TH10632C3 - Reagent set (Gnathostomiasis) - Google Patents

Abstract

DC60 (20/08/57) reagent set (gnathostomiasis) consists of antigen strips, A is a reagent used to absorb antibodies against bacteria, B is a reagent used to wash antigen sheets, C reagent solution, enzyme conjugate, D reagent. Is a solution used to dissolve substrate, E is a hydrogen peroxide solution (hydrogen peroxide; H2O2) 30% concentration substrate, F reagent positive control agent, G reagent negative control agent, reaction tray, test tube, color chart and tweezer ( forceps), the gnathostomiasis reagent kit is a kit for detecting specific antibodies in the serum of patients infected with Gnathostoma spinigerum, a type of cutaneous gnathostomiasis in Brain and / or spinal cord (cerebral and cerebrospinal gnathostomiasis) and in the internal organs (visceral gnathostomiasis) by giving the validity Sensitivity, specificity Positive and negative prediction values were 97.4, 100, 96.1, 92.6, and 100, respectively. The results were read visually and reported at 2 hours. (Gnathoustomisais) consists of antigen strips, A is a reagent used to absorb antibodies against bacteria, B is a reagent used to wash the antigen plate, C reagent is an enzyme conjugate solution, D is a reagent. For dissolving substrates (substrate) E solution is a solution of 30% concentrated hydrogen peroxide (hydrogen peroxide; H2O2) solution F is a positive control agent, G is a negative control agent. The gnathostomiasis reagent kit is a reagent for the detection of specific antibodies in the infected patient's serum. Gnathostoma spinigerum (cutaneous gnathostomiasis) in the brain and / or spinal cord (cerebral and cerebrospinal gnathostomiasis) and in the visceral gnathostomiasis. Sensitivity, specificity Positive and negative prediction values 97.4,100,96.1,92.6 and 100 percent respectively, read the results visually and report the results in 2 hours.

Claims (1)

1. Reagent test kit (Gnathoustomisais) consisted of - A number of antigen strips, a membrane containing proteins from the matrix metalloproteinase gene of the Gnatostoma spinigerum, are attached. Reagents used to absorb antibodies against bacteria. Which consists of Escherichia coli lyaste 1% in a solution of PBS PH 7.5 (0.1M PBS, pH 7.5) 0.1 molar concentration - B solution is Solution used to clean the antigen sheet It contains 0.1% Tween 20 (Tween 20) and 1% low fat skimmed milk powder in a 0.1 molar concentration of PBS pH 7.5 (PBS, pH 7.5) solution. C is an enzyme conjugate solution, a secondary antibody solution labeled with horseradish peroxidase (HRP), which include 2nd order antibodies labeled with enzyme There were 0.005% and low fat skimmed milk powder (1%) in 0.1 molar concentration of PBS pH 7.5 solution (0.1M (PBS, pH 7.5). Dissolve substrate, which is 50 mM Tris pH 7.6 (50 mM Tris HCl, pH 7.6) - E solution is an icrogen peroxide solution (hydrogen peroxide; H2O2). Concentration 30% - substrate (substrate) is diaminobenzidine. Tetrahydrochloride (diaminobenzidine tetrahydrochloride; DAB) - F reagent is a positive control agent, a total serum positive for the detection of band-specific antibodies. 32 kT antigen-G reagent is a controlled substance. Negative is the total serum negative for the detection of band-specific antibodies. 32 kilotons of antigens - A number of reaction trays are trays with a number of grooves. That are marked to represent a test of positive results (positive test), negative test (negative test) and test samples to be tested (sample test) - a number of test tubes. That are marked to indicate a test positive results (positive test), a positive test (negative test) and test the sample to be tested (sample test) - color chart (color chart) - tweezer (forceps. A number of