation temperature： 40℃；Digest pH 5；Enzymolysis time 30h. |
| Embodiment 2 | 6348 | 85.76 | Boiling temperature：100℃；Boiling concentration of substrate：15% H2O2, 20% NaOH；Digestion time：2h；Digest pH:5.5；Beating degree：50 oSR；Enzymolysis culture temperature Degree：45℃；Digest pH 5.5；Enzymolysis time 38h. |
| Embodiment 3 | 8020 | 98.56 | Boiling temperature：120℃；Boiling concentration of substrate:20% H2O2, 30% NaOH；Digestion time：3h；Digest pH:6；Beating degree：60oSR;Digest cultivation temperature： 50℃；Digest pH 6；Enzymolysis time 40h. |
| Comparative example CN102517358A | 54.3%~75.9% | 120℃~140℃ | |
| Comparative example CN106011199A | 79.3%~84.78% | 140℃~160℃ |
As can be seen here, the preprocess method that wheat straw waste of the invention is efficiently digested, can effectively improve glucose in hydrolyzate
Concentration and substrate glucose conversion ratio.The concentration of glucose can improve 10.0% ~ 20.0% in hydrolyzate；The grape of substrate
The conversion ratio of sugar can improve 13.78% ~ 29.85%.
Claims (10)
1. a kind of realize the preprocess method that wheat straw waste is efficiently digested, step is as follows：
(1) wheat straw waste is placed in 90-120 DEG C or so of water and soaked 30-60 minutes, then extrude the stalk soaked
Machine is extruded, and removes the moisture in stalk；H is sequentially added into the wheat straw waste after extruding2O2, NaOH, distilled water, so
After carry out boiling, temperature control is at 90-120 DEG C, and digestion time is 1-3h；
(2) step will be passed through（1）Pretreated wheat straw waste is placed in extruder to be extruded again, the wheat straw straw after extrusion process
Stalk, to neutrality, then carries out extrusion dehydration, the wheat straw waste after being handled again by water washing；
(3) by NaOH and Na2SO3Mixed liquor is added in the wheat straw waste after treatment described in step (2), then permanent again
Handled under the conditions of temperature, treatment temperature is 90 DEG C -120 DEG C, and processing time 1-2h obtains the wheat straw after alkali process；
(4) wheat straw after handling in step (3) is carried out discongesting processing：Pressure grinding, normal pressure grinding are sequentially passed through, must be discongested
Wheat straw slurry afterwards；
(5) wheat straw slurry after grinding in step (4) is separated by screening and collecting by the sieve of 80-100 mesh, will be received
The wheat straw slurry recycling integrated is dehydrated to concentration as 15%-25%, is then 40 by defibrination to beating degree oSR -60 oSR；
The wheat straw slurry after mashing is collected, for follow-up enzymolysis；
(6) digest：
Acetic acid one sodium acetate buffers of the pH in 4-5, the quality of the gentle fliud flushing of wheat straw slurry are added after to mashing in wheat straw slurry
Volume ratio is g:ML is 1:15-1:20, added after being sufficiently stirred for and incubated enzymolysis is carried out under enzyme liquid, aseptic condition, cultivated
40 DEG C -50 DEG C of reaction temperature, reaction time 36-40h, pH=5-6 or so；Rotating speed 100-120 rpm.
2. the preprocess method that now wheat straw waste is efficiently digested as claimed in claim 1, it is characterised in that step（1）Middle institute
That states adds H into the wheat straw waste after extruding2O2To be added into the wheat straw waste after extruding relative to over dry wheat straw
Stalk quality 10%-20% H2O2, the H2O2Mass percent concentration be 30%.
3. the preprocess method that now wheat straw waste is efficiently digested as claimed in claim 1 or 2, it is characterised in that step（1）In
It is described that to be directly added into NaOH into the wheat straw waste after extruding be to add relative to over dry wheat straw quality 10%-30%'s
NaOH。
4. the preprocess method that the existing wheat straw waste as described in any one of claim 1 ~ 3 is efficiently digested, it is characterised in that step
（1）Described in into the wheat straw waste after extruding add distilled water be, over dry wheat straw waste quality with add distilled water after
Liquid mass volume ratio be 1:5-1:10.
5. the preprocess method that the existing wheat straw waste as described in any one of claim 1 ~ 4 is efficiently digested, it is characterised in that step
(3) NaOH and Na described in2SO3Mixed liquor, by the NaOH relative to over dry wheat straw quality 5.0%-10% and relative to over dry wheat
Herbaceous stem amount 5.0%-10% Na2SO3It is dissolved in distilled water and obtains.
6. the preprocess method that the existing wheat straw waste as described in any one of claim 1 ~ 5 is efficiently digested, it is characterised in that step
(3) wheat straw waste mass concentration is 25%-35% in.
7. the preprocess method that the existing wheat straw waste as described in any one of claim 1 ~ 6 is efficiently digested, it is characterised in that step
(4) the pressure grinding described in, grinding condition is 90 DEG C -120 DEG C of temperature, and pressure 0.1-0.3 MPa, the quality of wheat straw slurry is dense
Spend for 25%-35%, grinding 5-10 minutes.
8. the preprocess method that the existing wheat straw waste as described in any one of claim 1 ~ 7 is efficiently digested, it is characterised in that step
(4) the normal pressure grinding described in, 90 DEG C -120 DEG C of temperature, pressure is grinding, disk mill gap 0.10-0.3mm, Straw Pulp under normal pressure
The mass concentration 25%-35% of material.
9. the preprocess method that the existing wheat straw waste as described in any one of claim 1 ~ 8 is efficiently digested, it is characterised in that step
(6) mass concentration of substrate is 5.0%-10% in.
10. the preprocess method that the existing wheat straw waste as described in any one of claim 1 ~ 9 is efficiently digested, it is characterised in that step
Suddenly the enzyme liquid described in (6) is cellulase and cellulose dextranase complex enzyme, and the consumption of cellulase is 76 ~ 84CBU/g
Substrate, the enzyme activity 35-45 IU/mL of cellulase used, temperature is 50 DEG C -60 DEG C, and pH value is 4.5-6.0；Cellulose glucan
Enzyme dosage is 31-33EGU/g substrates, and the enzyme activity of cellulose dextranase is 600-800EGU/g, and temperature is 50-60 DEG C, pH value
For 4.5-6.0.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107828832A (en) * | 2017-11-21 | 2018-03-23 | 中国农业科学院麻类研究所 | A kind of method for improving crudefiber crop stalk enzymatic saccharification efficiency |
| CN109336668A (en) * | 2018-08-23 | 2019-02-15 | 刘丰恺 | The extraction process of rice seedling raising ground substance plate cellulose |
| CN109503859A (en) * | 2018-12-06 | 2019-03-22 | 齐鲁工业大学 | A method of highly sulfonated sodium lignosulfonate is prepared using wheat stalk |
| CN114875105A (en) * | 2022-03-09 | 2022-08-09 | 益水源生物技术(西安)有限公司 | Biomass-based efficient carbon source and preparation method thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103497982A (en) * | 2013-09-03 | 2014-01-08 | 天津科技大学 | Pretreatment method assists in realizing wheat straw high-efficiency enzymatic hydrolysis conversion |
| CN105039457A (en) * | 2015-07-13 | 2015-11-11 | 河南德邻生物制品有限公司 | Novel process for jointly degrading and saccharifying crop straw by means of thermo-chemical treatment, microbial fermentation and enzymatic hydrolysis |
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| CN103497982A (en) * | 2013-09-03 | 2014-01-08 | 天津科技大学 | Pretreatment method assists in realizing wheat straw high-efficiency enzymatic hydrolysis conversion |
| CN105039457A (en) * | 2015-07-13 | 2015-11-11 | 河南德邻生物制品有限公司 | Novel process for jointly degrading and saccharifying crop straw by means of thermo-chemical treatment, microbial fermentation and enzymatic hydrolysis |
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| 姜锡瑞: "《生物发酵产业技术》", 31 May 2016, 中国轻工业出版社 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107828832A (en) * | 2017-11-21 | 2018-03-23 | 中国农业科学院麻类研究所 | A kind of method for improving crudefiber crop stalk enzymatic saccharification efficiency |
| CN109336668A (en) * | 2018-08-23 | 2019-02-15 | 刘丰恺 | The extraction process of rice seedling raising ground substance plate cellulose |
| CN109503859A (en) * | 2018-12-06 | 2019-03-22 | 齐鲁工业大学 | A method of highly sulfonated sodium lignosulfonate is prepared using wheat stalk |
| CN109503859B (en) * | 2018-12-06 | 2021-04-27 | 齐鲁工业大学 | Method for preparing sodium lignosulfonate with high sulfonation degree by using wheat straws |
| CN114875105A (en) * | 2022-03-09 | 2022-08-09 | 益水源生物技术(西安)有限公司 | Biomass-based efficient carbon source and preparation method thereof |
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